Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026091.01.T01 | XP_003613691.1 | 95.522 | 134 | 6 | 0 | 1 | 134 | 1 | 134 | 2.66E-88 | 275 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026091.01.T01 | Q9C9L2 | 70.968 | 124 | 17 | 2 | 16 | 134 | 10 | 119 | 9.82E-50 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026091.01.T01 | G7KCJ2 | 95.522 | 134 | 6 | 0 | 1 | 134 | 1 | 134 | 1.27e-88 | 275 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0580026091.01.T01 | TF | TCP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580026091.01 | MsG0880045755.01 | 0.802526 | 5.380709e-49 | 2.641553e-46 |
| MsG0580026091.01 | MsG0880047251.01 | 0.811570 | 6.553104e-51 | 4.070553e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026091.01.T01 | MTR_5g039600 | 95.522 | 134 | 6 | 0 | 1 | 134 | 1 | 134 | 3.22e-92 | 275 |
| MsG0580026091.01.T01 | MTR_4g108370 | 66.667 | 138 | 27 | 2 | 16 | 134 | 27 | 164 | 1.94e-53 | 176 |
| MsG0580026091.01.T01 | MTR_2g006150 | 68.103 | 116 | 26 | 4 | 21 | 134 | 91 | 197 | 5.28e-43 | 149 |
| MsG0580026091.01.T01 | MTR_2g006150 | 68.103 | 116 | 26 | 4 | 21 | 134 | 91 | 197 | 8.78e-43 | 149 |
| MsG0580026091.01.T01 | MTR_7g028160 | 81.081 | 74 | 14 | 0 | 57 | 130 | 40 | 113 | 2.22e-39 | 135 |
| MsG0580026091.01.T01 | MTR_1g038650 | 79.221 | 77 | 11 | 1 | 58 | 134 | 28 | 99 | 1.92e-37 | 129 |
| MsG0580026091.01.T01 | MTR_8g033070 | 86.567 | 67 | 9 | 0 | 63 | 129 | 76 | 142 | 2.27e-37 | 131 |
| MsG0580026091.01.T01 | MTR_1g114380 | 71.910 | 89 | 16 | 2 | 50 | 134 | 17 | 100 | 3.72e-37 | 129 |
| MsG0580026091.01.T01 | MTR_1g063870 | 79.412 | 68 | 14 | 0 | 62 | 129 | 44 | 111 | 6.73e-35 | 122 |
| MsG0580026091.01.T01 | MTR_1g101810 | 82.812 | 64 | 11 | 0 | 66 | 129 | 63 | 126 | 1.44e-33 | 121 |
| MsG0580026091.01.T01 | MTR_1g101810 | 82.812 | 64 | 11 | 0 | 66 | 129 | 93 | 156 | 2.88e-33 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026091.01.T01 | AT1G69690 | 70.968 | 124 | 17 | 2 | 16 | 134 | 10 | 119 | 1.00e-50 | 166 |
| MsG0580026091.01.T01 | AT3G47620 | 54.878 | 164 | 32 | 3 | 7 | 134 | 27 | 184 | 1.39e-47 | 161 |
| MsG0580026091.01.T01 | AT1G58100 | 86.667 | 75 | 10 | 0 | 66 | 140 | 59 | 133 | 2.55e-40 | 140 |
| MsG0580026091.01.T01 | AT1G72010 | 92.647 | 68 | 5 | 0 | 67 | 134 | 63 | 130 | 6.19e-40 | 139 |
| MsG0580026091.01.T01 | AT2G45680 | 87.143 | 70 | 9 | 0 | 60 | 129 | 68 | 137 | 1.06e-38 | 135 |
| MsG0580026091.01.T01 | AT3G27010 | 80.000 | 75 | 15 | 0 | 54 | 128 | 65 | 139 | 1.01e-37 | 132 |
| MsG0580026091.01.T01 | AT3G27010 | 80.000 | 75 | 15 | 0 | 54 | 128 | 65 | 139 | 1.01e-37 | 132 |
| MsG0580026091.01.T01 | AT5G51910 | 76.923 | 78 | 18 | 0 | 57 | 134 | 47 | 124 | 1.89e-37 | 130 |
| MsG0580026091.01.T01 | AT5G51910 | 76.923 | 78 | 18 | 0 | 57 | 134 | 47 | 124 | 1.89e-37 | 130 |
