AlfalfaGEDB | MsG0580027447.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027447.01

KEGG: K11982
GO: -
PFAM: PF13639

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027447.01.T01	AES97702.1	86.982	338	41	2	1	336	1	337	1.72E-153	444

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027447.01.T01	O22197	47.458	59	31	0	209	267	176	234	6.30E-13	72

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027447.01.T01	G7K8T3	86.982	338	41	2	1	336	1	337	8.24e-154	444

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001486.01	MsG0580027447.01	0.810609	1.058452e-50	6.409443e-48
MsG0180005127.01	MsG0580027447.01	0.829839	4.112123e-55	4.258959e-52
MsG0180005146.01	MsG0580027447.01	0.800269	1.559759e-48	7.231715e-46
MsG0180006253.01	MsG0580027447.01	0.821412	4.093151e-53	3.327605e-50
MsG0280009398.01	MsG0580027447.01	0.816788	4.617242e-52	3.303168e-49
MsG0380016233.01	MsG0580027447.01	0.832912	7.211900e-56	8.175269e-53
MsG0380016458.01	MsG0580027447.01	0.831285	1.820215e-55	1.966219e-52
MsG0380017200.01	MsG0580027447.01	0.825964	3.517424e-54	3.254604e-51
MsG0580024994.01	MsG0580027447.01	0.839300	1.720901e-57	2.366425e-54
MsG0580025007.01	MsG0580027447.01	0.827574	1.451488e-54	1.406663e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027447.01.T01	MTR_5g058530	86.982	338	41	2	1	336	1	337	2.09e-157	444
MsG0580027447.01.T01	MTR_2g007340	43.421	76	42	1	208	283	187	261	9.61e-15	74.3
MsG0580027447.01.T01	MTR_5g088720	49.153	59	29	1	209	267	233	290	1.14e-14	74.7
MsG0580027447.01.T01	MTR_5g088720	49.153	59	29	1	209	267	187	244	1.15e-14	74.3
MsG0580027447.01.T01	MTR_5g088720	49.153	59	29	1	209	267	192	249	1.15e-14	74.3
MsG0580027447.01.T01	MTR_0067s0100	50.847	59	28	1	209	267	177	234	1.31e-14	73.6
MsG0580027447.01.T01	MTR_3g072630	49.153	59	29	1	209	267	208	265	1.07e-13	71.2
MsG0580027447.01.T01	MTR_1g051105	50.000	60	29	1	208	267	145	203	1.30e-13	70.1
MsG0580027447.01.T01	MTR_4g064570	36.792	106	62	3	166	267	121	225	1.42e-13	71.2
MsG0580027447.01.T01	MTR_3g072630	49.153	59	29	1	209	267	208	265	1.79e-13	70.9

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027447.01.T01	AT1G60360	50.847	59	29	209	267	210	268	3.22e-15	75.9
MsG0580027447.01.T01	AT2G40830	47.458	59	31	209	267	176	234	6.42e-14	72.0
MsG0580027447.01.T01	AT2G40830	47.458	59	31	209	267	176	234	6.42e-14	72.0
MsG0580027447.01.T01	AT2G40830	47.458	59	31	209	267	176	234	6.42e-14	72.0
MsG0580027447.01.T01	AT2G40830	47.458	59	31	209	267	176	234	6.42e-14	72.0
MsG0580027447.01.T01	AT3G56580	49.153	59	30	209	267	172	230	1.00e-13	71.2
MsG0580027447.01.T01	AT3G56580	49.153	59	30	209	267	172	230	1.00e-13	71.2
MsG0580027447.01.T01	AT3G56580	49.153	59	30	209	267	172	230	1.00e-13	71.2
MsG0580027447.01.T01	AT3G19950	44.068	59	33	209	267	202	260	2.39e-13	70.5
MsG0580027447.01.T01	AT3G19950	44.068	59	33	209	267	260	318	2.87e-13	70.5

