AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028616.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028616.01.T01 MTR_5g075690 98.571 70 1 0 1 70 1 70 4.54e-46 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028616.01.T01 AT5G05370 77.778 72 16 0 1 72 1 72 6.26e-37 118
MsG0580028616.01.T01 AT3G10860 77.778 72 16 0 1 72 1 72 6.21e-36 115
MsG0580028616.01.T01 AT3G10860 77.778 72 16 0 1 72 1 72 6.21e-36 115

Find 17 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACAAAGTCTCCGAAAAT+TGG 0.241137 5:-83475094 MsG0580028616.01.T01:CDS
GGAGACTTTGTGATGAATTT+TGG 0.245276 5:+83475103 None:intergenic
TGCTATGCACTTCGTGGAAA+AGG 0.420287 5:-83471623 MsG0580028616.01.T01:CDS
TACGAGTAGGTGCCGACGAC+GGG 0.436149 5:+83475047 None:intergenic
GGGAAGATCCTTCCACAAAC+CGG 0.437119 5:+83475127 None:intergenic
TTGGATCAGCGCTCTTCTCT+TGG 0.444848 5:-83475075 MsG0580028616.01.T01:CDS
CTCTTGGTTGTTCCCGTCGT+CGG 0.487685 5:-83475059 MsG0580028616.01.T01:CDS
AGAAAATCATGCCCGGTTTG+TGG 0.493137 5:-83475139 MsG0580028616.01.T01:CDS
CAGGTATGCTATGCACTTCG+TGG 0.513534 5:-83471629 MsG0580028616.01.T01:CDS
GGAAGATCCTTCCACAAACC+GGG 0.541745 5:+83475128 None:intergenic
TTCCAGCAGAAAATCATGCC+CGG 0.563414 5:-83475146 MsG0580028616.01.T01:CDS
AAAAGGAGAAGCTGCACCAC+AGG 0.578417 5:-83471606 MsG0580028616.01.T01:CDS
AATCATGCCCGGTTTGTGGA+AGG 0.588851 5:-83475135 MsG0580028616.01.T01:CDS
GCATACACAACGGATTTCAC+AGG 0.612168 5:+83475179 None:intergenic
TCGCATGAGCTCAGTACCTG+TGG 0.638105 5:+83471590 None:intergenic
TTACGAGTAGGTGCCGACGA+CGG 0.648744 5:+83475046 None:intergenic
AGGAGAAAGAGCATACACAA+CGG 0.765552 5:+83475169 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGATTTCATTTTTAAATTA+CGG - Chr5:83472476-83472495 MsG0580028616.01.T01:intron 10.0%
!!! TGTTTTTCTTTTTTAAATTA+CGG + Chr5:83472605-83472624 None:intergenic 10.0%
!!! TTTGTGTTTATATTTTTTTT+AGG - Chr5:83472768-83472787 MsG0580028616.01.T01:intron 10.0%
!!! TTTTGCTAAATAATTTTTTT+TGG + Chr5:83473346-83473365 None:intergenic 10.0%
!! AATAATAATAAAATAGAGAG+AGG + Chr5:83471780-83471799 None:intergenic 15.0%
!! AGAAAGTTAATAATTCATTA+TGG - Chr5:83473464-83473483 MsG0580028616.01.T01:intron 15.0%
!! ATAATAATAAAATAGAGAGA+GGG + Chr5:83471779-83471798 None:intergenic 15.0%
!! ATATATAACGGTTAAATAAA+GGG - Chr5:83474611-83474630 MsG0580028616.01.T01:intron 15.0%
!! GAAAGTTAATAATTCATTAT+GGG - Chr5:83473465-83473484 MsG0580028616.01.T01:intron 15.0%
!! TATATAAATAAACACATTGA+AGG + Chr5:83474304-83474323 None:intergenic 15.0%
!! TTTGTTTATAATTGAGTTTA+AGG - Chr5:83472963-83472982 MsG0580028616.01.T01:intron 15.0%
!!! ATCTTACTTATTTTTTAACT+AGG - Chr5:83471844-83471863 MsG0580028616.01.T01:intron 15.0%
!!! TCTTACTTATTTTTTAACTA+GGG - Chr5:83471845-83471864 MsG0580028616.01.T01:intron 15.0%
!!! TTTTGTATAGTGATATATAA+CGG - Chr5:83474599-83474618 MsG0580028616.01.T01:intron 15.0%
