AlfalfaGEDB | MsG0280009398.01

M. sativa cultivar ZhongmuNo.1 / MsG0280009398.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009398.01.T02	RHN74325.1	98.81	84	1	0	1	84	87	170	2.60E-51	168
MsG0280009398.01.T01	RHN74325.1	98.347	121	2	0	1	121	50	170	1.11E-79	241

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009398.01.T01	A0A396JCZ6	98.347	121	2	0	1	121	50	170	5.28e-80	241
MsG0280009398.01.T02	A0A396JCZ6	98.810	84	1	0	1	84	87	170	1.24e-51	168

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000161.01	MsG0280009398.01	0.811673	6.222891e-51	3.875681e-48
MsG0180000556.01	MsG0280009398.01	0.827033	1.956345e-54	1.866383e-51
MsG0180005127.01	MsG0280009398.01	0.808518	2.978121e-50	1.706385e-47
MsG0180005353.01	MsG0280009398.01	0.827260	1.725774e-54	1.657573e-51
MsG0280007163.01	MsG0280009398.01	0.806020	1.007343e-49	5.409811e-47
MsG0280008226.01	MsG0280009398.01	0.802281	6.043806e-49	2.948804e-46
MsG0280009398.01	MsG0280010617.01	0.810965	8.867123e-51	5.421249e-48
MsG0280009398.01	MsG0380016233.01	0.850384	1.758278e-60	3.438074e-57
MsG0280009398.01	MsG0380017579.01	0.807972	3.892885e-50	2.198671e-47
MsG0280009398.01	MsG0480023542.01	0.801806	7.568498e-49	3.648222e-46
MsG0280009398.01	MsG0480023575.01	0.809520	1.817000e-50	1.069152e-47
MsG0280009398.01	MsG0580024466.01	0.825275	5.122899e-54	4.647916e-51
MsG0280009398.01	MsG0580025007.01	0.801106	1.053146e-48	4.987748e-46
MsG0280009398.01	MsG0580025023.01	0.802392	5.732994e-49	2.805162e-46
MsG0280009398.01	MsG0580027447.01	0.816788	4.617242e-52	3.303168e-49
MsG0280009398.01	MsG0580027517.01	0.845033	5.224748e-59	8.595237e-56
MsG0280009398.01	MsG0580028510.01	0.813839	2.088759e-51	1.378936e-48
MsG0280009398.01	MsG0580028916.01	0.853754	1.938458e-61	4.232577e-58
MsG0280009398.01	MsG0680031464.01	0.830241	3.280714e-55	3.437045e-52
MsG0280009398.01	MsG0780036944.01	0.823181	1.590376e-53	1.359467e-50
MsG0280009398.01	MsG0780038985.01	0.805572	1.251499e-49	6.643732e-47
MsG0280009398.01	MsG0780040081.01	0.810847	9.403095e-51	5.730454e-48
MsG0280009398.01	MsG0780040515.01	0.806945	6.427212e-50	3.534275e-47
MsG0280009398.01	MsG0780040977.01	0.809037	2.305935e-50	1.339449e-47
MsG0280009398.01	MsG0780040991.01	0.811285	7.557129e-51	4.658890e-48
MsG0280009398.01	MsG0880042500.01	-0.808287	3.335903e-50	1.899875e-47
MsG0280009398.01	MsG0880043134.01	0.802773	4.785343e-49	2.364266e-46
MsG0280009398.01	MsG0880046403.01	0.818427	1.971701e-52	1.475532e-49
MsG0280009398.01	MsG0880047697.01	0.809033	2.310660e-50	1.342035e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009398.01.T02	MTR_2g062220	98.810	84	1	0	1	84	38	121	6.52e-55	166
MsG0280009398.01.T02	MTR_5g084250	60.714	84	27	2	2	84	44	122	6.85e-20	77.4
MsG0280009398.01.T02	MTR_5g084240	70.000	50	14	1	36	84	73	122	1.64e-13	61.2
MsG0280009398.01.T02	MTR_4g075500	37.805	82	48	1	1	79	47	128	3.37e-12	57.8
MsG0280009398.01.T01	MTR_2g062220	98.347	121	2	0	1	121	1	121	4.93e-83	238
MsG0280009398.01.T01	MTR_5g084250	58.407	113	41	2	10	121	15	122	5.94e-32	109
MsG0280009398.01.T01	MTR_5g084240	58.407	113	41	3	10	121	15	122	1.66e-23	88.2
MsG0280009398.01.T01	MTR_4g075500	39.450	109	63	1	11	116	20	128	2.13e-20	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280009398.01.T02	AT2G31940	68.293	82	25	1	1	81	38	119	2.53e-23	86.3
MsG0280009398.01.T02	AT5G19875	53.571	84	34	1	1	84	45	123	2.35e-20	78.6
MsG0280009398.01.T01	AT2G31940	62.185	119	44	1	1	118	1	119	4.01e-39	127
MsG0280009398.01.T01	AT5G19875	51.852	108	47	1	14	121	21	123	9.27e-30	103