| MsG0580026091.01.T01 | AT1G35560 | 88.235 | 68 | 8 | 0 | 67 | 134 | 55 | 122 | 1.16e-36 | 130 |
| MsG0580026091.01.T01 | AT5G08330 | 67.021 | 94 | 26 | 2 | 45 | 133 | 4 | 97 | 2.95e-36 | 126 |
| MsG0580026091.01.T01 | AT5G23280 | 77.632 | 76 | 12 | 1 | 58 | 133 | 35 | 105 | 4.81e-36 | 126 |
| MsG0580026091.01.T01 | AT2G37000 | 77.419 | 62 | 14 | 0 | 65 | 126 | 41 | 102 | 3.17e-29 | 107 |
| MsG0580026091.01.T01 | AT5G41030 | 58.974 | 78 | 26 | 1 | 51 | 128 | 58 | 129 | 2.17e-27 | 103 |
| MsG0580026091.01.T01 | AT3G45150 | 42.857 | 112 | 47 | 3 | 64 | 158 | 14 | 125 | 7.45e-23 | 89.7 |
| MsG0580026091.01.T01 | AT1G58100 | 55.844 | 77 | 10 | 1 | 66 | 142 | 59 | 111 | 1.65e-17 | 79.3 |
Find 58 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAAAGCTGAAGAGGGAAAT+TGG | 0.195439 | 5:+30596940 | None:intergenic |
| AGGCCGCCGGCATGCGGATC+CGG | 0.296288 | 5:+30596753 | None:intergenic |
| AGCAGTACAGCCATCATTTC+CGG | 0.303951 | 5:+30596862 | None:intergenic |
| GGTGCTCATCATCAAAATAA+TGG | 0.332278 | 5:-30596977 | MsG0580026091.01.T01:CDS |
| GTTCCAGCTCACGCGTGAGC+TGG | 0.358435 | 5:-30596717 | MsG0580026091.01.T01:CDS |
| GGCCGGAATTGTTCCTGTTC+CGG | 0.366988 | 5:+30596625 | None:intergenic |
| GTCCGTCAACCTTCGTGTGC+CGG | 0.377510 | 5:+30596783 | None:intergenic |
| GATTGAGTGGCTGCTTCAAC+AGG | 0.397983 | 5:-30596672 | MsG0580026091.01.T01:CDS |
| GTCATCGCCGCTACCGGAAC+AGG | 0.398111 | 5:-30596638 | MsG0580026091.01.T01:CDS |
| CCGGTAGCGGCGATGACGGC+TGG | 0.401247 | 5:+30596644 | None:intergenic |
| AGCTTAGAAACCGGGCTGAA+TGG | 0.413702 | 5:-30596488 | MsG0580026091.01.T01:CDS |
| CTGGAACACTCTGGCGGCAC+AGG | 0.419048 | 5:+30596733 | None:intergenic |
| TACCGGAACAGGAACAATTC+CGG | 0.434355 | 5:-30596627 | MsG0580026091.01.T01:CDS |
| AAAAGCTGAAGAGGGAAATT+GGG | 0.437875 | 5:+30596941 | None:intergenic |
| GAGGGAAATTGGGTCTGCTT+CGG | 0.440519 | 5:+30596951 | None:intergenic |
| GCTTAGAAACCGGGCTGAAT+GGG | 0.442453 | 5:-30596487 | MsG0580026091.01.T01:CDS |
| GTCCCAGCTCACGCGTGAGC+TGG | 0.449479 | 5:+30596714 | None:intergenic |
| CGACGGCTCAGCTTAGAAAC+CGG | 0.451524 | 5:-30596497 | MsG0580026091.01.T01:CDS |
| GTCGAAGCACGCTTCGGCGG+AGG | 0.452958 | 5:+30596812 | None:intergenic |
| AATATATCTCTTAGAAGCTC+AGG | 0.459346 | 5:-30596588 | MsG0580026091.01.T01:CDS |
| TTCCAGCTCACGCGTGAGCT+GGG | 0.468128 | 5:-30596716 | MsG0580026091.01.T01:CDS |
| AATTGTTCCTGTTCCGGTAG+CGG | 0.476759 | 5:+30596631 | None:intergenic |
| GGTTGAACAAGGTTGTTCTT+CGG | 0.480944 | 5:+30596886 | None:intergenic |
| ACTCTGGCGGCACAGGCCGC+CGG | 0.488107 | 5:+30596740 | None:intergenic |
| TCTTTCGTCGAAGCACGCTT+CGG | 0.491715 | 5:+30596806 | None:intergenic |
| CCAGCCGTCATCGCCGCTAC+CGG | 0.494047 | 5:-30596644 | MsG0580026091.01.T01:CDS |
| GTCGCGGAGGAGAACGAGCT+AGG | 0.495808 | 5:+30596516 | None:intergenic |
