Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003992.01.T01 | RHN80197.1 | 96.296 | 432 | 16 | 0 | 3 | 434 | 655 | 1086 | 0 | 849 |
| MsG0180003992.01.T01 | RHN80197.1 | 87.943 | 423 | 48 | 3 | 4 | 425 | 5 | 425 | 0 | 747 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003992.01.T01 | P0DO21 | 67.991 | 428 | 128 | 5 | 13 | 434 | 15 | 439 | 0 | 580 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003992.01.T01 | A0A396K0C6 | 96.296 | 432 | 16 | 0 | 3 | 434 | 655 | 1086 | 0.0 | 849 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000148.01 | MsG0180003992.01 | 0.815976 | 7.014037e-52 | 4.907618e-49 |
| MsG0180000491.01 | MsG0180003992.01 | -0.809342 | 1.983869e-50 | 1.161863e-47 |
| MsG0180003992.01 | MsG0180005636.01 | 0.811488 | 6.827109e-51 | 4.231762e-48 |
| MsG0180003992.01 | MsG0180005648.01 | 0.814469 | 1.516574e-51 | 1.018513e-48 |
| MsG0180003992.01 | MsG0180006239.01 | -0.804256 | 2.356712e-49 | 1.209405e-46 |
| MsG0180003992.01 | MsG0280006417.01 | 0.822263 | 2.600535e-53 | 2.165854e-50 |
| MsG0180003992.01 | MsG0280007047.01 | -0.805113 | 1.561741e-49 | 8.193624e-47 |
| MsG0180003992.01 | MsG0280007201.01 | 0.823372 | 1.435317e-53 | 1.233431e-50 |
| MsG0180003992.01 | MsG0280007418.01 | -0.814070 | 1.857594e-51 | 1.234076e-48 |
| MsG0180003992.01 | MsG0280008531.01 | -0.811329 | 7.393877e-51 | 4.563415e-48 |
| MsG0180003992.01 | MsG0280009434.01 | -0.802215 | 6.236122e-49 | 3.037372e-46 |
| MsG0180003992.01 | MsG0380015369.01 | 0.814543 | 1.460610e-51 | 9.828503e-49 |
| MsG0180003992.01 | MsG0380016086.01 | 0.815685 | 8.145948e-52 | 5.654515e-49 |
| MsG0180003992.01 | MsG0380016176.01 | 0.804011 | 2.650989e-49 | 1.351960e-46 |
| MsG0180003992.01 | MsG0380016530.01 | -0.807617 | 4.631956e-50 | 2.591834e-47 |
| MsG0180003992.01 | MsG0380017859.01 | 0.829156 | 6.026557e-55 | 6.117255e-52 |
| MsG0180003992.01 | MsG0480018910.01 | -0.820340 | 7.224415e-53 | 5.700711e-50 |
| MsG0180003992.01 | MsG0480019693.01 | -0.807010 | 6.228431e-50 | 3.430728e-47 |
| MsG0180003992.01 | MsG0480022383.01 | 0.818973 | 1.481807e-52 | 1.125864e-49 |
| MsG0180003992.01 | MsG0480023821.01 | -0.808497 | 3.008496e-50 | 1.722864e-47 |
| MsG0180003992.01 | MsG0580024178.01 | 0.809232 | 2.095255e-50 | 1.223484e-47 |
| MsG0180003992.01 | MsG0580024404.01 | -0.807197 | 5.684705e-50 | 3.146543e-47 |
| MsG0180003992.01 | MsG0580025146.01 | 0.804919 | 1.714356e-49 | 8.950431e-47 |
| MsG0180003992.01 | MsG0580029918.01 | -0.801210 | 1.002699e-48 | 4.761075e-46 |
| MsG0180003992.01 | MsG0680031139.01 | 0.818631 | 1.772165e-52 | 1.333570e-49 |
| MsG0180003992.01 | MsG0680031852.01 | 0.813765 | 2.168402e-51 | 1.428632e-48 |
| MsG0180003992.01 | MsG0680032167.01 | 0.838686 | 2.480950e-57 | 3.348298e-54 |
| MsG0180003992.01 | MsG0680034980.01 | 0.816499 | 5.359390e-52 | 3.803731e-49 |
| MsG0180003992.01 | MsG0780041510.01 | 0.844195 | 8.785894e-59 | 1.407775e-55 |
| MsG0180003992.01 | MsG0780041612.01 | 0.810783 | 9.706938e-51 | 5.905570e-48 |
| MsG0180003992.01 | MsG0880046540.01 | -0.808216 | 3.453387e-50 | 1.963155e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003992.01.T01 | MTR_1g072420 | 96.296 | 432 | 16 | 0 | 3 | 434 | 4 | 435 | 0.0 | 848 |
| MsG0180003992.01.T01 | MTR_1g072450 | 88.194 | 432 | 48 | 3 | 4 | 434 | 5 | 434 | 0.0 | 764 |
| MsG0180003992.01.T01 | MTR_1g072380 | 79.710 | 414 | 74 | 4 | 29 | 434 | 40 | 451 | 0.0 | 654 |
| MsG0180003992.01.T01 | MTR_2g022450 | 54.779 | 429 | 184 | 6 | 10 | 433 | 6 | 429 | 2.09e-161 | 462 |
