AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025833.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025833.01.T01 MTR_5g035090 97.514 362 9 0 21 382 73 434 0.0 747
MsG0580025833.01.T01 MTR_5g035070 74.728 368 90 3 22 386 77 444 0.0 575
MsG0580025833.01.T01 MTR_5g035150 70.141 355 104 2 27 379 81 435 0.0 530
MsG0580025833.01.T01 MTR_5g035080 65.160 376 120 5 18 386 62 433 9.02e-180 506
MsG0580025833.01.T01 MTR_3g109840 61.749 366 130 5 21 382 63 422 1.24e-168 478
MsG0580025833.01.T01 MTR_8g104970 60.278 360 134 5 22 376 111 466 2.47e-160 459
MsG0580025833.01.T01 MTR_7g033230 56.757 370 148 6 13 377 249 611 1.24e-151 442
MsG0580025833.01.T01 MTR_7g033230 56.757 370 148 6 13 377 319 681 9.89e-151 442
MsG0580025833.01.T01 MTR_5g035140 61.132 265 94 5 118 377 7 267 3.12e-117 342
MsG0580025833.01.T01 MTR_6g016880 42.424 363 190 9 31 385 78 429 9.19e-103 311
MsG0580025833.01.T01 MTR_8g027900 43.182 352 188 7 30 375 210 555 1.45e-100 309
MsG0580025833.01.T01 MTR_8g099300 42.493 353 190 8 30 375 73 419 1.41e-97 297
MsG0580025833.01.T01 MTR_7g019120 39.669 363 202 9 30 386 78 429 1.01e-95 293
MsG0580025833.01.T01 MTR_6g007777 38.420 367 211 8 23 383 108 465 2.07e-89 278
MsG0580025833.01.T01 MTR_6g016875 40.556 360 198 9 25 376 58 409 4.39e-87 270
MsG0580025833.01.T01 MTR_2g016060 39.554 359 192 12 32 377 56 402 2.33e-82 257
MsG0580025833.01.T01 MTR_1g076690 36.856 369 202 10 26 377 218 572 3.11e-72 236
MsG0580025833.01.T01 MTR_6g061710 34.309 376 214 10 20 377 156 516 2.24e-68 224
MsG0580025833.01.T01 MTR_7g094750 31.967 366 212 15 29 377 89 434 2.56e-51 178
MsG0580025833.01.T01 MTR_2g022560 33.162 389 218 14 10 379 76 441 2.81e-51 178
MsG0580025833.01.T01 MTR_0136s0010 33.152 368 202 16 30 377 169 512 1.76e-46 166
MsG0580025833.01.T01 MTR_1g058380 32.329 365 207 14 31 377 55 397 2.35e-46 164
MsG0580025833.01.T01 MTR_8g093530 31.367 373 212 12 26 385 85 426 1.53e-45 162
MsG0580025833.01.T01 MTR_4g126950 33.333 360 205 14 30 375 108 446 5.43e-45 161
MsG0580025833.01.T01 MTR_5g071350 29.670 364 227 10 21 371 48 395 2.95e-44 158
MsG0580025833.01.T01 MTR_7g093450 32.312 359 204 13 30 371 247 583 2.99e-44 162
MsG0580025833.01.T01 MTR_3g464570 31.564 358 206 14 28 371 40 372 1.38e-42 153
MsG0580025833.01.T01 MTR_4g014440 30.874 366 216 13 29 375 60 407 2.79e-42 153
MsG0580025833.01.T01 MTR_5g035120 59.804 102 41 0 20 121 64 165 5.72e-42 145
MsG0580025833.01.T01 MTR_8g069790 29.620 368 217 14 29 378 176 519 7.59e-42 154
MsG0580025833.01.T01 MTR_4g121560 28.108 370 215 13 29 375 112 453 3.65e-41 151
MsG0580025833.01.T01 MTR_5g059400 30.343 379 220 15 28 384 172 528 1.78e-40 150
MsG0580025833.01.T01 MTR_1g116130 30.343 379 220 15 28 384 172 528 1.78e-40 150
MsG0580025833.01.T01 MTR_4g125060 30.971 381 215 14 19 375 115 471 3.27e-40 149
MsG0580025833.01.T01 MTR_5g033800 27.882 373 227 12 23 377 137 485 3.92e-40 149
MsG0580025833.01.T01 MTR_7g070060 30.055 366 219 14 29 375 62 409 6.08e-40 147
MsG0580025833.01.T01 MTR_4g125060 30.971 381 215 14 19 375 154 510 6.82e-40 148
MsG0580025833.01.T01 MTR_4g125060 30.971 381 215 14 19 375 183 539 8.98e-40 149
MsG0580025833.01.T01 MTR_2g091175 30.812 357 201 16 24 371 43 362 1.57e-38 142
MsG0580025833.01.T01 MTR_4g016940 28.809 361 224 11 30 375 85 427 1.73e-38 143
MsG0580025833.01.T01 MTR_7g109550 30.704 355 206 14 31 372 137 464 1.77e-38 144
MsG0580025833.01.T01 MTR_7g068920 30.447 358 222 13 30 375 79 421 2.42e-38 142
