AlfalfaGEDB | MsG0580024744.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024744.01

KEGG: K18811
GO: -
PFAM: PF00134

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024744.01.T01	XP_003611595.1	73.93	257	24	3	14	227	97	353	2.91E-126	371

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024744.01.T01	Q2V3B2	31.797	217	113	9	34	226	117	322	2.08E-22	96.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024744.01.T01	G7K3T3	73.930	257	24	3	14	227	97	353	1.39e-126	371

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480023620.01	MsG0580024744.01	0.801809	7.557635e-49	3.643293e-46
MsG0580024489.01	MsG0580024744.01	0.811035	8.561307e-51	5.243732e-48
MsG0580024743.01	MsG0580024744.01	0.816574	5.154219e-52	3.665872e-49
MsG0580024744.01	MsG0780039655.01	0.817631	2.982791e-52	2.183707e-49
MsG0580024744.01	MsG0780039656.01	0.824160	9.384041e-54	8.246398e-51
MsG0580024744.01	MsG0780039712.01	0.802996	4.303039e-49	2.137898e-46
MsG0580024744.01	MsG0880043516.01	0.804014	2.647018e-49	1.350055e-46
MsG0580024744.01	MsG0880047226.01	0.803144	4.010580e-49	2.000197e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024744.01.T01	MTR_5g015670	73.930	257	24	3	14	227	97	353	3.53e-130	371
MsG0580024744.01.T01	MTR_3g103080	35.021	237	107	5	16	207	63	297	4.27e-31	116
MsG0580024744.01.T01	MTR_4g095790	36.321	212	108	9	29	219	108	313	3.96e-28	108
MsG0580024744.01.T01	MTR_3g100710	34.682	173	97	4	18	174	95	267	7.15e-21	89.4
MsG0580024744.01.T01	MTR_3g102310	38.462	130	67	3	28	146	122	249	4.23e-20	87.4
MsG0580024744.01.T01	MTR_4g080990	35.443	158	84	6	28	168	129	285	6.95e-18	81.3
MsG0580024744.01.T01	MTR_8g063120	40.385	156	76	6	30	168	133	288	3.73e-17	79.3
MsG0580024744.01.T01	MTR_8g063120	46.739	92	45	2	30	117	133	224	3.87e-17	78.6
MsG0580024744.01.T01	MTR_3g100710	33.742	163	92	4	28	174	134	296	5.51e-17	79.0
MsG0580024744.01.T01	MTR_4g094942	34.810	158	83	7	28	166	119	275	7.77e-17	78.6
MsG0580024744.01.T01	MTR_1g107535	35.338	133	61	3	28	144	144	267	1.12e-16	78.2
MsG0580024744.01.T01	MTR_5g035360	43.878	98	43	2	30	115	116	213	1.32e-16	77.8
MsG0580024744.01.T01	MTR_2g011500	31.844	179	96	7	28	187	121	292	2.98e-15	73.6
MsG0580024744.01.T01	MTR_5g032550	44.872	78	40	1	28	102	129	206	1.62e-14	71.2
MsG0580024744.01.T01	MTR_5g032550	44.872	78	40	1	28	102	129	206	3.09e-14	71.2
MsG0580024744.01.T01	MTR_5g065830	42.169	83	44	2	32	111	129	210	2.89e-13	68.2
MsG0580024744.01.T01	MTR_2g089490	31.056	161	84	5	30	168	88	243	5.54e-13	67.0
MsG0580024744.01.T01	MTR_2g089490	31.098	164	83	6	30	168	88	246	1.96e-12	65.5
MsG0580024744.01.T01	MTR_8g068950	32.692	156	77	7	28	167	145	288	2.75e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024744.01.T01	AT4G37630	31.797	217	113	9	34	226	117	322	2.12e-23	96.3
MsG0580024744.01.T01	AT4G37630	55.422	83	35	1	29	109	110	192	1.54e-22	92.8
MsG0580024744.01.T01	AT4G37630	55.422	83	35	1	29	109	112	194	1.87e-22	92.8
MsG0580024744.01.T01	AT4G34160	33.333	165	75	5	28	166	124	279	9.21e-20	87.0
MsG0580024744.01.T01	AT5G65420	39.669	121	61	4	31	141	118	236	5.34e-18	80.9
MsG0580024744.01.T01	AT5G65420	39.669	121	61	4	31	141	128	246	8.44e-18	80.9
MsG0580024744.01.T01	AT5G65420	39.669	121	61	4	31	141	118	236	8.76e-18	80.9
MsG0580024744.01.T01	AT2G22490	43.956	91	47	2	30	117	136	225	1.43e-17	80.9
MsG0580024744.01.T01	AT2G22490	43.956	91	47	2	30	117	136	225	1.49e-17	80.5
MsG0580024744.01.T01	AT1G70210	39.837	123	57	3	30	140	121	238	2.31e-17	79.7
MsG0580024744.01.T01	AT5G10440	40.164	122	59	5	31	141	109	227	9.72e-17	77.8
MsG0580024744.01.T01	AT5G67260	30.851	188	99	5	31	191	136	319	1.04e-16	78.6
MsG0580024744.01.T01	AT5G67260	30.851	188	99	5	31	191	136	319	1.46e-16	77.8
MsG0580024744.01.T01	AT3G50070	30.818	159	87	5	28	166	123	278	3.40e-16	76.6
MsG0580024744.01.T01	AT5G10440	44.578	83	42	2	31	110	109	190	7.11e-16	74.3
MsG0580024744.01.T01	AT5G65420	40.659	91	49	2	31	116	118	208	1.56e-15	73.6
MsG0580024744.01.T01	AT4G03270	28.931	159	93	6	30	169	96	253	5.25e-14	70.1
MsG0580024744.01.T01	AT4G03270	29.114	158	92	6	31	169	96	252	8.58e-14	69.3
MsG0580024744.01.T01	AT5G02110	27.778	144	97	4	16	153	74	216	3.40e-11	62.0

