AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003474.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003474.01.T01 MTR_1g062770 96.552 203 7 0 1 203 1 203 2.53e-142 395
MsG0180003474.01.T01 MTR_2g048680 35.000 120 71 2 64 183 94 206 4.08e-12 63.2
MsG0180003474.01.T01 MTR_3g009280 38.525 122 65 4 43 163 230 342 5.16e-12 63.9
MsG0180003474.01.T01 MTR_2g048680 34.167 120 72 1 64 183 174 286 8.95e-12 63.2
MsG0180003474.01.T01 MTR_8g056020 31.724 145 85 3 33 171 219 355 1.61e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003474.01.T01 AT3G09890 61.307 199 75 2 1 197 1 199 6.68e-78 232
MsG0180003474.01.T01 AT3G09890 60.135 148 57 2 1 146 1 148 1.39e-51 164
MsG0180003474.01.T01 AT4G19150 30.556 144 93 1 40 183 19 155 4.51e-14 68.9
MsG0180003474.01.T01 AT5G40160 31.667 180 107 5 12 183 119 290 8.17e-13 66.2
MsG0180003474.01.T01 AT2G17390 36.364 121 69 3 43 163 226 338 2.64e-12 65.1
MsG0180003474.01.T01 AT2G17390 33.333 111 67 1 73 183 222 325 3.33e-11 61.6
MsG0180003474.01.T01 AT4G35450 35.537 121 70 4 43 163 224 336 2.72e-12 65.1
MsG0180003474.01.T01 AT4G35450 35.537 121 70 4 43 163 224 336 2.72e-12 65.1
MsG0180003474.01.T01 AT4G35450 35.537 121 70 4 43 163 224 336 2.72e-12 65.1
MsG0180003474.01.T01 AT4G35450 35.537 121 70 4 43 163 232 344 2.99e-12 64.7
MsG0180003474.01.T01 AT4G35450 35.537 121 70 4 43 163 186 298 3.28e-12 64.3
MsG0180003474.01.T01 AT5G40160 32.143 168 98 5 12 171 119 278 4.07e-12 63.9
MsG0180003474.01.T01 AT2G03430 30.968 155 94 4 32 180 5 152 6.74e-12 62.8
MsG0180003474.01.T01 AT4G19150 32.110 109 67 1 75 183 31 132 4.43e-11 60.5

Find 81 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTGGTCATTTGAAATAT+TGG 0.218911 1:-62823749 MsG0180003474.01.T01:three_prime_UTR
CTCTGCCACTCTTCTCAAAT+TGG 0.257322 1:+62826232 None:intergenic
TTCAAACCATTGCGTCTTCC+TGG 0.282234 1:+62826154 None:intergenic
CTCTTCTCAAATTGGCTTTA+CGG 0.295298 1:+62826240 None:intergenic
CTTGCATCTGTGCCAGGTTC+AGG 0.300371 1:+62823991 None:intergenic
TCCACAAATCAAAACTTATA+TGG 0.302275 1:-62824104 MsG0180003474.01.T01:CDS
TACAGATTGGTCTATCAAAA+TGG 0.340793 1:+62823890 None:intergenic
CTTCATCTGACCTGTTTGTA+TGG 0.360852 1:-62825113 MsG0180003474.01.T01:CDS
ACCACAACAATGGCGGTTCC+AGG 0.389804 1:-62826172 MsG0180003474.01.T01:exon
GTTGAAGCGGCGGCAGAGAT+AGG 0.398168 1:-62826200 MsG0180003474.01.T01:five_prime_UTR
ATTAGATTGTTGCTGTCTAA+TGG 0.400508 1:-62824134 MsG0180003474.01.T01:CDS
CCAGGTTCAGGTAACTCTGC+AGG 0.402815 1:+62824003 None:intergenic
AACGCCCTCCGCCTCGCTCT+CGG 0.412363 1:-62826013 MsG0180003474.01.T01:intron
GAGTACCGAGAGCGAGGCGG+AGG 0.430564 1:+62826008 None:intergenic
AGCCTCCTTGCATCTGTGCC+AGG 0.449249 1:+62823985 None:intergenic
TAGCTCTGTTAAGTAGAAGT+TGG 0.455736 1:+62824797 None:intergenic
GTCACCGTGTGTTGCGGCAT+GGG 0.457918 1:+62826039 None:intergenic
GAGGATGCAGGGGTCAGGTT+AGG 0.461107 1:-62823793 MsG0180003474.01.T01:three_prime_UTR
GAAGAATCAAATGGTCTCTT+CGG 0.464532 1:-62826118 MsG0180003474.01.T01:CDS
