AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030294.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030294.01.T01 MTR_6g004200 98.239 568 10 0 1 568 1 568 0.0 1147
MsG0680030294.01.T01 MTR_3g099190 26.042 576 365 18 6 553 80 622 2.42e-40 155
MsG0680030294.01.T01 MTR_2g027220 25.376 532 352 14 42 553 100 606 2.15e-39 152
MsG0680030294.01.T01 MTR_2g013120 25.000 492 327 12 6 480 84 550 2.13e-36 144
MsG0680030294.01.T01 MTR_2g015560 22.441 508 312 21 11 482 27 488 2.26e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030294.01.T01 AT5G17380 78.799 566 114 3 4 568 12 572 0.0 917
MsG0680030294.01.T01 AT3G48560 26.069 491 323 11 6 480 99 565 2.27e-39 153

Find 225 sgRNAs with CRISPR-Local

Find 321 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCATTACCTCCTTTAA+AGG 0.145440 6:+220699 MsG0680030294.01.T01:five_prime_UTR
TTCTCTAGTTCAGAAATTTA+TGG 0.158815 6:+224362 MsG0680030294.01.T01:three_prime_UTR
AGAGATGCTATTTCGGAATT+TGG 0.168057 6:+221999 MsG0680030294.01.T01:CDS
TTGAATTGGCTGCTTCATTT+TGG 0.184186 6:+221696 MsG0680030294.01.T01:CDS
TCGACCTGGTGGTGTTTATT+TGG 0.219372 6:+221308 MsG0680030294.01.T01:CDS
AATGAGAGGTTGGCCTTTAA+AGG 0.220329 6:-220712 None:intergenic
AGGACCTGTGCGACGCAATT+TGG 0.254300 6:-221267 None:intergenic
TAGGTTGGATGCTGGTACTT+GGG 0.254929 6:+222109 MsG0680030294.01.T01:CDS
GTCAATTTCTCCCATTGAAT+TGG 0.270290 6:-220848 None:intergenic
GTTCTAACGGAGAGACTCTT+TGG 0.274270 6:+220595 MsG0680030294.01.T01:five_prime_UTR
GAGAGGCCGTTGATTGTGTT+TGG 0.282927 6:+221489 MsG0680030294.01.T01:CDS
GTTGCTGTTGAAGGGGATTC+TGG 0.306147 6:+222197 MsG0680030294.01.T01:CDS
CACCGCGACCGACATCGTTT+TGG 0.308872 6:-221164 None:intergenic
TGGTGATGCTAAAGAAGTTT+TGG 0.310510 6:+221812 MsG0680030294.01.T01:CDS
GTTGAAGGGGATTCTGGATA+TGG 0.312883 6:+222203 MsG0680030294.01.T01:CDS
TGCGACGCAATTTGGAATTT+CGG 0.318967 6:-221259 None:intergenic
TTCAACAATGGTGGTGTATA+TGG 0.321717 6:+223788 MsG0680030294.01.T01:CDS
CCCTTTCTTCCGACTCCAAT+GGG 0.325610 6:+221576 MsG0680030294.01.T01:CDS
TCCGGTCCCGGTTGCGTTCA+CGG 0.331596 6:+221075 MsG0680030294.01.T01:CDS
TGAAGATGAATTGAAGAAAT+TGG 0.345002 6:+221536 MsG0680030294.01.T01:CDS
CGCCTTGTCGTTGCTGTTGA+AGG 0.349466 6:+222188 MsG0680030294.01.T01:CDS
TTCTCCCATTGAATTGGAAA+TGG 0.349946 6:-220842 None:intergenic
TTCACATCCTTAGACCATTT+CGG 0.350348 6:-221726 None:intergenic
GGCTAGACCGTGAACGCAAC+CGG 0.353254 6:-221082 None:intergenic
GTGGGAAGGGTTATCTTGTT+GGG 0.362279 6:+223918 MsG0680030294.01.T01:CDS
CTAGGTTGGATGCTGGTACT+TGG 0.365720 6:+222108 MsG0680030294.01.T01:CDS
AATTTAGGATGAAGAGTTGT+AGG 0.367668 6:+224265 MsG0680030294.01.T01:three_prime_UTR
AAAATTCAAACCAAACAGTT+TGG 0.370575 6:+224390 MsG0680030294.01.T01:three_prime_UTR
GGTGGGAAGGGTTATCTTGT+TGG 0.370731 6:+223917 MsG0680030294.01.T01:CDS
GATTATCAGAGATGCTATTT+CGG 0.371238 6:+221992 MsG0680030294.01.T01:CDS
GAAAATTACTAAGTTATGTA+TGG 0.372192 6:-220809 None:intergenic
ACTTGGCATTGGCAAGTGAT+AGG 0.378771 6:-224075 None:intergenic
AGAGGCCGTTGATTGTGTTT+GGG 0.386929 6:+221490 MsG0680030294.01.T01:CDS
ATAATCCATTTCCAATTCAA+TGG 0.394628 6:+220837 MsG0680030294.01.T01:exon
TAAAACTGAACCAAACTGTT+TGG 0.400106 6:-224400 None:intergenic
GCGGCTAGGTCACTTGCTAT+TGG 0.400777 6:+221639 MsG0680030294.01.T01:CDS
CACACTATGATGGAAGCTTT+TGG 0.408547 6:+223896 MsG0680030294.01.T01:CDS
GGTTAAAAGCGATTCAGCTT+CGG 0.413715 6:-221370 None:intergenic
AAGAAATTGGTGGAAAGGAC+AGG 0.415680 6:+221549 MsG0680030294.01.T01:CDS
TTCCTCACCGTCTCCGGTCC+CGG 0.420654 6:+221063 MsG0680030294.01.T01:CDS
AGAGCTGACTTTAGTTCATC+AGG 0.420866 6:-223944 None:intergenic
GCTTGGGGAGCACTCATCCA+TGG 0.422593 6:+221871 MsG0680030294.01.T01:CDS
ATAGTGTGGTACCCTGCATT+TGG 0.425828 6:-223881 None:intergenic
AGCTGTTTCGCTTCTGAGAA+AGG 0.431667 6:+221464 MsG0680030294.01.T01:CDS
GGAAGAGCCGAAATGGTCTA+AGG 0.431832 6:+221719 MsG0680030294.01.T01:CDS
GTAAGCGGAGGCAGCGTAAC+CGG 0.431967 6:-221013 None:intergenic
GGCATCTTCCTCACCGTCTC+CGG 0.433782 6:+221057 MsG0680030294.01.T01:CDS
CGTTGGCTGCCTCCGCGGCT+AGG 0.435971 6:+221625 MsG0680030294.01.T01:CDS
TAATCCATTTCCAATTCAAT+GGG 0.438198 6:+220838 MsG0680030294.01.T01:exon
GAACCAGTGTCATGTGTAAC+TGG 0.440341 6:+220553 MsG0680030294.01.T01:five_prime_UTR
AATGGGGAAGGGATTGTTGC+CGG 0.440749 6:+221593 MsG0680030294.01.T01:CDS
ACATCCTTTGTTCCAAATGC+AGG 0.441014 6:+223869 MsG0680030294.01.T01:CDS
GCAGCCAACGGATGATCATC+CGG 0.446211 6:-221612 None:intergenic
TGGCCTACTGTCATGATCTC+TGG 0.446356 6:+221132 MsG0680030294.01.T01:CDS
ACCGTGAACGCAACCGGGAC+CGG 0.449721 6:-221076 None:intergenic
GGATGTGAAGTTTGTGTTGG+TGG 0.452229 6:+221740 MsG0680030294.01.T01:CDS
TCAGAAACCACCACAGGAGC+TGG 0.456175 6:-222029 None:intergenic
TCCCTTTCTTCCGACTCCAA+TGG 0.456521 6:+221575 MsG0680030294.01.T01:CDS
GTTTCTCTTCCGCGCTTCTA+CGG 0.457373 6:-223817 None:intergenic
GCGTGTGGCGAGTGATGTTA+CGG 0.458645 6:-220941 None:intergenic
CTTGGGGAGCACTCATCCAT+GGG 0.462647 6:+221872 MsG0680030294.01.T01:CDS
GCCTTGTCGTTGCTGTTGAA+GGG 0.464496 6:+222189 MsG0680030294.01.T01:CDS
TGTTATTATTATATATATGT+TGG 0.466702 6:-220739 None:intergenic
CCCATTGGAGTCGGAAGAAA+GGG 0.471741 6:-221576 None:intergenic
AGGTTGGATGCTGGTACTTG+GGG 0.472904 6:+222110 MsG0680030294.01.T01:CDS
CCCCATTGGAGTCGGAAGAA+AGG 0.474099 6:-221577 None:intergenic
TGATTGGTGCGAGGTTGAAT+TGG 0.474694 6:+221682 MsG0680030294.01.T01:CDS
GGAACAAAGGATGTCGGAGC+GGG 0.475357 6:-223860 None:intergenic
GCTAAATCCTTCTCTCAATT+CGG 0.476034 6:+220886 MsG0680030294.01.T01:CDS