Find 29 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGTACTTTGGAATGGAAAA+TGG	0.144972	5:+9107854	MsG0580024744.01.T01:CDS
TGATTCAAGCAAAAGCTTTC+TGG	0.195049	5:+9107347	MsG0580024744.01.T01:CDS
TCTTGATCCATTGCAGAATT+TGG	0.222300	5:-9107916	None:intergenic
TTCTCAAATCGATATTCTAT+TGG	0.242040	5:-9107798	None:intergenic
TCCGAAGATCAATTTCCTTT+TGG	0.310681	5:-9108156	None:intergenic
AGTTAACAAGACACCTTTAT+CGG	0.362687	5:+9108359	MsG0580024744.01.T01:CDS
GTTGATAATAAATCCGATAA+AGG	0.374551	5:-9108372	None:intergenic
AGTACTTTGGAATGGAAAAT+GGG	0.395838	5:+9107855	MsG0580024744.01.T01:CDS
CTCGTTTGCATGCTATTGAT+TGG	0.395951	5:+9107380	MsG0580024744.01.T01:CDS
TCTATTGGATATTCTGATAG+TGG	0.404279	5:-9107783	None:intergenic
TAAGAGAAAGCTTGGTTATG+AGG	0.430380	5:+9108452	MsG0580024744.01.T01:CDS
ACCAAAAGGAAATTGATCTT+CGG	0.444150	5:+9108155	MsG0580024744.01.T01:CDS
AAATAATGCAAATAAGCAAA+AGG	0.447047	5:-9107888	None:intergenic
AATAATGCAAATAAGCAAAA+GGG	0.449345	5:-9107887	None:intergenic
TATGCAGGAATCAAAACCTT+GGG	0.450693	5:+9107695	MsG0580024744.01.T01:intron
TTGATTCCACATTAACCAAA+AGG	0.453404	5:+9108141	MsG0580024744.01.T01:CDS
GAAGCAGAAGCAATTATAGA+TGG	0.470085	5:-9108104	None:intergenic
GGCTATTCAATTGTTGTCAG+TGG	0.484737	5:+9107716	MsG0580024744.01.T01:CDS
AACAAATCTAATGCTTGTGC+AGG	0.488051	5:+9108426	MsG0580024744.01.T01:CDS
GCAGGAGTTAAGAGAAAGCT+TGG	0.497942	5:+9108444	MsG0580024744.01.T01:CDS
TTCTTCACCAAATTCTGCAA+TGG	0.509880	5:+9107909	MsG0580024744.01.T01:CDS
TTATGCAGGAATCAAAACCT+TGG	0.516551	5:+9107694	MsG0580024744.01.T01:intron
TGCAAATAAGCAAAAGGGGT+TGG	0.517338	5:-9107882	None:intergenic
GCCACAACACATTGTGACAT+TGG	0.560442	5:+9107960	MsG0580024744.01.T01:CDS
ACCAATGTCACAATGTGTTG+TGG	0.573841	5:-9107961	None:intergenic
GAATAAAGTTATTAAGAACA+TGG	0.585219	5:+9107818	MsG0580024744.01.T01:CDS
ATAATGCAAATAAGCAAAAG+GGG	0.614789	5:-9107886	None:intergenic
GACAACAATTGAATAGCCCA+AGG	0.617594	5:-9107711	None:intergenic
ATTGGATATTCTGATAGTGG+AGG	0.627410	5:-9107780	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTTATTTTATTTTATGC+AGG	+	Chr5:9107680-9107699	MsG0580024744.01.T01:intron	10.0%
!!!	TTATCATTAATTATTTTGAT+CGG	+	Chr5:9107562-9107581	MsG0580024744.01.T01:intron	10.0%
!!!	AAAACAAATTTTGATTAGTT+TGG	-	Chr5:9107428-9107447	None:intergenic	15.0%
!!	AAATAATGCAAATAAGCAAA+AGG	-	Chr5:9107891-9107910	None:intergenic	20.0%
