AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028755.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028755.01.T01 MTR_5g076080 98.551 207 3 0 46 252 48 254 7.05e-151 421
MsG0580028755.01.T01 MTR_5g076080 98.537 205 3 0 48 252 1 205 1.40e-149 416
MsG0580028755.01.T01 MTR_5g076010 90.756 119 11 0 48 166 1 119 9.86e-69 221
MsG0580028755.01.T01 MTR_3g111940 56.962 158 66 2 95 252 49 204 3.10e-58 184
MsG0580028755.01.T01 MTR_6g037360 85.417 96 14 0 48 143 1 96 6.75e-54 170
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028755.01.T01 AT5G10860 81.553 206 37 1 48 252 1 206 5.44e-123 348
MsG0580028755.01.T01 AT1G47271 47.340 188 97 2 65 252 7 192 2.30e-55 176

Find 94 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCCCGACCTTTGTGGATT+TGG 0.190734 5:+85888941 None:intergenic
GCCTTGTCTATAGTACATTT+TGG 0.204877 5:+85891035 None:intergenic
TCGCAATCCTCCTTGCATTT+TGG 0.238709 5:+85889736 None:intergenic
TTCGAAGAATTTCAATGTAT+TGG 0.255811 5:-85887684 MsG0580028755.01.T01:three_prime_UTR
TCGAAGAATTTCAATGTATT+GGG 0.267356 5:-85887683 MsG0580028755.01.T01:three_prime_UTR
GCTCGCAGAACTTTGGTATC+AGG 0.270882 5:+85888584 None:intergenic
AGTGAGAGAGAGTGGCTTTC+GGG 0.278653 5:+85890924 None:intergenic
AATAATAATAAATAATAGTT+TGG 0.302327 5:-85891071 None:intergenic
TGAGAGTTGTAAATATGTTT+TGG 0.334070 5:-85887968 MsG0580028755.01.T01:three_prime_UTR
GCGCTGATGGTTCCTGGCTT+TGG 0.334908 5:-85889535 MsG0580028755.01.T01:CDS
GAGTGAGAGAGAGTGGCTTT+CGG 0.336786 5:+85890923 None:intergenic
GTAAAGGCGCTGATGGTTCC+TGG 0.372640 5:-85889541 MsG0580028755.01.T01:CDS
TAACTTCTACTGTTTGCAAT+TGG 0.378955 5:-85887799 MsG0580028755.01.T01:three_prime_UTR
AAAAGGAAACTGGCTGCAAC+AGG 0.390868 5:+85889648 None:intergenic
GCAATTGATTTCTCCTCCCC+TGG 0.398515 5:+85889343 None:intergenic
CATAATATCCCCGACCTTTG+TGG 0.405140 5:+85888935 None:intergenic
CGCTTGAATGCTTACATACA+AGG 0.428996 5:-85888072 MsG0580028755.01.T01:CDS
TCAAACCGCGAAAAGGAAAC+TGG 0.429891 5:+85889638 None:intergenic
TCAGACATCTTGCAAGGAAA+AGG 0.429987 5:-85889563 MsG0580028755.01.T01:CDS
TGTATGCATCTTGTTTCTGT+TGG 0.440766 5:-85887848 MsG0580028755.01.T01:three_prime_UTR
CTGCATTGCTCGCAGAACTT+TGG 0.445326 5:+85888577 None:intergenic
GGGGAGGAGAAATCAATTGC+TGG 0.452072 5:-85889340 MsG0580028755.01.T01:CDS
CCACTCTCTCTCACTCTCTC+TGG 0.456070 5:-85890916 MsG0580028755.01.T01:intron
ATTTCCTTGTCATTTATGAC+AGG 0.461486 5:+85888162 None:intergenic
ACAAAACAATGTTGGTGCTT+TGG 0.467003 5:-85889377 MsG0580028755.01.T01:CDS
CATCCAAATCCACAAAGGTC+GGG 0.470096 5:-85888944 MsG0580028755.01.T01:CDS
ATCTTACAAAATTGTGAAGT+GGG 0.474214 5:+85891013 None:intergenic
TGTTTATGATGCTGTCAAAT+CGG 0.475200 5:-85889495 MsG0580028755.01.T01:intron
GCTTTGGTGGTCGTGAAACC+AGG 0.480001 5:-85889361 MsG0580028755.01.T01:CDS
GAGCTGAAATCCGAAATTAC+AGG 0.487042 5:+85887917 None:intergenic
TGCTACACCTGCTCGTATTG+AGG 0.488020 5:-85889612 MsG0580028755.01.T01:CDS
CGAGCAATGCAGCTGATGAC+TGG 0.490351 5:-85888566 MsG0580028755.01.T01:intron
CTCGCAGAACTTTGGTATCA+GGG 0.492406 5:+85888585 None:intergenic
AGTTAGCTTGAGTGCCCTCA+TGG 0.496067 5:+85888009 None:intergenic
AGAGTGGCTTTCGGGTAGAA+TGG 0.499627 5:+85890932 None:intergenic
ATCTGTGGTGCACCAAAGCC+AGG 0.513664 5:+85889523 None:intergenic
ATGCTCGGCATGGTGTCCAT+TGG 0.515005 5:-85888141 MsG0580028755.01.T01:CDS
ACAGTAGAAGTTAAAGATGA+AGG 0.516626 5:+85887809 None:intergenic
GCTGGAATTATAACAGAAAG+AGG 0.519325 5:-85889322 MsG0580028755.01.T01:intron