CATGACACATCCTCATTAGT+TGG 0.479447 6:+220511 None:intergenic
TTATTTATCAATTAATTGAA+AGG 0.480473 6:-224209 None:intergenic
ATGGTAACATTTATGACGGC+TGG 0.480846 6:-223992 None:intergenic
TGCTAGCATTCACTTGGCAT+TGG 0.480966 6:-224086 None:intergenic
TTCGGCGTCCAACACATGTT+CGG 0.481163 6:+220904 MsG0680030294.01.T01:CDS
CCGTTGATTGTGTTTGGGAA+AGG 0.481200 6:+221495 MsG0680030294.01.T01:CDS
GGTTACGCTGCCTCCGCTTA+CGG 0.482147 6:+221015 MsG0680030294.01.T01:CDS
TGTGAAGATGGAGGCTCAAT+TGG 0.483919 6:+221929 MsG0680030294.01.T01:CDS
GCTCCTGTGGTGGTTTCTGA+AGG 0.488005 6:+222032 MsG0680030294.01.T01:CDS
TTCTTCAACTTCATTCCTTG+TGG 0.488787 6:+224118 MsG0680030294.01.T01:three_prime_UTR
GGTGAGGAAGATGCCGGGAC+GGG 0.489548 6:-221049 None:intergenic
CTTCCGACTCCAATGGGGAA+GGG 0.490349 6:+221582 MsG0680030294.01.T01:CDS
CGTGTGGCGAGTGATGTTAC+GGG 0.494034 6:-220940 None:intergenic
GACAGTAGGCCATGTGTTAG+TGG 0.494295 6:-221121 None:intergenic
CTCGAAATAGCTTCCACCCA+TGG 0.495380 6:-221888 None:intergenic
TTCTAACGGAGAGACTCTTT+GGG 0.495850 6:+220596 MsG0680030294.01.T01:five_prime_UTR
CCCTATGCCGGTTCTGAGAG+TGG 0.502759 6:+224019 MsG0680030294.01.T01:CDS
AGTGGTGCCGTTGGAGAGAC+CGG 0.505297 6:-221103 None:intergenic
ATTATTATATATATGTTGGA+TGG 0.506836 6:-220735 None:intergenic
TTGTAGGTGGAATGATAAAC+CGG 0.510749 6:+224281 MsG0680030294.01.T01:three_prime_UTR
AACAACAGATACAAAATTGA+GGG 0.512141 6:-220639 None:intergenic
GAGGAAGATGCCGGGACGGG+AGG 0.516089 6:-221046 None:intergenic
GCATCTCTGATAATCCTCAT+TGG 0.517324 6:-221984 None:intergenic
ACAGCAACGACAAGGCGATC+AGG 0.517340 6:-222182 None:intergenic
TCTCAGAATCTTTCTCTGCT+AGG 0.519344 6:+223966 MsG0680030294.01.T01:CDS
GAAGGACAATGGTGTGAAGA+TGG 0.519995 6:+221917 MsG0680030294.01.T01:CDS
AACGCAACCGGGACCGGAGA+CGG 0.521595 6:-221070 None:intergenic
AATTCAATGGGAGAAATTGA+CGG 0.523138 6:+220850 MsG0680030294.01.T01:exon
GGCAACAATCCCTTCCCCAT+TGG 0.524820 6:-221591 None:intergenic
AACCAAAACGATGTCGGTCG+CGG 0.525927 6:+221162 MsG0680030294.01.T01:CDS
AATTGAAGAAATTGGTGGAA+AGG 0.526177 6:+221544 MsG0680030294.01.T01:CDS
CCAGCATCCAACCTAGTCCT+TGG 0.526297 6:-222101 None:intergenic
CCTTGTCGTTGCTGTTGAAG+GGG 0.527216 6:+222190 MsG0680030294.01.T01:CDS
CATCCTCCCACTCTCAGAAC+CGG 0.529633 6:-224026 None:intergenic
GTTACCATTGATCCCTATGC+CGG 0.530592 6:+224007 MsG0680030294.01.T01:CDS
CAACACATGTTCGGCGTCGT+CGG 0.531298 6:+220913 MsG0680030294.01.T01:CDS
GCCACACGCGCCGTCTCTCT+CGG 0.531512 6:+220955 MsG0680030294.01.T01:CDS
TATTTATCAATTAATTGAAA+GGG 0.532330 6:-224208 None:intergenic
CAACAACAGATACAAAATTG+AGG 0.533417 6:-220640 None:intergenic
TGGATATGGATTTAGTGCTA+TGG 0.537673 6:+222217 MsG0680030294.01.T01:CDS
GCCGAGAGAGACGGCGCGTG+TGG 0.538337 6:-220956 None:intergenic
TGGTCTTGGTTATTGTATTG+CGG 0.538505 6:+222145 MsG0680030294.01.T01:CDS
TGTGACGTGGCGATTGTGAT+TGG 0.538530 6:+221666 MsG0680030294.01.T01:CDS
TGTGTTGGTGGATGTGAGCA+AGG 0.538770 6:+221752 MsG0680030294.01.T01:CDS
TCGATCTGTGTTGGTTCAGA+AGG 0.541457 6:+222076 MsG0680030294.01.T01:CDS
GGTTGCGTTCACGGTCTAGC+CGG 0.543093 6:+221084 MsG0680030294.01.T01:CDS
AAGGAATGAAGTTGAAGAAG+TGG 0.544457 6:-224114 None:intergenic
CCCACTCTCAGAACCGGCAT+AGG 0.547084 6:-224020 None:intergenic
AATTCCAAATTGCGTCGCAC+AGG 0.547940 6:+221263 MsG0680030294.01.T01:CDS
CGGTGAGGAAGATGCCGGGA+CGG 0.553454 6:-221050 None:intergenic
CCACTCTCAGAACCGGCATA+GGG 0.554364 6:-224019 None:intergenic
TAAGGATGTGAAGTTTGTGT+TGG 0.554761 6:+221737 MsG0680030294.01.T01:CDS
AAAATTACTAAGTTATGTAT+GGG 0.554804 6:-220808 None:intergenic
GGCTACCTCACCTCCCGTCC+CGG 0.554854 6:+221036 MsG0680030294.01.T01:CDS
TGCAGGGTACCACACTATGA+TGG 0.554969 6:+223886 MsG0680030294.01.T01:CDS
AGATGCCGGGACGGGAGGTG+AGG 0.556090 6:-221041 None:intergenic
GAAGGAGCCAAGGACTAGGT+TGG 0.558905 6:+222094 MsG0680030294.01.T01:CDS
TTCAGAAGGAGCCAAGGACT+AGG 0.561809 6:+222090 MsG0680030294.01.T01:CDS
AGAACCGGCATAGGGATCAA+TGG 0.563367 6:-224011 None:intergenic
TCTTCCGACTCCAATGGGGA+AGG 0.563603 6:+221581 MsG0680030294.01.T01:CDS
AGGTGGAATGATAAACCGGC+TGG 0.565803 6:+224285 MsG0680030294.01.T01:three_prime_UTR
GGATGTTGGTCGATCTGTGT+TGG 0.566745 6:+222067 MsG0680030294.01.T01:CDS
ATTTAGTGCTATGGAAGTTG+AGG 0.568805 6:+222226 MsG0680030294.01.T01:CDS
CCTATGCCGGTTCTGAGAGT+GGG 0.571561 6:+224020 MsG0680030294.01.T01:CDS
CTGAGTCAGCAAGGTTCTAA+CGG 0.572977 6:+220582 MsG0680030294.01.T01:five_prime_UTR
TGTTGCATTACAACTCCAGC+CGG 0.575250 6:-224300 None:intergenic
GAAACGGATGCCGAGAGAGA+CGG 0.575910 6:-220965 None:intergenic
ATCACATTCACCAACTAATG+AGG 0.576403 6:-220521 None:intergenic
AGATGAATTGAAGAAATTGG+TGG 0.576890 6:+221539 MsG0680030294.01.T01:CDS
GGTTGGATGCTGGTACTTGG+GGG 0.577192 6:+222111 MsG0680030294.01.T01:CDS
CCAAGGACTAGGTTGGATGC+TGG 0.578727 6:+222101 MsG0680030294.01.T01:CDS
TGAGTGCTCCCCAAGCAAAA+CGG 0.579880 6:-221864 None:intergenic
GATGCTATTTCGGAATTTGG+AGG 0.580533 6:+222002 MsG0680030294.01.T01:CDS
GAGACGGTGAGGAAGATGCC+GGG 0.580765 6:-221054 None:intergenic
TGGAGGAAGTCCAGCTCCTG+TGG 0.581328 6:+222019 MsG0680030294.01.T01:CDS
ATCAATGGTAACATTTATGA+CGG 0.582311 6:-223996 None:intergenic
GGTGAGGTAGCCGTAAGCGG+AGG 0.583833 6:-221025 None:intergenic
AGAAATTGGTGGAAAGGACA+GGG 0.584953 6:+221550 MsG0680030294.01.T01:CDS
CGACGACGCCGAACATGTGT+TGG 0.585822 6:-220912 None:intergenic
GAAACATGGACCTCACAAGA+AGG 0.587068 6:+223835 MsG0680030294.01.T01:CDS
CACAGATCGACCAACATCCA+TGG 0.588090 6:-222063 None:intergenic
AGGAAGTCCAGCTCCTGTGG+TGG 0.588129 6:+222022 MsG0680030294.01.T01:CDS
