AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040788.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040788.01.T01 MTR_0042s0100 46.569 204 79 5 1 201 20 196 4.13e-41 140
MsG0780040788.01.T01 MTR_5g053815 52.201 159 64 4 83 229 105 263 1.89e-40 140
MsG0780040788.01.T01 MTR_3g012210 40.417 240 100 5 1 201 121 356 5.29e-31 119
MsG0780040788.01.T01 MTR_8g463500 37.589 141 63 3 1 117 268 407 1.94e-22 95.5
MsG0780040788.01.T01 MTR_1g053420 35.758 165 86 5 1 149 116 276 1.83e-20 88.2
MsG0780040788.01.T01 MTR_6g464680 35.200 125 65 2 1 122 241 352 1.55e-17 81.3
MsG0780040788.01.T01 MTR_3g435760 79.545 44 9 0 1 44 93 136 1.77e-17 77.0
MsG0780040788.01.T01 MTR_7g050295 39.362 94 54 2 56 149 20 110 1.10e-13 66.2
MsG0780040788.01.T01 MTR_1g060170 55.769 52 23 0 1 52 86 137 3.36e-13 66.6
MsG0780040788.01.T01 MTR_0150s0040 42.683 82 46 1 41 122 14 94 3.60e-13 67.4
MsG0780040788.01.T01 MTR_0034s0110 46.552 58 31 0 56 113 59 116 5.69e-13 64.3
MsG0780040788.01.T01 MTR_8g106890 38.158 76 44 1 69 144 10 82 7.17e-13 63.2
MsG0780040788.01.T01 MTR_2g460920 40.580 69 41 0 56 124 19 87 1.52e-11 61.2
MsG0780040788.01.T01 MTR_1589s0010 34.043 94 59 1 56 149 183 273 3.78e-11 62.0
MsG0780040788.01.T01 MTR_2g461440 35.484 93 57 1 56 148 140 229 4.48e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATCCTTGACAATAAGTA+TGG 0.295692 7:+82435960 MsG0780040788.01.T01:CDS
TGAAAACTACCTGCTCCTTA+TGG 0.300654 7:-82435707 None:intergenic
ATCCCCTTTGGAATGCATAA+TGG 0.337553 7:+82436056 MsG0780040788.01.T01:CDS
AATCAAGAACTTAATGCTTT+CGG 0.338998 7:-82436107 None:intergenic
AACACTTGTGGTTATCATCT+TGG 0.364687 7:-82435752 None:intergenic
GAGGATATGCCACTCATTCT+AGG 0.368548 7:+82435618 MsG0780040788.01.T01:CDS
TGTTAAGTCTATTGAAAGTA+AGG 0.374828 7:+82435854 MsG0780040788.01.T01:CDS
GTTGAATAGTTTGAGATTCA+AGG 0.377305 7:-82435815 None:intergenic
AATGCTTTCGGACATTTAAT+CGG 0.380344 7:-82436095 None:intergenic
ATGATAACCACAAGTGTTAC+CGG 0.408361 7:+82435757 MsG0780040788.01.T01:CDS
TCCCCTTTGGAATGCATAAT+GGG 0.416183 7:+82436057 MsG0780040788.01.T01:CDS
CTTTCGGACATTTAATCGGT+TGG 0.427528 7:-82436091 None:intergenic
GGGTTAGTGAAAATAGTCTT+AGG 0.428731 7:-82436008 None:intergenic
GGAAGACCTTTCCTTGCAAC+TGG 0.430802 7:+82435639 MsG0780040788.01.T01:CDS
TTGAATAGTTTGAGATTCAA+GGG 0.435558 7:-82435814 None:intergenic
AGGAAGTGGTTGAAGATGTT+GGG 0.439621 7:+82435874 MsG0780040788.01.T01:CDS
AAGGAAGTGGTTGAAGATGT+TGG 0.441462 7:+82435873 MsG0780040788.01.T01:CDS
GCTTATAAGAAGATCCCCTT+TGG 0.446886 7:+82436044 MsG0780040788.01.T01:CDS
TCTAAGACCACAAAATCTGC+TGG 0.448725 7:-82435582 None:intergenic
CATTTACTATCCCTTGTTCA+AGG 0.453761 7:+82435366 MsG0780040788.01.T01:CDS
