AlfalfaGEDB | MsG0580024848.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024848.01

KEGG: -
GO: -
PFAM: PF03195

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024848.01.T01	XP_003611797.1	95.935	123	5	0	7	129	25	147	2.30E-78	244

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024848.01.T01	Q9FKZ3	71.875	96	27	0	7	102	24	119	1.29E-43	147

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024848.01.T01	G7K830	95.935	123	5	0	7	129	25	147	1.10e-78	244

TFs/TRs:

Gene ID	Type	Classification
MsG0580024848.01.T01	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0580024848.01	MsG0580025633.01	0.841281	5.225557e-58	7.640130e-55
MsG0580024848.01	MsG0680030296.01	0.803242	3.826808e-49	1.913383e-46
MsG0580024848.01	MsG0880043495.01	0.801944	7.090611e-49	3.429742e-46
MsG0380016250.01	MsG0580024848.01	0.800571	1.353790e-48	6.324960e-46
MsG0380017594.01	MsG0580024848.01	0.811757	5.967192e-51	3.724965e-48
MsG0380017826.01	MsG0580024848.01	0.819766	9.776297e-53	7.592684e-50
MsG0280007199.01	MsG0580024848.01	0.820439	6.855813e-53	5.424868e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024848.01.T01	MTR_5g017950	95.935	123	5	0	7	129	25	147	2.78e-82	244
MsG0580024848.01.T01	MTR_3g073690	73.737	99	26	0	7	105	26	124	8.75e-48	152
MsG0580024848.01.T01	MTR_3g452660	68.421	95	29	1	7	100	24	118	7.55e-38	130
MsG0580024848.01.T01	MTR_4g105170	62.069	87	33	0	7	93	28	114	2.23e-32	113
MsG0580024848.01.T01	MTR_3g077240	59.770	87	35	0	7	93	27	113	4.27e-31	110
MsG0580024848.01.T01	MTR_7g033800	58.621	87	36	0	7	93	28	114	1.06e-30	108
MsG0580024848.01.T01	MTR_5g083230	53.774	106	48	1	7	112	23	127	1.48e-29	105
MsG0580024848.01.T01	MTR_5g083960	57.471	87	37	0	7	93	56	142	1.53e-28	103
MsG0580024848.01.T01	MTR_6g005070	57.143	84	36	0	7	90	26	109	2.06e-28	101
MsG0580024848.01.T01	MTR_5g083010	57.647	85	36	0	7	91	28	112	1.15e-27	100
MsG0580024848.01.T01	MTR_2g068760	55.952	84	37	0	7	90	30	113	1.72e-27	99.8
MsG0580024848.01.T01	MTR_8g040900	65.517	87	30	0	7	93	24	110	2.01e-27	101
MsG0580024848.01.T01	MTR_8g079660	50.000	90	45	0	11	100	29	118	5.55e-27	101
MsG0580024848.01.T01	MTR_3g071590	55.952	84	37	0	7	90	25	108	5.99e-27	98.2
MsG0580024848.01.T01	MTR_3g094690	55.172	87	39	0	7	93	64	150	7.29e-27	100
MsG0580024848.01.T01	MTR_6g005080	52.874	87	41	0	7	93	27	113	7.79e-27	97.4
MsG0580024848.01.T01	MTR_3g031660	53.571	84	39	0	7	90	24	107	7.90e-27	98.6
MsG0580024848.01.T01	MTR_3g071420	57.317	82	35	0	7	88	31	112	1.02e-26	98.6
MsG0580024848.01.T01	MTR_4g083680	56.627	83	36	0	7	89	32	114	2.65e-26	97.1
MsG0580024848.01.T01	MTR_8g079620	45.545	101	55	0	2	102	20	120	3.86e-26	98.2
MsG0580024848.01.T01	MTR_8g079580	43.925	107	54	1	2	102	15	121	1.79e-24	94.0
MsG0580024848.01.T01	MTR_3g094690	54.023	87	39	1	7	93	64	149	2.42e-24	93.6
MsG0580024848.01.T01	MTR_2g093310	50.000	80	40	0	11	90	54	133	2.80e-23	90.1
MsG0580024848.01.T01	MTR_5g075020	46.847	111	59	0	7	117	60	170	5.89e-23	90.1
MsG0580024848.01.T01	MTR_7g096530	44.144	111	59	1	7	117	35	142	6.51e-23	89.7
MsG0580024848.01.T01	MTR_5g075020	46.847	111	59	0	7	117	64	174	6.85e-23	89.7
MsG0580024848.01.T01	MTR_1g095850	51.250	80	39	0	11	90	93	172	8.37e-23	89.4
MsG0580024848.01.T01	MTR_4g060950	48.750	80	41	0	11	90	46	125	2.32e-22	87.4
MsG0580024848.01.T01	MTR_8g036085	54.667	75	34	0	19	93	53	127	2.56e-21	85.9
MsG0580024848.01.T01	MTR_5g093010	40.659	91	54	0	3	93	18	108	2.97e-20	80.9
MsG0580024848.01.T01	MTR_7g028905	42.353	85	49	0	7	91	22	106	4.05e-20	79.7
MsG0580024848.01.T01	MTR_8g036105	46.535	101	47	2	7	106	35	129	4.72e-20	82.0
MsG0580024848.01.T01	MTR_6g027710	47.059	85	44	1	7	90	57	141	6.16e-20	82.0
MsG0580024848.01.T01	MTR_7g074990	54.167	72	33	0	19	90	50	121	8.59e-20	81.6
MsG0580024848.01.T01	MTR_1g070220	41.803	122	59	3	7	122	36	151	1.29e-19	80.9
MsG0580024848.01.T01	MTR_6g027700	35.385	130	82	2	3	130	20	149	4.43e-18	76.6
