AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030396.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030396.01.T01 MTR_6g005460 94.970 338 16 1 8 344 631 968 0.0 606
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030396.01.T01 AT5G14580 41.322 363 171 9 8 345 639 984 1.88e-68 232

Find 87 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGATGATAGGGTAAAAT+TGG 0.226491 6:-1654381 MsG0680030396.01.T01:CDS
ATCCATTACAGATTGATTCT+TGG 0.289491 6:+1655382 None:intergenic
GTCACCTCTATAAAAGAATA+TGG 0.312047 6:-1654971 MsG0680030396.01.T01:CDS
CCAACAGTTGGGCACCAGTT+TGG 0.333895 6:-1654589 MsG0680030396.01.T01:CDS
GTATTACAATTACTGGTGTT+TGG 0.344421 6:+1654477 None:intergenic
TATTACAATTACTGGTGTTT+GGG 0.354658 6:+1654478 None:intergenic
AGAGACTATGTCTGAAATCT+TGG 0.366226 6:+1654757 None:intergenic
CAACAGTTGGGCACCAGTTT+GGG 0.374325 6:-1654588 MsG0680030396.01.T01:CDS
ACATGGGTTAGTGTTAGATT+TGG 0.379311 6:-1654120 MsG0680030396.01.T01:CDS
AGGGGTAACCTTGGCGCAAC+TGG 0.392701 6:-1656604 MsG0680030396.01.T01:intron
ATTTCAGACATAGTCTCTAT+TGG 0.393834 6:-1654752 MsG0680030396.01.T01:CDS
GCACTGCGTATTACAATTAC+TGG 0.394724 6:+1654470 None:intergenic
GCAGGGCCTTCTCCACATTT+CGG 0.395372 6:-1654921 MsG0680030396.01.T01:CDS
CATGGGTTAGTGTTAGATTT+GGG 0.395389 6:-1654119 MsG0680030396.01.T01:CDS
CAGCTTTCTTTGATGTGCAT+TGG 0.395578 6:-1654725 MsG0680030396.01.T01:CDS
TTGTCAGGTTGTTTCTATAG+TGG 0.408871 6:-1655032 MsG0680030396.01.T01:intron
ATGGTCCTCTCATTGATGAT+AGG 0.414973 6:-1654391 MsG0680030396.01.T01:CDS
ATCTTGGTGGTCCATGTCCA+TGG 0.415317 6:+1653511 None:intergenic
TACCTCCAACTTCAATTTCA+CGG 0.417543 6:+1655008 None:intergenic
TAATGTTACCATGAACATCT+TGG 0.425440 6:+1654702 None:intergenic
TTCGGAGTTATCACATGAAC+CGG 0.430847 6:-1654903 MsG0680030396.01.T01:intron
CTTCCAGGCCATCATCTTGG+TGG 0.435214 6:+1653498 None:intergenic
ATAATTAGTCTCTGATCCTC+CGG 0.435612 6:+1654649 None:intergenic
TACTGGTGTTTGGGAGGCTG+AGG 0.439432 6:+1654487 None:intergenic
TTTCTTCCAGGCCATCATCT+TGG 0.439908 6:+1653495 None:intergenic
TGTCAGGTTGTTTCTATAGT+GGG 0.454979 6:-1655031 MsG0680030396.01.T01:intron
TTAGTGTTAGATTTGGGTGG+AGG 0.457807 6:-1654113 MsG0680030396.01.T01:CDS
TGAATTTAATGGTGGCAAGC+AGG 0.458285 6:-1654939 MsG0680030396.01.T01:CDS
GATCACAATCAAACTTCAAA+TGG 0.459164 6:-1654410 MsG0680030396.01.T01:CDS
AATGGATAAAACACTAGAAA+AGG 0.480985 6:-1655366 MsG0680030396.01.T01:intron
TACCAAGAATCAATCTGTAA+TGG 0.481885 6:-1655384 MsG0680030396.01.T01:CDS
AAAGCGGCTTTGCGTGGTCC+CGG 0.482324 6:-1654668 MsG0680030396.01.T01:CDS
ACCACGCAAAGCCGCTTTGA+GGG 0.484977 6:+1654673 None:intergenic
GATTTGGGTGGAGGAGTTCG+AGG 0.487837 6:-1654104 MsG0680030396.01.T01:CDS
TGGTCCTCTCATTGATGATA+GGG 0.494395 6:-1654390 MsG0680030396.01.T01:CDS
ATACTAGACGTGTCCCAAAC+TGG 0.499017 6:+1654575 None:intergenic
TTCCATCTCCTACAGACAGC+CGG 0.499670 6:+1655420 None:intergenic
GACCACGCAAAGCCGCTTTG+AGG 0.503721 6:+1654672 None:intergenic
GAATTTAATGGTGGCAAGCA+GGG 0.508483 6:-1654938 MsG0680030396.01.T01:CDS
TTAAGTTATTTATCATCCAC+AGG 0.510536 6:+1653559 None:intergenic
TGCACATCAAAGAAAGCTGC+TGG 0.521327 6:+1654729 None:intergenic
GGCAAGGGACCTGACTGAGA+TGG 0.524425 6:+1654296 None:intergenic
ACTGCAAAAGATTTGCCACT+TGG 0.529258 6:-1654179 MsG0680030396.01.T01:CDS
TCTGATACTCAAAGGGCAGA+AGG 0.529983 6:-1654236 MsG0680030396.01.T01:CDS
