AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025574.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025574.01.T01 MTR_5g030750 80.435 276 39 3 19 284 136 406 1.61e-147 422
MsG0580025574.01.T01 MTR_7g076850 82.609 276 38 2 19 284 140 415 2.63e-146 419
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025574.01.T01 AT3G53440 36.680 259 127 6 34 281 256 488 2.20e-31 123
MsG0580025574.01.T01 AT3G53440 36.680 259 127 6 34 281 256 488 2.20e-31 123

Find 74 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCAATCAATTAAGATTT+AGG 0.116714 5:+20732652 MsG0580025574.01.T01:CDS
TCCAAACTTCTATTGTTATA+TGG 0.221830 5:+20733076 MsG0580025574.01.T01:three_prime_UTR
TATTTACTCACTGCTAGTTT+TGG 0.292991 5:+20733215 MsG0580025574.01.T01:three_prime_UTR
TGTCAATCAATTAAGATTTA+GGG 0.297311 5:+20732653 MsG0580025574.01.T01:CDS
GAGATTGAAGGTGGAGTAGA+AGG 0.318046 5:+20729211 MsG0580025574.01.T01:CDS
TTCTGAGTAACAATGCTCTT+TGG 0.320344 5:-20732327 None:intergenic
TGTCGCCCATGAAATTGATT+TGG 0.325165 5:-20732820 None:intergenic
AAGTCTCTTTCTAGATTTAG+TGG 0.343248 5:+20732555 MsG0580025574.01.T01:CDS
ATCTTTCATTGCAAATTTGT+AGG 0.343532 5:-20732509 None:intergenic
AGCAATCCAAATCAATTTCA+TGG 0.349553 5:+20732814 MsG0580025574.01.T01:CDS
GCCATATAACAATAGAAGTT+TGG 0.354987 5:-20733077 None:intergenic
AAAGTTGATCAAAATCATAA+AGG 0.382393 5:+20732381 MsG0580025574.01.T01:CDS
CAAAATCTGTTGCAGGTACC+TGG 0.382661 5:+20732126 MsG0580025574.01.T01:intron
TCTAGATTTAGTGGCTCATC+AGG 0.392797 5:+20732564 MsG0580025574.01.T01:CDS
AAGTTGATCAAAATCATAAA+GGG 0.398649 5:+20732382 MsG0580025574.01.T01:CDS
TTCAGATATACATTTAGGTT+GGG 0.405073 5:+20733111 MsG0580025574.01.T01:three_prime_UTR
AATTAAGATTTAGGGACCTT+AGG 0.417162 5:+20732661 MsG0580025574.01.T01:CDS
TGGTTGAAGGTGGTAGAAAA+AGG 0.424066 5:+20729140 MsG0580025574.01.T01:CDS
GAAAAGAGAAGAAAAGTAGC+GGG 0.430036 5:+20729110 MsG0580025574.01.T01:CDS
TTTCAGATATACATTTAGGT+TGG 0.436895 5:+20733110 MsG0580025574.01.T01:three_prime_UTR
GTGGGTATGAGCAGTTTACT+TGG 0.440203 5:-20732270 None:intergenic
GGAGAAGAGCAGCATCTTCA+AGG 0.442712 5:+20732682 MsG0580025574.01.T01:CDS
AATGCTACATCTACAAAACT+TGG 0.459493 5:-20733008 None:intergenic
AAGCAACAAAAGGAAAATCA+AGG 0.462563 5:+20732483 MsG0580025574.01.T01:CDS
GAAGGTGGAGTAGAAGGAAA+AGG 0.479064 5:+20729217 MsG0580025574.01.T01:CDS
CTCAATGTCAGCCTCGATCA+AGG 0.480704 5:+20732202 MsG0580025574.01.T01:CDS
GAGATATTGAGAATTGATGA+AGG 0.499568 5:-20732248 None:intergenic
AAGATGCTGCTCTTCTCCTA+AGG 0.500545 5:-20732677 None:intergenic
TAAAGCAGCAAAAGTTGCAT+TGG 0.501844 5:+20732758 MsG0580025574.01.T01:CDS
AGAAGAAAGCACGGTGGTGA+TGG 0.502654 5:+20729163 MsG0580025574.01.T01:CDS
TTGTACTCCAAAGCAACAAA+AGG 0.506093 5:+20732473 MsG0580025574.01.T01:CDS
TTGCCAACCTTCTTTCCAGT+GGG 0.512778 5:-20732288 None:intergenic
GCTCATACCCACTGGAAAGA+AGG 0.513723 5:+20732281 MsG0580025574.01.T01:CDS
CAATCTTGTTTAGACTTCCC+AGG 0.515115 5:-20732144 None:intergenic
TTTACTTGCTTTAGCACAGG+TGG 0.520137 5:-20732606 None:intergenic
GCAATCCAAATCAATTTCAT+GGG 0.527772 5:+20732815 MsG0580025574.01.T01:CDS
AAGAAAGAAGAGATTGAAGG+TGG 0.529397 5:+20729202 MsG0580025574.01.T01:CDS
AGGAAAAGGAAGCACGATGC+TGG 0.533573 5:+20729231 MsG0580025574.01.T01:CDS
TTTGCCAACCTTCTTTCCAG+TGG 0.543425 5:-20732289 None:intergenic
GGAAAAGAGAAGAAAAGTAG+CGG 0.543653 5:+20729109 MsG0580025574.01.T01:CDS
AGGAAGAAAGAAGAGATTGA+AGG 0.543684 5:+20729199 MsG0580025574.01.T01:CDS
TAATTGTCTTGTATCCGTCT+AGG 0.544557 5:+20733155 MsG0580025574.01.T01:three_prime_UTR
TCTGTAGCTGGAGAAGGAAA+AGG 0.546804 5:+20732714 MsG0580025574.01.T01:CDS
AAAAGAGAAGAAAAGTAGCG+GGG 0.557669 5:+20729111 MsG0580025574.01.T01:CDS