| MsG0180003992.01.T01 | MTR_1g093100 | 53.761 | 452 | 173 | 8 | 1 | 433 | 1 | 435 | 1.79e-159 | 457 |
| MsG0180003992.01.T01 | MTR_4g117090 | 46.698 | 424 | 198 | 10 | 28 | 428 | 33 | 451 | 2.32e-114 | 343 |
| MsG0180003992.01.T01 | MTR_7g113790 | 41.441 | 444 | 222 | 13 | 8 | 432 | 11 | 435 | 1.72e-101 | 309 |
| MsG0180003992.01.T01 | MTR_2g022430 | 42.664 | 443 | 214 | 12 | 1 | 426 | 1 | 420 | 8.51e-98 | 299 |
| MsG0180003992.01.T01 | MTR_2g022380 | 42.889 | 443 | 219 | 10 | 1 | 429 | 1 | 423 | 6.60e-94 | 290 |
| MsG0180003992.01.T01 | MTR_2g022370 | 42.597 | 439 | 222 | 10 | 1 | 429 | 1 | 419 | 6.60e-94 | 290 |
| MsG0180003992.01.T01 | MTR_2g022350 | 42.534 | 442 | 216 | 11 | 1 | 426 | 1 | 420 | 1.01e-91 | 284 |
| MsG0180003992.01.T01 | MTR_2g022460 | 41.748 | 412 | 204 | 10 | 31 | 432 | 26 | 411 | 1.12e-91 | 283 |
| MsG0180003992.01.T01 | MTR_2g022320 | 42.548 | 416 | 197 | 10 | 32 | 427 | 24 | 417 | 2.81e-89 | 277 |
| MsG0180003992.01.T01 | MTR_2g022490 | 42.145 | 401 | 187 | 10 | 28 | 406 | 27 | 404 | 3.42e-88 | 275 |
| MsG0180003992.01.T01 | MTR_2g022480 | 39.952 | 418 | 220 | 11 | 5 | 406 | 8 | 410 | 9.71e-87 | 271 |
| MsG0180003992.01.T01 | MTR_0252s0030 | 43.779 | 217 | 101 | 5 | 190 | 395 | 140 | 346 | 2.32e-44 | 158 |
| MsG0180003992.01.T01 | MTR_1g093130 | 44.578 | 166 | 72 | 5 | 230 | 394 | 6 | 152 | 2.67e-30 | 115 |
| MsG0180003992.01.T01 | MTR_7g062360 | 22.762 | 391 | 240 | 15 | 54 | 417 | 68 | 423 | 6.15e-19 | 89.0 |
| MsG0180003992.01.T01 | MTR_7g105850 | 26.034 | 411 | 221 | 19 | 46 | 414 | 91 | 460 | 1.65e-17 | 84.7 |
| MsG0180003992.01.T01 | MTR_3g046300 | 24.422 | 389 | 231 | 15 | 54 | 417 | 106 | 456 | 2.08e-16 | 81.6 |
| MsG0180003992.01.T01 | MTR_3g097150 | 23.529 | 391 | 235 | 17 | 41 | 414 | 98 | 441 | 5.28e-15 | 77.0 |
| MsG0180003992.01.T01 | MTR_3g068000 | 23.077 | 390 | 228 | 17 | 59 | 415 | 87 | 437 | 1.50e-14 | 75.9 |
| MsG0180003992.01.T01 | MTR_4g100990 | 24.510 | 306 | 179 | 9 | 134 | 415 | 189 | 466 | 2.62e-14 | 75.1 |
| MsG0180003992.01.T01 | MTR_5g091130 | 22.938 | 388 | 239 | 16 | 54 | 417 | 200 | 551 | 3.71e-14 | 74.7 |
| MsG0180003992.01.T01 | MTR_2g022330 | 63.462 | 52 | 19 | 0 | 377 | 428 | 34 | 85 | 6.47e-14 | 67.4 |
| MsG0180003992.01.T01 | MTR_3g028120 | 23.560 | 382 | 223 | 16 | 54 | 414 | 98 | 431 | 3.21e-13 | 71.6 |
| MsG0180003992.01.T01 | MTR_1g111350 | 25.000 | 412 | 230 | 18 | 25 | 415 | 110 | 463 | 4.34e-12 | 68.2 |
| MsG0180003992.01.T01 | MTR_8g103880 | 23.834 | 386 | 223 | 16 | 43 | 410 | 114 | 446 | 2.39e-11 | 65.9 |
| MsG0180003992.01.T01 | MTR_2g055840 | 24.219 | 384 | 227 | 16 | 54 | 412 | 22 | 366 | 4.03e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003992.01.T01 | AT1G03220 | 64.486 | 428 | 146 | 4 | 10 | 434 | 9 | 433 | 0.0 | 548 |
| MsG0180003992.01.T01 | AT1G03230 | 62.651 | 415 | 149 | 4 | 23 | 434 | 23 | 434 | 0.0 | 516 |
| MsG0180003992.01.T01 | AT5G19120 | 36.562 | 413 | 206 | 10 | 12 | 414 | 9 | 375 | 1.26e-60 | 202 |
| MsG0180003992.01.T01 | AT5G19110 | 31.221 | 426 | 258 | 8 | 9 | 428 | 5 | 401 | 1.63e-55 | 189 |
| MsG0180003992.01.T01 | AT5G19110 | 31.221 | 426 | 258 | 8 | 9 | 428 | 41 | 437 | 3.63e-55 | 189 |
| MsG0180003992.01.T01 | AT5G48430 | 32.009 | 428 | 246 | 16 | 13 | 429 | 10 | 403 | 4.33e-48 | 169 |
| MsG0180003992.01.T01 | AT5G19100 | 35.372 | 376 | 183 | 14 | 62 | 426 | 58 | 384 | 5.03e-46 | 164 |
| MsG0180003992.01.T01 | AT2G39710 | 26.440 | 382 | 223 | 17 | 59 | 415 | 78 | 426 | 5.31e-21 | 95.1 |
