AlfalfaGEDB | MsG0580025853.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025853.01

KEGG: -
GO: GO:0003676 GO:0008270 GO:0043531
PFAM: PF00931 PF14111 PF14392

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025853.01.T01	AES96229.2	72.265	905	159	10	1	843	1	875	0	1233

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025853.01.T01	Q7XA40	44.595	814	381	19	1	792	1	766	0	619

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025853.01.T01	G7K1H6	72.265	905	159	10	1	843	1	875	0.0	1233

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380012085.01	MsG0580025853.01	0.806444	8.202953e-50	4.453021e-47
MsG0380012091.01	MsG0580025853.01	0.806523	7.891969e-50	4.292832e-47
MsG0380012321.01	MsG0580025853.01	0.829167	5.988531e-55	6.080791e-52
MsG0380012324.01	MsG0580025853.01	0.823212	1.563784e-53	1.337887e-50
MsG0580025850.01	MsG0580025853.01	0.846781	1.750170e-59	3.046119e-56
MsG0580025853.01	MsG0580029880.01	0.805532	1.275780e-49	6.765643e-47
MsG0580025853.01	MsG0680033465.01	0.824962	6.072935e-54	5.461201e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025853.01.T01	MTR_5g034770	72.265	905	159	10	1	843	1	875	0.0	1233
MsG0580025853.01.T01	MTR_5g035480	68.475	885	189	13	1	823	1	857	0.0	1123
MsG0580025853.01.T01	MTR_5g035480	42.748	262	109	6	615	845	749	1000	1.78e-36	150
MsG0580025853.01.T01	MTR_5g037450	63.088	829	250	11	1	803	1	799	0.0	988
MsG0580025853.01.T01	MTR_5g037450	55.140	107	47	1	736	841	989	1095	2.16e-29	127
MsG0580025853.01.T01	MTR_5g035240	61.054	873	297	12	1	848	1	855	0.0	986
MsG0580025853.01.T01	MTR_5g035280	61.899	874	292	11	1	849	1	858	0.0	979
MsG0580025853.01.T01	MTR_5g035280	67.347	98	32	0	746	843	993	1090	3.67e-32	137
MsG0580025853.01.T01	MTR_3g026660	57.409	911	315	8	1	848	1	901	0.0	969
MsG0580025853.01.T01	MTR_3g026660	64.583	96	34	0	748	843	1039	1134	3.77e-29	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025853.01.T01	AT3G14470	33.193	714	428	12	28	706	36	735	6.35e-110	373
MsG0580025853.01.T01	AT3G14470	33.193	714	428	12	28	706	36	735	6.35e-110	373
MsG0580025853.01.T01	AT3G14460	32.108	816	467	26	31	794	36	816	3.43e-99	348
MsG0580025853.01.T01	AT3G50950	30.100	701	439	16	1	668	1	683	5.42e-73	262
MsG0580025853.01.T01	AT3G50950	30.100	701	439	16	1	668	1	683	5.42e-73	262
MsG0580025853.01.T01	AT3G07040	25.346	939	569	32	1	840	1	906	2.71e-62	231
MsG0580025853.01.T01	AT5G35450	29.602	679	419	17	1	651	1	648	2.67e-59	222
MsG0580025853.01.T01	AT5G35450	29.602	679	419	17	1	651	1	648	2.67e-59	222
MsG0580025853.01.T01	AT3G46530	28.788	528	338	13	1	507	1	511	9.37e-55	207
MsG0580025853.01.T01	AT5G43470	29.675	647	373	21	1	592	1	620	3.90e-53	202

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Find 436 sgRNAs with CRISPR-Local