MsG0580025833.01.T01 MTR_4g088030 30.172 348 208 11 28 371 30 346 4.87e-38 140
MsG0580025833.01.T01 MTR_3g021060 30.726 358 201 17 30 377 45 365 5.84e-38 140
MsG0580025833.01.T01 MTR_3g070900 29.348 368 211 15 28 371 103 445 7.77e-38 142
MsG0580025833.01.T01 MTR_4g087730 29.661 354 214 12 22 371 36 358 2.35e-37 139
MsG0580025833.01.T01 MTR_2g073510 29.716 387 221 15 4 375 3 353 2.62e-37 138
MsG0580025833.01.T01 MTR_3g090120 28.684 380 223 12 18 380 116 464 3.19e-37 140
MsG0580025833.01.T01 MTR_3g035830 29.692 357 202 12 30 375 30 348 3.84e-37 138
MsG0580025833.01.T01 MTR_8g093870 28.947 380 215 14 21 375 123 472 5.00e-37 140
MsG0580025833.01.T01 MTR_3g021090 30.685 365 192 15 24 371 24 344 1.40e-36 137
MsG0580025833.01.T01 MTR_8g463790 27.901 362 213 12 31 371 71 405 1.80e-36 137
MsG0580025833.01.T01 MTR_7g094750 33.597 253 143 9 29 267 89 330 9.10e-36 134
MsG0580025833.01.T01 MTR_5g083260 28.169 355 226 12 31 372 83 421 1.02e-35 135
MsG0580025833.01.T01 MTR_1g050448 29.223 373 214 16 17 375 39 375 1.30e-35 134
MsG0580025833.01.T01 MTR_3g071650 28.926 363 213 15 31 372 89 427 4.65e-35 134
MsG0580025833.01.T01 MTR_2g015720 30.423 355 207 14 23 371 34 354 1.64e-34 131
MsG0580025833.01.T01 MTR_3g049330 27.874 348 218 8 28 371 28 346 5.39e-34 129
MsG0580025833.01.T01 MTR_1g112210 26.287 369 223 13 17 371 45 378 9.29e-34 129
MsG0580025833.01.T01 MTR_4g079700 29.143 350 210 13 31 375 47 363 5.16e-33 127
MsG0580025833.01.T01 MTR_2g073500 28.032 371 221 13 10 371 8 341 1.06e-32 125
MsG0580025833.01.T01 MTR_1g112210 25.956 366 215 13 17 371 45 365 8.76e-32 124
MsG0580025833.01.T01 MTR_6g016240 27.100 369 224 12 28 373 68 414 9.80e-32 124
MsG0580025833.01.T01 MTR_4g014430 27.467 375 226 14 21 375 98 446 3.86e-31 123
MsG0580025833.01.T01 MTR_1g111010 29.545 352 207 13 27 375 28 341 5.02e-31 121
MsG0580025833.01.T01 MTR_7g019140 49.451 91 46 0 30 120 78 168 5.44e-31 116
MsG0580025833.01.T01 MTR_5g034810 28.150 373 222 13 9 371 12 348 3.09e-30 119
MsG0580025833.01.T01 MTR_5g082880 27.224 371 225 14 31 377 165 514 1.07e-29 120
MsG0580025833.01.T01 MTR_4g092830 25.134 374 222 13 31 375 48 392 4.27e-29 117
MsG0580025833.01.T01 MTR_5g033800 28.319 226 141 4 23 236 137 353 3.32e-27 111
MsG0580025833.01.T01 MTR_7g070060 33.871 186 109 6 28 204 61 241 1.30e-25 105
MsG0580025833.01.T01 MTR_4g102570 37.419 155 84 5 147 294 2 150 7.78e-23 94.0
MsG0580025833.01.T01 MTR_7g070060 28.617 311 186 13 83 375 15 307 6.74e-22 95.5
MsG0580025833.01.T01 MTR_3g464570 29.310 290 166 14 96 371 4 268 6.00e-21 92.0
MsG0580025833.01.T01 MTR_7g117160 31.336 217 93 8 158 374 29 189 6.90e-20 87.0
MsG0580025833.01.T01 MTR_7g019130 32.031 128 78 4 149 270 1 125 3.00e-17 78.6
MsG0580025833.01.T01 MTR_0929s0010 52.000 50 24 0 30 79 78 127 2.42e-14 69.7
MsG0580025833.01.T01 MTR_5g069320 23.826 298 163 12 77 368 147 386 4.58e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025833.01.T01 AT1G70230 58.120 351 131 5 31 379 79 415 6.38e-156 446
MsG0580025833.01.T01 AT4G01080 43.417 357 187 10 30 381 90 436 9.95e-99 301
MsG0580025833.01.T01 AT1G01430 40.650 369 205 7 24 383 92 455 2.56e-98 300
MsG0580025833.01.T01 AT5G15890 42.450 351 189 7 28 376 184 523 7.67e-97 298
MsG0580025833.01.T01 AT4G23790 41.690 355 193 9 30 375 78 427 1.00e-94 290
MsG0580025833.01.T01 AT5G15900 40.055 362 199 10 28 381 64 415 5.01e-94 288
MsG0580025833.01.T01 AT5G15900 40.055 362 199 10 28 381 83 434 9.47e-94 288