MsG0580024848.01.T01	MTR_5g080470	44.578	83	45	1	3	84	20	102	6.77e-18	75.9
MsG0580024848.01.T01	MTR_7g096610	43.137	102	50	2	19	112	38	139	1.51e-17	75.5
MsG0580024848.01.T01	MTR_7g075230	45.556	90	43	1	8	91	27	116	6.75e-17	73.6
MsG0580024848.01.T01	MTR_1g070205	47.945	73	38	0	19	91	42	114	7.44e-17	73.9
MsG0580024848.01.T01	MTR_6g011250	41.463	82	47	1	11	91	30	111	1.00e-13	64.7
MsG0580024848.01.T01	MTR_6g011200	34.211	114	67	4	3	108	21	134	1.08e-13	65.9
MsG0580024848.01.T01	MTR_4g099230	32.143	112	71	3	11	121	23	130	1.46e-13	63.9
MsG0580024848.01.T01	MTR_6g011230	37.662	77	48	0	11	87	32	108	1.60e-13	64.7
MsG0580024848.01.T01	MTR_4g107450	36.250	80	51	0	11	90	31	110	1.19e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024848.01.T01	AT5G66870	71.875	96	27	0	7	102	24	119	1.32e-44	147
MsG0580024848.01.T01	AT2G23660	60.215	93	37	0	7	99	22	114	1.00e-34	122
MsG0580024848.01.T01	AT2G23660	60.215	93	37	0	7	99	22	114	1.00e-34	122
MsG0580024848.01.T01	AT2G23660	60.215	93	37	0	7	99	22	114	1.00e-34	122
MsG0580024848.01.T01	AT5G63090	55.446	101	45	0	7	107	28	128	4.53e-32	112
MsG0580024848.01.T01	AT5G63090	55.446	101	45	0	7	107	28	128	4.53e-32	112
MsG0580024848.01.T01	AT5G63090	55.446	101	45	0	7	107	28	128	4.53e-32	112
MsG0580024848.01.T01	AT5G63090	55.446	101	45	0	7	107	28	128	4.53e-32	112
MsG0580024848.01.T01	AT1G65620	68.966	87	27	0	7	93	26	112	8.32e-30	107
MsG0580024848.01.T01	AT1G65620	68.966	87	27	0	7	93	26	112	8.32e-30	107
MsG0580024848.01.T01	AT1G65620	68.966	87	27	0	7	93	26	112	8.32e-30	107
MsG0580024848.01.T01	AT1G65620	68.966	87	27	0	7	93	26	112	8.32e-30	107
MsG0580024848.01.T01	AT1G65620	68.966	87	27	0	7	93	26	112	8.32e-30	107
MsG0580024848.01.T01	AT3G27650	53.763	93	43	0	3	95	52	144	3.33e-29	104
MsG0580024848.01.T01	AT2G30130	57.143	84	36	0	7	90	25	108	2.99e-27	100
MsG0580024848.01.T01	AT2G30340	54.762	84	38	0	7	90	69	152	3.90e-27	101
MsG0580024848.01.T01	AT2G30340	54.762	84	38	0	7	90	70	153	4.71e-27	101
MsG0580024848.01.T01	AT1G16530	53.571	84	39	0	7	90	32	115	4.10e-25	94.0
MsG0580024848.01.T01	AT1G31320	62.857	70	26	0	7	76	30	99	1.13e-24	92.8
MsG0580024848.01.T01	AT3G11090	51.685	89	43	0	7	95	28	116	3.55e-24	91.7
MsG0580024848.01.T01	AT1G07900	42.105	114	64	1	4	115	47	160	3.74e-24	92.0
MsG0580024848.01.T01	AT3G26620	45.349	86	47	0	7	92	22	107	2.31e-23	88.2
MsG0580024848.01.T01	AT2G40470	48.113	106	52	1	7	112	37	139	2.39e-23	90.1
MsG0580024848.01.T01	AT3G26660	45.349	86	47	0	7	92	22	107	2.43e-23	88.2
MsG0580024848.01.T01	AT4G00210	52.174	92	38	1	12	103	34	119	4.12e-23	90.1
MsG0580024848.01.T01	AT2G40470	48.113	106	52	1	7	112	62	164	4.38e-23	90.1
MsG0580024848.01.T01	AT2G28500	50.000	80	40	0	11	90	76	155	5.19e-22	87.4
MsG0580024848.01.T01	AT3G03760	50.588	85	41	1	7	90	68	152	2.72e-21	86.7
MsG0580024848.01.T01	AT5G35900	40.000	120	65	3	7	124	22	136	5.75e-21	84.3
MsG0580024848.01.T01	AT4G00220	55.405	74	33	0	19	92	47	120	6.34e-21	84.7
MsG0580024848.01.T01	AT2G45410	45.794	107	46	2	18	118	45	145	1.60e-20	82.8
MsG0580024848.01.T01	AT3G50510	37.903	124	72	3	7	128	29	149	5.59e-19	79.0
MsG0580024848.01.T01	AT3G50510	37.903	124	72	3	7	128	29	149	5.59e-19	79.0
MsG0580024848.01.T01	AT2G45420	51.351	74	36	0	19	92	67	140	5.65e-19	80.1
MsG0580024848.01.T01	AT2G42430	46.667	90	47	1	3	91	28	117	1.94e-18	78.6
MsG0580024848.01.T01	AT3G58190	48.649	74	38	0	18	91	40	113	1.24e-17	75.9
MsG0580024848.01.T01	AT2G31310	37.736	106	59	2	7	112	28	126	6.50e-17	73.2
MsG0580024848.01.T01	AT5G06080	51.515	66	32	0	18	83	36	101	4.78e-15	68.2
MsG0580024848.01.T01	AT2G42440	45.070	71	39	0	19	89	37	107	1.64e-14	67.8
MsG0580024848.01.T01	AT3G13850	33.735	83	55	0	11	93	57	139	1.06e-13	65.9
MsG0580024848.01.T01	AT3G47870	33.735	83	55	0	11	93	57	139	2.80e-13	65.5
MsG0580024848.01.T01	AT1G72980	38.750	80	49	0	11	90	34	113	9.59e-13	62.8
MsG0580024848.01.T01	AT4G22700	36.585	82	51	1	15	95	29	110	1.79e-11	58.9