!!! TTTTTTTAATTGTGTTTACA+AGG - Chr5:83474962-83474981 MsG0580028616.01.T01:intron 15.0%
!! AAATAGATCCAAATATTAAG+TGG - Chr5:83474543-83474562 MsG0580028616.01.T01:intron 20.0%
!! ATACTTGAAAAATAAGTTTG+AGG - Chr5:83473925-83473944 MsG0580028616.01.T01:intron 20.0%
!! ATCAACAACATATTTACTAA+GGG - Chr5:83472409-83472428 MsG0580028616.01.T01:intron 20.0%
!! ATTTGCAAAAAAAAACATAC+TGG + Chr5:83473709-83473728 None:intergenic 20.0%
!! GATATATAACGGTTAAATAA+AGG - Chr5:83474610-83474629 MsG0580028616.01.T01:intron 20.0%
!! GTGAATATTATGTTGTAAAT+AGG + Chr5:83474393-83474412 None:intergenic 20.0%
!!! AAATTGATAGCTTTATTGTT+AGG + Chr5:83472023-83472042 None:intergenic 20.0%
!!! AATAAAGCTATCAATTTTAG+TGG - Chr5:83472026-83472045 MsG0580028616.01.T01:intron 20.0%
!!! ACTTTGTGTAATATTTTGAA+TGG - Chr5:83474207-83474226 MsG0580028616.01.T01:intron 20.0%
!!! ATAATTTTGTTGATTGAGTT+TGG - Chr5:83471890-83471909 MsG0580028616.01.T01:intron 20.0%
!!! ATTGTTAGGTTGAAAATTTT+AGG + Chr5:83472009-83472028 None:intergenic 20.0%
!!! CTTAATATTTGGATCTATTT+TGG + Chr5:83474543-83474562 None:intergenic 20.0%
!!! TTTTTTTGCAAATTTTTTGC+CGG - Chr5:83473716-83473735 MsG0580028616.01.T01:intron 20.0%
! AAATAAAGGGACAAAATTGT+GGG - Chr5:83474624-83474643 MsG0580028616.01.T01:intron 25.0%
! AAGATAAATTCAGAAAACAG+AGG + Chr5:83474242-83474261 None:intergenic 25.0%
! AATTGGGAAAAGGATATATT+TGG - Chr5:83474911-83474930 MsG0580028616.01.T01:intron 25.0%
! AGATAAATTCAGAAAACAGA+GGG + Chr5:83474241-83474260 None:intergenic 25.0%
! AGATGTATGGAATGTTAATT+GGG - Chr5:83474895-83474914 MsG0580028616.01.T01:intron 25.0%
! AGGCAAAAAAAAATTGTCAT+TGG + Chr5:83472734-83472753 None:intergenic 25.0%
! ATACACTTGTGATAATAGAA+GGG - Chr5:83474667-83474686 MsG0580028616.01.T01:intron 25.0%
! ATACATAATACAATGCTAAC+AGG + Chr5:83475071-83475090 None:intergenic 25.0%
! ATGAGATATTAAACATTCAC+AGG + Chr5:83475141-83475160 None:intergenic 25.0%
! CATCAACAACATATTTACTA+AGG - Chr5:83472408-83472427 MsG0580028616.01.T01:intron 25.0%
! GTCTTCATAAAATAAAACCT+GGG - Chr5:83473679-83473698 MsG0580028616.01.T01:intron 25.0%
! TAAATAAAGGGACAAAATTG+TGG - Chr5:83474623-83474642 MsG0580028616.01.T01:intron 25.0%
! TACATAATACAATGCTAACA+GGG + Chr5:83475070-83475089 None:intergenic 25.0%
! TAGATGTATGGAATGTTAAT+TGG - Chr5:83474894-83474913 MsG0580028616.01.T01:intron 25.0%
! TAGCATTGTATTATGTATCT+GGG - Chr5:83475073-83475092 MsG0580028616.01.T01:CDS 25.0%
! TATACACTTGTGATAATAGA+AGG - Chr5:83474666-83474685 MsG0580028616.01.T01:intron 25.0%
! TATCACTATACAAAAGTCAA+AGG + Chr5:83474594-83474613 None:intergenic 25.0%
! TATTATGTTGTAAATAGGCT+TGG + Chr5:83474424-83474443 None:intergenic 25.0%
! TCAATAAGAATATGACAAGA+TGG - Chr5:83472368-83472387 MsG0580028616.01.T01:intron 25.0%
! TTAATGTCATCTAATATTGC+TGG - Chr5:83472677-83472696 MsG0580028616.01.T01:intron 25.0%
! TTAGCATTGTATTATGTATC+TGG - Chr5:83475072-83475091 MsG0580028616.01.T01:CDS 25.0%