MsG0580025833.01.T01 AT4G11090 42.577 357 185 9 30 375 78 425 7.25e-93 285
MsG0580025833.01.T01 AT3G02440 39.773 352 197 7 31 376 127 469 1.19e-92 286
MsG0580025833.01.T01 AT3G02440 39.773 352 197 7 31 376 135 477 1.34e-92 286
MsG0580025833.01.T01 AT3G28150 39.888 356 194 10 30 377 69 412 2.19e-90 278
MsG0580025833.01.T01 AT4G25360 36.339 366 210 9 26 377 170 526 3.78e-70 229
MsG0580025833.01.T01 AT4G25360 36.339 366 210 9 26 377 170 526 3.78e-70 229
MsG0580025833.01.T01 AT5G51640 34.986 363 216 9 28 377 135 490 1.20e-67 222
MsG0580025833.01.T01 AT5G19160 32.824 393 236 12 10 384 73 455 6.06e-54 185
MsG0580025833.01.T01 AT3G06080 34.584 373 207 16 31 386 103 455 1.02e-52 182
MsG0580025833.01.T01 AT3G12060 32.778 360 201 14 30 371 195 531 1.91e-49 175
MsG0580025833.01.T01 AT1G48880 33.058 363 185 13 29 372 113 436 1.29e-46 165
MsG0580025833.01.T01 AT2G30010 31.707 369 206 13 28 375 53 396 6.53e-46 162
MsG0580025833.01.T01 AT5G06700 31.283 374 215 15 31 385 255 605 1.10e-45 166
MsG0580025833.01.T01 AT2G30010 32.055 365 202 13 28 371 96 435 1.46e-45 162
MsG0580025833.01.T01 AT5G64020 31.267 371 210 14 31 375 57 408 8.41e-45 159
MsG0580025833.01.T01 AT5G20590 33.240 358 199 14 31 371 141 475 2.18e-44 160
MsG0580025833.01.T01 AT5G06230 32.454 379 211 16 15 371 5 360 7.09e-44 156
MsG0580025833.01.T01 AT5G06230 32.454 379 211 16 15 371 46 401 1.26e-43 157
MsG0580025833.01.T01 AT3G11570 31.720 372 204 14 23 371 71 415 1.02e-42 154
MsG0580025833.01.T01 AT2G14530 30.423 378 197 15 31 375 62 406 1.23e-42 154
MsG0580025833.01.T01 AT5G49340 29.248 359 225 11 30 375 99 441 6.47e-41 150
MsG0580025833.01.T01 AT3G14850 29.480 346 210 10 31 371 34 350 9.93e-41 147
MsG0580025833.01.T01 AT5G58600 28.729 362 220 12 28 375 63 400 7.42e-40 146
MsG0580025833.01.T01 AT2G38320 28.342 374 233 14 20 375 48 404 9.96e-40 146
MsG0580025833.01.T01 AT3G62390 29.412 357 224 13 30 377 137 474 2.47e-39 146
MsG0580025833.01.T01 AT3G11030 28.333 360 220 11 31 372 106 445 6.78e-39 145
MsG0580025833.01.T01 AT2G42570 30.110 362 208 16 23 375 39 364 1.33e-38 142
MsG0580025833.01.T01 AT1G60790 28.723 376 209 15 24 371 184 528 4.35e-38 144
MsG0580025833.01.T01 AT2G37720 28.610 367 207 12 31 372 143 479 8.76e-38 142
MsG0580025833.01.T01 AT2G31110 30.000 350 202 15 30 371 44 358 4.17e-37 138
MsG0580025833.01.T01 AT2G40160 28.342 374 217 15 31 377 77 426 6.17e-37 139
MsG0580025833.01.T01 AT2G40160 27.793 367 215 14 31 371 77 419 8.05e-37 139
MsG0580025833.01.T01 AT2G30900 29.155 343 217 8 31 371 43 361 1.14e-36 137
MsG0580025833.01.T01 AT5G19160 34.444 270 155 9 10 264 73 335 1.49e-36 136
MsG0580025833.01.T01 AT1G73140 27.688 372 237 11 13 371 49 401 1.56e-36 137
MsG0580025833.01.T01 AT5G64470 29.839 372 202 16 31 375 55 394 1.69e-36 137
MsG0580025833.01.T01 AT2G34070 28.011 357 225 12 23 376 57 384 2.14e-36 137
MsG0580025833.01.T01 AT5G01360 27.701 361 223 13 30 372 35 375 2.80e-36 136
MsG0580025833.01.T01 AT5G01360 27.701 361 223 13 30 372 91 431 9.79e-36 135
MsG0580025833.01.T01 AT2G40320 28.962 366 215 17 28 372 78 419 1.69e-35 135
MsG0580025833.01.T01 AT5G64470 29.365 378 202 17 31 375 55 400 6.62e-35 133
MsG0580025833.01.T01 AT3G55990 28.919 370 219 14 30 377 139 486 8.51e-35 134
MsG0580025833.01.T01 AT3G06080 35.628 247 138 9 31 265 103 340 3.81e-34 129
MsG0580025833.01.T01 AT5G20680 29.121 364 215 12 31 375 118 457 4.84e-34 131
MsG0580025833.01.T01 AT2G38320 28.698 338 207 13 55 375 1 321 8.39e-34 128
