Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023925.01.T01 | XP_003630917.1 | 100 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 3.45E-89 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001169.01 | MsG0480023925.01 | 0.817762 | 2.787659e-52 | 2.048264e-49 |
| MsG0180001837.01 | MsG0480023925.01 | 0.837091 | 6.378970e-57 | 8.197776e-54 |
| MsG0180004064.01 | MsG0480023925.01 | 0.848359 | 6.445972e-60 | 1.179894e-56 |
| MsG0280010888.01 | MsG0480023925.01 | 0.811744 | 6.006883e-51 | 3.748409e-48 |
| MsG0480020928.01 | MsG0480023925.01 | 0.800365 | 1.491583e-48 | 6.932415e-46 |
| MsG0480022968.01 | MsG0480023925.01 | 0.807277 | 5.468870e-50 | 3.033389e-47 |
| MsG0480023925.01 | MsG0580028616.01 | 0.851555 | 8.221556e-61 | 1.670414e-57 |
| MsG0480023925.01 | MsG0780037999.01 | 0.826485 | 2.644257e-54 | 2.482977e-51 |
| MsG0480023925.01 | MsG0780039937.01 | 0.806419 | 8.302274e-50 | 4.503956e-47 |
| MsG0480023925.01 | MsG0780039984.01 | 0.813157 | 2.950337e-51 | 1.911857e-48 |
| MsG0480023925.01 | MsG0780040877.01 | 0.807340 | 5.303352e-50 | 2.946361e-47 |
| MsG0480023925.01 | MsG0780041076.01 | 0.801132 | 1.040212e-48 | 4.929659e-46 |
| MsG0480023925.01 | MsG0880043472.01 | 0.811715 | 6.094689e-51 | 3.800229e-48 |
| MsG0480023925.01 | MsG0880044342.01 | 0.804749 | 1.860469e-49 | 9.670516e-47 |
| MsG0480023925.01 | MsG0880045411.01 | -0.830567 | 2.731630e-55 | 2.888712e-52 |
| MsG0480023925.01 | MsG0880046080.01 | 0.824720 | 6.926659e-54 | 6.186198e-51 |
| MsG0480023925.01 | MsG0880046486.01 | 0.806930 | 6.474690e-50 | 3.559021e-47 |
| MsG0380016183.01 | MsG0480023925.01 | 0.818875 | 1.560025e-52 | 1.181940e-49 |
| MsG0380017637.01 | MsG0480023925.01 | 0.800375 | 1.484553e-48 | 6.901586e-46 |
| MsG0280007084.01 | MsG0480023925.01 | 0.834889 | 2.308422e-56 | 2.776610e-53 |
| MsG0280008263.01 | MsG0480023925.01 | 0.806415 | 8.317518e-50 | 4.511761e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023925.01.T01 | MTR_8g104980 | 100.000 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 4.17e-93 | 265 |
| MsG0480023925.01.T01 | MTR_6g071330 | 40.157 | 127 | 76 | 0 | 1 | 127 | 1 | 127 | 2.10e-29 | 103 |
| MsG0480023925.01.T01 | MTR_5g048060 | 40.157 | 127 | 76 | 0 | 1 | 127 | 1 | 127 | 3.01e-29 | 103 |
| MsG0480023925.01.T01 | MTR_6g071330 | 44.706 | 85 | 47 | 0 | 1 | 85 | 1 | 85 | 1.02e-19 | 78.2 |
| MsG0480023925.01.T01 | MTR_5g048060 | 38.028 | 71 | 44 | 0 | 57 | 127 | 21 | 91 | 1.99e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023925.01.T01 | AT5G17190 | 88.462 | 130 | 15 | 0 | 1 | 130 | 1 | 130 | 1.12e-85 | 246 |
| MsG0480023925.01.T01 | AT3G03160 | 86.154 | 130 | 18 | 0 | 1 | 130 | 1 | 130 | 4.72e-83 | 239 |
| MsG0480023925.01.T01 | AT1G48440 | 44.615 | 130 | 65 | 1 | 1 | 130 | 1 | 123 | 3.09e-32 | 110 |
| MsG0480023925.01.T01 | AT3G17780 | 41.538 | 130 | 69 | 1 | 1 | 130 | 1 | 123 | 6.68e-26 | 94.7 |
