Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029292.01.T01 | XP_003616749.2 | 85.039 | 127 | 16 | 1 | 1 | 124 | 1 | 127 | 5.78E-70 | 216 |
| MsG0580029292.01.T02 | XP_003616749.2 | 83.784 | 111 | 15 | 1 | 1 | 108 | 17 | 127 | 3.44E-58 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029292.01.T01 | O49453 | 42.336 | 137 | 53 | 4 | 5 | 122 | 20 | 149 | 2.19E-22 | 89 |
| MsG0580029292.01.T02 | O49453 | 49.398 | 83 | 40 | 2 | 24 | 106 | 69 | 149 | 6.13E-18 | 77 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029292.01.T01 | G7KH19 | 85.039 | 127 | 16 | 1 | 1 | 124 | 1 | 127 | 2.76e-70 | 216 |
| MsG0580029292.01.T02 | G7KH19 | 83.784 | 111 | 15 | 1 | 1 | 108 | 17 | 127 | 1.64e-58 | 185 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480018664.01 | MsG0580029292.01 | 0.807201 | 5.674301e-50 | 3.141091e-47 |
| MsG0580026222.01 | MsG0580029292.01 | 0.809732 | 1.636230e-50 | 9.682494e-48 |
| MsG0580027332.01 | MsG0580029292.01 | 0.806014 | 1.010450e-49 | 5.425613e-47 |
| MsG0580029292.01 | MsG0580029381.01 | 0.803679 | 3.107054e-49 | 1.571025e-46 |
| MsG0580029292.01 | MsG0780037606.01 | 0.813770 | 2.163617e-51 | 1.425655e-48 |
| MsG0580029292.01 | MsG0780039282.01 | 0.818175 | 2.248014e-52 | 1.670567e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029292.01.T01 | MTR_5g083870 | 85.039 | 127 | 16 | 1 | 1 | 124 | 1 | 127 | 7.00e-74 | 216 |
| MsG0580029292.01.T01 | MTR_5g083900 | 70.732 | 123 | 35 | 1 | 2 | 124 | 108 | 229 | 1.35e-60 | 186 |
| MsG0580029292.01.T01 | MTR_8g064590 | 50.794 | 126 | 52 | 3 | 5 | 122 | 11 | 134 | 6.12e-31 | 107 |
| MsG0580029292.01.T01 | MTR_5g034290 | 48.000 | 125 | 55 | 3 | 5 | 121 | 11 | 133 | 7.00e-25 | 92.0 |
| MsG0580029292.01.T01 | MTR_5g034290 | 48.000 | 125 | 55 | 3 | 5 | 121 | 44 | 166 | 2.26e-24 | 91.7 |
| MsG0580029292.01.T01 | MTR_2g090635 | 41.538 | 130 | 62 | 4 | 5 | 122 | 10 | 137 | 3.14e-22 | 85.5 |
| MsG0580029292.01.T01 | MTR_2g014360 | 39.695 | 131 | 63 | 3 | 5 | 121 | 20 | 148 | 8.20e-22 | 84.7 |
| MsG0580029292.01.T01 | MTR_6g060020 | 52.500 | 80 | 36 | 2 | 43 | 122 | 20 | 97 | 1.74e-16 | 69.7 |
| MsG0580029292.01.T02 | MTR_5g083870 | 83.784 | 111 | 15 | 1 | 1 | 108 | 17 | 127 | 4.16e-62 | 185 |
| MsG0580029292.01.T02 | MTR_5g083900 | 71.296 | 108 | 30 | 1 | 1 | 108 | 123 | 229 | 4.30e-52 | 163 |
| MsG0580029292.01.T02 | MTR_8g064590 | 52.294 | 109 | 42 | 3 | 6 | 106 | 28 | 134 | 2.47e-26 | 95.1 |
