Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026422.01.T01 | GAU15862.1 | 46.535 | 101 | 41 | 2 | 30 | 124 | 172 | 265 | 1.45E-15 | 81.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026422.01.T01 | A0A1S3DYV7 | 42.975 | 121 | 45 | 3 | 30 | 126 | 414 | 534 | 2.11e-11 | 70.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380011870.01 | MsG0580026422.01 | 0.830954 | 2.195103e-55 | 2.348090e-52 |
| MsG0380012939.01 | MsG0580026422.01 | 0.825922 | 3.598340e-54 | 3.325526e-51 |
| MsG0580026422.01 | MsG0580027687.01 | 0.852407 | 4.712155e-61 | 9.836258e-58 |
| MsG0580026422.01 | MsG0580027688.01 | 0.844134 | 9.124558e-59 | 1.459181e-55 |
| MsG0580026422.01 | MsG0580027986.01 | 0.925109 | 2.861599e-90 | 1.253798e-85 |
| MsG0580026422.01 | MsG0580028000.01 | 0.801829 | 7.486882e-49 | 3.611049e-46 |
| MsG0580026422.01 | MsG0580029500.01 | -0.811108 | 8.256360e-51 | 5.066430e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGACAAATCGCTTGATTT+TGG | 0.221118 | 5:-36982610 | MsG0580026422.01.T01:CDS |
| TTCTTTCTTAGCATCTTTCT+TGG | 0.275859 | 5:+36982875 | None:intergenic |
| TGTGAATACAGTGTCTCTTC+TGG | 0.330987 | 5:+36982813 | None:intergenic |
| GAATGTGTTGAATAGGTTAA+TGG | 0.333245 | 5:-36982733 | MsG0580026422.01.T01:CDS |
| TACAGTGTCTCTTCTGGCCT+CGG | 0.375247 | 5:+36982819 | None:intergenic |
| GGGATCGGAGAAGGAAGAAT+TGG | 0.406847 | 5:-36982700 | MsG0580026422.01.T01:CDS |
| TAGATATGAATGTGTTGAAT+AGG | 0.453928 | 5:-36982740 | MsG0580026422.01.T01:CDS |
| TGAGTCTGCAGCGGCGGCGG+CGG | 0.455416 | 5:+36982926 | None:intergenic |
| TGATGAGTCTGCAGCGGCGG+CGG | 0.481622 | 5:+36982923 | None:intergenic |
| GGGGAATGTAGTTATCAAAT+GGG | 0.485794 | 5:-36982774 | MsG0580026422.01.T01:CDS |
| CATCAAACCGGCCGCGTAAG+AGG | 0.488792 | 5:-36982905 | MsG0580026422.01.T01:CDS |
| AGAAGGAAGAATTGGAAAGA+AGG | 0.489922 | 5:-36982692 | MsG0580026422.01.T01:CDS |
| AATGGAATCGATTGAGGGAT+CGG | 0.493309 | 5:-36982715 | MsG0580026422.01.T01:CDS |
| AGGAAGAATTGGAAAGAAGG+TGG | 0.531844 | 5:-36982689 | MsG0580026422.01.T01:CDS |
| CCGCTGCAGACTCATCAAAC+CGG | 0.534109 | 5:-36982917 | MsG0580026422.01.T01:CDS |
| ATCGATTGAGGGATCGGAGA+AGG | 0.536386 | 5:-36982709 | MsG0580026422.01.T01:CDS |
| TAGGTTAATGGAATCGATTG+AGG | 0.553633 | 5:-36982721 | MsG0580026422.01.T01:CDS |
| TAAAGCGCCTCTTACGCGGC+CGG | 0.553884 | 5:+36982898 | None:intergenic |
| AGGTTAATGGAATCGATTGA+GGG | 0.560272 | 5:-36982720 | MsG0580026422.01.T01:CDS |