TGCATTGGCCAAGATGTTCA+TGG 0.530439 6:-1654710 MsG0680030396.01.T01:CDS
TACAATTACTGGTGTTTGGG+AGG 0.534132 6:+1654481 None:intergenic
GTGGGCCGTGAAATTGAAGT+TGG 0.534404 6:-1655013 MsG0680030396.01.T01:CDS
CAAAAGTAACTACCAACAGT+TGG 0.537176 6:-1654601 MsG0680030396.01.T01:CDS
ATTATCTACAATCAGGTGCC+CGG 0.538326 6:-1655439 MsG0680030396.01.T01:intron
ATTGATTCTCCATCTCAGTC+AGG 0.541945 6:-1654305 MsG0680030396.01.T01:CDS
GCCCGGCTGTCTGTAGGAGA+TGG 0.542067 6:-1655422 MsG0680030396.01.T01:CDS
GTCGAAATCTGATACTCAAA+GGG 0.542952 6:-1654243 MsG0680030396.01.T01:CDS
AGTCGAAATCTGATACTCAA+AGG 0.543620 6:-1654244 MsG0680030396.01.T01:CDS
CGTGAGACAGCCCATGGACA+TGG 0.543974 6:-1653522 MsG0680030396.01.T01:CDS
AGATGGGAAAACGGACTTGA+AGG 0.546430 6:-1653643 MsG0680030396.01.T01:CDS
TTCTTTCGTGAGACAGCCCA+TGG 0.546433 6:-1653528 MsG0680030396.01.T01:CDS
GACATGGACCACCAAGATGA+TGG 0.550954 6:-1653506 None:intergenic
ATCATCCACAGGAACACTAC+CGG 0.552189 6:+1653570 None:intergenic
GGGTTAGTGTTAGATTTGGG+TGG 0.554630 6:-1654116 MsG0680030396.01.T01:CDS
TTTGCAGGGAGATGGGAAAA+CGG 0.555905 6:-1653652 MsG0680030396.01.T01:intron
AAGATGACTCTACCTCAAAA+CGG 0.557176 6:+1654075 None:intergenic
AAAAGTAACTACCAACAGTT+GGG 0.557723 6:-1654600 MsG0680030396.01.T01:CDS
TCTTGGTGGTCCATGTCCAT+GGG 0.562390 6:+1653512 None:intergenic
TGCAGATGTGGCAGATACAT+CGG 0.567491 6:+1654622 None:intergenic
TAATTAGTCTCTGATCCTCC+GGG 0.568415 6:+1654650 None:intergenic
TCCCTCAAAGCGGCTTTGCG+TGG 0.570819 6:-1654674 MsG0680030396.01.T01:CDS
CTGATACTCAAAGGGCAGAA+GGG 0.572105 6:-1654235 MsG0680030396.01.T01:CDS
GGAGGAGAAATCTATCAGCT+TGG 0.573621 6:-1654432 MsG0680030396.01.T01:CDS
TCAGGTGCCCGGCTGTCTGT+AGG 0.574924 6:-1655428 MsG0680030396.01.T01:intron
TCCATCTCCTACAGACAGCC+GGG 0.579815 6:+1655421 None:intergenic
GGCCGTGAAATTGAAGTTGG+AGG 0.582532 6:-1655010 MsG0680030396.01.T01:CDS
ACTGGTGTTTGGGAGGCTGA+GGG 0.585616 6:+1654488 None:intergenic
CAACTTTACCAGTTGCGCCA+AGG 0.585916 6:+1656596 None:intergenic
AAATGTATGAGGGGTAACCT+TGG 0.589354 6:-1656613 None:intergenic
GGGAAAACGGACTTGAAGGT+AGG 0.615118 6:-1653639 MsG0680030396.01.T01:CDS
ATAACTCCGAAATGTGGAGA+AGG 0.623304 6:+1654915 None:intergenic
GAATTCCGGTAGTGTTCCTG+TGG 0.627399 6:-1653575 MsG0680030396.01.T01:CDS
CCAAACTGGTGCCCAACTGT+TGG 0.628365 6:+1654589 None:intergenic
TGCTGCAGAATGTGATGAGG+AGG 0.635597 6:-1654450 MsG0680030396.01.T01:CDS
TTCACTAGCAAATGTATGAG+GGG 0.636078 6:-1656622 None:intergenic
GAAGTTGGAGGTATCTACAA+AGG 0.637377 6:-1654998 MsG0680030396.01.T01:CDS
GCGGCTTTGCGTGGTCCCGG+AGG 0.638353 6:-1654665 MsG0680030396.01.T01:CDS
CATTAAATTATCCCTCAAAG+CGG 0.641563 6:-1654684 MsG0680030396.01.T01:CDS
CATGTGATAACTCCGAAATG+TGG 0.647504 6:+1654909 None:intergenic
TTTACCCTATCATCAATGAG+AGG 0.649663 6:+1654386 None:intergenic
CAGTGCTGCAGAATGTGATG+AGG 0.715070 6:-1654453 MsG0680030396.01.T01:CDS
ATTGCATATTGAGATTTACG+AGG 0.730462 6:+1654326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTAATATCTTTTAAC+AGG + Chr6:1654493-1654512 None:intergenic 10.0%
!!! TAAATAATATTAACCTATTT+TGG - Chr6:1654302-1654321 MsG0680030396.01.T01:CDS 10.0%
!! ATCATAAGTAAAAAAATGTT+TGG + Chr6:1656131-1656150 None:intergenic 15.0%
!! TTGATTATTTATAGCAAAAA+TGG + Chr6:1656325-1656344 None:intergenic 15.0%
!!! ATTAAAATATTGTCCTTTTT+TGG - Chr6:1654503-1654522 MsG0680030396.01.T01:CDS 15.0%
