AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043489.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043489.01.T01 MTR_8g036980 96.711 152 4 1 1 152 1 151 1.08e-105 298
MsG0880043489.01.T01 MTR_3g028010 45.509 167 72 4 1 152 1 163 8.21e-41 134
MsG0880043489.01.T01 MTR_4g065570 55.405 74 33 0 79 152 64 137 1.48e-27 100
MsG0880043489.01.T01 MTR_2g098160 65.574 61 21 0 92 152 112 172 4.46e-27 99.8
MsG0880043489.01.T01 MTR_7g092400 50.602 83 41 0 70 152 178 260 1.71e-26 100
MsG0880043489.01.T01 MTR_0249s0070 53.425 73 34 0 80 152 97 169 2.25e-26 98.2
MsG0880043489.01.T01 MTR_0249s0070 53.425 73 34 0 80 152 105 177 2.84e-26 97.8
MsG0880043489.01.T01 MTR_1g060380 58.065 62 26 0 91 152 95 156 8.85e-24 90.9
MsG0880043489.01.T01 MTR_7g104320 56.452 62 27 0 91 152 107 168 1.76e-23 90.5
MsG0880043489.01.T01 MTR_4g053440 53.226 62 29 0 91 152 109 170 1.25e-21 85.9
MsG0880043489.01.T01 MTR_2g086190 50.000 62 31 0 91 152 89 150 7.55e-21 83.2
MsG0880043489.01.T01 MTR_2g054650 47.436 78 38 1 68 142 40 117 2.89e-20 81.3
MsG0880043489.01.T01 MTR_1g100773 35.514 107 60 2 52 149 31 137 7.53e-18 75.5
MsG0880043489.01.T01 MTR_7g114920 46.269 67 36 0 83 149 64 130 1.22e-17 74.7
MsG0880043489.01.T01 MTR_3g025570 66.667 45 15 0 86 130 3 47 5.42e-16 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043489.01.T01 AT3G12630 50.303 165 64 6 1 152 1 160 7.57e-42 137
MsG0880043489.01.T01 AT3G52800 59.722 72 29 0 81 152 99 170 8.03e-27 99.4
MsG0880043489.01.T01 AT3G52800 59.722 72 29 0 81 152 99 170 8.03e-27 99.4
MsG0880043489.01.T01 AT2G36320 61.290 62 24 0 91 152 100 161 3.22e-24 92.4
MsG0880043489.01.T01 AT2G27580 47.191 89 39 1 64 152 83 163 1.19e-23 90.9
MsG0880043489.01.T01 AT2G27580 47.191 89 39 1 64 152 83 163 1.19e-23 90.9
MsG0880043489.01.T01 AT4G22820 56.452 62 27 0 91 152 115 176 7.44e-23 89.4
MsG0880043489.01.T01 AT4G22820 56.452 62 27 0 91 152 115 176 7.44e-23 89.4
MsG0880043489.01.T01 AT4G12040 46.667 75 40 0 78 152 101 175 2.75e-22 87.8
MsG0880043489.01.T01 AT4G12040 46.667 75 40 0 78 152 101 175 2.75e-22 87.8
MsG0880043489.01.T01 AT1G51200 62.295 61 23 0 92 152 113 173 1.01e-20 83.6
MsG0880043489.01.T01 AT1G51200 62.295 61 23 0 92 152 113 173 1.01e-20 83.6
MsG0880043489.01.T01 AT1G51200 62.295 61 23 0 92 152 113 173 1.01e-20 83.6
MsG0880043489.01.T01 AT1G51200 62.295 61 23 0 92 152 113 173 1.01e-20 83.6
MsG0880043489.01.T01 AT1G12440 48.485 66 34 0 87 152 103 168 1.18e-20 83.2
MsG0880043489.01.T01 AT1G12440 48.485 66 34 0 87 152 103 168 1.18e-20 83.2
MsG0880043489.01.T01 AT1G12440 48.485 66 34 0 87 152 103 168 1.18e-20 83.2
MsG0880043489.01.T01 AT4G25380 38.202 89 52 1 67 152 42 130 1.06e-19 79.7
MsG0880043489.01.T01 AT4G14225 50.877 57 28 0 96 152 69 125 1.44e-16 71.6