| MsG0180003992.01.T01 | AT3G25700 | 25.714 | 420 | 227 | 20 | 35 | 415 | 72 | 445 | 4.83e-19 | 89.4 |
| MsG0180003992.01.T01 | AT5G02190 | 22.251 | 391 | 232 | 16 | 59 | 415 | 86 | 438 | 1.71e-14 | 75.5 |
| MsG0180003992.01.T01 | AT3G52500 | 21.814 | 408 | 247 | 16 | 46 | 415 | 90 | 463 | 6.24e-12 | 67.8 |
| MsG0180003992.01.T01 | AT1G09750 | 24.088 | 411 | 238 | 17 | 28 | 415 | 85 | 444 | 9.10e-11 | 63.9 |
Find 109 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCTCTCAACAATCTTTC+AGG | 0.152886 | 1:-71479627 | MsG0180003992.01.T01:CDS |
| TGATTTGGCTGCTTCTAAAT+TGG | 0.163227 | 1:-71478474 | MsG0180003992.01.T01:CDS |
| TTGAGATGGAAGAGCGATTT+TGG | 0.217986 | 1:+71479138 | None:intergenic |
| GCACCAACTTTCTTACTAAA+AGG | 0.223724 | 1:-71479202 | MsG0180003992.01.T01:CDS |
| ATCTCGGTGGCCAATTCCTC+TGG | 0.231618 | 1:-71479498 | MsG0180003992.01.T01:CDS |
| TGTAATGAAGTATTGAAAAT+TGG | 0.259063 | 1:+71479681 | None:intergenic |
| CGCAATCAACCCAGAGGAAT+TGG | 0.261348 | 1:+71479488 | None:intergenic |
| GGATTTAGCTCCTTGCTCTT+TGG | 0.298601 | 1:-71478452 | MsG0180003992.01.T01:CDS |
| TTATCAATCCAATCCTCTAA+TGG | 0.328408 | 1:-71479274 | MsG0180003992.01.T01:CDS |
| AATGTGGTTTGGAGGATATT+TGG | 0.337063 | 1:-71478620 | MsG0180003992.01.T01:CDS |
| TAATCTTGTGCAATTTGATT+TGG | 0.358886 | 1:-71478489 | MsG0180003992.01.T01:CDS |
| GGTTGATTTGAGCTTTGTAT+TGG | 0.360990 | 1:+71479557 | None:intergenic |
| ACTGGGAGAACTAGGGCTTT+TGG | 0.362155 | 1:+71479604 | None:intergenic |
| GATTTGGCTGCTTCTAAATT+GGG | 0.364199 | 1:-71478473 | MsG0180003992.01.T01:CDS |
| TCCTGGGTTGAAGCCATTAG+AGG | 0.369624 | 1:+71479261 | None:intergenic |
| TGCAAAATGAAAATGTGGTT+TGG | 0.372624 | 1:-71478631 | MsG0180003992.01.T01:CDS |
| GGACTTGCAAGTGGTGCTTC+TGG | 0.379627 | 1:-71479181 | MsG0180003992.01.T01:CDS |
| TTGTGTCTTGGATTTGTGAA+TGG | 0.380483 | 1:-71478563 | MsG0180003992.01.T01:CDS |
| ATTTGTCTTTCCTCTTCAAA+TGG | 0.384499 | 1:-71479079 | MsG0180003992.01.T01:CDS |
| GTGGGACTAGTGGAGTTCTT+TGG | 0.385628 | 1:+71479536 | None:intergenic |
| AAAGCAGAGGCTGTGCTAAC+AGG | 0.388954 | 1:+71478965 | None:intergenic |
| GCTTCTGGTATGGCTGGTCT+TGG | 0.413917 | 1:-71479166 | MsG0180003992.01.T01:CDS |
| GAAGCTTCTAAAACTGTGTA+AGG | 0.415913 | 1:+71478803 | None:intergenic |
| CAGGTGTTGTTGTTGCAGCC+AGG | 0.418057 | 1:+71479361 | None:intergenic |
| ACAAGGACTTCAATTGTGAT+TGG | 0.421714 | 1:-71478530 | MsG0180003992.01.T01:CDS |
| AAACATAAAGAGGGTGGATT+CGG | 0.425811 | 1:-71478732 | MsG0180003992.01.T01:CDS |
| TCTTCCATCTCAATTAGCTT+CGG | 0.430182 | 1:-71479128 | MsG0180003992.01.T01:CDS |
| TCTGAATCTAAAACAATGTT+GGG | 0.433075 | 1:+71479019 | None:intergenic |
| TTCCCCGCTGGTGGCGGTGT+GGG | 0.440917 | 1:+71479312 | None:intergenic |
| TGTGGGTGATACTATTGTCC+GGG | 0.441956 | 1:+71479329 | None:intergenic |
| AGCAAGTTCCCCGCTGGTGG+CGG | 0.445918 | 1:+71479306 | None:intergenic |
| GATATTTGGTGCAAATTCAA+TGG | 0.448768 | 1:-71478606 | MsG0180003992.01.T01:CDS |
| TTGTTGTTGCAGCCAGGCTT+TGG | 0.449409 | 1:+71479367 | None:intergenic |
| CTCGCCAACTCCGACGGCTG+TGG | 0.450635 | 1:-71479406 | MsG0180003992.01.T01:CDS |
| GGTTGAAGCCATTAGAGGAT+TGG | 0.451183 | 1:+71479266 | None:intergenic |
| ATCTGAATCTAAAACAATGT+TGG | 0.455505 | 1:+71479018 | None:intergenic |
| GAAACTGCGACATTTCGTCC+TGG | 0.459544 | 1:+71479244 | None:intergenic |
| AGTGGTGCTTCTGGTATGGC+TGG | 0.465972 | 1:-71479172 | MsG0180003992.01.T01:CDS |
| TGCAAGTGGTGCTTCTGGTA+TGG | 0.475769 | 1:-71479176 | MsG0180003992.01.T01:CDS |