!!	AATAATGCAAATAAGCAAAA+GGG	-	Chr5:9107890-9107909	None:intergenic	20.0%
!!	GAATAAAGTTATTAAGAACA+TGG	+	Chr5:9107818-9107837	MsG0580024744.01.T01:CDS	20.0%
!	AAAACAATGAAAGAGGAAAA+AGG	-	Chr5:9107462-9107481	None:intergenic	25.0%
!	ATAATGCAAATAAGCAAAAG+GGG	-	Chr5:9107889-9107908	None:intergenic	25.0%
!	TTCTCAAATCGATATTCTAT+TGG	-	Chr5:9107801-9107820	None:intergenic	25.0%
!	TTTATCAATTGCAGCAAAAA+TGG	+	Chr5:9107743-9107762	MsG0580024744.01.T01:CDS	25.0%
!!	ATTAGTTTAATCTCATCATG+TGG	+	Chr5:9108178-9108197	MsG0580024744.01.T01:intron	25.0%
!!!	ACTTTTGATTTTGAGTACTT+TGG	+	Chr5:9107842-9107861	MsG0580024744.01.T01:CDS	25.0%
!!!	GTTGATAATAAATCCGATAA+AGG	-	Chr5:9108375-9108394	None:intergenic	25.0%
!!!	TTGTTTTTGTTTTATGCAAC+AGG	+	Chr5:9108287-9108306	MsG0580024744.01.T01:intron	25.0%
!!!	TTTCCTCTTTCATTGTTTTT+TGG	+	Chr5:9107463-9107482	MsG0580024744.01.T01:intron	25.0%
	ACCAAAAGGAAATTGATCTT+CGG	+	Chr5:9108155-9108174	MsG0580024744.01.T01:CDS	30.0%
	AGTTAACAAGACACCTTTAT+CGG	+	Chr5:9108359-9108378	MsG0580024744.01.T01:CDS	30.0%
	CAACCAAAAAACAATGAAAG+AGG	-	Chr5:9107469-9107488	None:intergenic	30.0%
	TCTATTGGATATTCTGATAG+TGG	-	Chr5:9107786-9107805	None:intergenic	30.0%
	TGTTAAGCTCTTTACATCAA+TGG	-	Chr5:9107604-9107623	None:intergenic	30.0%
	TTGATTCCACATTAACCAAA+AGG	+	Chr5:9108141-9108160	MsG0580024744.01.T01:CDS	30.0%
!	TTGATCGGTTTCTTTCAAAA+CGG	+	Chr5:9107577-9107596	MsG0580024744.01.T01:intron	30.0%
!!	AGTACTTTGGAATGGAAAAT+GGG	+	Chr5:9107855-9107874	MsG0580024744.01.T01:CDS	30.0%
!!	CAATTTCCTTTTGGTTAATG+TGG	-	Chr5:9108150-9108169	None:intergenic	30.0%
!!	TGATTTTGAGTACTTTGGAA+TGG	+	Chr5:9107847-9107866	MsG0580024744.01.T01:CDS	30.0%
!!	TTATTTGTTGATTCAGACAC+AGG	+	Chr5:9107508-9107527	MsG0580024744.01.T01:intron	30.0%
	AACAAATCTAATGCTTGTGC+AGG	+	Chr5:9108426-9108445	MsG0580024744.01.T01:CDS	35.0%
	GAAGCAGAAGCAATTATAGA+TGG	-	Chr5:9108107-9108126	None:intergenic	35.0%
	TAAGAGAAAGCTTGGTTATG+AGG	+	Chr5:9108452-9108471	MsG0580024744.01.T01:CDS	35.0%
	TATGCAGGAATCAAAACCTT+GGG	+	Chr5:9107695-9107714	MsG0580024744.01.T01:intron	35.0%
	TCTTGATCCATTGCAGAATT+TGG	-	Chr5:9107919-9107938	None:intergenic	35.0%
	TGATTCAAGCAAAAGCTTTC+TGG	+	Chr5:9107347-9107366	MsG0580024744.01.T01:CDS	35.0%
	TTATGCAGGAATCAAAACCT+TGG	+	Chr5:9107694-9107713	MsG0580024744.01.T01:intron	35.0%