!!! TAGAGAATATGAATAATTTT+TGG + Chr6:1656204-1656223 None:intergenic 15.0%
!! CAATAAACCATACAAAAAAA+TGG + Chr6:1656172-1656191 None:intergenic 20.0%
!! GTAAGAATTTAAGAAGTTAT+AGG + Chr6:1656370-1656389 None:intergenic 20.0%
!! TTAAAATAACAGACAGAAAA+GGG + Chr6:1655049-1655068 None:intergenic 20.0%
!!! AATACGTTTGAAATTGTATT+TGG + Chr6:1654242-1654261 None:intergenic 20.0%
!!! ATACAATTTTATAGGAAGAA+GGG - Chr6:1654374-1654393 MsG0680030396.01.T01:CDS 20.0%
!!! ATACGTTTGAAATTGTATTT+GGG + Chr6:1654241-1654260 None:intergenic 20.0%
!!! TACAAATCCATTTTTTTGTA+TGG - Chr6:1656162-1656181 MsG0680030396.01.T01:intron 20.0%
! AAAAAAATGTTTGGCAAAAG+AGG + Chr6:1656122-1656141 None:intergenic 25.0%
! AAACTTTCAAAACAAGATCA+AGG + Chr6:1654440-1654459 None:intergenic 25.0%
! AAATAGGTGTATATATGCAA+TGG - Chr6:1654169-1654188 MsG0680030396.01.T01:CDS 25.0%
! AATGGATAAAACACTAGAAA+AGG - Chr6:1654750-1654769 MsG0680030396.01.T01:CDS 25.0%
! AATTATTACGTCATACATAC+CGG + Chr6:1655235-1655254 None:intergenic 25.0%
! ATATATACACACATAGTTTG+CGG - Chr6:1654613-1654632 MsG0680030396.01.T01:CDS 25.0%
! CAAGACATATAATATTAGTG+AGG + Chr6:1653870-1653889 None:intergenic 25.0%
! CAGTTTCTTTATGATATGTA+TGG - Chr6:1656080-1656099 MsG0680030396.01.T01:intron 25.0%
! CTTAAAATAACAGACAGAAA+AGG + Chr6:1655050-1655069 None:intergenic 25.0%
! TATTACAATTACTGGTGTTT+GGG + Chr6:1655641-1655660 None:intergenic 25.0%
! TCATCTTTATGTTTCATAAG+TGG - Chr6:1656057-1656076 MsG0680030396.01.T01:intron 25.0%
! TCATTTATAAGTAAAGTGCA+AGG + Chr6:1654266-1654285 None:intergenic 25.0%
! TCTTCCTATAAAATTGTATC+AGG + Chr6:1654373-1654392 None:intergenic 25.0%
! TGCTATTATTATCTACAATC+AGG - Chr6:1654670-1654689 MsG0680030396.01.T01:CDS 25.0%
! TGTTACATACAATTTAACGA+TGG + Chr6:1656404-1656423 None:intergenic 25.0%
! TTAAGTTATTTATCATCCAC+AGG + Chr6:1656560-1656579 None:intergenic 25.0%
! TTGACAAACTTCAAAATAGA+AGG - Chr6:1654193-1654212 MsG0680030396.01.T01:CDS 25.0%
!! ACATTTGCTAGTGAAAAAAA+AGG + Chr6:1653690-1653709 None:intergenic 25.0%
!! CATTTGCTAGTGAAAAAAAA+GGG + Chr6:1653689-1653708 None:intergenic 25.0%
!! CTTTTCAATGTAATGAACTA+TGG - Chr6:1653983-1654002 MsG0680030396.01.T01:intron 25.0%
!! GATACAATTTTATAGGAAGA+AGG - Chr6:1654373-1654392 MsG0680030396.01.T01:CDS 25.0%
!! TATTATTGTTCTGTTTTGCA+GGG - Chr6:1656450-1656469 MsG0680030396.01.T01:intron 25.0%
!! TCAAAATAGAAGGTGTATAA+AGG - Chr6:1654203-1654222 MsG0680030396.01.T01:CDS 25.0%
!! TTTTCACTAGCAAATGTATA+TGG - Chr6:1653693-1653712 MsG0680030396.01.T01:intron 25.0%
!!! ATATTAGTGAGGCATTTTTA+AGG + Chr6:1653859-1653878 None:intergenic 25.0%
!!! ATTTTTTTCTATAGGCAACT+CGG + Chr6:1655590-1655609 None:intergenic 25.0%
!!! TATTAGTGAGGCATTTTTAA+GGG + Chr6:1653858-1653877 None:intergenic 25.0%
AAAAACTCTATGCTTTCCTT+AGG - Chr6:1655333-1655352 MsG0680030396.01.T01:intron 30.0%
AAAACTATCTTGATCCCAAA+AGG + Chr6:1653807-1653826 None:intergenic 30.0%
AAAAGGAATCTATATGCTTG+AGG + Chr6:1653790-1653809 None:intergenic 30.0%
AAAAGTAACTACCAACAGTT+GGG - Chr6:1655516-1655535 MsG0680030396.01.T01:intron 30.0%
AAAATGTGTGCTTACATGTT+TGG + Chr6:1653957-1653976 None:intergenic 30.0%
AATGAACTATGGTTAAATCG+CGG - Chr6:1653994-1654013 MsG0680030396.01.T01:intron 30.0%
AGAACAATAATAGGTCAGTA+AGG + Chr6:1656442-1656461 None:intergenic 30.0%
ATTGCATATTGAGATTTACG+AGG + Chr6:1655793-1655812 None:intergenic 30.0%