Find 27 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTGGTGTTATTGTTTC+AGG 0.174761 8:+25490757 None:intergenic
GCTTCTGACGACGTCGTTTG+AGG 0.385840 8:+25490568 None:intergenic
GGTGTTGTTGTTGTTTGTGT+TGG 0.387804 8:+25490727 None:intergenic
GTGTTATTGTTTCAGGAACT+TGG 0.395112 8:+25490764 None:intergenic
ATCTTCGCAGCTTTAACAAT+AGG 0.400791 8:+25490379 None:intergenic
AAACGATGACGTGGCAGATC+TGG 0.402657 8:+25490636 None:intergenic
ACCGCATCGGCAACGGAATC+CGG 0.406728 8:+25490495 None:intergenic
TTGTCTTCAAAGAATCTAGA+TGG 0.406835 8:+25490670 None:intergenic
GTGTTGTTGTTGTTTGTGTT+GGG 0.411064 8:+25490728 None:intergenic
AGGAAGAGAGTCGGTCTTAC+CGG 0.411854 8:-25490514 MsG0880043489.01.T01:CDS
GTTGTTGTTGTGGTTGTTGT+TGG 0.430807 8:+25490703 None:intergenic
TGAATCGTTGCTCCGGTTGT+AGG 0.436602 8:-25490534 MsG0880043489.01.T01:CDS
ACCGACTCTCTTCCTACAAC+CGG 0.452390 8:+25490522 None:intergenic
TCGTATGATTATAAAGCTGC+TGG 0.466775 8:-25490427 MsG0880043489.01.T01:CDS
CGCGCCGTGAATCGTTGCTC+CGG 0.486851 8:-25490541 MsG0880043489.01.T01:CDS
GTTTGTGTTGGGTTGATGCA+TGG 0.490623 8:+25490739 None:intergenic
AAAGATCACCGCATCGGCAA+CGG 0.503629 8:+25490488 None:intergenic
GTTGTTGTGGTTGTTGTTGG+TGG 0.520962 8:+25490706 None:intergenic
AGATCTGAATCATCGTTGCT+TGG 0.523634 8:+25490595 None:intergenic
TTCTGTTAACGTTGTTGTTG+TGG 0.526236 8:+25490693 None:intergenic
ACAACCGGAGCAACGATTCA+CGG 0.527957 8:+25490537 None:intergenic
ACCGGATTCCGTTGCCGATG+CGG 0.581520 8:-25490496 MsG0880043489.01.T01:CDS
TGTGTTGGGTTGATGCATGG+TGG 0.586051 8:+25490742 None:intergenic
TCCGGTTGTAGGAAGAGAGT+CGG 0.612838 8:-25490523 MsG0880043489.01.T01:CDS
AGCAGAAAAGATCACCGCAT+CGG 0.632703 8:+25490482 None:intergenic
ATACGAACAATCATGACGAT+CGG 0.643205 8:+25490444 None:intergenic
ACGATCGGAGTATCGATGCT+CGG 0.648397 8:+25490459 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTCTGTTTCTTCTTTTT+TGG + Chr8:25490385-25490404 None:intergenic 25.0%
TTGTCTTCAAAGAATCTAGA+TGG + Chr8:25490501-25490520 None:intergenic 30.0%
ATACGAACAATCATGACGAT+CGG + Chr8:25490727-25490746 None:intergenic 35.0%
TCGTATGATTATAAAGCTGC+TGG - Chr8:25490741-25490760 MsG0880043489.01.T01:CDS 35.0%
! ATCTTCGCAGCTTTAACAAT+AGG + Chr8:25490792-25490811 None:intergenic 35.0%
! GTGTTATTGTTTCAGGAACT+TGG + Chr8:25490407-25490426 None:intergenic 35.0%
! TTCTGTTAACGTTGTTGTTG+TGG + Chr8:25490478-25490497 None:intergenic 35.0%
!! GTGTTGTTGTTGTTTGTGTT+GGG + Chr8:25490443-25490462 None:intergenic 35.0%
AGATCTGAATCATCGTTGCT+TGG + Chr8:25490576-25490595 None:intergenic 40.0%
! CTTTTGTGAAAACGATGACG+TGG + Chr8:25490544-25490563 None:intergenic 40.0%
! GTTGTTGTTGTGGTTGTTGT+TGG + Chr8:25490468-25490487 None:intergenic 40.0%
!! CATGGTGGTGTTATTGTTTC+AGG + Chr8:25490414-25490433 None:intergenic 40.0%
!! GGTGTTGTTGTTGTTTGTGT+TGG + Chr8:25490444-25490463 None:intergenic 40.0%
AGCAGAAAAGATCACCGCAT+CGG + Chr8:25490689-25490708 None:intergenic 45.0%
! GTTGTTGTGGTTGTTGTTGG+TGG + Chr8:25490465-25490484 None:intergenic 45.0%
!! GTTTGTGTTGGGTTGATGCA+TGG + Chr8:25490432-25490451 None:intergenic 45.0%
AAACGATGACGTGGCAGATC+TGG + Chr8:25490535-25490554 None:intergenic 50.0%
ACAACCGGAGCAACGATTCA+CGG + Chr8:25490634-25490653 None:intergenic 50.0%
AGGAAGAGAGTCGGTCTTAC+CGG - Chr8:25490654-25490673 MsG0880043489.01.T01:CDS 50.0%
TCCGGTTGTAGGAAGAGAGT+CGG - Chr8:25490645-25490664 MsG0880043489.01.T01:CDS 50.0%
TGAATCGTTGCTCCGGTTGT+AGG - Chr8:25490634-25490653 MsG0880043489.01.T01:CDS 50.0%
! AAAGATCACCGCATCGGCAA+CGG + Chr8:25490683-25490702 None:intergenic 50.0%
! ACCGACTCTCTTCCTACAAC+CGG + Chr8:25490649-25490668 None:intergenic 50.0%
!! ACGATCGGAGTATCGATGCT+CGG + Chr8:25490712-25490731 None:intergenic 50.0%
!! TGTGTTGGGTTGATGCATGG+TGG + Chr8:25490429-25490448 None:intergenic 50.0%
GCTTCTGACGACGTCGTTTG+AGG + Chr8:25490603-25490622 None:intergenic 55.0%
ACCGGATTCCGTTGCCGATG+CGG - Chr8:25490672-25490691 MsG0880043489.01.T01:CDS 60.0%
! ACCGCATCGGCAACGGAATC+CGG + Chr8:25490676-25490695 None:intergenic 60.0%
CGCGCCGTGAATCGTTGCTC+CGG - Chr8:25490627-25490646 MsG0880043489.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 25490366 25490824 25490366 ID=MsG0880043489.01;Name=MsG0880043489.01
Chr8 mRNA 25490366 25490824 25490366 ID=MsG0880043489.01.T01;Parent=MsG0880043489.01;Name=MsG0880043489.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|152
Chr8 exon 25490366 25490824 25490366 ID=MsG0880043489.01.T01:exon:1181;Parent=MsG0880043489.01.T01
Chr8 CDS 25490366 25490824 25490366 ID=MsG0880043489.01.T01:cds;Parent=MsG0880043489.01.T01
Gene Sequence

