AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045755.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045755.01.T01 MTR_8g078070 95.726 117 5 0 1 117 141 257 3.63e-76 230
MsG0880045755.01.T01 MTR_5g019460 81.034 116 22 0 2 117 141 256 1.07e-65 203
MsG0880045755.01.T01 MTR_2g072470 46.154 78 36 2 9 86 175 246 4.44e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045755.01.T01 AT4G36810 83.333 120 16 2 2 117 137 256 5.88e-66 204
MsG0880045755.01.T01 AT2G18620 82.353 119 18 2 1 117 115 232 2.89e-64 199
MsG0880045755.01.T01 AT2G23800 70.339 118 31 2 2 117 146 261 3.25e-52 169
MsG0880045755.01.T01 AT2G18640 69.748 119 32 2 1 117 141 257 2.26e-51 166
MsG0880045755.01.T01 AT3G14510 73.950 119 28 2 2 117 51 169 3.71e-47 153
MsG0880045755.01.T01 AT3G32040 73.950 119 28 2 2 117 127 245 2.55e-46 153
MsG0880045755.01.T01 AT3G29430 73.109 119 29 2 2 117 124 242 2.87e-45 150
MsG0880045755.01.T01 AT3G14530 70.588 119 32 2 2 117 127 245 2.57e-44 148
MsG0880045755.01.T01 AT3G14550 70.588 119 32 2 2 117 127 245 5.61e-44 147
MsG0880045755.01.T01 AT3G14550 70.588 119 32 2 2 117 127 245 5.61e-44 147
MsG0880045755.01.T01 AT3G14550 70.588 119 32 2 2 117 127 245 5.61e-44 147
MsG0880045755.01.T01 AT3G20160 59.167 120 43 3 2 117 112 229 5.85e-41 139
MsG0880045755.01.T01 AT1G49530 55.372 121 49 2 2 117 101 221 4.66e-40 136
MsG0880045755.01.T01 AT4G38460 51.261 119 50 3 2 118 109 221 5.56e-33 117
MsG0880045755.01.T01 AT3G20160 56.863 102 41 2 2 102 112 211 2.60e-32 115
MsG0880045755.01.T01 AT1G78510 43.590 78 38 2 9 86 121 192 9.12e-13 63.5
MsG0880045755.01.T01 AT1G78510 43.590 78 38 2 9 86 160 231 1.33e-12 63.2
MsG0880045755.01.T01 AT1G17050 42.308 78 39 2 9 86 171 242 3.18e-12 62.4