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCTGAGGGATCCGGTTTA+TGG	0.239083	5:+10741039	MsG0580024848.01.T01:CDS
GCGGGAACACCACCATTATA+AGG	0.269383	5:-10741237	None:intergenic
AATTCTCTTTACCCTTATAA+TGG	0.295168	5:+10741225	MsG0580024848.01.T01:CDS
AAGGAACTTGCTACTTATAT+TGG	0.327213	5:+10741132	MsG0580024848.01.T01:CDS
CAGCAGCATAATAACCCTTT+TGG	0.373437	5:+10741201	MsG0580024848.01.T01:CDS
TGTACATATGCAAATCTTTG+AGG	0.398921	5:-10740916	None:intergenic
ATGAGGCCGAGGCGCGGCTG+AGG	0.424591	5:+10741025	MsG0580024848.01.T01:CDS
CGGGAACACCACCATTATAA+GGG	0.438607	5:-10741236	None:intergenic
GGTTAAGTCGTTGGCTTATG+AGG	0.447260	5:+10741008	MsG0580024848.01.T01:CDS
AGGATTGTCGGTGGAAAATA+CGG	0.447732	5:-10740896	None:intergenic
CGAGGCGCGGCTGAGGGATC+CGG	0.449579	5:+10741032	MsG0580024848.01.T01:CDS
ATTGGTCCTCAGGCGCTTCA+AGG	0.451437	5:+10741150	MsG0580024848.01.T01:CDS
TGCAAATCTTTGAGGATTGT+CGG	0.455151	5:-10740908	None:intergenic
TGCTACTTATATTGGTCCTC+AGG	0.459742	5:+10741140	MsG0580024848.01.T01:CDS
GGGTAAAGAGAATTACCAAA+AGG	0.483287	5:-10741216	None:intergenic
TGAACTTAATAATGCTAAGA+AGG	0.486169	5:+10741113	MsG0580024848.01.T01:CDS
GATCCGGTTTATGGTTGTGT+TGG	0.495699	5:+10741048	MsG0580024848.01.T01:CDS
AGACCAACACAACCATAAAC+CGG	0.496772	5:-10741051	None:intergenic
GGTAAAGAGAATTACCAAAA+GGG	0.512111	5:-10741215	None:intergenic
CATAAGCTAAGAGCTATTCA+AGG	0.519225	5:+10741090	MsG0580024848.01.T01:CDS
TGATGACGCGGTTAAGTCGT+TGG	0.519238	5:+10740999	MsG0580024848.01.T01:CDS
CGGATCCCTCAGCCGCGCCT+CGG	0.522353	5:-10741031	None:intergenic
TGAGGCCGAGGCGCGGCTGA+GGG	0.524798	5:+10741026	MsG0580024848.01.T01:CDS
TGTTGCTGAAGAATCGTTGT+GGG	0.541846	5:-10741177	None:intergenic
TCTCTTTACCCTTATAATGG+TGG	0.546514	5:+10741228	MsG0580024848.01.T01:CDS
GTCGTTGGCTTATGAGGCCG+AGG	0.551333	5:+10741014	MsG0580024848.01.T01:CDS
TTTGTGCACCCGCGGTTACG+CGG	0.557090	5:-10741256	None:intergenic
CGCGACGCAGCGTGATGACG+CGG	0.567241	5:+10740987	MsG0580024848.01.T01:CDS
TGGCTTATGAGGCCGAGGCG+CGG	0.601042	5:+10741019	MsG0580024848.01.T01:CDS
CTGTTGCTGAAGAATCGTTG+TGG	0.605192	5:-10741178	None:intergenic
GGAATACCTTGAAGCGCCTG+AGG	0.633316	5:-10741156	None:intergenic
AAATCTTTGAGGATTGTCGG+TGG	0.714579	5:-10740905	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATTCTCTTTACCCTTATAA+TGG	+	Chr5:10741225-10741244	MsG0580024848.01.T01:CDS	25.0%
!	TGAACTTAATAATGCTAAGA+AGG	+	Chr5:10741113-10741132	MsG0580024848.01.T01:CDS	25.0%
	GGTAAAGAGAATTACCAAAA+GGG	-	Chr5:10741218-10741237	None:intergenic	30.0%
	TGTACATATGCAAATCTTTG+AGG	-	Chr5:10740919-10740938	None:intergenic	30.0%
!	GCATATGTACACAAAGTTTT+TGG	+	Chr5:10740928-10740947	MsG0580024848.01.T01:CDS	30.0%
