Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025890.01.T01 | GAU47022.1 | 80.328 | 61 | 12 | 0 | 4 | 64 | 89 | 149 | 2.18E-26 | 105 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025890.01.T01 | A0A2Z6PCK4 | 80.328 | 61 | 12 | 0 | 4 | 64 | 89 | 149 | 1.04e-26 | 105 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580025890.01 | MsG0680032157.01 | 0.803678 | 3.107858e-49 | 1.571408e-46 |
| MsG0380016475.01 | MsG0580025890.01 | 0.812717 | 3.683416e-51 | 2.359277e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025890.01.T01 | MTR_5g036470 | 80.328 | 61 | 12 | 0 | 4 | 64 | 277 | 337 | 2.69e-28 | 103 |
| MsG0580025890.01.T01 | MTR_5g036470 | 80.328 | 61 | 12 | 0 | 4 | 64 | 277 | 337 | 4.15e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025890.01.T01 | AT1G70150 | 71.429 | 56 | 16 | 0 | 9 | 64 | 286 | 341 | 1.99e-22 | 88.2 |
Find 17 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAACTGGACGCCCTCTTA+TGG | 0.278427 | 5:-26242463 | MsG0580025890.01.T01:CDS |
| AGTTGCATAACAACTCTAAC+TGG | 0.367883 | 5:-26242477 | MsG0580025890.01.T01:CDS |
| TTATTTAATGTTCTACCTAC+AGG | 0.368518 | 5:-26242776 | None:intergenic |
| TATATTATGCATTCCATAAG+AGG | 0.384281 | 5:+26242450 | None:intergenic |
| TACAATACTTGGCAACCAAC+TGG | 0.392018 | 5:+26242702 | None:intergenic |
| ACTTGGCAACCAACTGGAAT+AGG | 0.403575 | 5:+26242708 | None:intergenic |
| TAGGCAGCAATCCCAACATT+TGG | 0.421482 | 5:+26242727 | None:intergenic |
| TAGTGATTGCTCCAAATGTT+GGG | 0.441348 | 5:-26242738 | MsG0580025890.01.T01:CDS |
| GATGGAAGACGTACAATACT+TGG | 0.466699 | 5:+26242691 | None:intergenic |
| CTAGTGATTGCTCCAAATGT+TGG | 0.491358 | 5:-26242739 | MsG0580025890.01.T01:CDS |
| GGATTGCTGCCTATTCCAGT+TGG | 0.527394 | 5:-26242717 | MsG0580025890.01.T01:CDS |
| CAACATTTGGAGCAATCACT+AGG | 0.543169 | 5:+26242740 | None:intergenic |
| TATTTAATGTTCTACCTACA+GGG | 0.579202 | 5:-26242775 | None:intergenic |
| ATATTATGCATTCCATAAGA+GGG | 0.598303 | 5:+26242451 | None:intergenic |
| AGCAATCACTAGGTGAGGAA+AGG | 0.637028 | 5:+26242750 | None:intergenic |
| TTTGGAGCAATCACTAGGTG+AGG | 0.643639 | 5:+26242745 | None:intergenic |
| GGTGAGGAAAGGAACCCTGT+AGG | 0.687947 | 5:+26242761 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTTGATATGCAAACAA+AGG | + | Chr5:26242646-26242665 | None:intergenic | 20.0% |
| !! | TAAAACTAACTCATAAATAG+AGG | + | Chr5:26242563-26242582 | None:intergenic | 20.0% |
| ! | AATAATCAGTAAAAACAGCA+GGG | + | Chr5:26242695-26242714 | None:intergenic | 25.0% |
| ! | ATATTATGCATTCCATAAGA+GGG | + | Chr5:26242770-26242789 | None:intergenic | 25.0% |
| ! | TATATTATGCATTCCATAAG+AGG | + | Chr5:26242771-26242790 | None:intergenic | 25.0% |