| TGAGCGGAGCGACACCGTGC+GGG | 0.482353 | 1:+71479436 | None:intergenic |
| ACACCGTGCGGGGCGGTATG+TGG | 0.483374 | 1:+71479447 | None:intergenic |
| GTGTGGGTGATACTATTGTC+CGG | 0.486838 | 1:+71479328 | None:intergenic |
| TTATTCGGGGATGGACCTTA+CGG | 0.487213 | 1:-71479049 | MsG0180003992.01.T01:CDS |
| TGTTTCTCATCACCAAAGCC+TGG | 0.494972 | 1:-71479379 | MsG0180003992.01.T01:CDS |
| TCCTCTAATGGCTTCAACCC+AGG | 0.495018 | 1:-71479262 | MsG0180003992.01.T01:CDS |
| CTCAACGTCATCGTCGATCT+CGG | 0.497477 | 1:-71479514 | MsG0180003992.01.T01:CDS |
| AAACTGCGACATTTCGTCCT+GGG | 0.498880 | 1:+71479245 | None:intergenic |
| AAGTTGGTGCACATGAGAAT+AGG | 0.499306 | 1:+71479215 | None:intergenic |
| TCACCCACACCGCCACCAGC+GGG | 0.508942 | 1:-71479315 | MsG0180003992.01.T01:CDS |
| TCTCGGTGGCCAATTCCTCT+GGG | 0.509201 | 1:-71479497 | MsG0180003992.01.T01:CDS |
| TGTCTTGGATTTGTGAATGG+TGG | 0.517202 | 1:-71478560 | MsG0180003992.01.T01:CDS |
| GATTGGAGGGTATCAGTTGG+AGG | 0.520806 | 1:-71478513 | MsG0180003992.01.T01:CDS |
| TTAGTGTAACAAAATTCAAA+AGG | 0.525717 | 1:+71478704 | None:intergenic |
| TTCAGCAAGTTCCCCGCTGG+TGG | 0.526258 | 1:+71479303 | None:intergenic |
| ATCACCACAGCCGTCGGAGT+TGG | 0.528866 | 1:+71479402 | None:intergenic |
| GAAAGATTGTTGAGAGAAAG+TGG | 0.530349 | 1:+71479630 | None:intergenic |
| AATGTGACTGGGACACGATT+GGG | 0.533667 | 1:-71478683 | MsG0180003992.01.T01:CDS |
| TAATGTGACTGGGACACGAT+TGG | 0.534463 | 1:-71478684 | MsG0180003992.01.T01:CDS |
| GTTCTCCCAGTCACAAAAGA+TGG | 0.534725 | 1:-71479592 | MsG0180003992.01.T01:CDS |
| CACAGCCGTCGGAGTTGGCG+AGG | 0.537925 | 1:+71479407 | None:intergenic |
| TCTATAGACAATAACGGTGT+TGG | 0.538992 | 1:-71478851 | MsG0180003992.01.T01:CDS |
| AGGACTTCAATTGTGATTGG+AGG | 0.545988 | 1:-71478527 | MsG0180003992.01.T01:CDS |
| ATAGACAATAACGGTGTTGG+TGG | 0.545992 | 1:-71478848 | MsG0180003992.01.T01:CDS |
| ATTGTCCGGGGTGACACCAC+AGG | 0.546863 | 1:+71479342 | None:intergenic |
| GTTCCCCGCTGGTGGCGGTG+TGG | 0.552011 | 1:+71479311 | None:intergenic |
| TGCTCCCTCGCCAACTCCGA+CGG | 0.556363 | 1:-71479412 | MsG0180003992.01.T01:CDS |
| GTATTGAAAATTGGAAGCCA+TGG | 0.560507 | 1:+71479690 | None:intergenic |
| AAAATGAAAATGTGGTTTGG+AGG | 0.560548 | 1:-71478628 | MsG0180003992.01.T01:CDS |
| GGACTTCAATTGTGATTGGA+GGG | 0.563151 | 1:-71478526 | MsG0180003992.01.T01:CDS |
| TAAAACAACTCCATTTGAAG+AGG | 0.563234 | 1:+71479069 | None:intergenic |
| ATCTTCAGCAAGTTCCCCGC+TGG | 0.564390 | 1:+71479300 | None:intergenic |
| TGAATGGTGGTAAGAATACA+AGG | 0.567635 | 1:-71478547 | MsG0180003992.01.T01:CDS |
| AAAGCCGAAGCTAATTGAGA+TGG | 0.567723 | 1:+71479124 | None:intergenic |
| AGATCGACGATGACGTTGAG+TGG | 0.568219 | 1:+71479517 | None:intergenic |
| AATGGTGAATATAAATGATG+AGG | 0.569327 | 1:-71478588 | MsG0180003992.01.T01:CDS |
| CTGCTGCAAGAAACATAAAG+AGG | 0.579468 | 1:-71478742 | MsG0180003992.01.T01:CDS |
| TGTGATTGGAGGGTATCAGT+TGG | 0.585357 | 1:-71478516 | MsG0180003992.01.T01:CDS |
| TTACTAAAAGGACTTGCAAG+TGG | 0.586199 | 1:-71479190 | MsG0180003992.01.T01:CDS |
| CCTTCAGTCGAGTATTTCAT+CGG | 0.588349 | 1:-71478929 | MsG0180003992.01.T01:CDS |
| TTTGTTACACTAATGTGACT+GGG | 0.589924 | 1:-71478694 | MsG0180003992.01.T01:CDS |
| ATGACGTTGAGTGGGACTAG+TGG | 0.593471 | 1:+71479526 | None:intergenic |
| ATCACCCACACCGCCACCAG+CGG | 0.595520 | 1:-71479316 | MsG0180003992.01.T01:CDS |
| GAGCGGAGCGACACCGTGCG+GGG | 0.598071 | 1:+71479437 | None:intergenic |