TTGAAAACAATCACCACCAC+AGG 0.588388 6:-223770 None:intergenic
CGTTGTGGAAAGCAATGAAA+CGG 0.588416 6:-220981 None:intergenic
GCTAGACCGTGAACGCAACC+GGG 0.589274 6:-221081 None:intergenic
CATCCTTTGTTCCAAATGCA+GGG 0.589443 6:+223870 MsG0680030294.01.T01:CDS
AATTCCGTTGCGAATCGACC+TGG 0.589507 6:+221294 MsG0680030294.01.T01:CDS
TTCTGATGCAATACATCAGT+AGG 0.589507 6:-221336 None:intergenic
AACCGGCGGATTGTTCGTTG+TGG 0.592058 6:-220996 None:intergenic
CTAGCCGCGGAGGCAGCCAA+CGG 0.593647 6:-221624 None:intergenic
CCTTTCCCAAACACAATCAA+CGG 0.595147 6:-221495 None:intergenic
TTCTGAAGGTGCTAATACCA+TGG 0.595296 6:+222046 MsG0680030294.01.T01:CDS
GAAGGCTGCTAGCATTCACT+TGG 0.597581 6:-224092 None:intergenic
AATCCCTTCCCCATTGGAGT+CGG 0.598920 6:-221585 None:intergenic
GGAGACGGTGAGGAAGATGC+CGG 0.599476 6:-221055 None:intergenic
CCATGTGTTAGTGGTGCCGT+TGG 0.601490 6:-221112 None:intergenic
AGCTATTTCGAGTAAAGTGA+AGG 0.602234 6:+221899 MsG0680030294.01.T01:CDS
CCCCTTCAACAGCAACGACA+AGG 0.603202 6:-222190 None:intergenic
GGAGGTGAGGTAGCCGTAAG+CGG 0.606197 6:-221028 None:intergenic
GAGAGGTTGGCCTTTAAAGG+AGG 0.607115 6:-220709 None:intergenic
TTAGGATGAAGAGTTGTAGG+TGG 0.612187 6:+224268 MsG0680030294.01.T01:three_prime_UTR
TTGGACGCCGAATTGAGAGA+AGG 0.613367 6:-220893 None:intergenic
TTCCACAACGAACAATCCGC+CGG 0.614198 6:+220994 MsG0680030294.01.T01:CDS
TCTTGTAACCAAAACGATGT+CGG 0.617651 6:+221156 MsG0680030294.01.T01:CDS
TGGTGGAGGCTGAGTCAGCA+AGG 0.617989 6:+220573 MsG0680030294.01.T01:five_prime_UTR
TGGTGAATGTGATGTGAGGG+AGG 0.619242 6:+220531 MsG0680030294.01.T01:five_prime_UTR
TGTTGGATGGAATGAGAGGT+TGG 0.619941 6:-220722 None:intergenic
GATGGAGGCTCAATTGGCAA+AGG 0.622460 6:+221935 MsG0680030294.01.T01:CDS
CAATCACCACCACAGGCAGT+TGG 0.622614 6:-223763 None:intergenic
AACCGGGACCGGAGACGGTG+AGG 0.622684 6:-221065 None:intergenic
TGGAACAAAGGATGTCGGAG+CGG 0.622773 6:-223861 None:intergenic
TGTTGGTTCAGAAGGAGCCA+AGG 0.626040 6:+222084 MsG0680030294.01.T01:CDS
AATGAAGTTGAAGAAGTGGA+AGG 0.626692 6:-224110 None:intergenic
AGCAAGTGACCTAGCCGCGG+AGG 0.628473 6:-221634 None:intergenic
GTTGCCGGATGATCATCCGT+TGG 0.630066 6:+221608 MsG0680030294.01.T01:CDS
GCATTTGGAACAAAGGATGT+CGG 0.630283 6:-223866 None:intergenic
ATGCCGGTTCTGAGAGTGGG+AGG 0.630890 6:+224023 MsG0680030294.01.T01:CDS
GTGTCATGTGTAACTGGTGG+AGG 0.631341 6:+220559 MsG0680030294.01.T01:five_prime_UTR
TCGAGTAAAGTGAAGGACAA+TGG 0.631645 6:+221906 MsG0680030294.01.T01:CDS
CGCTTCTGAGAAAGGCGGAG+AGG 0.633700 6:+221472 MsG0680030294.01.T01:CDS
GGAGCGGGGTCCTTCTTGTG+AGG 0.634878 6:-223845 None:intergenic
ACCACCAGGTCGATTCGCAA+CGG 0.637403 6:-221298 None:intergenic
TCCGTTGCGAATCGACCTGG+TGG 0.638885 6:+221297 MsG0680030294.01.T01:CDS
AGCGGAGGCAGCGTAACCGG+CGG 0.642708 6:-221010 None:intergenic
GGTCTAGCCGGTCTCTCCAA+CGG 0.643085 6:+221096 MsG0680030294.01.T01:CDS
AGATCCAAATAAACACCACC+AGG 0.649375 6:-221312 None:intergenic
AGTTGGTGAATGTGATGTGA+GGG 0.650518 6:+220528 MsG0680030294.01.T01:five_prime_UTR
GAACCAGAGATCATGACAGT+AGG 0.653809 6:-221135 None:intergenic
GATTCGCAACGGAATTAGCA+AGG 0.654780 6:-221287 None:intergenic
CCAACGGCACCACTAACACA+TGG 0.655427 6:+221112 MsG0680030294.01.T01:CDS
AACAGATACAAAATTGAGGG+AGG 0.656662 6:-220636 None:intergenic
AGAAGCGCGGAAGAGAAACA+TGG 0.658183 6:+223821 MsG0680030294.01.T01:CDS
GGTGCTAATACCATGGATGT+TGG 0.661368 6:+222053 MsG0680030294.01.T01:CDS
TAGTTGGTGAATGTGATGTG+AGG 0.661800 6:+220527 MsG0680030294.01.T01:five_prime_UTR
CCACCAGTTACACATGACAC+TGG 0.663449 6:-220556 None:intergenic
GCGATCAGGATAAGCGACAG+TGG 0.665456 6:-222168 None:intergenic
GAACAAAGGATGTCGGAGCG+GGG 0.667800 6:-223859 None:intergenic
GCACCTTCAGAAACCACCAC+AGG 0.667938 6:-222035 None:intergenic
TCGGTACCAACTGCCTGTGG+TGG 0.670360 6:+223757 MsG0680030294.01.T01:CDS
CAAAAGCTTCCATCATAGTG+TGG 0.672720 6:-223895 None:intergenic
TCATCCGTTGGCTGCCTCCG+CGG 0.676715 6:+221620 MsG0680030294.01.T01:CDS
TGGTGGTGACCGTAGAAGCG+CGG 0.676748 6:+223808 MsG0680030294.01.T01:CDS
GGGGAGCACTCATCCATGGG+TGG 0.677623 6:+221875 MsG0680030294.01.T01:CDS
TGTTTCGCTTCTGAGAAAGG+CGG 0.679005 6:+221467 MsG0680030294.01.T01:CDS
GTACCCTGCATTTGGAACAA+AGG 0.679208 6:-223873 None:intergenic
TGGCGATTGTGATTGGTGCG+AGG 0.681842 6:+221673 MsG0680030294.01.T01:CDS
CCAGTGTCATGTGTAACTGG+TGG 0.684852 6:+220556 MsG0680030294.01.T01:five_prime_UTR
CCTTTCTTCCGACTCCAATG+GGG 0.685812 6:+221577 MsG0680030294.01.T01:CDS
GGTTCGGTACCAACTGCCTG+TGG 0.691668 6:+223754 MsG0680030294.01.T01:CDS
TATATGTTGGATGGAATGAG+AGG 0.694995 6:-220726 None:intergenic
AATAGCAAGTGACCTAGCCG+CGG 0.695771 6:-221637 None:intergenic
GGACAATGGTGTGAAGATGG+AGG 0.707741 6:+221920 MsG0680030294.01.T01:CDS
AACAATGGTGGTGTATATGG+TGG 0.712302 6:+223791 MsG0680030294.01.T01:CDS
TGCTATTGGAAAATGTGACG+TGG 0.744033 6:+221653 MsG0680030294.01.T01:CDS
GTGTGGCGAGTGATGTTACG+GGG 0.745882 6:-220939 None:intergenic
GAGAGAAGGATTTAGCAGCG+AGG 0.776384 6:-220879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAAAAAA+AGG + Chr6:222429-222448 MsG0680030294.01.T01:intron 0.0%
!! AAAAAAAAAAAAAAAAAAAA+GGG + Chr6:222429-222448 MsG0680030294.01.T01:intron 0.0%
!! TATTTATCAATTAATTGAAA+GGG - Chr6:224211-224230 None:intergenic 10.0%
!! TGTTATTATTATATATATGT+TGG - Chr6:220742-220761 None:intergenic 10.0%
!! TTATTTATCAATTAATTGAA+AGG - Chr6:224212-224231 None:intergenic 10.0%
!!! AAAAAAAAAAAAAGGTTTTT+TGG + Chr6:222535-222554 MsG0680030294.01.T01:intron 10.0%
!!! CATATTAGTTTATAAATTTT+TGG + Chr6:223609-223628 MsG0680030294.01.T01:intron 10.0%
