AlfalfaGEDB | MsG0580027357.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027357.01

KEGG: K15115
GO: -
PFAM: PF00153

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027357.01.T01	XP_003614555.1	98.785	247	3	0	11	257	108	354	1.83E-179	507
MsG0580027357.01.T01	XP_003614555.1	32.759	174	110	5	38	210	37	204	4.35E-13	79.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027357.01.T01	Q8RWA5	62.948	251	78	4	15	251	114	363	1.95E-100	300

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027357.01.T01	G7JZG8	98.785	247	3	0	11	257	108	354	8.73e-180	507

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010900.01	MsG0580027357.01	0.826672	2.385320e-54	2.251981e-51
MsG0580024829.01	MsG0580027357.01	0.830176	3.403590e-55	3.558916e-52
MsG0580027357.01	MsG0780036183.01	0.808051	3.744420e-50	2.119214e-47
MsG0580027357.01	MsG0880044145.01	0.801377	9.265274e-49	4.417970e-46

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PPI

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Gene1	Gene2	Type
MsG0580027357.01	MsG0380015215.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027357.01.T01	MTR_5g055410	98.785	247	3	0	11	257	108	354	0.0	507
MsG0580027357.01.T01	MTR_5g055410	32.759	174	110	5	38	210	37	204	5.26e-17	79.7
MsG0580027357.01.T01	MTR_7g109135	65.238	210	66	2	14	223	16	218	1.05e-93	275
MsG0580027357.01.T01	MTR_7g109135	65.238	210	66	2	14	223	37	239	4.48e-93	274
MsG0580027357.01.T01	MTR_1g087840	70.556	180	50	1	36	215	134	310	1.13e-92	276
MsG0580027357.01.T01	MTR_1g087840	32.749	171	105	5	38	204	35	199	4.10e-16	76.6
MsG0580027357.01.T01	MTR_7g109135	65.238	210	66	2	14	223	115	317	5.89e-92	274
MsG0580027357.01.T01	MTR_7g109135	29.778	225	147	6	6	227	6	222	2.75e-18	82.8
MsG0580027357.01.T01	MTR_7g109135	61.905	210	66	3	14	223	115	310	4.05e-84	254
MsG0580027357.01.T01	MTR_7g109135	27.948	229	147	7	6	231	6	219	2.06e-13	68.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027357.01.T01	AT1G25380	62.948	251	78	4	15	251	114	363	1.99e-101	300
MsG0580027357.01.T01	AT1G25380	31.868	182	103	6	38	210	37	206	6.49e-16	76.6
MsG0580027357.01.T01	AT1G25380	62.948	251	78	4	15	251	114	363	1.99e-101	300
MsG0580027357.01.T01	AT1G25380	31.868	182	103	6	38	210	37	206	6.49e-16	76.6
MsG0580027357.01.T01	AT2G47490	66.010	203	66	1	14	216	109	308	6.05e-91	271
MsG0580027357.01.T01	AT2G47490	32.022	178	108	6	38	210	33	202	9.17e-18	81.6
MsG0580027357.01.T01	AT5G66380	40.000	195	107	3	21	208	109	300	1.29e-39	139
MsG0580027357.01.T01	AT5G66380	29.167	216	140	7	21	233	9	214	9.31e-17	78.6
MsG0580027357.01.T01	AT2G37890	33.810	210	119	6	4	209	140	333	3.06e-24	99.8
MsG0580027357.01.T01	AT4G01100	30.841	214	121	6	15	208	88	294	1.97e-23	97.1