AGCAGATTCAAACCGCGAAA+AGG 0.521926 5:+85889631 None:intergenic
ATTATTAACAAGCTACTCCA+TGG 0.525573 5:+85887877 None:intergenic
AACAGTGGTTCATACCTCTT+CGG 0.527383 5:+85888911 None:intergenic
TGAGGTGGTGAGTGAGTGAG+AGG 0.529852 5:+85890961 None:intergenic
AAGGAAACTGGCTGCAACAG+GGG 0.534668 5:+85889650 None:intergenic
CCTGAGGAAGATCATCGTGC+AGG 0.535472 5:-85888973 MsG0580028755.01.T01:CDS
CCTGCACGATGATCTTCCTC+AGG 0.537692 5:+85888973 None:intergenic
AATTTCGGATTTCAGCTCCA+TGG 0.539998 5:-85887912 MsG0580028755.01.T01:three_prime_UTR
TCATCCAAATCCACAAAGGT+CGG 0.550703 5:-85888945 MsG0580028755.01.T01:CDS
AATCTTACAAAATTGTGAAG+TGG 0.552382 5:+85891012 None:intergenic
ATCCAAATCCACAAAGGTCG+GGG 0.553551 5:-85888943 MsG0580028755.01.T01:CDS
CCAGAGAGAGTGAGAGAGAG+TGG 0.556488 5:+85890916 None:intergenic
AAACAATGTTGGTGCTTTGG+TGG 0.560729 5:-85889374 MsG0580028755.01.T01:CDS
ATCTTGCAAGGAAAAGGTAA+AGG 0.561654 5:-85889557 MsG0580028755.01.T01:CDS
CGGGTAGAATGGAATGGTTG+AGG 0.562430 5:+85890943 None:intergenic
GGAAAAGGTAAAGGCGCTGA+TGG 0.566636 5:-85889548 MsG0580028755.01.T01:CDS
TTGCAAGATGTCTGAAATTG+TGG 0.570234 5:+85889571 None:intergenic
GGCTTTCGGGTAGAATGGAA+TGG 0.570921 5:+85890937 None:intergenic
CATGGATTAATGTTAATCCA+TGG 0.571571 5:+85887895 None:intergenic
CCATGCTCCTCAATACGAGC+AGG 0.573052 5:+85889605 None:intergenic
AGATGTTGTTCGTGCTGTCG+TGG 0.574794 5:-85888118 MsG0580028755.01.T01:CDS
GATGTTGTTCGTGCTGTCGT+GGG 0.578243 5:-85888117 MsG0580028755.01.T01:CDS
ACAATTTCAGACATCTTGCA+AGG 0.588745 5:-85889569 MsG0580028755.01.T01:CDS
TTTCCTTGTCATTTATGACA+GGG 0.591475 5:+85888163 None:intergenic
ATAAATGACAAGGAAATGCT+CGG 0.596746 5:-85888156 MsG0580028755.01.T01:CDS
CTTTGGTGGTCGTGAAACCA+GGG 0.598133 5:-85889360 MsG0580028755.01.T01:CDS
AGCACGAACAACATCTCCAA+TGG 0.603380 5:+85888125 None:intergenic
CATGGATTAACATTAATCCA+TGG 0.603682 5:-85887894 MsG0580028755.01.T01:three_prime_UTR
GTTAGCTTGAGTGCCCTCAT+GGG 0.605065 5:+85888010 None:intergenic
CTGTAAGAACTATTTGCAGA+AGG 0.605436 5:-85887653 MsG0580028755.01.T01:three_prime_UTR
CATCCCTGTCATAAATGACA+AGG 0.612336 5:-85888166 MsG0580028755.01.T01:CDS
CTGAGGAAGATCATCGTGCA+GGG 0.615519 5:-85888972 MsG0580028755.01.T01:CDS
CCTGCTCGTATTGAGGAGCA+TGG 0.625826 5:-85889605 MsG0580028755.01.T01:CDS
ATCATAAACAGTGTCATCTG+TGG 0.626836 5:+85889508 None:intergenic
TGACAAGGAAATGCTCGGCA+TGG 0.629220 5:-85888151 MsG0580028755.01.T01:CDS
CAGATGACACAAAACAATGT+TGG 0.632418 5:-85889385 MsG0580028755.01.T01:CDS
TATTGGACAGCCAAAATGCA+AGG 0.632617 5:-85889746 MsG0580028755.01.T01:intron
TCCAAAATGTACTATAGACA+AGG 0.634672 5:-85891036 MsG0580028755.01.T01:exon
GCTTGAATGCTTACATACAA+GGG 0.636806 5:-85888071 MsG0580028755.01.T01:CDS
TGATGATGAAAATCCCATGA+GGG 0.644662 5:-85888023 MsG0580028755.01.T01:three_prime_UTR
CTTGAATGCTTACATACAAG+GGG 0.645653 5:-85888070 MsG0580028755.01.T01:CDS
TCGCAGAACTTTGGTATCAG+GGG 0.648663 5:+85888586 None:intergenic
CTTGTATGTAAGCATTCAAG+CGG 0.650554 5:+85888073 None:intergenic
AAAGGAAACTGGCTGCAACA+GGG 0.650797 5:+85889649 None:intergenic
GAGGTGGTGAGTGAGTGAGA+GGG 0.656104 5:+85890962 None:intergenic
CGGGGATATTATGACCGAAG+AGG 0.658203 5:-85888925 MsG0580028755.01.T01:intron
GTAGAATGGAATGGTTGAGG+TGG 0.683439 5:+85890946 None:intergenic
TCTACTATCTAGATTACCTG+AGG 0.687591 5:-85888989 MsG0580028755.01.T01:intron
GCAACAGGGGATTCACCATG+CGG 0.688778 5:+85889663 None:intergenic