!!! TTTTTTTTTTTTCAATTTCA+AGG - Chr6:222421-222440 None:intergenic 10.0%
!! AAAATTACTAAGTTATGTAT+GGG - Chr6:220811-220830 None:intergenic 15.0%
!! ATTATTATATATATGTTGGA+TGG - Chr6:220738-220757 None:intergenic 15.0%
!! TATAAAATTTGAAAAATCTG+CGG - Chr6:223082-223101 None:intergenic 15.0%
!! TATAAACTAATATGAATGAA+TGG - Chr6:223603-223622 None:intergenic 15.0%
!!! AAAAAAAAAAAAAGGGTATT+TGG + Chr6:222440-222459 MsG0680030294.01.T01:intron 15.0%
!!! TACTTTTAGATAATCAATTT+AGG + Chr6:224250-224269 MsG0680030294.01.T01:three_prime_UTR 15.0%
!! AATGTGATCTTATTCATATA+TGG + Chr6:223647-223666 MsG0680030294.01.T01:intron 20.0%
!! ATAAAATTTGAAAAATCTGC+GGG - Chr6:223081-223100 None:intergenic 20.0%
!! ATTCTTGTATTCATAAAATG+AGG - Chr6:223695-223714 None:intergenic 20.0%
!! GAAAATTACTAAGTTATGTA+TGG - Chr6:220812-220831 None:intergenic 20.0%
!!! ACATCAGTAATTGATTTAAA+TGG - Chr6:223158-223177 None:intergenic 20.0%
!!! CTTAACAAGTTTGTTATTTT+GGG + Chr6:222670-222689 MsG0680030294.01.T01:intron 20.0%
!!! CTTGTTAAGAAATTTTGATA+AGG - Chr6:222659-222678 None:intergenic 20.0%
!!! TCTTAACAAGTTTGTTATTT+TGG + Chr6:222669-222688 MsG0680030294.01.T01:intron 20.0%
!!! TTTTTTTTTCTTTCAGACAT+TGG + Chr6:223733-223752 MsG0680030294.01.T01:intron 20.0%
! AAAATTCAAACCAAACAGTT+TGG + Chr6:224390-224409 MsG0680030294.01.T01:three_prime_UTR 25.0%
! AAATTGAACAAAATGCTGTT+TGG + Chr6:223271-223290 MsG0680030294.01.T01:intron 25.0%
! AACAACAGATACAAAATTGA+GGG - Chr6:220642-220661 None:intergenic 25.0%
! AAGTAAAGACCTTAATAACA+AGG - Chr6:223441-223460 None:intergenic 25.0%
! ACATACACATATTAGATTTG+TGG + Chr6:222627-222646 MsG0680030294.01.T01:intron 25.0%
! ATAATCCATTTCCAATTCAA+TGG + Chr6:220837-220856 MsG0680030294.01.T01:exon 25.0%
! ATATAACTTCTATAGTCTCA+GGG - Chr6:222708-222727 None:intergenic 25.0%
! ATCAATGGTAACATTTATGA+CGG - Chr6:223999-224018 None:intergenic 25.0%
! ATGTTCTTATGCTTGTAAAA+TGG + Chr6:223533-223552 MsG0680030294.01.T01:intron 25.0%
! ATTGGCGTCAAAAAATTAAA+CGG - Chr6:221969-221988 None:intergenic 25.0%
! GAAATTGAAAAAAGGTTGTT+TGG + Chr6:222910-222929 MsG0680030294.01.T01:intron 25.0%
! TAATCCATTTCCAATTCAAT+GGG + Chr6:220838-220857 MsG0680030294.01.T01:exon 25.0%
! TATATAACTTCTATAGTCTC+AGG - Chr6:222709-222728 None:intergenic 25.0%
! TATCAAAGATCTTTACTATC+AGG - Chr6:222846-222865 None:intergenic 25.0%
! TGAAGATGAATTGAAGAAAT+TGG + Chr6:221536-221555 MsG0680030294.01.T01:CDS 25.0%
! TTGTCCTTGAAATTGAAAAA+AGG + Chr6:222414-222433 MsG0680030294.01.T01:intron 25.0%
!! TTCTCTAGTTCAGAAATTTA+TGG + Chr6:224362-224381 MsG0680030294.01.T01:three_prime_UTR 25.0%
!!! ACAACCTTTTTTCAATTTCA+AGG - Chr6:222909-222928 None:intergenic 25.0%
!!! AGTTTTGGAGATTTTGAATA+AGG + Chr6:221827-221846 MsG0680030294.01.T01:CDS 25.0%
AAACAAAAAACTGAACCACA+AGG - Chr6:224136-224155 None:intergenic 30.0%
AATTCAATGGGAGAAATTGA+CGG + Chr6:220850-220869 MsG0680030294.01.T01:exon 30.0%
AATTGAAGAAATTGGTGGAA+AGG + Chr6:221544-221563 MsG0680030294.01.T01:CDS 30.0%
AGATGAATTGAAGAAATTGG+TGG + Chr6:221539-221558 MsG0680030294.01.T01:CDS 30.0%
ATAAAACTTGCATAGTCTCA+GGG - Chr6:223466-223485 None:intergenic 30.0%
ATCAATTACTGATGTTGTGA+AGG + Chr6:223163-223182 MsG0680030294.01.T01:intron 30.0%
CAAAATGTATTCTCAGTTTC+AGG - Chr6:223501-223520 None:intergenic 30.0%
CAACAACAGATACAAAATTG+AGG - Chr6:220643-220662 None:intergenic 30.0%
CCTGTTAAGAAGTTATCATA+AGG - Chr6:223398-223417 None:intergenic 30.0%
CCTTATGATAACTTCTTAAC+AGG + Chr6:223395-223414 MsG0680030294.01.T01:intron 30.0%
GATGATATAGACATAAGTAG+CGG + Chr6:222374-222393 MsG0680030294.01.T01:intron 30.0%
GCTACATCAGAGATTTAAAA+TGG - Chr6:222791-222810 None:intergenic 30.0%
TATAAAACTTGCATAGTCTC+AGG - Chr6:223467-223486 None:intergenic 30.0%
TCATTTGCAACAAAGAAGTT+GGG - Chr6:222595-222614 None:intergenic 30.0%
TGTAACATCCAAATCCAATA+GGG - Chr6:222330-222349 None:intergenic 30.0%
TTGTAACATCCAAATCCAAT+AGG - Chr6:222331-222350 None:intergenic 30.0%
! AATTTAGGATGAAGAGTTGT+AGG + Chr6:224265-224284 MsG0680030294.01.T01:three_prime_UTR 30.0%
! AGATACATATCTGAGATATG+TGG + Chr6:223366-223385 MsG0680030294.01.T01:intron 30.0%
! AGATCTTTGATAGTTAGTAG+CGG + Chr6:222854-222873 MsG0680030294.01.T01:intron 30.0%
! CAGCATTTTGTTCAATTTCA+TGG - Chr6:223269-223288 None:intergenic 30.0%
! GATTATCAGAGATGCTATTT+CGG + Chr6:221992-222011 MsG0680030294.01.T01:CDS 30.0%
! TTGATTGCGAATTTTGTGAA+AGG - Chr6:221225-221244 None:intergenic 30.0%
!! AGCTTAGAAAACCGTTTTTA+GGG + Chr6:221784-221803 MsG0680030294.01.T01:CDS 30.0%
!! TCAGTTTAGCCTTGTTATTA+AGG + Chr6:223429-223448 MsG0680030294.01.T01:intron 30.0%
!! TTTTCTTTCAGACATTGGTT+CGG + Chr6:223738-223757 MsG0680030294.01.T01:intron 30.0%
!!! TTAATTTTTTGACGCCAATG+AGG + Chr6:221970-221989 MsG0680030294.01.T01:CDS 30.0%
AACAGATACAAAATTGAGGG+AGG - Chr6:220639-220658 None:intergenic 35.0%
AAGGAATGAAGTTGAAGAAG+TGG - Chr6:224117-224136 None:intergenic 35.0%
AATGAAGTTGAAGAAGTGGA+AGG - Chr6:224113-224132 None:intergenic 35.0%
AATGTGTTTCTCTAGTCTAG+TGG + Chr6:222349-222368 MsG0680030294.01.T01:intron 35.0%
ACTTCTTTGTTGCAAATGAG+TGG + Chr6:222596-222615 MsG0680030294.01.T01:intron 35.0%
ATCACATTCACCAACTAATG+AGG - Chr6:220524-220543 None:intergenic 35.0%
ATTTAGTGCTATGGAAGTTG+AGG + Chr6:222226-222245 MsG0680030294.01.T01:CDS 35.0%
CTCATTTGCAACAAAGAAGT+TGG - Chr6:222596-222615 None:intergenic 35.0%
CTTCTCATTACCTCCTTTAA+AGG + Chr6:220699-220718 MsG0680030294.01.T01:five_prime_UTR 35.0%
