AlfalfaGEDB | MsG0580025574.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025574.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025574.01.T01	AES95895.2	80.435	276	39	3	19	284	136	406	1.33E-143	422

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025574.01.T01	G7KF60	80.435	276	39	3	19	284	136	406	6.36e-144	422

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380012471.01	MsG0580025574.01	0.801529	8.625904e-49	4.128905e-46
MsG0580025574.01	MsG0680035692.01	0.804880	1.746516e-49	9.109395e-47
MsG0580025574.01	MsG0880043491.01	0.804768	1.843260e-49	9.585660e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025574.01.T01	MTR_5g030750	80.435	276	39	3	19	284	136	406	1.61e-147	422
MsG0580025574.01.T01	MTR_7g076850	82.609	276	38	2	19	284	140	415	2.63e-146	419

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025574.01.T01	AT3G53440	36.680	259	127	6	34	281	256	488	2.20e-31	123
MsG0580025574.01.T01	AT3G53440	36.680	259	127	6	34	281	256	488	2.20e-31	123

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Find 74 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGAAGAAAAGTAGCG+GGG	0.557669	5:+20729111	MsG0580025574.01.T01:CDS
AAAAGGAAGCACGATGCTGG+TGG	0.652162	5:+20729234	MsG0580025574.01.T01:CDS
AAAAGGAGAAGAAAGCACGG+TGG	0.843786	5:+20729157	MsG0580025574.01.T01:CDS
AAAATCTGTTGCAGGTACCT+GGG	0.591061	5:+20732127	MsG0580025574.01.T01:intron
AAAGAGAAGAAAAGTAGCGG+GGG	0.585434	5:+20729112	MsG0580025574.01.T01:CDS
AAAGTTGATCAAAATCATAA+AGG	0.382393	5:+20732381	MsG0580025574.01.T01:CDS
AAATATACAAAGATCACAGT+AGG	0.592908	5:+20733189	MsG0580025574.01.T01:three_prime_UTR
AAGAAAGAAGAGATTGAAGG+TGG	0.529397	5:+20729202	MsG0580025574.01.T01:CDS
AAGATGCTGCTCTTCTCCTA+AGG	0.500545	5:-20732677	None:intergenic
AAGCAACAAAAGGAAAATCA+AGG	0.462563	5:+20732483	MsG0580025574.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCACCTAAATCTTATGAA+AGG	-	Chr5:20731091-20731110	None:intergenic	30.0%
AACCTTTCATAAGATTTAGG+TGG	+	Chr5:20731086-20731105	MsG0580025574.01.T01:intron	30.0%
AAGCAACAAAAGGAAAATCA+AGG	+	Chr5:20732483-20732502	MsG0580025574.01.T01:CDS	30.0%
AAGTCTCTTTCTAGATTTAG+TGG	+	Chr5:20732555-20732574	MsG0580025574.01.T01:CDS	30.0%
AATGCCTTCTATAAAACCAT+TGG	-	Chr5:20730193-20730212	None:intergenic	30.0%
AATGCTACATCTACAAAACT+TGG	-	Chr5:20733011-20733030	None:intergenic	30.0%
AATTAAGATTTAGGGACCTT+AGG	+	Chr5:20732661-20732680	MsG0580025574.01.T01:CDS	30.0%
ACTAAATACTGTCTACTACA+TGG	-	Chr5:20729648-20729667	None:intergenic	30.0%
AGAGAGAAACTGAAGTTATT+AGG	-	Chr5:20730888-20730907	None:intergenic	30.0%
AGCAACAAAAGGAAAATCAA+GGG	+	Chr5:20732484-20732503	MsG0580025574.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	20729088	20733243	20729088	ID=MsG0580025574.01;Name=MsG0580025574.01
Chr5	mRNA	20729088	20733243	20729088	ID=MsG0580025574.01.T01;Parent=MsG0580025574.01;Name=MsG0580025574.01.T01;_AED=0.13;_eAED=0.14;_QI=0\|0\|0.5\|1\|1\|1\|2\|326\|314
Chr5	exon	20729088	20729255	20729088	ID=MsG0580025574.01.T01:exon:23339;Parent=MsG0580025574.01.T01
Chr5	exon	20732141	20733243	20732141	ID=MsG0580025574.01.T01:exon:23340;Parent=MsG0580025574.01.T01
Chr5	CDS	20729088	20729255	20729088	ID=MsG0580025574.01.T01:cds;Parent=MsG0580025574.01.T01
Chr5	CDS	20732141	20732917	20732141	ID=MsG0580025574.01.T01:cds;Parent=MsG0580025574.01.T01
Chr5	three_prime_UTR	20732918	20733243	20732918	ID=MsG0580025574.01.T01:three_prime_utr;Parent=MsG0580025574.01.T01