| !! | TGTTTGCATATCAAATTTTC+AGG | - | Chr5:26242647-26242666 | MsG0580025890.01.T01:intron | 25.0% |
| !!! | GAGTTAGTTTTAGTTTTTTG+CGG | - | Chr5:26242571-26242590 | MsG0580025890.01.T01:intron | 25.0% |
| AATAGAGGATAACGAAAAGA+TGG | + | Chr5:26242548-26242567 | None:intergenic | 30.0% | |
| ATAATCAGTAAAAACAGCAG+GGG | + | Chr5:26242694-26242713 | None:intergenic | 30.0% | |
| ATGCAAACAAAGGAAACATA+AGG | + | Chr5:26242636-26242655 | None:intergenic | 30.0% | |
| CAATAATCAGTAAAAACAGC+AGG | + | Chr5:26242696-26242715 | None:intergenic | 30.0% | |
| AGTTGCATAACAACTCTAAC+TGG | - | Chr5:26242741-26242760 | MsG0580025890.01.T01:CDS | 35.0% | |
| CCTTCAGTTCAAAAACTATG+CGG | - | Chr5:26242594-26242613 | MsG0580025890.01.T01:intron | 35.0% | |
| TAGTGATTGCTCCAAATGTT+GGG | - | Chr5:26242480-26242499 | MsG0580025890.01.T01:CDS | 35.0% | |
| CTAGTGATTGCTCCAAATGT+TGG | - | Chr5:26242479-26242498 | MsG0580025890.01.T01:CDS | 40.0% | |
| GATGGAAGACGTACAATACT+TGG | + | Chr5:26242530-26242549 | None:intergenic | 40.0% | |
| TACAATACTTGGCAACCAAC+TGG | + | Chr5:26242519-26242538 | None:intergenic | 40.0% | |
| ! | CAACATTTGGAGCAATCACT+AGG | + | Chr5:26242481-26242500 | None:intergenic | 40.0% |
| ! | CCGCATAGTTTTTGAACTGA+AGG | + | Chr5:26242597-26242616 | None:intergenic | 40.0% |
| ACTTGGCAACCAACTGGAAT+AGG | + | Chr5:26242513-26242532 | None:intergenic | 45.0% | |
| AGCAATCACTAGGTGAGGAA+AGG | + | Chr5:26242471-26242490 | None:intergenic | 45.0% | |
| TAGGCAGCAATCCCAACATT+TGG | + | Chr5:26242494-26242513 | None:intergenic | 45.0% | |
| ! | TTTGGAGCAATCACTAGGTG+AGG | + | Chr5:26242476-26242495 | None:intergenic | 45.0% |
| GGATTGCTGCCTATTCCAGT+TGG | - | Chr5:26242501-26242520 | MsG0580025890.01.T01:CDS | 50.0% | |
| TCTAACTGGACGCCCTCTTA+TGG | - | Chr5:26242755-26242774 | MsG0580025890.01.T01:CDS | 50.0% | |
| GGTGAGGAAAGGAACCCTGT+AGG | + | Chr5:26242460-26242479 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 26242449 | 26242791 | 26242449 | ID=MsG0580025890.01;Name=MsG0580025890.01 |
| Chr5 | mRNA | 26242449 | 26242791 | 26242449 | ID=MsG0580025890.01.T01;Parent=MsG0580025890.01;Name=MsG0580025890.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|0|0.5|2|0|69 |
| Chr5 | exon | 26242705 | 26242791 | 26242705 | ID=MsG0580025890.01.T01:exon:5167;Parent=MsG0580025890.01.T01 |
| Chr5 | exon | 26242449 | 26242571 | 26242449 | ID=MsG0580025890.01.T01:exon:5166;Parent=MsG0580025890.01.T01 |
| Chr5 | CDS | 26242705 | 26242791 | 26242705 | ID=MsG0580025890.01.T01:cds;Parent=MsG0580025890.01.T01 |
| Chr5 | CDS | 26242449 | 26242571 | 26242449 | ID=MsG0580025890.01.T01:cds;Parent=MsG0580025890.01.T01 |
| Gene Sequence |
| Protein sequence |