ATTTCAGACATAGTCTCTAT+TGG - Chr6:1655364-1655383 MsG0680030396.01.T01:intron 30.0%
CATTAAATTATCCCTCAAAG+CGG - Chr6:1655432-1655451 MsG0680030396.01.T01:intron 30.0%
CCTATAGAAAAAAATGAGGT+GGG - Chr6:1655595-1655614 MsG0680030396.01.T01:intron 30.0%
CTGCAAAACAGAACAATAAT+AGG + Chr6:1656451-1656470 None:intergenic 30.0%
CTGCTTTGTAAATTGTATTC+TGG - Chr6:1654345-1654364 MsG0680030396.01.T01:CDS 30.0%
CTTCAAAATAGGTGTATTGA+AGG + Chr6:1653730-1653749 None:intergenic 30.0%
GAACAATAATAGGTCAGTAA+GGG + Chr6:1656441-1656460 None:intergenic 30.0%
GAATACAATTTACAAAGCAG+AGG + Chr6:1654345-1654364 None:intergenic 30.0%
GATCACAATCAAACTTCAAA+TGG - Chr6:1655706-1655725 MsG0680030396.01.T01:intron 30.0%
GTATTACAATTACTGGTGTT+TGG + Chr6:1655642-1655661 None:intergenic 30.0%
GTCACCTCTATAAAAGAATA+TGG - Chr6:1655145-1655164 MsG0680030396.01.T01:intron 30.0%
GTTGCCTATAGAAAAAAATG+AGG - Chr6:1655591-1655610 MsG0680030396.01.T01:intron 30.0%
TAATGTTACCATGAACATCT+TGG + Chr6:1655417-1655436 None:intergenic 30.0%
TACCAAGAATCAATCTGTAA+TGG - Chr6:1654732-1654751 MsG0680030396.01.T01:CDS 30.0%
TGCACGAATTTGAAAAACTT+TGG + Chr6:1655977-1655996 None:intergenic 30.0%
TGCATGATATTATCATCACA+TGG + Chr6:1654928-1654947 None:intergenic 30.0%
TGTTTGACAAACGTCAAAAT+AGG - Chr6:1654153-1654172 MsG0680030396.01.T01:CDS 30.0%
! AGCTCCATATTCTTTTATAG+AGG + Chr6:1655152-1655171 None:intergenic 30.0%
! ATCCATTACAGATTGATTCT+TGG + Chr6:1654737-1654756 None:intergenic 30.0%
! CAAGCATATAGATTCCTTTT+GGG - Chr6:1653790-1653809 MsG0680030396.01.T01:intron 30.0%
! CATTGATGATAGGGTAAAAT+TGG - Chr6:1655735-1655754 MsG0680030396.01.T01:intron 30.0%
! CTATTATTGTTCTGTTTTGC+AGG - Chr6:1656449-1656468 MsG0680030396.01.T01:intron 30.0%
! GCTTTTGTTGAATTTAATGG+TGG - Chr6:1655169-1655188 MsG0680030396.01.T01:intron 30.0%
! GGAGCTTTTGTTGAATTTAA+TGG - Chr6:1655166-1655185 MsG0680030396.01.T01:intron 30.0%
! GGTCAAGATTTTCAATGTAA+TGG + Chr6:1653915-1653934 None:intergenic 30.0%
! TCAAGCATATAGATTCCTTT+TGG - Chr6:1653789-1653808 MsG0680030396.01.T01:intron 30.0%
! TCTTTTTGCGTTGAATGTTT+TGG + Chr6:1655853-1655872 None:intergenic 30.0%
!! GCTTTAAATTTTGTTCCAAG+TGG + Chr6:1655955-1655974 None:intergenic 30.0%
!! TTTTTCAAATTCGTGCACAT+GGG - Chr6:1655980-1655999 MsG0680030396.01.T01:intron 30.0%
!!! CATGTTTTAGAAGCCAAAAA+AGG + Chr6:1654519-1654538 None:intergenic 30.0%
AAACAACCTGACAAACAAGA+AGG + Chr6:1655078-1655097 None:intergenic 35.0%
AAGATGACTCTACCTCAAAA+CGG + Chr6:1656044-1656063 None:intergenic 35.0%
AGCAAAAATGGAAATTGCGA+AGG + Chr6:1656313-1656332 None:intergenic 35.0%
AGTACAGTCCATTACATTGT+GGG + Chr6:1654102-1654121 None:intergenic 35.0%
AGTCGAAATCTGATACTCAA+AGG - Chr6:1655872-1655891 MsG0680030396.01.T01:intron 35.0%
ATGTTGAACCCACAATGTAA+TGG - Chr6:1654091-1654110 MsG0680030396.01.T01:CDS 35.0%
ATGTTTAATGGTTCATGCGA+TGG + Chr6:1653762-1653781 None:intergenic 35.0%
ATTTACAAAGCAGAGGAAGA+TGG + Chr6:1654338-1654357 None:intergenic 35.0%
CAAAAGTAACTACCAACAGT+TGG - Chr6:1655515-1655534 MsG0680030396.01.T01:intron 35.0%
CTTGAGGAAGCTATGTTTAA+TGG + Chr6:1653774-1653793 None:intergenic 35.0%
CTTTCAAAACAAGATCAAGG+AGG + Chr6:1654437-1654456 None:intergenic 35.0%
GCAAAAATGGAAATTGCGAA+GGG + Chr6:1656312-1656331 None:intergenic 35.0%
GCAAATGTATATGGATCACT+TGG - Chr6:1653702-1653721 MsG0680030396.01.T01:intron 35.0%
GCCTATAGAAAAAAATGAGG+TGG - Chr6:1655594-1655613 MsG0680030396.01.T01:intron 35.0%