| AGAAGAGACACTGTATTCAC+AGG | 0.572182 | 5:-36982811 | MsG0580026422.01.T01:CDS |
| CACAGGAAGAATTTGTGAAG+GGG | 0.572656 | 5:-36982794 | MsG0580026422.01.T01:CDS |
| TCAAGAGTTGAAACTGTTTG+TGG | 0.576038 | 5:-36982652 | MsG0580026422.01.T01:CDS |
| GTTTGATGAGTCTGCAGCGG+CGG | 0.580694 | 5:+36982920 | None:intergenic |
| TTCACAGGAAGAATTTGTGA+AGG | 0.593335 | 5:-36982796 | MsG0580026422.01.T01:CDS |
| TCACAGGAAGAATTTGTGAA+GGG | 0.599562 | 5:-36982795 | MsG0580026422.01.T01:CDS |
| AGAGTTGAAACTGTTTGTGG+TGG | 0.616000 | 5:-36982649 | MsG0580026422.01.T01:CDS |
| ACAGGAAGAATTTGTGAAGG+GGG | 0.620371 | 5:-36982793 | MsG0580026422.01.T01:CDS |
| TTGGTAAAGCGCCTCTTACG+CGG | 0.641927 | 5:+36982894 | None:intergenic |
| CCGGTTTGATGAGTCTGCAG+CGG | 0.651993 | 5:+36982917 | None:intergenic |
| AAAATGCTGCTAAGAAACCG+AGG | 0.699686 | 5:-36982836 | MsG0580026422.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAGATATGAATGTGTTGAAT+AGG | - | Chr5:36982792-36982811 | MsG0580026422.01.T01:CDS | 25.0% |
| GAATGTGTTGAATAGGTTAA+TGG | - | Chr5:36982799-36982818 | MsG0580026422.01.T01:CDS | 30.0% | |
| TTCTTTCTTAGCATCTTTCT+TGG | + | Chr5:36982660-36982679 | None:intergenic | 30.0% | |
| AGAAGGAAGAATTGGAAAGA+AGG | - | Chr5:36982840-36982859 | MsG0580026422.01.T01:CDS | 35.0% | |
| AGGTTAATGGAATCGATTGA+GGG | - | Chr5:36982812-36982831 | MsG0580026422.01.T01:CDS | 35.0% | |
| GAAAGAAAAATCACCCAACA+AGG | - | Chr5:36982673-36982692 | MsG0580026422.01.T01:CDS | 35.0% | |
| GGGGAATGTAGTTATCAAAT+GGG | - | Chr5:36982758-36982777 | MsG0580026422.01.T01:CDS | 35.0% | |
| TAGGTTAATGGAATCGATTG+AGG | - | Chr5:36982811-36982830 | MsG0580026422.01.T01:CDS | 35.0% | |
| TCAAGAGTTGAAACTGTTTG+TGG | - | Chr5:36982880-36982899 | MsG0580026422.01.T01:CDS | 35.0% | |
| TCACAGGAAGAATTTGTGAA+GGG | - | Chr5:36982737-36982756 | MsG0580026422.01.T01:CDS | 35.0% | |
| TTCACAGGAAGAATTTGTGA+AGG | - | Chr5:36982736-36982755 | MsG0580026422.01.T01:CDS | 35.0% | |
| ! | AGAGACAAATCGCTTGATTT+TGG | - | Chr5:36982922-36982941 | MsG0580026422.01.T01:CDS | 35.0% |
| ! | TTAGCAGCATTTTCCTTGTT+GGG | + | Chr5:36982689-36982708 | None:intergenic | 35.0% |
| AAAATGCTGCTAAGAAACCG+AGG | - | Chr5:36982696-36982715 | MsG0580026422.01.T01:CDS | 40.0% | |
| AATGGAATCGATTGAGGGAT+CGG | - | Chr5:36982817-36982836 | MsG0580026422.01.T01:CDS | 40.0% | |
| ACAGGAAGAATTTGTGAAGG+GGG | - | Chr5:36982739-36982758 | MsG0580026422.01.T01:CDS | 40.0% | |