Find 844 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCTCATCACGTATGT+CGG	0.594315	5:-25182429	None:intergenic
AAACCTTTCTCAAAACGCTT+AGG	0.419788	5:-25179204	None:intergenic
AAACTCCTCCAAACATAGCT+TGG	0.479994	5:-25182741	None:intergenic
AAAGGAGTGGCGCTATGTCA+AGG	0.585130	5:+25173759	MsG0580025853.01.T01:CDS
AAAGGGAAACAGGTGAGGAT+TGG	0.535535	5:+25175327	MsG0580025853.01.T01:CDS
AAAGTGGAGCATAGGAACGG+GGG	0.678028	5:+25182797	MsG0580025853.01.T01:three_prime_UTR
AAATCGGGGTTGAACCCATC+CGG	0.497504	5:-25181370	None:intergenic
AAATGAAGAGCATACAAGAG+AGG	0.635355	5:+25172990	MsG0580025853.01.T01:CDS
AACAATGGGGACCATATCTG+AGG	0.669044	5:+25179241	MsG0580025853.01.T01:CDS
AACACAGAGTTCCTGGACAT+TGG	0.541129	5:+25183123	MsG0580025853.01.T01:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAACTACCTTCTCTTA+AGG	+	Chr5:25174978-25174997	MsG0580025853.01.T01:intron	30.0%
AAATCGAATTGAACAACTTG+AGG	+	Chr5:25181126-25181145	MsG0580025853.01.T01:intron	30.0%
AAGGAGATACTTAATAAGTG+TGG	+	Chr5:25173679-25173698	MsG0580025853.01.T01:CDS	30.0%
AATATTATCAGCTATCGATG+AGG	-	Chr5:25176188-25176207	None:intergenic	30.0%
AATGAATAAGAACGGTGATA+AGG	-	Chr5:25181944-25181963	None:intergenic	30.0%
ACCTTTACCATATAAGAGTT+CGG	+	Chr5:25181307-25181326	MsG0580025853.01.T01:intron	30.0%
ACTTGGAGATATGAGAAAAA+TGG	+	Chr5:25183041-25183060	MsG0580025853.01.T01:three_prime_UTR	30.0%
AGAGAATGTTAAATGCTTTC+TGG	+	Chr5:25182484-25182503	MsG0580025853.01.T01:three_prime_UTR	30.0%
AGCAAAAATCAAAAGACAAC+AGG	-	Chr5:25180177-25180196	None:intergenic	30.0%
AGGAAATTCTTAATCTTTGG+AGG	-	Chr5:25183173-25183192	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	25172629	25183340	25172629	ID=MsG0580025853.01;Name=MsG0580025853.01
Chr5	mRNA	25172629	25183340	25172629	ID=MsG0580025853.01.T01;Parent=MsG0580025853.01;Name=MsG0580025853.01.T01;_AED=0.46;_eAED=0.63;_QI=0\|0\|0\|1\|1\|1\|7\|1788\|1353
Chr5	exon	25172629	25174863	25172629	ID=MsG0580025853.01.T01:exon:4041;Parent=MsG0580025853.01.T01
Chr5	exon	25175083	25175380	25175083	ID=MsG0580025853.01.T01:exon:4042;Parent=MsG0580025853.01.T01
Chr5	exon	25178827	25179999	25178827	ID=MsG0580025853.01.T01:exon:4043;Parent=MsG0580025853.01.T01
Chr5	exon	25180203	25180346	25180203	ID=MsG0580025853.01.T01:exon:4044;Parent=MsG0580025853.01.T01
Chr5	exon	25180435	25180644	25180435	ID=MsG0580025853.01.T01:exon:4045;Parent=MsG0580025853.01.T01
Chr5	exon	25181323	25181882	25181323	ID=MsG0580025853.01.T01:exon:4046;Parent=MsG0580025853.01.T01
Chr5	exon	25182111	25183340	25182111	ID=MsG0580025853.01.T01:exon:4047;Parent=MsG0580025853.01.T01
Chr5	CDS	25172629	25174863	25172629	ID=MsG0580025853.01.T01:cds;Parent=MsG0580025853.01.T01
Chr5	CDS	25175083	25175380	25175083	ID=MsG0580025853.01.T01:cds;Parent=MsG0580025853.01.T01
Chr5	CDS	25178827	25179999	25178827	ID=MsG0580025853.01.T01:cds;Parent=MsG0580025853.01.T01
Chr5	CDS	25180203	25180346	25180203	ID=MsG0580025853.01.T01:cds;Parent=MsG0580025853.01.T01
Chr5	CDS	25180435	25180644	25180435	ID=MsG0580025853.01.T01:cds;Parent=MsG0580025853.01.T01
Chr5	CDS	25181323	25181324	25181323	ID=MsG0580025853.01.T01:cds;Parent=MsG0580025853.01.T01
Chr5	three_prime_UTR	25181325	25181882	25181325	ID=MsG0580025853.01.T01:three_prime_utr;Parent=MsG0580025853.01.T01
Chr5	three_prime_UTR	25182111	25183340	25182111	ID=MsG0580025853.01.T01:three_prime_utr;Parent=MsG0580025853.01.T01