TTTCGGACATTTAATCGGTT+GGG 0.456318 7:-82436090 None:intergenic
ATCTCAAACTATTCAACCTA+TGG 0.456995 7:+82435821 MsG0780040788.01.T01:CDS
TTCATGAACTTAGCATATAA+CGG 0.461920 7:-82435230 None:intergenic
GTTAATGTTGAGGTTCCATA+AGG 0.475660 7:+82435692 MsG0780040788.01.T01:CDS
GTCTATTGAAAGTAAGGAAG+TGG 0.479583 7:+82435860 MsG0780040788.01.T01:CDS
CCACCTAAGCTGAAGGATCC+TGG 0.480044 7:+82435341 MsG0780040788.01.T01:CDS
TTATAAGCAAACACACTACA+AGG 0.485213 7:-82436029 None:intergenic
GCAATGTTCTCATCATCCTT+AGG 0.490883 7:-82435278 None:intergenic
GCACCAGGATCCTTCAGCTT+AGG 0.499980 7:-82435344 None:intergenic
TTAACAATTTCTCCTTCCAT+AGG 0.504186 7:-82435837 None:intergenic
GGCCCATTATGCATTCCAAA+GGG 0.508175 7:-82436059 None:intergenic
CTTATGCAACAACAATCATC+AGG 0.508443 7:+82435936 MsG0780040788.01.T01:CDS
TTTCTTCACTAACGTCAATC+CGG 0.511135 7:-82435776 None:intergenic
CCAGGATCCTTCAGCTTAGG+TGG 0.532915 7:-82435341 None:intergenic
TGTCTACCGGTCCTTGAACA+AGG 0.534133 7:-82435377 None:intergenic
ACTATCCCTTGTTCAAGGAC+CGG 0.539995 7:+82435371 MsG0780040788.01.T01:CDS
CTTAGATATGGCTGAAGATG+AGG 0.553355 7:+82435599 MsG0780040788.01.T01:CDS
GTTGAGGTTCCATAAGGAGC+AGG 0.558031 7:+82435698 MsG0780040788.01.T01:CDS
CGGCCCATTATGCATTCCAA+AGG 0.571380 7:-82436060 None:intergenic
CTAAGACCACAAAATCTGCT+GGG 0.579911 7:-82435581 None:intergenic
ACATCCTTGACAATAAGTAT+GGG 0.582201 7:+82435961 MsG0780040788.01.T01:CDS
GCACGACCAGTTGCAAGGAA+AGG 0.586185 7:-82435645 None:intergenic
GTCTACCGGTCCTTGAACAA+GGG 0.586845 7:-82435376 None:intergenic
GAAGCTGCCACCTAAGCTGA+AGG 0.591921 7:+82435334 MsG0780040788.01.T01:CDS
CAAGGGATAGTAAATGCACC+AGG 0.594756 7:-82435359 None:intergenic
CGTCAATCCGGTAACACTTG+TGG 0.600458 7:-82435764 None:intergenic
CATCCTTGACAATAAGTATG+GGG 0.600898 7:+82435962 MsG0780040788.01.T01:CDS
CAAACTATTCAACCTATGGA+AGG 0.601965 7:+82435825 MsG0780040788.01.T01:CDS
GACTAAACGAAGGAAGCCTA+AGG 0.604111 7:+82435262 MsG0780040788.01.T01:CDS
GGAAGTGGTTGAAGATGTTG+GGG 0.613260 7:+82435875 MsG0780040788.01.T01:CDS
AAAGGTCTTCCTAGAATGAG+TGG 0.620781 7:-82435627 None:intergenic
GCTAGTCAAGGTTAATGACT+TGG 0.622841 7:+82435554 MsG0780040788.01.T01:CDS
TTAAAGCACGACCAGTTGCA+AGG 0.628228 7:-82435650 None:intergenic
ATCCTTGACAATAAGTATGG+GGG 0.660705 7:+82435963 MsG0780040788.01.T01:CDS
TATAAGCAAACACACTACAA+GGG 0.664352 7:-82436028 None:intergenic
GCTTTAATTGATGTTGAACG+TGG 0.665918 7:+82435666 MsG0780040788.01.T01:CDS
TTAATCGGTTGGGAAATCGT+CGG 0.686173 7:-82436080 None:intergenic
AACGTGGAGAGTTAATGTTG+AGG 0.688683 7:+82435682 MsG0780040788.01.T01:CDS
GCTTGAAGATGTGCTAGTCA+AGG 0.699656 7:+82435542 MsG0780040788.01.T01:intron
GCCCATTATGCATTCCAAAG+GGG 0.704898 7:-82436058 None:intergenic
