Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027572.01.T01 | XP_016577135.1 | 47.742 | 155 | 60 | 2 | 12 | 145 | 7 | 161 | 1.31E-32 | 123 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027572.01.T01 | A0A1U8H191 | 47.742 | 155 | 60 | 2 | 12 | 145 | 7 | 161 | 6.25e-33 | 123 |
| MsG0580027572.01.T01 | A0A2G3C6E9 | 47.742 | 155 | 60 | 2 | 12 | 145 | 88 | 242 | 3.71e-32 | 124 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005493.01 | MsG0580027572.01 | 0.823546 | 1.306676e-53 | 1.128488e-50 |
| MsG0480020242.01 | MsG0580027572.01 | 0.800229 | 1.589553e-48 | 7.362187e-46 |
| MsG0580027572.01 | MsG0780036773.01 | 0.803011 | 4.272713e-49 | 2.123619e-46 |
| MsG0580027572.01 | MsG0880043489.01 | 0.804148 | 2.482044e-49 | 1.270280e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027572.01.T01 | MTR_4g459420 | 74.172 | 151 | 8 | 2 | 24 | 145 | 18 | 166 | 4.90e-22 | 86.7 |
| MsG0580027572.01.T01 | MTR_4g059520 | 74.172 | 151 | 8 | 2 | 24 | 145 | 18 | 166 | 4.90e-22 | 86.7 |
| MsG0580027572.01.T01 | MTR_2g094170 | 43.210 | 81 | 41 | 2 | 67 | 145 | 71 | 148 | 1.81e-18 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027572.01.T01 | AT2G28440 | 42.683 | 82 | 44 | 1 | 67 | 145 | 187 | 268 | 6.16e-14 | 67.4 |
Find 42 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCGGTAGGTGTGGTTGCAT+TGG | 0.383469 | 5:+63494939 | MsG0580027572.01.T01:CDS |
| GAATTATCTGGTGAAGTTGC+CGG | 0.425458 | 5:-63494748 | None:intergenic |
| AATATTCAGAGATCTGAGTA+TGG | 0.433113 | 5:+63494991 | MsG0580027572.01.T01:CDS |
| GCGGTAGGTGTGGTTGCATT+GGG | 0.433302 | 5:+63494940 | MsG0580027572.01.T01:CDS |
| ATCGGTAAATTCTCCTTCTC+CGG | 0.435719 | 5:+63494729 | MsG0580027572.01.T01:CDS |
| TCCGGCGGAGGAATGAGTTC+AGG | 0.454233 | 5:+63494883 | MsG0580027572.01.T01:CDS |
| GAAGACGAAGGAGAATTATC+TGG | 0.454925 | 5:-63494760 | None:intergenic |
| CGCTTCATCCGCAGATTCTC+CGG | 0.464939 | 5:+63494678 | MsG0580027572.01.T01:CDS |
| TCCGGAGATGGCGCTGGTGA+TGG | 0.477898 | 5:-63494802 | None:intergenic |
| TCCGCCGCTTCGTCCGGAGA+TGG | 0.496131 | 5:-63494814 | None:intergenic |
| GCAATTAGCCACACCGGCAT+CGG | 0.502132 | 5:+63494844 | MsG0580027572.01.T01:CDS |
| GCCTGAACTCATTCCTCCGC+CGG | 0.515550 | 5:-63494884 | None:intergenic |
| ATGAGTTCAGGCAAGAAAGC+AGG | 0.515901 | 5:+63494895 | MsG0580027572.01.T01:CDS |
| ACCATCACCAGCGCCATCTC+CGG | 0.518919 | 5:+63494801 | MsG0580027572.01.T01:CDS |
| TCCGGCTCTGACTCCGACTC+CGG | 0.519151 | 5:+63494696 | MsG0580027572.01.T01:CDS |
| GGCGAAGACGGAAAATCGTC+CGG | 0.521635 | 5:+63494865 | MsG0580027572.01.T01:CDS |
| TCTGGTGAAGTTGCCGGAGA+AGG | 0.526071 | 5:-63494742 | None:intergenic |
| GACGGAAAATCGTCCGGCGG+AGG | 0.528964 | 5:+63494871 | MsG0580027572.01.T01:CDS |
| GCTTCGTCCGGAGATGGCGC+TGG | 0.531128 | 5:-63494808 | None:intergenic |
| GCCGGAGTCGGAGTCAGAGC+CGG | 0.540954 | 5:-63494697 | None:intergenic |
| CACACCGGCATCGGCGAAGA+CGG | 0.544834 | 5:+63494853 | MsG0580027572.01.T01:CDS |
| TGACTACGAATGAAAGAAGA+AGG | 0.559223 | 5:-63494651 | None:intergenic |
| GATGAATTCGTCGCCGGAGT+CGG | 0.570110 | 5:-63494709 | None:intergenic |