AAGATCATCCAAATCCACAA+AGG 0.697260 5:-85888949 MsG0580028755.01.T01:CDS
GGTGGTCGTGAAACCAGGGG+AGG 0.700076 5:-85889356 MsG0580028755.01.T01:CDS
TTTGGTGGTCGTGAAACCAG+GGG 0.702988 5:-85889359 MsG0580028755.01.T01:CDS
TGGACAGCCAAAATGCAAGG+AGG 0.724659 5:-85889743 MsG0580028755.01.T01:intron
ATGATGATGAAAATCCCATG+AGG 0.725297 5:-85888024 MsG0580028755.01.T01:three_prime_UTR
TTGAATGCTTACATACAAGG+GGG 0.750705 5:-85888069 MsG0580028755.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTTATTTTTATTTT+TGG - Chr5:85888287-85888306 MsG0580028755.01.T01:intron 0.0%
!! AAATATGTATATTAGATAAC+AGG - Chr5:85888626-85888645 MsG0580028755.01.T01:intron 15.0%
!! AAGAACAATATGATTTATAT+TGG - Chr5:85889483-85889502 MsG0580028755.01.T01:intron 15.0%
!! ATAATAATGATCAAGTATAT+GGG - Chr5:85887934-85887953 MsG0580028755.01.T01:three_prime_UTR 15.0%
!! TATAAATTTCATATTCTAGA+GGG - Chr5:85888782-85888801 MsG0580028755.01.T01:intron 15.0%
!! TTATAAATTTCATATTCTAG+AGG - Chr5:85888781-85888800 MsG0580028755.01.T01:intron 15.0%
!!! AGATAAAATTTTGATTGTTT+TGG - Chr5:85891054-85891073 MsG0580028755.01.T01:five_prime_UTR 15.0%
!! AAAAGCAAGAAAAATAGTAA+TGG + Chr5:85887801-85887820 None:intergenic 20.0%
!! AAATCGATAATTTGAAAATG+TGG + Chr5:85889992-85890011 None:intergenic 20.0%
!! ACGTTAATGACAAATATAAT+TGG + Chr5:85889634-85889653 None:intergenic 20.0%
!! AGATCTATACTTTATCAAAA+AGG + Chr5:85887863-85887882 None:intergenic 20.0%
!! ATGAAATTTATAAGTGATTC+TGG + Chr5:85888774-85888793 None:intergenic 20.0%
!! ATGGATTTGATTGAATTTAT+TGG - Chr5:85888038-85888057 MsG0580028755.01.T01:three_prime_UTR 20.0%
!! GATAATAATGATCAAGTATA+TGG - Chr5:85887933-85887952 MsG0580028755.01.T01:three_prime_UTR 20.0%
!! GATCTATACTTTATCAAAAA+GGG + Chr5:85887862-85887881 None:intergenic 20.0%
!! TTGATTTACTTCATTTAGAA+AGG + Chr5:85888366-85888385 None:intergenic 20.0%
!!! AAATGAAGGTTTTTTTTTCA+TGG - Chr5:85888019-85888038 MsG0580028755.01.T01:three_prime_UTR 20.0%
!!! AGAAAAATGCTTTGAATAAA+AGG - Chr5:85888173-85888192 MsG0580028755.01.T01:CDS 20.0%
!!! ATTATGTTTTTGCAATGATT+GGG - Chr5:85888217-85888236 MsG0580028755.01.T01:intron 20.0%
!!! CAATTCTCTAAAAATTAGTA+TGG + Chr5:85889830-85889849 None:intergenic 20.0%
!!! GAAAAATGCTTTGAATAAAA+GGG - Chr5:85888174-85888193 MsG0580028755.01.T01:CDS 20.0%
!!! TATTATGTTTTTGCAATGAT+TGG - Chr5:85888216-85888235 MsG0580028755.01.T01:intron 20.0%
!!! TGTTTTTTTTCCTGTAATTT+CGG - Chr5:85890731-85890750 MsG0580028755.01.T01:intron 20.0%
!!! TTGTTGTATAATGGATAATT+TGG - Chr5:85889398-85889417 MsG0580028755.01.T01:intron 20.0%
! AAAAATTTGAGTGCTAAATC+AGG - Chr5:85887908-85887927 MsG0580028755.01.T01:three_prime_UTR 25.0%
! AATAAGCAGATAACAACTTA+TGG - Chr5:85890334-85890353 MsG0580028755.01.T01:intron 25.0%
! AATAGTCAAATAAGTAACAG+TGG + Chr5:85889765-85889784 None:intergenic 25.0%
! AATCTTACAAAATTGTGAAG+TGG + Chr5:85887649-85887668 None:intergenic 25.0%
! ACACATAATATTTGCCAATT+TGG + Chr5:85889882-85889901 None:intergenic 25.0%
! ATCTTACAAAATTGTGAAGT+GGG + Chr5:85887648-85887667 None:intergenic 25.0%
! ATGACAAATATAATTGGAAG+TGG + Chr5:85889628-85889647 None:intergenic 25.0%
! GATCATGGAAAAACAAAAAA+TGG + Chr5:85887831-85887850 None:intergenic 25.0%
! GTTGTCTTATATGTATGTAT+TGG - Chr5:85888804-85888823 MsG0580028755.01.T01:intron 25.0%
! TACTACATTGAAATTCTAAG+AGG - Chr5:85888878-85888897 MsG0580028755.01.T01:intron 25.0%
! TCGAAGAATTTCAATGTATT+GGG - Chr5:85890975-85890994 MsG0580028755.01.T01:CDS 25.0%