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Find 55 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAGGTGTTTCATAAGGA+AGG	0.559222	5:-59001999	MsG0580027357.01.T01:CDS
AAGGCCTACGAGGACTGTAT+AGG	0.494032	5:-59002840	MsG0580027357.01.T01:intron
AATATCTACAAACCCATTGT+GGG	0.534468	5:-59003295	MsG0580027357.01.T01:CDS
AATGTCACTGGCTTCTGGTT+TGG	0.260255	5:+59001842	None:intergenic
ACAAGGATTACTCATGAGGA+AGG	0.586525	5:-59002859	MsG0580027357.01.T01:CDS
ACTCATGAGGAAGGCCTACG+AGG	0.694705	5:-59002850	MsG0580027357.01.T01:CDS
ACTGTCGACAAGCTAAATCC+TGG	0.492266	5:-59002167	MsG0580027357.01.T01:CDS
AGCTGGTCTTTGTTTCCGAG+AGG	0.606604	5:+59001762	None:intergenic
AGTAGTGGAGTTCAATATGC+CGG	0.586236	5:-59002038	MsG0580027357.01.T01:CDS
ATAGCTGCTGCTGGAGCTGG+GGG	0.581342	5:-59003327	MsG0580027357.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTGTACATGGCAAAAAA+AGG	-	Chr5:59002855-59002874	MsG0580027357.01.T01:CDS	30.0%
AATATCTACAAACCCATTGT+GGG	-	Chr5:59001847-59001866	MsG0580027357.01.T01:CDS	30.0%
AGTACAGAACTTATTCAATG+AGG	-	Chr5:59002928-59002947	MsG0580027357.01.T01:intron	30.0%
ATAATGAAACCTCTTTAGAC+TGG	-	Chr5:59002586-59002605	MsG0580027357.01.T01:intron	30.0%
ATCGTCATTAAAACAGAAAG+AGG	+	Chr5:59002104-59002123	None:intergenic	30.0%
GTCAGCGATATAAATAACAA+TGG	-	Chr5:59002530-59002549	MsG0580027357.01.T01:intron	30.0%
TATTATATTCCTTGATGCAG+TGG	-	Chr5:59002768-59002787	MsG0580027357.01.T01:intron	30.0%
TCTATTAAGAACAACTCCTT+CGG	-	Chr5:59003199-59003218	MsG0580027357.01.T01:intron	30.0%
ATCTCTTTCCCAATGAATAG+TGG	-	Chr5:59002385-59002404	MsG0580027357.01.T01:intron	35.0%
ATCTTGTTAGATTGAGATGG+TGG	+	Chr5:59003359-59003378	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	59001749	59003415	59001749	ID=MsG0580027357.01;Name=MsG0580027357.01
Chr5	mRNA	59001749	59003415	59001749	ID=MsG0580027357.01.T01;Parent=MsG0580027357.01;Name=MsG0580027357.01.T01;_AED=0.49;_eAED=0.51;_QI=0\|0\|0\|1\|1\|1\|4\|0\|257
Chr5	exon	59003275	59003415	59003275	ID=MsG0580027357.01.T01:exon:22052;Parent=MsG0580027357.01.T01
Chr5	exon	59002841	59002935	59002841	ID=MsG0580027357.01.T01:exon:22051;Parent=MsG0580027357.01.T01
Chr5	exon	59002288	59002376	59002288	ID=MsG0580027357.01.T01:exon:22050;Parent=MsG0580027357.01.T01
Chr5	exon	59001749	59002197	59001749	ID=MsG0580027357.01.T01:exon:22049;Parent=MsG0580027357.01.T01
Chr5	CDS	59003275	59003415	59003275	ID=MsG0580027357.01.T01:cds;Parent=MsG0580027357.01.T01
Chr5	CDS	59002841	59002935	59002841	ID=MsG0580027357.01.T01:cds;Parent=MsG0580027357.01.T01
Chr5	CDS	59002288	59002376	59002288	ID=MsG0580027357.01.T01:cds;Parent=MsG0580027357.01.T01
Chr5	CDS	59001749	59002197	59001749	ID=MsG0580027357.01.T01:cds;Parent=MsG0580027357.01.T01

Gene Sequence

>MsG0580027357.01.T01

ATGACGATATTTGTTGGTAATACTGTCGCAGAGGGTTGCAACGAACTCACAACTATTGGAAATATTATAGCTGCTGCTGGAGCTGGGGGTGCAACAGCAATATCTACAAACCCATTGTGGGTTGTTAAGACAAGACTTCAAACACAAGGCATGAGGCCTAATGTTGTTCCTTACAAGAGTGTTCTCTCCGCTTTGACAAGGATTACTCATGAGGAAGGCCTACGAGGACTGTATAGTGGTATTTTGCCTTCATTAGCTGGCGTAAGTCATGTAGCAATTCAATTCCCAGCATATGAAAAGATAAAGTTGTACATGGCAAAAAAAGATAACACAACTGTCGACAAGCTAAATCCTGGGAGTGTTGCAATCGCATCATCAATTTCAAAAGTAACTGCCTCTGTAATGACTTATCCACACGAGGTAATACGTTCCCGGCTGCAAGAGCAAGGCCAAGCGAAAAATAGTAGTGGAGTTCAATATGCCGGTGTTATTGACTGTACTAAGAAGGTGTTTCATAAGGAAGGCATTAAGGGATTTTATCGCGGTTGCGCTACTAATCTATTAAGAACAACTCCTTCGGCTGTTATTACATTCACCAGTTATGAGATGATACACAGGTTTTTGACGCGAACTATACCACAAAATGAGCCTAACAAACCCAAACCAGAAGCCAGTGACATTGATATGAAAAATGGTGATGATAGGGGGGAATCACCACCATCTCAATCTAACAAGATTCCTCTCGGAAACAAAGACCAGCTAACAAGACATTGA

Protein sequence

>MsG0580027357.01.T01

MTIFVGNTVAEGCNELTTIGNIIAAAGAGGATAISTNPLWVVKTRLQTQGMRPNVVPYKSVLSALTRITHEEGLRGLYSGILPSLAGVSHVAIQFPAYEKIKLYMAKKDNTTVDKLNPGSVAIASSISKVTASVMTYPHEVIRSRLQEQGQAKNSSGVQYAGVIDCTKKVFHKEGIKGFYRGCATNLLRTTPSAVITFTSYEMIHRFLTRTIPQNEPNKPKPEASDIDMKNGDDRGESPPSQSNKIPLGNKDQLTRH*