Find 35 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTCACTTTCTTTCCAACTA+TGG	0.283255	2:-51313845	MsG0280009398.01.T01:CDS
TAGAGCCGGTGGAACTCCTT+GGG	0.357174	2:-51313768	MsG0280009398.01.T01:CDS
AGAAACCAAGGAATATTAGA+AGG	0.362836	2:+51313960	None:intergenic
CTTACCCTTCTAATATTCCT+TGG	0.384516	2:-51313965	MsG0280009398.01.T01:CDS
ACCGATCATGGAAAGCATAA+TGG	0.386374	2:-51313918	MsG0280009398.01.T01:CDS
GGAAGAAACAAAGGTGTAAA+GGG	0.399974	2:+51313990	None:intergenic
AACGTTCTTGAAAGGAAGAT+TGG	0.410328	2:+51313702	None:intergenic
TAATATTCCTTGGTTTCTCC+TGG	0.412143	2:-51313955	MsG0280009398.01.T01:CDS
AGGAAGAAACAAAGGTGTAA+AGG	0.428577	2:+51313989	None:intergenic
TCCATTATGCTTTCCATGAT+CGG	0.434522	2:+51313917	None:intergenic
ATAGAGCCGGTGGAACTCCT+TGG	0.438956	2:-51313769	MsG0280009398.01.T01:CDS
GGTGGAACTCCTTGGGGTGT+TGG	0.445372	2:-51313761	MsG0280009398.01.T01:CDS
CGGTTCATAGTTTGAGTACC+AGG	0.446094	2:+51313937	None:intergenic
TTACTTCACTTGCTGAGTAA+AGG	0.451038	2:+51313674	None:intergenic
AAAACCTCCATCTCCATAGT+TGG	0.453390	2:+51313832	None:intergenic
GGAATATTAGAAGGGTAAGT+AGG	0.453723	2:+51313969	None:intergenic
CTCAAACTATGAACCGATCA+TGG	0.453973	2:-51313930	MsG0280009398.01.T01:CDS
TTTCTTTCCAACTATGGAGA+TGG	0.486381	2:-51313839	MsG0280009398.01.T01:CDS
CATAATGGATCAAGTGAAGA+TGG	0.494149	2:-51313903	MsG0280009398.01.T01:CDS
CTATGAAGTGACTCTCTCTC+TGG	0.504095	2:+51313788	None:intergenic
CTTTCCAACTATGGAGATGG+AGG	0.529513	2:-51313836	MsG0280009398.01.T01:CDS
CAAAGGTGTAAAGGGAGAGC+TGG	0.535259	2:+51313998	None:intergenic
AGAGAGTCACTTCATAGAGC+CGG	0.559146	2:-51313782	MsG0280009398.01.T01:CDS
GGGTAAGTAGGAAGAAACAA+AGG	0.562765	2:+51313981	None:intergenic
AGGAAACCAACGTTCTTGAA+AGG	0.563716	2:+51313694	None:intergenic
AATCTTCCTTTCAAGAACGT+TGG	0.576619	2:-51313700	MsG0280009398.01.T01:CDS
ACAACAAGAAGAAGCAGAAG+TGG	0.590949	2:+51313866	None:intergenic
AACACCCCAAGGAGTTCCAC+CGG	0.591898	2:+51313763	None:intergenic
AGTGACTCTCTCTCTGGCAA+AGG	0.607388	2:+51313794	None:intergenic
GAAACCAAGGAATATTAGAA+GGG	0.608468	2:+51313961	None:intergenic
TGGTTGAGAGTTATGAGTAG+TGG	0.617241	2:+51314018	None:intergenic
AGAAACAAACCAACACCCCA+AGG	0.617569	2:+51313752	None:intergenic
TTGAGTACCAGGAGAAACCA+AGG	0.644332	2:+51313948	None:intergenic
GAGTCACTTCATAGAGCCGG+TGG	0.645638	2:-51313779	MsG0280009398.01.T01:CDS
AGAGCCGGTGGAACTCCTTG+GGG	0.677208	2:-51313767	MsG0280009398.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGAAACCAAGGAATATTAGA+AGG	+	Chr2:51313744-51313763	None:intergenic	30.0%
	GAAACCAAGGAATATTAGAA+GGG	+	Chr2:51313743-51313762	None:intergenic	30.0%
!!!	TCTTGTTGTTCTTCTTTTCA+TGG	-	Chr2:51313969-51313988	MsG0280009398.01.T01:CDS	30.0%
	AACGTTCTTGAAAGGAAGAT+TGG	+	Chr2:51314002-51314021	None:intergenic	35.0%
	AATCTTCCTTTCAAGAACGT+TGG	-	Chr2:51314001-51314020	MsG0280009398.01.T01:CDS	35.0%
	CTTACCCTTCTAATATTCCT+TGG	-	Chr2:51313736-51313755	MsG0280009398.01.T01:CDS	35.0%
	GGAATATTAGAAGGGTAAGT+AGG	+	Chr2:51313735-51313754	None:intergenic	35.0%
	GTTCACTTTCTTTCCAACTA+TGG	-	Chr2:51313856-51313875	MsG0280009398.01.T01:CDS	35.0%