! TGAGATTCTTTATGATGTTA+TGG - Chr5:85888461-85888480 MsG0580028755.01.T01:intron 25.0%
! TTAAGCTATTAATCCAAACA+AGG + Chr5:85890240-85890259 None:intergenic 25.0%
! TTCGAAGAATTTCAATGTAT+TGG - Chr5:85890974-85890993 MsG0580028755.01.T01:CDS 25.0%
!! ATTTAGCACTCAAATTTTTC+TGG + Chr5:85887907-85887926 None:intergenic 25.0%
!! TCACAATTTTGTAAGATTTG+AGG - Chr5:85887651-85887670 MsG0580028755.01.T01:three_prime_UTR 25.0%
!! TGAGAGTTGTAAATATGTTT+TGG - Chr5:85890690-85890709 MsG0580028755.01.T01:intron 25.0%
!! TGTTTGATATTTCTATCCAA+AGG + Chr5:85890138-85890157 None:intergenic 25.0%
!! TTTAGCACTCAAATTTTTCT+GGG + Chr5:85887906-85887925 None:intergenic 25.0%
!!! AGAGCTTTGAAATTAAATGA+AGG - Chr5:85888005-85888024 MsG0580028755.01.T01:three_prime_UTR 25.0%
!!! ATCTATTTCTGTATTGGATT+TGG - Chr5:85888673-85888692 MsG0580028755.01.T01:intron 25.0%
!!! ATTTGATGGATTTGGTTTTT+TGG - Chr5:85888120-85888139 MsG0580028755.01.T01:CDS 25.0%
!!! CTGGTATTGATATGTTTTTT+CGG - Chr5:85890111-85890130 MsG0580028755.01.T01:intron 25.0%
!!! GGTAAAATCTATTTCTGTAT+TGG - Chr5:85888667-85888686 MsG0580028755.01.T01:intron 25.0%
!!! TATTTTTTCTTCCAGATAAC+AGG - Chr5:85890461-85890480 MsG0580028755.01.T01:intron 25.0%
!!! TCTTTTCTTGTTTATATCCA+CGG - Chr5:85888583-85888602 MsG0580028755.01.T01:CDS 25.0%
!!! TTAAAAATGCTGTTTTGCAA+CGG - Chr5:85888970-85888989 MsG0580028755.01.T01:CDS 25.0%
!!! TTATGTTTTTGCAATGATTG+GGG - Chr5:85888218-85888237 MsG0580028755.01.T01:intron 25.0%
!!! TTTGATGGATTTGGTTTTTT+GGG - Chr5:85888121-85888140 MsG0580028755.01.T01:CDS 25.0%
!!! TTTTGATTTCTTTTTGCACT+TGG - Chr5:85888426-85888445 MsG0580028755.01.T01:intron 25.0%
AACAATATTGTTGGCATGAA+GGG - Chr5:85888701-85888720 MsG0580028755.01.T01:intron 30.0%
ACAACTCGACATCTTAAATA+AGG - Chr5:85890192-85890211 MsG0580028755.01.T01:intron 30.0%
ACAGTAGAAGTTAAAGATGA+AGG + Chr5:85890852-85890871 None:intergenic 30.0%
ACCAATGATAAACCAATAGA+TGG + Chr5:85888510-85888529 None:intergenic 30.0%
AGGAAAAAAAACAGACAAGA+TGG + Chr5:85890724-85890743 None:intergenic 30.0%
ATAAATGACAAGGAAATGCT+CGG - Chr5:85890502-85890521 MsG0580028755.01.T01:intron 30.0%
ATAATATTTGCCAATTTGGC+AGG + Chr5:85889878-85889897 None:intergenic 30.0%
ATTAGTATGGCACAAAATGT+AGG + Chr5:85889817-85889836 None:intergenic 30.0%
ATTATTAACAAGCTACTCCA+TGG + Chr5:85890784-85890803 None:intergenic 30.0%
CATTTCTCATTTCCATCTAT+TGG - Chr5:85888495-85888514 MsG0580028755.01.T01:intron 30.0%
GAAATAACTGCATGAACATT+TGG + Chr5:85889364-85889383 None:intergenic 30.0%
GAATAAGAATCTGTTTGGAT+TGG + Chr5:85890426-85890445 None:intergenic 30.0%
GACTAGAATAAGAATCTGTT+TGG + Chr5:85890431-85890450 None:intergenic 30.0%
GATATTTGCTTACATCAAAC+TGG - Chr5:85889433-85889452 MsG0580028755.01.T01:intron 30.0%
GCACATATTCATGTAAGAAA+AGG + Chr5:85888849-85888868 None:intergenic 30.0%
GTATATTAGATAACAGGTTG+TGG - Chr5:85888632-85888651 MsG0580028755.01.T01:intron 30.0%
TAACTTCTACTGTTTGCAAT+TGG - Chr5:85890859-85890878 MsG0580028755.01.T01:intron 30.0%
TAATATTTGCCAATTTGGCA+GGG + Chr5:85889877-85889896 None:intergenic 30.0%
TCCAAAATGTACTATAGACA+AGG - Chr5:85887622-85887641 MsG0580028755.01.T01:three_prime_UTR 30.0%
TGTTTATGATGCTGTCAAAT+CGG - Chr5:85889163-85889182 MsG0580028755.01.T01:intron 30.0%
TTCAGCTCAAATTAACAACA+TGG + Chr5:85888148-85888167 None:intergenic 30.0%
TTGATATTGCCTATTAAGCA+GGG - Chr5:85889934-85889953 MsG0580028755.01.T01:intron 30.0%
TTGGTGAAGAACAATATTGT+TGG - Chr5:85888692-85888711 MsG0580028755.01.T01:intron 30.0%