CTTCTTTGTTGCAAATGAGT+GGG + Chr6:222597-222616 MsG0680030294.01.T01:intron 35.0%
GCTAAATCCTTCTCTCAATT+CGG + Chr6:220886-220905 MsG0680030294.01.T01:CDS 35.0%
GGCAAAGACCTAAAATAACA+AGG - Chr6:223053-223072 None:intergenic 35.0%
GTCAATTTCTCCCATTGAAT+TGG - Chr6:220851-220870 None:intergenic 35.0%
TAAGGATGTGAAGTTTGTGT+TGG + Chr6:221737-221756 MsG0680030294.01.T01:CDS 35.0%
TATATGTTGGATGGAATGAG+AGG - Chr6:220729-220748 None:intergenic 35.0%
TCACCAACTAACCCTAAAAA+CGG - Chr6:221798-221817 None:intergenic 35.0%
TCTGAACGAGTTATTTACTC+TGG + Chr6:223025-223044 MsG0680030294.01.T01:intron 35.0%
TCTTGTAACCAAAACGATGT+CGG + Chr6:221156-221175 MsG0680030294.01.T01:CDS 35.0%
TGGATATGGATTTAGTGCTA+TGG + Chr6:222217-222236 MsG0680030294.01.T01:CDS 35.0%
TTAAATCTCTGATGTAGCGA+GGG + Chr6:222793-222812 MsG0680030294.01.T01:intron 35.0%
TTCACATCCTTAGACCATTT+CGG - Chr6:221729-221748 None:intergenic 35.0%
TTCTCCCATTGAATTGGAAA+TGG - Chr6:220845-220864 None:intergenic 35.0%
TTCTGATGCAATACATCAGT+AGG - Chr6:221339-221358 None:intergenic 35.0%
TTCTTCAACTTCATTCCTTG+TGG + Chr6:224118-224137 MsG0680030294.01.T01:three_prime_UTR 35.0%
TTGAAAAATCTGCGGGAAAA+AGG - Chr6:223074-223093 None:intergenic 35.0%
TTGTAGGTGGAATGATAAAC+CGG + Chr6:224281-224300 MsG0680030294.01.T01:three_prime_UTR 35.0%
TTTAAATCTCTGATGTAGCG+AGG + Chr6:222792-222811 MsG0680030294.01.T01:intron 35.0%
! AAAGATGATCCGTTTTGCTT+GGG + Chr6:221855-221874 MsG0680030294.01.T01:CDS 35.0%
! AGCTATTTCGAGTAAAGTGA+AGG + Chr6:221899-221918 MsG0680030294.01.T01:CDS 35.0%
! CTAGTTTGCAACATAGAAGT+TGG - Chr6:223332-223351 None:intergenic 35.0%
! CTCGTTCAGAAGTTTTCATA+AGG - Chr6:223015-223034 None:intergenic 35.0%
! GTGATTGTTTTCAACAATGG+TGG + Chr6:223779-223798 MsG0680030294.01.T01:CDS 35.0%
! GTGGTGATTGTTTTCAACAA+TGG + Chr6:223776-223795 MsG0680030294.01.T01:CDS 35.0%
! TAAAGATGATCCGTTTTGCT+TGG + Chr6:221854-221873 MsG0680030294.01.T01:CDS 35.0%
! TATCCAAGATCTTTGCTATC+AGG - Chr6:223216-223235 None:intergenic 35.0%
! TGAATTGGCTGCTTCATTTT+GGG + Chr6:221697-221716 MsG0680030294.01.T01:CDS 35.0%
! TGGTCTTGGTTATTGTATTG+CGG + Chr6:222145-222164 MsG0680030294.01.T01:CDS 35.0%
! TTCAACAATGGTGGTGTATA+TGG + Chr6:223788-223807 MsG0680030294.01.T01:CDS 35.0%
! TTGAATTGGCTGCTTCATTT+TGG + Chr6:221696-221715 MsG0680030294.01.T01:CDS 35.0%
! TTTCTGCTTAGAACTTTTGC+TGG + Chr6:222816-222835 MsG0680030294.01.T01:intron 35.0%
!! AGAGATGCTATTTCGGAATT+TGG + Chr6:221999-222018 MsG0680030294.01.T01:CDS 35.0%
!! GAGCTTAGAAAACCGTTTTT+AGG + Chr6:221783-221802 MsG0680030294.01.T01:CDS 35.0%
!! TGGTGATGCTAAAGAAGTTT+TGG + Chr6:221812-221831 MsG0680030294.01.T01:CDS 35.0%
!!! AAACCGTTTTTAGGGTTAGT+TGG + Chr6:221792-221811 MsG0680030294.01.T01:CDS 35.0%
!!! AAAGGTTTTTTGGCATGCAT+TGG + Chr6:222545-222564 MsG0680030294.01.T01:intron 35.0%
AAGAAATTGGTGGAAAGGAC+AGG + Chr6:221549-221568 MsG0680030294.01.T01:CDS 40.0%
ACATCCTTTGTTCCAAATGC+AGG + Chr6:223869-223888 MsG0680030294.01.T01:CDS 40.0%
AGAAATTGGTGGAAAGGACA+GGG + Chr6:221550-221569 MsG0680030294.01.T01:CDS 40.0%
AGATACACATGAGATATGCG+TGG + Chr6:222977-222996 MsG0680030294.01.T01:intron 40.0%
AGATCCAAATAAACACCACC+AGG - Chr6:221315-221334 None:intergenic 40.0%
ATGGTAACATTTATGACGGC+TGG - Chr6:223995-224014 None:intergenic 40.0%
CAAAAGCTTCCATCATAGTG+TGG - Chr6:223898-223917 None:intergenic 40.0%
CATCCTTTGTTCCAAATGCA+GGG + Chr6:223870-223889 MsG0680030294.01.T01:CDS 40.0%
CATGGCTTATTCTCTGTTTC+AGG - Chr6:222743-222762 None:intergenic 40.0%
CCTTTCCCAAACACAATCAA+CGG - Chr6:221498-221517 None:intergenic 40.0%
CGTTGTGGAAAGCAATGAAA+CGG - Chr6:220984-221003 None:intergenic 40.0%
CTGAAACAGAGAATAAGCCA+TGG + Chr6:222741-222760 MsG0680030294.01.T01:intron 40.0%
CTTCTATAGTCTCAGGGAAA+AGG - Chr6:222702-222721 None:intergenic 40.0%
GCATCTCTGATAATCCTCAT+TGG - Chr6:221987-222006 None:intergenic 40.0%
GGTTAAAAGCGATTCAGCTT+CGG - Chr6:221373-221392 None:intergenic 40.0%
GTTCCTGATAGCAAAGATCT+TGG + Chr6:223210-223229 MsG0680030294.01.T01:intron 40.0%
GTTGTGATCCCTATTGGATT+TGG + Chr6:222319-222338 MsG0680030294.01.T01:intron 40.0%
TCGAGTAAAGTGAAGGACAA+TGG + Chr6:221906-221925 MsG0680030294.01.T01:CDS 40.0%
TCTCAGAATCTTTCTCTGCT+AGG + Chr6:223966-223985 MsG0680030294.01.T01:CDS 40.0%
TGAGCAGTGATGAATCTTAG+TGG + Chr6:222475-222494 MsG0680030294.01.T01:intron 40.0%
TGCTATTGGAAAATGTGACG+TGG + Chr6:221653-221672 MsG0680030294.01.T01:CDS 40.0%
TTAGGATGAAGAGTTGTAGG+TGG + Chr6:224268-224287 MsG0680030294.01.T01:three_prime_UTR 40.0%
TTCTAACGGAGAGACTCTTT+GGG + Chr6:220596-220615 MsG0680030294.01.T01:five_prime_UTR 40.0%
TTGAAAACAATCACCACCAC+AGG - Chr6:223773-223792 None:intergenic 40.0%
! AACAATGGTGGTGTATATGG+TGG + Chr6:223791-223810 MsG0680030294.01.T01:CDS 40.0%
! AAGATGATCCGTTTTGCTTG+GGG + Chr6:221856-221875 MsG0680030294.01.T01:CDS 40.0%
! AGAGCTGACTTTAGTTCATC+AGG - Chr6:223947-223966 None:intergenic 40.0%
! CTCTGCTAGATTTTTCGCTT+CGG - Chr6:221394-221413 None:intergenic 40.0%
! GAATTGGCTGCTTCATTTTG+GGG + Chr6:221698-221717 MsG0680030294.01.T01:CDS 40.0%
! GCATTTGGAACAAAGGATGT+CGG - Chr6:223869-223888 None:intergenic 40.0%
! TGCGACGCAATTTGGAATTT+CGG - Chr6:221262-221281 None:intergenic 40.0%
!! AAAGGGTATTTGGCATGCAT+TGG + Chr6:222450-222469 MsG0680030294.01.T01:intron 40.0%
!! AATGAGAGGTTGGCCTTTAA+AGG - Chr6:220715-220734 None:intergenic 40.0%
!! ACTATGATGGAAGCTTTTGG+TGG + Chr6:223899-223918 MsG0680030294.01.T01:CDS 40.0%
!! AGTTGGTGAATGTGATGTGA+GGG + Chr6:220528-220547 MsG0680030294.01.T01:five_prime_UTR 40.0%
