Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028491.01.T01 | AFK39241.1 | 84.524 | 84 | 13 | 0 | 6 | 89 | 173 | 256 | 2.92E-39 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028491.01.T01 | O64732 | 49.398 | 83 | 40 | 1 | 6 | 88 | 357 | 437 | 1.82E-17 | 78.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028491.01.T01 | I3SG49 | 84.524 | 84 | 13 | 0 | 6 | 89 | 173 | 256 | 1.39e-39 | 140 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003849.01 | MsG0580028491.01 | 0.800144 | 1.653764e-48 | 7.643231e-46 |
| MsG0580024218.01 | MsG0580028491.01 | 0.809468 | 1.864679e-50 | 1.095664e-47 |
| MsG0580025682.01 | MsG0580028491.01 | 0.826486 | 2.642154e-54 | 2.481147e-51 |
| MsG0580026162.01 | MsG0580028491.01 | 0.804417 | 2.181566e-49 | 1.124191e-46 |
| MsG0580028490.01 | MsG0580028491.01 | 0.845366 | 4.246335e-59 | 7.059857e-56 |
| MsG0580028491.01 | MsG0780039576.01 | 0.807891 | 4.050252e-50 | 2.282592e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028491.01.T01 | MTR_5g072840 | 84.524 | 84 | 13 | 0 | 6 | 89 | 367 | 450 | 2.52e-42 | 143 |
| MsG0580028491.01.T01 | MTR_5g072820 | 84.524 | 84 | 13 | 0 | 6 | 89 | 367 | 450 | 2.80e-42 | 143 |
| MsG0580028491.01.T01 | MTR_5g072830 | 84.524 | 84 | 13 | 0 | 6 | 89 | 367 | 450 | 1.55e-41 | 141 |
| MsG0580028491.01.T01 | MTR_5g072810 | 83.333 | 84 | 14 | 0 | 6 | 89 | 372 | 455 | 2.22e-39 | 135 |
| MsG0580028491.01.T01 | MTR_5g072827 | 84.615 | 52 | 8 | 0 | 37 | 88 | 35 | 86 | 4.03e-24 | 87.8 |
| MsG0580028491.01.T01 | MTR_5g072860 | 49.425 | 87 | 42 | 1 | 1 | 87 | 381 | 465 | 7.77e-20 | 82.4 |
| MsG0580028491.01.T01 | MTR_3g031400 | 44.444 | 81 | 43 | 1 | 7 | 87 | 371 | 449 | 4.71e-18 | 77.4 |
| MsG0580028491.01.T01 | MTR_3g031450 | 41.975 | 81 | 45 | 1 | 7 | 87 | 373 | 451 | 3.95e-16 | 72.0 |
| MsG0580028491.01.T01 | MTR_5g075440 | 39.759 | 83 | 48 | 1 | 5 | 87 | 375 | 455 | 1.01e-15 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028491.01.T01 | AT2G30150 | 49.398 | 83 | 40 | 1 | 6 | 88 | 373 | 453 | 2.30e-20 | 84.0 |
| MsG0580028491.01.T01 | AT2G30150 | 49.398 | 83 | 40 | 1 | 6 | 88 | 357 | 437 | 1.85e-18 | 78.6 |
| MsG0580028491.01.T01 | AT2G30140 | 42.857 | 84 | 46 | 1 | 6 | 89 | 371 | 452 | 2.58e-17 | 75.5 |
| MsG0580028491.01.T01 | AT2G30140 | 42.857 | 84 | 46 | 1 | 6 | 89 | 372 | 453 | 2.74e-17 | 75.1 |
| MsG0580028491.01.T01 | AT2G31750 | 39.024 | 82 | 46 | 1 | 6 | 87 | 377 | 454 | 7.99e-12 | 59.7 |
| MsG0580028491.01.T01 | AT2G31750 | 39.024 | 82 | 46 | 1 | 6 | 87 | 377 | 454 | 8.78e-12 | 59.7 |
Find 15 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGCTTGTTAAGAGGTTTA+TGG | 0.222847 | 5:+81383513 | MsG0580028491.01.T01:CDS |
| AGCTCTACTATATATTCTTT+TGG | 0.224303 | 5:-81383496 | None:intergenic |
| AGATAGAGCAAGAGAACTTA+AGG | 0.368648 | 5:+81383567 | MsG0580028491.01.T01:CDS |
| TCAGTGACATTTCGTCTTGA+AGG | 0.377463 | 5:+81383650 | MsG0580028491.01.T01:CDS |
| GTACTAAAATTGCTGATGAA+TGG | 0.437074 | 5:+81383419 | MsG0580028491.01.T01:CDS |