CACTTCCTCGATTCTAAACT+AGG 0.471937 1:+62823848 None:intergenic
CCTTTACACGATGCTTGTGC+AGG 0.483914 1:-62824929 MsG0180003474.01.T01:CDS
TGATGAACCGGTGGAGGATG+GGG 0.487017 1:-62825144 MsG0180003474.01.T01:CDS
ACTAGAAAGAGGAGCCAACT+TGG 0.490839 1:-62824978 MsG0180003474.01.T01:CDS
AAAATGAGGATGCAGGGGTC+AGG 0.493583 1:-62823798 MsG0180003474.01.T01:three_prime_UTR
TGGTCATTTCGAATGCGCTC+AGG 0.500683 1:-62825093 MsG0180003474.01.T01:intron
AATGGTCTCTTCGGAGAAGA+AGG 0.510479 1:-62826109 MsG0180003474.01.T01:CDS
TCCTGGAACCGCCATTGTTG+TGG 0.510708 1:+62826171 None:intergenic
TTGATGAACCGGTGGAGGAT+GGG 0.512299 1:-62825145 MsG0180003474.01.T01:CDS
TTCGAAATGACCATACAAAC+AGG 0.512636 1:+62825103 None:intergenic
CGTGTGTTGCGGCATGGGAG+AGG 0.523848 1:+62826044 None:intergenic
TTGGAGGCTACAGATGAAGA+TGG 0.525829 1:-62824959 MsG0180003474.01.T01:CDS
TGGAGGCTACAGATGAAGAT+GGG 0.526662 1:-62824958 MsG0180003474.01.T01:CDS
CAGGTTCAGGTAACTCTGCA+GGG 0.527694 1:+62824004 None:intergenic
ATTGATGAACCGGTGGAGGA+TGG 0.527986 1:-62825146 MsG0180003474.01.T01:CDS
CTCTCCCATGCCGCAACACA+CGG 0.528668 1:-62826043 MsG0180003474.01.T01:CDS
GAAGACGATGAAGAATCAAA+TGG 0.533259 1:-62826127 MsG0180003474.01.T01:CDS
GAGTGGTAGTATTGATGAAC+CGG 0.535008 1:-62825156 MsG0180003474.01.T01:CDS
CCTCTTGCAGCATGATGAAG+AGG 0.537041 1:+62824173 None:intergenic
GAATATACAGTGATACAGAT+TGG 0.537149 1:+62823877 None:intergenic
CCTGCAGAGTTACCTGAACC+TGG 0.542445 1:-62824003 MsG0180003474.01.T01:CDS
TATTGGAATTACTCTATAGC+AGG 0.542653 1:-62823732 MsG0180003474.01.T01:three_prime_UTR
CCTGCACAAGCATCGTGTAA+AGG 0.546613 1:+62824929 None:intergenic
AATGCTATATTAGATTTCCA+TGG 0.546731 1:+62823942 None:intergenic
TTGCAGCTGCTACTAGAAAG+AGG 0.551764 1:-62824989 MsG0180003474.01.T01:intron
TTCATCTGTAGCCTCCAAGT+TGG 0.553926 1:+62824964 None:intergenic
CTCTTCATCATGCTGCAAGA+GGG 0.557025 1:-62824172 MsG0180003474.01.T01:CDS
GGTCACCGTGTGTTGCGGCA+TGG 0.558536 1:+62826038 None:intergenic
GGGGTCAGGTTAGGACGACT+TGG 0.562916 1:-62823784 MsG0180003474.01.T01:three_prime_UTR
CCTCTTCATCATGCTGCAAG+AGG 0.568776 1:-62824173 MsG0180003474.01.T01:CDS
GTAGTTTAAAGTTGAAGCGG+CGG 0.573044 1:-62826210 MsG0180003474.01.T01:five_prime_UTR
AAATCAAAACTTATATGGAA+AGG 0.581582 1:-62824099 MsG0180003474.01.T01:intron
TTACACGATGCTTGTGCAGG+AGG 0.583004 1:-62824926 MsG0180003474.01.T01:intron
GAGAGAGAGTACCGAGAGCG+AGG 0.583023 1:+62826002 None:intergenic
GCAGTATCCCCATCCTCCAC+CGG 0.583111 1:+62825137 None:intergenic
GTTGAGGTCACCGTGTGTTG+CGG 0.587853 1:+62826033 None:intergenic
TCTTCATCATGCTGCAAGAG+GGG 0.593759 1:-62824171 MsG0180003474.01.T01:CDS
GCTTGAATCAGTTGATTCTG+AGG 0.595184 1:-62824749 MsG0180003474.01.T01:CDS
TGATGCTGCTGCTACTGCCA+TGG 0.597475 1:-62823959 MsG0180003474.01.T01:CDS
AGTACCGAGAGCGAGGCGGA+GGG 0.600311 1:+62826009 None:intergenic
TGGGAGAGGTCACGAAGATG+AGG 0.605218 1:+62826058 None:intergenic
ACAATGCTGAGCATATAAGA+AGG 0.607273 1:-62824774 MsG0180003474.01.T01:CDS
