AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025146.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025146.01.T01 MTR_5g022990 98.413 126 2 0 1 126 1 126 3.04e-92 262
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025146.01.T01 AT2G17705 70.866 127 36 1 1 126 1 127 1.59e-68 202

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCGAAACTATCAATTAT+TGG 0.195550 5:-14383126 MsG0580025146.01.T01:CDS
CTTCGAAACTATCAATTATT+GGG 0.214122 5:-14383125 MsG0580025146.01.T01:CDS
ACTGACCAGGAGTATTAATA+AGG 0.288922 5:+14383023 None:intergenic
TGTAGGAGGTGTTGAGGTTG+AGG 0.319767 5:+14383257 None:intergenic
TACTCCTGGTCAGTTTCATA+TGG 0.337358 5:-14383014 MsG0580025146.01.T01:CDS
AGAAGTGATTCGAAGTGCTT+TGG 0.365272 5:+14382946 None:intergenic
AATAACTGCAATCATCTTGT+TGG 0.397222 5:-14383070 MsG0580025146.01.T01:CDS
TAATTGATAGTTTCGAAGAA+TGG 0.420297 5:+14383130 None:intergenic
TTCCTAGAGCTCCTAGGTAT+AGG 0.421841 5:-14382976 MsG0580025146.01.T01:CDS
CCAGCTTCGAAGTAGAAATC+TGG 0.434372 5:+14383226 None:intergenic
CTGACCAGGAGTATTAATAA+GGG 0.458720 5:+14383024 None:intergenic
CCAGATTTCTACTTCGAAGC+TGG 0.468043 5:-14383226 MsG0580025146.01.T01:CDS
GTTTGTGTTGCATTCTGAGC+AGG 0.481040 5:+14383279 None:intergenic
GTTGGTTATGTATATGATGA+TGG 0.481670 5:-14383052 MsG0580025146.01.T01:CDS
ATCTGGTGGGTAGGTGTAGG+AGG 0.483383 5:+14383243 None:intergenic
TTTGAACTTGGTGGCGTCGA+TGG 0.485930 5:+14383177 None:intergenic
GGTAGGTGTAGGAGGTGTTG+AGG 0.497371 5:+14383251 None:intergenic
CCTCCCCTTATTAATACTCC+TGG 0.516089 5:-14383028 MsG0580025146.01.T01:CDS
AAGTTATTCCTAGAGCTCCT+AGG 0.524887 5:-14382982 MsG0580025146.01.T01:CDS
TTCGAAACTATCAATTATTG+GGG 0.530477 5:-14383124 MsG0580025146.01.T01:CDS
GAAATCTGGTGGGTAGGTGT+AGG 0.533007 5:+14383240 None:intergenic
ACTCCTGGTCAGTTTCATAT+GGG 0.544561 5:-14383013 MsG0580025146.01.T01:CDS
AACCTATACCTAGGAGCTCT+AGG 0.549084 5:+14382974 None:intergenic
TTGGATTTAAACCTATACCT+AGG 0.553801 5:+14382965 None:intergenic
GAAGTAGAAATCTGGTGGGT+AGG 0.564551 5:+14383234 None:intergenic
GCTCTAGGAATAACTTGACT+AGG 0.583771 5:+14382989 None:intergenic
TGATAGTTTCGAAGAATGGA+CGG 0.586515 5:+14383134 None:intergenic
GGTGGCGTCGATGGTTGAGA+AGG 0.591033 5:+14383186 None:intergenic
GCTTCGAAGTAGAAATCTGG+TGG 0.607437 5:+14383229 None:intergenic
TGACCAGGAGTATTAATAAG+GGG 0.638197 5:+14383025 None:intergenic
GGACCCATATGAAACTGACC+AGG 0.656975 5:+14383010 None:intergenic
CCAGGAGTATTAATAAGGGG+AGG 0.686307 5:+14383028 None:intergenic
CTTCGAAGTAGAAATCTGGT+GGG 0.688513 5:+14383230 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTCGAAACTATCAATTATT+GGG - Chr5:14383107-14383126 MsG0580025146.01.T01:CDS 25.0%
! TCTTCGAAACTATCAATTAT+TGG - Chr5:14383106-14383125 MsG0580025146.01.T01:CDS 25.0%
! TTCGAAACTATCAATTATTG+GGG - Chr5:14383108-14383127 MsG0580025146.01.T01:CDS 25.0%
!! TAATTGATAGTTTCGAAGAA+TGG + Chr5:14383105-14383124 None:intergenic 25.0%
!!! TTCTTTTTCGAATTTGAACT+TGG + Chr5:14383070-14383089 None:intergenic 25.0%
AATAACTGCAATCATCTTGT+TGG - Chr5:14383162-14383181 MsG0580025146.01.T01:CDS 30.0%
GTTGGTTATGTATATGATGA+TGG - Chr5:14383180-14383199 MsG0580025146.01.T01:CDS 30.0%