| ACAGCCTCTGCTTTCTCACA+AGG | 0.600516 | 1:-71478956 | MsG0180003992.01.T01:CDS |
| TTGTTGTCTATAGACAATAA+CGG | 0.601082 | 1:-71478857 | MsG0180003992.01.T01:CDS |
| CCGTGCGGGGCGGTATGTGG+AGG | 0.605839 | 1:+71479450 | None:intergenic |
| CAACACCTGTGGTGTCACCC+CGG | 0.614141 | 1:-71479347 | MsG0180003992.01.T01:CDS |
| CCGATGAAATACTCGACTGA+AGG | 0.615364 | 1:+71478929 | None:intergenic |
| CCTCCACATACCGCCCCGCA+CGG | 0.625778 | 1:-71479450 | MsG0180003992.01.T01:CDS |
| ACAGCCGTCGGAGTTGGCGA+GGG | 0.625946 | 1:+71479408 | None:intergenic |
| TGCTGCAAGAAACATAAAGA+GGG | 0.632185 | 1:-71478741 | MsG0180003992.01.T01:CDS |
| AGTTTGTCGTCCAAAGAGCA+AGG | 0.633294 | 1:+71478442 | None:intergenic |
| TTGAGCGGAGCGACACCGTG+CGG | 0.647661 | 1:+71479435 | None:intergenic |
| GATCGACGATGACGTTGAGT+GGG | 0.647965 | 1:+71479518 | None:intergenic |
| CGGAGCGACACCGTGCGGGG+CGG | 0.655775 | 1:+71479440 | None:intergenic |
| AAAAGGCATCTAGGCAGTGG+AGG | 0.665153 | 1:+71478397 | None:intergenic |
| CTAACAGGATTGATCAAAAG+CGG | 0.665242 | 1:+71478980 | None:intergenic |
| TGCAAGAAACATAAAGAGGG+TGG | 0.665566 | 1:-71478738 | MsG0180003992.01.T01:CDS |
| GTGGGTGATACTATTGTCCG+GGG | 0.667197 | 1:+71479330 | None:intergenic |
| TGGTGGAACGAAGATCAGTA+CGG | 0.669814 | 1:-71478831 | MsG0180003992.01.T01:CDS |
| ATGTTGGGAAGAAAACCGTA+AGG | 0.671605 | 1:+71479034 | None:intergenic |
| CTGACCTTGTGAGAAAGCAG+AGG | 0.675122 | 1:+71478952 | None:intergenic |
| GTTGGCGAGGGAGCATTGAG+CGG | 0.677826 | 1:+71479420 | None:intergenic |
| GAAACAATCACCACAGCCGT+CGG | 0.679467 | 1:+71479396 | None:intergenic |
| CACCCACACCGCCACCAGCG+GGG | 0.684548 | 1:-71479314 | MsG0180003992.01.T01:CDS |
| GGTATGTGGAGGAAATGTAG+TGG | 0.686881 | 1:+71479461 | None:intergenic |
| GGCTGCAACAACAACACCTG+TGG | 0.687479 | 1:-71479358 | MsG0180003992.01.T01:CDS |
| AACGTCATCGTCGATCTCGG+TGG | 0.719707 | 1:-71479511 | MsG0180003992.01.T01:CDS |
| AAAGATTGTTGAGAGAAAGT+GGG | 0.736615 | 1:+71479631 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGTGTAACAAAATTCAAA+AGG | + | Chr1:71479393-71479412 | None:intergenic | 20.0% |
| !!! | TGTAATGAAGTATTGAAAAT+TGG | + | Chr1:71478416-71478435 | None:intergenic | 20.0% |
| ! | AAATGAAGAAAAATAGGAGT+AGG | + | Chr1:71478441-71478460 | None:intergenic | 25.0% |
| ! | AATGAAGAAAAATAGGAGTA+GGG | + | Chr1:71478440-71478459 | None:intergenic | 25.0% |
| ! | AATGGTGAATATAAATGATG+AGG | - | Chr1:71479506-71479525 | MsG0180003992.01.T01:CDS | 25.0% |
| ! | TAATCTTGTGCAATTTGATT+TGG | - | Chr1:71479605-71479624 | MsG0180003992.01.T01:CDS | 25.0% |
| ! | TTGTTGTCTATAGACAATAA+CGG | - | Chr1:71479237-71479256 | MsG0180003992.01.T01:CDS | 25.0% |
| !! | ATCTGAATCTAAAACAATGT+TGG | + | Chr1:71479079-71479098 | None:intergenic | 25.0% |
| !! | CTTTTTGCAAAATGAAAATG+TGG | - | Chr1:71479458-71479477 | MsG0180003992.01.T01:CDS | 25.0% |
| !! | TCTGAATCTAAAACAATGTT+GGG | + | Chr1:71479078-71479097 | None:intergenic | 25.0% |
| !!! | TCAAATGGAGTTGTTTTATT+CGG | - | Chr1:71479030-71479049 | MsG0180003992.01.T01:CDS | 25.0% |
| AAAGATTGTTGAGAGAAAGT+GGG | + | Chr1:71478466-71478485 | None:intergenic | 30.0% | |
| ATTTGTCTTTCCTCTTCAAA+TGG | - | Chr1:71479015-71479034 | MsG0180003992.01.T01:CDS | 30.0% | |
| TAAAACAACTCCATTTGAAG+AGG | + | Chr1:71479028-71479047 | None:intergenic | 30.0% | |
| TGCAAAATGAAAATGTGGTT+TGG | - | Chr1:71479463-71479482 | MsG0180003992.01.T01:CDS | 30.0% | |
| TGGGTGAAATGAAGAAAAAT+AGG | + | Chr1:71478447-71478466 | None:intergenic | 30.0% | |
