Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015215.01.T01 | XP_003600696.1 | 99.472 | 379 | 2 | 0 | 1 | 379 | 1 | 379 | 0 | 757 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048532.01 | MsG0380015215.01 | 0.827839 | 1.253659e-54 | 1.224421e-51 |
| MsG0080048809.01 | MsG0380015215.01 | 0.815580 | 8.594615e-52 | 5.948713e-49 |
| MsG0180000151.01 | MsG0380015215.01 | -0.809206 | 2.121733e-50 | 1.238099e-47 |
| MsG0180000280.01 | MsG0380015215.01 | 0.821739 | 3.440057e-53 | 2.822396e-50 |
| MsG0180000604.01 | MsG0380015215.01 | -0.812438 | 4.239194e-51 | 2.695578e-48 |
| MsG0180000854.01 | MsG0380015215.01 | -0.816843 | 4.485899e-52 | 3.214154e-49 |
| MsG0180001311.01 | MsG0380015215.01 | -0.828639 | 8.040514e-55 | 8.038640e-52 |
| MsG0180003357.01 | MsG0380015215.01 | -0.820082 | 8.278563e-53 | 6.486683e-50 |
| MsG0180003807.01 | MsG0380015215.01 | -0.807682 | 4.485962e-50 | 2.514460e-47 |
| MsG0180004385.01 | MsG0380015215.01 | -0.824000 | 1.023126e-53 | 8.949562e-51 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015215.01.T01 | MTR_3g065190 | 99.472 | 379 | 2 | 0 | 1 | 379 | 1 | 379 | 0.0 | 757 |
| MsG0380015215.01.T01 | MTR_4g085540 | 40.491 | 163 | 94 | 3 | 25 | 186 | 34 | 194 | 4.56e-32 | 127 |
| MsG0380015215.01.T01 | MTR_4g085540 | 32.787 | 183 | 115 | 5 | 18 | 195 | 115 | 294 | 3.17e-21 | 95.9 |
| MsG0380015215.01.T01 | MTR_4g085540 | 29.500 | 200 | 121 | 5 | 16 | 198 | 204 | 400 | 5.26e-21 | 95.1 |
| MsG0380015215.01.T01 | MTR_3g102040 | 39.535 | 172 | 101 | 3 | 25 | 195 | 24 | 193 | 1.59e-31 | 126 |
| MsG0380015215.01.T01 | MTR_3g102040 | 32.461 | 191 | 117 | 6 | 18 | 199 | 105 | 292 | 1.79e-18 | 87.4 |
| MsG0380015215.01.T01 | MTR_3g102040 | 32.275 | 189 | 108 | 5 | 27 | 198 | 205 | 390 | 7.22e-17 | 82.8 |
| MsG0380015215.01.T01 | MTR_1g019150 | 34.071 | 226 | 133 | 6 | 25 | 247 | 32 | 244 | 1.86e-31 | 126 |
| MsG0380015215.01.T01 | MTR_1g019150 | 30.500 | 200 | 126 | 6 | 18 | 207 | 113 | 309 | 1.34e-20 | 94.0 |
| MsG0380015215.01.T01 | MTR_1g019150 | 31.579 | 190 | 109 | 5 | 27 | 198 | 213 | 399 | 8.61e-19 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015215.01.T01 | AT2G18510 | 87.500 | 256 | 24 | 2 | 1 | 256 | 1 | 248 | 7.24e-162 | 458 |
| MsG0380015215.01.T01 | AT4G34110 | 43.353 | 173 | 95 | 3 | 15 | 186 | 26 | 196 | 2.42e-35 | 137 |
| MsG0380015215.01.T01 | AT4G34110 | 29.293 | 198 | 120 | 5 | 18 | 198 | 208 | 402 | 1.43e-18 | 87.8 |
| MsG0380015215.01.T01 | AT4G34110 | 28.962 | 183 | 122 | 5 | 18 | 195 | 117 | 296 | 4.21e-18 | 86.7 |
| MsG0380015215.01.T01 | AT1G49760 | 41.111 | 180 | 96 | 6 | 25 | 198 | 45 | 220 | 2.97e-34 | 134 |
| MsG0380015215.01.T01 | AT1G49760 | 31.313 | 198 | 116 | 5 | 18 | 198 | 217 | 411 | 3.54e-21 | 95.9 |
| MsG0380015215.01.T01 | AT1G49760 | 41.111 | 180 | 96 | 6 | 25 | 198 | 45 | 220 | 2.97e-34 | 134 |
| MsG0380015215.01.T01 | AT1G49760 | 31.313 | 198 | 116 | 5 | 18 | 198 | 217 | 411 | 3.54e-21 | 95.9 |