! ATTTCCTTGTCATTTATGAC+AGG + Chr5:85890499-85890518 None:intergenic 30.0%
! CATGGATTAACATTAATCCA+TGG - Chr5:85890764-85890783 MsG0580028755.01.T01:intron 30.0%
! CATGGATTAATGTTAATCCA+TGG + Chr5:85890766-85890785 None:intergenic 30.0%
! GACTGTTCTTTGTTGTATAA+TGG - Chr5:85889389-85889408 MsG0580028755.01.T01:intron 30.0%
! GCATGCTTTTGTCAATATAT+TGG - Chr5:85889572-85889591 MsG0580028755.01.T01:CDS 30.0%
! TTTCCTTGTCATTTATGACA+GGG + Chr5:85890498-85890517 None:intergenic 30.0%
! TTTTGTCAATATATTGGACG+CGG - Chr5:85889578-85889597 MsG0580028755.01.T01:CDS 30.0%
!! AAGAGGCACTATTTGATTAT+TGG - Chr5:85888895-85888914 MsG0580028755.01.T01:intron 30.0%
!! AAGGACTAATTTGATGGATT+TGG - Chr5:85888112-85888131 MsG0580028755.01.T01:CDS 30.0%
!! GTTCATGCAGTTATTTCTTT+GGG - Chr5:85889367-85889386 MsG0580028755.01.T01:CDS 30.0%
!! TATAAGTGATTCTGGAATCA+CGG + Chr5:85888766-85888785 None:intergenic 30.0%
!! TATGTTTTTGCAATGATTGG+GGG - Chr5:85888219-85888238 MsG0580028755.01.T01:intron 30.0%
!! TCCATCTATTGGTTTATCAT+TGG - Chr5:85888506-85888525 MsG0580028755.01.T01:intron 30.0%
!! TGTTCATGCAGTTATTTCTT+TGG - Chr5:85889366-85889385 MsG0580028755.01.T01:CDS 30.0%
!! TTGCGATCATTTTTGTCAAA+CGG - Chr5:85888939-85888958 MsG0580028755.01.T01:CDS 30.0%
!!! TAAAAATGCTGTTTTGCAAC+GGG - Chr5:85888971-85888990 MsG0580028755.01.T01:CDS 30.0%
AAGATCATCCAAATCCACAA+AGG - Chr5:85889709-85889728 MsG0580028755.01.T01:CDS 35.0%
AAGCTATCATGAAGAGCTTA+TGG - Chr5:85890310-85890329 MsG0580028755.01.T01:intron 35.0%
AATATTTGCCAATTTGGCAG+GGG + Chr5:85889876-85889895 None:intergenic 35.0%
ACAATTTCAGACATCTTGCA+AGG - Chr5:85889089-85889108 MsG0580028755.01.T01:intron 35.0%
ATACTGATGTATGTGACATG+TGG - Chr5:85888071-85888090 MsG0580028755.01.T01:CDS 35.0%
ATCATAAACAGTGTCATCTG+TGG + Chr5:85889153-85889172 None:intergenic 35.0%
ATCTTGCAAGGAAAAGGTAA+AGG - Chr5:85889101-85889120 MsG0580028755.01.T01:intron 35.0%
CAGATGACACAAAACAATGT+TGG - Chr5:85889273-85889292 MsG0580028755.01.T01:intron 35.0%
CATTATGACACGTCAGTTAA+TGG + Chr5:85889537-85889556 None:intergenic 35.0%
CTGTAAGAACTATTTGCAGA+AGG - Chr5:85891005-85891024 MsG0580028755.01.T01:CDS 35.0%
CTTGAATGCTTACATACAAG+GGG - Chr5:85890588-85890607 MsG0580028755.01.T01:intron 35.0%
CTTGTATGTAAGCATTCAAG+CGG + Chr5:85890588-85890607 None:intergenic 35.0%
GAACAATATTGTTGGCATGA+AGG - Chr5:85888700-85888719 MsG0580028755.01.T01:intron 35.0%
GCTGGAATTATAACAGAAAG+AGG - Chr5:85889336-85889355 MsG0580028755.01.T01:CDS 35.0%
GCTTGAATGCTTACATACAA+GGG - Chr5:85890587-85890606 MsG0580028755.01.T01:intron 35.0%
GTTGATATTGCCTATTAAGC+AGG - Chr5:85889933-85889952 MsG0580028755.01.T01:intron 35.0%
GTTGTAGCATGTTTCACTAT+AGG - Chr5:85890214-85890233 MsG0580028755.01.T01:intron 35.0%
TACTGATGTATGTGACATGT+GGG - Chr5:85888072-85888091 MsG0580028755.01.T01:CDS 35.0%
TCTACTATCTAGATTACCTG+AGG - Chr5:85889669-85889688 MsG0580028755.01.T01:CDS 35.0%
TTAACTGACGTGTCATAATG+TGG - Chr5:85889537-85889556 MsG0580028755.01.T01:CDS 35.0%
TTGAATGCTTACATACAAGG+GGG - Chr5:85890589-85890608 MsG0580028755.01.T01:intron 35.0%
TTTGTCAATATATTGGACGC+GGG - Chr5:85889579-85889598 MsG0580028755.01.T01:CDS 35.0%
! ACAAAACAATGTTGGTGCTT+TGG - Chr5:85889281-85889300 MsG0580028755.01.T01:intron 35.0%
! ATGATGATGAAAATCCCATG+AGG - Chr5:85890634-85890653 MsG0580028755.01.T01:intron 35.0%
! GCCTTGTCTATAGTACATTT+TGG + Chr5:85887626-85887645 None:intergenic 35.0%