| TTATCAATCCAATCCTCTAA+TGG | - | Chr1:71478820-71478839 | MsG0180003992.01.T01:CDS | 30.0% | |
| TTTGTTACACTAATGTGACT+GGG | - | Chr1:71479400-71479419 | MsG0180003992.01.T01:CDS | 30.0% | |
| ! | AAAATGAAAATGTGGTTTGG+AGG | - | Chr1:71479466-71479485 | MsG0180003992.01.T01:CDS | 30.0% |
| ! | AGTCCTTTTAGTAAGAAAGT+TGG | + | Chr1:71478898-71478917 | None:intergenic | 30.0% |
| ! | TTTTGTTACACTAATGTGAC+TGG | - | Chr1:71479399-71479418 | MsG0180003992.01.T01:CDS | 30.0% |
| !! | AAATGGAGTTGTTTTATTCG+GGG | - | Chr1:71479032-71479051 | MsG0180003992.01.T01:CDS | 30.0% |
| !! | CAAATGGAGTTGTTTTATTC+GGG | - | Chr1:71479031-71479050 | MsG0180003992.01.T01:CDS | 30.0% |
| !! | GATATTTGGTGCAAATTCAA+TGG | - | Chr1:71479488-71479507 | MsG0180003992.01.T01:CDS | 30.0% |
| ACAAGGACTTCAATTGTGAT+TGG | - | Chr1:71479564-71479583 | MsG0180003992.01.T01:CDS | 35.0% | |
| CTAACAGGATTGATCAAAAG+CGG | + | Chr1:71479117-71479136 | None:intergenic | 35.0% | |
| CTTTCTCTCAACAATCTTTC+AGG | - | Chr1:71478467-71478486 | MsG0180003992.01.T01:CDS | 35.0% | |
| GAAAGATTGTTGAGAGAAAG+TGG | + | Chr1:71478467-71478486 | None:intergenic | 35.0% | |
| GATTTGGCTGCTTCTAAATT+GGG | - | Chr1:71479621-71479640 | MsG0180003992.01.T01:CDS | 35.0% | |
| GCACCAACTTTCTTACTAAA+AGG | - | Chr1:71478892-71478911 | MsG0180003992.01.T01:CDS | 35.0% | |
| TCTATAGACAATAACGGTGT+TGG | - | Chr1:71479243-71479262 | MsG0180003992.01.T01:CDS | 35.0% | |
| TGAATGGTGGTAAGAATACA+AGG | - | Chr1:71479547-71479566 | MsG0180003992.01.T01:CDS | 35.0% | |
| TGCAAAAAGAGCTCAATAGT+AGG | + | Chr1:71479447-71479466 | None:intergenic | 35.0% | |
| TGCTGCAAGAAACATAAAGA+GGG | - | Chr1:71479353-71479372 | MsG0180003992.01.T01:CDS | 35.0% | |
| TTACTAAAAGGACTTGCAAG+TGG | - | Chr1:71478904-71478923 | MsG0180003992.01.T01:CDS | 35.0% | |
| TTGTGTCTTGGATTTGTGAA+TGG | - | Chr1:71479531-71479550 | MsG0180003992.01.T01:CDS | 35.0% | |
| ! | AAACATAAAGAGGGTGGATT+CGG | - | Chr1:71479362-71479381 | MsG0180003992.01.T01:CDS | 35.0% |
| ! | AATGTGGTTTGGAGGATATT+TGG | - | Chr1:71479474-71479493 | MsG0180003992.01.T01:CDS | 35.0% |
| ! | TCTTCCATCTCAATTAGCTT+CGG | - | Chr1:71478966-71478985 | MsG0180003992.01.T01:CDS | 35.0% |
| ! | TGATTTGGCTGCTTCTAAAT+TGG | - | Chr1:71479620-71479639 | MsG0180003992.01.T01:CDS | 35.0% |
| !! | AATGATGAGGTTTTGTGTCT+TGG | - | Chr1:71479519-71479538 | MsG0180003992.01.T01:CDS | 35.0% |
| !! | GAAGCTTCTAAAACTGTGTA+AGG | + | Chr1:71479294-71479313 | None:intergenic | 35.0% |
| !! | GGTTGATTTGAGCTTTGTAT+TGG | + | Chr1:71478540-71478559 | None:intergenic | 35.0% |
| AAAGCCGAAGCTAATTGAGA+TGG | + | Chr1:71478973-71478992 | None:intergenic | 40.0% | |
| AGGACTTCAATTGTGATTGG+AGG | - | Chr1:71479567-71479586 | MsG0180003992.01.T01:CDS | 40.0% | |
| ATGTTGGGAAGAAAACCGTA+AGG | + | Chr1:71479063-71479082 | None:intergenic | 40.0% | |
| CCTTCAGTCGAGTATTTCAT+CGG | - | Chr1:71479165-71479184 | MsG0180003992.01.T01:CDS | 40.0% | |
| CTGCTGCAAGAAACATAAAG+AGG | - | Chr1:71479352-71479371 | MsG0180003992.01.T01:CDS | 40.0% | |
| GGACTTCAATTGTGATTGGA+GGG | - | Chr1:71479568-71479587 | MsG0180003992.01.T01:CDS | 40.0% | |
| TGCAAGAAACATAAAGAGGG+TGG | - | Chr1:71479356-71479375 | MsG0180003992.01.T01:CDS | 40.0% | |
| TGTCTTGGATTTGTGAATGG+TGG | - | Chr1:71479534-71479553 | MsG0180003992.01.T01:CDS | 40.0% | |
| ! | ATAGACAATAACGGTGTTGG+TGG | - | Chr1:71479246-71479265 | MsG0180003992.01.T01:CDS | 40.0% |
| ! | TTGAGATGGAAGAGCGATTT+TGG | + | Chr1:71478959-71478978 | None:intergenic | 40.0% |