!! AGTAAATATGTTGTTGATGA+AGG + Chr5:83472407-83472426 None:intergenic 25.0%
!! ATGACATTAACAAAGCATTA+GGG + Chr5:83472667-83472686 None:intergenic 25.0%
!! GTAAATATGTTGTTGATGAA+GGG + Chr5:83472406-83472425 None:intergenic 25.0%
!! TAACTTTCTGAGCAATATTT+TGG + Chr5:83473453-83473472 None:intergenic 25.0%
!! TAATTGTTAGCTTACAATCT+TGG + Chr5:83474005-83474024 None:intergenic 25.0%
!! TTGGTATCGAATATTGAAAT+GGG - Chr5:83474178-83474197 MsG0580028616.01.T01:intron 25.0%
!! TTTGGTATCGAATATTGAAA+TGG - Chr5:83474177-83474196 MsG0580028616.01.T01:intron 25.0%
!! TTTTCCAAAAATATTCTCGA+TGG - Chr5:83472285-83472304 MsG0580028616.01.T01:intron 25.0%
!!! AATATTTGGATCTATTTTGG+AGG + Chr5:83474540-83474559 None:intergenic 25.0%
!!! AGCTTTTAAAAACAAGATTG+TGG - Chr5:83471973-83471992 MsG0580028616.01.T01:intron 25.0%
!!! ATATTTGGATCTATTTTGGA+GGG + Chr5:83474539-83474558 None:intergenic 25.0%
!!! ATTTTAGTGGAACTAAGATT+TGG - Chr5:83472039-83472058 MsG0580028616.01.T01:intron 25.0%
!!! TGCAGTGTCTTTATTTTTAA+CGG - Chr5:83475032-83475051 MsG0580028616.01.T01:intron 25.0%
!!! TTGCTTGTTATGATTCTATT+TGG - Chr5:83472885-83472904 MsG0580028616.01.T01:intron 25.0%
!!! TTTTTTAGGATTGAATTGCT+AGG - Chr5:83472782-83472801 MsG0580028616.01.T01:intron 25.0%
!!! TTTTTTTCCTTCTTAATACC+AGG - Chr5:83474743-83474762 MsG0580028616.01.T01:intron 25.0%
AAAAGCCTCCACTTAATATT+TGG + Chr5:83474554-83474573 None:intergenic 30.0%
AACAAGATTGTGGTAAAACT+TGG - Chr5:83471983-83472002 MsG0580028616.01.T01:intron 30.0%
AATATTCGATACCAAAACAG+AGG + Chr5:83474173-83474192 None:intergenic 30.0%
ACACATAATGTTCACAGTTT+AGG + Chr5:83474997-83475016 None:intergenic 30.0%
ATATTCGATACCAAAACAGA+GGG + Chr5:83474172-83474191 None:intergenic 30.0%
ATGGAATGTTAATTGGGAAA+AGG - Chr5:83474901-83474920 MsG0580028616.01.T01:intron 30.0%
ATTGAGTTTAAGGATGTGTA+CGG - Chr5:83472973-83472992 MsG0580028616.01.T01:intron 30.0%
ATTTCTGCTAATACCTAAAG+AGG + Chr5:83474141-83474160 None:intergenic 30.0%
CAAAAATATTCTCGATGGAT+GGG - Chr5:83472290-83472309 MsG0580028616.01.T01:intron 30.0%
CACATAATGTTCACAGTTTA+GGG + Chr5:83474996-83475015 None:intergenic 30.0%
CGTCTTCATAAAATAAAACC+TGG - Chr5:83473678-83473697 MsG0580028616.01.T01:intron 30.0%
CTTATTTGGTATCAGAGTAT+CGG - Chr5:83474841-83474860 MsG0580028616.01.T01:intron 30.0%
CTTCTTAATACCAGGAATAT+GGG - Chr5:83474751-83474770 MsG0580028616.01.T01:intron 30.0%
GTGTAGAGTCAAAACTTATT+TGG - Chr5:83474827-83474846 MsG0580028616.01.T01:intron 30.0%
TACACTTGTGATAATAGAAG+GGG - Chr5:83474668-83474687 MsG0580028616.01.T01:intron 30.0%
TAGATCCAAATATTAAGTGG+AGG - Chr5:83474546-83474565 MsG0580028616.01.T01:intron 30.0%
TAGATCTAAATCTCAAGTGA+TGG + Chr5:83473647-83473666 None:intergenic 30.0%
TGTCTCAACATACTTGAATT+TGG - Chr5:83474261-83474280 MsG0580028616.01.T01:intron 30.0%
TTAATATCTCATACGAATGC+AGG - Chr5:83475149-83475168 MsG0580028616.01.T01:CDS 30.0%
TTTGGATTGAGAGTTTATGA+GGG + Chr5:83472640-83472659 None:intergenic 30.0%