GCACGGTGGTGATGGAATTG+AGG 0.558489 5:+20729171 MsG0580025574.01.T01:CDS
GTGATGGAATTGAGGAAGGG+AGG 0.575565 5:+20729179 MsG0580025574.01.T01:CDS
ATTGCCTTTGCCCTTGATCG+AGG 0.578198 5:-20732213 None:intergenic
ATGCTACATCTACAAAACTT+GGG 0.581912 5:-20733007 None:intergenic
GGTGGTGATGGAATTGAGGA+AGG 0.582281 5:+20729175 MsG0580025574.01.T01:CDS
ATACCCACTGGAAAGAAGGT+TGG 0.583152 5:+20732285 MsG0580025574.01.T01:CDS
GGAGTACAAAGTTCTTGACT+AGG 0.585184 5:-20732459 None:intergenic
AAAGAGAAGAAAAGTAGCGG+GGG 0.585434 5:+20729112 MsG0580025574.01.T01:CDS
AAAATCTGTTGCAGGTACCT+GGG 0.591061 5:+20732127 MsG0580025574.01.T01:intron
AGTAAACTGCTCATACCCAC+TGG 0.591066 5:+20732273 MsG0580025574.01.T01:CDS
AAATATACAAAGATCACAGT+AGG 0.592908 5:+20733189 MsG0580025574.01.T01:three_prime_UTR
ATTAGCTCGAGAAAAGCCTA+TGG 0.593019 5:-20732896 None:intergenic
GAGAAGAGCAGCATCTTCAA+GGG 0.593581 5:+20732683 MsG0580025574.01.T01:CDS
AGATATTGAGAATTGATGAA+GGG 0.597555 5:-20732247 None:intergenic
TCAATGTCAGCCTCGATCAA+GGG 0.602031 5:+20732203 MsG0580025574.01.T01:CDS
AGCAACAAAAGGAAAATCAA+GGG 0.616741 5:+20732484 MsG0580025574.01.T01:CDS
CTTGATCGAGGCTGACATTG+AGG 0.617140 5:-20732201 None:intergenic
TCAGCCTCGATCAAGGGCAA+AGG 0.617327 5:+20732209 MsG0580025574.01.T01:CDS
GATATTGAGAATTGATGAAG+GGG 0.627652 5:-20732246 None:intergenic
GAGGACAATGACGATGACAC+TGG 0.629852 5:+20732857 MsG0580025574.01.T01:CDS
TTTCAAGCTAAGTACCTAGA+CGG 0.630475 5:-20733169 None:intergenic
CGACACTTGTAGTTCTGATG+AGG 0.643806 5:+20732838 MsG0580025574.01.T01:CDS
AGCTGAAGATGAAACTCCAT+AGG 0.645407 5:+20732880 MsG0580025574.01.T01:CDS
AGTTGATCAAAATCATAAAG+GGG 0.648404 5:+20732383 MsG0580025574.01.T01:CDS
AAAAGGAAGCACGATGCTGG+TGG 0.652162 5:+20729234 MsG0580025574.01.T01:CDS
GCTGACATTGAGGTGGTGTG+TGG 0.659104 5:-20732191 None:intergenic
GAGGTGGTGTGTGGAAGTCG+TGG 0.661838 5:-20732182 None:intergenic
GTGGTGATGGAATTGAGGAA+GGG 0.666606 5:+20729176 MsG0580025574.01.T01:CDS
GATCGAGGCTGACATTGAGG+TGG 0.699614 5:-20732198 None:intergenic
AAAAGGAGAAGAAAGCACGG+TGG 0.843786 5:+20729157 MsG0580025574.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTATAATTTCTTGA+TGG + Chr5:20732042-20732061 MsG0580025574.01.T01:intron 10.0%
!!! AATTTTTCAGATATACATTT+AGG + Chr5:20733106-20733125 MsG0580025574.01.T01:three_prime_UTR 15.0%
!!! TTTGAATTTTGATATTATGT+TGG + Chr5:20730998-20731017 MsG0580025574.01.T01:intron 15.0%
!! AATTATAGTGTTTAATCCAA+TGG + Chr5:20730174-20730193 MsG0580025574.01.T01:intron 20.0%
!! ATGTCAATCAATTAAGATTT+AGG + Chr5:20732652-20732671 MsG0580025574.01.T01:CDS 20.0%
!! ATTAGTAGAAAAATCAAAGT+TGG - Chr5:20732430-20732449 None:intergenic 20.0%
!! TAAAACCTTTCATAAGATTT+AGG + Chr5:20731083-20731102 MsG0580025574.01.T01:intron 20.0%
!! TGATATGTGACATAATATTA+TGG + Chr5:20729611-20729630 MsG0580025574.01.T01:intron 20.0%
!! TGTCAATCAATTAAGATTTA+GGG + Chr5:20732653-20732672 MsG0580025574.01.T01:CDS 20.0%
!! TTAGTAGAAAAATCAAAGTT+GGG - Chr5:20732429-20732448 None:intergenic 20.0%
!! TTATATTTACAGTTACCTTT+AGG - Chr5:20731768-20731787 None:intergenic 20.0%
!! TTTAAAGAACAACAAAACAA+AGG - Chr5:20731466-20731485 None:intergenic 20.0%
!!! AAAGTTGATCAAAATCATAA+AGG + Chr5:20732381-20732400 MsG0580025574.01.T01:CDS 20.0%
!!! AAGTTGATCAAAATCATAAA+GGG + Chr5:20732382-20732401 MsG0580025574.01.T01:CDS 20.0%
!!! AATTATCAGTTCTTTTCTTT+AGG + Chr5:20730946-20730965 MsG0580025574.01.T01:intron 20.0%
!!! ACAACTACAAATATTTTTCA+TGG - Chr5:20730272-20730291 None:intergenic 20.0%
!!! CTTGGTTTTAAAAAAAACAA+GGG + Chr5:20730840-20730859 MsG0580025574.01.T01:intron 20.0%
!!! GGATTTAATGTTTTGAATTT+TGG + Chr5:20730967-20730986 MsG0580025574.01.T01:intron 20.0%
!!! TATTACATTTTTCTTTGTGA+TGG - Chr5:20732025-20732044 None:intergenic 20.0%