| ! | TTTTGTGACTGGGAGAACTA+GGG | + | Chr1:71478500-71478519 | None:intergenic | 40.0% |
| !! | AAGTTGGTGCACATGAGAAT+AGG | + | Chr1:71478882-71478901 | None:intergenic | 40.0% |
| AAACTGCGACATTTCGTCCT+GGG | + | Chr1:71478852-71478871 | None:intergenic | 45.0% | |
| AATGTGACTGGGACACGATT+GGG | - | Chr1:71479411-71479430 | MsG0180003992.01.T01:CDS | 45.0% | |
| AGTTTGTCGTCCAAAGAGCA+AGG | + | Chr1:71479655-71479674 | None:intergenic | 45.0% | |
| CCGATGAAATACTCGACTGA+AGG | + | Chr1:71479168-71479187 | None:intergenic | 45.0% | |
| GGTATGTGGAGGAAATGTAG+TGG | + | Chr1:71478636-71478655 | None:intergenic | 45.0% | |
| GGTTGAAGCCATTAGAGGAT+TGG | + | Chr1:71478831-71478850 | None:intergenic | 45.0% | |
| GTGTGGGTGATACTATTGTC+CGG | + | Chr1:71478769-71478788 | None:intergenic | 45.0% | |
| GTTCTCCCAGTCACAAAAGA+TGG | - | Chr1:71478502-71478521 | MsG0180003992.01.T01:CDS | 45.0% | |
| TAATGTGACTGGGACACGAT+TGG | - | Chr1:71479410-71479429 | MsG0180003992.01.T01:CDS | 45.0% | |
| TGGTGGAACGAAGATCAGTA+CGG | - | Chr1:71479263-71479282 | MsG0180003992.01.T01:CDS | 45.0% | |
| TGTGGGTGATACTATTGTCC+GGG | + | Chr1:71478768-71478787 | None:intergenic | 45.0% | |
| TGTTTCTCATCACCAAAGCC+TGG | - | Chr1:71478715-71478734 | MsG0180003992.01.T01:CDS | 45.0% | |
| TTATTCGGGGATGGACCTTA+CGG | - | Chr1:71479045-71479064 | MsG0180003992.01.T01:CDS | 45.0% | |
| ! | CTTTTGTGACTGGGAGAACT+AGG | + | Chr1:71478501-71478520 | None:intergenic | 45.0% |
| ! | GGATTTAGCTCCTTGCTCTT+TGG | - | Chr1:71479642-71479661 | MsG0180003992.01.T01:CDS | 45.0% |
| ! | TAGCGCCATCTTTTGTGACT+GGG | + | Chr1:71478510-71478529 | None:intergenic | 45.0% |
| !! | GGAGTTGTTTTATTCGGGGA+TGG | - | Chr1:71479036-71479055 | MsG0180003992.01.T01:CDS | 45.0% |
| !! | TGTGATTGGAGGGTATCAGT+TGG | - | Chr1:71479578-71479597 | MsG0180003992.01.T01:CDS | 45.0% |
| AAAGCAGAGGCTGTGCTAAC+AGG | + | Chr1:71479132-71479151 | None:intergenic | 50.0% | |
| ACAGCCTCTGCTTTCTCACA+AGG | - | Chr1:71479138-71479157 | MsG0180003992.01.T01:CDS | 50.0% | |
| AGATCGACGATGACGTTGAG+TGG | + | Chr1:71478580-71478599 | None:intergenic | 50.0% | |
| ATGACGTTGAGTGGGACTAG+TGG | + | Chr1:71478571-71478590 | None:intergenic | 50.0% | |
| CGCAATCAACCCAGAGGAAT+TGG | + | Chr1:71478609-71478628 | None:intergenic | 50.0% | |
| CTCAACGTCATCGTCGATCT+CGG | - | Chr1:71478580-71478599 | MsG0180003992.01.T01:CDS | 50.0% | |
| CTGACCTTGTGAGAAAGCAG+AGG | + | Chr1:71479145-71479164 | None:intergenic | 50.0% | |
| GAAACAATCACCACAGCCGT+CGG | + | Chr1:71478701-71478720 | None:intergenic | 50.0% | |
| GAAACTGCGACATTTCGTCC+TGG | + | Chr1:71478853-71478872 | None:intergenic | 50.0% | |
| GATCGACGATGACGTTGAGT+GGG | + | Chr1:71478579-71478598 | None:intergenic | 50.0% | |
| GTGGGTGATACTATTGTCCG+GGG | + | Chr1:71478767-71478786 | None:intergenic | 50.0% | |
| TCCTCTAATGGCTTCAACCC+AGG | - | Chr1:71478832-71478851 | MsG0180003992.01.T01:CDS | 50.0% | |
| TGCAAGTGGTGCTTCTGGTA+TGG | - | Chr1:71478918-71478937 | MsG0180003992.01.T01:CDS | 50.0% | |
| ! | ACTGGGAGAACTAGGGCTTT+TGG | + | Chr1:71478493-71478512 | None:intergenic | 50.0% |
| ! | GGTTTTCGCAATCAACCCAG+AGG | + | Chr1:71478615-71478634 | None:intergenic | 50.0% |
| ! | GTAGCGCCATCTTTTGTGAC+TGG | + | Chr1:71478511-71478530 | None:intergenic | 50.0% |
| ! | GTGGGACTAGTGGAGTTCTT+TGG | + | Chr1:71478561-71478580 | None:intergenic | 50.0% |
| ! | TCCTGGGTTGAAGCCATTAG+AGG | + | Chr1:71478836-71478855 | None:intergenic | 50.0% |
| ! | TTGTTGTTGCAGCCAGGCTT+TGG | + | Chr1:71478730-71478749 | None:intergenic | 50.0% |