!!	AAGGAACTTGCTACTTATAT+TGG	+	Chr5:10741132-10741151	MsG0580024848.01.T01:CDS	30.0%
	CATAAGCTAAGAGCTATTCA+AGG	+	Chr5:10741090-10741109	MsG0580024848.01.T01:CDS	35.0%
	GGGTAAAGAGAATTACCAAA+AGG	-	Chr5:10741219-10741238	None:intergenic	35.0%
	TCTCTTTACCCTTATAATGG+TGG	+	Chr5:10741228-10741247	MsG0580024848.01.T01:CDS	35.0%
!	TGCAAATCTTTGAGGATTGT+CGG	-	Chr5:10740911-10740930	None:intergenic	35.0%
	AGACCAACACAACCATAAAC+CGG	-	Chr5:10741054-10741073	None:intergenic	40.0%
	AGGATTGTCGGTGGAAAATA+CGG	-	Chr5:10740899-10740918	None:intergenic	40.0%
	TGTTGCTGAAGAATCGTTGT+GGG	-	Chr5:10741180-10741199	None:intergenic	40.0%
!	AAATCTTTGAGGATTGTCGG+TGG	-	Chr5:10740908-10740927	None:intergenic	40.0%
!	CAGCAGCATAATAACCCTTT+TGG	+	Chr5:10741201-10741220	MsG0580024848.01.T01:CDS	40.0%
!	TGCTACTTATATTGGTCCTC+AGG	+	Chr5:10741140-10741159	MsG0580024848.01.T01:CDS	40.0%
!!!	AGTTTTTGGAGCTAGTAACG+TGG	+	Chr5:10740942-10740961	MsG0580024848.01.T01:CDS	40.0%
	CGGGAACACCACCATTATAA+GGG	-	Chr5:10741239-10741258	None:intergenic	45.0%
	CTGTTGCTGAAGAATCGTTG+TGG	-	Chr5:10741181-10741200	None:intergenic	45.0%
	GATCCGGTTTATGGTTGTGT+TGG	+	Chr5:10741048-10741067	MsG0580024848.01.T01:CDS	45.0%
!!	GGTTAAGTCGTTGGCTTATG+AGG	+	Chr5:10741008-10741027	MsG0580024848.01.T01:CDS	45.0%
	GCGGGAACACCACCATTATA+AGG	-	Chr5:10741240-10741259	None:intergenic	50.0%
!	TGATGACGCGGTTAAGTCGT+TGG	+	Chr5:10740999-10741018	MsG0580024848.01.T01:CDS	50.0%
	ATTGGTCCTCAGGCGCTTCA+AGG	+	Chr5:10741150-10741169	MsG0580024848.01.T01:CDS	55.0%
!	GGAATACCTTGAAGCGCCTG+AGG	-	Chr5:10741159-10741178	None:intergenic	55.0%
	CGGCTGAGGGATCCGGTTTA+TGG	+	Chr5:10741039-10741058	MsG0580024848.01.T01:CDS	60.0%
!!	GTCGTTGGCTTATGAGGCCG+AGG	+	Chr5:10741014-10741033	MsG0580024848.01.T01:CDS	60.0%
	TGGCTTATGAGGCCGAGGCG+CGG	+	Chr5:10741019-10741038	MsG0580024848.01.T01:CDS	65.0%
!	CGCGACGCAGCGTGATGACG+CGG	+	Chr5:10740987-10741006	MsG0580024848.01.T01:CDS	70.0%
	ATGAGGCCGAGGCGCGGCTG+AGG	+	Chr5:10741025-10741044	MsG0580024848.01.T01:CDS	75.0%
	CGAGGCGCGGCTGAGGGATC+CGG	+	Chr5:10741032-10741051	MsG0580024848.01.T01:CDS	75.0%
	CGGATCCCTCAGCCGCGCCT+CGG	-	Chr5:10741034-10741053	None:intergenic	75.0%
	TGAGGCCGAGGCGCGGCTGA+GGG	+	Chr5:10741026-10741045	MsG0580024848.01.T01:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	10740871	10741263	10740871	ID=MsG0580024848.01;Name=MsG0580024848.01
Chr5	mRNA	10740871	10741263	10740871	ID=MsG0580024848.01.T01;Parent=MsG0580024848.01;Name=MsG0580024848.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|130
Chr5	exon	10740871	10741263	10740871	ID=MsG0580024848.01.T01:exon:26548;Parent=MsG0580024848.01.T01
Chr5	CDS	10740871	10741263	10740871	ID=MsG0580024848.01.T01:cds;Parent=MsG0580024848.01.T01