TAAAGCCAATTTGAGAAGAG+TGG 0.610481 1:-62826237 MsG0180003474.01.T01:five_prime_UTR
GAGCGAGGCGGAGGGCGTTG+AGG 0.610700 1:+62826017 None:intergenic
AGAGTAGTTTAAAGTTGAAG+CGG 0.610763 1:-62826213 MsG0180003474.01.T01:five_prime_UTR
TTTCTCCTAGTTTAGAATCG+AGG 0.611282 1:-62823853 MsG0180003474.01.T01:three_prime_UTR
AGGTTCAGGTAACTCTGCAG+GGG 0.613740 1:+62824005 None:intergenic
CTGAACCTGGCACAGATGCA+AGG 0.614501 1:-62823990 MsG0180003474.01.T01:CDS
TAGTATTGATGAACCGGTGG+AGG 0.618172 1:-62825150 MsG0180003474.01.T01:CDS
GCGGTTCCAGGAAGACGCAA+TGG 0.619848 1:-62826160 MsG0180003474.01.T01:CDS
GAGAGGTCACGAAGATGAGG+AGG 0.622353 1:+62826061 None:intergenic
AGAAAGAGGAGCCAACTTGG+AGG 0.623979 1:-62824975 MsG0180003474.01.T01:CDS
AATCTAATATAGCATTAGCA+TGG 0.625742 1:-62823935 MsG0180003474.01.T01:exon
ATAATTGCAGATAACTTGAG+TGG 0.631826 1:-62825173 MsG0180003474.01.T01:intron
CTTGAATCAGTTGATTCTGA+GGG 0.651281 1:-62824748 MsG0180003474.01.T01:CDS
AGAGAGTACCGAGAGCGAGG+CGG 0.654197 1:+62826005 None:intergenic
CTAATATAGCATTAGCATGG+TGG 0.661514 1:-62823932 MsG0180003474.01.T01:exon
GGAGGCTACAGATGAAGATG+GGG 0.668284 1:-62824957 MsG0180003474.01.T01:CDS
TGGTAGTATTGATGAACCGG+TGG 0.685008 1:-62825153 MsG0180003474.01.T01:CDS
ATAGGAGAATACCACAACAA+TGG 0.686690 1:-62826182 MsG0180003474.01.T01:exon
GGAGAATACCACAACAATGG+CGG 0.703030 1:-62826179 MsG0180003474.01.T01:exon
AACCTGGCACAGATGCAAGG+AGG 0.718959 1:-62823987 MsG0180003474.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTTTTT+GGG - Chr1:62825640-62825659 MsG0180003474.01.T01:intron 0.0%
!!! ATTAAATTTTTAATTTGATG+CGG - Chr1:62824157-62824176 MsG0180003474.01.T01:CDS 10.0%
!!! GATAAATTGTTTTATTTTAA+TGG - Chr1:62824088-62824107 MsG0180003474.01.T01:intron 10.0%
!! TATATTTATAAATCCTGATA+TGG + Chr1:62825129-62825148 None:intergenic 15.0%
!!! AAATTGATTTTGATACATTT+AGG + Chr1:62825516-62825535 None:intergenic 15.0%
!!! AATTGATTTTGATACATTTA+GGG + Chr1:62825515-62825534 None:intergenic 15.0%
!!! CAATGTTTTAAAAATCAAAT+TGG + Chr1:62824637-62824656 None:intergenic 15.0%
!!! GATTTTATGAATTATAACTT+TGG - Chr1:62826122-62826141 MsG0180003474.01.T01:CDS 15.0%
!!! TTTTTTTTTTTTTTTGGGAA+GGG - Chr1:62825645-62825664 MsG0180003474.01.T01:intron 15.0%
!!! TTTTTTTTTTTTTTTTGGGA+AGG - Chr1:62825644-62825663 MsG0180003474.01.T01:intron 15.0%
!! AAATCAAAACTTATATGGAA+AGG - Chr1:62825849-62825868 MsG0180003474.01.T01:intron 20.0%
!! TTGAGTGTAAAAATAATGTT+TGG - Chr1:62825419-62825438 MsG0180003474.01.T01:intron 20.0%
!! TTTCATTTAATGTTGTATAG+TGG - Chr1:62824283-62824302 MsG0180003474.01.T01:intron 20.0%
!!! ACATTTGCTAACATAATTTT+TGG - Chr1:62825916-62825935 MsG0180003474.01.T01:intron 20.0%
!!! ATATATTTTGAAGTAGCTAT+AGG + Chr1:62824032-62824051 None:intergenic 20.0%
!!! ATTGATTTTGATACATTTAG+GGG + Chr1:62825514-62825533 None:intergenic 20.0%
!!! TAACTTTGGAATTAAAAATG+AGG - Chr1:62826136-62826155 MsG0180003474.01.T01:CDS 20.0%
!!! TATATTTTGAAGTAGCTATA+GGG + Chr1:62824031-62824050 None:intergenic 20.0%