| !! | GATTGGAGGGTATCAGTTGG+AGG | - | Chr1:71479581-71479600 | MsG0180003992.01.T01:CDS | 50.0% |
| AACGTCATCGTCGATCTCGG+TGG | - | Chr1:71478583-71478602 | MsG0180003992.01.T01:CDS | 55.0% | |
| AGTGGTGCTTCTGGTATGGC+TGG | - | Chr1:71478922-71478941 | MsG0180003992.01.T01:CDS | 55.0% | |
| ATCTCGGTGGCCAATTCCTC+TGG | - | Chr1:71478596-71478615 | MsG0180003992.01.T01:CDS | 55.0% | |
| ATCTTCAGCAAGTTCCCCGC+TGG | + | Chr1:71478797-71478816 | None:intergenic | 55.0% | |
| GGACTTGCAAGTGGTGCTTC+TGG | - | Chr1:71478913-71478932 | MsG0180003992.01.T01:CDS | 55.0% | |
| GGCTGCAACAACAACACCTG+TGG | - | Chr1:71478736-71478755 | MsG0180003992.01.T01:CDS | 55.0% | |
| TCTCGGTGGCCAATTCCTCT+GGG | - | Chr1:71478597-71478616 | MsG0180003992.01.T01:CDS | 55.0% | |
| !! | CAGGTGTTGTTGTTGCAGCC+AGG | + | Chr1:71478736-71478755 | None:intergenic | 55.0% |
| !! | GCTTCTGGTATGGCTGGTCT+TGG | - | Chr1:71478928-71478947 | MsG0180003992.01.T01:CDS | 55.0% |
| ATCACCACAGCCGTCGGAGT+TGG | + | Chr1:71478695-71478714 | None:intergenic | 60.0% | |
| ATTGTCCGGGGTGACACCAC+AGG | + | Chr1:71478755-71478774 | None:intergenic | 60.0% | |
| TTCAGCAAGTTCCCCGCTGG+TGG | + | Chr1:71478794-71478813 | None:intergenic | 60.0% | |
| !! | CAACACCTGTGGTGTCACCC+CGG | - | Chr1:71478747-71478766 | MsG0180003992.01.T01:CDS | 60.0% |
| !! | GTTGGCGAGGGAGCATTGAG+CGG | + | Chr1:71478677-71478696 | None:intergenic | 60.0% |
| ACAGCCGTCGGAGTTGGCGA+GGG | + | Chr1:71478689-71478708 | None:intergenic | 65.0% | |
| AGCAAGTTCCCCGCTGGTGG+CGG | + | Chr1:71478791-71478810 | None:intergenic | 65.0% | |
| ATCACCCACACCGCCACCAG+CGG | - | Chr1:71478778-71478797 | MsG0180003992.01.T01:CDS | 65.0% | |
| TGCTCCCTCGCCAACTCCGA+CGG | - | Chr1:71478682-71478701 | MsG0180003992.01.T01:CDS | 65.0% | |
| TTGAGCGGAGCGACACCGTG+CGG | + | Chr1:71478662-71478681 | None:intergenic | 65.0% | |
| ACACCGTGCGGGGCGGTATG+TGG | + | Chr1:71478650-71478669 | None:intergenic | 70.0% | |
| CACAGCCGTCGGAGTTGGCG+AGG | + | Chr1:71478690-71478709 | None:intergenic | 70.0% | |
| CCTCCACATACCGCCCCGCA+CGG | - | Chr1:71478644-71478663 | MsG0180003992.01.T01:CDS | 70.0% | |
| CTCGCCAACTCCGACGGCTG+TGG | - | Chr1:71478688-71478707 | MsG0180003992.01.T01:CDS | 70.0% | |
| TCACCCACACCGCCACCAGC+GGG | - | Chr1:71478779-71478798 | MsG0180003992.01.T01:CDS | 70.0% | |
| TGAGCGGAGCGACACCGTGC+GGG | + | Chr1:71478661-71478680 | None:intergenic | 70.0% | |
| !! | TTCCCCGCTGGTGGCGGTGT+GGG | + | Chr1:71478785-71478804 | None:intergenic | 70.0% |
| CACCCACACCGCCACCAGCG+GGG | - | Chr1:71478780-71478799 | MsG0180003992.01.T01:CDS | 75.0% | |
| CCGTGCGGGGCGGTATGTGG+AGG | + | Chr1:71478647-71478666 | None:intergenic | 75.0% | |
| GAGCGGAGCGACACCGTGCG+GGG | + | Chr1:71478660-71478679 | None:intergenic | 75.0% | |
| !! | GTTCCCCGCTGGTGGCGGTG+TGG | + | Chr1:71478786-71478805 | None:intergenic | 75.0% |
| ! | CGGAGCGACACCGTGCGGGG+CGG | + | Chr1:71478657-71478676 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 71478406 | 71479710 | 71478406 | ID=MsG0180003992.01;Name=MsG0180003992.01 |
| Chr1 | mRNA | 71478406 | 71479710 | 71478406 | ID=MsG0180003992.01.T01;Parent=MsG0180003992.01;Name=MsG0180003992.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|434 |
| Chr1 | exon | 71478406 | 71479710 | 71478406 | ID=MsG0180003992.01.T01:exon:4855;Parent=MsG0180003992.01.T01 |
| Chr1 | CDS | 71478406 | 71479710 | 71478406 | ID=MsG0180003992.01.T01:cds;Parent=MsG0180003992.01.T01 |
| Gene Sequence |
| Protein sequence |