Find 34 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCTGATGGACATTTAT+TGG | 0.134696 | 4:+91309943 | MsG0480023925.01.T01:CDS |
| AAACGGTTTGAGAAGATTCT+TGG | 0.339054 | 4:-91309895 | None:intergenic |
| CGGTCCTCTTGAGGCGTTCA+AGG | 0.371336 | 4:-91310148 | None:intergenic |
| AAGAAATTTGGCAATTCATT+CGG | 0.387950 | 4:-91310168 | None:intergenic |
| CTGCTGCGCTTCTCTTCTAC+TGG | 0.392268 | 4:+91310072 | MsG0480023925.01.T01:CDS |
| CGCAGCAGCAGAAGCGATCA+TGG | 0.402746 | 4:+91309815 | MsG0480023925.01.T01:CDS |
| TTGCGAAGAGCGTCGAGGCC+GGG | 0.422996 | 4:-91309855 | None:intergenic |
| ATCGATTCTAACAACAAGAT+TGG | 0.441686 | 4:-91310111 | None:intergenic |
| ATCACCTTCACAGCTTGGCC+TGG | 0.445526 | 4:-91309976 | None:intergenic |
| TGCGAAGAGCGTCGAGGCCG+GGG | 0.453391 | 4:-91309854 | None:intergenic |
| CGGCCTCGACGCTCTTCGCA+AGG | 0.457866 | 4:+91309857 | MsG0480023925.01.T01:CDS |
| CTGAATCAATGGCGTTGGAA+TGG | 0.464316 | 4:+91309775 | None:intergenic |
| TCTTGAGGCGTTCAAGGCGC+TGG | 0.467572 | 4:-91310142 | None:intergenic |
| CAAGAATCACCTTCACAGCT+TGG | 0.469024 | 4:-91309981 | None:intergenic |
| CAATTCATTCGGTCCTCTTG+AGG | 0.472139 | 4:-91310157 | None:intergenic |
| CTTGCGAAGAGCGTCGAGGC+CGG | 0.486698 | 4:-91309856 | None:intergenic |
| TGAATCAATGGCGTTGGAAT+GGG | 0.503262 | 4:+91309776 | None:intergenic |
| AACGGAACCACCGAGAGAAA+CGG | 0.508904 | 4:-91309912 | None:intergenic |
| ATCAGAAGAAAGAGGCAAAA+CGG | 0.519304 | 4:-91309930 | None:intergenic |
| TCTTCTCAAACCGTTTCTCT+CGG | 0.531649 | 4:+91309902 | MsG0480023925.01.T01:CDS |
| AGCGCCTTGAACGCCTCAAG+AGG | 0.535773 | 4:+91310144 | MsG0480023925.01.T01:CDS |
| CATGATCGCTTCTGCTGCTG+CGG | 0.545061 | 4:-91309814 | None:intergenic |
| AAATGTCCATCAGAAGAAAG+AGG | 0.548010 | 4:-91309938 | None:intergenic |
| TCTCAAACCGTTTCTCTCGG+TGG | 0.553740 | 4:+91309905 | MsG0480023925.01.T01:CDS |
| AGGTGATTCTTGCACTCCAT+CGG | 0.561627 | 4:+91309992 | MsG0480023925.01.T01:CDS |
| GGCCTCGACGCTCTTCGCAA+GGG | 0.573478 | 4:+91309858 | MsG0480023925.01.T01:CDS |
| GAAACCAGGCCAAGCTGTGA+AGG | 0.575504 | 4:+91309972 | MsG0480023925.01.T01:CDS |
| GAGATCTGAATCAATGGCGT+TGG | 0.578135 | 4:+91309770 | None:intergenic |
| TGATGACGAAGATGTTCCGA+TGG | 0.580742 | 4:-91310008 | None:intergenic |
| GCGTTGGAATGGGTTGTGCT+AGG | 0.592663 | 4:+91309786 | MsG0480023925.01.T01:CDS |
| TTTATTGGAAATACGAAACC+AGG | 0.610945 | 4:+91309958 | MsG0480023925.01.T01:CDS |
| TCTAACAACAAGATTGGTAA+CGG | 0.614653 | 4:-91310105 | None:intergenic |
| GTGCTTCTTCTCACAATCCC+CGG | 0.623193 | 4:+91309837 | MsG0480023925.01.T01:CDS |
| GTCCCTTGCGAAGAGCGTCG+AGG | 0.706877 | 4:-91309860 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTAACAACAAGATTGGTAA+CGG | - | Chr4:91310108-91310127 | None:intergenic | 30.0% | |
| TTTATTGGAAATACGAAACC+AGG | + | Chr4:91309958-91309977 | MsG0480023925.01.T01:CDS | 30.0% | |
| ! | ATCGATTCTAACAACAAGAT+TGG | - | Chr4:91310114-91310133 | None:intergenic | 30.0% |
| ! | TTCTTCTGATGGACATTTAT+TGG | + | Chr4:91309943-91309962 | MsG0480023925.01.T01:CDS | 30.0% |