! TGATGATGAAAATCCCATGA+GGG - Chr5:85890635-85890654 MsG0580028755.01.T01:intron 35.0%
! TTGCAAGATGTCTGAAATTG+TGG + Chr5:85889090-85889109 None:intergenic 35.0%
!! TGCGATCATTTTTGTCAAAC+GGG - Chr5:85888940-85888959 MsG0580028755.01.T01:CDS 35.0%
!! TGTATGCATCTTGTTTCTGT+TGG - Chr5:85890810-85890829 MsG0580028755.01.T01:intron 35.0%
!!! GATATGTTTTTTCGGTCCTT+TGG - Chr5:85890119-85890138 MsG0580028755.01.T01:intron 35.0%
AACAGTGGTTCATACCTCTT+CGG + Chr5:85889750-85889769 None:intergenic 40.0%
AATTTCGGATTTCAGCTCCA+TGG - Chr5:85890746-85890765 MsG0580028755.01.T01:intron 40.0%
ACCAGTAATTAGGGCAACAT+AGG - Chr5:85889958-85889977 MsG0580028755.01.T01:intron 40.0%
ACTGATGTATGTGACATGTG+GGG - Chr5:85888073-85888092 MsG0580028755.01.T01:CDS 40.0%
AGGTTGTGGTGAAGAACATT+TGG - Chr5:85888646-85888665 MsG0580028755.01.T01:intron 40.0%
ATCCACGGTTAAACAGAACT+TGG - Chr5:85888598-85888617 MsG0580028755.01.T01:CDS 40.0%
ATGTGTCTGATCCTGTTATC+TGG + Chr5:85890475-85890494 None:intergenic 40.0%
CATCCCTGTCATAAATGACA+AGG - Chr5:85890492-85890511 MsG0580028755.01.T01:intron 40.0%
CGCTTGAATGCTTACATACA+AGG - Chr5:85890586-85890605 MsG0580028755.01.T01:intron 40.0%
GAGCTGAAATCCGAAATTAC+AGG + Chr5:85890744-85890763 None:intergenic 40.0%
GCACACGACACATTCAAATT+TGG + Chr5:85889459-85889478 None:intergenic 40.0%
GTGGTGAAGGACTAATTTGA+TGG - Chr5:85888106-85888125 MsG0580028755.01.T01:CDS 40.0%
TACCAAGTTCTGTTTAACCG+TGG + Chr5:85888603-85888622 None:intergenic 40.0%
TATTGGACAGCCAAAATGCA+AGG - Chr5:85888912-85888931 MsG0580028755.01.T01:intron 40.0%
TCAGACATCTTGCAAGGAAA+AGG - Chr5:85889095-85889114 MsG0580028755.01.T01:intron 40.0%
TCATCCAAATCCACAAAGGT+CGG - Chr5:85889713-85889732 MsG0580028755.01.T01:CDS 40.0%
TCCTATGTTGCCCTAATTAC+TGG + Chr5:85889962-85889981 None:intergenic 40.0%
TTACTGGTACCCTGCTTAAT+AGG + Chr5:85889946-85889965 None:intergenic 40.0%
! AAAAGGGTGTGCATAGATCA+TGG + Chr5:85887846-85887865 None:intergenic 40.0%
! AAACAATGTTGGTGCTTTGG+TGG - Chr5:85889284-85889303 MsG0580028755.01.T01:intron 40.0%
! AAGCAGGGTACCAGTAATTA+GGG - Chr5:85889949-85889968 MsG0580028755.01.T01:intron 40.0%
! AGAAAAGGTGACGAGTTTTG+AGG + Chr5:85888834-85888853 None:intergenic 40.0%
! GGTAGTTCTGTATGATCTTG+TGG - Chr5:85888527-85888546 MsG0580028755.01.T01:intron 40.0%
! TAAGCAGGGTACCAGTAATT+AGG - Chr5:85889948-85889967 MsG0580028755.01.T01:intron 40.0%
! TTTTGCAATGATTGGGGGAT+TGG - Chr5:85888224-85888243 MsG0580028755.01.T01:intron 40.0%
!! GTTTCACTATAGGCCTTGTT+TGG - Chr5:85890224-85890243 MsG0580028755.01.T01:intron 40.0%
AAAAGGAAACTGGCTGCAAC+AGG + Chr5:85889013-85889032 None:intergenic 45.0%
AAAGGAAACTGGCTGCAACA+GGG + Chr5:85889012-85889031 None:intergenic 45.0%
ACTGACGTGTCATAATGTGG+TGG - Chr5:85889540-85889559 MsG0580028755.01.T01:CDS 45.0%
AGCACGAACAACATCTCCAA+TGG + Chr5:85890536-85890555 None:intergenic 45.0%
AGCAGATTCAAACCGCGAAA+AGG + Chr5:85889030-85889049 None:intergenic 45.0%
ATCCAAATCCACAAAGGTCG+GGG - Chr5:85889715-85889734 MsG0580028755.01.T01:CDS 45.0%
ATGATTGGGGGATTGGATTC+TGG - Chr5:85888231-85888250 MsG0580028755.01.T01:intron 45.0%
CAGTATTGCAAGTGCGCATA+TGG - Chr5:85890379-85890398 MsG0580028755.01.T01:intron 45.0%
CATAATATCCCCGACCTTTG+TGG + Chr5:85889726-85889745 None:intergenic 45.0%
CATCCAAATCCACAAAGGTC+GGG - Chr5:85889714-85889733 MsG0580028755.01.T01:CDS 45.0%
CTCGCAGAACTTTGGTATCA+GGG + Chr5:85890076-85890095 None:intergenic 45.0%
CTGTATGCAAAGTTCAGCAG+TGG - Chr5:85889911-85889930 MsG0580028755.01.T01:intron 45.0%