Gene Sequence

>MsG0580024848.01.T01

ATGCACTCAAGAGTGTGTTTTTGCACCGTATTTTCCACCGACAATCCTCAAAGATTTGCATATGTACACAAAGTTTTTGGAGCTAGTAACGTGGCTAAGCTGTTAAACGAGCTTAACGCGACGCAGCGTGATGACGCGGTTAAGTCGTTGGCTTATGAGGCCGAGGCGCGGCTGAGGGATCCGGTTTATGGTTGTGTTGGTCTTATTTCACTTCTTCAGCATAAGCTAAGAGCTATTCAAGGTGAACTTAATAATGCTAAGAAGGAACTTGCTACTTATATTGGTCCTCAGGCGCTTCAAGGTATTCCCACAACGATTCTTCAGCAACAGCAGCAGCATAATAACCCTTTTGGTAATTCTCTTTACCCTTATAATGGTGGTGTTCCCGCGTAA

Protein sequence

>MsG0580024848.01.T01

MHSRVCFCTVFSTDNPQRFAYVHKVFGASNVAKLLNELNATQRDDAVKSLAYEAEARLRDPVYGCVGLISLLQHKLRAIQGELNNAKKELATYIGPQALQGIPTTILQQQQQHNNPFGNSLYPYNGGVPA*