Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027986.01.T01 | GAU15862.1 | 45.545 | 101 | 42 | 2 | 31 | 125 | 172 | 265 | 1.04E-13 | 76.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580026422.01 | MsG0580027986.01 | 0.925109 | 2.861599e-90 | 1.253798e-85 |
| MsG0580027687.01 | MsG0580027986.01 | 0.817151 | 3.825354e-52 | 2.764073e-49 |
| MsG0580027688.01 | MsG0580027986.01 | 0.810664 | 1.029875e-50 | 6.245802e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGACAAATCGCTTGATTT+TGG | 0.221118 | 5:-72459201 | MsG0580027986.01.T01:CDS |
| TTCTTTCTTAGCATCTTTCT+TGG | 0.275859 | 5:+72459466 | None:intergenic |
| GAATGTGTTGAATAGGTTAA+TGG | 0.333245 | 5:-72459324 | MsG0580027986.01.T01:CDS |
| TGTGAATACAGCGTCTCTTC+TGG | 0.334946 | 5:+72459404 | None:intergenic |
| GTCTGCAGCGGCGGCGGCGG+CGG | 0.349174 | 5:+72459520 | None:intergenic |
| GGGATCGGAGAAGGAAGAAT+TGG | 0.406847 | 5:-72459291 | MsG0580027986.01.T01:CDS |
| TACAGCGTCTCTTCTGGCCT+CGG | 0.413632 | 5:+72459410 | None:intergenic |
| GCGGGAATGTAGTTATCAAA+TGG | 0.427127 | 5:-72459366 | MsG0580027986.01.T01:CDS |
| TAGATATGAATGTGTTGAAT+AGG | 0.453928 | 5:-72459331 | MsG0580027986.01.T01:CDS |
| TGAGTCTGCAGCGGCGGCGG+CGG | 0.455416 | 5:+72459517 | None:intergenic |
| TGATGAGTCTGCAGCGGCGG+CGG | 0.481622 | 5:+72459514 | None:intergenic |
| CGGGAATGTAGTTATCAAAT+GGG | 0.483193 | 5:-72459365 | MsG0580027986.01.T01:CDS |
| CATCAAACCGGCCGCGTAAG+AGG | 0.488792 | 5:-72459496 | MsG0580027986.01.T01:CDS |
| AGAAGGAAGAATTGGAAAGA+AGG | 0.489922 | 5:-72459283 | MsG0580027986.01.T01:CDS |
| TCAAGAGTTGAAACTGTTTG+TGG | 0.491313 | 5:-72459243 | MsG0580027986.01.T01:CDS |
| AATGGAATCGATTGAGGGAT+CGG | 0.493309 | 5:-72459306 | MsG0580027986.01.T01:CDS |
| ACAGGAAGAATTTGTGAAGC+GGG | 0.513907 | 5:-72459384 | MsG0580027986.01.T01:CDS |
| AGGAAGAATTGGAAAGAAGG+TGG | 0.531844 | 5:-72459280 | MsG0580027986.01.T01:CDS |
| CCGCTGCAGACTCATCAAAC+CGG | 0.534109 | 5:-72459508 | MsG0580027986.01.T01:CDS |
| ATCGATTGAGGGATCGGAGA+AGG | 0.536386 | 5:-72459300 | MsG0580027986.01.T01:CDS |
| AGAAGAGACGCTGTATTCAC+AGG | 0.547380 | 5:-72459402 | MsG0580027986.01.T01:CDS |
| TAGGTTAATGGAATCGATTG+AGG | 0.553633 | 5:-72459312 | MsG0580027986.01.T01:CDS |
| TAAAGCGCCTCTTACGCGGC+CGG | 0.553884 | 5:+72459489 | None:intergenic |
| AGGTTAATGGAATCGATTGA+GGG | 0.560272 | 5:-72459311 | MsG0580027986.01.T01:CDS |
| CACAGGAAGAATTTGTGAAG+CGG | 0.579131 | 5:-72459385 | MsG0580027986.01.T01:CDS |
| GTTTGATGAGTCTGCAGCGG+CGG | 0.580694 | 5:+72459511 | None:intergenic |
| AGAGTTGAAACTGTTTGTGG+AGG | 0.602889 | 5:-72459240 | MsG0580027986.01.T01:CDS |
| TTGGTAAAGCGCCTCTTACG+CGG | 0.641927 | 5:+72459485 | None:intergenic |
| CCGGTTTGATGAGTCTGCAG+CGG | 0.651993 | 5:+72459508 | None:intergenic |
| AAAATGCTGCTAAGAAACCG+AGG | 0.699686 | 5:-72459427 | MsG0580027986.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAGATATGAATGTGTTGAAT+AGG | - | Chr5:72459386-72459405 | MsG0580027986.01.T01:CDS | 25.0% |
| GAATGTGTTGAATAGGTTAA+TGG | - | Chr5:72459393-72459412 | MsG0580027986.01.T01:CDS | 30.0% | |
| TTCTTTCTTAGCATCTTTCT+TGG | + | Chr5:72459254-72459273 | None:intergenic | 30.0% | |
| AGAAGGAAGAATTGGAAAGA+AGG | - | Chr5:72459434-72459453 | MsG0580027986.01.T01:CDS | 35.0% | |
| AGGTTAATGGAATCGATTGA+GGG | - | Chr5:72459406-72459425 | MsG0580027986.01.T01:CDS | 35.0% | |