| MsG0580029292.01.T02 | MTR_5g034290 | 50.000 | 106 | 43 | 3 | 8 | 105 | 30 | 133 | 3.49e-21 | 82.0 |
| MsG0580029292.01.T02 | MTR_5g034290 | 50.000 | 106 | 43 | 3 | 8 | 105 | 63 | 166 | 8.01e-21 | 82.0 |
| MsG0580029292.01.T02 | MTR_2g014360 | 50.000 | 82 | 39 | 2 | 24 | 105 | 69 | 148 | 7.55e-19 | 76.6 |
| MsG0580029292.01.T02 | MTR_2g090635 | 41.593 | 113 | 52 | 4 | 6 | 106 | 27 | 137 | 2.78e-17 | 72.4 |
| MsG0580029292.01.T02 | MTR_6g060020 | 52.500 | 80 | 36 | 2 | 27 | 106 | 20 | 97 | 1.21e-16 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029292.01.T01 | AT1G10590 | 46.875 | 128 | 57 | 2 | 5 | 122 | 11 | 137 | 3.12e-29 | 103 |
| MsG0580029292.01.T01 | AT1G10590 | 46.875 | 128 | 57 | 2 | 5 | 122 | 11 | 137 | 3.12e-29 | 103 |
| MsG0580029292.01.T01 | AT1G10590 | 46.875 | 128 | 57 | 2 | 5 | 122 | 25 | 151 | 4.06e-29 | 103 |
| MsG0580029292.01.T01 | AT1G23750 | 46.032 | 126 | 58 | 3 | 5 | 122 | 12 | 135 | 1.83e-28 | 101 |
| MsG0580029292.01.T01 | AT2G33845 | 44.882 | 127 | 59 | 3 | 5 | 122 | 55 | 179 | 1.04e-23 | 90.5 |
| MsG0580029292.01.T01 | AT4G28440 | 42.336 | 137 | 53 | 4 | 5 | 122 | 20 | 149 | 2.23e-23 | 89.0 |
| MsG0580029292.01.T01 | AT1G23750 | 52.326 | 86 | 39 | 2 | 37 | 122 | 15 | 98 | 1.40e-22 | 85.5 |
| MsG0580029292.01.T02 | AT1G23750 | 46.789 | 109 | 48 | 3 | 6 | 106 | 29 | 135 | 8.83e-24 | 89.0 |
| MsG0580029292.01.T02 | AT1G10590 | 46.847 | 111 | 48 | 2 | 6 | 106 | 28 | 137 | 2.12e-23 | 87.8 |
| MsG0580029292.01.T02 | AT1G10590 | 46.847 | 111 | 48 | 2 | 6 | 106 | 28 | 137 | 2.12e-23 | 87.8 |
| MsG0580029292.01.T02 | AT1G10590 | 46.847 | 111 | 48 | 2 | 6 | 106 | 42 | 151 | 2.94e-23 | 87.8 |
| MsG0580029292.01.T02 | AT1G23750 | 50.000 | 92 | 44 | 2 | 15 | 106 | 9 | 98 | 9.38e-23 | 85.1 |
| MsG0580029292.01.T02 | AT2G33845 | 45.455 | 110 | 49 | 3 | 6 | 106 | 72 | 179 | 5.81e-19 | 77.8 |
| MsG0580029292.01.T02 | AT4G28440 | 49.398 | 83 | 40 | 2 | 24 | 106 | 69 | 149 | 6.25e-19 | 77.0 |
| MsG0580029292.01.T02 | AT1G03810 | 37.975 | 79 | 47 | 2 | 24 | 102 | 63 | 139 | 2.56e-11 | 57.0 |
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGCCTTGCGCACTATAA+TGG | 0.387382 | 5:-94824283 | None:intergenic |
| GCAAGGATACTTATGTATAA+AGG | 0.392913 | 5:+94824300 | MsG0580029292.01.T01:CDS |
| AAGTAGACGTCTTCCATCTC+AGG | 0.420369 | 5:-94824117 | None:intergenic |
| TGTCAAGGATGTGTCTCATA+AGG | 0.426882 | 5:+94824158 | MsG0580029292.01.T01:CDS |
| TAGTCAAAGAAGATTGTAAC+TGG | 0.434881 | 5:+94824388 | MsG0580029292.01.T01:CDS |