!!! TTGGTTTGTTTTTATTTGTT+TGG - Chr1:62826183-62826202 MsG0180003474.01.T01:exon 20.0%
!!! TTTTTTTTTTTTTTGGGAAG+GGG - Chr1:62825646-62825665 MsG0180003474.01.T01:intron 20.0%
! AAAAAAAGAATGAATGAGCA+TGG + Chr1:62824889-62824908 None:intergenic 25.0%
! AATCTAATATAGCATTAGCA+TGG - Chr1:62826013-62826032 MsG0180003474.01.T01:intron 25.0%
! AATGCTATATTAGATTTCCA+TGG + Chr1:62826009-62826028 None:intergenic 25.0%
! AATTATGTTAGCAAATGTTG+AGG + Chr1:62825914-62825933 None:intergenic 25.0%
! AGAAATCATGTACATAAAGT+TGG + Chr1:62825717-62825736 None:intergenic 25.0%
! AGCATCATAAGAATTAAGAA+AGG + Chr1:62824741-62824760 None:intergenic 25.0%
! AGTGTAAAAATAATGTTTGG+AGG - Chr1:62825422-62825441 MsG0180003474.01.T01:intron 25.0%
! CCACTAATTATAACAATGTA+GGG - Chr1:62825591-62825610 MsG0180003474.01.T01:intron 25.0%
! GTGTAAAAATAATGTTTGGA+GGG - Chr1:62825423-62825442 MsG0180003474.01.T01:intron 25.0%
! TCCACAAATCAAAACTTATA+TGG - Chr1:62825844-62825863 MsG0180003474.01.T01:intron 25.0%
! TCCACTAATTATAACAATGT+AGG - Chr1:62825590-62825609 MsG0180003474.01.T01:intron 25.0%
! TTAATTAGAATAAACCCTAG+AGG - Chr1:62825466-62825485 MsG0180003474.01.T01:intron 25.0%
!! TGTTATAATTAGTGGATGTT+GGG + Chr1:62825586-62825605 None:intergenic 25.0%
!! TGTTTGGTCATTTGAAATAT+TGG - Chr1:62826199-62826218 MsG0180003474.01.T01:five_prime_UTR 25.0%
!! TTGTTATAATTAGTGGATGT+TGG + Chr1:62825587-62825606 None:intergenic 25.0%
!!! ATTTTAATGGCATTTGATTC+TGG - Chr1:62824101-62824120 MsG0180003474.01.T01:CDS 25.0%
!!! TCCATATAAGTTTTGATTTG+TGG + Chr1:62825848-62825867 None:intergenic 25.0%
!!! TGATGTTACTTTTCTTTGAA+TGG - Chr1:62825103-62825122 MsG0180003474.01.T01:CDS 25.0%
AATTAAAAATGAGGATGCAG+GGG - Chr1:62826145-62826164 MsG0180003474.01.T01:CDS 30.0%
ATAAAGTTGGAACAGGATTA+GGG + Chr1:62825704-62825723 None:intergenic 30.0%
ATAATTGCAGATAACTTGAG+TGG - Chr1:62824775-62824794 MsG0180003474.01.T01:CDS 30.0%
ATGTTGTCAAATATCAGCTA+TGG - Chr1:62824482-62824501 MsG0180003474.01.T01:intron 30.0%
ATTAGATTGTTGCTGTCTAA+TGG - Chr1:62825814-62825833 MsG0180003474.01.T01:intron 30.0%
ATTGAAATTGACAGTGCAAT+CGG - Chr1:62824230-62824249 MsG0180003474.01.T01:intron 30.0%
CAAGATATGGAGAACAAATA+AGG + Chr1:62825740-62825759 None:intergenic 30.0%
CCCTACATTGTTATAATTAG+TGG + Chr1:62825594-62825613 None:intergenic 30.0%
GAATATACAGTGATACAGAT+TGG + Chr1:62826074-62826093 None:intergenic 30.0%
GAATTAAAAATGAGGATGCA+GGG - Chr1:62826144-62826163 MsG0180003474.01.T01:CDS 30.0%
TATCATACTGGGAAGTTATT+GGG - Chr1:62824712-62824731 MsG0180003474.01.T01:intron 30.0%
TATTGGAATTACTCTATAGC+AGG - Chr1:62826216-62826235 MsG0180003474.01.T01:five_prime_UTR 30.0%
TTCAAATTCCACTAAGCTAT+AGG + Chr1:62824423-62824442 None:intergenic 30.0%
TTTCGTTCAATGTTGTATAG+TGG - Chr1:62824397-62824416 MsG0180003474.01.T01:intron 30.0%
! AGAGTAGTTTAAAGTTGAAG+CGG - Chr1:62823735-62823754 MsG0180003474.01.T01:three_prime_UTR 30.0%
! ATCAAATCAACGACATCTTT+TGG + Chr1:62824612-62824631 None:intergenic 30.0%