MsG0580025833.01.T01 AT5G20680 28.022 364 219 11 31 375 194 533 1.33e-33 131
MsG0580025833.01.T01 AT5G20680 28.022 364 219 11 31 375 212 551 1.37e-33 131
MsG0580025833.01.T01 AT5G20680 28.022 364 219 11 31 375 212 551 1.37e-33 131
MsG0580025833.01.T01 AT5G20680 28.022 364 219 11 31 375 212 551 1.37e-33 131
MsG0580025833.01.T01 AT5G20680 28.022 364 219 11 31 375 212 551 1.37e-33 131
MsG0580025833.01.T01 AT1G29050 28.571 357 220 13 23 375 53 378 1.88e-33 128
MsG0580025833.01.T01 AT2G40150 27.778 396 230 16 13 377 53 423 3.01e-33 129
MsG0580025833.01.T01 AT3G54260 28.255 361 206 15 31 375 51 374 5.86e-32 124
MsG0580025833.01.T01 AT5G01620 25.683 366 231 13 28 375 107 449 3.72e-31 123
MsG0580025833.01.T01 AT5G01620 25.683 366 231 13 28 375 107 449 3.72e-31 123
MsG0580025833.01.T01 AT5G01620 25.683 366 231 13 28 375 115 457 3.98e-31 123
MsG0580025833.01.T01 AT1G78710 28.997 369 212 16 17 371 20 352 7.54e-31 121
MsG0580025833.01.T01 AT5G20590 34.404 218 124 7 31 236 141 351 1.50e-29 118
MsG0580025833.01.T01 AT5G06230 32.222 270 154 12 15 265 46 305 9.66e-29 114
MsG0580025833.01.T01 AT3G62390 33.523 176 109 4 30 198 137 311 1.25e-27 113
MsG0580025833.01.T01 AT5G58600 30.041 243 147 8 28 263 63 289 2.89e-27 110
MsG0580025833.01.T01 AT2G40160 31.364 220 126 6 31 236 77 285 2.25e-26 107
MsG0580025833.01.T01 AT2G31110 31.674 221 136 6 30 245 44 254 1.43e-25 104
MsG0580025833.01.T01 AT2G30900 33.918 171 107 3 31 200 43 208 2.20e-25 104
MsG0580025833.01.T01 AT5G64470 28.214 280 159 13 31 286 55 316 3.64e-20 90.9
MsG0580025833.01.T01 AT1G78710 36.735 147 85 5 17 158 13 156 5.44e-20 89.7
MsG0580025833.01.T01 AT5G64470 28.938 273 152 13 31 279 55 309 6.26e-20 90.1
MsG0580025833.01.T01 AT2G34070 26.978 278 171 12 102 376 2 250 2.30e-16 78.6
MsG0580025833.01.T01 AT3G14850 25.091 275 172 10 102 371 2 247 1.41e-15 76.3

Find 78 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTTAATTACACTGAAAT+AGG 0.136015 5:-24864889 MsG0580025833.01.T01:CDS
TTCAAATATAGCTCATTATT+TGG 0.139372 5:+24865039 None:intergenic
TCCTATCAATTATGCTATTA+AGG 0.141791 5:+24864830 None:intergenic
ATGTATTAAGAAAAGCTTTA+AGG 0.157616 5:-24864858 MsG0580025833.01.T01:CDS
ATTGATTGTCCTTGCCATTT+TGG 0.188794 5:+24865154 None:intergenic
AGTATTGGATGTAACAAAAT+TGG 0.211311 5:-24864587 MsG0580025833.01.T01:CDS
TTTCTATAAGGCTTAGTCTT+TGG 0.240548 5:+24864718 None:intergenic
TTCTTCTCTCCATTTCTATA+AGG 0.257035 5:+24864706 None:intergenic
AAAGCCAAAGGCAATGAATT+TGG 0.260071 5:-24864625 MsG0580025833.01.T01:CDS
CTTAGTTGGAGAAAAGTAAT+TGG 0.278320 5:+24865279 None:intergenic
CCTAAACTTGTGTACCAAAA+TGG 0.283772 5:-24865168 MsG0580025833.01.T01:CDS
TGTAGTAATCACATTGGTTT+TGG 0.300724 5:+24865460 None:intergenic
TACAAAGAAGAGACTCTAAT+TGG 0.322908 5:+24865211 None:intergenic
CTTGGAAGTTTGCATTCATT+TGG 0.325913 5:+24865309 None:intergenic
CTAAAATTGTACCACCTTCA+TGG 0.327526 5:+24864926 None:intergenic
GTCTTAATTACACTGAAATA+GGG 0.334111 5:-24864888 MsG0580025833.01.T01:CDS
CTTTCCAAATTCATTGCCTT+TGG 0.341956 5:+24864621 None:intergenic
ATTCAACTATTTCCTTGTAT+TGG 0.358515 5:-24865104 MsG0580025833.01.T01:CDS
AATGGAAAAGGGTTCATATA+AGG 0.359801 5:+24864535 None:intergenic
TGTGTTTGTTCTTACTTAGT+TGG 0.369018 5:+24865265 None:intergenic
TGAATCATGTTGATGAGAAA+TGG 0.370491 5:-24865020 MsG0580025833.01.T01:CDS
TGTGTTCATTGGTGTTTGCC+TGG 0.372187 5:-24864478 MsG0580025833.01.T01:CDS
AAGACTAAGCCTTATAGAAA+TGG 0.384572 5:-24864715 MsG0580025833.01.T01:CDS