!!! TTAATCCAATAGTTTTATAG+AGG + Chr5:20731643-20731662 MsG0580025574.01.T01:intron 20.0%
!!! TTTAAAGTTTTAGACATTGT+AGG + Chr5:20732957-20732976 MsG0580025574.01.T01:three_prime_UTR 20.0%
!!! TTTCGTAATGTTTTGAATTT+TGG + Chr5:20731033-20731052 MsG0580025574.01.T01:intron 20.0%
!!! TTTTCTCATATAGTTTTTCT+AGG - Chr5:20730381-20730400 None:intergenic 20.0%
! AAACTGTGAAGAAGATTAAT+GGG + Chr5:20729575-20729594 MsG0580025574.01.T01:intron 25.0%
! AAATATACAAAGATCACAGT+AGG + Chr5:20733189-20733208 MsG0580025574.01.T01:three_prime_UTR 25.0%
! AGACAGTATTTAGTGTTATA+AGG + Chr5:20729654-20729673 MsG0580025574.01.T01:intron 25.0%
! AGATATTGAGAATTGATGAA+GGG - Chr5:20732250-20732269 None:intergenic 25.0%
! AGTTGATCAAAATCATAAAG+GGG + Chr5:20732383-20732402 MsG0580025574.01.T01:CDS 25.0%
! ATCTTTCATTGCAAATTTGT+AGG - Chr5:20732512-20732531 None:intergenic 25.0%
! ATTACTTGTCCAAATTAATG+AGG + Chr5:20730351-20730370 MsG0580025574.01.T01:intron 25.0%
! ATTTCATAAAGATGTCTGAA+TGG - Chr5:20731950-20731969 None:intergenic 25.0%
! CTTTCATGTTTATCGAATAT+CGG + Chr5:20731798-20731817 MsG0580025574.01.T01:intron 25.0%
! GAAAAACAACAACAAAACAA+AGG - Chr5:20730005-20730024 None:intergenic 25.0%
! GTATTTAGTGTTATAAGGTT+TGG + Chr5:20729659-20729678 MsG0580025574.01.T01:intron 25.0%
! TAATATATACAGATCGCTTA+TGG + Chr5:20730442-20730461 MsG0580025574.01.T01:intron 25.0%
! TATCAAACTCATTAATCACT+AGG - Chr5:20730416-20730435 None:intergenic 25.0%
! TCCAAACTTCTATTGTTATA+TGG + Chr5:20733076-20733095 MsG0580025574.01.T01:three_prime_UTR 25.0%
! TCCTAAAAGTTATGAAATAG+CGG + Chr5:20731217-20731236 MsG0580025574.01.T01:intron 25.0%
! TCTATGTGTTTATTTCCTAA+AGG + Chr5:20731750-20731769 MsG0580025574.01.T01:intron 25.0%
! TCTTCTTCTTTCATTCTTTA+CGG + Chr5:20730905-20730924 MsG0580025574.01.T01:intron 25.0%
! TTGAATTATGTGTTTAAACG+AGG + Chr5:20729529-20729548 MsG0580025574.01.T01:intron 25.0%
! TTTCACGTAAAATGAAATAG+CGG - Chr5:20730807-20730826 None:intergenic 25.0%
! TTTCAGATATACATTTAGGT+TGG + Chr5:20733110-20733129 MsG0580025574.01.T01:three_prime_UTR 25.0%
!! AGTGATTAATTTTGAGCAAA+TGG - Chr5:20729942-20729961 None:intergenic 25.0%
!! TTCAGATATACATTTAGGTT+GGG + Chr5:20733111-20733130 MsG0580025574.01.T01:three_prime_UTR 25.0%
!! TTCAGTAAGCTAAATTTGTT+TGG + Chr5:20729713-20729732 MsG0580025574.01.T01:intron 25.0%
!!! ACAGAATCACTTTTACTTTT+CGG + Chr5:20731971-20731990 MsG0580025574.01.T01:intron 25.0%
!!! CCTTGGTTTTAAAAAAAACA+AGG + Chr5:20730839-20730858 MsG0580025574.01.T01:intron 25.0%
!!! CCTTGTTTTTTTTAAAACCA+AGG - Chr5:20730842-20730861 None:intergenic 25.0%
!!! GATTTTGATCAACTTTAAGA+TGG - Chr5:20732377-20732396 None:intergenic 25.0%
!!! TAATCCAATGGTTTTATAGA+AGG + Chr5:20730186-20730205 MsG0580025574.01.T01:intron 25.0%
AACCACCTAAATCTTATGAA+AGG - Chr5:20731091-20731110 None:intergenic 30.0%
AACCTTTCATAAGATTTAGG+TGG + Chr5:20731086-20731105 MsG0580025574.01.T01:intron 30.0%
AAGCAACAAAAGGAAAATCA+AGG + Chr5:20732483-20732502 MsG0580025574.01.T01:CDS 30.0%
AAGTCTCTTTCTAGATTTAG+TGG + Chr5:20732555-20732574 MsG0580025574.01.T01:CDS 30.0%
AATGCCTTCTATAAAACCAT+TGG - Chr5:20730193-20730212 None:intergenic 30.0%
AATGCTACATCTACAAAACT+TGG - Chr5:20733011-20733030 None:intergenic 30.0%
AATTAAGATTTAGGGACCTT+AGG + Chr5:20732661-20732680 MsG0580025574.01.T01:CDS 30.0%
ACTAAATACTGTCTACTACA+TGG - Chr5:20729648-20729667 None:intergenic 30.0%
AGAGAGAAACTGAAGTTATT+AGG - Chr5:20730888-20730907 None:intergenic 30.0%
AGCAACAAAAGGAAAATCAA+GGG + Chr5:20732484-20732503 MsG0580025574.01.T01:CDS 30.0%
AGCAATCCAAATCAATTTCA+TGG + Chr5:20732814-20732833 MsG0580025574.01.T01:CDS 30.0%
ATGCTACATCTACAAAACTT+GGG - Chr5:20733010-20733029 None:intergenic 30.0%