| MsG0380015215.01.T01 | AT1G54080 | 34.518 | 197 | 119 | 5 | 26 | 218 | 64 | 254 | 7.41e-34 | 130 |
| MsG0380015215.01.T01 | AT1G54080 | 26.500 | 200 | 105 | 5 | 28 | 191 | 151 | 344 | 4.28e-16 | 79.7 |
Find 166 sgRNAs with CRISPR-Local
Find 462 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAATTCGTGTAAATA+AGG | 0.327127 | 3:-66930974 | MsG0380015215.01.T01:intron |
| AAACAGTGTCGTTCGCAGGC+AGG | 0.509011 | 3:-66931577 | MsG0380015215.01.T01:five_prime_UTR |
| AACAGTGTCGTTCGCAGGCA+GGG | 0.495333 | 3:-66931576 | MsG0380015215.01.T01:five_prime_UTR |
| AAGGCTGGGGCTTGTGGAGG+TGG | 0.338842 | 3:+66926294 | None:intergenic |
| AAGGTATGCAGGGACCTCCA+AGG | 0.630862 | 3:-66926125 | MsG0380015215.01.T01:CDS |
| AATAATTCCTCAGAAATCTG+AGG | 0.595856 | 3:+66931340 | None:intergenic |
| AATCAGCATCTTCTTCGCTG+CGG | 0.690362 | 3:+66931113 | None:intergenic |
| AATCATGTTAAGTACTTTAA+TGG | 0.175680 | 3:+66931008 | None:intergenic |
| AATGACGACTCGAATTGCGC+CGG | 0.600813 | 3:-66931440 | MsG0380015215.01.T01:CDS |
| ACACCATTAGCGAAGGGGCG+AGG | 0.534859 | 3:+66926348 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAAAAGTGGCAAGAAAG+GGG | + | Chr3:66930747-66930766 | None:intergenic | 30.0% | |
| AAAATTTAACCCTCAATGGA+TGG | + | Chr3:66929873-66929892 | None:intergenic | 30.0% | |
| AAATAAATATTAGCGGTGAG+TGG | + | Chr3:66928939-66928958 | None:intergenic | 30.0% | |
| AAATATACAACAGAATGCAG+TGG | + | Chr3:66926544-66926563 | None:intergenic | 30.0% | |
| AACAAAATAGATGGATGGAT+AGG | - | Chr3:66930159-66930178 | MsG0380015215.01.T01:intron | 30.0% | |
| AATAAATATTAGCGGTGAGT+GGG | + | Chr3:66928938-66928957 | None:intergenic | 30.0% | |
| AATAATTCCTCAGAAATCTG+AGG | + | Chr3:66925981-66926000 | None:intergenic | 30.0% | |
| AATTATAGATGTGTTACCCA+AGG | + | Chr3:66929628-66929647 | None:intergenic | 30.0% | |
| ACTAATCAACATCAAGGTTA+TGG | - | Chr3:66926170-66926189 | MsG0380015215.01.T01:CDS | 30.0% | |
| ACTATCTAATCAGACTCTTT+CGG | - | Chr3:66930195-66930214 | MsG0380015215.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 66925735 | 66931605 | 66925735 | ID=MsG0380015215.01;Name=MsG0380015215.01 |
| Chr3 | mRNA | 66925735 | 66931605 | 66925735 | ID=MsG0380015215.01.T01;Parent=MsG0380015215.01;Name=MsG0380015215.01.T01;_AED=0.37;_eAED=0.37;_QI=144|1|1|1|1|1|8|231|379 |
| Chr3 | exon | 66925735 | 66926495 | 66925735 | ID=MsG0380015215.01.T01:exon:23;Parent=MsG0380015215.01.T01 |
| Chr3 | exon | 66926602 | 66926695 | 66926602 | ID=MsG0380015215.01.T01:exon:22;Parent=MsG0380015215.01.T01 |
| Chr3 | exon | 66927311 | 66927397 | 66927311 | ID=MsG0380015215.01.T01:exon:21;Parent=MsG0380015215.01.T01 |
| Chr3 | exon | 66929846 | 66929911 | 66929846 | ID=MsG0380015215.01.T01:exon:20;Parent=MsG0380015215.01.T01 |
| Chr3 | exon | 66930015 | 66930077 | 66930015 | ID=MsG0380015215.01.T01:exon:19;Parent=MsG0380015215.01.T01 |