GACATGATTGTACTATCAAT+TGG 0.399358 5:-24864976 MsG0580025833.01.T01:CDS
CCTATCAATTATGCTATTAA+GGG 0.403903 5:+24864831 None:intergenic
ATAGTACAATCATGTCCATT+TGG 0.404533 5:+24864983 None:intergenic
ACCCCATTAAAGAATGGAAA+AGG 0.407132 5:+24864523 None:intergenic
GTGATTACTACAAGGGTGAT+TGG 0.411862 5:-24865449 MsG0580025833.01.T01:CDS
AAAGTTAAGATTTGAAGTAT+TGG 0.417492 5:-24864602 MsG0580025833.01.T01:CDS
TTGACTATCTTTATTGGAGA+TGG 0.423356 5:-24865335 MsG0580025833.01.T01:CDS
AAAGTAATTGGTTCAAACCT+TGG 0.424098 5:+24865291 None:intergenic
ATTGATAGGAGAGGAAATAA+AGG 0.428288 5:-24864817 MsG0580025833.01.T01:CDS
TGCAAAATGATTGTGTTCAT+TGG 0.437679 5:-24864489 MsG0580025833.01.T01:CDS
CAATAAAGATAGTCAAAATC+AGG 0.440358 5:+24865342 None:intergenic
ACACTTCATTCTTCACTCCT+AGG 0.443349 5:+24864397 None:intergenic
GAATCATGTTGATGAGAAAT+GGG 0.457430 5:-24865019 MsG0580025833.01.T01:CDS
CTTCCAGCTATTTACCATGA+AGG 0.459991 5:-24864940 MsG0580025833.01.T01:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5:+24864553 None:intergenic
AATGGAGAGAAGAAAGTTGA+AGG 0.470023 5:-24864697 MsG0580025833.01.T01:CDS
TTTAGAAATTATAAAGAAGT+GGG 0.470803 5:-24864428 MsG0580025833.01.T01:CDS
TGATTACTACAAGGGTGATT+GGG 0.476121 5:-24865448 MsG0580025833.01.T01:CDS
ATCTCATTCCATGTATCAAT+CGG 0.477028 5:+24864454 None:intergenic
CCACCTTCATGGTAAATAGC+TGG 0.482004 5:+24864937 None:intergenic
TTAAGACCAGATGGTCATCC+AGG 0.486939 5:-24864559 MsG0580025833.01.T01:CDS
TTGTTGGTGATTCTTTAGCT+AGG 0.490496 5:-24865236 MsG0580025833.01.T01:CDS
GGCAAAGGATATGAACCAAA+TGG 0.492253 5:-24864998 MsG0580025833.01.T01:CDS
AGAGAAGAAAGTTGAAGGAA+TGG 0.493088 5:-24864692 MsG0580025833.01.T01:CDS
ATGTTGTATCATTGTACAAA+GGG 0.502439 5:+24865409 None:intergenic
CCCTTAATAGCATAATTGAT+AGG 0.509822 5:-24864831 MsG0580025833.01.T01:CDS
CAAATGAATGCAAACTTCCA+AGG 0.514979 5:-24865308 MsG0580025833.01.T01:CDS
TCTTGAACCCCATTAAAGAA+TGG 0.524242 5:+24864517 None:intergenic
GAAGGTGCTTGGGATAAGGC+TGG 0.529140 5:-24864748 MsG0580025833.01.T01:CDS
TGCCTGGACCGATTGATACA+TGG 0.530575 5:-24864462 MsG0580025833.01.T01:CDS
GAAAATTGAGATAGAAGAAG+TGG 0.532759 5:-24864656 MsG0580025833.01.T01:CDS
TTGGCTTTGTTAAGACCAGA+TGG 0.544671 5:-24864568 MsG0580025833.01.T01:CDS
CCCCATTAAAGAATGGAAAA+GGG 0.551208 5:+24864524 None:intergenic
ACATGTGGCATGATGAAAGA+AGG 0.553278 5:-24865390 MsG0580025833.01.T01:CDS
AGAAATTATAAAGAAGTGGG+AGG 0.558622 5:-24864425 MsG0580025833.01.T01:CDS
CACCCTTGTAGTAATCACAT+TGG 0.566436 5:+24865454 None:intergenic
TTCCATGTATCAATCGGTCC+AGG 0.567285 5:+24864460 None:intergenic
GGAAGAAATTGCATGACACA+TGG 0.573080 5:-24865369 MsG0580025833.01.T01:CDS
AAACCAATGTGATTACTACA+AGG 0.577126 5:-24865457 MsG0580025833.01.T01:CDS
TTGTACTATCAATTGGACAT+TGG 0.581516 5:-24864969 MsG0580025833.01.T01:CDS
CATGTTGTATCATTGTACAA+AGG 0.603670 5:+24865408 None:intergenic
TGTTGATGAGAAATGGGCAA+AGG 0.615873 5:-24865013 MsG0580025833.01.T01:CDS
GAAGAAATTGCATGACACAT+GGG 0.618181 5:-24865368 MsG0580025833.01.T01:CDS
AAAGGGTTCATATAAGGACC+TGG 0.626122 5:+24864541 None:intergenic
AATAGCATAATTGATAGGAG+AGG 0.629684 5:-24864826 MsG0580025833.01.T01:CDS
AACCAATGTGATTACTACAA+GGG 0.630736 5:-24865456 MsG0580025833.01.T01:CDS
CCAGCTATTTACCATGAAGG+TGG 0.634066 5:-24864937 MsG0580025833.01.T01:CDS
AATTGAGATAGAAGAAGTGG+TGG 0.634610 5:-24864653 MsG0580025833.01.T01:CDS
GTGGTGGCTGCTAAAGCCAA+AGG 0.645087 5:-24864637 MsG0580025833.01.T01:CDS