CAACACTATTAGTAAATAGC+TGG + Chr5:20729776-20729795 MsG0580025574.01.T01:intron 30.0%
CTAACCATAGTAGTTAATAG+CGG - Chr5:20731303-20731322 None:intergenic 30.0%
GAAAAGAACTGATAATTCCA+AGG - Chr5:20730943-20730962 None:intergenic 30.0%
GAAACTGTGAAGAAGATTAA+TGG + Chr5:20729574-20729593 MsG0580025574.01.T01:intron 30.0%
GAAGTTTCAAATCTCACAAT+TGG - Chr5:20731371-20731390 None:intergenic 30.0%
GAGAGAAACTGAAGTTATTA+GGG - Chr5:20730887-20730906 None:intergenic 30.0%
GAGATATTGAGAATTGATGA+AGG - Chr5:20732251-20732270 None:intergenic 30.0%
GATATTGAGAATTGATGAAG+GGG - Chr5:20732249-20732268 None:intergenic 30.0%
GCAATCCAAATCAATTTCAT+GGG + Chr5:20732815-20732834 MsG0580025574.01.T01:CDS 30.0%
GCCATATAACAATAGAAGTT+TGG - Chr5:20733080-20733099 None:intergenic 30.0%
GCTAAAGCAAGTAAAAAACA+AGG + Chr5:20732614-20732633 MsG0580025574.01.T01:CDS 30.0%
GTGAGCATTTGAATTATTCT+GGG + Chr5:20731882-20731901 MsG0580025574.01.T01:intron 30.0%
TAAGCACATATGCACTTATA+TGG - Chr5:20732079-20732098 None:intergenic 30.0%
TCTTACATACAGAGAAAGAT+AGG - Chr5:20729441-20729460 None:intergenic 30.0%
TGAAAATACAAGATTCTCCT+TGG + Chr5:20730822-20730841 MsG0580025574.01.T01:intron 30.0%
TGAGCATTTGAATTATTCTG+GGG + Chr5:20731883-20731902 MsG0580025574.01.T01:intron 30.0%
TGTTTATCGAATATCGGATT+TGG + Chr5:20731804-20731823 MsG0580025574.01.T01:intron 30.0%
TTTCAATAACAACACCATGT+TGG - Chr5:20731727-20731746 None:intergenic 30.0%
! ATTGTGAGATTTGAAACTTC+TGG + Chr5:20731371-20731390 MsG0580025574.01.T01:intron 30.0%
! CAAAACAAAGGCATAAGATA+AGG - Chr5:20729993-20730012 None:intergenic 30.0%
! CTACTAACTAGTTTAAACCT+AGG - Chr5:20729811-20729830 None:intergenic 30.0%
! CTCAGTAAGCTAAATTTGTT+CGG + Chr5:20730540-20730559 MsG0580025574.01.T01:intron 30.0%
! TTGTGAGATTTGAAACTTCT+GGG + Chr5:20731372-20731391 MsG0580025574.01.T01:intron 30.0%
!! AAGTTTTATCCCACTAAGTA+GGG - Chr5:20730491-20730510 None:intergenic 30.0%
!! ACCGCTATTTCATAACTTTT+AGG - Chr5:20731221-20731240 None:intergenic 30.0%
!! CGTAATGTTTTGAATTTTGG+TGG + Chr5:20731036-20731055 MsG0580025574.01.T01:intron 30.0%
!! TATTTACTCACTGCTAGTTT+TGG + Chr5:20733215-20733234 MsG0580025574.01.T01:three_prime_UTR 30.0%
!! TGATTTTCCTTTTGTTGCTT+TGG - Chr5:20732483-20732502 None:intergenic 30.0%
!! TTTATAGAAGGCATTGCATA+GGG + Chr5:20730198-20730217 MsG0580025574.01.T01:intron 30.0%
!!! ATGACGATTTTGGTTTATCA+CGG + Chr5:20729967-20729986 MsG0580025574.01.T01:intron 30.0%
!!! TTTTATAGAAGGCATTGCAT+AGG + Chr5:20730197-20730216 MsG0580025574.01.T01:intron 30.0%
!!! TTTTTTACTTGCTTTAGCAC+AGG - Chr5:20732612-20732631 None:intergenic 30.0%
AAAAAATCTGTAGCTGGAGA+AGG + Chr5:20732708-20732727 MsG0580025574.01.T01:CDS 35.0%
AAAAGAGAAGAAAAGTAGCG+GGG + Chr5:20729111-20729130 MsG0580025574.01.T01:CDS 35.0%
AAATTCGTCAAGTATCAGCA+AGG + Chr5:20729751-20729770 MsG0580025574.01.T01:intron 35.0%
AAGAAAGAAGAGATTGAAGG+TGG + Chr5:20729202-20729221 MsG0580025574.01.T01:CDS 35.0%
AAGTTGAATGTGGTGTGAAA+AGG + Chr5:20729286-20729305 MsG0580025574.01.T01:intron 35.0%
AGAAAAAGGAGAAGAAAGCA+CGG + Chr5:20729154-20729173 MsG0580025574.01.T01:CDS 35.0%
AGGAAGAAAGAAGAGATTGA+AGG + Chr5:20729199-20729218 MsG0580025574.01.T01:CDS 35.0%
AGTAGTGTGTATGTCTAGAA+AGG + Chr5:20729826-20729845 MsG0580025574.01.T01:intron 35.0%
CAACACAATTGGTAAATAGC+TGG + Chr5:20730596-20730615 MsG0580025574.01.T01:intron 35.0%
CTACTTAGTGGGATAAAACT+TGG + Chr5:20730490-20730509 MsG0580025574.01.T01:intron 35.0%
CTAGGTCTTCCTCATTAATT+TGG - Chr5:20730363-20730382 None:intergenic 35.0%
CTATCCGCTATTAACTACTA+TGG + Chr5:20731296-20731315 MsG0580025574.01.T01:intron 35.0%
GAAAAGAGAAGAAAAGTAGC+GGG + Chr5:20729110-20729129 MsG0580025574.01.T01:CDS 35.0%