| CGGGAATGTAGTTATCAAAT+GGG | - | Chr5:72459352-72459371 | MsG0580027986.01.T01:CDS | 35.0% | |
| GAAAGAAAAATCACCCAACA+AGG | - | Chr5:72459267-72459286 | MsG0580027986.01.T01:CDS | 35.0% | |
| TAGGTTAATGGAATCGATTG+AGG | - | Chr5:72459405-72459424 | MsG0580027986.01.T01:CDS | 35.0% | |
| TCAAGAGTTGAAACTGTTTG+TGG | - | Chr5:72459474-72459493 | MsG0580027986.01.T01:CDS | 35.0% | |
| ! | AGAGACAAATCGCTTGATTT+TGG | - | Chr5:72459516-72459535 | MsG0580027986.01.T01:CDS | 35.0% |
| ! | TTAGCAGCATTTTCCTTGTT+GGG | + | Chr5:72459283-72459302 | None:intergenic | 35.0% |
| AAAATGCTGCTAAGAAACCG+AGG | - | Chr5:72459290-72459309 | MsG0580027986.01.T01:CDS | 40.0% | |
| AATGGAATCGATTGAGGGAT+CGG | - | Chr5:72459411-72459430 | MsG0580027986.01.T01:CDS | 40.0% | |
| ACAGGAAGAATTTGTGAAGC+GGG | - | Chr5:72459333-72459352 | MsG0580027986.01.T01:CDS | 40.0% | |
| AGAGTTGAAACTGTTTGTGG+AGG | - | Chr5:72459477-72459496 | MsG0580027986.01.T01:CDS | 40.0% | |
| AGGAAGAATTGGAAAGAAGG+TGG | - | Chr5:72459437-72459456 | MsG0580027986.01.T01:CDS | 40.0% | |
| CACAGGAAGAATTTGTGAAG+CGG | - | Chr5:72459332-72459351 | MsG0580027986.01.T01:CDS | 40.0% | |
| GCGGGAATGTAGTTATCAAA+TGG | - | Chr5:72459351-72459370 | MsG0580027986.01.T01:CDS | 40.0% | |
| ! | CTTAGCAGCATTTTCCTTGT+TGG | + | Chr5:72459284-72459303 | None:intergenic | 40.0% |
| ! | GACAAATCGCTTGATTTTGG+AGG | - | Chr5:72459519-72459538 | MsG0580027986.01.T01:CDS | 40.0% |
| AGAAGAGACGCTGTATTCAC+AGG | - | Chr5:72459315-72459334 | MsG0580027986.01.T01:CDS | 45.0% | |
| TGTGAATACAGCGTCTCTTC+TGG | + | Chr5:72459316-72459335 | None:intergenic | 45.0% | |
| !! | ATCGCTTGATTTTGGAGGCA+TGG | - | Chr5:72459524-72459543 | MsG0580027986.01.T01:CDS | 45.0% |
| ATCGATTGAGGGATCGGAGA+AGG | - | Chr5:72459417-72459436 | MsG0580027986.01.T01:CDS | 50.0% | |
| GGGATCGGAGAAGGAAGAAT+TGG | - | Chr5:72459426-72459445 | MsG0580027986.01.T01:CDS | 50.0% | |
| ! | TTGGTAAAGCGCCTCTTACG+CGG | + | Chr5:72459235-72459254 | None:intergenic | 50.0% |
| !! | TTGATTTTGGAGGCATGGCG+AGG | - | Chr5:72459529-72459548 | MsG0580027986.01.T01:CDS | 50.0% |
| CCGCTGCAGACTCATCAAAC+CGG | - | Chr5:72459209-72459228 | MsG0580027986.01.T01:CDS | 55.0% | |
| GTTTGATGAGTCTGCAGCGG+CGG | + | Chr5:72459209-72459228 | None:intergenic | 55.0% | |
| TACAGCGTCTCTTCTGGCCT+CGG | + | Chr5:72459310-72459329 | None:intergenic | 55.0% | |
| ! | CCGGTTTGATGAGTCTGCAG+CGG | + | Chr5:72459212-72459231 | None:intergenic | 55.0% |
| CATCAAACCGGCCGCGTAAG+AGG | - | Chr5:72459221-72459240 | MsG0580027986.01.T01:CDS | 60.0% | |
| ! | TAAAGCGCCTCTTACGCGGC+CGG | + | Chr5:72459231-72459250 | None:intergenic | 60.0% |
| TGATGAGTCTGCAGCGGCGG+CGG | + | Chr5:72459206-72459225 | None:intergenic | 65.0% | |
| TGAGTCTGCAGCGGCGGCGG+CGG | + | Chr5:72459203-72459222 | None:intergenic | 75.0% | |
| ! | GTCTGCAGCGGCGGCGGCGG+CGG | + | Chr5:72459200-72459219 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 72459178 | 72459561 | 72459178 | ID=MsG0580027986.01;Name=MsG0580027986.01 |
| Chr5 | mRNA | 72459178 | 72459561 | 72459178 | ID=MsG0580027986.01.T01;Parent=MsG0580027986.01;Name=MsG0580027986.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|127 |
| Chr5 | exon | 72459178 | 72459561 | 72459178 | ID=MsG0580027986.01.T01:exon:4606;Parent=MsG0580027986.01.T01 |
| Chr5 | CDS | 72459178 | 72459561 | 72459178 | ID=MsG0580027986.01.T01:cds;Parent=MsG0580027986.01.T01 |
| Gene Sequence |
| Protein sequence |
