Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002071.01 | MsG0580025637.01 | 0.801134 | 1.038866e-48 | 4.923676e-46 |
| MsG0480022169.01 | MsG0580025637.01 | 0.808977 | 2.375499e-50 | 1.377630e-47 |
| MsG0580025637.01 | MsG0580025638.01 | 0.806838 | 6.771861e-50 | 3.713608e-47 |
| MsG0580025637.01 | MsG0780040788.01 | 0.817602 | 3.028534e-52 | 2.215418e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTACCTTGATTATTAAT+TGG | 0.214897 | 5:+21609842 | MsG0580025637.01.T01:CDS |
| AAGTACCTTTATTGGACTTC+TGG | 0.253983 | 5:+21609876 | MsG0580025637.01.T01:CDS |
| TAACAGTGAACTTAAGTGAT+AGG | 0.342394 | 5:-21609601 | None:intergenic |
| GCATTATTCCTTACCAAGGT+TGG | 0.393911 | 5:-21609723 | None:intergenic |
| AGCATCTTCCACTCTGCAGC+TGG | 0.428640 | 5:+21609690 | MsG0580025637.01.T01:CDS |
| ACACACCAGAAGTCCAATAA+AGG | 0.428862 | 5:-21609881 | None:intergenic |
| TTGTGTGCAGGAATCTCAAA+AGG | 0.437894 | 5:+21609637 | MsG0580025637.01.T01:CDS |
| TAAACTAAATTATTGTGTGC+AGG | 0.462380 | 5:+21609625 | MsG0580025637.01.T01:CDS |
| CATGTTCCAATTAATAATCA+AGG | 0.501738 | 5:-21609848 | None:intergenic |
| TGAACTTAAGTGATAGGTTG+AGG | 0.511110 | 5:-21609595 | None:intergenic |
| TGGAATCAGACAACCAACCT+TGG | 0.592819 | 5:+21609710 | MsG0580025637.01.T01:CDS |
| TGTGTGCAGGAATCTCAAAA+GGG | 0.637356 | 5:+21609638 | MsG0580025637.01.T01:CDS |
| GGGTGTATTAAGCAGAACGT+TGG | 0.667674 | 5:+21609658 | MsG0580025637.01.T01:CDS |
| GTCTGATTCCAGCTGCAGAG+TGG | 0.668776 | 5:-21609698 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAATGTAGAGATAAATTT+TGG | - | Chr5:21609748-21609767 | None:intergenic | 15.0% |
| !! | CTCTACATTAAAAAGTTTAA+TGG | + | Chr5:21609756-21609775 | MsG0580025637.01.T01:intron | 20.0% |
| !! | TCATTACCTTGATTATTAAT+TGG | + | Chr5:21609842-21609861 | MsG0580025637.01.T01:CDS | 20.0% |
| ! | CATGTTCCAATTAATAATCA+AGG | - | Chr5:21609851-21609870 | None:intergenic | 25.0% |
| ! | TAAACTAAATTATTGTGTGC+AGG | + | Chr5:21609625-21609644 | MsG0580025637.01.T01:CDS | 25.0% |
| !!! | ATGCTTTTAAGTACCTTTAT+TGG | + | Chr5:21609868-21609887 | MsG0580025637.01.T01:CDS | 25.0% |
| CAGATTGCATAAAATGCATA+AGG | - | Chr5:21609808-21609827 | None:intergenic | 30.0% | |
| TAACAGTGAACTTAAGTGAT+AGG | - | Chr5:21609604-21609623 | None:intergenic | 30.0% | |
| AAGTACCTTTATTGGACTTC+TGG | + | Chr5:21609876-21609895 | MsG0580025637.01.T01:CDS | 35.0% | |
| TGAACTTAAGTGATAGGTTG+AGG | - | Chr5:21609598-21609617 | None:intergenic | 35.0% | |
| TTTGGCATTATTCCTTACCA+AGG | - | Chr5:21609730-21609749 | None:intergenic | 35.0% | |
| ! | CATTTTATGCAATCTGTGCA+TGG | + | Chr5:21609812-21609831 | MsG0580025637.01.T01:intron | 35.0% |
| ACACACCAGAAGTCCAATAA+AGG | - | Chr5:21609884-21609903 | None:intergenic | 40.0% | |
| GCATTATTCCTTACCAAGGT+TGG | - | Chr5:21609726-21609745 | None:intergenic | 40.0% | |
| TGTGTGCAGGAATCTCAAAA+GGG | + | Chr5:21609638-21609657 | MsG0580025637.01.T01:CDS | 40.0% | |
| TTGTGTGCAGGAATCTCAAA+AGG | + | Chr5:21609637-21609656 | MsG0580025637.01.T01:CDS | 40.0% | |
| GGGTGTATTAAGCAGAACGT+TGG | + | Chr5:21609658-21609677 | MsG0580025637.01.T01:CDS | 45.0% | |
| TCAGACAACCAACCTTGGTA+AGG | + | Chr5:21609715-21609734 | MsG0580025637.01.T01:intron | 45.0% | |
| TGGAATCAGACAACCAACCT+TGG | + | Chr5:21609710-21609729 | MsG0580025637.01.T01:CDS | 45.0% | |
| AGCATCTTCCACTCTGCAGC+TGG | + | Chr5:21609690-21609709 | MsG0580025637.01.T01:CDS | 55.0% | |
| GTCTGATTCCAGCTGCAGAG+TGG | - | Chr5:21609701-21609720 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 21609587 | 21609927 | 21609587 | ID=MsG0580025637.01;Name=MsG0580025637.01 |
| Chr5 | mRNA | 21609587 | 21609927 | 21609587 | ID=MsG0580025637.01.T01;Parent=MsG0580025637.01;Name=MsG0580025637.01.T01;_AED=0.50;_eAED=0.67;_QI=0|0|0|1|0|0|2|0|82 |
| Chr5 | exon | 21609587 | 21609731 | 21609587 | ID=MsG0580025637.01.T01:exon:9201;Parent=MsG0580025637.01.T01 |
| Chr5 | exon | 21609824 | 21609927 | 21609824 | ID=MsG0580025637.01.T01:exon:9202;Parent=MsG0580025637.01.T01 |
| Chr5 | CDS | 21609587 | 21609731 | 21609587 | ID=MsG0580025637.01.T01:cds;Parent=MsG0580025637.01.T01 |
| Chr5 | CDS | 21609824 | 21609927 | 21609824 | ID=MsG0580025637.01.T01:cds;Parent=MsG0580025637.01.T01 |
| Gene Sequence |
| Protein sequence |