GATAATTCCAAGGAGATGAA+AGG - Chr5:20730933-20730952 None:intergenic 35.0%
GGAAAAGAGAAGAAAAGTAG+CGG + Chr5:20729109-20729128 MsG0580025574.01.T01:CDS 35.0%
GGTGAGCATTTGAATTATTC+TGG + Chr5:20731881-20731900 MsG0580025574.01.T01:intron 35.0%
TAAAGCAGCAAAAGTTGCAT+TGG + Chr5:20732758-20732777 MsG0580025574.01.T01:CDS 35.0%
TAACAACACCATGTTGGAAA+CGG - Chr5:20731721-20731740 None:intergenic 35.0%
TAATTGTCTTGTATCCGTCT+AGG + Chr5:20733155-20733174 MsG0580025574.01.T01:three_prime_UTR 35.0%
TATGGTTAGCGTGTATAGAA+AGG + Chr5:20731314-20731333 MsG0580025574.01.T01:intron 35.0%
TCACTCAAAGACGATAAGTT+TGG + Chr5:20731419-20731438 MsG0580025574.01.T01:intron 35.0%
TCGTCTTTGAGTGAATCTTT+CGG - Chr5:20731413-20731432 None:intergenic 35.0%
TGCAGCCTCTATAAAACTAT+TGG - Chr5:20731651-20731670 None:intergenic 35.0%
TGTACAGCAATTTCAATGAC+CGG - Chr5:20732108-20732127 None:intergenic 35.0%
TTCTGAGTAACAATGCTCTT+TGG - Chr5:20732330-20732349 None:intergenic 35.0%
TTGTACTCCAAAGCAACAAA+AGG + Chr5:20732473-20732492 MsG0580025574.01.T01:CDS 35.0%
TTTCAAGCTAAGTACCTAGA+CGG - Chr5:20733172-20733191 None:intergenic 35.0%
! CGAACAAATTTAGCTTACTG+AGG - Chr5:20730542-20730561 None:intergenic 35.0%
! CTGTTTTCACATGACATTTC+AGG - Chr5:20730051-20730070 None:intergenic 35.0%
! GCTAACTAGTTTGAACCTAT+AGG - Chr5:20730631-20730650 None:intergenic 35.0%
! TCACTCAAAGATGACGATTT+TGG + Chr5:20729957-20729976 MsG0580025574.01.T01:intron 35.0%
! TGTGAAGAAGATTAATGGGT+TGG + Chr5:20729579-20729598 MsG0580025574.01.T01:intron 35.0%
!! CAAGTTTTATCCCACTAAGT+AGG - Chr5:20730492-20730511 None:intergenic 35.0%
!! GATAGAGAAAGCATTGTAGT+GGG + Chr5:20731557-20731576 MsG0580025574.01.T01:intron 35.0%
!! TAGTAGTTAATAGCGGATAG+CGG - Chr5:20731296-20731315 None:intergenic 35.0%
!! TGATAGAGAAAGCATTGTAG+TGG + Chr5:20731556-20731575 MsG0580025574.01.T01:intron 35.0%
AAAATCTGTTGCAGGTACCT+GGG + Chr5:20732127-20732146 MsG0580025574.01.T01:intron 40.0%
AAAGAGAAGAAAAGTAGCGG+GGG + Chr5:20729112-20729131 MsG0580025574.01.T01:CDS 40.0%
ACTAATCTGCGCTGAGATTA+AGG - Chr5:20729883-20729902 None:intergenic 40.0%
AGAGGTAGACACATACATTG+CGG + Chr5:20730226-20730245 MsG0580025574.01.T01:intron 40.0%
AGCGTCAGAGTAGTTAATAG+CGG + Chr5:20730648-20730667 MsG0580025574.01.T01:intron 40.0%
AGCTGAAGATGAAACTCCAT+AGG + Chr5:20732880-20732899 MsG0580025574.01.T01:CDS 40.0%
AGGAAGGAAGAAGTTGAATG+TGG + Chr5:20729276-20729295 MsG0580025574.01.T01:intron 40.0%
AGGGCAAAAAAATCTGTAGC+TGG + Chr5:20732702-20732721 MsG0580025574.01.T01:CDS 40.0%
ATAGCTGGATAATGTGTCCT+AGG + Chr5:20729791-20729810 MsG0580025574.01.T01:intron 40.0%
ATGAGAAAAACAGGTCGTGA+AGG + Chr5:20729088-20729107 MsG0580025574.01.T01:CDS 40.0%
ATGTCTAGAAAGGTCTTCTC+AGG + Chr5:20729836-20729855 MsG0580025574.01.T01:intron 40.0%
ATGTTCCGTATGGTAACTGT+TGG + Chr5:20731673-20731692 MsG0580025574.01.T01:intron 40.0%
ATTAGCTCGAGAAAAGCCTA+TGG - Chr5:20732899-20732918 None:intergenic 40.0%
CAAAACCTAGTCCTCATTTC+TGG + Chr5:20729391-20729410 MsG0580025574.01.T01:intron 40.0%
CATACTATGAAACCCGTCAA+GGG + Chr5:20730563-20730582 MsG0580025574.01.T01:intron 40.0%
CGTCTTTGAGTGAATCTTTC+GGG - Chr5:20731412-20731431 None:intergenic 40.0%
GAAACGTTCTTCCAGAAATG+AGG - Chr5:20729405-20729424 None:intergenic 40.0%
GAGATCCAACAGTTACCATA+CGG - Chr5:20731681-20731700 None:intergenic 40.0%
GATGTTAACCGTTTCCAACA+TGG + Chr5:20731710-20731729 MsG0580025574.01.T01:intron 40.0%
GCAGCAAAGTTCAACACAAT+TGG + Chr5:20730585-20730604 MsG0580025574.01.T01:intron 40.0%
GCATATGTGCTTATTTGCTC+CGG + Chr5:20732086-20732105 MsG0580025574.01.T01:intron 40.0%
GCTGCACACAATGATAAAGA+AGG + Chr5:20730086-20730105 MsG0580025574.01.T01:intron 40.0%