GGTTGGACAAACTTCAAAAT+AGG + Chr6:1653741-1653760 None:intergenic 35.0%
GTCGAAATCTGATACTCAAA+GGG - Chr6:1655873-1655892 MsG0680030396.01.T01:intron 35.0%
GTGAATGATACCGTACAAAT+CGG + Chr6:1653834-1653853 None:intergenic 35.0%
TAATAGGTCAGTAAGGGAAT+TGG + Chr6:1656435-1656454 None:intergenic 35.0%
TACCTCCAACTTCAATTTCA+CGG + Chr6:1655111-1655130 None:intergenic 35.0%
TCTTGTGTAAAAACAGGGTA+AGG - Chr6:1653589-1653608 MsG0680030396.01.T01:CDS 35.0%
TGGAATCAAAAGGCCAAAAT+AGG + Chr6:1654318-1654337 None:intergenic 35.0%
TTTACCCTATCATCAATGAG+AGG + Chr6:1655733-1655752 None:intergenic 35.0%
! AGAGACTATGTCTGAAATCT+TGG + Chr6:1655362-1655381 None:intergenic 35.0%
! ATAATTAGTCTCTGATCCTC+CGG + Chr6:1655470-1655489 None:intergenic 35.0%
! GGCTCCTGATACAATTTTAT+AGG - Chr6:1654366-1654385 MsG0680030396.01.T01:CDS 35.0%
! TCTGAAATCTTGGAAACCTA+AGG + Chr6:1655352-1655371 None:intergenic 35.0%
!! AATGGACTACAATGTGATTG+CGG + Chr6:1653897-1653916 None:intergenic 35.0%
!! ACATGGGTTAGTGTTAGATT+TGG - Chr6:1655996-1656015 MsG0680030396.01.T01:intron 35.0%
!! AGTTTTCCATCCGATTTGTA+CGG - Chr6:1653821-1653840 MsG0680030396.01.T01:intron 35.0%
!! CATGGGTTAGTGTTAGATTT+GGG - Chr6:1655997-1656016 MsG0680030396.01.T01:intron 35.0%
!! GTTTTTCAAATTCGTGCACA+TGG - Chr6:1655979-1655998 MsG0680030396.01.T01:intron 35.0%
!! TGTCAGGTTGTTTCTATAGT+GGG - Chr6:1655085-1655104 MsG0680030396.01.T01:intron 35.0%
!! TTGTCAGGTTGTTTCTATAG+TGG - Chr6:1655084-1655103 MsG0680030396.01.T01:intron 35.0%
!!! AGATTTACGAGGTTTTGACT+TGG + Chr6:1655782-1655801 None:intergenic 35.0%
!!! CCCACCTCATTTTTTTCTAT+AGG + Chr6:1655598-1655617 None:intergenic 35.0%
!!! GTTGAATGTTTTGGCTTCTT+TGG + Chr6:1655844-1655863 None:intergenic 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG + Chr6:1653590-1653609 None:intergenic 35.0%
AATTGTATTTGGGCCACACT+TGG + Chr6:1654231-1654250 None:intergenic 40.0%
ACAGCCTCTTGTGTAAAAAC+AGG - Chr6:1653583-1653602 MsG0680030396.01.T01:CDS 40.0%
ACTGCAAAAGATTTGCCACT+TGG - Chr6:1655937-1655956 MsG0680030396.01.T01:intron 40.0%
AGACAAGACAACTGTTAGTG+TGG - Chr6:1654018-1654037 MsG0680030396.01.T01:intron 40.0%
AGTGAAAAAAAAGGGCAACC+CGG + Chr6:1653681-1653700 None:intergenic 40.0%
ATAACTCCGAAATGTGGAGA+AGG + Chr6:1655204-1655223 None:intergenic 40.0%
ATGATACCGTACAAATCGGA+TGG + Chr6:1653830-1653849 None:intergenic 40.0%
ATGGAATGTTGCATGCATGT+AGG - Chr6:1654054-1654073 MsG0680030396.01.T01:intron 40.0%
ATTATCTACAATCAGGTGCC+CGG - Chr6:1654677-1654696 MsG0680030396.01.T01:CDS 40.0%
CAGAGGAAGATGGAATCAAA+AGG + Chr6:1654328-1654347 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAACA+GGG - Chr6:1653584-1653603 MsG0680030396.01.T01:CDS 40.0%
CAGCTTTCTTTGATGTGCAT+TGG - Chr6:1655391-1655410 MsG0680030396.01.T01:CDS 40.0%
CATCACATGGATTTCTGATC+AGG + Chr6:1654915-1654934 None:intergenic 40.0%
CATGTGATAACTCCGAAATG+TGG + Chr6:1655210-1655229 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG - Chr6:1653615-1653634 MsG0680030396.01.T01:CDS 40.0%
CTGAGACATTTGTCTCACTT+AGG + Chr6:1654891-1654910 None:intergenic 40.0%
GAATTTAATGGTGGCAAGCA+GGG - Chr6:1655178-1655197 MsG0680030396.01.T01:intron 40.0%
GAGTACAGTCCATTACATTG+TGG + Chr6:1654103-1654122 None:intergenic 40.0%
GCAAAAGAGGTAAAAGACAG+GGG + Chr6:1656109-1656128 None:intergenic 40.0%
