AlfalfaGEDB | MsG0580025896.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025896.01

KEGG: K13945
GO: -
PFAM: PF03195

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025896.01.T01	XP_013449868.1	90.667	225	19	2	1	224	1	224	3.42E-143	409

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025896.01.T01	Q9M2J7	79.661	118	23	1	8	124	11	128	2.50E-67	209

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025896.01.T01	A0A072U3H8	90.667	225	19	2	1	224	1	224	1.63e-143	409

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TFs/TRs:

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Gene ID	Type	Classification
MsG0580025896.01.T01	TF	LOB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000367.01	MsG0580025896.01	0.823046	1.710514e-53	1.456693e-50
MsG0180001614.01	MsG0580025896.01	-0.805376	1.375448e-49	7.265288e-47
MsG0180001656.01	MsG0580025896.01	-0.803810	2.917984e-49	1.480437e-46
MsG0180003484.01	MsG0580025896.01	0.809692	1.668718e-50	9.864175e-48
MsG0180004322.01	MsG0580025896.01	-0.801379	9.259814e-49	4.415512e-46
MsG0180005127.01	MsG0580025896.01	0.821040	4.988574e-53	4.014164e-50
MsG0180005146.01	MsG0580025896.01	0.813088	3.055524e-51	1.976331e-48
MsG0180005819.01	MsG0580025896.01	0.804362	2.239887e-49	1.152666e-46
MsG0180006010.01	MsG0580025896.01	0.820275	7.476654e-53	5.889229e-50
MsG0180006253.01	MsG0580025896.01	0.802687	4.984778e-49	2.457309e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025896.01.T01	MTR_7g096610	90.667	225	19	2	1	224	1	224	4.14e-147	409
MsG0580025896.01.T01	MTR_1g070205	68.063	191	52	4	8	195	12	196	3.18e-88	260
MsG0580025896.01.T01	MTR_7g096530	70.755	106	31	0	3	108	9	114	5.50e-53	170
MsG0580025896.01.T01	MTR_5g080470	67.308	104	34	0	2	105	1	104	7.01e-50	161
MsG0580025896.01.T01	MTR_8g036085	69.903	103	31	0	8	110	23	125	1.86e-49	161
MsG0580025896.01.T01	MTR_8g036105	60.656	122	45	2	5	123	15	136	1.60e-47	156
MsG0580025896.01.T01	MTR_1g070220	67.308	104	34	0	5	108	12	115	5.30e-47	154
MsG0580025896.01.T01	MTR_7g074990	68.317	101	32	0	8	108	20	120	1.05e-46	154
MsG0580025896.01.T01	MTR_7g075230	59.690	129	43	5	5	131	9	130	4.55e-44	146
MsG0580025896.01.T01	MTR_6g027710	56.000	125	53	1	7	129	39	163	6.37e-44	147

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025896.01.T01	AT3G58190	79.661	118	23	1	8	124	11	128	2.55e-68	209
MsG0580025896.01.T01	AT2G42440	74.046	131	33	1	2	131	1	131	4.28e-67	207
MsG0580025896.01.T01	AT2G31310	63.194	144	49	2	2	144	1	141	8.21e-61	189
MsG0580025896.01.T01	AT2G42430	70.093	107	32	0	4	110	11	117	2.18e-51	167
MsG0580025896.01.T01	AT4G00210	60.000	120	48	0	3	122	6	125	2.79e-48	158
MsG0580025896.01.T01	AT5G06080	65.421	107	37	0	2	108	1	107	2.29e-47	154
MsG0580025896.01.T01	AT3G03760	68.627	102	32	0	7	108	50	151	3.15e-47	157
MsG0580025896.01.T01	AT2G45420	71.000	100	29	0	11	110	40	139	8.26e-47	155
MsG0580025896.01.T01	AT4G00220	69.000	100	31	0	11	110	20	119	1.08e-44	149
MsG0580025896.01.T01	AT2G45410	60.870	115	43	1	11	123	19	133	3.19e-43	144