| GCCATCTCCGGACGAAGCGG+CGG | 0.575523 | 5:+63494813 | MsG0580027572.01.T01:CDS |
| TGTGGTTGCATTGGGAGCGA+TGG | 0.577207 | 5:+63494948 | MsG0580027572.01.T01:CDS |
| GTTGGAGTGATTGCGGCGGT+AGG | 0.577437 | 5:+63494925 | MsG0580027572.01.T01:CDS |
| AAGAAAGCAGGAATAGCGGT+TGG | 0.585656 | 5:+63494907 | MsG0580027572.01.T01:CDS |
| GGTTGCATTGGGAGCGATGG+TGG | 0.601732 | 5:+63494951 | MsG0580027572.01.T01:CDS |
| AGCGCCATCTCCGGACGAAG+CGG | 0.607521 | 5:+63494810 | MsG0580027572.01.T01:CDS |
| CGTCTTCGCCGATGCCGGTG+TGG | 0.612427 | 5:-63494852 | None:intergenic |
| GACTCCGGCGACGAATTCAT+CGG | 0.612594 | 5:+63494711 | MsG0580027572.01.T01:CDS |
| GATGGCGCTGGTGATGGTGT+AGG | 0.616515 | 5:-63494796 | None:intergenic |
| TTTACCGATGAATTCGTCGC+CGG | 0.617262 | 5:-63494715 | None:intergenic |
| GAAGGTGAAGGCGAAGACGA+AGG | 0.619327 | 5:-63494772 | None:intergenic |
| AATAGCGGTTGGAGTGATTG+CGG | 0.628826 | 5:+63494918 | MsG0580027572.01.T01:CDS |
| AGTCAGAGCCGGAGAATCTG+CGG | 0.637890 | 5:-63494686 | None:intergenic |
| AGTGATTGCGGCGGTAGGTG+TGG | 0.646980 | 5:+63494930 | MsG0580027572.01.T01:CDS |
| GCTGGTGATGGTGTAGGTGA+AGG | 0.655400 | 5:-63494790 | None:intergenic |
| AGGCAAGAAAGCAGGAATAG+CGG | 0.671962 | 5:+63494903 | MsG0580027572.01.T01:CDS |
| GATGGTGTAGGTGAAGGTGA+AGG | 0.694418 | 5:-63494784 | None:intergenic |
| GAAGACGGAAAATCGTCCGG+CGG | 0.717621 | 5:+63494868 | MsG0580027572.01.T01:CDS |
| AGCGGTTGGAGTGATTGCGG+CGG | 0.719476 | 5:+63494921 | MsG0580027572.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATATTCAGAGATCTGAGTA+TGG | + | Chr5:63494991-63495010 | MsG0580027572.01.T01:CDS | 30.0% | |
| TGACTACGAATGAAAGAAGA+AGG | - | Chr5:63494654-63494673 | None:intergenic | 35.0% | |
| AAAAACGCAATTAGCCACAC+CGG | + | Chr5:63494838-63494857 | MsG0580027572.01.T01:CDS | 40.0% | |
| ATCGGTAAATTCTCCTTCTC+CGG | + | Chr5:63494729-63494748 | MsG0580027572.01.T01:CDS | 40.0% | |
| GAAGACGAAGGAGAATTATC+TGG | - | Chr5:63494763-63494782 | None:intergenic | 40.0% | |
| GAATTATCTGGTGAAGTTGC+CGG | - | Chr5:63494751-63494770 | None:intergenic | 40.0% | |
| AAGAAAGCAGGAATAGCGGT+TGG | + | Chr5:63494907-63494926 | MsG0580027572.01.T01:CDS | 45.0% | |
| AATAGCGGTTGGAGTGATTG+CGG | + | Chr5:63494918-63494937 | MsG0580027572.01.T01:CDS | 45.0% | |
| AGGCAAGAAAGCAGGAATAG+CGG | + | Chr5:63494903-63494922 | MsG0580027572.01.T01:CDS | 45.0% | |
| ATGAGTTCAGGCAAGAAAGC+AGG | + | Chr5:63494895-63494914 | MsG0580027572.01.T01:CDS | 45.0% | |
| TTTACCGATGAATTCGTCGC+CGG | - | Chr5:63494718-63494737 | None:intergenic | 45.0% | |
| GATGGTGTAGGTGAAGGTGA+AGG | - | Chr5:63494787-63494806 | None:intergenic | 50.0% | |
| ! | TTTTTATCCGCCGCTTCGTC+CGG | - | Chr5:63494823-63494842 | None:intergenic | 50.0% |
| AGTCAGAGCCGGAGAATCTG+CGG | - | Chr5:63494689-63494708 | None:intergenic | 55.0% | |
| CGCTTCATCCGCAGATTCTC+CGG | + | Chr5:63494678-63494697 | MsG0580027572.01.T01:CDS | 55.0% | |
| GAAGACGGAAAATCGTCCGG+CGG | + | Chr5:63494868-63494887 | MsG0580027572.01.T01:CDS | 55.0% | |
| GAAGGTGAAGGCGAAGACGA+AGG | - | Chr5:63494775-63494794 | None:intergenic | 55.0% | |