| ACAATCTTCTTTGACTATGA+AGG | 0.452452 | 5:-94824382 | None:intergenic |
| TCTCTCGTAGGCGACGAAAC+TGG | 0.463685 | 5:+94824201 | MsG0580029292.01.T01:CDS |
| GTCAAGGATGTGTCTCATAA+GGG | 0.515843 | 5:+94824159 | MsG0580029292.01.T01:CDS |
| TAAAATCAATCGAATCAAAG+AGG | 0.521606 | 5:+94824254 | MsG0580029292.01.T01:CDS |
| GCATGACCTACGCCCTGAGA+TGG | 0.542133 | 5:+94824104 | MsG0580029292.01.T01:CDS |
| GTCTCATAAGGGTAAGATAC+CGG | 0.550036 | 5:+94824170 | MsG0580029292.01.T01:CDS |
| TCTTTGACTATGAAGGCAGC+AGG | 0.557152 | 5:-94824375 | None:intergenic |
| CCATTATAGTGCGCAAGGCA+AGG | 0.557600 | 5:+94824283 | MsG0580029292.01.T01:CDS |
| TCTAACACCTGTGTTCATCA+AGG | 0.568192 | 5:+94824080 | MsG0580029292.01.T01:CDS |
| ATCAACCATTATAGTGCGCA+AGG | 0.581031 | 5:+94824278 | MsG0580029292.01.T01:CDS |
| ATCTTCATATTCAGAGCGAT+CGG | 0.585259 | 5:+94824225 | MsG0580029292.01.T01:CDS |
| AAAATCAATCGAATCAAAGA+GGG | 0.597492 | 5:+94824255 | MsG0580029292.01.T01:CDS |
| CGTCTTCCATCTCAGGGCGT+AGG | 0.606368 | 5:-94824110 | None:intergenic |
| AGTAGACGTCTTCCATCTCA+GGG | 0.629051 | 5:-94824116 | None:intergenic |
| TATAAAGGTTCGATGAGACT+CGG | 0.638209 | 5:+94824315 | MsG0580029292.01.T01:CDS |
| TCATGCACCTTGATGAACAC+AGG | 0.642184 | 5:-94824087 | None:intergenic |
| CCTACGAGAGACTCAGTCAC+CGG | 0.681169 | 5:-94824189 | None:intergenic |
| CCGGTGACTGAGTCTCTCGT+AGG | 0.681924 | 5:+94824189 | MsG0580029292.01.T01:CDS |
| TGTGAAAGTGATCAATGTCA+AGG | 0.686625 | 5:+94824143 | MsG0580029292.01.T01:CDS |
| GAGACTCGGTGTTCGCAAAG+CGG | 0.689421 | 5:+94824329 | MsG0580029292.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATCAATCGAATCAAAGA+GGG | + | Chr5:94824255-94824274 | MsG0580029292.01.T01:CDS | 25.0% |
| ! | TAAAATCAATCGAATCAAAG+AGG | + | Chr5:94824254-94824273 | MsG0580029292.01.T01:CDS | 25.0% |
| !!! | TGATTCGATTGATTTTATCA+CGG | - | Chr5:94824251-94824270 | None:intergenic | 25.0% |
| ACAATCTTCTTTGACTATGA+AGG | - | Chr5:94824385-94824404 | None:intergenic | 30.0% | |
| GCAAGGATACTTATGTATAA+AGG | + | Chr5:94824300-94824319 | MsG0580029292.01.T01:CDS | 30.0% | |
| TAGTCAAAGAAGATTGTAAC+TGG | + | Chr5:94824388-94824407 | MsG0580029292.01.T01:CDS | 30.0% | |
| ATCTTCATATTCAGAGCGAT+CGG | + | Chr5:94824225-94824244 | MsG0580029292.01.T01:CDS | 35.0% | |
| TATAAAGGTTCGATGAGACT+CGG | + | Chr5:94824315-94824334 | MsG0580029292.01.T01:CDS | 35.0% | |
| TGTGAAAGTGATCAATGTCA+AGG | + | Chr5:94824143-94824162 | MsG0580029292.01.T01:CDS | 35.0% | |
| ATCAACCATTATAGTGCGCA+AGG | + | Chr5:94824278-94824297 | MsG0580029292.01.T01:CDS | 40.0% | |