| TGTTCCGGTAGCGGCGATGA+CGG | 0.496727 | 5:+30596640 | None:intergenic |
| TGTTCAACCACCGGAAATGA+TGG | 0.498630 | 5:-30596872 | MsG0580026091.01.T01:CDS |
| GTTGTTCTTCGGAGACATCT+AGG | 0.503499 | 5:+30596897 | None:intergenic |
| CGGCACAGGCCGCCGGCATG+CGG | 0.505669 | 5:+30596747 | None:intergenic |
| AGGCCGCCGGATCCGCATGC+CGG | 0.515715 | 5:-30596759 | MsG0580026091.01.T01:CDS |
| CCGCCGGATCCGCATGCCGG+CGG | 0.521997 | 5:-30596756 | MsG0580026091.01.T01:CDS |
| GACCGGCACACGAAGGTTGA+CGG | 0.523997 | 5:-30596785 | MsG0580026091.01.T01:CDS |
| AGCGTGCTTCGACGAAAGAC+CGG | 0.538504 | 5:-30596802 | MsG0580026091.01.T01:CDS |
| ATATATTAGTGAAGTGAAGT+TGG | 0.545530 | 5:+30596604 | None:intergenic |
| TCATTTCCGGTGGTTGAACA+AGG | 0.546186 | 5:+30596875 | None:intergenic |
| AGCACGATCATGGAATTTGA+AGG | 0.546398 | 5:-30596998 | None:intergenic |
| CCGCCGGCATGCGGATCCGG+CGG | 0.547046 | 5:+30596756 | None:intergenic |
| CACACGAAGGTTGACGGACG+AGG | 0.549512 | 5:-30596779 | MsG0580026091.01.T01:CDS |
| ATTAGTGAAGTGAAGTTGGC+CGG | 0.566422 | 5:+30596608 | None:intergenic |
| TTCGTCGAAGCACGCTTCGG+CGG | 0.568824 | 5:+30596809 | None:intergenic |
| AGGTTGACGGACGAGGCCGC+CGG | 0.569788 | 5:-30596772 | MsG0580026091.01.T01:CDS |
| ACGCGTGAGCTGGAACACTC+TGG | 0.580832 | 5:+30596724 | None:intergenic |
| TAGTTCTGTCCCATTCAGCC+CGG | 0.583900 | 5:+30596478 | None:intergenic |
| GACGGCTCAGCTTAGAAACC+GGG | 0.596694 | 5:-30596496 | MsG0580026091.01.T01:CDS |
| GAGCTGGGACATAAATCAGA+TGG | 0.597190 | 5:-30596701 | MsG0580026091.01.T01:CDS |
| GAACAACCTTGTTCAACCAC+CGG | 0.600339 | 5:-30596881 | MsG0580026091.01.T01:CDS |
| ATAGTGAGACGGCGCAGACA+TGG | 0.611900 | 5:+30596560 | None:intergenic |
| TCGCGGAGGAGAACGAGCTA+GGG | 0.615115 | 5:+30596517 | None:intergenic |
| GTTTCTAAGCTGAGCCGTCG+CGG | 0.629887 | 5:+30596500 | None:intergenic |
| CAGATGGCGAGACGATTGAG+TGG | 0.645098 | 5:-30596685 | MsG0580026091.01.T01:CDS |
| AGTACAGCCATCATTTCCGG+TGG | 0.655208 | 5:+30596865 | None:intergenic |
| TAGCTCGTTCTCCTCCGCGA+CGG | 0.660506 | 5:-30596514 | MsG0580026091.01.T01:CDS |
| TCTAAGCTGAGCCGTCGCGG+AGG | 0.685215 | 5:+30596503 | None:intergenic |
| TTACCTCGAAAATAGTGAGA+CGG | 0.686470 | 5:+30596549 | None:intergenic |
| GACGAAAGACCGGCACACGA+AGG | 0.698145 | 5:-30596792 | MsG0580026091.01.T01:CDS |
| CGTGAGCTGGAACACTCTGG+CGG | 0.708891 | 5:+30596727 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATATTAGTGAAGTGAAGT+TGG | + | Chr5:30596863-30596882 | None:intergenic | 25.0% |
| AATATATCTCTTAGAAGCTC+AGG | - | Chr5:30596876-30596895 | MsG0580026091.01.T01:CDS | 30.0% | |
| !! | TTTTCTCTAAAAGCTGAAGA+GGG | + | Chr5:30596534-30596553 | None:intergenic | 30.0% |
| !! | TTTTTCTCTAAAAGCTGAAG+AGG | + | Chr5:30596535-30596554 | None:intergenic | 30.0% |
| AAAAGCTGAAGAGGGAAATT+GGG | + | Chr5:30596526-30596545 | None:intergenic | 35.0% | |