	TAATATTCCTTGGTTTCTCC+TGG	-	Chr2:51313746-51313765	MsG0280009398.01.T01:CDS	35.0%
	TCCATTATGCTTTCCATGAT+CGG	+	Chr2:51313787-51313806	None:intergenic	35.0%
	TTTCTTTCCAACTATGGAGA+TGG	-	Chr2:51313862-51313881	MsG0280009398.01.T01:CDS	35.0%
!	AGGAAGAAACAAAGGTGTAA+AGG	+	Chr2:51313715-51313734	None:intergenic	35.0%
!	GGAAGAAACAAAGGTGTAAA+GGG	+	Chr2:51313714-51313733	None:intergenic	35.0%
!!	CATAATGGATCAAGTGAAGA+TGG	-	Chr2:51313798-51313817	MsG0280009398.01.T01:CDS	35.0%
	AAAACCTCCATCTCCATAGT+TGG	+	Chr2:51313872-51313891	None:intergenic	40.0%
	ACAACAAGAAGAAGCAGAAG+TGG	+	Chr2:51313838-51313857	None:intergenic	40.0%
	AGGAAACCAACGTTCTTGAA+AGG	+	Chr2:51314010-51314029	None:intergenic	40.0%
	CTCAAACTATGAACCGATCA+TGG	-	Chr2:51313771-51313790	MsG0280009398.01.T01:CDS	40.0%
	GGGTAAGTAGGAAGAAACAA+AGG	+	Chr2:51313723-51313742	None:intergenic	40.0%
	TGGTTGAGAGTTATGAGTAG+TGG	+	Chr2:51313686-51313705	None:intergenic	40.0%
!	ACCGATCATGGAAAGCATAA+TGG	-	Chr2:51313783-51313802	MsG0280009398.01.T01:CDS	40.0%
	AGAAACAAACCAACACCCCA+AGG	+	Chr2:51313952-51313971	None:intergenic	45.0%
	AGAGAGTCACTTCATAGAGC+CGG	-	Chr2:51313919-51313938	MsG0280009398.01.T01:CDS	45.0%
	CGGTTCATAGTTTGAGTACC+AGG	+	Chr2:51313767-51313786	None:intergenic	45.0%
	CTATGAAGTGACTCTCTCTC+TGG	+	Chr2:51313916-51313935	None:intergenic	45.0%
	CTTTCCAACTATGGAGATGG+AGG	-	Chr2:51313865-51313884	MsG0280009398.01.T01:CDS	45.0%
	TTGAGTACCAGGAGAAACCA+AGG	+	Chr2:51313756-51313775	None:intergenic	45.0%
	AGTGACTCTCTCTCTGGCAA+AGG	+	Chr2:51313910-51313929	None:intergenic	50.0%
!	CAAAGGTGTAAAGGGAGAGC+TGG	+	Chr2:51313706-51313725	None:intergenic	50.0%
	AACACCCCAAGGAGTTCCAC+CGG	+	Chr2:51313941-51313960	None:intergenic	55.0%
	ATAGAGCCGGTGGAACTCCT+TGG	-	Chr2:51313932-51313951	MsG0280009398.01.T01:CDS	55.0%
	GAGTCACTTCATAGAGCCGG+TGG	-	Chr2:51313922-51313941	MsG0280009398.01.T01:CDS	55.0%
	TAGAGCCGGTGGAACTCCTT+GGG	-	Chr2:51313933-51313952	MsG0280009398.01.T01:CDS	55.0%
	AGAGCCGGTGGAACTCCTTG+GGG	-	Chr2:51313934-51313953	MsG0280009398.01.T01:CDS	60.0%
	GGTGGAACTCCTTGGGGTGT+TGG	-	Chr2:51313940-51313959	MsG0280009398.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	51313679	51314044	51313679	ID=MsG0280009398.01;Name=MsG0280009398.01
Chr2	mRNA	51313679	51314044	51313679	ID=MsG0280009398.01.T01;Parent=MsG0280009398.01;Name=MsG0280009398.01.T01;_AED=0.32;_eAED=0.32;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|121
Chr2	exon	51313679	51314044	51313679	ID=MsG0280009398.01.T01:exon:44669;Parent=MsG0280009398.01.T01
Chr2	CDS	51313679	51314044	51313679	ID=MsG0280009398.01.T01:cds;Parent=MsG0280009398.01.T01
Chr2	mRNA	51313679	51313933	51313679	ID=MsG0280009398.01.T02;Parent=MsG0280009398.01;Name=MsG0280009398.01.T02;_AED=0.38;_eAED=0.38;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|84
Chr2	exon	51313679	51313933	51313679	ID=MsG0280009398.01.T02:exon:44670;Parent=MsG0280009398.01.T02
Chr2	CDS	51313679	51313933	51313679	ID=MsG0280009398.01.T02:cds;Parent=MsG0280009398.01.T02