GGAGTACAAAGTTCTTGACT+AGG - Chr5:20732462-20732481 None:intergenic 40.0%
TCAAGCTTGCAATTGCATGA+GGG + Chr5:20731906-20731925 MsG0580025574.01.T01:intron 40.0%
TCTAGATTTAGTGGCTCATC+AGG + Chr5:20732564-20732583 MsG0580025574.01.T01:CDS 40.0%
TGCTGTACAAAATCTGTTGC+AGG + Chr5:20732119-20732138 MsG0580025574.01.T01:intron 40.0%
TGGTTGAAGGTGGTAGAAAA+AGG + Chr5:20729140-20729159 MsG0580025574.01.T01:CDS 40.0%
TGTAGTTGACCCTACTTAGT+GGG + Chr5:20730479-20730498 MsG0580025574.01.T01:intron 40.0%
TGTCGCCCATGAAATTGATT+TGG - Chr5:20732823-20732842 None:intergenic 40.0%
TTCTTCCAGAAATGAGGACT+AGG - Chr5:20729399-20729418 None:intergenic 40.0%
TTGTAGTTGACCCTACTTAG+TGG + Chr5:20730478-20730497 MsG0580025574.01.T01:intron 40.0%
TTTACTTGCTTTAGCACAGG+TGG - Chr5:20732609-20732628 None:intergenic 40.0%
! AATAGTGGAAGAGTTGAGGA+AGG + Chr5:20729260-20729279 MsG0580025574.01.T01:intron 40.0%
! CAATCTTGTTTAGACTTCCC+AGG - Chr5:20732147-20732166 None:intergenic 40.0%
! CTATCCCGCTATAGCATTTT+GGG + Chr5:20731255-20731274 MsG0580025574.01.T01:intron 40.0%
! GTGAAATAGTGGAAGAGTTG+AGG + Chr5:20729256-20729275 MsG0580025574.01.T01:intron 40.0%
! GTGGACTAAGGAACAAGAAT+TGG + Chr5:20729344-20729363 MsG0580025574.01.T01:intron 40.0%
! TATCCCGCTATAGCATTTTG+GGG + Chr5:20731256-20731275 MsG0580025574.01.T01:intron 40.0%
!! ATAAAGAAGGCATTGCAGCA+TGG + Chr5:20730099-20730118 MsG0580025574.01.T01:intron 40.0%
AAAACCGCTATGCAGTGTTG+CGG - Chr5:20730773-20730792 None:intergenic 45.0%
AACTGTTGGATCTCGACGTT+TGG + Chr5:20731687-20731706 MsG0580025574.01.T01:intron 45.0%
AAGATGCTGCTCTTCTCCTA+AGG - Chr5:20732680-20732699 None:intergenic 45.0%
ACTGTTGGATCTCGACGTTT+GGG + Chr5:20731688-20731707 MsG0580025574.01.T01:intron 45.0%
AGTAAACTGCTCATACCCAC+TGG + Chr5:20732273-20732292 MsG0580025574.01.T01:CDS 45.0%
AGTTAATAGCGGCGCTATAG+CGG + Chr5:20730659-20730678 MsG0580025574.01.T01:intron 45.0%
ATACCCACTGGAAAGAAGGT+TGG + Chr5:20732285-20732304 MsG0580025574.01.T01:CDS 45.0%
CAAAATCTGTTGCAGGTACC+TGG + Chr5:20732126-20732145 MsG0580025574.01.T01:intron 45.0%
CAAGCTTGCAATTGCATGAG+GGG + Chr5:20731907-20731926 MsG0580025574.01.T01:intron 45.0%
CATGACCACGTGATCTATCT+AGG + Chr5:20731860-20731879 MsG0580025574.01.T01:intron 45.0%
CTAGATAGATCACGTGGTCA+TGG - Chr5:20731862-20731881 None:intergenic 45.0%
CTGGAAGAACGTTTCGAAAC+TGG + Chr5:20729410-20729429 MsG0580025574.01.T01:intron 45.0%
GAAGGTGGAGTAGAAGGAAA+AGG + Chr5:20729217-20729236 MsG0580025574.01.T01:CDS 45.0%
GAGAAGAGCAGCATCTTCAA+GGG + Chr5:20732683-20732702 MsG0580025574.01.T01:CDS 45.0%
GAGATTGAAGGTGGAGTAGA+AGG + Chr5:20729211-20729230 MsG0580025574.01.T01:CDS 45.0%
GAGTGAATCTTTCGGGCAAA+TGG - Chr5:20731405-20731424 None:intergenic 45.0%
GCATACTATGAAACCCGTCA+AGG + Chr5:20730562-20730581 MsG0580025574.01.T01:intron 45.0%
GTCAAGCTTGCAATTGCATG+AGG + Chr5:20731905-20731924 MsG0580025574.01.T01:intron 45.0%
GTGAAAAGGAAGAGAAAGCG+CGG + Chr5:20729300-20729319 MsG0580025574.01.T01:intron 45.0%
GTGGGTATGAGCAGTTTACT+TGG - Chr5:20732273-20732292 None:intergenic 45.0%
GTGTATAGAAAGGCCATGTC+CGG + Chr5:20731324-20731343 MsG0580025574.01.T01:intron 45.0%
TATCTCATAACGCTACGCCA+TGG - Chr5:20730735-20730754 None:intergenic 45.0%
TCAATGTCAGCCTCGATCAA+GGG + Chr5:20732203-20732222 MsG0580025574.01.T01:CDS 45.0%
TCGACCCCAAAATGCTATAG+CGG - Chr5:20731263-20731282 None:intergenic 45.0%
TCTCAGGAGAGTCAAAACAC+AGG + Chr5:20729852-20729871 MsG0580025574.01.T01:intron 45.0%
TCTGTAGCTGGAGAAGGAAA+AGG + Chr5:20732714-20732733 MsG0580025574.01.T01:CDS 45.0%
TGCGGTCTCTATAGAACTGT+TGG + Chr5:20731605-20731624 MsG0580025574.01.T01:intron 45.0%
TTGAACTTTGCTGCCCTTGA+CGG - Chr5:20730579-20730598 None:intergenic 45.0%