!! CACACTATGATGGAAGCTTT+TGG + Chr6:223896-223915 MsG0680030294.01.T01:CDS 40.0%
!! CTATGATGGAAGCTTTTGGT+GGG + Chr6:223900-223919 MsG0680030294.01.T01:CDS 40.0%
!! GATGCTATTTCGGAATTTGG+AGG + Chr6:222002-222021 MsG0680030294.01.T01:CDS 40.0%
!! TAGTTGGTGAATGTGATGTG+AGG + Chr6:220527-220546 MsG0680030294.01.T01:five_prime_UTR 40.0%
!! TTCTGAAGGTGCTAATACCA+TGG + Chr6:222046-222065 MsG0680030294.01.T01:CDS 40.0%
!!! CTCTGGAGCCTTGTTATTTT+AGG + Chr6:223042-223061 MsG0680030294.01.T01:intron 40.0%
!!! TTTTTTGCAGCAGCATACCA+TGG - Chr6:222761-222780 None:intergenic 40.0%
AATTCCAAATTGCGTCGCAC+AGG + Chr6:221263-221282 MsG0680030294.01.T01:CDS 45.0%
ATAGTGTGGTACCCTGCATT+TGG - Chr6:223884-223903 None:intergenic 45.0%
GAAACATGGACCTCACAAGA+AGG + Chr6:223835-223854 MsG0680030294.01.T01:CDS 45.0%
GAACCAGAGATCATGACAGT+AGG - Chr6:221138-221157 None:intergenic 45.0%
GAACCAGTGTCATGTGTAAC+TGG + Chr6:220553-220572 MsG0680030294.01.T01:five_prime_UTR 45.0%
GAAGGACAATGGTGTGAAGA+TGG + Chr6:221917-221936 MsG0680030294.01.T01:CDS 45.0%
GATTCGCAACGGAATTAGCA+AGG - Chr6:221290-221309 None:intergenic 45.0%
GGATGTGAAGTTTGTGTTGG+TGG + Chr6:221740-221759 MsG0680030294.01.T01:CDS 45.0%
GGTGCTAATACCATGGATGT+TGG + Chr6:222053-222072 MsG0680030294.01.T01:CDS 45.0%
GTGGGAAGGGTTATCTTGTT+GGG + Chr6:223918-223937 MsG0680030294.01.T01:CDS 45.0%
GTTACCATTGATCCCTATGC+CGG + Chr6:224007-224026 MsG0680030294.01.T01:CDS 45.0%
GTTCTAACGGAGAGACTCTT+TGG + Chr6:220595-220614 MsG0680030294.01.T01:five_prime_UTR 45.0%
GTTGAAGGGGATTCTGGATA+TGG + Chr6:222203-222222 MsG0680030294.01.T01:CDS 45.0%
TGATTGGTGCGAGGTTGAAT+TGG + Chr6:221682-221701 MsG0680030294.01.T01:CDS 45.0%
TGCTAGCATTCACTTGGCAT+TGG - Chr6:224089-224108 None:intergenic 45.0%
TGTGAAGATGGAGGCTCAAT+TGG + Chr6:221929-221948 MsG0680030294.01.T01:CDS 45.0%
TGTTGCATTACAACTCCAGC+CGG - Chr6:224303-224322 None:intergenic 45.0%
TGTTGGATGGAATGAGAGGT+TGG - Chr6:220725-220744 None:intergenic 45.0%
TTGTGCGTTGTGATCCCTAT+TGG + Chr6:222313-222332 MsG0680030294.01.T01:intron 45.0%
! AGAGGCCGTTGATTGTGTTT+GGG + Chr6:221490-221509 MsG0680030294.01.T01:CDS 45.0%
! AGCTGTTTCGCTTCTGAGAA+AGG + Chr6:221464-221483 MsG0680030294.01.T01:CDS 45.0%
! ATGGAAGCTTTTGGTGGGAA+GGG + Chr6:223905-223924 MsG0680030294.01.T01:CDS 45.0%
! CCGTTGATTGTGTTTGGGAA+AGG + Chr6:221495-221514 MsG0680030294.01.T01:CDS 45.0%
! CTGAGTCAGCAAGGTTCTAA+CGG + Chr6:220582-220601 MsG0680030294.01.T01:five_prime_UTR 45.0%
! GTACCCTGCATTTGGAACAA+AGG - Chr6:223876-223895 None:intergenic 45.0%
! TAGGTTGGATGCTGGTACTT+GGG + Chr6:222109-222128 MsG0680030294.01.T01:CDS 45.0%
! TCGACCTGGTGGTGTTTATT+TGG + Chr6:221308-221327 MsG0680030294.01.T01:CDS 45.0%
! TGTTTCGCTTCTGAGAAAGG+CGG + Chr6:221467-221486 MsG0680030294.01.T01:CDS 45.0%
!! ACTTGGCATTGGCAAGTGAT+AGG - Chr6:224078-224097 None:intergenic 45.0%
!! ATTTTGGGGAAGAGCCGAAA+TGG + Chr6:221712-221731 MsG0680030294.01.T01:CDS 45.0%
!! TCGATCTGTGTTGGTTCAGA+AGG + Chr6:222076-222095 MsG0680030294.01.T01:CDS 45.0%
!! TTGAAAAAAAAAAAAAAAAA+AGG + Chr6:222426-222445 MsG0680030294.01.T01:intron 5.0%
AACCAAAACGATGTCGGTCG+CGG + Chr6:221162-221181 MsG0680030294.01.T01:CDS 50.0%
AATAGCAAGTGACCTAGCCG+CGG - Chr6:221640-221659 None:intergenic 50.0%
AATCCCTTCCCCATTGGAGT+CGG - Chr6:221588-221607 None:intergenic 50.0%
AATGGGGAAGGGATTGTTGC+CGG + Chr6:221593-221612 MsG0680030294.01.T01:CDS 50.0%
AATTCCGTTGCGAATCGACC+TGG + Chr6:221294-221313 MsG0680030294.01.T01:CDS 50.0%
AGAACCGGCATAGGGATCAA+TGG - Chr6:224014-224033 None:intergenic 50.0%
AGGTGGAATGATAAACCGGC+TGG + Chr6:224285-224304 MsG0680030294.01.T01:three_prime_UTR 50.0%
AGGTTGGATGCTGGTACTTG+GGG + Chr6:222110-222129 MsG0680030294.01.T01:CDS 50.0%
CACAGATCGACCAACATCCA+TGG - Chr6:222066-222085 None:intergenic 50.0%
CCACCAGTTACACATGACAC+TGG - Chr6:220559-220578 None:intergenic 50.0%
CCAGTGTCATGTGTAACTGG+TGG + Chr6:220556-220575 MsG0680030294.01.T01:five_prime_UTR 50.0%
CCCATTGGAGTCGGAAGAAA+GGG - Chr6:221579-221598 None:intergenic 50.0%
CCCTTTCTTCCGACTCCAAT+GGG + Chr6:221576-221595 MsG0680030294.01.T01:CDS 50.0%
CCTTTCTTCCGACTCCAATG+GGG + Chr6:221577-221596 MsG0680030294.01.T01:CDS 50.0%
CTCGAAATAGCTTCCACCCA+TGG - Chr6:221891-221910 None:intergenic 50.0%
GAAGGCTGCTAGCATTCACT+TGG - Chr6:224095-224114 None:intergenic 50.0%
GACAGTAGGCCATGTGTTAG+TGG - Chr6:221124-221143 None:intergenic 50.0%
GAGAGAAGGATTTAGCAGCG+AGG - Chr6:220882-220901 None:intergenic 50.0%
GATGGAGGCTCAATTGGCAA+AGG + Chr6:221935-221954 MsG0680030294.01.T01:CDS 50.0%
GGAAGAGCCGAAATGGTCTA+AGG + Chr6:221719-221738 MsG0680030294.01.T01:CDS 50.0%
GGACAATGGTGTGAAGATGG+AGG + Chr6:221920-221939 MsG0680030294.01.T01:CDS 50.0%
GGATGTTGGTCGATCTGTGT+TGG + Chr6:222067-222086 MsG0680030294.01.T01:CDS 50.0%
GGTGGGAAGGGTTATCTTGT+TGG + Chr6:223917-223936 MsG0680030294.01.T01:CDS 50.0%
GTGTCATGTGTAACTGGTGG+AGG + Chr6:220559-220578 MsG0680030294.01.T01:five_prime_UTR 50.0%
GTTTCTCTTCCGCGCTTCTA+CGG - Chr6:223820-223839 None:intergenic 50.0%
TCCCTTTCTTCCGACTCCAA+TGG + Chr6:221575-221594 MsG0680030294.01.T01:CDS 50.0%
TGAGTGCTCCCCAAGCAAAA+CGG - Chr6:221867-221886 None:intergenic 50.0%
TGCATTGGACTGAGCAGTGA+TGG + Chr6:222465-222484 MsG0680030294.01.T01:intron 50.0%
TGGAACAAAGGATGTCGGAG+CGG - Chr6:223864-223883 None:intergenic 50.0%
TGGCCTACTGTCATGATCTC+TGG + Chr6:221132-221151 MsG0680030294.01.T01:CDS 50.0%
TGGTGAATGTGATGTGAGGG+AGG + Chr6:220531-220550 MsG0680030294.01.T01:five_prime_UTR 50.0%
TGTGACGTGGCGATTGTGAT+TGG + Chr6:221666-221685 MsG0680030294.01.T01:CDS 50.0%