	TTCTTCACCAAATTCTGCAA+TGG	+	Chr5:9107909-9107928	MsG0580024744.01.T01:CDS	35.0%
!	AACATTGAAGATTTTCCTCC+AGG	+	Chr5:9108480-9108499	MsG0580024744.01.T01:CDS	35.0%
!	TCCGAAGATCAATTTCCTTT+TGG	-	Chr5:9108159-9108178	None:intergenic	35.0%
!!	ATTGGATATTCTGATAGTGG+AGG	-	Chr5:9107783-9107802	None:intergenic	35.0%
!!	GAGTACTTTGGAATGGAAAA+TGG	+	Chr5:9107854-9107873	MsG0580024744.01.T01:CDS	35.0%
	ACCAATGTCACAATGTGTTG+TGG	-	Chr5:9107964-9107983	None:intergenic	40.0%
	CACATTGTGACATTGGTCAA+AGG	+	Chr5:9107967-9107986	MsG0580024744.01.T01:intron	40.0%
	CTCGTTTGCATGCTATTGAT+TGG	+	Chr5:9107380-9107399	MsG0580024744.01.T01:CDS	40.0%
	GACAACAATTGAATAGCCCA+AGG	-	Chr5:9107714-9107733	None:intergenic	40.0%
	GATTCAGACACAGGCAAAAT+TGG	+	Chr5:9107517-9107536	MsG0580024744.01.T01:intron	40.0%
	GGCTATTCAATTGTTGTCAG+TGG	+	Chr5:9107716-9107735	MsG0580024744.01.T01:CDS	40.0%
	TGCAAATAAGCAAAAGGGGT+TGG	-	Chr5:9107885-9107904	None:intergenic	40.0%
!	GTTTTCAACAACAACACTCG+TGG	-	Chr5:9108409-9108428	None:intergenic	40.0%
	GCAGGAGTTAAGAGAAAGCT+TGG	+	Chr5:9108444-9108463	MsG0580024744.01.T01:CDS	45.0%
	GCCACAACACATTGTGACAT+TGG	+	Chr5:9107960-9107979	MsG0580024744.01.T01:CDS	45.0%
!	GGTTGACGTAGCTTTTGTCC+TGG	-	Chr5:9108501-9108520	None:intergenic	50.0%
!	TGACGTAGCTTTTGTCCTGG+AGG	-	Chr5:9108498-9108517	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	9107325	9108524	9107325	ID=MsG0580024744.01;Name=MsG0580024744.01
Chr5	mRNA	9107325	9108524	9107325	ID=MsG0580024744.01.T01;Parent=MsG0580024744.01;Name=MsG0580024744.01.T01;_AED=0.25;_eAED=0.25;_QI=0\|0\|0\|1\|1\|1\|4\|0\|227
Chr5	exon	9107325	9107411	9107325	ID=MsG0580024744.01.T01:exon:1821;Parent=MsG0580024744.01.T01
Chr5	exon	9107702	9107981	9107702	ID=MsG0580024744.01.T01:exon:1822;Parent=MsG0580024744.01.T01
Chr5	exon	9108081	9108181	9108081	ID=MsG0580024744.01.T01:exon:1823;Parent=MsG0580024744.01.T01
Chr5	exon	9108309	9108524	9108309	ID=MsG0580024744.01.T01:exon:1824;Parent=MsG0580024744.01.T01
Chr5	CDS	9107325	9107411	9107325	ID=MsG0580024744.01.T01:cds;Parent=MsG0580024744.01.T01
Chr5	CDS	9107702	9107981	9107702	ID=MsG0580024744.01.T01:cds;Parent=MsG0580024744.01.T01
Chr5	CDS	9108081	9108181	9108081	ID=MsG0580024744.01.T01:cds;Parent=MsG0580024744.01.T01
Chr5	CDS	9108309	9108524	9108309	ID=MsG0580024744.01.T01:cds;Parent=MsG0580024744.01.T01