GCACTGCGTATTACAATTAC+TGG + Chr6:1655649-1655668 None:intergenic 40.0%
GGATTGCTGAAGCATAATTC+AGG + Chr6:1653936-1653955 None:intergenic 40.0%
GGCAAAAGAGGTAAAAGACA+GGG + Chr6:1656110-1656129 None:intergenic 40.0%
TACAATTACTGGTGTTTGGG+AGG + Chr6:1655638-1655657 None:intergenic 40.0%
TACATGCATGCAACATTCCA+TGG + Chr6:1654055-1654074 None:intergenic 40.0%
TGAATTTAATGGTGGCAAGC+AGG - Chr6:1655177-1655196 MsG0680030396.01.T01:intron 40.0%
TGGCAAAAGAGGTAAAAGAC+AGG + Chr6:1656111-1656130 None:intergenic 40.0%
TTAATGGTTCATGCGATGGT+TGG + Chr6:1653758-1653777 None:intergenic 40.0%
TTAGTGTTAGATTTGGGTGG+AGG - Chr6:1656003-1656022 MsG0680030396.01.T01:intron 40.0%
TTCGGAGTTATCACATGAAC+CGG - Chr6:1655213-1655232 MsG0680030396.01.T01:intron 40.0%
! ATGGTCCTCTCATTGATGAT+AGG - Chr6:1655725-1655744 MsG0680030396.01.T01:intron 40.0%
! ATTGATTCTCCATCTCAGTC+AGG - Chr6:1655811-1655830 MsG0680030396.01.T01:intron 40.0%
! GTTCAACCTTCTTGTTTGTC+AGG - Chr6:1655069-1655088 MsG0680030396.01.T01:intron 40.0%
! TAATTAGTCTCTGATCCTCC+GGG + Chr6:1655469-1655488 None:intergenic 40.0%
! TGGTCCTCTCATTGATGATA+GGG - Chr6:1655726-1655745 MsG0680030396.01.T01:intron 40.0%
!! CTCTCAAGCTTTTCTAGCAA+GGG - Chr6:1656519-1656538 MsG0680030396.01.T01:intron 40.0%
!! CTTTTCTAGCAAGGGAATTC+CGG - Chr6:1656527-1656546 MsG0680030396.01.T01:intron 40.0%
!! GAAGTTGGAGGTATCTACAA+AGG - Chr6:1655118-1655137 MsG0680030396.01.T01:intron 40.0%
!!! ATGTTTTGGCTTCTTTGGCA+AGG + Chr6:1655839-1655858 None:intergenic 40.0%
!!! GGTAGTTACTTTTGCAGATG+TGG + Chr6:1655509-1655528 None:intergenic 40.0%
!!! GTGTGGCATTTTTAGAACCA+TGG - Chr6:1654035-1654054 MsG0680030396.01.T01:intron 40.0%
!!! TGTTTTGGCTTCTTTGGCAA+GGG + Chr6:1655838-1655857 None:intergenic 40.0%
!!! TTACGAGGTTTTGACTTGGA+TGG + Chr6:1655778-1655797 None:intergenic 40.0%
AAGCGAGACACTAAACTGTG+TGG + Chr6:1656287-1656306 None:intergenic 45.0%
AGGTCAGTAAGGGAATTGGA+TGG + Chr6:1656431-1656450 None:intergenic 45.0%
ATACTAGACGTGTCCCAAAC+TGG + Chr6:1655544-1655563 None:intergenic 45.0%
ATCATCCACAGGAACACTAC+CGG + Chr6:1656549-1656568 None:intergenic 45.0%
CTGATACTCAAAGGGCAGAA+GGG - Chr6:1655881-1655900 MsG0680030396.01.T01:intron 45.0%
GCGTACAATACACCAAATGG+TGG - Chr6:1653614-1653633 MsG0680030396.01.T01:CDS 45.0%
GCTGCGTACAATACACCAAA+TGG - Chr6:1653611-1653630 MsG0680030396.01.T01:CDS 45.0%
GGAGGAGAAATCTATCAGCT+TGG - Chr6:1655684-1655703 MsG0680030396.01.T01:intron 45.0%
GTGTATAAAGGCTCCAAGTG+TGG - Chr6:1654215-1654234 MsG0680030396.01.T01:CDS 45.0%
GTTGTTGTCATGTGACCGTA+AGG - Chr6:1653539-1653558 MsG0680030396.01.T01:CDS 45.0%
TCTGATACTCAAAGGGCAGA+AGG - Chr6:1655880-1655899 MsG0680030396.01.T01:intron 45.0%
TGCACATCAAAGAAAGCTGC+TGG + Chr6:1655390-1655409 None:intergenic 45.0%
TGCAGATGTGGCAGATACAT+CGG + Chr6:1655497-1655516 None:intergenic 45.0%
TTTGCAGGGAGATGGGAAAA+CGG - Chr6:1656464-1656483 MsG0680030396.01.T01:intron 45.0%
! AGATGGGAAAACGGACTTGA+AGG - Chr6:1656473-1656492 MsG0680030396.01.T01:intron 45.0%
! GGGTTAGTGTTAGATTTGGG+TGG - Chr6:1656000-1656019 MsG0680030396.01.T01:intron 45.0%
! GTTCTGTTTTGCAGGGAGAT+GGG - Chr6:1656457-1656476 MsG0680030396.01.T01:intron 45.0%
! TCGAGGAATGTACCGTTTTG+AGG - Chr6:1656029-1656048 MsG0680030396.01.T01:intron 45.0%
! TGCATTGGCCAAGATGTTCA+TGG - Chr6:1655406-1655425 MsG0680030396.01.T01:CDS 45.0%