| AAACGGTTTGAGAAGATTCT+TGG | - | Chr4:91309898-91309917 | None:intergenic | 35.0% | |
| AAATGTCCATCAGAAGAAAG+AGG | - | Chr4:91309941-91309960 | None:intergenic | 35.0% | |
| ATCAGAAGAAAGAGGCAAAA+CGG | - | Chr4:91309933-91309952 | None:intergenic | 35.0% | |
| TCTTCTCAAACCGTTTCTCT+CGG | + | Chr4:91309902-91309921 | MsG0480023925.01.T01:CDS | 40.0% | |
| ! | GTTTTGCCTCTTTCTTCTGA+TGG | + | Chr4:91309932-91309951 | MsG0480023925.01.T01:CDS | 40.0% |
| AGGTGATTCTTGCACTCCAT+CGG | + | Chr4:91309992-91310011 | MsG0480023925.01.T01:CDS | 45.0% | |
| CAAGAATCACCTTCACAGCT+TGG | - | Chr4:91309984-91310003 | None:intergenic | 45.0% | |
| ! | TGATGACGAAGATGTTCCGA+TGG | - | Chr4:91310011-91310030 | None:intergenic | 45.0% |
| AACGGAACCACCGAGAGAAA+CGG | - | Chr4:91309915-91309934 | None:intergenic | 50.0% | |
| GTGCTTCTTCTCACAATCCC+CGG | + | Chr4:91309837-91309856 | MsG0480023925.01.T01:CDS | 50.0% | |
| TCTCAAACCGTTTCTCTCGG+TGG | + | Chr4:91309905-91309924 | MsG0480023925.01.T01:CDS | 50.0% | |
| ATCACCTTCACAGCTTGGCC+TGG | - | Chr4:91309979-91309998 | None:intergenic | 55.0% | |
| CATGATCGCTTCTGCTGCTG+CGG | - | Chr4:91309817-91309836 | None:intergenic | 55.0% | |
| CTGCTGCGCTTCTCTTCTAC+TGG | + | Chr4:91310072-91310091 | MsG0480023925.01.T01:CDS | 55.0% | |
| GAAACCAGGCCAAGCTGTGA+AGG | + | Chr4:91309972-91309991 | MsG0480023925.01.T01:CDS | 55.0% | |
| !! | GCGTTGGAATGGGTTGTGCT+AGG | + | Chr4:91309786-91309805 | MsG0480023925.01.T01:CDS | 55.0% |
| AGCGCCTTGAACGCCTCAAG+AGG | + | Chr4:91310144-91310163 | MsG0480023925.01.T01:CDS | 60.0% | |
| CGCAGCAGCAGAAGCGATCA+TGG | + | Chr4:91309815-91309834 | MsG0480023925.01.T01:CDS | 60.0% | |
| ! | CGGTCCTCTTGAGGCGTTCA+AGG | - | Chr4:91310151-91310170 | None:intergenic | 60.0% |
| ! | TCTTGAGGCGTTCAAGGCGC+TGG | - | Chr4:91310145-91310164 | None:intergenic | 60.0% |
| GGCCTCGACGCTCTTCGCAA+GGG | + | Chr4:91309858-91309877 | MsG0480023925.01.T01:CDS | 65.0% | |
| !! | CTTGCGAAGAGCGTCGAGGC+CGG | - | Chr4:91309859-91309878 | None:intergenic | 65.0% |
| !! | GTCCCTTGCGAAGAGCGTCG+AGG | - | Chr4:91309863-91309882 | None:intergenic | 65.0% |
| !! | TTGCGAAGAGCGTCGAGGCC+GGG | - | Chr4:91309858-91309877 | None:intergenic | 65.0% |
| CGGCCTCGACGCTCTTCGCA+AGG | + | Chr4:91309857-91309876 | MsG0480023925.01.T01:CDS | 70.0% | |
| !! | TGCGAAGAGCGTCGAGGCCG+GGG | - | Chr4:91309857-91309876 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 91309783 | 91310175 | 91309783 | ID=MsG0480023925.01;Name=MsG0480023925.01 |
| Chr4 | mRNA | 91309783 | 91310175 | 91309783 | ID=MsG0480023925.01.T01;Parent=MsG0480023925.01;Name=MsG0480023925.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|130 |
| Chr4 | exon | 91309783 | 91310175 | 91309783 | ID=MsG0480023925.01.T01:exon:13257;Parent=MsG0480023925.01.T01 |
| Chr4 | CDS | 91309783 | 91310175 | 91309783 | ID=MsG0480023925.01.T01:cds;Parent=MsG0480023925.01.T01 |
| Gene Sequence |
| Protein sequence |