AAGACTTGTTGACTAAACGA+AGG 0.710692 7:+82435252 MsG0780040788.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGTTAAGTCTATTGAAAGTA+AGG + Chr7:82435854-82435873 MsG0780040788.01.T01:CDS 25.0%
! TTCATGAACTTAGCATATAA+CGG - Chr7:82435233-82435252 None:intergenic 25.0%
!! TTGAATAGTTTGAGATTCAA+GGG - Chr7:82435817-82435836 None:intergenic 25.0%
AAAGTAATGGTCATTCTTGT+TGG - Chr7:82435489-82435508 None:intergenic 30.0%
AATGCTTTCGGACATTTAAT+CGG - Chr7:82436098-82436117 None:intergenic 30.0%
ACATCCTTGACAATAAGTAT+GGG + Chr7:82435961-82435980 MsG0780040788.01.T01:CDS 30.0%
ATCTCAAACTATTCAACCTA+TGG + Chr7:82435821-82435840 MsG0780040788.01.T01:CDS 30.0%
TATAAGCAAACACACTACAA+GGG - Chr7:82436031-82436050 None:intergenic 30.0%
TGACCATTACTTTAGTTGAT+CGG + Chr7:82435496-82435515 MsG0780040788.01.T01:intron 30.0%
TTAACAATTTCTCCTTCCAT+AGG - Chr7:82435840-82435859 None:intergenic 30.0%
TTATAAGCAAACACACTACA+AGG - Chr7:82436032-82436051 None:intergenic 30.0%
! GGTAGTTTTCAATATCTTTG+AGG + Chr7:82435719-82435738 MsG0780040788.01.T01:CDS 30.0%
! GTTGAATAGTTTGAGATTCA+AGG - Chr7:82435818-82435837 None:intergenic 30.0%
!! AGATTTTGTGGTCTTAGATA+TGG + Chr7:82435587-82435606 MsG0780040788.01.T01:CDS 30.0%
AACACTTGTGGTTATCATCT+TGG - Chr7:82435755-82435774 None:intergenic 35.0%
AGCTTCTTCATCATTGAAAG+AGG - Chr7:82435447-82435466 None:intergenic 35.0%
ATCCTTGACAATAAGTATGG+GGG + Chr7:82435963-82435982 MsG0780040788.01.T01:CDS 35.0%
ATGATAACCACAAGTGTTAC+CGG + Chr7:82435757-82435776 MsG0780040788.01.T01:CDS 35.0%
ATGATGAAGAAGCTTAAGTG+TGG + Chr7:82435453-82435472 MsG0780040788.01.T01:intron 35.0%
CAAACTATTCAACCTATGGA+AGG + Chr7:82435825-82435844 MsG0780040788.01.T01:CDS 35.0%
CATCCTTGACAATAAGTATG+GGG + Chr7:82435962-82435981 MsG0780040788.01.T01:CDS 35.0%
CATTTACTATCCCTTGTTCA+AGG + Chr7:82435366-82435385 MsG0780040788.01.T01:CDS 35.0%
CTTATGCAACAACAATCATC+AGG + Chr7:82435936-82435955 MsG0780040788.01.T01:CDS 35.0%
GACATCCTTGACAATAAGTA+TGG + Chr7:82435960-82435979 MsG0780040788.01.T01:CDS 35.0%
GCTTTAATTGATGTTGAACG+TGG + Chr7:82435666-82435685 MsG0780040788.01.T01:CDS 35.0%
GTCTATTGAAAGTAAGGAAG+TGG + Chr7:82435860-82435879 MsG0780040788.01.T01:CDS 35.0%
GTTAATGTTGAGGTTCCATA+AGG + Chr7:82435692-82435711 MsG0780040788.01.T01:CDS 35.0%
TGACCGATCAACTAAAGTAA+TGG - Chr7:82435502-82435521 None:intergenic 35.0%
TGATGAAGAAGCTTAAGTGT+GGG + Chr7:82435454-82435473 MsG0780040788.01.T01:intron 35.0%
TTTCGGACATTTAATCGGTT+GGG - Chr7:82436093-82436112 None:intergenic 35.0%
TTTCTTCACTAACGTCAATC+CGG - Chr7:82435779-82435798 None:intergenic 35.0%
! GGGTTAGTGAAAATAGTCTT+AGG - Chr7:82436011-82436030 None:intergenic 35.0%
!! AAGACTTGTTGACTAAACGA+AGG + Chr7:82435252-82435271 MsG0780040788.01.T01:CDS 35.0%