! AAACAATGAGGCTTTGTATT+AGG + Chr5:83473975-83473994 None:intergenic 30.0%
! AAGGATATATTTGGAGTACA+AGG - Chr5:83474920-83474939 MsG0580028616.01.T01:intron 30.0%
! ACCATAATCAAATCAGCTTT+TGG - Chr5:83474362-83474381 MsG0580028616.01.T01:intron 30.0%
! AGGATATATTTGGAGTACAA+GGG - Chr5:83474921-83474940 MsG0580028616.01.T01:intron 30.0%
! ATATTTACTAAGGGTGTGTT+TGG - Chr5:83472418-83472437 MsG0580028616.01.T01:intron 30.0%
! GATGACATTAACAAAGCATT+AGG + Chr5:83472668-83472687 None:intergenic 30.0%
! GTTAGAGTTTAGAGGTTTTT+GGG + Chr5:83473625-83473644 None:intergenic 30.0%
! TAGTTTGGTATGCTTTTCAA+TGG - Chr5:83474107-83474126 MsG0580028616.01.T01:intron 30.0%
! TTTTTGTGGTCAACTTTTGT+TGG - Chr5:83472912-83472931 MsG0580028616.01.T01:intron 30.0%
!! AATGGATTAGTATCCTCTTT+AGG - Chr5:83474125-83474144 MsG0580028616.01.T01:intron 30.0%
!! ACCAAAAGCTGATTTGATTA+TGG + Chr5:83474366-83474385 None:intergenic 30.0%
!! ACTTTGTGATGAATTTTGGT+GGG + Chr5:83471693-83471712 None:intergenic 30.0%
!! AGACATGAACTTAGTTTGTT+GGG - Chr5:83472151-83472170 MsG0580028616.01.T01:intron 30.0%
!! ATGGGTTGAAGAAATTTTAC+AGG + Chr5:83473590-83473609 None:intergenic 30.0%
!! TAGACATGAACTTAGTTTGT+TGG - Chr5:83472150-83472169 MsG0580028616.01.T01:intron 30.0%
!! TATTTGGATCTATTTTGGAG+GGG + Chr5:83474538-83474557 None:intergenic 30.0%
!! TTTGCTTTTGCGATTTTGAA+CGG - Chr5:83471816-83471835 MsG0580028616.01.T01:intron 30.0%
!!! TGCTGGACATTTTTTACATT+GGG + Chr5:83473506-83473525 None:intergenic 30.0%
!!! TTCTATTTGGTGTGTTTTTG+TGG - Chr5:83472898-83472917 MsG0580028616.01.T01:intron 30.0%
!!! TTGCTGGACATTTTTTACAT+TGG + Chr5:83473507-83473526 None:intergenic 30.0%
AAAAACATACTGGTAAACCC+AGG + Chr5:83473699-83473718 None:intergenic 35.0%
AAATATCAATCTCTCGCAGT+TGG - Chr5:83475108-83475127 MsG0580028616.01.T01:CDS 35.0%
AAGTGATGGTTAGAGTTTAG+AGG + Chr5:83473633-83473652 None:intergenic 35.0%
AATGTGAAATGATTTCCTCC+AGG + Chr5:83474061-83474080 None:intergenic 35.0%
ACAAAATTGTGGGACATTAG+TGG - Chr5:83474634-83474653 MsG0580028616.01.T01:intron 35.0%
ACATAATTCGTAGTTCGCTT+CGG - Chr5:83473091-83473110 MsG0580028616.01.T01:intron 35.0%
ACCATAGTCACATAATTCGT+CGG + Chr5:83473851-83473870 None:intergenic 35.0%
ATACGAAAATCACGATTGTC+TGG - Chr5:83472095-83472114 MsG0580028616.01.T01:intron 35.0%
ATATTCAACTCCCATATTCC+TGG + Chr5:83474764-83474783 None:intergenic 35.0%
ATCACAAAGTCTCCGAAAAT+TGG - Chr5:83471703-83471722 MsG0580028616.01.T01:intron 35.0%
CATAATATTGAGTCAGCTCT+AGG - Chr5:83474448-83474467 MsG0580028616.01.T01:intron 35.0%
CCAAAAATATTCTCGATGGA+TGG - Chr5:83472289-83472308 MsG0580028616.01.T01:intron 35.0%
CCATATTCCTGGTATTAAGA+AGG + Chr5:83474753-83474772 None:intergenic 35.0%
CCTTCTTAATACCAGGAATA+TGG - Chr5:83474750-83474769 MsG0580028616.01.T01:intron 35.0%
CTTGGTTAGACTAAACAATG+AGG + Chr5:83473987-83474006 None:intergenic 35.0%