Gene Sequence

>MsG0580025574.01.T01

ATGAGAAAAACAGGTCGTGAAGGAAAAGAGAAGAAAAGTAGCGGGGGAGTTGTGGTTGAAGGTGGTAGAAAAAGGAGAAGAAAGCACGGTGGTGATGGAATTGAGGAAGGGAGGAAGAAAGAAGAGATTGAAGGTGGAGTAGAAGGAAAAGGAAGCACGATGCTGGTGGTACCTGGGAAGTCTAAACAAGATTGCTTCGATAGAGTTCACCACGACTTCCACACACCACCTCAATGTCAGCCTCGATCAAGGGCAAAGGCAATTAACTCATCACCCCTTCATCAATTCTCAATATCTCCAAGTAAACTGCTCATACCCACTGGAAAGAAGGTTGGCAAATCAAATATTCTAAAACCAAAGAGCATTGTTACTCAGAAGTCTATTGAGAATCTATTACAGTGCCATCTTAAAGTTGATCAAAATCATAAAGGGGACATATTTTCTGTTCTTGAACCCAACTTTGATTTTTCTACTAATGCTATTCTGCCTAGTCAAGAACTTTGTACTCCAAAGCAACAAAAGGAAAATCAAGGGTTCCTACAAATTTGCAATGAAAGATCATCATCATCTTCAATTCATAAGAAGTCTCTTTCTAGATTTAGTGGCTCATCAGGTGTTAAAGATCTTGCTAGTCCACCTGTGCTAAAGCAAGTAAAAAACAAGGTGCAGCATGAAAAATATGTCAATCAATTAAGATTTAGGGACCTTAGGAGAAGAGCAGCATCTTCAAGGGCAAAAAAATCTGTAGCTGGAGAAGGAAAAGGCATTCAAAAAACAGATGTTGTTAAAGCAGCAAAAGTTGCATTGGTTTCTGAAGCTAGAGATGCTATCAATAAATTTCAGCAATCCAAATCAATTTCATGGGCGACACTTGTAGTTCTGATGAGGACAATGACGATGACACTGGAGCTGAAGATGAAACTCCATAGGCTTTTCTCGAGCTAA

Protein sequence

>MsG0580025574.01.T01

MRKTGREGKEKKSSGGVVVEGGRKRRRKHGGDGIEEGRKKEEIEGGVEGKGSTMLVVPGKSKQDCFDRVHHDFHTPPQCQPRSRAKAINSSPLHQFSISPSKLLIPTGKKVGKSNILKPKSIVTQKSIENLLQCHLKVDQNHKGDIFSVLEPNFDFSTNAILPSQELCTPKQQKENQGFLQICNERSSSSSIHKKSLSRFSGSSGVKDLASPPVLKQVKNKVQHEKYVNQLRFRDLRRRAASSRAKKSVAGEGKGIQKTDVVKAAKVALVSEARDAINKFQQSKSISWATLVVLMRTMTMTLELKMKLHRLFSS*