Gene Sequence

>MsG0580025853.01.T01

ATGGCTGAAGCTGTGCTTGAACTTGTCCTTGACAATTTAAGCTCACTCATTCAGAAGGAGATTGTCCTATTTCTTGGTTTCGAAAAAGACTTCAAAAGTCTTCAAAGCTTGATCACTGCAATCAAGGCTACTCTTGAAGATGCTGAGGAGAAACAATTCACTGACAAAGCTATCAAGGATTGGTTGCTTAAGCTTAAAGATGCTGCTTACGTCCTCGATGATATCTTGGACGAGTGTGCCACCAATGCACTGGAGTTAGAGTACAAAGGATCCAAGGGTGGACTACATGGTAAGCTACAAAGCTCTTGCTTATCCTCTCTTTATCCAAAACAAGTTGCTTTTCGTTACAAAATTGCTAAGAAAATGAAGAGCATACAAGAGAGGTTGGATCAAATAGATAAAGAAAAGAACATGTATCATTTCACTCAGATAGTAAGAGAGAAGAGAAGCGGAGTCCTTGACTGGCGTCAAACCACCTCAATCATTTCTCAACCTCAAGTCTATGGAAGAGATGAAGATAAGGATAAAATTGTAGATTTTTTGGTTGGCGAAGCTTCTGGTTTAAAGGATTTATCGGTCTATTCAATTGTGGGTCTAGGCGGACTTGGAAAAACAACACTTGCCCAACTTATCTTCAATCATGAAAGGGTGGTGCAGCATTTTGATCTAAGCATGTGGGTGTGTGTTTCAGATGATTTTAGTCTTAAAAGAATGACTAGAGCCATCATCGAACCTTGTGATGATTTGGATCTAGAGCTACTACAAAGAAGACTTCAGGATCTACTGCAAGGAAAAAGATTTTTGCTTGTATTGGATGACGTGTGGGACGATAAACAAGAGAATTGGCACAAGTTGAGATCTGTATTGGCTTGTGGCGGAAAGGGTGCTTCAATTTTGGTCACCACTCGTCTTGTGAAGGTAGCAGAAATCATGCGAACAATAACTCTGCATGATATATCAAAGCTATCCGACAAAGATTGTTGGGAATTGTTTAAAGAAAGAGCTTTTGCACCGAATGAAGTGGAACGTGAAGAGCTCGTGATCATAGGAAAGGAGATACTTAATAAGTGTGGAGGAGTGCCACTTGCAGCAATAGCACTAGGAAGTCTCTTGCGCTTTAAAAGAGAGGAAAAGGAGTGGCGCTATGTCAAGGAAAGCAAATTATGGAGTTTACATGATGAAGATAATGTCATGCCTGCGTTGAGATTAAGTTACTTGAATTTGTCGGTTAAACTGAGACAATGTTTTGCCTTCTGTGCGCTATTTCCCAAAGATGAAATAATAAGCAAGCAGTTCCTAATTGAGCTTTGGATGGCTAATGGCTTTATTTCATCCAATAAAATGTTGGGTGGGGAAGATATCGGTAATGAGGTGTGGAATGAACTATATTGGAGATCATTTTTTCAAGATTTTGAGAGAGATGTATTTGGGGAAATTATAAGTTTCAAGATGCACGATCTTGTTCATGATCTCGCTCGGTCAATTTCAGAAGAGGTGTGCTGCATTACAAAAAACGATGACATGCCGAATACATTAGAAAGAATTCGCTATCTCTCAGTTGAAAGCACAATTTCAATGCGTAACGTCAAATCAATGAGGACCTACACATCATATAACTTTACTACATCTTATTCTTCATCCAATGTATTCAATTTTCGTTCTTTACATGTGCTGAAGGTGATTTTATCTAATGTGTCATCTTCAATTGGTCATTTGAAGTGTTTAAGGTACTTGGATCTTTCTGAAGGTAAGTTTGAAACTCTTCCAAATTCCATATGTAAGTTGTGGAATTTACAAATTTTTAAATTAGACCGTTGTTTCAGACTCCGAAAATTGCCTAATAATTTGATACGCTTGAAATCTCTACAACATCTGTATTTGTTTGGCTGCTTCAGATTATCAAGTTTACCTCCCAATATAGGGAAGATGACTTCACTGAGGACTTTGAGCATGTATGTAGTTGGCAAGGAAAGTGGGTTCCTTTTGGAAGAAATAGGACAATTAAATTTCAAAGTAGATCAGCTTCACATCAAGCACCTTGAGAGAGTGAAAAGTGTAGAAGATGCTAAAGAAGCCAACATGTTGAGTAAGCACGTGACCGATTTGCAGTTGACGTGGGATGAAGAGTCCCAATTTCCGAGTCTATTCCTGAAGCAGAAGATTCTTAAGGAAAATGTGGAGCAGATTCTTGAAGTGCTCCAACCTTATCCCCAACAGCTTCAATATTTGTGGGTGGGATGCTCAAAAATAGAAGGTTTGGGTGAGGATTTACAACACATTACTTCCCTTCAATCGTTAACATCGGTGAATCTTCCAAACCTGACATCCTTACCAGACTCTTTAGGAAACCTTAGTTCGCTTCAAGGACTAGAAATTTATAAATGTCCGGAGTTGACTTGTCTTCCAGCGAGCATTCAAAGCCTTACTGGTCTGGAAAGTTTGAGTATTTCTGGTTGTCCTGAGTTGGAGAGACGGTGTAAAAGGGAAACAGGTGAGGATTGGCCAAAAATATCTCACATTCAAAATCTTGCAGGACCTTGGTTGTTTGATAGCTACCCTCTAATCTGGAGCAACGTTACTGATGGCAAAGATCCGTTCACTATGCCCATAGACTCAATTGAATTATGGGTGCAAGTCCATAATCTCCCCTTCAGTTTCATGACCGAGACTATGGGAATTCTGCTGGGCAATCATGTTGGAAAACTAGTCAAATATGATTATGAGAACAACTATGGGAACTGGAGAAGATACATGAGGCTGAGAGTATCTTTGCCGGTTAAAGAACCATTGAAGAAGAGTTTTGAATTTGTGTTGGAGGACGGTGCTGCTGTAAGGGTGAACTTCCGTTACGAGAAATCCGGAAATTTCTGCTATGAATGCGGTTTGATAGGCCATACAGATGGCTCCTGCCCTAAGCGTTTTGAGAAAGGTTTTGTGGAAGGACAACAACAATGGGGACCATATCTGAGGTCTGATTATATTGGACCTGAGGGAGGAATGATCGAGAATCCGTGGCTGCATGATGGTAGGAATAGGGGAAGGAGAACTTGGGCTCGTGCTGCTGAATGCTCTAACTTACATCGTGTGTTTGGTAGAGTTAGAATTGGAAGAGATGCGGTGACTAGAGGACTAGTCTTTTACAGACACATTGGTGCCCTATACGACTCTAACGAATGGGTGCGCTTTAATGTTGAAAGTGTTGGTCAGACACAGGATGCTGCTCAACCGGTTGGTTCCGAAACAATTCTGAACAGCAGTTTGCAGATTGCAGGTCCTGCAACACATGAAGTTCAGCTGGTAGAGGGAGAAGAGGCGCGAATTGAGCGACTGATACAAGAGGCCAAGCTTAAAAACCCGGTTATGATTGGGGATGCGATGAACAATACTGTGGAGTCTGAAAAGAATGTGAGTGAACAGCAGCCTATTTCTCAATTAGCTGCGTCCTTAGCCCGTATTCTGCATACAGGACTGCCTATAGGTGCTGAACCACGGGTGAACGGTAATGGCGTGATGCAGATTAACAAACCTGATGGTCAGAAGCAACTTAAGCGGCTAAGGTTGGAGGAAAGTGAAGAAAGTACTGCAGCAAGACAGAATGCAGAGACTGTCATTGATCCAAATAGGATAGATAGTAGTACTGTTATAGAAGTTTCTGATGTTGTAATGCATGAGTCTGTTTTGGCAGCAAATTCTAATGTAACGGCGGTTGATAGAATCGGTAGAGGTGGAGGGTTGGCTTTCTTATGGAAAAAATCAGTTAACTGCACTATCACAAACTACTCACAAAATCACATTGACATAGAAGTGATTGATTCCTTAATGGGAAAATGGCGTCTAACAGGTTTCTACGGTGATTTTAATGATATACTGTCAACCGAGGAAAAGAGAGGAAGGTCTGAAAGAGCGTCGTGGTTGATCCGTGGATTTCGACAAGCAGTGATAGACGTAGGATTAATTGATTTACATATGAGCGGGTATGCCTTCACTTGGTTTAAAAGCCTCGGCACTGTTAGAGCTGTAGAAGAGAAATTGGACAGAGCACTTATAAACTCAACTTGGTAG