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCTTCTCCGAAGACTTTG+TGG 0.343711 8:-63828694 None:intergenic
GGCTCGGAAGGACTCGTTGC+TGG 0.376904 8:+63828836 MsG0880045755.01.T01:CDS
CCACGACGACCTTCCTTGTA+TGG 0.400090 8:+63828637 MsG0880045755.01.T01:CDS
ATTTCAACGGCGCAGGCGGC+CGG 0.436594 8:-63828593 None:intergenic
TTGCTAATTCACCGATTGAC+CGG 0.457857 8:-63828805 None:intergenic
GCGAGAATTGTCCGGTCAAT+CGG 0.462945 8:+63828794 MsG0880045755.01.T01:CDS
GGTGAATTAGCAAAATCAAT+TGG 0.470164 8:+63828815 MsG0880045755.01.T01:CDS
CGCCGGAGAAACACCGGCGG+TGG 0.473224 8:-63828774 None:intergenic
CTCCACCGCCGGTGTTTCTC+CGG 0.475973 8:+63828772 MsG0880045755.01.T01:CDS
ACTACAAACCACAAAGTCTT+CGG 0.480685 8:+63828686 MsG0880045755.01.T01:CDS
AAAGGCGAGAAGAGCGTCAC+CGG 0.489587 8:-63828729 None:intergenic
TTTCTCCGGCGAGAATTGTC+CGG 0.500552 8:+63828786 MsG0880045755.01.T01:CDS
GTATGGATAACGACGATCTC+CGG 0.512700 8:+63828654 MsG0880045755.01.T01:CDS
GTGGATCATTTCAACGGCGC+AGG 0.524802 8:-63828600 None:intergenic
TCAGAAGGTTTATCCGATGT+TGG 0.530197 8:+63828878 MsG0880045755.01.T01:CDS
GCAAAATCAATTGGCTCGGA+AGG 0.538303 8:+63828824 MsG0880045755.01.T01:CDS
GTTGTTGATATCAACTCAGA+AGG 0.546305 8:+63828863 MsG0880045755.01.T01:CDS
GATCATTTCAACGGCGCAGG+CGG 0.547898 8:-63828597 None:intergenic
CATATCGCTGTCTCCACCGC+CGG 0.579244 8:+63828761 MsG0880045755.01.T01:CDS
AATTCTCGCCGGAGAAACAC+CGG 0.583236 8:-63828780 None:intergenic
ATTAGCAAAATCAATTGGCT+CGG 0.586837 8:+63828820 MsG0880045755.01.T01:CDS
GAAGACGTTGCTGTTCTCGC+CGG 0.587256 8:+63828710 MsG0880045755.01.T01:CDS
CATCGTGTGGATCATTTCAA+CGG 0.597412 8:-63828606 None:intergenic
AGATCGTCGTTATCCATACA+AGG 0.598288 8:-63828650 None:intergenic
AGCGATATGTTCGAAAGCAA+AGG 0.621182 8:-63828747 None:intergenic
CGTCGTTATCCATACAAGGA+AGG 0.629409 8:-63828646 None:intergenic
CCATACAAGGAAGGTCGTCG+TGG 0.653424 8:-63828637 None:intergenic
AAGCTGTTCGAGTCCAACAT+CGG 0.661724 8:-63828891 None:intergenic
TCATTCATCTGCATAAACTG+CGG 0.670014 8:+63828918 MsG0880045755.01.T01:CDS
CGTGGATTAGAGACATCGTG+TGG 0.678617 8:-63828619 None:intergenic
ATTGACCGGACAATTCTCGC+CGG 0.693302 8:-63828791 None:intergenic
GATAACGACGATCTCCGGCG+AGG 0.695961 8:+63828659 MsG0880045755.01.T01:CDS
TCTCGCCGGAGAAACACCGG+CGG 0.762110 8:-63828777 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATTAGCAAAATCAATTGGCT+CGG + Chr8:63828820-63828839 MsG0880045755.01.T01:CDS 30.0%
GGTGAATTAGCAAAATCAAT+TGG + Chr8:63828815-63828834 MsG0880045755.01.T01:CDS 30.0%
ACTACAAACCACAAAGTCTT+CGG + Chr8:63828686-63828705 MsG0880045755.01.T01:CDS 35.0%
GTTGTTGATATCAACTCAGA+AGG + Chr8:63828863-63828882 MsG0880045755.01.T01:CDS 35.0%
TCATTCATCTGCATAAACTG+CGG + Chr8:63828918-63828937 MsG0880045755.01.T01:CDS 35.0%
AGATCGTCGTTATCCATACA+AGG - Chr8:63828653-63828672 None:intergenic 40.0%
AGCGATATGTTCGAAAGCAA+AGG - Chr8:63828750-63828769 None:intergenic 40.0%
CATCGTGTGGATCATTTCAA+CGG - Chr8:63828609-63828628 None:intergenic 40.0%
TCAGAAGGTTTATCCGATGT+TGG + Chr8:63828878-63828897 MsG0880045755.01.T01:CDS 40.0%