GAATCGAACAGTATTGTGAT+AGG - Chr5:83472836-83472855 MsG0580028616.01.T01:intron 35.0%
GATTGAATTGCTAGGAGAAA+GGG - Chr5:83472790-83472809 MsG0580028616.01.T01:intron 35.0%
GCATTCTGACATTAGATGTA+TGG - Chr5:83474882-83474901 MsG0580028616.01.T01:intron 35.0%
GTTCATGTCTAGATTTACAG+TGG + Chr5:83472141-83472160 None:intergenic 35.0%
GTTTGGATTGAGAGTTTATG+AGG + Chr5:83472641-83472660 None:intergenic 35.0%
TACCAAACTAAGTTCATGTC+TGG + Chr5:83474097-83474116 None:intergenic 35.0%
TAGAAGGGGATTTCTTGAAT+AGG - Chr5:83474682-83474701 MsG0580028616.01.T01:intron 35.0%
TATGGGAGTTGAATATGACA+TGG - Chr5:83474768-83474787 MsG0580028616.01.T01:intron 35.0%
TGTAAAAAATGTCCAGCAAC+TGG - Chr5:83473508-83473527 MsG0580028616.01.T01:intron 35.0%
TTGGATTGAGAGTTTATGAG+GGG + Chr5:83472639-83472658 None:intergenic 35.0%
! CCATCCATCGAGAATATTTT+TGG + Chr5:83472292-83472311 None:intergenic 35.0%
! CTGCGAGAGATTGATATTTT+TGG + Chr5:83475107-83475126 None:intergenic 35.0%
! GACTTTGTGATGAATTTTGG+TGG + Chr5:83471694-83471713 None:intergenic 35.0%
! GGAGACTTTGTGATGAATTT+TGG + Chr5:83471697-83471716 None:intergenic 35.0%
! GGTTAGAGTTTAGAGGTTTT+TGG + Chr5:83473626-83473645 None:intergenic 35.0%
! GGTTGAAGAAATTTTACAGG+AGG + Chr5:83473587-83473606 None:intergenic 35.0%
! TGAAGAAATTTTACAGGAGG+AGG + Chr5:83473584-83473603 None:intergenic 35.0%
! TGACAAGATGGTTTTCTGAA+TGG - Chr5:83472380-83472399 MsG0580028616.01.T01:intron 35.0%
! TTGTTGATTGAGTTTGGTTG+TGG - Chr5:83471896-83471915 MsG0580028616.01.T01:intron 35.0%
! TTTTGCGATTTTGAACGGTT+TGG - Chr5:83471821-83471840 MsG0580028616.01.T01:intron 35.0%
!! ATTTGGATCTATTTTGGAGG+GGG + Chr5:83474537-83474556 None:intergenic 35.0%
!! TTTGGGAAAGTGCTGAAAAT+GGG + Chr5:83473608-83473627 None:intergenic 35.0%
!!! ACATTTTTTACATTGGGCCT+GGG + Chr5:83473500-83473519 None:intergenic 35.0%
!!! GATTGATATTTTTGGCTACG+TGG + Chr5:83475099-83475118 None:intergenic 35.0%
!!! TTTTGGGAAAGTGCTGAAAA+TGG + Chr5:83473609-83473628 None:intergenic 35.0%
ACAACCAAATCCCTTGGTTA+GGG - Chr5:83474334-83474353 MsG0580028616.01.T01:intron 40.0%
AGGAACTCATTTCGTATGAC+AGG + Chr5:83474041-83474060 None:intergenic 40.0%
AGGAGGAAGATGTTGATATC+AGG + Chr5:83473538-83473557 None:intergenic 40.0%
AGTACTAGTACTACGAACCA+AGG + Chr5:83473195-83473214 None:intergenic 40.0%
ATCTAATGTCAGAATGCCAG+AGG + Chr5:83474879-83474898 None:intergenic 40.0%
ATGAGTACAACCAAATCCCT+TGG - Chr5:83474328-83474347 MsG0580028616.01.T01:intron 40.0%
ATTGAGAGTTTATGAGGGGA+GGG + Chr5:83472635-83472654 None:intergenic 40.0%
CGAATTATGTGACTATGGTC+TGG - Chr5:83473852-83473871 MsG0580028616.01.T01:intron 40.0%
GCAATCACATGTATAAGTGC+CGG + Chr5:83473002-83473021 None:intergenic 40.0%
GGAGGAAGATGTTGATATCA+GGG + Chr5:83473537-83473556 None:intergenic 40.0%
GGATTGAATTGCTAGGAGAA+AGG - Chr5:83472789-83472808 MsG0580028616.01.T01:intron 40.0%
GTCCAGACATGAACTTAGTT+TGG - Chr5:83474092-83474111 MsG0580028616.01.T01:intron 40.0%