TTCAGAAGGAGCCAAGGACT+AGG + Chr6:222090-222109 MsG0680030294.01.T01:CDS 50.0%
TTCCACAACGAACAATCCGC+CGG + Chr6:220994-221013 MsG0680030294.01.T01:CDS 50.0%
TTCGGCGTCCAACACATGTT+CGG + Chr6:220904-220923 MsG0680030294.01.T01:CDS 50.0%
TTGGACGCCGAATTGAGAGA+AGG - Chr6:220896-220915 None:intergenic 50.0%
! AGAAGCGCGGAAGAGAAACA+TGG + Chr6:223821-223840 MsG0680030294.01.T01:CDS 50.0%
! CCTTGTCGTTGCTGTTGAAG+GGG + Chr6:222190-222209 MsG0680030294.01.T01:CDS 50.0%
! CTAGGTTGGATGCTGGTACT+TGG + Chr6:222108-222127 MsG0680030294.01.T01:CDS 50.0%
! GAGAGGCCGTTGATTGTGTT+TGG + Chr6:221489-221508 MsG0680030294.01.T01:CDS 50.0%
! GATGGAAGCTTTTGGTGGGA+AGG + Chr6:223904-223923 MsG0680030294.01.T01:CDS 50.0%
! GCCTTGTCGTTGCTGTTGAA+GGG + Chr6:222189-222208 MsG0680030294.01.T01:CDS 50.0%
! GTTGCTGTTGAAGGGGATTC+TGG + Chr6:222197-222216 MsG0680030294.01.T01:CDS 50.0%
! TGCAGGGTACCACACTATGA+TGG + Chr6:223886-223905 MsG0680030294.01.T01:CDS 50.0%
! TGTGTTGGTGGATGTGAGCA+AGG + Chr6:221752-221771 MsG0680030294.01.T01:CDS 50.0%
!! GAGAGGTTGGCCTTTAAAGG+AGG - Chr6:220712-220731 None:intergenic 50.0%
!! TGTTGGTTCAGAAGGAGCCA+AGG + Chr6:222084-222103 MsG0680030294.01.T01:CDS 50.0%
ACAGCAACGACAAGGCGATC+AGG - Chr6:222185-222204 None:intergenic 55.0%
AGGACCTGTGCGACGCAATT+TGG - Chr6:221270-221289 None:intergenic 55.0%
CAACACATGTTCGGCGTCGT+CGG + Chr6:220913-220932 MsG0680030294.01.T01:CDS 55.0%
CAATCACCACCACAGGCAGT+TGG - Chr6:223766-223785 None:intergenic 55.0%
CATCCTCCCACTCTCAGAAC+CGG - Chr6:224029-224048 None:intergenic 55.0%
CCAACGGCACCACTAACACA+TGG + Chr6:221112-221131 MsG0680030294.01.T01:CDS 55.0%
CCACTCTCAGAACCGGCATA+GGG - Chr6:224022-224041 None:intergenic 55.0%
CCAGCATCCAACCTAGTCCT+TGG - Chr6:222104-222123 None:intergenic 55.0%
CCCCATTGGAGTCGGAAGAA+AGG - Chr6:221580-221599 None:intergenic 55.0%
CCCCTTCAACAGCAACGACA+AGG - Chr6:222193-222212 None:intergenic 55.0%
CGTGTGGCGAGTGATGTTAC+GGG - Chr6:220943-220962 None:intergenic 55.0%
CTGGTACTTGGGGGACTATG+GGG + Chr6:222120-222139 MsG0680030294.01.T01:CDS 55.0%
CTTCCGACTCCAATGGGGAA+GGG + Chr6:221582-221601 MsG0680030294.01.T01:CDS 55.0%
GAAACGGATGCCGAGAGAGA+CGG - Chr6:220968-220987 None:intergenic 55.0%
GAACAAAGGATGTCGGAGCG+GGG - Chr6:223862-223881 None:intergenic 55.0%
GAAGGAGCCAAGGACTAGGT+TGG + Chr6:222094-222113 MsG0680030294.01.T01:CDS 55.0%
GCACCTTCAGAAACCACCAC+AGG - Chr6:222038-222057 None:intergenic 55.0%
GCAGCCAACGGATGATCATC+CGG - Chr6:221615-221634 None:intergenic 55.0%
GCGATCAGGATAAGCGACAG+TGG - Chr6:222171-222190 None:intergenic 55.0%
GCGTGTGGCGAGTGATGTTA+CGG - Chr6:220944-220963 None:intergenic 55.0%
GCTCCTGTGGTGGTTTCTGA+AGG + Chr6:222032-222051 MsG0680030294.01.T01:CDS 55.0%
GCTGGTACTTGGGGGACTAT+GGG + Chr6:222119-222138 MsG0680030294.01.T01:CDS 55.0%
GGAACAAAGGATGTCGGAGC+GGG - Chr6:223863-223882 None:intergenic 55.0%
GGCAACAATCCCTTCCCCAT+TGG - Chr6:221594-221613 None:intergenic 55.0%
GGTTGGATGCTGGTACTTGG+GGG + Chr6:222111-222130 MsG0680030294.01.T01:CDS 55.0%
GTGTGGCGAGTGATGTTACG+GGG - Chr6:220942-220961 None:intergenic 55.0%
GTTGCCGGATGATCATCCGT+TGG + Chr6:221608-221627 MsG0680030294.01.T01:CDS 55.0%
TCAGAAACCACCACAGGAGC+TGG - Chr6:222032-222051 None:intergenic 55.0%
TCTTCCGACTCCAATGGGGA+AGG + Chr6:221581-221600 MsG0680030294.01.T01:CDS 55.0%
TGCTGGTACTTGGGGGACTA+TGG + Chr6:222118-222137 MsG0680030294.01.T01:CDS 55.0%
TGGCGATTGTGATTGGTGCG+AGG + Chr6:221673-221692 MsG0680030294.01.T01:CDS 55.0%
TGGTACTTGGGGGACTATGG+GGG + Chr6:222121-222140 MsG0680030294.01.T01:CDS 55.0%
! CCAAGGACTAGGTTGGATGC+TGG + Chr6:222101-222120 MsG0680030294.01.T01:CDS 55.0%
! CGCCTTGTCGTTGCTGTTGA+AGG + Chr6:222188-222207 MsG0680030294.01.T01:CDS 55.0%
!! AACCGGCGGATTGTTCGTTG+TGG - Chr6:220999-221018 None:intergenic 55.0%
!! ACCACCAGGTCGATTCGCAA+CGG - Chr6:221301-221320 None:intergenic 55.0%
!! CCATGTGTTAGTGGTGCCGT+TGG - Chr6:221115-221134 None:intergenic 55.0%
!! CCTATGCCGGTTCTGAGAGT+GGG + Chr6:224020-224039 MsG0680030294.01.T01:CDS 55.0%
!! CTTGGGGAGCACTCATCCAT+GGG + Chr6:221872-221891 MsG0680030294.01.T01:CDS 55.0%
!! GCGGCTAGGTCACTTGCTAT+TGG + Chr6:221639-221658 MsG0680030294.01.T01:CDS 55.0%
ACTTGGGGGACTATGGGGGT+TGG + Chr6:222125-222144 MsG0680030294.01.T01:CDS 60.0%
AGGAAGTCCAGCTCCTGTGG+TGG + Chr6:222022-222041 MsG0680030294.01.T01:CDS 60.0%
CCCACTCTCAGAACCGGCAT+AGG - Chr6:224023-224042 None:intergenic 60.0%
CGACGACGCCGAACATGTGT+TGG - Chr6:220915-220934 None:intergenic 60.0%
GAGACGGTGAGGAAGATGCC+GGG - Chr6:221057-221076 None:intergenic 60.0%
GCTAGACCGTGAACGCAACC+GGG - Chr6:221084-221103 None:intergenic 60.0%
GGAGACGGTGAGGAAGATGC+CGG - Chr6:221058-221077 None:intergenic 60.0%
GGAGGTGAGGTAGCCGTAAG+CGG - Chr6:221031-221050 None:intergenic 60.0%
GGCATCTTCCTCACCGTCTC+CGG + Chr6:221057-221076 MsG0680030294.01.T01:CDS 60.0%
GGCTAGACCGTGAACGCAAC+CGG - Chr6:221085-221104 None:intergenic 60.0%
GGTCTAGCCGGTCTCTCCAA+CGG + Chr6:221096-221115 MsG0680030294.01.T01:CDS 60.0%
GGTTACGCTGCCTCCGCTTA+CGG + Chr6:221015-221034 MsG0680030294.01.T01:CDS 60.0%
GGTTGCGTTCACGGTCTAGC+CGG + Chr6:221084-221103 MsG0680030294.01.T01:CDS 60.0%
GTAAGCGGAGGCAGCGTAAC+CGG - Chr6:221016-221035 None:intergenic 60.0%
TCCGTTGCGAATCGACCTGG+TGG + Chr6:221297-221316 MsG0680030294.01.T01:CDS 60.0%
TCGGTACCAACTGCCTGTGG+TGG + Chr6:223757-223776 MsG0680030294.01.T01:CDS 60.0%
TGGAGGAAGTCCAGCTCCTG+TGG + Chr6:222019-222038 MsG0680030294.01.T01:CDS 60.0%
TGGTGGAGGCTGAGTCAGCA+AGG + Chr6:220573-220592 MsG0680030294.01.T01:five_prime_UTR 60.0%