!!! ATGGTCATTCTTGTTGGTTT+TGG - Chr7:82435483-82435502 None:intergenic 35.0%
AAAGCTCTTCCAATGTCTAC+CGG - Chr7:82435393-82435412 None:intergenic 40.0%
AAAGGTCTTCCTAGAATGAG+TGG - Chr7:82435630-82435649 None:intergenic 40.0%
AACGTGGAGAGTTAATGTTG+AGG + Chr7:82435682-82435701 MsG0780040788.01.T01:CDS 40.0%
AAGGAAGTGGTTGAAGATGT+TGG + Chr7:82435873-82435892 MsG0780040788.01.T01:CDS 40.0%
AGGAAGTGGTTGAAGATGTT+GGG + Chr7:82435874-82435893 MsG0780040788.01.T01:CDS 40.0%
ATCCCCCATACTTATTGTCA+AGG - Chr7:82435968-82435987 None:intergenic 40.0%
CGGTCAATCTCTTATCCTTT+CGG + Chr7:82435516-82435535 MsG0780040788.01.T01:intron 40.0%
CTAAGACCACAAAATCTGCT+GGG - Chr7:82435584-82435603 None:intergenic 40.0%
CTTAGATATGGCTGAAGATG+AGG + Chr7:82435599-82435618 MsG0780040788.01.T01:CDS 40.0%
CTTTCGGACATTTAATCGGT+TGG - Chr7:82436094-82436113 None:intergenic 40.0%
GACAATAAGTATGGGGGATT+TGG + Chr7:82435969-82435988 MsG0780040788.01.T01:CDS 40.0%
GATGAAGAAGCTTAAGTGTG+GGG + Chr7:82435455-82435474 MsG0780040788.01.T01:intron 40.0%
GCAATGTTCTCATCATCCTT+AGG - Chr7:82435281-82435300 None:intergenic 40.0%
GCTAGTCAAGGTTAATGACT+TGG + Chr7:82435554-82435573 MsG0780040788.01.T01:CDS 40.0%
GCTTATAAGAAGATCCCCTT+TGG + Chr7:82436044-82436063 MsG0780040788.01.T01:CDS 40.0%
TGAAAACTACCTGCTCCTTA+TGG - Chr7:82435710-82435729 None:intergenic 40.0%
TTAATCGGTTGGGAAATCGT+CGG - Chr7:82436083-82436102 None:intergenic 40.0%
! GGAAGAGCTTTATGTGACTT+GGG + Chr7:82435402-82435421 MsG0780040788.01.T01:intron 40.0%
! TCTAAGACCACAAAATCTGC+TGG - Chr7:82435585-82435604 None:intergenic 40.0%
! TGGAAGAGCTTTATGTGACT+TGG + Chr7:82435401-82435420 MsG0780040788.01.T01:intron 40.0%
!! ATCCCCTTTGGAATGCATAA+TGG + Chr7:82436056-82436075 MsG0780040788.01.T01:CDS 40.0%
!! TCCCCTTTGGAATGCATAAT+GGG + Chr7:82436057-82436076 MsG0780040788.01.T01:CDS 40.0%
ACATCTTCAAGCACACCGAA+AGG - Chr7:82435534-82435553 None:intergenic 45.0%
ACTATCCCTTGTTCAAGGAC+CGG + Chr7:82435371-82435390 MsG0780040788.01.T01:CDS 45.0%
AGTATGGGGGATTTGGAAGT+TGG + Chr7:82435976-82435995 MsG0780040788.01.T01:CDS 45.0%
CAAGGGATAGTAAATGCACC+AGG - Chr7:82435362-82435381 None:intergenic 45.0%
GACTAAACGAAGGAAGCCTA+AGG + Chr7:82435262-82435281 MsG0780040788.01.T01:CDS 45.0%
GAGGATATGCCACTCATTCT+AGG + Chr7:82435618-82435637 MsG0780040788.01.T01:CDS 45.0%
GCCCATTATGCATTCCAAAG+GGG - Chr7:82436061-82436080 None:intergenic 45.0%
GCTTGAAGATGTGCTAGTCA+AGG + Chr7:82435542-82435561 MsG0780040788.01.T01:intron 45.0%
GGAAGTGGTTGAAGATGTTG+GGG + Chr7:82435875-82435894 MsG0780040788.01.T01:CDS 45.0%
GGCCCATTATGCATTCCAAA+GGG - Chr7:82436062-82436081 None:intergenic 45.0%
TTAAAGCACGACCAGTTGCA+AGG - Chr7:82435653-82435672 None:intergenic 45.0%
CGGCCCATTATGCATTCCAA+AGG - Chr7:82436063-82436082 None:intergenic 50.0%