GTGAATGCTATGTCGTCAAT+AGG + Chr5:83474479-83474498 None:intergenic 40.0%
TACAACCAAATCCCTTGGTT+AGG - Chr5:83474333-83474352 MsG0580028616.01.T01:intron 40.0%
TACGCGACATGAGAAGAATT+CGG - Chr5:83473140-83473159 MsG0580028616.01.T01:intron 40.0%
TCACAGCTTCAAGATGAAGT+AGG + Chr5:83472254-83472273 None:intergenic 40.0%
TCATGTCTGTTCTCTATGTC+TGG - Chr5:83473059-83473078 MsG0580028616.01.T01:intron 40.0%
TCCGACGAATTATGTGACTA+TGG - Chr5:83473847-83473866 MsG0580028616.01.T01:intron 40.0%
TGTCATACGAAATGAGTTCC+TGG - Chr5:83474040-83474059 MsG0580028616.01.T01:intron 40.0%
TTGACGACATAGCATTCACA+TGG - Chr5:83474480-83474499 MsG0580028616.01.T01:intron 40.0%
! ACAAAGCATTAGGGTGTGTT+TGG + Chr5:83472658-83472677 None:intergenic 40.0%
! AGAAATTTTACAGGAGGAGG+AGG + Chr5:83473581-83473600 None:intergenic 40.0%
! AGGAGAAAGAGCATACACAA+CGG + Chr5:83471631-83471650 None:intergenic 40.0%
! ATTCTTCGTTCCCTCTGTTT+TGG - Chr5:83474159-83474178 MsG0580028616.01.T01:intron 40.0%
! TTACTAAGGGTGTGTTTGGA+TGG - Chr5:83472422-83472441 MsG0580028616.01.T01:intron 40.0%
!! TAGTTTGTTGGGCTTCTCAA+TGG - Chr5:83472162-83472181 MsG0580028616.01.T01:intron 40.0%
!!! AAATTTTTTGCCGGTTCCTG+TGG - Chr5:83473725-83473744 MsG0580028616.01.T01:intron 40.0%
!!! AAGAGCGCTGATCCAATTTT+CGG + Chr5:83471718-83471737 None:intergenic 40.0%
!!! GACATTTTTTACATTGGGCC+TGG + Chr5:83473501-83473520 None:intergenic 40.0%
ACATGGCGAGCAATAGCATT+AGG - Chr5:83474785-83474804 MsG0580028616.01.T01:intron 45.0%
AGAAGAATTCGGTACGTCTG+GGG - Chr5:83473151-83473170 MsG0580028616.01.T01:intron 45.0%
AGAGAGAGGGAATTACGAGT+AGG + Chr5:83471766-83471785 None:intergenic 45.0%
ATATCAGGGTTTCCAGTTGC+TGG + Chr5:83473523-83473542 None:intergenic 45.0%
ATTATGGGCCAAACAAACCC+AGG - Chr5:83473480-83473499 MsG0580028616.01.T01:intron 45.0%
CAACCAAATCCCTTGGTTAG+GGG - Chr5:83474335-83474354 MsG0580028616.01.T01:intron 45.0%
CACAAAGCACAACAGAACAG+AGG + Chr5:83472754-83472773 None:intergenic 45.0%
CACATAATTCGTCGGAACAG+TGG + Chr5:83473843-83473862 None:intergenic 45.0%
CATACGAAATGAGTTCCTGG+AGG - Chr5:83474043-83474062 MsG0580028616.01.T01:intron 45.0%
CTGCTCAATTGAGTGAAAGG+AGG + Chr5:83473555-83473574 None:intergenic 45.0%
GAATGCCAGAGGACATCATT+AGG + Chr5:83474868-83474887 None:intergenic 45.0%
GAGAAGAATTCGGTACGTCT+GGG - Chr5:83473150-83473169 MsG0580028616.01.T01:intron 45.0%
GATTGAGAGTTTATGAGGGG+AGG + Chr5:83472636-83472655 None:intergenic 45.0%
GCGAATTCGTCAACGAATTC+AGG - Chr5:83473761-83473780 MsG0580028616.01.T01:intron 45.0%
TATCAATCTCTCGCAGTTGG+TGG - Chr5:83475111-83475130 MsG0580028616.01.T01:CDS 45.0%
TGAGAAGAATTCGGTACGTC+TGG - Chr5:83473149-83473168 MsG0580028616.01.T01:intron 45.0%
TGCTATGCACTTCGTGGAAA+AGG - Chr5:83475174-83475193 MsG0580028616.01.T01:CDS 45.0%
TGTGTTTGGATGGAGGGTTT+AGG - Chr5:83472432-83472451 MsG0580028616.01.T01:intron 45.0%
TGTTGGGCTTCTCAATGGAT+TGG - Chr5:83472167-83472186 MsG0580028616.01.T01:intron 45.0%