! TGTTCTGTTTTGCAGGGAGA+TGG - Chr6:1656456-1656475 MsG0680030396.01.T01:intron 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + Chr6:1653579-1653598 None:intergenic 45.0%
!! GCTCTCAAGCTTTTCTAGCA+AGG - Chr6:1656518-1656537 MsG0680030396.01.T01:intron 45.0%
CAACAGTTGGGCACCAGTTT+GGG - Chr6:1655528-1655547 MsG0680030396.01.T01:intron 50.0%
CAACTTTACCAGTTGCGCCA+AGG + Chr6:1653523-1653542 None:intergenic 50.0%
CAAGGAGGATCAGAACTAGC+AGG + Chr6:1654422-1654441 None:intergenic 50.0%
CAGTGCTGCAGAATGTGATG+AGG - Chr6:1655663-1655682 MsG0680030396.01.T01:intron 50.0%
GAATTCCGGTAGTGTTCCTG+TGG - Chr6:1656541-1656560 MsG0680030396.01.T01:intron 50.0%
GACTTGAACCCGTGACCTTA+CGG + Chr6:1653557-1653576 None:intergenic 50.0%
GGCCGTGAAATTGAAGTTGG+AGG - Chr6:1655106-1655125 MsG0680030396.01.T01:intron 50.0%
GTCATGTGACCGTAAGGTCA+CGG - Chr6:1653545-1653564 MsG0680030396.01.T01:CDS 50.0%
GTGGGCCGTGAAATTGAAGT+TGG - Chr6:1655103-1655122 MsG0680030396.01.T01:intron 50.0%
TCATGTGACCGTAAGGTCAC+GGG - Chr6:1653546-1653565 MsG0680030396.01.T01:CDS 50.0%
TGCTGCAGAATGTGATGAGG+AGG - Chr6:1655666-1655685 MsG0680030396.01.T01:intron 50.0%
TTCCATCTCCTACAGACAGC+CGG + Chr6:1654699-1654718 None:intergenic 50.0%
TTCTTTCGTGAGACAGCCCA+TGG - Chr6:1656588-1656607 MsG0680030396.01.T01:intron 50.0%
! ATCTTGGTGGTCCATGTCCA+TGG + Chr6:1656608-1656627 None:intergenic 50.0%
! GGGAAAACGGACTTGAAGGT+AGG - Chr6:1656477-1656496 MsG0680030396.01.T01:intron 50.0%
! TCTTGGTGGTCCATGTCCAT+GGG + Chr6:1656607-1656626 None:intergenic 50.0%
AAGGTCACGGGTTCAAGTCC+TGG - Chr6:1653558-1653577 MsG0680030396.01.T01:CDS 55.0%
ACTGAAGCTCCCGCATATGC+AGG + Chr6:1653655-1653674 None:intergenic 55.0%
ACTGGTGTTTGGGAGGCTGA+GGG + Chr6:1655631-1655650 None:intergenic 55.0%
CCAAACTGGTGCCCAACTGT+TGG + Chr6:1655530-1655549 None:intergenic 55.0%
CCAACAGTTGGGCACCAGTT+TGG - Chr6:1655527-1655546 MsG0680030396.01.T01:intron 55.0%
CTGAAGCTCCCGCATATGCA+GGG + Chr6:1653654-1653673 None:intergenic 55.0%
GATTTGGGTGGAGGAGTTCG+AGG - Chr6:1656012-1656031 MsG0680030396.01.T01:intron 55.0%
GCAGGGCCTTCTCCACATTT+CGG - Chr6:1655195-1655214 MsG0680030396.01.T01:intron 55.0%
TACTGGTGTTTGGGAGGCTG+AGG + Chr6:1655632-1655651 None:intergenic 55.0%
TCCATCTCCTACAGACAGCC+GGG + Chr6:1654698-1654717 None:intergenic 55.0%
! ACCACGCAAAGCCGCTTTGA+GGG + Chr6:1655446-1655465 None:intergenic 55.0%
AAAGCGGCTTTGCGTGGTCC+CGG - Chr6:1655448-1655467 MsG0680030396.01.T01:intron 60.0%
AGGGGTAACCTTGGCGCAAC+TGG - Chr6:1653512-1653531 MsG0680030396.01.T01:CDS 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG - Chr6:1653659-1653678 MsG0680030396.01.T01:intron 60.0%
CATATGCAGGGTCCGGGAAG+GGG + Chr6:1653642-1653661 None:intergenic 60.0%
CGTGAGACAGCCCATGGACA+TGG - Chr6:1656594-1656613 MsG0680030396.01.T01:intron 60.0%
CTTCCCGGACCCTGCATATG+CGG - Chr6:1653642-1653661 MsG0680030396.01.T01:CDS 60.0%
GCATATGCAGGGTCCGGGAA+GGG + Chr6:1653643-1653662 None:intergenic 60.0%
GGCAAGGGACCTGACTGAGA+TGG + Chr6:1655823-1655842 None:intergenic 60.0%
TCCCTCAAAGCGGCTTTGCG+TGG - Chr6:1655442-1655461 MsG0680030396.01.T01:intron 60.0%
TTCCCGGACCCTGCATATGC+GGG - Chr6:1653643-1653662 MsG0680030396.01.T01:CDS 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - Chr6:1653627-1653646 MsG0680030396.01.T01:CDS 60.0%
! GACCACGCAAAGCCGCTTTG+AGG + Chr6:1655447-1655466 None:intergenic 60.0%