! CACCGCGACCGACATCGTTT+TGG - Chr6:221167-221186 None:intergenic 60.0%
! CGCTTCTGAGAAAGGCGGAG+AGG + Chr6:221472-221491 MsG0680030294.01.T01:CDS 60.0%
! GGTTCGGTACCAACTGCCTG+TGG + Chr6:223754-223773 MsG0680030294.01.T01:CDS 60.0%
! TGGTGGTGACCGTAGAAGCG+CGG + Chr6:223808-223827 MsG0680030294.01.T01:CDS 60.0%
!! AGTGGTGCCGTTGGAGAGAC+CGG - Chr6:221106-221125 None:intergenic 60.0%
!! ATGCCGGTTCTGAGAGTGGG+AGG + Chr6:224023-224042 MsG0680030294.01.T01:CDS 60.0%
!! CCCTATGCCGGTTCTGAGAG+TGG + Chr6:224019-224038 MsG0680030294.01.T01:CDS 60.0%
!! GCTTGGGGAGCACTCATCCA+TGG + Chr6:221871-221890 MsG0680030294.01.T01:CDS 60.0%
!! GGGACTATGGGGGTTGGTCT+TGG + Chr6:222131-222150 MsG0680030294.01.T01:CDS 60.0%
AACGCAACCGGGACCGGAGA+CGG - Chr6:221073-221092 None:intergenic 65.0%
ACCGTGAACGCAACCGGGAC+CGG - Chr6:221079-221098 None:intergenic 65.0%
AGCAAGTGACCTAGCCGCGG+AGG - Chr6:221637-221656 None:intergenic 65.0%
CGGTGAGGAAGATGCCGGGA+CGG - Chr6:221053-221072 None:intergenic 65.0%
GGAGCGGGGTCCTTCTTGTG+AGG - Chr6:223848-223867 None:intergenic 65.0%
GGTGAGGAAGATGCCGGGAC+GGG - Chr6:221052-221071 None:intergenic 65.0%
GGTGAGGTAGCCGTAAGCGG+AGG - Chr6:221028-221047 None:intergenic 65.0%
TCCGGTCCCGGTTGCGTTCA+CGG + Chr6:221075-221094 MsG0680030294.01.T01:CDS 65.0%
TTCCTCACCGTCTCCGGTCC+CGG + Chr6:221063-221082 MsG0680030294.01.T01:CDS 65.0%
! TCATCCGTTGGCTGCCTCCG+CGG + Chr6:221620-221639 MsG0680030294.01.T01:CDS 65.0%
!! GGGGAGCACTCATCCATGGG+TGG + Chr6:221875-221894 MsG0680030294.01.T01:CDS 65.0%
AACCGGGACCGGAGACGGTG+AGG - Chr6:221068-221087 None:intergenic 70.0%
AGATGCCGGGACGGGAGGTG+AGG - Chr6:221044-221063 None:intergenic 70.0%
AGCGGAGGCAGCGTAACCGG+CGG - Chr6:221013-221032 None:intergenic 70.0%
CTAGCCGCGGAGGCAGCCAA+CGG - Chr6:221627-221646 None:intergenic 70.0%
GAGGAAGATGCCGGGACGGG+AGG - Chr6:221049-221068 None:intergenic 70.0%
GCCACACGCGCCGTCTCTCT+CGG + Chr6:220955-220974 MsG0680030294.01.T01:CDS 70.0%
GGCTACCTCACCTCCCGTCC+CGG + Chr6:221036-221055 MsG0680030294.01.T01:CDS 70.0%
GCCGAGAGAGACGGCGCGTG+TGG - Chr6:220959-220978 None:intergenic 75.0%
! CGTTGGCTGCCTCCGCGGCT+AGG + Chr6:221625-221644 MsG0680030294.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 220517 224419 220517 ID=MsG0680030294.01;Name=MsG0680030294.01
Chr6 mRNA 220517 224419 220517 ID=MsG0680030294.01.T01;Parent=MsG0680030294.01;Name=MsG0680030294.01.T01;_AED=0.20;_eAED=0.20;_QI=339|1|1|1|1|1|2|356|568
Chr6 exon 220517 222247 220517 ID=MsG0680030294.01.T01:exon:1910;Parent=MsG0680030294.01.T01
Chr6 exon 223749 224419 223749 ID=MsG0680030294.01.T01:exon:1911;Parent=MsG0680030294.01.T01
Chr6 five_prime_UTR 220517 220855 220517 ID=MsG0680030294.01.T01:five_prime_utr;Parent=MsG0680030294.01.T01
Chr6 CDS 220856 222247 220856 ID=MsG0680030294.01.T01:cds;Parent=MsG0680030294.01.T01
Chr6 CDS 223749 224063 223749 ID=MsG0680030294.01.T01:cds;Parent=MsG0680030294.01.T01
Chr6 three_prime_UTR 224064 224419 224064 ID=MsG0680030294.01.T01:three_prime_utr;Parent=MsG0680030294.01.T01
Gene Sequence

>MsG0680030294.01.T01

ATGGGAGAAATTGACGGTAATGTCCTCGCTGCTAAATCCTTCTCTCAATTCGGCGTCCAACACATGTTCGGCGTCGTCGGCATCCCCGTAACATCACTCGCCACACGCGCCGTCTCTCTCGGCATCCGTTTCATTGCTTTCCACAACGAACAATCCGCCGGTTACGCTGCCTCCGCTTACGGCTACCTCACCTCCCGTCCCGGCATCTTCCTCACCGTCTCCGGTCCCGGTTGCGTTCACGGTCTAGCCGGTCTCTCCAACGGCACCACTAACACATGGCCTACTGTCATGATCTCTGGTTCTTGTAACCAAAACGATGTCGGTCGCGGTGATTTTCAAGAACTCGATCAGATCGAAGCTGTTAAGCCTTTCACAAAATTCGCAATCAAAGCTAAACATATCTCCGAAATTCCAAATTGCGTCGCACAGGTCCTTGCTAATTCCGTTGCGAATCGACCTGGTGGTGTTTATTTGGATCTTCCTACTGATGTATTGCATCAGAAAGTTTCAGAATCCGAAGCTGAATCGCTTTTAACCGAAGCGAAAAATCTAGCAGAGAAAATTAAGAAAAGTCACGTTATAAGTATTGAATCTTCGAAGATTCAAGAAGCTGTTTCGCTTCTGAGAAAGGCGGAGAGGCCGTTGATTGTGTTTGGGAAAGGAGCAGCTTATGCTAAAGCTGAAGATGAATTGAAGAAATTGGTGGAAAGGACAGGGATTCCCTTTCTTCCGACTCCAATGGGGAAGGGATTGTTGCCGGATGATCATCCGTTGGCTGCCTCCGCGGCTAGGTCACTTGCTATTGGAAAATGTGACGTGGCGATTGTGATTGGTGCGAGGTTGAATTGGCTGCTTCATTTTGGGGAAGAGCCGAAATGGTCTAAGGATGTGAAGTTTGTGTTGGTGGATGTGAGCAAGGAAGAAATTGAGCTTAGAAAACCGTTTTTAGGGTTAGTTGGTGATGCTAAAGAAGTTTTGGAGATTTTGAATAAGGAGATTAAAGATGATCCGTTTTGCTTGGGGAGCACTCATCCATGGGTGGAAGCTATTTCGAGTAAAGTGAAGGACAATGGTGTGAAGATGGAGGCTCAATTGGCAAAGGACGTTGTGCCGTTTAATTTTTTGACGCCAATGAGGATTATCAGAGATGCTATTTCGGAATTTGGAGGAAGTCCAGCTCCTGTGGTGGTTTCTGAAGGTGCTAATACCATGGATGTTGGTCGATCTGTGTTGGTTCAGAAGGAGCCAAGGACTAGGTTGGATGCTGGTACTTGGGGGACTATGGGGGTTGGTCTTGGTTATTGTATTGCGGCCACTGTCGCTTATCCTGATCGCCTTGTCGTTGCTGTTGAAGGGGATTCTGGATATGGATTTAGTGCTATGGAAGTTGAGACATTGGTTCGGTACCAACTGCCTGTGGTGGTGATTGTTTTCAACAATGGTGGTGTATATGGTGGTGACCGTAGAAGCGCGGAAGAGAAACATGGACCTCACAAGAAGGACCCCGCTCCGACATCCTTTGTTCCAAATGCAGGGTACCACACTATGATGGAAGCTTTTGGTGGGAAGGGTTATCTTGTTGGGACACCTGATGAACTAAAGTCAGCTCTCTCAGAATCTTTCTCTGCTAGGAAACCAGCCGTCATAAATGTTACCATTGATCCCTATGCCGGTTCTGAGAGTGGGAGGATGCAACACAAGAATTGA
Protein sequence

>MsG0680030294.01.T01

MGEIDGNVLAAKSFSQFGVQHMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLTSRPGIFLTVSGPGCVHGLAGLSNGTTNTWPTVMISGSCNQNDVGRGDFQELDQIEAVKPFTKFAIKAKHISEIPNCVAQVLANSVANRPGGVYLDLPTDVLHQKVSESEAESLLTEAKNLAEKIKKSHVISIESSKIQEAVSLLRKAERPLIVFGKGAAYAKAEDELKKLVERTGIPFLPTPMGKGLLPDDHPLAASAARSLAIGKCDVAIVIGARLNWLLHFGEEPKWSKDVKFVLVDVSKEEIELRKPFLGLVGDAKEVLEILNKEIKDDPFCLGSTHPWVEAISSKVKDNGVKMEAQLAKDVVPFNFLTPMRIIRDAISEFGGSPAPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGTMGVGLGYCIAATVAYPDRLVVAVEGDSGYGFSAMEVETLVRYQLPVVVIVFNNGGVYGGDRRSAEEKHGPHKKDPAPTSFVPNAGYHTMMEAFGGKGYLVGTPDELKSALSESFSARKPAVINVTIDPYAGSESGRMQHKN*