| ATATAGTAGAGCTTGTTAAG+AGG | 0.445793 | 5:+81383506 | MsG0580028491.01.T01:CDS |
| TGTCATAAAGCTATTGCTAA+AGG | 0.496050 | 5:+81383595 | MsG0580028491.01.T01:CDS |
| CTAAAGGCGGATCCTCAGAT+AGG | 0.505408 | 5:+81383611 | MsG0580028491.01.T01:CDS |
| AAGGTAGAGAAATGGGTGGA+AGG | 0.592368 | 5:+81383389 | None:intergenic |
| ATGGATCTTGAAAGCCAAGA+GGG | 0.593643 | 5:+81383532 | MsG0580028491.01.T01:CDS |
| TGCGTCGAGGTTCCTATCTG+AGG | 0.618959 | 5:-81383623 | None:intergenic |
| CATAAAGCTATTGCTAAAGG+CGG | 0.654547 | 5:+81383598 | MsG0580028491.01.T01:CDS |
| GTGGAAGGTAGAGAAATGGG+TGG | 0.698734 | 5:+81383385 | None:intergenic |
| TATGGATCTTGAAAGCCAAG+AGG | 0.700003 | 5:+81383531 | MsG0580028491.01.T01:CDS |
| TGTCACTGATAAATGCGTCG+AGG | 0.739450 | 5:-81383636 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGCTCTACTATATATTCTTT+TGG | - | Chr5:81383499-81383518 | None:intergenic | 25.0% |
| ATTGCTGATGAATGGAAAAA+TGG | + | Chr5:81383427-81383446 | MsG0580028491.01.T01:CDS | 30.0% | |
| GTACTAAAATTGCTGATGAA+TGG | + | Chr5:81383419-81383438 | MsG0580028491.01.T01:CDS | 30.0% | |
| TGTCATAAAGCTATTGCTAA+AGG | + | Chr5:81383595-81383614 | MsG0580028491.01.T01:CDS | 30.0% | |
| TTGCTGATGAATGGAAAAAT+GGG | + | Chr5:81383428-81383447 | MsG0580028491.01.T01:CDS | 30.0% | |
| !! | ATATAGTAGAGCTTGTTAAG+AGG | + | Chr5:81383506-81383525 | MsG0580028491.01.T01:CDS | 30.0% |
| AGATAGAGCAAGAGAACTTA+AGG | + | Chr5:81383567-81383586 | MsG0580028491.01.T01:CDS | 35.0% | |
| CATAAAGCTATTGCTAAAGG+CGG | + | Chr5:81383598-81383617 | MsG0580028491.01.T01:CDS | 35.0% | |
| ! | CTCTAATTTTCTTTCCCTCT+TGG | - | Chr5:81383549-81383568 | None:intergenic | 35.0% |
| !! | AGAGCTTGTTAAGAGGTTTA+TGG | + | Chr5:81383513-81383532 | MsG0580028491.01.T01:CDS | 35.0% |
| ATGGATCTTGAAAGCCAAGA+GGG | + | Chr5:81383532-81383551 | MsG0580028491.01.T01:CDS | 40.0% | |
| CTGATGAATGGAAAAATGGG+TGG | + | Chr5:81383431-81383450 | MsG0580028491.01.T01:CDS | 40.0% | |
| TATGGATCTTGAAAGCCAAG+AGG | + | Chr5:81383531-81383550 | MsG0580028491.01.T01:CDS | 40.0% | |
| TGAATGGAAAAATGGGTGGA+AGG | + | Chr5:81383435-81383454 | MsG0580028491.01.T01:CDS | 40.0% | |
| TGTCACTGATAAATGCGTCG+AGG | - | Chr5:81383639-81383658 | None:intergenic | 45.0% | |
| CTAAAGGCGGATCCTCAGAT+AGG | + | Chr5:81383611-81383630 | MsG0580028491.01.T01:CDS | 50.0% | |
| TGCGTCGAGGTTCCTATCTG+AGG | - | Chr5:81383626-81383645 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 81383400 | 81383669 | 81383400 | ID=MsG0580028491.01;Name=MsG0580028491.01 |
| Chr5 | mRNA | 81383400 | 81383669 | 81383400 | ID=MsG0580028491.01.T01;Parent=MsG0580028491.01;Name=MsG0580028491.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr5 | exon | 81383400 | 81383669 | 81383400 | ID=MsG0580028491.01.T01:exon:29557;Parent=MsG0580028491.01.T01 |
| Chr5 | CDS | 81383400 | 81383669 | 81383400 | ID=MsG0580028491.01.T01:cds;Parent=MsG0580028491.01.T01 |
| Gene Sequence |
| Protein sequence |