CGCATATGCAGGGTCCGGGA+AGG + Chr6:1653644-1653663 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG + Chr6:1653629-1653648 None:intergenic 65.0%
CTCCCGCATATGCAGGGTCC+GGG + Chr6:1653648-1653667 None:intergenic 65.0%
GCCCGGCTGTCTGTAGGAGA+TGG - Chr6:1654694-1654713 MsG0680030396.01.T01:CDS 65.0%
GCTCCCGCATATGCAGGGTC+CGG + Chr6:1653649-1653668 None:intergenic 65.0%
TCAGGTGCCCGGCTGTCTGT+AGG - Chr6:1654688-1654707 MsG0680030396.01.T01:CDS 65.0%
TGCGGGAGCTTCAGTGCACC+GGG - Chr6:1653660-1653679 MsG0680030396.01.T01:intron 65.0%
GCGGCTTTGCGTGGTCCCGG+AGG - Chr6:1655451-1655470 MsG0680030396.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 1653509 1656629 1653509 ID=MsG0680030396.01;Name=MsG0680030396.01
Chr6 mRNA 1653509 1656629 1653509 ID=MsG0680030396.01.T01;Parent=MsG0680030396.01;Name=MsG0680030396.01.T01;_AED=0.52;_eAED=0.54;_QI=0|0|0|1|0.75|0.8|5|0|367
Chr6 exon 1656605 1656629 1656605 ID=MsG0680030396.01.T01:exon:2695;Parent=MsG0680030396.01.T01
Chr6 exon 1655367 1655446 1655367 ID=MsG0680030396.01.T01:exon:2694;Parent=MsG0680030396.01.T01
Chr6 exon 1654904 1655047 1654904 ID=MsG0680030396.01.T01:exon:2693;Parent=MsG0680030396.01.T01
Chr6 exon 1654088 1654783 1654088 ID=MsG0680030396.01.T01:exon:2692;Parent=MsG0680030396.01.T01
Chr6 exon 1653509 1653667 1653509 ID=MsG0680030396.01.T01:exon:2691;Parent=MsG0680030396.01.T01
Chr6 CDS 1656605 1656629 1656605 ID=MsG0680030396.01.T01:cds;Parent=MsG0680030396.01.T01
Chr6 CDS 1655367 1655446 1655367 ID=MsG0680030396.01.T01:cds;Parent=MsG0680030396.01.T01
Chr6 CDS 1654904 1655047 1654904 ID=MsG0680030396.01.T01:cds;Parent=MsG0680030396.01.T01
Chr6 CDS 1654088 1654783 1654088 ID=MsG0680030396.01.T01:cds;Parent=MsG0680030396.01.T01
Chr6 CDS 1653509 1653667 1653509 ID=MsG0680030396.01.T01:cds;Parent=MsG0680030396.01.T01
Gene Sequence

>MsG0680030396.01.T01

ATGAGGGGTAACCTTGGCGCAACTGGTGCCCGGCTGTCTGTAGGAGATGGAACACTTACAATAGTTACCAAGAATCAATCTGTAATGGATAAAACACTAGAAAAGGTTGTTTCTATAGTGGGCCGTGAAATTGAAGTTGGAGGTATCTACAAAGGTGTTGTCACCTCTATAAAAGAATATGGAGCTTTTGTTGAATTTAATGGTGGCAAGCAGGGCCTTCTCCACATTTCGGAGTTATCACATGAACCGGTTTCCAAGATTTCAGACATAGTCTCTATTGGCCAGCAGCTTTCTTTGATGTGCATTGGCCAAGATGTTCATGGTAACATTAAATTATCCCTCAAAGCGGCTTTGCGTGGTCCCGGAGGATCAGAGACTAATTATATAGCCGATGTATCTGCCACATCTGCAAAAGTAACTACCAACAGTTGGGCACCAGTTTGGGACACGTCTAGTATTACACAAGAACAAAATTCTGCTTCCGAGTTGCCTATAGAAAAAAATGAGGTGGGCGAAACAAATCCCTCAGCCTCCCAAACACCAGTAATTGTAATACGCAGTGCTGCAGAATGTGATGAGGAGGAGAAATCTATCAGCTTGGATCACAATCAAACTTCAAATGGTCCTCTCATTGATGATAGGGTAAAATTGGATAGTAAGTCGAAATCTCCATCCAAGTCAAAACCTCGTAAATCTCAATATGCAATTGATTCTCCATCTCAGTCAGGTCCCTTGCCAAAGAAGCCAAAACATTCAACGCAAAAAGAGTCGAAATCTGATACTCAAAGGGCAGAAGGGAACGAGAAGAAAGATAAAGACAATGAAACATTGACTGCAAAAGATTTGCCACTTGGAACAAAATTTAAAGCCAAAGTTTTTCAAATTCGTGCACATGGGTTAGTGTTAGATTTGGGTGGAGGAGTTCGAGGAATGTACCGTTTTGAGGGAGATGGGAAAACGGACTTGAAGGTAGGCGATGAGATGCAAGTAGTGCTCTCAAGCTTTTCTAGCAAGGGAATTCCGGTAGTGTTCCTGTGGATGATAAATAACTTAAGCTGTTGCTTCTTTCGTGAGACAGCCCATGGACATGGACCACCAAGATGA
Protein sequence

>MsG0680030396.01.T01

MRGNLGATGARLSVGDGTLTIVTKNQSVMDKTLEKVVSIVGREIEVGGIYKGVVTSIKEYGAFVEFNGGKQGLLHISELSHEPVSKISDIVSIGQQLSLMCIGQDVHGNIKLSLKAALRGPGGSETNYIADVSATSAKVTTNSWAPVWDTSSITQEQNSASELPIEKNEVGETNPSASQTPVIVIRSAAECDEEEKSISLDHNQTSNGPLIDDRVKLDSKSKSPSKSKPRKSQYAIDSPSQSGPLPKKPKHSTQKESKSDTQRAEGNEKKDKDNETLTAKDLPLGTKFKAKVFQIRAHGLVLDLGGGVRGMYRFEGDGKTDLKVGDEMQVVLSSFSSKGIPVVFLWMINNLSCCFFRETAHGHGPPR*