| AGAAGAGACACTGTATTCAC+AGG | - | Chr5:36982721-36982740 | MsG0580026422.01.T01:CDS | 40.0% | |
| AGAGTTGAAACTGTTTGTGG+TGG | - | Chr5:36982883-36982902 | MsG0580026422.01.T01:CDS | 40.0% | |
| AGGAAGAATTGGAAAGAAGG+TGG | - | Chr5:36982843-36982862 | MsG0580026422.01.T01:CDS | 40.0% | |
| CACAGGAAGAATTTGTGAAG+GGG | - | Chr5:36982738-36982757 | MsG0580026422.01.T01:CDS | 40.0% | |
| GGGGGAATGTAGTTATCAAA+TGG | - | Chr5:36982757-36982776 | MsG0580026422.01.T01:CDS | 40.0% | |
| TGTGAATACAGTGTCTCTTC+TGG | + | Chr5:36982722-36982741 | None:intergenic | 40.0% | |
| ! | CTTAGCAGCATTTTCCTTGT+TGG | + | Chr5:36982690-36982709 | None:intergenic | 40.0% |
| ! | GACAAATCGCTTGATTTTGG+AGG | - | Chr5:36982925-36982944 | MsG0580026422.01.T01:CDS | 40.0% |
| !! | ATCGCTTGATTTTGGAGGCA+TGG | - | Chr5:36982930-36982949 | MsG0580026422.01.T01:CDS | 45.0% |
| ATCGATTGAGGGATCGGAGA+AGG | - | Chr5:36982823-36982842 | MsG0580026422.01.T01:CDS | 50.0% | |
| GGGATCGGAGAAGGAAGAAT+TGG | - | Chr5:36982832-36982851 | MsG0580026422.01.T01:CDS | 50.0% | |
| TACAGTGTCTCTTCTGGCCT+CGG | + | Chr5:36982716-36982735 | None:intergenic | 50.0% | |
| ! | TTGGTAAAGCGCCTCTTACG+CGG | + | Chr5:36982641-36982660 | None:intergenic | 50.0% |
| !! | TTGATTTTGGAGGCATGGCG+AGG | - | Chr5:36982935-36982954 | MsG0580026422.01.T01:CDS | 50.0% |
| CCGCTGCAGACTCATCAAAC+CGG | - | Chr5:36982615-36982634 | MsG0580026422.01.T01:CDS | 55.0% | |
| GTTTGATGAGTCTGCAGCGG+CGG | + | Chr5:36982615-36982634 | None:intergenic | 55.0% | |
| ! | CCGGTTTGATGAGTCTGCAG+CGG | + | Chr5:36982618-36982637 | None:intergenic | 55.0% |
| CATCAAACCGGCCGCGTAAG+AGG | - | Chr5:36982627-36982646 | MsG0580026422.01.T01:CDS | 60.0% | |
| ! | TAAAGCGCCTCTTACGCGGC+CGG | + | Chr5:36982637-36982656 | None:intergenic | 60.0% |
| TGATGAGTCTGCAGCGGCGG+CGG | + | Chr5:36982612-36982631 | None:intergenic | 65.0% | |
| TGAGTCTGCAGCGGCGGCGG+CGG | + | Chr5:36982609-36982628 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 36982587 | 36982967 | 36982587 | ID=MsG0580026422.01;Name=MsG0580026422.01 |
| Chr5 | mRNA | 36982587 | 36982967 | 36982587 | ID=MsG0580026422.01.T01;Parent=MsG0580026422.01;Name=MsG0580026422.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|126 |
| Chr5 | exon | 36982587 | 36982967 | 36982587 | ID=MsG0580026422.01.T01:exon:32136;Parent=MsG0580026422.01.T01 |
| Chr5 | CDS | 36982587 | 36982967 | 36982587 | ID=MsG0580026422.01.T01:cds;Parent=MsG0580026422.01.T01 |
| Gene Sequence |
| Protein sequence |