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Find 45 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCAACTTTGAAGATCTTG+AGG	0.528680	5:+26312253	None:intergenic
AAGAAATTGAGAACACATGC+TGG	0.541655	5:+26312216	None:intergenic
AAGAATTTGCAGGCTCCACA+AGG	0.586151	5:+26313115	None:intergenic
AATGGGTAGCACTTTGTTCA+TGG	0.389795	5:+26313047	None:intergenic
ACCAACTTTGAAGATCTTGA+GGG	0.534056	5:+26312254	None:intergenic
ACGGCCATGATGACTGGTTC+TGG	0.395558	5:-26313142	None:intergenic
ACTGGTTCTGGTTCTCCTTG+TGG	0.494488	5:-26313130	MsG0580025896.01.T01:CDS
AGAACCAGAACCAGTCATCA+TGG	0.567393	5:+26313138	None:intergenic
AGACTTCAAGATCCAATTTA+TGG	0.150762	5:-26312911	MsG0580025896.01.T01:CDS
ATGAACAAACCTGTTGTTGG+AGG	0.559046	5:+26312867	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGACTTCAAGATCCAATTTA+TGG	-	Chr5:26312231-26312250	MsG0580025896.01.T01:CDS	30.0%
ATTAGTGGAAGAAGAGAAAT+CGG	+	Chr5:26312953-26312972	None:intergenic	30.0%
CTTATGAATAGCTGCAAAAT+GGG	+	Chr5:26312115-26312134	None:intergenic	30.0%
TTGTTGGAGGGAAAAAATAT+GGG	+	Chr5:26312265-26312284	None:intergenic	30.0%
AAGAAATTGAGAACACATGC+TGG	+	Chr5:26312929-26312948	None:intergenic	35.0%
AATGTAAGCGTATACCATCT+AGG	+	Chr5:26312325-26312344	None:intergenic	35.0%
ATGAGATGCTTTCTTCCAAT+AGG	+	Chr5:26313062-26313081	None:intergenic	35.0%
ATGTAAGCGTATACCATCTA+GGG	+	Chr5:26312324-26312343	None:intergenic	35.0%
CCTTATGAATAGCTGCAAAA+TGG	+	Chr5:26312116-26312135	None:intergenic	35.0%
CTTAAACTGAAAACCCTAGA+TGG	-	Chr5:26312308-26312327	MsG0580025896.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	26312006	26313158	26312006	ID=MsG0580025896.01;Name=MsG0580025896.01
Chr5	mRNA	26312006	26313158	26312006	ID=MsG0580025896.01.T01;Parent=MsG0580025896.01;Name=MsG0580025896.01.T01;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|2\|0\|224
Chr5	exon	26312877	26313158	26312877	ID=MsG0580025896.01.T01:exon:5197;Parent=MsG0580025896.01.T01
Chr5	exon	26312006	26312398	26312006	ID=MsG0580025896.01.T01:exon:5196;Parent=MsG0580025896.01.T01
Chr5	CDS	26312877	26313158	26312877	ID=MsG0580025896.01.T01:cds;Parent=MsG0580025896.01.T01
Chr5	CDS	26312006	26312398	26312006	ID=MsG0580025896.01.T01:cds;Parent=MsG0580025896.01.T01

Gene Sequence

>MsG0580025896.01.T01

ATGATGACTGGTTCTGGTTCTCCTTGTGGAGCCTGCAAATTCTTGAGGAGGAAATGTGTTAAAGGTTGTGTTTTTGCACCTTACTTTTGCCATGAACAAAGTGCTACCCATTTTGCAGCTATTCATAAGGTCTTTGGTGCAAGTAATGTTTCAAAGCTCCTCGCACACCTCCCTGTGACTGATCGTTGTGAAGCTGCAGTCACAATCTCTTATGAAGCTCAAGCTAGACTTCAAGATCCAATTTATGGTTCTGTTGCCCATATTTTTTCCCTCCAACAACAGGTGGTCAATTTGCAAGCACAGCTAGCTTTTCTGAGAGAACAAGCAGCTCAAAGGTGTCTCAATAATGCCCCAAATTCTGAAAACCCTAATGAGAAGAATTTTGAAAAACCTACTAATATTCTCCCTCAAGATCTTCAAAGTTGGTTCCAGATGGAAAATTCCAGCATGTGTTCTCAATTTCTTCCCGATTTCTCTTCTTCCACTAATCTCTCATCAACAACACAATATTACAATGGGACTACAAACACTCTCATGGATCTAAACCCTATTGGAAACTATGAAAATTCAGGAACCCTATTGGAAGAAAGCATCTCATCATTTTCTACTTTTGATGAGCGACGTAATAATTCAATGTCCTATGACAACATGCAAAGACAGTGGACCTATCAGTAA

Protein sequence

>MsG0580025896.01.T01

MMTGSGSPCGACKFLRRKCVKGCVFAPYFCHEQSATHFAAIHKVFGASNVSKLLAHLPVTDRCEAAVTISYEAQARLQDPIYGSVAHIFSLQQQVVNLQAQLAFLREQAAQRCLNNAPNSENPNEKNFEKPTNILPQDLQSWFQMENSSMCSQFLPDFSSSTNLSSTTQYYNGTTNTLMDLNPIGNYENSGTLLEESISSFSTFDERRNNSMSYDNMQRQWTYQ*