TTAAGGATGTGTACGGTGTC+CGG - Chr5:83472980-83472999 MsG0580028616.01.T01:intron 45.0%
TTCCAGCAGAAAATCATGCC+CGG - Chr5:83471651-83471670 MsG0580028616.01.T01:intron 45.0%
TTGAGAGTTTATGAGGGGAG+GGG + Chr5:83472634-83472653 None:intergenic 45.0%
TTTGGATGGAGGGTTTAGGA+GGG - Chr5:83472436-83472455 MsG0580028616.01.T01:intron 45.0%
! AATTTTACAGGAGGAGGAGG+CGG + Chr5:83473578-83473597 None:intergenic 45.0%
! AGAAAATCATGCCCGGTTTG+TGG - Chr5:83471658-83471677 MsG0580028616.01.T01:intron 45.0%
! GCATACACAACGGATTTCAC+AGG + Chr5:83471621-83471640 None:intergenic 45.0%
! GGCATGATTTTCTGCTGGAA+AGG + Chr5:83471651-83471670 None:intergenic 45.0%
! TAAGGGTGTGTTTGGATGGA+GGG - Chr5:83472426-83472445 MsG0580028616.01.T01:intron 45.0%
!! TTATTAAAAAAATTATTGAT+AGG + Chr5:83473237-83473256 None:intergenic 5.0%
AAAAGGAGAAGCTGCACCAC+AGG - Chr5:83475191-83475210 MsG0580028616.01.T01:CDS 50.0%
AACAAGCGTACCACAGGAAC+CGG + Chr5:83473738-83473757 None:intergenic 50.0%
AACAGTGGAGAACCGCGAAT+TGG + Chr5:83473828-83473847 None:intergenic 50.0%
CAGGTATGCTATGCACTTCG+TGG - Chr5:83475168-83475187 MsG0580028616.01.T01:CDS 50.0%
CGGCTGCTCAATTGAGTGAA+AGG + Chr5:83473558-83473577 None:intergenic 50.0%
GAAGAATTCGGTACGTCTGG+GGG - Chr5:83473152-83473171 MsG0580028616.01.T01:intron 50.0%
GGAAGATCCTTCCACAAACC+GGG + Chr5:83471672-83471691 None:intergenic 50.0%
GGGAAGATCCTTCCACAAAC+CGG + Chr5:83471673-83471692 None:intergenic 50.0%
GTTCATGTCTGGACGCACAA+TGG + Chr5:83474086-83474105 None:intergenic 50.0%
GTTTGGATGGAGGGTTTAGG+AGG - Chr5:83472435-83472454 MsG0580028616.01.T01:intron 50.0%
TATAGAGCTTCGGTACGCCT+TGG - Chr5:83473175-83473194 MsG0580028616.01.T01:intron 50.0%
TCGGACCTAATGATGTCCTC+TGG - Chr5:83474860-83474879 MsG0580028616.01.T01:intron 50.0%
TTCGCGGTTCTTTGGCGTAT+TGG + Chr5:83473791-83473810 None:intergenic 50.0%
TTGCCCCTAACCAAGGGATT+TGG + Chr5:83474341-83474360 None:intergenic 50.0%
TTGGATCAGCGCTCTTCTCT+TGG - Chr5:83471722-83471741 MsG0580028616.01.T01:intron 50.0%
TTGGATGGAGGGTTTAGGAG+GGG - Chr5:83472437-83472456 MsG0580028616.01.T01:intron 50.0%
! AACCGGGCATGATTTTCTGC+TGG + Chr5:83471656-83471675 None:intergenic 50.0%
! AATCATGCCCGGTTTGTGGA+AGG - Chr5:83471662-83471681 MsG0580028616.01.T01:intron 50.0%
! ACATTGGGCCTGGGTTTGTT+TGG + Chr5:83473491-83473510 None:intergenic 50.0%
! ATGGTCTTGCCCCTAACCAA+GGG + Chr5:83474347-83474366 None:intergenic 50.0%
! CTAAGGGTGTGTTTGGATGG+AGG - Chr5:83472425-83472444 MsG0580028616.01.T01:intron 50.0%
! TATGGTCTTGCCCCTAACCA+AGG + Chr5:83474348-83474367 None:intergenic 50.0%
AGAGTTTATGAGGGGAGGGG+AGG + Chr5:83472631-83472650 None:intergenic 55.0%
ATGGAGGGTTTAGGAGGGGA+AGG - Chr5:83472441-83472460 MsG0580028616.01.T01:intron 55.0%
CGCAGAAACAAGCGTACCAC+AGG + Chr5:83473744-83473763 None:intergenic 55.0%
CTCTTGGTTGTTCCCGTCGT+CGG - Chr5:83471738-83471757 MsG0580028616.01.T01:intron 55.0%