Protein sequence

>MsG0580025853.01.T01

MAEAVLELVLDNLSSLIQKEIVLFLGFEKDFKSLQSLITAIKATLEDAEEKQFTDKAIKDWLLKLKDAAYVLDDILDECATNALELEYKGSKGGLHGKLQSSCLSSLYPKQVAFRYKIAKKMKSIQERLDQIDKEKNMYHFTQIVREKRSGVLDWRQTTSIISQPQVYGRDEDKDKIVDFLVGEASGLKDLSVYSIVGLGGLGKTTLAQLIFNHERVVQHFDLSMWVCVSDDFSLKRMTRAIIEPCDDLDLELLQRRLQDLLQGKRFLLVLDDVWDDKQENWHKLRSVLACGGKGASILVTTRLVKVAEIMRTITLHDISKLSDKDCWELFKERAFAPNEVEREELVIIGKEILNKCGGVPLAAIALGSLLRFKREEKEWRYVKESKLWSLHDEDNVMPALRLSYLNLSVKLRQCFAFCALFPKDEIISKQFLIELWMANGFISSNKMLGGEDIGNEVWNELYWRSFFQDFERDVFGEIISFKMHDLVHDLARSISEEVCCITKNDDMPNTLERIRYLSVESTISMRNVKSMRTYTSYNFTTSYSSSNVFNFRSLHVLKVILSNVSSSIGHLKCLRYLDLSEGKFETLPNSICKLWNLQIFKLDRCFRLRKLPNNLIRLKSLQHLYLFGCFRLSSLPPNIGKMTSLRTLSMYVVGKESGFLLEEIGQLNFKVDQLHIKHLERVKSVEDAKEANMLSKHVTDLQLTWDEESQFPSLFLKQKILKENVEQILEVLQPYPQQLQYLWVGCSKIEGLGEDLQHITSLQSLTSVNLPNLTSLPDSLGNLSSLQGLEIYKCPELTCLPASIQSLTGLESLSISGCPELERRCKRETGEDWPKISHIQNLAGPWLFDSYPLIWSNVTDGKDPFTMPIDSIELWVQVHNLPFSFMTETMGILLGNHVGKLVKYDYENNYGNWRRYMRLRVSLPVKEPLKKSFEFVLEDGAAVRVNFRYEKSGNFCYECGLIGHTDGSCPKRFEKGFVEGQQQWGPYLRSDYIGPEGGMIENPWLHDGRNRGRRTWARAAECSNLHRVFGRVRIGRDAVTRGLVFYRHIGALYDSNEWVRFNVESVGQTQDAAQPVGSETILNSSLQIAGPATHEVQLVEGEEARIERLIQEAKLKNPVMIGDAMNNTVESEKNVSEQQPISQLAASLARILHTGLPIGAEPRVNGNGVMQINKPDGQKQLKRLRLEESEESTAARQNAETVIDPNRIDSSTVIEVSDVVMHESVLAANSNVTAVDRIGRGGGLAFLWKKSVNCTITNYSQNHIDIEVIDSLMGKWRLTGFYGDFNDILSTEEKRGRSERASWLIRGFRQAVIDVGLIDLHMSGYAFTWFKSLGTVRAVEEKLDRALINSTW*