AAGGAATGGATAGTGAGATG+AGG 0.645343 5:-24864678 MsG0580025833.01.T01:CDS
CAAGCACCTTCAAAATGATG+TGG 0.645352 5:+24864760 None:intergenic
TTGTACAATGATACAACATG+TGG 0.650483 5:-24865405 MsG0580025833.01.T01:CDS
ACTTGTGTACCAAAATGGCA+AGG 0.655571 5:-24865163 MsG0580025833.01.T01:CDS
AGAAGTGGGAGGAACAACCT+AGG 0.672972 5:-24864414 MsG0580025833.01.T01:CDS
GATTGGGTCAAAGACACTAG+AGG 0.682899 5:-24865432 MsG0580025833.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAGAAATTATAAAGAAGT+GGG - Chr5:24865514-24865533 MsG0580025833.01.T01:CDS 15.0%
!!! TTTTAGAAATTATAAAGAAG+TGG - Chr5:24865513-24865532 MsG0580025833.01.T01:CDS 15.0%
!! ATGTATTAAGAAAAGCTTTA+AGG - Chr5:24865084-24865103 MsG0580025833.01.T01:CDS 20.0%
!! TTCAAATATAGCTCATTATT+TGG + Chr5:24864906-24864925 None:intergenic 20.0%
!!! AAAGTTAAGATTTGAAGTAT+TGG - Chr5:24865340-24865359 MsG0580025833.01.T01:CDS 20.0%
! ATGTTGTATCATTGTACAAA+GGG + Chr5:24864536-24864555 None:intergenic 25.0%
! ATTCAACTATTTCCTTGTAT+TGG - Chr5:24864838-24864857 MsG0580025833.01.T01:CDS 25.0%
! CAATAAAGATAGTCAAAATC+AGG + Chr5:24864603-24864622 None:intergenic 25.0%
! CCTATCAATTATGCTATTAA+GGG + Chr5:24865114-24865133 None:intergenic 25.0%
! GTCTTAATTACACTGAAATA+GGG - Chr5:24865054-24865073 MsG0580025833.01.T01:CDS 25.0%
! TCAATACCTTGTACTAAAAA+TGG + Chr5:24864867-24864886 None:intergenic 25.0%
! TCCTATCAATTATGCTATTA+AGG + Chr5:24865115-24865134 None:intergenic 25.0%
!! AGTATTGGATGTAACAAAAT+TGG - Chr5:24865355-24865374 MsG0580025833.01.T01:CDS 25.0%
!!! CATATGATTGTTTGTTTTGT+TGG - Chr5:24864415-24864434 MsG0580025833.01.T01:CDS 25.0%
!!! CTGATTTTGACTATCTTTAT+TGG - Chr5:24864601-24864620 MsG0580025833.01.T01:CDS 25.0%
AAACCAATGTGATTACTACA+AGG - Chr5:24864485-24864504 MsG0580025833.01.T01:CDS 30.0%
AACCAATGTGATTACTACAA+GGG - Chr5:24864486-24864505 MsG0580025833.01.T01:CDS 30.0%
AATAGCATAATTGATAGGAG+AGG - Chr5:24865116-24865135 MsG0580025833.01.T01:CDS 30.0%
AATGGAAAAGGGTTCATATA+AGG + Chr5:24865410-24865429 None:intergenic 30.0%
AGAAATTATAAAGAAGTGGG+AGG - Chr5:24865517-24865536 MsG0580025833.01.T01:CDS 30.0%
ATAGTACAATCATGTCCATT+TGG + Chr5:24864962-24864981 None:intergenic 30.0%
ATCTCATTCCATGTATCAAT+CGG + Chr5:24865491-24865510 None:intergenic 30.0%
ATTGATAGGAGAGGAAATAA+AGG - Chr5:24865125-24865144 MsG0580025833.01.T01:CDS 30.0%
CATGTTGTATCATTGTACAA+AGG + Chr5:24864537-24864556 None:intergenic 30.0%
CCCTTAATAGCATAATTGAT+AGG - Chr5:24865111-24865130 MsG0580025833.01.T01:CDS 30.0%
CTTAGTTGGAGAAAAGTAAT+TGG + Chr5:24864666-24864685 None:intergenic 30.0%
GAAAATTGAGATAGAAGAAG+TGG - Chr5:24865286-24865305 MsG0580025833.01.T01:CDS 30.0%
GACATGATTGTACTATCAAT+TGG - Chr5:24864966-24864985 MsG0580025833.01.T01:CDS 30.0%
GGTCTTAATTACACTGAAAT+AGG - Chr5:24865053-24865072 MsG0580025833.01.T01:CDS 30.0%
TAAAAATGGAGACCAATACA+AGG + Chr5:24864853-24864872 None:intergenic 30.0%
TACAAAGAAGAGACTCTAAT+TGG + Chr5:24864734-24864753 None:intergenic 30.0%
TGCAAAATGATTGTGTTCAT+TGG - Chr5:24865453-24865472 MsG0580025833.01.T01:CDS 30.0%
TGTGTTTGTTCTTACTTAGT+TGG + Chr5:24864680-24864699 None:intergenic 30.0%
TTCTTCTCTCCATTTCTATA+AGG + Chr5:24865239-24865258 None:intergenic 30.0%
TTGACTATCTTTATTGGAGA+TGG - Chr5:24864607-24864626 MsG0580025833.01.T01:CDS 30.0%
TTGTACAATGATACAACATG+TGG - Chr5:24864537-24864556 MsG0580025833.01.T01:CDS 30.0%
TTGTACTATCAATTGGACAT+TGG - Chr5:24864973-24864992 MsG0580025833.01.T01:CDS 30.0%
! AAAGTAATTGGTTCAAACCT+TGG + Chr5:24864654-24864673 None:intergenic 30.0%