! TGCTATTTAGTACAAGATGT+TGG - Chr1:62824353-62824372 MsG0180003474.01.T01:intron 30.0%
! TTGACTTTGGCTTATTGAAT+AGG + Chr1:62825268-62825287 None:intergenic 30.0%
! TTTTAATCTGTAACATGTCG+TGG - Chr1:62824914-62824933 MsG0180003474.01.T01:intron 30.0%
!! TACAGATTGGTCTATCAAAA+TGG + Chr1:62826061-62826080 None:intergenic 30.0%
AACTTCCCAGTATGATAGTT+AGG + Chr1:62824709-62824728 None:intergenic 35.0%
ACAATGCTGAGCATATAAGA+AGG - Chr1:62825174-62825193 MsG0180003474.01.T01:intron 35.0%
AGACATCCTAACTATCATAC+TGG - Chr1:62824700-62824719 MsG0180003474.01.T01:intron 35.0%
ATAGGAGAATACCACAACAA+TGG - Chr1:62823766-62823785 MsG0180003474.01.T01:three_prime_UTR 35.0%
ATCTGCAATTATGCAACACA+GGG + Chr1:62824767-62824786 None:intergenic 35.0%
ATGTTGTATAGTGGCACTAT+AGG - Chr1:62824292-62824311 MsG0180003474.01.T01:intron 35.0%
ATTAGGGATTAGGATTTAGC+CGG + Chr1:62825688-62825707 None:intergenic 35.0%
CATAAAGTTGGAACAGGATT+AGG + Chr1:62825705-62825724 None:intergenic 35.0%
CATGTACATAAAGTTGGAAC+AGG + Chr1:62825711-62825730 None:intergenic 35.0%
CTAATATAGCATTAGCATGG+TGG - Chr1:62826016-62826035 MsG0180003474.01.T01:CDS 35.0%
CTATAGGGTTTAACAGAAAG+AGG + Chr1:62824016-62824035 None:intergenic 35.0%
CTATCATACTGGGAAGTTAT+TGG - Chr1:62824711-62824730 MsG0180003474.01.T01:intron 35.0%
GAAGAATCAAATGGTCTCTT+CGG - Chr1:62823830-62823849 MsG0180003474.01.T01:three_prime_UTR 35.0%
GAAGACGATGAAGAATCAAA+TGG - Chr1:62823821-62823840 MsG0180003474.01.T01:three_prime_UTR 35.0%
GACATCCTAACTATCATACT+GGG - Chr1:62824701-62824720 MsG0180003474.01.T01:intron 35.0%
GCGAAAATAGTCACAAGATA+TGG + Chr1:62825753-62825772 None:intergenic 35.0%
GGAATTAAAAATGAGGATGC+AGG - Chr1:62826143-62826162 MsG0180003474.01.T01:CDS 35.0%
TAAAGCCAATTTGAGAAGAG+TGG - Chr1:62823711-62823730 MsG0180003474.01.T01:three_prime_UTR 35.0%
TATCTGCAATTATGCAACAC+AGG + Chr1:62824768-62824787 None:intergenic 35.0%
TGTTGTATAGTGGCACTATA+GGG - Chr1:62824293-62824312 MsG0180003474.01.T01:intron 35.0%
TTCGAAATGACCATACAAAC+AGG + Chr1:62824848-62824867 None:intergenic 35.0%
TTTCTTTGAATGGCCATATC+AGG - Chr1:62825113-62825132 MsG0180003474.01.T01:CDS 35.0%
! CTTGAATCAGTTGATTCTGA+GGG - Chr1:62825200-62825219 MsG0180003474.01.T01:intron 35.0%
! TAGCTCTGTTAAGTAGAAGT+TGG + Chr1:62825154-62825173 None:intergenic 35.0%
! TAGGAACTCACACTTTTAGA+AGG + Chr1:62825249-62825268 None:intergenic 35.0%
! TGTACTAAAGAGCATATCAC+AGG + Chr1:62824460-62824479 None:intergenic 35.0%
! TTTCTCCTAGTTTAGAATCG+AGG - Chr1:62826095-62826114 MsG0180003474.01.T01:CDS 35.0%
!! CTCTTCTCAAATTGGCTTTA+CGG + Chr1:62823711-62823730 None:intergenic 35.0%
AATTGACAGTGCAATCGGTT+TGG - Chr1:62824235-62824254 MsG0180003474.01.T01:intron 40.0%
ACAATCTGTGATGACAATGC+CGG - Chr1:62824545-62824564 MsG0180003474.01.T01:intron 40.0%
AGAGAATTCTGTTTGCCTCT+AGG + Chr1:62825484-62825503 None:intergenic 40.0%
CAATCGAACTGAGACAAATC+CGG + Chr1:62824567-62824586 None:intergenic 40.0%
CACTTCCTCGATTCTAAACT+AGG + Chr1:62826103-62826122 None:intergenic 40.0%