GTAGAATGGAATGGTTGAGG+TGG + Chr5:85887715-85887734 None:intergenic 45.0%
TCAAACCGCGAAAAGGAAAC+TGG + Chr5:85889023-85889042 None:intergenic 45.0%
TCGCAGAACTTTGGTATCAG+GGG + Chr5:85890075-85890094 None:intergenic 45.0%
TGCGCACTTGCAATACTGTT+TGG + Chr5:85890377-85890396 None:intergenic 45.0%
! CACGTCAGTTAATGGATCTC+AGG + Chr5:85889529-85889548 None:intergenic 45.0%
! TCGCAATCCTCCTTGCATTT+TGG + Chr5:85888925-85888944 None:intergenic 45.0%
AAGGAAACTGGCTGCAACAG+GGG + Chr5:85889011-85889030 None:intergenic 50.0%
AGATGTTGTTCGTGCTGTCG+TGG - Chr5:85890540-85890559 MsG0580028755.01.T01:intron 50.0%
AGTGAGAGAGAGTGGCTTTC+GGG + Chr5:85887737-85887756 None:intergenic 50.0%
ATCCCCGACCTTTGTGGATT+TGG + Chr5:85889720-85889739 None:intergenic 50.0%
CATGTGGGGTTTGTGATGTG+TGG - Chr5:85888087-85888106 MsG0580028755.01.T01:CDS 50.0%
CGGGGATATTATGACCGAAG+AGG - Chr5:85889733-85889752 MsG0580028755.01.T01:CDS 50.0%
CGGGTAGAATGGAATGGTTG+AGG + Chr5:85887718-85887737 None:intergenic 50.0%
CTGAGGAAGATCATCGTGCA+GGG - Chr5:85889686-85889705 MsG0580028755.01.T01:CDS 50.0%
CTGCATTGCTCGCAGAACTT+TGG + Chr5:85890084-85890103 None:intergenic 50.0%
GACGTGTCATAATGTGGTGG+AGG - Chr5:85889543-85889562 MsG0580028755.01.T01:CDS 50.0%
GAGTGAGAGAGAGTGGCTTT+CGG + Chr5:85887738-85887757 None:intergenic 50.0%
GATGTTGTTCGTGCTGTCGT+GGG - Chr5:85890541-85890560 MsG0580028755.01.T01:intron 50.0%
GCTCGCAGAACTTTGGTATC+AGG + Chr5:85890077-85890096 None:intergenic 50.0%
GGGGAGGAGAAATCAATTGC+TGG - Chr5:85889318-85889337 MsG0580028755.01.T01:intron 50.0%
GGGTTTGTGATGTGTGGTGA+AGG - Chr5:85888093-85888112 MsG0580028755.01.T01:CDS 50.0%
TGACAAGGAAATGCTCGGCA+TGG - Chr5:85890507-85890526 MsG0580028755.01.T01:intron 50.0%
TGCTACACCTGCTCGTATTG+AGG - Chr5:85889046-85889065 MsG0580028755.01.T01:intron 50.0%
TGGACAGCCAAAATGCAAGG+AGG - Chr5:85888915-85888934 MsG0580028755.01.T01:intron 50.0%
! AGAGTGGCTTTCGGGTAGAA+TGG + Chr5:85887729-85887748 None:intergenic 50.0%
! AGTTAGCTTGAGTGCCCTCA+TGG + Chr5:85890652-85890671 None:intergenic 50.0%
! CTTTGGTGGTCGTGAAACCA+GGG - Chr5:85889298-85889317 MsG0580028755.01.T01:intron 50.0%
! GCAATTGATTTCTCCTCCCC+TGG + Chr5:85889318-85889337 None:intergenic 50.0%
! GGCTTTCGGGTAGAATGGAA+TGG + Chr5:85887724-85887743 None:intergenic 50.0%
! GTTAGCTTGAGTGCCCTCAT+GGG + Chr5:85890651-85890670 None:intergenic 50.0%
! TGCAGCCAGTTTCCTTTTCG+CGG - Chr5:85889015-85889034 MsG0580028755.01.T01:intron 50.0%
! TTTGGTGGTCGTGAAACCAG+GGG - Chr5:85889299-85889318 MsG0580028755.01.T01:intron 50.0%
!! GGAAAAGGTAAAGGCGCTGA+TGG - Chr5:85889110-85889129 MsG0580028755.01.T01:intron 50.0%
ATCTGTGGTGCACCAAAGCC+AGG + Chr5:85889138-85889157 None:intergenic 55.0%
ATGCTCGGCATGGTGTCCAT+TGG - Chr5:85890517-85890536 MsG0580028755.01.T01:intron 55.0%
CCACTCTCTCTCACTCTCTC+TGG - Chr5:85887742-85887761 MsG0580028755.01.T01:three_prime_UTR 55.0%
CCAGAGAGAGTGAGAGAGAG+TGG + Chr5:85887745-85887764 None:intergenic 55.0%
CCATGCTCCTCAATACGAGC+AGG + Chr5:85889056-85889075 None:intergenic 55.0%
CCTGAGGAAGATCATCGTGC+AGG - Chr5:85889685-85889704 MsG0580028755.01.T01:CDS 55.0%
CCTGCACGATGATCTTCCTC+AGG + Chr5:85889688-85889707 None:intergenic 55.0%
CCTGCTCGTATTGAGGAGCA+TGG - Chr5:85889053-85889072 MsG0580028755.01.T01:intron 55.0%
CGAGCAATGCAGCTGATGAC+TGG - Chr5:85890092-85890111 MsG0580028755.01.T01:intron 55.0%
GAGGTGGTGAGTGAGTGAGA+GGG + Chr5:85887699-85887718 None:intergenic 55.0%