| GTCAAGGATGTGTCTCATAA+GGG | + | Chr5:94824159-94824178 | MsG0580029292.01.T01:CDS | 40.0% | |
| GTCTCATAAGGGTAAGATAC+CGG | + | Chr5:94824170-94824189 | MsG0580029292.01.T01:CDS | 40.0% | |
| TCTAACACCTGTGTTCATCA+AGG | + | Chr5:94824080-94824099 | MsG0580029292.01.T01:CDS | 40.0% | |
| TGTCAAGGATGTGTCTCATA+AGG | + | Chr5:94824158-94824177 | MsG0580029292.01.T01:CDS | 40.0% | |
| AAGTAGACGTCTTCCATCTC+AGG | - | Chr5:94824120-94824139 | None:intergenic | 45.0% | |
| AGTAGACGTCTTCCATCTCA+GGG | - | Chr5:94824119-94824138 | None:intergenic | 45.0% | |
| TCATGCACCTTGATGAACAC+AGG | - | Chr5:94824090-94824109 | None:intergenic | 45.0% | |
| TCTTTGACTATGAAGGCAGC+AGG | - | Chr5:94824378-94824397 | None:intergenic | 45.0% | |
| CCATTATAGTGCGCAAGGCA+AGG | + | Chr5:94824283-94824302 | MsG0580029292.01.T01:CDS | 50.0% | |
| CCTTGCCTTGCGCACTATAA+TGG | - | Chr5:94824286-94824305 | None:intergenic | 50.0% | |
| CCTACGAGAGACTCAGTCAC+CGG | - | Chr5:94824192-94824211 | None:intergenic | 55.0% | |
| TCTCTCGTAGGCGACGAAAC+TGG | + | Chr5:94824201-94824220 | MsG0580029292.01.T01:CDS | 55.0% | |
| !! | GAGACTCGGTGTTCGCAAAG+CGG | + | Chr5:94824329-94824348 | MsG0580029292.01.T01:CDS | 55.0% |
| CCGGTGACTGAGTCTCTCGT+AGG | + | Chr5:94824189-94824208 | MsG0580029292.01.T01:CDS | 60.0% | |
| CGTCTTCCATCTCAGGGCGT+AGG | - | Chr5:94824113-94824132 | None:intergenic | 60.0% | |
| GCATGACCTACGCCCTGAGA+TGG | + | Chr5:94824104-94824123 | MsG0580029292.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 94824075 | 94824449 | 94824075 | ID=MsG0580029292.01;Name=MsG0580029292.01 |
| Chr5 | mRNA | 94824075 | 94824449 | 94824075 | ID=MsG0580029292.01.T01;Parent=MsG0580029292.01;Name=MsG0580029292.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|124 |
| Chr5 | exon | 94824075 | 94824449 | 94824075 | ID=MsG0580029292.01.T01:exon:18268;Parent=MsG0580029292.01.T01 |
| Chr5 | CDS | 94824075 | 94824449 | 94824075 | ID=MsG0580029292.01.T01:cds;Parent=MsG0580029292.01.T01 |
| Chr5 | mRNA | 94824123 | 94824449 | 94824123 | ID=MsG0580029292.01.T02;Parent=MsG0580029292.01;Name=MsG0580029292.01.T02;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|0|1|0|108 |
| Chr5 | exon | 94824123 | 94824449 | 94824123 | ID=MsG0580029292.01.T02:exon:18269;Parent=MsG0580029292.01.T02 |
| Chr5 | CDS | 94824123 | 94824449 | 94824123 | ID=MsG0580029292.01.T02:cds;Parent=MsG0580029292.01.T02 |
| Gene Sequence |
| Protein sequence |