! AACCCTTTTCCATTCTTTAA+TGG - Chr5:24865416-24865435 MsG0580025833.01.T01:CDS 30.0%
! AAGACTAAGCCTTATAGAAA+TGG - Chr5:24865227-24865246 MsG0580025833.01.T01:CDS 30.0%
! ACCCTTTTCCATTCTTTAAT+GGG - Chr5:24865417-24865436 MsG0580025833.01.T01:CDS 30.0%
! ATGAAGGTGGTACAATTTTA+GGG - Chr5:24865018-24865037 MsG0580025833.01.T01:CDS 30.0%
! GAATCATGTTGATGAGAAAT+GGG - Chr5:24864923-24864942 MsG0580025833.01.T01:CDS 30.0%
! TGAATCATGTTGATGAGAAA+TGG - Chr5:24864922-24864941 MsG0580025833.01.T01:CDS 30.0%
! TTTACACCACATCATTTTGA+AGG - Chr5:24865176-24865195 MsG0580025833.01.T01:CDS 30.0%
! TTTCTATAAGGCTTAGTCTT+TGG + Chr5:24865227-24865246 None:intergenic 30.0%
!! AACAAACACATTGCTTTTGT+TGG - Chr5:24864690-24864709 MsG0580025833.01.T01:CDS 30.0%
!!! ATTTTAGGGTGTCATTATTG+TGG - Chr5:24865032-24865051 MsG0580025833.01.T01:CDS 30.0%
!!! TGTAGTAATCACATTGGTTT+TGG + Chr5:24864485-24864504 None:intergenic 30.0%
AAAGCCAAAGGCAATGAATT+TGG - Chr5:24865317-24865336 MsG0580025833.01.T01:CDS 35.0%
AATGGAGAGAAGAAAGTTGA+AGG - Chr5:24865245-24865264 MsG0580025833.01.T01:CDS 35.0%
AATTGAGATAGAAGAAGTGG+TGG - Chr5:24865289-24865308 MsG0580025833.01.T01:CDS 35.0%
ACCCCATTAAAGAATGGAAA+AGG + Chr5:24865422-24865441 None:intergenic 35.0%
AGAGAAGAAAGTTGAAGGAA+TGG - Chr5:24865250-24865269 MsG0580025833.01.T01:CDS 35.0%
CAAATGAATGCAAACTTCCA+AGG - Chr5:24864634-24864653 MsG0580025833.01.T01:CDS 35.0%
CCCCATTAAAGAATGGAAAA+GGG + Chr5:24865421-24865440 None:intergenic 35.0%
CCTAAACTTGTGTACCAAAA+TGG - Chr5:24864774-24864793 MsG0580025833.01.T01:CDS 35.0%
CTAAAATTGTACCACCTTCA+TGG + Chr5:24865019-24865038 None:intergenic 35.0%
CTTGGAAGTTTGCATTCATT+TGG + Chr5:24864636-24864655 None:intergenic 35.0%
CTTTCCAAATTCATTGCCTT+TGG + Chr5:24865324-24865343 None:intergenic 35.0%
GAAGAAATTGCATGACACAT+GGG - Chr5:24864574-24864593 MsG0580025833.01.T01:CDS 35.0%
TCTTGAACCCCATTAAAGAA+TGG + Chr5:24865428-24865447 None:intergenic 35.0%
TGATTACTACAAGGGTGATT+GGG - Chr5:24864494-24864513 MsG0580025833.01.T01:CDS 35.0%
! AGGACAATCAATTTTCTAGG+TGG - Chr5:24864799-24864818 MsG0580025833.01.T01:CDS 35.0%
! CATGAAGGTGGTACAATTTT+AGG - Chr5:24865017-24865036 MsG0580025833.01.T01:CDS 35.0%
! CCCTTTTCCATTCTTTAATG+GGG - Chr5:24865418-24865437 MsG0580025833.01.T01:CDS 35.0%
! GCAAGGACAATCAATTTTCT+AGG - Chr5:24864796-24864815 MsG0580025833.01.T01:CDS 35.0%
!! ATTGATTGTCCTTGCCATTT+TGG + Chr5:24864791-24864810 None:intergenic 35.0%
!! CCATTTTGGTACACAAGTTT+AGG + Chr5:24864777-24864796 None:intergenic 35.0%
!! TTGTTGGTGATTCTTTAGCT+AGG - Chr5:24864706-24864725 MsG0580025833.01.T01:CDS 35.0%
!!! ACATCATTTTGAAGGTGCTT+GGG - Chr5:24865184-24865203 MsG0580025833.01.T01:CDS 35.0%
!!! TGGTCTCCATTTTTAGTACA+AGG - Chr5:24864858-24864877 MsG0580025833.01.T01:CDS 35.0%
AAAGGGTTCATATAAGGACC+TGG + Chr5:24865404-24865423 None:intergenic 40.0%
AAGGAATGGATAGTGAGATG+AGG - Chr5:24865264-24865283 MsG0580025833.01.T01:CDS 40.0%
ACATGTGGCATGATGAAAGA+AGG - Chr5:24864552-24864571 MsG0580025833.01.T01:CDS 40.0%
ACTTGTGTACCAAAATGGCA+AGG - Chr5:24864779-24864798 MsG0580025833.01.T01:CDS 40.0%
CAAGCACCTTCAAAATGATG+TGG + Chr5:24865185-24865204 None:intergenic 40.0%
CACCCTTGTAGTAATCACAT+TGG + Chr5:24864491-24864510 None:intergenic 40.0%
CTTCCAGCTATTTACCATGA+AGG - Chr5:24865002-24865021 MsG0580025833.01.T01:CDS 40.0%
GGAAGAAATTGCATGACACA+TGG - Chr5:24864573-24864592 MsG0580025833.01.T01:CDS 40.0%
GGCAAAGGATATGAACCAAA+TGG - Chr5:24864944-24864963 MsG0580025833.01.T01:CDS 40.0%