CTTCATCTGACCTGTTTGTA+TGG - Chr1:62824835-62824854 MsG0180003474.01.T01:intron 40.0%
GAGAATTCTGTTTGCCTCTA+GGG + Chr1:62825483-62825502 None:intergenic 40.0%
GTTGTATAGTGGCACTATAG+GGG - Chr1:62824294-62824313 MsG0180003474.01.T01:intron 40.0%
TATAGTGGCCTATAGCTTAG+TGG - Chr1:62824412-62824431 MsG0180003474.01.T01:intron 40.0%
TGGATGTTGGGATTCAGTTT+GGG + Chr1:62825574-62825593 None:intergenic 40.0%
TTGGAACAGGATTAGGGATT+AGG + Chr1:62825698-62825717 None:intergenic 40.0%
! GCTTGAATCAGTTGATTCTG+AGG - Chr1:62825199-62825218 MsG0180003474.01.T01:intron 40.0%
! GTAGTTTAAAGTTGAAGCGG+CGG - Chr1:62823738-62823757 MsG0180003474.01.T01:three_prime_UTR 40.0%
! TCAACGACATCTTTTGGTCA+TGG + Chr1:62824606-62824625 None:intergenic 40.0%
!! GAGTGGTAGTATTGATGAAC+CGG - Chr1:62824792-62824811 MsG0180003474.01.T01:CDS 40.0%
AAGTCAATCCAACGACCTTC+TGG - Chr1:62825281-62825300 MsG0180003474.01.T01:intron 45.0%
AATGGTCTCTTCGGAGAAGA+AGG - Chr1:62823839-62823858 MsG0180003474.01.T01:three_prime_UTR 45.0%
ACTAGAAAGAGGAGCCAACT+TGG - Chr1:62824970-62824989 MsG0180003474.01.T01:CDS 45.0%
CGGATTTGTCTCAGTTCGAT+TGG - Chr1:62824565-62824584 MsG0180003474.01.T01:intron 45.0%
CTCTGCCACTCTTCTCAAAT+TGG + Chr1:62823719-62823738 None:intergenic 45.0%
CTCTTCATCATGCTGCAAGA+GGG - Chr1:62825776-62825795 MsG0180003474.01.T01:intron 45.0%
GGAGAATACCACAACAATGG+CGG - Chr1:62823769-62823788 MsG0180003474.01.T01:three_prime_UTR 45.0%
GGATGTTGGGATTCAGTTTG+GGG + Chr1:62825573-62825592 None:intergenic 45.0%
GGGATCAAATGAGTAGACAC+CGG - Chr1:62825666-62825685 MsG0180003474.01.T01:intron 45.0%
GTGGATGTTGGGATTCAGTT+TGG + Chr1:62825575-62825594 None:intergenic 45.0%
TCTTCATCATGCTGCAAGAG+GGG - Chr1:62825777-62825796 MsG0180003474.01.T01:intron 45.0%
TGGAGGCTACAGATGAAGAT+GGG - Chr1:62824990-62825009 MsG0180003474.01.T01:intron 45.0%
TTCAAACCATTGCGTCTTCC+TGG + Chr1:62823797-62823816 None:intergenic 45.0%
TTCATCTGTAGCCTCCAAGT+TGG + Chr1:62824987-62825006 None:intergenic 45.0%
TTGCAGCTGCTACTAGAAAG+AGG - Chr1:62824959-62824978 MsG0180003474.01.T01:CDS 45.0%
TTGGAGGCTACAGATGAAGA+TGG - Chr1:62824989-62825008 MsG0180003474.01.T01:intron 45.0%
! GAAGGTCGTTGGATTGACTT+TGG + Chr1:62825281-62825300 None:intergenic 45.0%
!! TAGTATTGATGAACCGGTGG+AGG - Chr1:62824798-62824817 MsG0180003474.01.T01:CDS 45.0%
!! TGGTAGTATTGATGAACCGG+TGG - Chr1:62824795-62824814 MsG0180003474.01.T01:CDS 45.0%
!!! AAACATTTAAAATAATAATT+AGG + Chr1:62824066-62824085 None:intergenic 5.0%
!!! AACATTTAAAATAATAATTA+GGG + Chr1:62824065-62824084 None:intergenic 5.0%
!!! GTTTTTTTTTTTTTTTTTTT+TGG - Chr1:62825639-62825658 MsG0180003474.01.T01:intron 5.0%
AAAATGAGGATGCAGGGGTC+AGG - Chr1:62826150-62826169 MsG0180003474.01.T01:CDS 50.0%
AGAAAGAGGAGCCAACTTGG+AGG - Chr1:62824973-62824992 MsG0180003474.01.T01:CDS 50.0%
AGAACCAACCACACACCAGA+AGG + Chr1:62825299-62825318 None:intergenic 50.0%
AGGTTCAGGTAACTCTGCAG+GGG + Chr1:62825946-62825965 None:intergenic 50.0%
ATAGTGGCACTATAGGGGAG+TGG - Chr1:62824299-62824318 MsG0180003474.01.T01:intron 50.0%