Gene Sequence

>MsG0580024744.01.T01

ATGATGTTGATGTTGAAGATGATGATTCAAGCAAAAGCTTTCTGGTTGAGAAATGCTCGTTTGCATGCTATTGATTGGATTTTCAATGAATCAAAACCTTGGGCTATTCAATTGTTGTCAGTGGCATGTTTATCAATTGCAGCAAAAATGGAAGAACAAAGTGTTCCTCCACTATCAGAATATCCAATAGAATATCGATTTGAGAATAAAGTTATTAAGAACATGGAACTTTTGATTTTGAGTACTTTGGAATGGAAAATGGGATTACCAACCCCTTTTGCTTATTTGCATTATTTCTTCACCAAATTCTGCAATGGATCAAGATCAGAGATAATAATCACAAAAGCCACAACACATTGTGACATTGATTTTAATTTGATTAATCAGAGACCATCTATAATTGCTTCTGCTTCTATATTAGCAGCTTTTGATTCCACATTAACCAAAAGGAAATTGATCTTCGGATTAGAGCATATATTTTCATGTTACAATGTTATACAAGAAAAAATGAGAAACAAAGTTAACAAGACACCTTTATCGGATTTATTATCAACAGAATCAAATTCCACGAGTGTTGTTGTTGAAAACAAATCTAATGCTTGTGCAGGAGTTAAGAGAAAGCTTGGTTATGAGGACATAAACATTGAAGATTTTCCTCCAGGACAAAAGCTACGTCAACCTTAA

Protein sequence

>MsG0580024744.01.T01

MMLMLKMMIQAKAFWLRNARLHAIDWIFNESKPWAIQLLSVACLSIAAKMEEQSVPPLSEYPIEYRFENKVIKNMELLILSTLEWKMGLPTPFAYLHYFFTKFCNGSRSEIIITKATTHCDIDFNLINQRPSIIASASILAAFDSTLTKRKLIFGLEHIFSCYNVIQEKMRNKVNKTPLSDLLSTESNSTSVVVENKSNACAGVKRKLGYEDINIEDFPPGQKLRQP*