GTGATTACTACAAGGGTGAT+TGG - Chr5:24864493-24864512 MsG0580025833.01.T01:CDS 40.0%
! TGTTGATGAGAAATGGGCAA+AGG - Chr5:24864929-24864948 MsG0580025833.01.T01:CDS 40.0%
!! TTGGCTTTGTTAAGACCAGA+TGG - Chr5:24865374-24865393 MsG0580025833.01.T01:CDS 40.0%
!!! CACATCATTTTGAAGGTGCT+TGG - Chr5:24865183-24865202 MsG0580025833.01.T01:CDS 40.0%
!!! TTTTGAAGGTGCTTGGGATA+AGG - Chr5:24865190-24865209 MsG0580025833.01.T01:CDS 40.0%
CCACCTTCATGGTAAATAGC+TGG + Chr5:24865008-24865027 None:intergenic 45.0%
CCAGCTATTTACCATGAAGG+TGG - Chr5:24865005-24865024 MsG0580025833.01.T01:CDS 45.0%
GATTGGGTCAAAGACACTAG+AGG - Chr5:24864510-24864529 MsG0580025833.01.T01:CDS 45.0%
TTAAGACCAGATGGTCATCC+AGG - Chr5:24865383-24865402 MsG0580025833.01.T01:CDS 45.0%
TTCCATGTATCAATCGGTCC+AGG + Chr5:24865485-24865504 None:intergenic 45.0%
! TGTGTTCATTGGTGTTTGCC+TGG - Chr5:24865464-24865483 MsG0580025833.01.T01:CDS 45.0%
AGAAGTGGGAGGAACAACCT+AGG - Chr5:24865528-24865547 MsG0580025833.01.T01:CDS 50.0%
TAAGGACCTGGATGACCATC+TGG + Chr5:24865392-24865411 None:intergenic 50.0%
TGCCTGGACCGATTGATACA+TGG - Chr5:24865480-24865499 MsG0580025833.01.T01:CDS 50.0%
GTGGTGGCTGCTAAAGCCAA+AGG - Chr5:24865305-24865324 MsG0580025833.01.T01:CDS 55.0%
!! GAAGGTGCTTGGGATAAGGC+TGG - Chr5:24865194-24865213 MsG0580025833.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 24864402 24865562 24864402 ID=MsG0580025833.01;Name=MsG0580025833.01
Chr5 mRNA 24864402 24865562 24864402 ID=MsG0580025833.01.T01;Parent=MsG0580025833.01;Name=MsG0580025833.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|386
Chr5 exon 24864402 24865562 24864402 ID=MsG0580025833.01.T01:exon:4004;Parent=MsG0580025833.01.T01
Chr5 CDS 24864402 24865562 24864402 ID=MsG0580025833.01.T01:cds;Parent=MsG0580025833.01.T01
Gene Sequence

>MsG0580025833.01.T01

ATGATTATTTACTCATATGATTGTTTGTTTTGTTGGTTTACAAGACTTGACTATTTTATTTCAGAAAAGAAAAAATCTTACCAAAACCAATGTGATTACTACAAGGGTGATTGGGTCAAAGACACTAGAGGCCCTTTGTACAATGATACAACATGTGGCATGATGAAAGAAGGAAGAAATTGCATGACACATGGGAGACCTGATTTTGACTATCTTTATTGGAGATGGAAACCAAATGAATGCAAACTTCCAAGGTTTGAACCAATTACTTTTCTCCAACTAAGTAAGAACAAACACATTGCTTTTGTTGGTGATTCTTTAGCTAGGAACCAATTAGAGTCTCTTCTTTGTATGTTAGCAACTACTTCAATTCCTAAACTTGTGTACCAAAATGGCAAGGACAATCAATTTTCTAGGTGGTACTTTTCTTCATACAATTCAACTATTTCCTTGTATTGGTCTCCATTTTTAGTACAAGGTATTGAAAAATCAAACACAAGTCCAAATAATGAGCTATATTTGAATCATGTTGATGAGAAATGGGCAAAGGATATGAACCAAATGGACATGATTGTACTATCAATTGGACATTGGTTTTTGCTTCCAGCTATTTACCATGAAGGTGGTACAATTTTAGGGTGTCATTATTGTGGTCTTAATTACACTGAAATAGGGTTTTATGATGTATTAAGAAAAGCTTTAAGGACTACCCTTAATAGCATAATTGATAGGAGAGGAAATAAAGGTAATGAAATTGATGTAATTGTTACAACATTTACACCACATCATTTTGAAGGTGCTTGGGATAAGGCTGGTGCTTGTCCAAAGACTAAGCCTTATAGAAATGGAGAGAAGAAAGTTGAAGGAATGGATAGTGAGATGAGGAAAATTGAGATAGAAGAAGTGGTGGCTGCTAAAGCCAAAGGCAATGAATTTGGAAAGTTAAGATTTGAAGTATTGGATGTAACAAAATTGGCTTTGTTAAGACCAGATGGTCATCCAGGTCCTTATATGAACCCTTTTCCATTCTTTAATGGGGTTCAAGAGTATGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGACCGATTGATACATGGAATGAGATATTTTTAGAAATTATAAAGAAGTGGGAGGAACAACCTAGGAGTGAAGAATGA
Protein sequence

>MsG0580025833.01.T01

MIIYSYDCLFCWFTRLDYFISEKKKSYQNQCDYYKGDWVKDTRGPLYNDTTCGMMKEGRNCMTHGRPDFDYLYWRWKPNECKLPRFEPITFLQLSKNKHIAFVGDSLARNQLESLLCMLATTSIPKLVYQNGKDNQFSRWYFSSYNSTISLYWSPFLVQGIEKSNTSPNNELYLNHVDEKWAKDMNQMDMIVLSIGHWFLLPAIYHEGGTILGCHYCGLNYTEIGFYDVLRKALRTTLNSIIDRRGNKGNEIDVIVTTFTPHHFEGAWDKAGACPKTKPYRNGEKKVEGMDSEMRKIEIEEVVAAKAKGNEFGKLRFEVLDVTKLALLRPDGHPGPYMNPFPFFNGVQEYVQNDCVHWCLPGPIDTWNEIFLEIIKKWEEQPRSEE*