! TTGCTAATTCACCGATTGAC+CGG - Chr8:63828808-63828827 None:intergenic 40.0%
AAGCTGTTCGAGTCCAACAT+CGG - Chr8:63828894-63828913 None:intergenic 45.0%
CGTCGTTATCCATACAAGGA+AGG - Chr8:63828649-63828668 None:intergenic 45.0%
GTATGGATAACGACGATCTC+CGG + Chr8:63828654-63828673 MsG0880045755.01.T01:CDS 45.0%
!! GCAAAATCAATTGGCTCGGA+AGG + Chr8:63828824-63828843 MsG0880045755.01.T01:CDS 45.0%
!! GGTTTGTAGTTTTACCTCGC+CGG - Chr8:63828676-63828695 None:intergenic 45.0%
AATTCTCGCCGGAGAAACAC+CGG - Chr8:63828783-63828802 None:intergenic 50.0%
ATTGACCGGACAATTCTCGC+CGG - Chr8:63828794-63828813 None:intergenic 50.0%
CGTCTTCTCCGAAGACTTTG+TGG - Chr8:63828697-63828716 None:intergenic 50.0%
GCGAGAATTGTCCGGTCAAT+CGG + Chr8:63828794-63828813 MsG0880045755.01.T01:CDS 50.0%
TTTCTCCGGCGAGAATTGTC+CGG + Chr8:63828786-63828805 MsG0880045755.01.T01:CDS 50.0%
!! CGTGGATTAGAGACATCGTG+TGG - Chr8:63828622-63828641 None:intergenic 50.0%
CCACGACGACCTTCCTTGTA+TGG + Chr8:63828637-63828656 MsG0880045755.01.T01:CDS 55.0%
CCATACAAGGAAGGTCGTCG+TGG - Chr8:63828640-63828659 None:intergenic 55.0%
GATCATTTCAACGGCGCAGG+CGG - Chr8:63828600-63828619 None:intergenic 55.0%
GTGGATCATTTCAACGGCGC+AGG - Chr8:63828603-63828622 None:intergenic 55.0%
! GAAGACGTTGCTGTTCTCGC+CGG + Chr8:63828710-63828729 MsG0880045755.01.T01:CDS 55.0%
!! AAAGGCGAGAAGAGCGTCAC+CGG - Chr8:63828732-63828751 None:intergenic 55.0%
CATATCGCTGTCTCCACCGC+CGG + Chr8:63828761-63828780 MsG0880045755.01.T01:CDS 60.0%
GATAACGACGATCTCCGGCG+AGG + Chr8:63828659-63828678 MsG0880045755.01.T01:CDS 60.0%
ATTTCAACGGCGCAGGCGGC+CGG - Chr8:63828596-63828615 None:intergenic 65.0%
CTCCACCGCCGGTGTTTCTC+CGG + Chr8:63828772-63828791 MsG0880045755.01.T01:CDS 65.0%
GGCTCGGAAGGACTCGTTGC+TGG + Chr8:63828836-63828855 MsG0880045755.01.T01:CDS 65.0%
TCTCGCCGGAGAAACACCGG+CGG - Chr8:63828780-63828799 None:intergenic 65.0%
CGCCGGAGAAACACCGGCGG+TGG - Chr8:63828777-63828796 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 63828584 63828949 63828584 ID=MsG0880045755.01;Name=MsG0880045755.01
Chr8 mRNA 63828584 63828949 63828584 ID=MsG0880045755.01.T01;Parent=MsG0880045755.01;Name=MsG0880045755.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|121
Chr8 exon 63828584 63828949 63828584 ID=MsG0880045755.01.T01:exon:24266;Parent=MsG0880045755.01.T01
Chr8 CDS 63828584 63828949 63828584 ID=MsG0880045755.01.T01:cds;Parent=MsG0880045755.01.T01
Gene Sequence

>MsG0880045755.01.T01

ATGGCGATGCCGGCCGCCTGCGCCGTTGAAATGATCCACACGATGTCTCTAATCCACGACGACCTTCCTTGTATGGATAACGACGATCTCCGGCGAGGTAAAACTACAAACCACAAAGTCTTCGGAGAAGACGTTGCTGTTCTCGCCGGTGACGCTCTTCTCGCCTTTGCTTTCGAACATATCGCTGTCTCCACCGCCGGTGTTTCTCCGGCGAGAATTGTCCGGTCAATCGGTGAATTAGCAAAATCAATTGGCTCGGAAGGACTCGTTGCTGGACAAGTTGTTGATATCAACTCAGAAGGTTTATCCGATGTTGGACTCGAACAGCTTGAATTCATTCATCTGCATAAACTGCGGCTTTGTTAG

Protein sequence

>MsG0880045755.01.T01

MAMPAACAVEMIHTMSLIHDDLPCMDNDDLRRGKTTNHKVFGEDVAVLAGDALLAFAFEHIAVSTAGVSPARIVRSIGELAKSIGSEGLVAGQVVDINSEGLSDVGLEQLEFIHLHKLRLC*