ATTGATGAACCGGTGGAGGA+TGG - Chr1:62824802-62824821 MsG0180003474.01.T01:CDS 50.0%
CAGGTTCAGGTAACTCTGCA+GGG + Chr1:62825947-62825966 None:intergenic 50.0%
CCTCTTCATCATGCTGCAAG+AGG - Chr1:62825775-62825794 MsG0180003474.01.T01:intron 50.0%
CCTCTTGCAGCATGATGAAG+AGG + Chr1:62825778-62825797 None:intergenic 50.0%
CCTTTACACGATGCTTGTGC+AGG - Chr1:62825019-62825038 MsG0180003474.01.T01:intron 50.0%
GGAGGCTACAGATGAAGATG+GGG - Chr1:62824991-62825010 MsG0180003474.01.T01:intron 50.0%
TGGTCATTTCGAATGCGCTC+AGG - Chr1:62824855-62824874 MsG0180003474.01.T01:intron 50.0%
TTACACGATGCTTGTGCAGG+AGG - Chr1:62825022-62825041 MsG0180003474.01.T01:intron 50.0%
TTGATGAACCGGTGGAGGAT+GGG - Chr1:62824803-62824822 MsG0180003474.01.T01:CDS 50.0%
! CCTGCACAAGCATCGTGTAA+AGG + Chr1:62825022-62825041 None:intergenic 50.0%
AACCTGGCACAGATGCAAGG+AGG - Chr1:62825961-62825980 MsG0180003474.01.T01:intron 55.0%
ACCACAACAATGGCGGTTCC+AGG - Chr1:62823776-62823795 MsG0180003474.01.T01:three_prime_UTR 55.0%
ACCACACACCAGAAGGTCGT+TGG + Chr1:62825292-62825311 None:intergenic 55.0%
ACGACCTTCTGGTGTGTGGT+TGG - Chr1:62825292-62825311 MsG0180003474.01.T01:intron 55.0%
CCAGGTTCAGGTAACTCTGC+AGG + Chr1:62825948-62825967 None:intergenic 55.0%
CCTGCAGAGTTACCTGAACC+TGG - Chr1:62825945-62825964 MsG0180003474.01.T01:intron 55.0%
CTGAACCTGGCACAGATGCA+AGG - Chr1:62825958-62825977 MsG0180003474.01.T01:intron 55.0%
CTTGCATCTGTGCCAGGTTC+AGG + Chr1:62825960-62825979 None:intergenic 55.0%
GAGAGGTCACGAAGATGAGG+AGG + Chr1:62823890-62823909 None:intergenic 55.0%
GTTGAGGTCACCGTGTGTTG+CGG + Chr1:62823918-62823937 None:intergenic 55.0%
TCCAACGACCTTCTGGTGTG+TGG - Chr1:62825288-62825307 MsG0180003474.01.T01:intron 55.0%
TCCTGGAACCGCCATTGTTG+TGG + Chr1:62823780-62823799 None:intergenic 55.0%
TGATGAACCGGTGGAGGATG+GGG - Chr1:62824804-62824823 MsG0180003474.01.T01:CDS 55.0%
TGATGCTGCTGCTACTGCCA+TGG - Chr1:62825989-62826008 MsG0180003474.01.T01:intron 55.0%
TGGGAGAGGTCACGAAGATG+AGG + Chr1:62823893-62823912 None:intergenic 55.0%
! GCTATGGCTCTGTTGCGTAG+CGG - Chr1:62824498-62824517 MsG0180003474.01.T01:intron 55.0%
AGCCTCCTTGCATCTGTGCC+AGG + Chr1:62825966-62825985 None:intergenic 60.0%
CTCTCCCATGCCGCAACACA+CGG - Chr1:62823905-62823924 MsG0180003474.01.T01:three_prime_UTR 60.0%
GAGGATGCAGGGGTCAGGTT+AGG - Chr1:62826155-62826174 MsG0180003474.01.T01:CDS 60.0%
GCAGTATCCCCATCCTCCAC+CGG + Chr1:62824814-62824833 None:intergenic 60.0%
GCGGTTCCAGGAAGACGCAA+TGG - Chr1:62823788-62823807 MsG0180003474.01.T01:three_prime_UTR 60.0%
GGGGTCAGGTTAGGACGACT+TGG - Chr1:62826164-62826183 MsG0180003474.01.T01:CDS 60.0%
GTCACCGTGTGTTGCGGCAT+GGG + Chr1:62823912-62823931 None:intergenic 60.0%
GTTGAAGCGGCGGCAGAGAT+AGG - Chr1:62823748-62823767 MsG0180003474.01.T01:three_prime_UTR 60.0%
!! AGAGAGTACCGAGAGCGAGG+CGG + Chr1:62823946-62823965 None:intergenic 60.0%
!! GAGAGAGAGTACCGAGAGCG+AGG + Chr1:62823949-62823968 None:intergenic 60.0%
CGTGTGTTGCGGCATGGGAG+AGG + Chr1:62823907-62823926 None:intergenic 65.0%
