AlfalfaGEDB | MsG0680030565.01

M. sativa cultivar ZhongmuNo.1 / MsG0680030565.01

KEGG: -
GO: GO:0016747
PFAM: PF02458

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030565.01.T01	XP_013451090.1	81.846	325	32	2	1	300	138	460	0	530

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030565.01.T01	Q940Z5	33.923	339	174	12	1	298	139	468	1.13E-47	169

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030565.01.T01	A0A072UH54	81.846	325	32	2	1	300	138	460	0.0	530

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0580024443.01	MsG0680030565.01	0.822008	2.979919e-53	2.463679e-50
MsG0680030564.01	MsG0680030565.01	0.884227	2.296582e-71	1.503297e-67
MsG0680030565.01	MsG0780038948.01	0.829276	5.634267e-55	5.740291e-52

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030565.01.T01	MTR_6g015850	81.846	325	32	2	1	300	138	460	0.0	530
MsG0680030565.01.T01	MTR_6g015830	80.615	325	38	1	1	300	139	463	0.0	529
MsG0680030565.01.T01	MTR_6g015815	72.086	326	65	2	1	300	138	463	2.51e-166	470
MsG0680030565.01.T01	MTR_6g015315	51.515	330	125	7	1	300	131	455	1.12e-107	320
MsG0680030565.01.T01	MTR_6g015320	48.640	331	132	7	1	300	134	457	1.22e-96	292
MsG0680030565.01.T01	MTR_6g015310	48.201	278	118	7	1	275	79	333	3.69e-80	246
MsG0680030565.01.T01	MTR_7g014230	41.617	334	150	9	4	300	136	461	2.22e-77	243
MsG0680030565.01.T01	MTR_7g014250	40.597	335	150	10	4	299	134	458	1.01e-74	236
MsG0680030565.01.T01	MTR_7g014330	40.000	335	152	10	4	299	134	458	1.27e-74	236
MsG0680030565.01.T01	MTR_7g014360	41.456	316	154	11	4	299	84	388	2.26e-71	225

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030565.01.T01	AT3G29635	34.940	332	174	7	1	300	136	457	2.49e-55	186
MsG0680030565.01.T01	AT5G39090	33.333	327	177	8	1	298	134	448	8.14e-52	176
MsG0680030565.01.T01	AT5G39080	33.735	332	173	11	1	293	134	457	8.42e-51	174
MsG0680030565.01.T01	AT5G39050	33.923	339	174	12	1	298	139	468	1.16e-48	169
MsG0680030565.01.T01	AT3G29590	33.739	329	176	10	1	298	132	449	3.02e-48	167
MsG0680030565.01.T01	AT3G29670	32.110	327	184	7	1	299	135	451	1.45e-47	165
MsG0680030565.01.T01	AT5G61160	28.834	326	185	7	6	297	138	450	8.48e-42	150
MsG0680030565.01.T01	AT3G29680	31.250	320	170	9	14	298	144	448	4.25e-41	148
MsG0680030565.01.T01	AT1G03940	27.879	330	181	9	10	298	152	465	2.06e-28	114
MsG0680030565.01.T01	AT3G29636	35.149	202	90	7	111	300	23	195	1.04e-21	90.9