| GGTGCTCATCATCAAAATAA+TGG | - | Chr5:30596487-30596506 | MsG0580026091.01.T01:CDS | 35.0% | |
| TAAAAGCTGAAGAGGGAAAT+TGG | + | Chr5:30596527-30596546 | None:intergenic | 35.0% | |
| TTACCTCGAAAATAGTGAGA+CGG | + | Chr5:30596918-30596937 | None:intergenic | 35.0% | |
| ATTAGTGAAGTGAAGTTGGC+CGG | + | Chr5:30596859-30596878 | None:intergenic | 40.0% | |
| GGTTGAACAAGGTTGTTCTT+CGG | + | Chr5:30596581-30596600 | None:intergenic | 40.0% | |
| AATTGTTCCTGTTCCGGTAG+CGG | + | Chr5:30596836-30596855 | None:intergenic | 45.0% | |
| AGCAGTACAGCCATCATTTC+CGG | + | Chr5:30596605-30596624 | None:intergenic | 45.0% | |
| GAACAACCTTGTTCAACCAC+CGG | - | Chr5:30596583-30596602 | MsG0580026091.01.T01:CDS | 45.0% | |
| GAGCTGGGACATAAATCAGA+TGG | - | Chr5:30596763-30596782 | MsG0580026091.01.T01:CDS | 45.0% | |
| GTTGTTCTTCGGAGACATCT+AGG | + | Chr5:30596570-30596589 | None:intergenic | 45.0% | |
| TACCGGAACAGGAACAATTC+CGG | - | Chr5:30596837-30596856 | MsG0580026091.01.T01:CDS | 45.0% | |
| TCATTTCCGGTGGTTGAACA+AGG | + | Chr5:30596592-30596611 | None:intergenic | 45.0% | |
| TGTTCAACCACCGGAAATGA+TGG | - | Chr5:30596592-30596611 | MsG0580026091.01.T01:CDS | 45.0% | |
| AGCTTAGAAACCGGGCTGAA+TGG | - | Chr5:30596976-30596995 | MsG0580026091.01.T01:CDS | 50.0% | |
| AGTACAGCCATCATTTCCGG+TGG | + | Chr5:30596602-30596621 | None:intergenic | 50.0% | |
| GAGGGAAATTGGGTCTGCTT+CGG | + | Chr5:30596516-30596535 | None:intergenic | 50.0% | |
| GATTGAGTGGCTGCTTCAAC+AGG | - | Chr5:30596792-30596811 | MsG0580026091.01.T01:CDS | 50.0% | |
| GCTTAGAAACCGGGCTGAAT+GGG | - | Chr5:30596977-30596996 | MsG0580026091.01.T01:CDS | 50.0% | |
| TCTTTCGTCGAAGCACGCTT+CGG | + | Chr5:30596661-30596680 | None:intergenic | 50.0% | |
| ! | TAGTTCTGTCCCATTCAGCC+CGG | + | Chr5:30596989-30597008 | None:intergenic | 50.0% |
| AGCGTGCTTCGACGAAAGAC+CGG | - | Chr5:30596662-30596681 | MsG0580026091.01.T01:CDS | 55.0% | |
| ATAGTGAGACGGCGCAGACA+TGG | + | Chr5:30596907-30596926 | None:intergenic | 55.0% | |
| CAGATGGCGAGACGATTGAG+TGG | - | Chr5:30596779-30596798 | MsG0580026091.01.T01:CDS | 55.0% | |
| CGACGGCTCAGCTTAGAAAC+CGG | - | Chr5:30596967-30596986 | MsG0580026091.01.T01:CDS | 55.0% | |
| GACGGCTCAGCTTAGAAACC+GGG | - | Chr5:30596968-30596987 | MsG0580026091.01.T01:CDS | 55.0% | |
| GGCCGGAATTGTTCCTGTTC+CGG | + | Chr5:30596842-30596861 | None:intergenic | 55.0% | |
| GTTTCTAAGCTGAGCCGTCG+CGG | + | Chr5:30596967-30596986 | None:intergenic | 55.0% | |
| ! | GCGCCGTCTCACTATTTTCG+AGG | - | Chr5:30596912-30596931 | MsG0580026091.01.T01:CDS | 55.0% |
| ACGCGTGAGCTGGAACACTC+TGG | + | Chr5:30596743-30596762 | None:intergenic | 60.0% | |
| CGTGAGCTGGAACACTCTGG+CGG | + | Chr5:30596740-30596759 | None:intergenic | 60.0% | |
| GACCGGCACACGAAGGTTGA+CGG | - | Chr5:30596679-30596698 | MsG0580026091.01.T01:CDS | 60.0% | |