Gene Sequence

>MsG0280009398.01.T02

ATGGAAAGCATAATGGATCAAGTGAAGATGGTTCTAATGATTTCACCACTTCTGCTTCTTCTTGTTGTTCACTTTCTTTCCAACTATGGAGATGGAGGTTTTTTTTCTTCTTTGATTCCTTTGCCAGAGAGAGAGTCACTTCATAGAGCCGGTGGAACTCCTTGGGGTGTTGGTTTGTTTCTTGTTGTTCTTCTTTTCATGGTTTCTTACCAATCTTCCTTTCAAGAACGTTGGTTTCCTTTACTCAGCAAGTGA

>MsG0280009398.01.T01

ATGGCCACTACTCATAACTCTCAACCAGCTCTCCCTTTACACCTTTGTTTCTTCCTACTTACCCTTCTAATATTCCTTGGTTTCTCCTGGTACTCAAACTATGAACCGATCATGGAAAGCATAATGGATCAAGTGAAGATGGTTCTAATGATTTCACCACTTCTGCTTCTTCTTGTTGTTCACTTTCTTTCCAACTATGGAGATGGAGGTTTTTTTTCTTCTTTGATTCCTTTGCCAGAGAGAGAGTCACTTCATAGAGCCGGTGGAACTCCTTGGGGTGTTGGTTTGTTTCTTGTTGTTCTTCTTTTCATGGTTTCTTACCAATCTTCCTTTCAAGAACGTTGGTTTCCTTTACTCAGCAAGTGA

Protein sequence

>MsG0280009398.01.T02

MESIMDQVKMVLMISPLLLLLVVHFLSNYGDGGFFSSLIPLPERESLHRAGGTPWGVGLFLVVLLFMVSYQSSFQERWFPLLSK*

>MsG0280009398.01.T01

MATTHNSQPALPLHLCFFLLTLLIFLGFSWYSNYEPIMESIMDQVKMVLMISPLLLLLVVHFLSNYGDGGFFSSLIPLPERESLHRAGGTPWGVGLFLVVLLFMVSYQSSFQERWFPLLSK*