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Find 32 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAAAGCAGAAGAACCTA+AGG	0.532232	6:+5334505	MsG0680030565.01.T01:CDS
AACAATGGTGGAATTGAGAT+TGG	0.373662	6:+5334907	MsG0680030565.01.T01:CDS
AATATGTCTAGTGGAATGGA+AGG	0.600539	6:+5334751	MsG0680030565.01.T01:CDS
ACACATCATGCTGCTTTAGA+TGG	0.395750	6:+5334107	MsG0680030565.01.T01:CDS
ACTCTATTGCTGGATCACCA+AGG	0.676816	6:+5334782	MsG0680030565.01.T01:CDS
AGAATTAGAACATGTAATAT+GGG	0.428687	6:-5334162	None:intergenic
AGTGAAAGTAGGAATAACAA+TGG	0.608579	6:+5334892	MsG0680030565.01.T01:CDS
AGTTACATGTGCTTATGTTT+TGG	0.261109	6:+5334475	MsG0680030565.01.T01:CDS
ATACTATAAAACTCAAACCT+TGG	0.492343	6:-5334799	None:intergenic
ATCAATGATCTATCAAATAA+AGG	0.374744	6:-5334227	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTGGTTTCATATTTGGTA+TGG	+	Chr6:5334534-5334553	MsG0680030565.01.T01:intron	30.0%
AGTGAAAGTAGGAATAACAA+TGG	+	Chr6:5334892-5334911	MsG0680030565.01.T01:CDS	30.0%
ATGACTTCAACTGATAAATC+AGG	+	Chr6:5334856-5334875	MsG0680030565.01.T01:CDS	30.0%
CTTAAATGGAGCTGAAAATT+GGG	+	Chr6:5334723-5334742	MsG0680030565.01.T01:CDS	30.0%
GGTAAAATTTACTCTATTGC+TGG	+	Chr6:5334772-5334791	MsG0680030565.01.T01:CDS	30.0%
TCTTAAATGGAGCTGAAAAT+TGG	+	Chr6:5334722-5334741	MsG0680030565.01.T01:CDS	30.0%
TTCTAATATGTCTAGTGGAA+TGG	+	Chr6:5334747-5334766	MsG0680030565.01.T01:CDS	30.0%
AACAAAAGCAGAAGAACCTA+AGG	+	Chr6:5334505-5334524	MsG0680030565.01.T01:CDS	35.0%
AATATGTCTAGTGGAATGGA+AGG	+	Chr6:5334751-5334770	MsG0680030565.01.T01:CDS	35.0%
AGAACCTAAGGTTGAAAAAG+TGG	+	Chr6:5334517-5334536	MsG0680030565.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	5334023	5334999	5334023	ID=MsG0680030565.01;Name=MsG0680030565.01
Chr6	mRNA	5334023	5334999	5334023	ID=MsG0680030565.01.T01;Parent=MsG0680030565.01;Name=MsG0680030565.01.T01;_AED=0.21;_eAED=0.22;_QI=0\|0\|0\|1\|1\|1\|2\|0\|300
Chr6	exon	5334023	5334550	5334023	ID=MsG0680030565.01.T01:exon:4380;Parent=MsG0680030565.01.T01
Chr6	exon	5334625	5334999	5334625	ID=MsG0680030565.01.T01:exon:4381;Parent=MsG0680030565.01.T01
Chr6	CDS	5334023	5334550	5334023	ID=MsG0680030565.01.T01:cds;Parent=MsG0680030565.01.T01
Chr6	CDS	5334625	5334999	5334625	ID=MsG0680030565.01.T01:cds;Parent=MsG0680030565.01.T01

Gene Sequence

>MsG0680030565.01.T01

TTGAAAAATTCTCATGAAAAGGCTTCTTTACTTGCAATACAAGTCACTTTGTTTCCAAACAATGGTTTTTGCATTGGAATAACAACACATCATGCTGCTTTAGATGGAAGATCTGCAACTACTTTTATGAAATCATGGTCCCATATTACATGTTCTAATTCTAAACTTGATTCATCTTTTTTATCATTACCTGAAAATCTAACACCTTTATTTGATAGATCATTGATACAAGACCATAATAATGGAATTGGAATTAGTGAAGCATATGTTGTTGATTTGATGAAACAAGGTGGACCAAATAATAAAAGTCTCAAGATTATGAATTTTTCTGGTCCAATGAAACATGATCTAGTTAAAAGTTTGTATGAATTGACACCTTCAAATATTCAAAAGCTTAAAGAACATGCAAAAAATGAGATGAAAATGAATGCTATAAATTTGTCAACTTTTTCAGTTACATGTGCTTATGTTTTGGCATGTTTAACAAAAGCAGAAGAACCTAAGGTTGAAAAAGTGGTTTCATATTTGATTGTGTTTGAGACAAAGAACTTAGTTGGAAAAAATGGATTTTTAAATGCTTTAGAAGGGATAAATAAAGCTTTGAATAGTGTGAAAGATTTTGGAGTCTTAAATGGAGCTGAAAATTGGGTTTCTAATATGTCTAGTGGAATGGAAGGTAAAATTTACTCTATTGCTGGATCACCAAGGTTTGAGTTTTATAGTATTGATTTTGGATTTGGAAAGCCAAAAAAAGTTGATATGACTTCAACTGATAAATCAGGAGCATTTTCTATTAGTGAAAGTAGGAATAACAATGGTGGAATTGAGATTGGTTTGGCTTTAAGTAAGGAAGAGATGGAAGCTTTTTCTACCCTTTTTATTCAAGGACTTGAATCCATTTAA

Protein sequence

>MsG0680030565.01.T01

LKNSHEKASLLAIQVTLFPNNGFCIGITTHHAALDGRSATTFMKSWSHITCSNSKLDSSFLSLPENLTPLFDRSLIQDHNNGIGISEAYVVDLMKQGGPNNKSLKIMNFSGPMKHDLVKSLYELTPSNIQKLKEHAKNEMKMNAINLSTFSVTCAYVLACLTKAEEPKVEKVVSYLIVFETKNLVGKNGFLNALEGINKALNSVKDFGVLNGAENWVSNMSSGMEGKIYSIAGSPRFEFYSIDFGFGKPKKVDMTSTDKSGAFSISESRNNNGGIEIGLALSKEEMEAFSTLFIQGLESI*