| GACTCCGGCGACGAATTCAT+CGG | + | Chr5:63494711-63494730 | MsG0580027572.01.T01:CDS | 55.0% | |
| GATGAATTCGTCGCCGGAGT+CGG | - | Chr5:63494712-63494731 | None:intergenic | 55.0% | |
| GCAATTAGCCACACCGGCAT+CGG | + | Chr5:63494844-63494863 | MsG0580027572.01.T01:CDS | 55.0% | |
| GCGGTAGGTGTGGTTGCATT+GGG | + | Chr5:63494940-63494959 | MsG0580027572.01.T01:CDS | 55.0% | |
| GGCGAAGACGGAAAATCGTC+CGG | + | Chr5:63494865-63494884 | MsG0580027572.01.T01:CDS | 55.0% | |
| TCTGGTGAAGTTGCCGGAGA+AGG | - | Chr5:63494745-63494764 | None:intergenic | 55.0% | |
| TGTGGTTGCATTGGGAGCGA+TGG | + | Chr5:63494948-63494967 | MsG0580027572.01.T01:CDS | 55.0% | |
| ! | TTTTCCGTCTTCGCCGATGC+CGG | - | Chr5:63494860-63494879 | None:intergenic | 55.0% |
| !! | GCTGGTGATGGTGTAGGTGA+AGG | - | Chr5:63494793-63494812 | None:intergenic | 55.0% |
| ACCATCACCAGCGCCATCTC+CGG | + | Chr5:63494801-63494820 | MsG0580027572.01.T01:CDS | 60.0% | |
| AGCGGTTGGAGTGATTGCGG+CGG | + | Chr5:63494921-63494940 | MsG0580027572.01.T01:CDS | 60.0% | |
| AGTGATTGCGGCGGTAGGTG+TGG | + | Chr5:63494930-63494949 | MsG0580027572.01.T01:CDS | 60.0% | |
| GCCTGAACTCATTCCTCCGC+CGG | - | Chr5:63494887-63494906 | None:intergenic | 60.0% | |
| GGCGGTAGGTGTGGTTGCAT+TGG | + | Chr5:63494939-63494958 | MsG0580027572.01.T01:CDS | 60.0% | |
| GGTTGCATTGGGAGCGATGG+TGG | + | Chr5:63494951-63494970 | MsG0580027572.01.T01:CDS | 60.0% | |
| GTTGGAGTGATTGCGGCGGT+AGG | + | Chr5:63494925-63494944 | MsG0580027572.01.T01:CDS | 60.0% | |
| TCCGGCGGAGGAATGAGTTC+AGG | + | Chr5:63494883-63494902 | MsG0580027572.01.T01:CDS | 60.0% | |
| !! | GATGGCGCTGGTGATGGTGT+AGG | - | Chr5:63494799-63494818 | None:intergenic | 60.0% |
| AGCGCCATCTCCGGACGAAG+CGG | + | Chr5:63494810-63494829 | MsG0580027572.01.T01:CDS | 65.0% | |
| GACGGAAAATCGTCCGGCGG+AGG | + | Chr5:63494871-63494890 | MsG0580027572.01.T01:CDS | 65.0% | |
| ! | TCCGGCTCTGACTCCGACTC+CGG | + | Chr5:63494696-63494715 | MsG0580027572.01.T01:CDS | 65.0% |
| !! | CACACCGGCATCGGCGAAGA+CGG | + | Chr5:63494853-63494872 | MsG0580027572.01.T01:CDS | 65.0% |
| !! | TCCGGAGATGGCGCTGGTGA+TGG | - | Chr5:63494805-63494824 | None:intergenic | 65.0% |
| CGTCTTCGCCGATGCCGGTG+TGG | - | Chr5:63494855-63494874 | None:intergenic | 70.0% | |
| GCCATCTCCGGACGAAGCGG+CGG | + | Chr5:63494813-63494832 | MsG0580027572.01.T01:CDS | 70.0% | |
| GCCGGAGTCGGAGTCAGAGC+CGG | - | Chr5:63494700-63494719 | None:intergenic | 70.0% | |
| GCTTCGTCCGGAGATGGCGC+TGG | - | Chr5:63494811-63494830 | None:intergenic | 70.0% | |
| TCCGCCGCTTCGTCCGGAGA+TGG | - | Chr5:63494817-63494836 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 63494604 | 63495041 | 63494604 | ID=MsG0580027572.01;Name=MsG0580027572.01 |
| Chr5 | mRNA | 63494604 | 63495041 | 63494604 | ID=MsG0580027572.01.T01;Parent=MsG0580027572.01;Name=MsG0580027572.01.T01;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|0|1|0|145 |
| Chr5 | exon | 63494604 | 63495041 | 63494604 | ID=MsG0580027572.01.T01:exon:37367;Parent=MsG0580027572.01.T01 |
| Chr5 | CDS | 63494604 | 63495041 | 63494604 | ID=MsG0580027572.01.T01:cds;Parent=MsG0580027572.01.T01 |
| Gene Sequence |
| Protein sequence |