TTGCCAACCTTCTTTCCAGT+GGG - Chr5:20732291-20732310 None:intergenic 45.0%
TTTGCCAACCTTCTTTCCAG+TGG - Chr5:20732292-20732311 None:intergenic 45.0%
! AAAAGGAGAAGAAAGCACGG+TGG + Chr5:20729157-20729176 MsG0580025574.01.T01:CDS 45.0%
! GCTATCCCGCTATAGCATTT+TGG + Chr5:20731254-20731273 MsG0580025574.01.T01:intron 45.0%
!! CGACACTTGTAGTTCTGATG+AGG + Chr5:20732838-20732857 MsG0580025574.01.T01:CDS 45.0%
!! GTGGTGATGGAATTGAGGAA+GGG + Chr5:20729176-20729195 MsG0580025574.01.T01:CDS 45.0%
ACTAATCCGCTCTGACTCTC+CGG - Chr5:20731346-20731365 None:intergenic 50.0%
AGCTGGATCGTGTGTCCTAT+AGG + Chr5:20730613-20730632 MsG0580025574.01.T01:intron 50.0%
AGGCTGCACAATGTTCCGTA+TGG + Chr5:20731663-20731682 MsG0580025574.01.T01:intron 50.0%
ATTGCCTTTGCCCTTGATCG+AGG - Chr5:20732216-20732235 None:intergenic 50.0%
CGACCCCAAAATGCTATAGC+GGG - Chr5:20731262-20731281 None:intergenic 50.0%
CGCCATGGCTTATTGCATAG+CGG - Chr5:20730720-20730739 None:intergenic 50.0%
CTCAATGTCAGCCTCGATCA+AGG + Chr5:20732202-20732221 MsG0580025574.01.T01:CDS 50.0%
CTTGATCGAGGCTGACATTG+AGG - Chr5:20732204-20732223 None:intergenic 50.0%
GAAATAGCGGTCATAGCGCT+AGG + Chr5:20731230-20731249 MsG0580025574.01.T01:intron 50.0%
GACCGCTATGCAATAAGCCA+TGG + Chr5:20730715-20730734 MsG0580025574.01.T01:intron 50.0%
GAGGACAATGACGATGACAC+TGG + Chr5:20732857-20732876 MsG0580025574.01.T01:CDS 50.0%
GCTCACCTAGATAGATCACG+TGG - Chr5:20731868-20731887 None:intergenic 50.0%
GCTCATACCCACTGGAAAGA+AGG + Chr5:20732281-20732300 MsG0580025574.01.T01:CDS 50.0%
GGAGAAGAGCAGCATCTTCA+AGG + Chr5:20732682-20732701 MsG0580025574.01.T01:CDS 50.0%
GGCGTAGCGTTATGAGATAG+CGG + Chr5:20730736-20730755 MsG0580025574.01.T01:intron 50.0%
TGAACTTTGCTGCCCTTGAC+GGG - Chr5:20730578-20730597 None:intergenic 50.0%
TGGCTTATTGCATAGCGGTC+CGG - Chr5:20730715-20730734 None:intergenic 50.0%
! AAAAGGAAGAGAAAGCGCGG+TGG + Chr5:20729303-20729322 MsG0580025574.01.T01:intron 50.0%
! AAAAGGAAGCACGATGCTGG+TGG + Chr5:20729234-20729253 MsG0580025574.01.T01:CDS 50.0%
! AGAAGAAAGCACGGTGGTGA+TGG + Chr5:20729163-20729182 MsG0580025574.01.T01:CDS 50.0%
! AGGAAAAGGAAGCACGATGC+TGG + Chr5:20729231-20729250 MsG0580025574.01.T01:CDS 50.0%
! ATGAGATAGCGGCGCAATAG+CGG + Chr5:20730747-20730766 MsG0580025574.01.T01:intron 50.0%
! CGATGCTGGTGGTGAAATAG+TGG + Chr5:20729245-20729264 MsG0580025574.01.T01:intron 50.0%
! GTGATGGAATTGAGGAAGGG+AGG + Chr5:20729179-20729198 MsG0580025574.01.T01:CDS 50.0%
! GTGGTGGTGAACTTGCACAA+GGG + Chr5:20729322-20729341 MsG0580025574.01.T01:intron 50.0%
!! ACTTGCACAAGGGTGGACTA+AGG + Chr5:20729332-20729351 MsG0580025574.01.T01:intron 50.0%
!! GCATTGTAGTGGGCTTACAG+CGG + Chr5:20731567-20731586 MsG0580025574.01.T01:intron 50.0%
!! GGTGGTGATGGAATTGAGGA+AGG + Chr5:20729175-20729194 MsG0580025574.01.T01:CDS 50.0%
CATTGTGTGCAGCATCGCAG+CGG - Chr5:20730079-20730098 None:intergenic 55.0%
CATTGTGTGCCACATCGCAG+CGG - Chr5:20731538-20731557 None:intergenic 55.0%
GATCGAGGCTGACATTGAGG+TGG - Chr5:20732201-20732220 None:intergenic 55.0%
GCATTGCATAGGGCTTGCAG+AGG + Chr5:20730208-20730227 MsG0580025574.01.T01:intron 55.0%
GCTGACATTGAGGTGGTGTG+TGG - Chr5:20732194-20732213 None:intergenic 55.0%
GTGGTGAACTTGCACAAGGG+TGG + Chr5:20729325-20729344 MsG0580025574.01.T01:intron 55.0%
TACGGCGCCTTTCATCTCCT+TGG + Chr5:20730923-20730942 MsG0580025574.01.T01:intron 55.0%
TCAGCCTCGATCAAGGGCAA+AGG + Chr5:20732209-20732228 MsG0580025574.01.T01:CDS 55.0%
! CATTGCAGCATGGCTTGCAG+TGG + Chr5:20730109-20730128 MsG0580025574.01.T01:intron 55.0%
! GAAAAGTAGCGGGGGAGTTG+TGG + Chr5:20729120-20729139 MsG0580025574.01.T01:CDS 55.0%
! GGTGGTGGTGAACTTGCACA+AGG + Chr5:20729321-20729340 MsG0580025574.01.T01:intron 55.0%