CGTCAATCCGGTAACACTTG+TGG - Chr7:82435767-82435786 None:intergenic 50.0%
GGAAGACCTTTCCTTGCAAC+TGG + Chr7:82435639-82435658 MsG0780040788.01.T01:CDS 50.0%
GTCTACCGGTCCTTGAACAA+GGG - Chr7:82435379-82435398 None:intergenic 50.0%
GTTCAAGGACCGGTAGACAT+TGG + Chr7:82435381-82435400 MsG0780040788.01.T01:intron 50.0%
GTTGAGGTTCCATAAGGAGC+AGG + Chr7:82435698-82435717 MsG0780040788.01.T01:CDS 50.0%
TGTCTACCGGTCCTTGAACA+AGG - Chr7:82435380-82435399 None:intergenic 50.0%
! GGTGTTCCCAGCAGATTTTG+TGG + Chr7:82435575-82435594 MsG0780040788.01.T01:CDS 50.0%
CCACCTAAGCTGAAGGATCC+TGG + Chr7:82435341-82435360 MsG0780040788.01.T01:CDS 55.0%
CCAGGATCCTTCAGCTTAGG+TGG - Chr7:82435344-82435363 None:intergenic 55.0%
GAAGCTGCCACCTAAGCTGA+AGG + Chr7:82435334-82435353 MsG0780040788.01.T01:CDS 55.0%
GCACCAGGATCCTTCAGCTT+AGG - Chr7:82435347-82435366 None:intergenic 55.0%
GCACGACCAGTTGCAAGGAA+AGG - Chr7:82435648-82435667 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 82435227 82436127 82435227 ID=MsG0780040788.01;Name=MsG0780040788.01
Chr7 mRNA 82435227 82436127 82435227 ID=MsG0780040788.01.T01;Parent=MsG0780040788.01;Name=MsG0780040788.01.T01;_AED=0.49;_eAED=0.63;_QI=0|0|0|1|1|1|2|0|247
Chr7 exon 82435227 82435392 82435227 ID=MsG0780040788.01.T01:exon:38329;Parent=MsG0780040788.01.T01
Chr7 exon 82435550 82436127 82435550 ID=MsG0780040788.01.T01:exon:38330;Parent=MsG0780040788.01.T01
Chr7 CDS 82435227 82435392 82435227 ID=MsG0780040788.01.T01:cds;Parent=MsG0780040788.01.T01
Chr7 CDS 82435550 82436127 82435550 ID=MsG0780040788.01.T01:cds;Parent=MsG0780040788.01.T01
Gene Sequence

>MsG0780040788.01.T01

ATGCCGTTATATGCTAAGTTCATGAAAGACTTGTTGACTAAACGAAGGAAGCCTAAGGATGATGAGAACATTGCACTTTCTGAAAACTGTAGTGCTATTCTTCAAAAGAAGCTGCCACCTAAGCTGAAGGATCCTGGTGCATTTACTATCCCTTGTTCAAGGACCGATGTGCTAGTCAAGGTTAATGACTTGGTGTTCCCAGCAGATTTTGTGGTCTTAGATATGGCTGAAGATGAGGATATGCCACTCATTCTAGGAAGACCTTTCCTTGCAACTGGTCGTGCTTTAATTGATGTTGAACGTGGAGAGTTAATGTTGAGGTTCCATAAGGAGCAGGTAGTTTTCAATATCTTTGAGGCAATAAAGAACCAAGATGATAACCACAAGTGTTACCGGATTGACGTTAGTGAAGAAACAGTCGAAGAAAACTCCCTTGAATCTCAAACTATTCAACCTATGGAAGGAGAAATTGTTAAGTCTATTGAAAGTAAGGAAGTGGTTGAAGATGTTGGGGTGACAGAGTGTGTCAAGCAACTTGAAGTAAGCACAGAACTTATGCAACAACAATCATCAGGAGACATCCTTGACAATAAGTATGGGGGATTTGGAAGTTGGAACAACATGCCTAAGACTATTTTCACTAACCCTTGTAGTGTGTTTGCTTATAAGAAGATCCCCTTTGGAATGCATAATGGGCCGACGATTTCCCAACCGATTAAATGTCCGAAAGCATTAAGTTCTTGA

Protein sequence

>MsG0780040788.01.T01

MPLYAKFMKDLLTKRRKPKDDENIALSENCSAILQKKLPPKLKDPGAFTIPCSRTDVLVKVNDLVFPADFVVLDMAEDEDMPLILGRPFLATGRALIDVERGELMLRFHKEQVVFNIFEAIKNQDDNHKCYRIDVSEETVEENSLESQTIQPMEGEIVKSIESKEVVEDVGVTECVKQLEVSTELMQQQSSGDILDNKYGGFGSWNNMPKTIFTNPCSVFAYKKIPFGMHNGPTISQPIKCPKALSS*