TTGGATTTAAACCTATACCT+AGG + Chr5:14383270-14383289 None:intergenic 30.0%
!! TTTTTCGAATTTGAACTTGG+TGG + Chr5:14383067-14383086 None:intergenic 30.0%
TGATAGTTTCGAAGAATGGA+CGG + Chr5:14383101-14383120 None:intergenic 35.0%
!! ACTGACCAGGAGTATTAATA+AGG + Chr5:14383212-14383231 None:intergenic 35.0%
!! CTGACCAGGAGTATTAATAA+GGG + Chr5:14383211-14383230 None:intergenic 35.0%
!! TGACCAGGAGTATTAATAAG+GGG + Chr5:14383210-14383229 None:intergenic 35.0%
AAGTTATTCCTAGAGCTCCT+AGG - Chr5:14383250-14383269 MsG0580025146.01.T01:CDS 40.0%
ACTCCTGGTCAGTTTCATAT+GGG - Chr5:14383219-14383238 MsG0580025146.01.T01:CDS 40.0%
CTTCGAAGTAGAAATCTGGT+GGG + Chr5:14383005-14383024 None:intergenic 40.0%
TACTCCTGGTCAGTTTCATA+TGG - Chr5:14383218-14383237 MsG0580025146.01.T01:CDS 40.0%
! AGAAGTGATTCGAAGTGCTT+TGG + Chr5:14383289-14383308 None:intergenic 40.0%
!! GCTCTAGGAATAACTTGACT+AGG + Chr5:14383246-14383265 None:intergenic 40.0%
AACCTATACCTAGGAGCTCT+AGG + Chr5:14383261-14383280 None:intergenic 45.0%
CCAGATTTCTACTTCGAAGC+TGG - Chr5:14383006-14383025 MsG0580025146.01.T01:CDS 45.0%
CCAGCTTCGAAGTAGAAATC+TGG + Chr5:14383009-14383028 None:intergenic 45.0%
CCTCCCCTTATTAATACTCC+TGG - Chr5:14383204-14383223 MsG0580025146.01.T01:CDS 45.0%
GCTTCGAAGTAGAAATCTGG+TGG + Chr5:14383006-14383025 None:intergenic 45.0%
GTTTGTGTTGCATTCTGAGC+AGG + Chr5:14382956-14382975 None:intergenic 45.0%
TTCCTAGAGCTCCTAGGTAT+AGG - Chr5:14383256-14383275 MsG0580025146.01.T01:CDS 45.0%
! GAAGTAGAAATCTGGTGGGT+AGG + Chr5:14383001-14383020 None:intergenic 45.0%
!! CCAGGAGTATTAATAAGGGG+AGG + Chr5:14383207-14383226 None:intergenic 45.0%
GGACCCATATGAAACTGACC+AGG + Chr5:14383225-14383244 None:intergenic 50.0%
! GAAATCTGGTGGGTAGGTGT+AGG + Chr5:14382995-14383014 None:intergenic 50.0%
!! TGTAGGAGGTGTTGAGGTTG+AGG + Chr5:14382978-14382997 None:intergenic 50.0%
!! TTTGAACTTGGTGGCGTCGA+TGG + Chr5:14383058-14383077 None:intergenic 50.0%
! ATCTGGTGGGTAGGTGTAGG+AGG + Chr5:14382992-14383011 None:intergenic 55.0%
!! GGTAGGTGTAGGAGGTGTTG+AGG + Chr5:14382984-14383003 None:intergenic 55.0%
!! GGTGGCGTCGATGGTTGAGA+AGG + Chr5:14383049-14383068 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 14382937 14383317 14382937 ID=MsG0580025146.01;Name=MsG0580025146.01
Chr5 mRNA 14382937 14383317 14382937 ID=MsG0580025146.01.T01;Parent=MsG0580025146.01;Name=MsG0580025146.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|126
Chr5 exon 14382937 14383317 14382937 ID=MsG0580025146.01.T01:exon:5829;Parent=MsG0580025146.01.T01
Chr5 CDS 14382937 14383317 14382937 ID=MsG0580025146.01.T01:cds;Parent=MsG0580025146.01.T01
Gene Sequence

>MsG0580025146.01.T01

ATGGCTTCAATCTACACCTGCTCAGAATGCAACACAAACCTCAACCTCAACACCTCCTACACCTACCCACCAGATTTCTACTTCGAAGCTGGAAACAAAAACTCAATTTCCTTCTCAACCATCGACGCCACCAAGTTCAAATTCGAAAAAGAAGATAAGATCCGTCCATTCTTCGAAACTATCAATTATTGGGGAATTCAGAGAAAGAGAACGAAGATCAAGTGTAATAACTGCAATCATCTTGTTGGTTATGTATATGATGATGGTCCTCCCCTTATTAATACTCCTGGTCAGTTTCATATGGGTCCTAGTCAAGTTATTCCTAGAGCTCCTAGGTATAGGTTTAAATCCAAAGCACTTCGAATCACTTCTGAAACTTAA
Protein sequence

>MsG0580025146.01.T01

MASIYTCSECNTNLNLNTSYTYPPDFYFEAGNKNSISFSTIDATKFKFEKEDKIRPFFETINYWGIQRKRTKIKCNNCNHLVGYVYDDGPPLINTPGQFHMGPSQVIPRAPRYRFKSKALRITSET*