>MsG0880043489.01.T01

ATGGCTCACAAAATAACCAAAAAAGAAGAAACAGAATTCCAAGTTCCTGAAACAATAACACCACCATGCATCAACCCAACACAAACAACAACAACACCACCAACAACAACCACAACAACAACGTTAACAGAACCATCTAGATTCTTTGAAGACAAATCTTCTAAAGCCAGATCTGCCACGTCATCGTTTTCACAAAAGAGATCTCATCCAAGCAACGATGATTCAGATCTTCAGCCTCAAACGACGTCGTCAGAAGCAAAACGCGCCGTGAATCGTTGCTCCGGTTGTAGGAAGAGAGTCGGTCTTACCGGATTCCGTTGCCGATGCGGTGATCTTTTCTGCTCCGAGCATCGATACTCCGATCGTCATGATTGTTCGTATGATTATAAAGCTGCTGGAAGAGTATCTATTGCTAGAGAAAATCCTATTGTTAAAGCTGCGAAGATTGTTAAGCTCTGA

Protein sequence

>MsG0880043489.01.T01

MAHKITKKEETEFQVPETITPPCINPTQTTTTPPTTTTTTTLTEPSRFFEDKSSKARSATSSFSQKRSHPSNDDSDLQPQTTSSEAKRAVNRCSGCRKRVGLTGFRCRCGDLFCSEHRYSDRHDCSYDYKAAGRVSIARENPIVKAAKIVKL*