GCAACAGGGGATTCACCATG+CGG + Chr5:85888998-85889017 None:intergenic 55.0%
TGAGGTGGTGAGTGAGTGAG+AGG + Chr5:85887700-85887719 None:intergenic 55.0%
! GCTTTGGTGGTCGTGAAACC+AGG - Chr5:85889297-85889316 MsG0580028755.01.T01:intron 55.0%
! TGTTTTGCAACGGGTCCGCA+TGG - Chr5:85888980-85888999 MsG0580028755.01.T01:CDS 55.0%
!! GTAAAGGCGCTGATGGTTCC+TGG - Chr5:85889117-85889136 MsG0580028755.01.T01:intron 55.0%
CAGCGATGCCCCTGCCAAAT+TGG - Chr5:85889865-85889884 MsG0580028755.01.T01:intron 60.0%
! GCGCTGATGGTTCCTGGCTT+TGG - Chr5:85889123-85889142 MsG0580028755.01.T01:intron 60.0%
GGTGGTCGTGAAACCAGGGG+AGG - Chr5:85889302-85889321 MsG0580028755.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 85887600 85891080 85887600 ID=MsG0580028755.01;Name=MsG0580028755.01
Chr5 mRNA 85887600 85891080 85887600 ID=MsG0580028755.01.T01;Parent=MsG0580028755.01;Name=MsG0580028755.01.T01;_AED=0.49;_eAED=0.49;_QI=28|1|1|1|0.8|0.66|6|462|252
Chr5 exon 85887600 85888204 85887600 ID=MsG0580028755.01.T01:exon:33486;Parent=MsG0580028755.01.T01
Chr5 exon 85888567 85888627 85888567 ID=MsG0580028755.01.T01:exon:33485;Parent=MsG0580028755.01.T01
Chr5 exon 85888926 85888999 85888926 ID=MsG0580028755.01.T01:exon:33484;Parent=MsG0580028755.01.T01
Chr5 exon 85889323 85889404 85889323 ID=MsG0580028755.01.T01:exon:33483;Parent=MsG0580028755.01.T01
Chr5 exon 85889496 85889758 85889496 ID=MsG0580028755.01.T01:exon:33482;Parent=MsG0580028755.01.T01
Chr5 exon 85890917 85891080 85890917 ID=MsG0580028755.01.T01:exon:33481;Parent=MsG0580028755.01.T01
Chr5 five_prime_UTR 85891053 85891080 85891053 ID=MsG0580028755.01.T01:five_prime_utr;Parent=MsG0580028755.01.T01
Chr5 CDS 85890917 85891052 85890917 ID=MsG0580028755.01.T01:cds;Parent=MsG0580028755.01.T01
Chr5 CDS 85889496 85889758 85889496 ID=MsG0580028755.01.T01:cds;Parent=MsG0580028755.01.T01
Chr5 CDS 85889323 85889404 85889323 ID=MsG0580028755.01.T01:cds;Parent=MsG0580028755.01.T01
Chr5 CDS 85888926 85888999 85888926 ID=MsG0580028755.01.T01:cds;Parent=MsG0580028755.01.T01
Chr5 CDS 85888567 85888627 85888567 ID=MsG0580028755.01.T01:cds;Parent=MsG0580028755.01.T01
Chr5 CDS 85888062 85888204 85888062 ID=MsG0580028755.01.T01:cds;Parent=MsG0580028755.01.T01
Chr5 three_prime_UTR 85887600 85888061 85887600 ID=MsG0580028755.01.T01:three_prime_utr;Parent=MsG0580028755.01.T01
Gene Sequence

>MsG0580028755.01.T01

ATGTACTATAGACAAGGCCCACTTCACAATTTTGTAAGATTTGAGGAACAAGTCAAGTGTTCTTGTCACCCTCTCACTCACTCACCACCTCAACCATTCCATTCTACCCGAAAGCCACTCTCTCTCACTCTCTCTGCCAAAATGCAAGGAGGATTGCGATCATTTTTGTCAAACGGGAACGTCATTAAAAATGCTGTTTTGCAACGGGTCCGCATGGTGAATCCCCTGTTGCAGCCAGTTTCCTTTTCGCGGTTTGAATCTGCTACACCTGCTCGTATTGAGGAGCATGGTTTCGAGAGCACCACAATTTCAGACATCTTGCAAGGAAAAGGTAAAGGCGCTGATGGTTCCTGGCTTTGGTGCACCACAGATGACACTGTTTATGATGCTGTCAAATCGATGACACAAAACAATGTTGGTGCTTTGGTGGTCGTGAAACCAGGGGAGGAGAAATCAATTGCTGGAATTATAACAGAAAGAGATTACCTGAGGAAGATCATCGTGCAGGGAAGATCATCCAAATCCACAAAGGTCGGGGATATTATGACCGAAGAGAACAAACTTATCACAGTCACCCCTGATACCAAAGTTCTGCGAGCAATGCAGCTGATGACTGATAACAGGATCAGACACATCCCTGTCATAAATGACAAGGAAATGCTCGGCATGGTGTCCATTGGAGATGTTGTTCGTGCTGTCGTGGGCGAGCATCGTCAAGAGCTTGACCGCTTGAATGCTTACATACAAGGGGGATATTAA
Protein sequence

>MsG0580028755.01.T01

MYYRQGPLHNFVRFEEQVKCSCHPLTHSPPQPFHSTRKPLSLTLSAKMQGGLRSFLSNGNVIKNAVLQRVRMVNPLLQPVSFSRFESATPARIEEHGFESTTISDILQGKGKGADGSWLWCTTDDTVYDAVKSMTQNNVGALVVVKPGEEKSIAGIITERDYLRKIIVQGRSSKSTKVGDIMTEENKLITVTPDTKVLRAMQLMTDNRIRHIPVINDKEMLGMVSIGDVVRAVVGEHRQELDRLNAYIQGGY*