| Chr3 | exon | 66930975 | 66931031 | 66930975 | ID=MsG0380015215.01.T01:exon:18;Parent=MsG0380015215.01.T01 |
| Chr3 | exon | 66931111 | 66931199 | 66931111 | ID=MsG0380015215.01.T01:exon:17;Parent=MsG0380015215.01.T01 |
| Chr3 | exon | 66931308 | 66931605 | 66931308 | ID=MsG0380015215.01.T01:exon:16;Parent=MsG0380015215.01.T01 |
| Chr3 | five_prime_UTR | 66931462 | 66931605 | 66931462 | ID=MsG0380015215.01.T01:five_prime_utr;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66931308 | 66931461 | 66931308 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66931111 | 66931199 | 66931111 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66930975 | 66931031 | 66930975 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66930015 | 66930077 | 66930015 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66929846 | 66929911 | 66929846 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66927311 | 66927397 | 66927311 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66926602 | 66926695 | 66926602 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | CDS | 66925966 | 66926495 | 66925966 | ID=MsG0380015215.01.T01:cds;Parent=MsG0380015215.01.T01 |
| Chr3 | three_prime_UTR | 66925735 | 66925965 | 66925735 | ID=MsG0380015215.01.T01:three_prime_utr;Parent=MsG0380015215.01.T01 |
| Gene Sequence |
>MsG0380015215.01.T01 ATGACGACTCGAATTGCGCCGGGTGTGGGTGCGAATCTGCTCGGGCAACATTCGGCGGAGAGGAACCAAGATGCCACTGCTTACGTCGGTAATCTCGACCCTCAGATTTCTGAGGAATTATTGTGGGAGCTTTTTGTTCAAGCTGGTCCCGTAGTTAATGTGTATGTTCCAAAGGATAGAGTCACTAATCAACATCAAGGTTATGGATTTGTTGAATTCCGCAGCGAAGAAGATGCTGATTATGCCATTAAAGTACTTAACATGATTAAACTTTATGGAAAACCAATTCGTGTAAATAAGGCATCCCAAGATAAAAAGAGCTTGGATGTCGGGGCAAACCTTTTCATTGGCAATCTCGATCCTGATGTAGATGAGAAGCTCTTGTATGATACTTTCAGTGCATTTGGAGTTATTGTTACTAATCCCAAGATCATGAGAGATCCTGACACGGGGAATTCCCGAGGCTTTGGCTTCATTAGCTACGATTCATTTGAGGCATCTGATTCAGCTATTGAGGCAATGAATGGACAGTATCTTTGCAATCGTCAAATTACAGTGTCGTATGCTTATAAGAAAGACACTAAAGGCGAAAGGCACGGTACTCCCGCAGAAAGAGTTTTGGCTGCAAGTAATCCGACGGCACAGAAGAGCAGGCCTCATACTTTATTTGCAAGTGGGCCACCATCACTTCCCAATGCTCCTCAGGCTAATGGTACCATTCCTGCTCCAGTGCCTCCTCGCCCCTTCGCTAATGGTGTCGCCCCCCCTCCTATTCATGTCATCCAAGCACCACCTCCACAAGCCCCAGCCTTTCAGCCTATGCAGATGCCACCACCTGGACAACAAGCATGGCATCAACAACAGCAAGGTCAACCAATGATGCAACAGGGAATGCCTCCTCCAATGCAACAGTTCAGGCCACCTCACTCGATGCAGATGCCGCCGCCACCACCTCCACAAGGTATGCAGGGACCTCCAAGGCCTCTTCCACCGCCATCAGTAATGGCAGGCCAGCAACAAGTTTGGCGACCACCGCCACCACCTCAGCAACAGGGTGGACATCCTATGGGATATCCTCAGAACTCAATGCCGCCACCGCCACCTCCCAATCACCATGGAATGCGACCACCTTCAGGTTAA |
| Protein sequence |
>MsG0380015215.01.T01 MTTRIAPGVGANLLGQHSAERNQDATAYVGNLDPQISEELLWELFVQAGPVVNVYVPKDRVTNQHQGYGFVEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDSAIEAMNGQYLCNRQITVSYAYKKDTKGERHGTPAERVLAASNPTAQKSRPHTLFASGPPSLPNAPQANGTIPAPVPPRPFANGVAPPPIHVIQAPPPQAPAFQPMQMPPPGQQAWHQQQQGQPMMQQGMPPPMQQFRPPHSMQMPPPPPPQGMQGPPRPLPPPSVMAGQQQVWRPPPPPQQQGGHPMGYPQNSMPPPPPPNHHGMRPPSG* |