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Find 93 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGGAAGTGGAGGAGG+TGG	0.549842	5:+61067263	None:intergenic
AAACTATTAGTTACTTGTGG+TGG	0.567054	5:+61066606	None:intergenic
AAAGCCAACAATCCCCAAGG+TGG	0.707176	5:-61067463	MsG0580027447.01.T01:CDS
AACTTGTATGTATGTTTGCA+GGG	0.516610	5:+61066765	None:intergenic
AAGCATAGCTTATACCACCT+TGG	0.528619	5:+61067449	None:intergenic
AAGCGATCCCATGGATCTTG+AGG	0.594977	5:+61066960	None:intergenic
AAGGCGGTGGTGGCGGTGGT+AGG	0.525691	5:+61067090	None:intergenic
AATCCGGAGAAGATTGAAAA+AGG	0.308182	5:+61066577	None:intergenic
AATTCTAGTGGTGATAATGT+TGG	0.629591	5:+61067053	None:intergenic
ACAGTTTGAAATTGTAGTGG+TGG	0.569682	5:+61067209	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTATTAGTTACTTGTGG+TGG	+	Chr5:61067373-61067392	None:intergenic	30.0%
AACTTGTATGTATGTTTGCA+GGG	+	Chr5:61067214-61067233	None:intergenic	30.0%
TGCAAACTATTAGTTACTTG+TGG	+	Chr5:61067376-61067395	None:intergenic	30.0%
AATCCGGAGAAGATTGAAAA+AGG	+	Chr5:61067402-61067421	None:intergenic	35.0%
ATTATCACCACTAGAATTCC+CGG	-	Chr5:61066928-61066947	MsG0580027447.01.T01:CDS	35.0%
CTATGCTTTATACCAACAAG+TGG	-	Chr5:61066542-61066561	MsG0580027447.01.T01:CDS	35.0%
GAACTTGTATGTATGTTTGC+AGG	+	Chr5:61067215-61067234	None:intergenic	35.0%
GATGGTGTAAAAATAGGAAG+TGG	+	Chr5:61066725-61066744	None:intergenic	35.0%
TCTTCTGTAACATGAGAATC+CGG	+	Chr5:61067418-61067437	None:intergenic	35.0%
TTAAACTCCTCCATGCAAAT+AGG	+	Chr5:61067166-61067185	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	61066494	61067504	61066494	ID=MsG0580027447.01;Name=MsG0580027447.01
Chr5	mRNA	61066494	61067504	61066494	ID=MsG0580027447.01.T01;Parent=MsG0580027447.01;Name=MsG0580027447.01.T01;_AED=0.36;_eAED=0.36;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|336
Chr5	exon	61066494	61067504	61066494	ID=MsG0580027447.01.T01:exon:13490;Parent=MsG0580027447.01.T01
Chr5	CDS	61066494	61067504	61066494	ID=MsG0580027447.01.T01:cds;Parent=MsG0580027447.01.T01

Gene Sequence

>MsG0580027447.01.T01

ATGTCAATGAACCATGGCCAAAGCCAACAATCCCCAAGGTGGTATAAGCTATGCTTTATACCAACAAGTGGTGACAGTTCAGATGATTCAGACACAGAATCTGTAATCATATCTCCGAGTACTGCAACTCAAACTAGTACATGGCAACAACCTTCTCCGCCACATCCATCACTATCACCGCGGAATCCGGATCCTGAAATCGACTGGCTTTGTCCACCACCACCTCCTCCACTTCCTATTTTTACACCATCACGACTATCAGTACGATCAGTACCACCACTACAATTTCAAACTGTAAACCCAGAACCTCGAATCAATCAGCGGTCTTCACAACCATCGCTGCTGTCATCACAACCACCAAATCCAGAACCTCGAATCAACTGGAGTTCTCTCCTACCACCGCCACCACCGCCTTTTTTCCCACCATCACCAACATTATCACCACTAGAATTCCCGGCTTCAAACTCAGAACCTCGTATTAACCTGCAGTATTCAATACCACCACCACCGCCGCCATTACCACCTCAAGATCCATGGGATCGCTTCATTAATACTTTCCCTTCTTTGACCACGCATGGCCGGCCTGATGACGATGATCATAGTCAGTTCCTTCGAAGTCACATGGTGAAAATCTCAAAGTTGCATTTAAAAGATGATCTCTCTCACTGTCCTATTTGCATGGAGGAGTTTAAACTTGGTGACCAAGCATGTCAGTTACCCTGCAAACATACATACAAGTTCGAATGCATTCTTCGTTGGCTTAACACCAGCAAAACCTGTCCTGTTTGCAGGCTTCAGTTAGATGGTTTTGAAGGACAGGGTATTTCTTATAGCATACTTGATGACGACGAGAGTTTGGACCTTGAACCTCAAATTCCACCACAAGTAACTAATAGTTTGCAAAACCTTTTTCAATCTTCTCCGGATTCTCATGTTACAGAAGATGATGCAGGTGAAAACTCTGATGAAGCTGAGTATGACAGTGCCTGTGATGAGTTAGGTTGGTATTAA

Protein sequence

>MsG0580027447.01.T01

MSMNHGQSQQSPRWYKLCFIPTSGDSSDDSDTESVIISPSTATQTSTWQQPSPPHPSLSPRNPDPEIDWLCPPPPPPLPIFTPSRLSVRSVPPLQFQTVNPEPRINQRSSQPSLLSSQPPNPEPRINWSSLLPPPPPPFFPPSPTLSPLEFPASNSEPRINLQYSIPPPPPPLPPQDPWDRFINTFPSLTTHGRPDDDDHSQFLRSHMVKISKLHLKDDLSHCPICMEEFKLGDQACQLPCKHTYKFECILRWLNTSKTCPVCRLQLDGFEGQGISYSILDDDESLDLEPQIPPQVTNSLQNLFQSSPDSHVTEDDAGENSDEAEYDSACDELGWY*