GAGTTTATGAGGGGAGGGGA+GGG + Chr5:83472630-83472649 None:intergenic 55.0%
GCGAATTGGTACGCGAGAAC+CGG + Chr5:83473814-83473833 None:intergenic 55.0%
GGTTCTCGCGTACCAATTCG+CGG - Chr5:83473813-83473832 MsG0580028616.01.T01:intron 55.0%
GTTCGCTTCGGTACGTGGTA+CGG - Chr5:83473103-83473122 MsG0580028616.01.T01:intron 55.0%
TACGCCAAAGAACCGCGAAC+CGG - Chr5:83473792-83473811 MsG0580028616.01.T01:intron 55.0%
TCGTAGTTCGCTTCGGTACG+TGG - Chr5:83473098-83473117 MsG0580028616.01.T01:intron 55.0%
TGGAGGGTTTAGGAGGGGAA+GGG - Chr5:83472442-83472461 MsG0580028616.01.T01:intron 55.0%
TTCGCTTCGGTACGTGGTAC+GGG - Chr5:83473104-83473123 MsG0580028616.01.T01:intron 55.0%
TTCGGTACGTGGTACGGGTT+CGG - Chr5:83473109-83473128 MsG0580028616.01.T01:intron 55.0%
! CGTCTGGGGGTATAGAGCTT+CGG - Chr5:83473165-83473184 MsG0580028616.01.T01:intron 55.0%
!! AGAACCGGTTCGCGGTTCTT+TGG + Chr5:83473799-83473818 None:intergenic 55.0%
!! GGTCTGGTGTTGTGCTTCAG+AGG - Chr5:83473868-83473887 MsG0580028616.01.T01:intron 55.0%
!! TTACGAGTAGGTGCCGACGA+CGG + Chr5:83471754-83471773 None:intergenic 55.0%
AGGGTTTAGGAGGGGAAGGG+AGG - Chr5:83472445-83472464 MsG0580028616.01.T01:intron 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG - Chr5:83472446-83472465 MsG0580028616.01.T01:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG - Chr5:83472447-83472466 MsG0580028616.01.T01:intron 60.0%
TCGGTACGTGGTACGGGTTC+GGG - Chr5:83473110-83473129 MsG0580028616.01.T01:intron 60.0%
!! TACGAGTAGGTGCCGACGAC+GGG + Chr5:83471753-83471772 None:intergenic 60.0%
GTGGTACGGGTTCGGGTACG+CGG - Chr5:83473117-83473136 MsG0580028616.01.T01:intron 65.0%
TAGGAGGGGAAGGGAGGGGA+GGG - Chr5:83472451-83472470 MsG0580028616.01.T01:intron 65.0%
TTAGGAGGGGAAGGGAGGGG+AGG - Chr5:83472450-83472469 MsG0580028616.01.T01:intron 65.0%
!! GGTACGCGAGAACCGGTTCG+CGG + Chr5:83473807-83473826 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 83471600 83475219 83471600 ID=MsG0580028616.01;Name=MsG0580028616.01
Chr5 mRNA 83471600 83475219 83471600 ID=MsG0580028616.01.T01;Parent=MsG0580028616.01;Name=MsG0580028616.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|0|0|2|0|72
Chr5 exon 83471600 83471648 83471600 ID=MsG0580028616.01.T01:exon:33448;Parent=MsG0580028616.01.T01
Chr5 exon 83475050 83475219 83475050 ID=MsG0580028616.01.T01:exon:33447;Parent=MsG0580028616.01.T01
Chr5 CDS 83475050 83475219 83475050 ID=MsG0580028616.01.T01:cds;Parent=MsG0580028616.01.T01
Chr5 CDS 83471600 83471648 83471600 ID=MsG0580028616.01.T01:cds;Parent=MsG0580028616.01.T01
Gene Sequence

>MsG0580028616.01.T01

ATGGGTAAAAACATCGTGCCTGTGAAATCCGTTGTGTATGCTCTTTCTCCTTTCCAGCAGAAAATCATGCCCGGTTTGTGGAAGGATCTTCCCACCAAAATTCATCACAAAGTCTCCGAAAATTGGATCAGCGCTCTTCTCTTGGTTGTTCCCGTCGTCGGCACCTACTCGTATGCTATGCACTTCGTGGAAAAGGAGAAGCTGCACCACAGGTACTGA
Protein sequence

>MsG0580028616.01.T01

MGKNIVPVKSVVYALSPFQQKIMPGLWKDLPTKIHHKVSENWISALLLVVPVVGTYSYAMHFVEKEKLHHRY*