| GACGAAAGACCGGCACACGA+AGG | - | Chr5:30596672-30596691 | MsG0580026091.01.T01:CDS | 60.0% | |
| GTCCGTCAACCTTCGTGTGC+CGG | + | Chr5:30596684-30596703 | None:intergenic | 60.0% | |
| TAGCTCGTTCTCCTCCGCGA+CGG | - | Chr5:30596950-30596969 | MsG0580026091.01.T01:CDS | 60.0% | |
| TCGCGGAGGAGAACGAGCTA+GGG | + | Chr5:30596950-30596969 | None:intergenic | 60.0% | |
| TTCCAGCTCACGCGTGAGCT+GGG | - | Chr5:30596748-30596767 | MsG0580026091.01.T01:CDS | 60.0% | |
| TTCGTCGAAGCACGCTTCGG+CGG | + | Chr5:30596658-30596677 | None:intergenic | 60.0% | |
| ! | CACACGAAGGTTGACGGACG+AGG | - | Chr5:30596685-30596704 | MsG0580026091.01.T01:CDS | 60.0% |
| ! | TGTTCCGGTAGCGGCGATGA+CGG | + | Chr5:30596827-30596846 | None:intergenic | 60.0% |
| !! | TCGGCGGAGGCTTTTTCTGC+TGG | + | Chr5:30596642-30596661 | None:intergenic | 60.0% |
| CTGGAACACTCTGGCGGCAC+AGG | + | Chr5:30596734-30596753 | None:intergenic | 65.0% | |
| GTCATCGCCGCTACCGGAAC+AGG | - | Chr5:30596826-30596845 | MsG0580026091.01.T01:CDS | 65.0% | |
| GTCGCGGAGGAGAACGAGCT+AGG | + | Chr5:30596951-30596970 | None:intergenic | 65.0% | |
| GTTCCAGCTCACGCGTGAGC+TGG | - | Chr5:30596747-30596766 | MsG0580026091.01.T01:CDS | 65.0% | |
| TCTAAGCTGAGCCGTCGCGG+AGG | + | Chr5:30596964-30596983 | None:intergenic | 65.0% | |
| CCAGCCGTCATCGCCGCTAC+CGG | - | Chr5:30596820-30596839 | MsG0580026091.01.T01:CDS | 70.0% | |
| GTCCCAGCTCACGCGTGAGC+TGG | + | Chr5:30596753-30596772 | None:intergenic | 70.0% | |
| GTCGAAGCACGCTTCGGCGG+AGG | + | Chr5:30596655-30596674 | None:intergenic | 70.0% | |
| ! | AGGTTGACGGACGAGGCCGC+CGG | - | Chr5:30596692-30596711 | MsG0580026091.01.T01:CDS | 70.0% |
| ACTCTGGCGGCACAGGCCGC+CGG | + | Chr5:30596727-30596746 | None:intergenic | 75.0% | |
| AGGCCGCCGGATCCGCATGC+CGG | - | Chr5:30596705-30596724 | MsG0580026091.01.T01:CDS | 75.0% | |
| AGGCCGCCGGCATGCGGATC+CGG | + | Chr5:30596714-30596733 | None:intergenic | 75.0% | |
| ! | CCGGTAGCGGCGATGACGGC+TGG | + | Chr5:30596823-30596842 | None:intergenic | 75.0% |
| ! | CCGCCGGATCCGCATGCCGG+CGG | - | Chr5:30596708-30596727 | MsG0580026091.01.T01:CDS | 80.0% |
| ! | CCGCCGGCATGCGGATCCGG+CGG | + | Chr5:30596711-30596730 | None:intergenic | 80.0% |
| ! | CGGCACAGGCCGCCGGCATG+CGG | + | Chr5:30596720-30596739 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 30596475 | 30597011 | 30596475 | ID=MsG0580026091.01;Name=MsG0580026091.01 |
| Chr5 | mRNA | 30596475 | 30597011 | 30596475 | ID=MsG0580026091.01.T01;Parent=MsG0580026091.01;Name=MsG0580026091.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|178 |
| Chr5 | exon | 30596475 | 30597011 | 30596475 | ID=MsG0580026091.01.T01:exon:33859;Parent=MsG0580026091.01.T01 |
| Chr5 | CDS | 30596475 | 30597011 | 30596475 | ID=MsG0580026091.01.T01:cds;Parent=MsG0580026091.01.T01 |
| Gene Sequence |
| Protein sequence |