GGTCACCGTGTGTTGCGGCA+TGG + Chr1:62823913-62823932 None:intergenic 65.0%
! AGTACCGAGAGCGAGGCGGA+GGG + Chr1:62823942-62823961 None:intergenic 65.0%
AACGCCCTCCGCCTCGCTCT+CGG - Chr1:62823935-62823954 MsG0180003474.01.T01:exon 70.0%
!! GAGTACCGAGAGCGAGGCGG+AGG + Chr1:62823943-62823962 None:intergenic 70.0%
!! GAGCGAGGCGGAGGGCGTTG+AGG + Chr1:62823934-62823953 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 62823695 62826275 62823695 ID=MsG0180003474.01;Name=MsG0180003474.01
Chr1 mRNA 62823695 62826275 62823695 ID=MsG0180003474.01.T01;Parent=MsG0180003474.01;Name=MsG0180003474.01.T01;_AED=0.34;_eAED=0.34;_QI=90|1|1|1|1|1|6|256|203
Chr1 exon 62823695 62824028 62823695 ID=MsG0180003474.01.T01:exon:50004;Parent=MsG0180003474.01.T01
Chr1 exon 62824100 62824195 62824100 ID=MsG0180003474.01.T01:exon:50003;Parent=MsG0180003474.01.T01
Chr1 exon 62824741 62824835 62824741 ID=MsG0180003474.01.T01:exon:50002;Parent=MsG0180003474.01.T01
Chr1 exon 62824927 62825005 62824927 ID=MsG0180003474.01.T01:exon:50001;Parent=MsG0180003474.01.T01
Chr1 exon 62825094 62825185 62825094 ID=MsG0180003474.01.T01:exon:50000;Parent=MsG0180003474.01.T01
Chr1 exon 62826014 62826275 62826014 ID=MsG0180003474.01.T01:exon:49999;Parent=MsG0180003474.01.T01
Chr1 five_prime_UTR 62826186 62826275 62826186 ID=MsG0180003474.01.T01:five_prime_utr;Parent=MsG0180003474.01.T01
Chr1 CDS 62826014 62826185 62826014 ID=MsG0180003474.01.T01:cds;Parent=MsG0180003474.01.T01
Chr1 CDS 62825094 62825185 62825094 ID=MsG0180003474.01.T01:cds;Parent=MsG0180003474.01.T01
Chr1 CDS 62824927 62825005 62824927 ID=MsG0180003474.01.T01:cds;Parent=MsG0180003474.01.T01
Chr1 CDS 62824741 62824835 62824741 ID=MsG0180003474.01.T01:cds;Parent=MsG0180003474.01.T01
Chr1 CDS 62824100 62824195 62824100 ID=MsG0180003474.01.T01:cds;Parent=MsG0180003474.01.T01
Chr1 CDS 62823951 62824028 62823951 ID=MsG0180003474.01.T01:cds;Parent=MsG0180003474.01.T01
Chr1 three_prime_UTR 62823695 62823950 62823695 ID=MsG0180003474.01.T01:three_prime_utr;Parent=MsG0180003474.01.T01
Gene Sequence

>MsG0180003474.01.T01

ATGGCGGTTCCAGGAAGACGCAATGGTTTGAATGAAGAAGACGATGAAGAATCAAATGGTCTCTTCGGAGAAGAAGGTCTTGTTGCTGAAGAATCTGATACTCCTCCTCATCTTCGTGACCTCTCCCATGCCGCAACACACGGTGACCTCAACGCCCTCCGCCTCGCTCTCGATAACTTGAGTGGTAGTATTGATGAACCGGTGGAGGATGGGGATACTGCTCTTCATCTGACCTGTTTGTATGGTCATTTCGAATGCGCTCAGCTGCTACTAGAAAGAGGAGCCAACTTGGAGGCTACAGATGAAGATGGGGCAATTCCTTTACACGATGCTTGTGCAGGAGGATTTATAAATATAGTCCAACTTCTACTTAACAGAGCTAACAATGCTGAGCATATAAGAAGGATGCTTGAATCAGTTGATTCTGAGGGTGATACTCCTCTTCATCATGCTGCAAGAGGGGAGCATGCTGATGTAATTAGATTGTTGCTGTCTAATGGTGCATCATCCACAAATCAAAACTTATATGGAAAGACCCCTGCAGAGTTACCTGAACCTGGCACAGATGCAAGGAGGCTGTTTGATGCTGCTGCTACTGCCATGGAAATCTAA
Protein sequence

>MsG0180003474.01.T01

MAVPGRRNGLNEEDDEESNGLFGEEGLVAEESDTPPHLRDLSHAATHGDLNALRLALDNLSGSIDEPVEDGDTALHLTCLYGHFECAQLLLERGANLEATDEDGAIPLHDACAGGFINIVQLLLNRANNAEHIRRMLESVDSEGDTPLHHAARGEHADVIRLLLSNGASSTNQNLYGKTPAELPEPGTDARRLFDAAATAMEI*