!! GCACGGTGGTGATGGAATTG+AGG + Chr5:20729171-20729190 MsG0580025574.01.T01:CDS 55.0%
AGCGGGGGAGTTGTGGTTGA+AGG + Chr5:20729127-20729146 MsG0580025574.01.T01:CDS 60.0%
CCATGTCCGGAGAGTCAGAG+CGG + Chr5:20731337-20731356 MsG0580025574.01.T01:intron 60.0%
CGGTAGAGCTGCAGACAGTG+CGG + Chr5:20731587-20731606 MsG0580025574.01.T01:intron 60.0%
GAGGTGGTGTGTGGAAGTCG+TGG - Chr5:20732185-20732204 None:intergenic 60.0%
GGCGCTATAGCGGCCAATAG+CGG + Chr5:20730669-20730688 MsG0580025574.01.T01:intron 60.0%
GGGGGAGTTGTGGTTGAAGG+TGG + Chr5:20729130-20729149 MsG0580025574.01.T01:CDS 60.0%
TGGTAGAGTCGCACGCAGTG+TGG + Chr5:20730129-20730148 MsG0580025574.01.T01:intron 60.0%
! AGGAAGAGAAAGCGCGGTGG+TGG + Chr5:20729306-20729325 MsG0580025574.01.T01:intron 60.0%
ACAGTGCAGCCGCTGCGATG+TGG + Chr5:20730067-20730086 MsG0580025574.01.T01:intron 65.0%
CCGCTCTGACTCTCCGGACA+TGG - Chr5:20731340-20731359 None:intergenic 65.0%
GCGGCCGCAACACTGCATAG+CGG + Chr5:20730766-20730785 MsG0580025574.01.T01:intron 65.0%
! TAGCGGATAGCGGCGCGTAG+CGG - Chr5:20731286-20731305 None:intergenic 65.0%
AGCGGCCAATAGCGGCCGCG+CGG + Chr5:20730677-20730696 MsG0580025574.01.T01:intron 75.0%
CCAATAGCGGCCGCGCGGTG+CGG + Chr5:20730682-20730701 MsG0580025574.01.T01:intron 75.0%
! CCGCACCGCGCGGCCGCTAT+TGG - Chr5:20730685-20730704 None:intergenic 80.0%
! CGCGCGGTGCGGTGCGGTAC+CGG + Chr5:20730693-20730712 MsG0580025574.01.T01:intron 80.0%
! CGGTACCGCACCGCACCGCG+CGG - Chr5:20730695-20730714 None:intergenic 80.0%
! AGCGGCCGCGCGGTGCGGTG+CGG + Chr5:20730687-20730706 MsG0580025574.01.T01:intron 85.0%
Chromosome Type Strat End Strand Name
Chr5 gene 20729088 20733243 20729088 ID=MsG0580025574.01;Name=MsG0580025574.01
Chr5 mRNA 20729088 20733243 20729088 ID=MsG0580025574.01.T01;Parent=MsG0580025574.01;Name=MsG0580025574.01.T01;_AED=0.13;_eAED=0.14;_QI=0|0|0.5|1|1|1|2|326|314
Chr5 exon 20729088 20729255 20729088 ID=MsG0580025574.01.T01:exon:23339;Parent=MsG0580025574.01.T01
Chr5 exon 20732141 20733243 20732141 ID=MsG0580025574.01.T01:exon:23340;Parent=MsG0580025574.01.T01
Chr5 CDS 20729088 20729255 20729088 ID=MsG0580025574.01.T01:cds;Parent=MsG0580025574.01.T01
Chr5 CDS 20732141 20732917 20732141 ID=MsG0580025574.01.T01:cds;Parent=MsG0580025574.01.T01
Chr5 three_prime_UTR 20732918 20733243 20732918 ID=MsG0580025574.01.T01:three_prime_utr;Parent=MsG0580025574.01.T01
Gene Sequence

>MsG0580025574.01.T01

ATGAGAAAAACAGGTCGTGAAGGAAAAGAGAAGAAAAGTAGCGGGGGAGTTGTGGTTGAAGGTGGTAGAAAAAGGAGAAGAAAGCACGGTGGTGATGGAATTGAGGAAGGGAGGAAGAAAGAAGAGATTGAAGGTGGAGTAGAAGGAAAAGGAAGCACGATGCTGGTGGTACCTGGGAAGTCTAAACAAGATTGCTTCGATAGAGTTCACCACGACTTCCACACACCACCTCAATGTCAGCCTCGATCAAGGGCAAAGGCAATTAACTCATCACCCCTTCATCAATTCTCAATATCTCCAAGTAAACTGCTCATACCCACTGGAAAGAAGGTTGGCAAATCAAATATTCTAAAACCAAAGAGCATTGTTACTCAGAAGTCTATTGAGAATCTATTACAGTGCCATCTTAAAGTTGATCAAAATCATAAAGGGGACATATTTTCTGTTCTTGAACCCAACTTTGATTTTTCTACTAATGCTATTCTGCCTAGTCAAGAACTTTGTACTCCAAAGCAACAAAAGGAAAATCAAGGGTTCCTACAAATTTGCAATGAAAGATCATCATCATCTTCAATTCATAAGAAGTCTCTTTCTAGATTTAGTGGCTCATCAGGTGTTAAAGATCTTGCTAGTCCACCTGTGCTAAAGCAAGTAAAAAACAAGGTGCAGCATGAAAAATATGTCAATCAATTAAGATTTAGGGACCTTAGGAGAAGAGCAGCATCTTCAAGGGCAAAAAAATCTGTAGCTGGAGAAGGAAAAGGCATTCAAAAAACAGATGTTGTTAAAGCAGCAAAAGTTGCATTGGTTTCTGAAGCTAGAGATGCTATCAATAAATTTCAGCAATCCAAATCAATTTCATGGGCGACACTTGTAGTTCTGATGAGGACAATGACGATGACACTGGAGCTGAAGATGAAACTCCATAGGCTTTTCTCGAGCTAA
Protein sequence

>MsG0580025574.01.T01

MRKTGREGKEKKSSGGVVVEGGRKRRRKHGGDGIEEGRKKEEIEGGVEGKGSTMLVVPGKSKQDCFDRVHHDFHTPPQCQPRSRAKAINSSPLHQFSISPSKLLIPTGKKVGKSNILKPKSIVTQKSIENLLQCHLKVDQNHKGDIFSVLEPNFDFSTNAILPSQELCTPKQQKENQGFLQICNERSSSSSIHKKSLSRFSGSSGVKDLASPPVLKQVKNKVQHEKYVNQLRFRDLRRRAASSRAKKSVAGEGKGIQKTDVVKAAKVALVSEARDAINKFQQSKSISWATLVVLMRTMTMTLELKMKLHRLFSS*