Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031067.01.T01 | MCH80942.1 | 77.143 | 175 | 40 | 0 | 1 | 175 | 1 | 175 | 3.78E-91 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031067.01.T01 | A0A392M0U0 | 77.143 | 175 | 40 | 0 | 1 | 175 | 1 | 175 | 1.81e-91 | 273 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480020748.01 | MsG0680031067.01 | 0.801028 | 1.092462e-48 | 5.163859e-46 |
| MsG0280007331.01 | MsG0680031067.01 | 0.827282 | 1.705395e-54 | 1.638988e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031067.01.T01 | MTR_6g017215 | 72.571 | 175 | 48 | 0 | 1 | 175 | 15 | 189 | 5.18e-83 | 245 |
| MsG0680031067.01.T01 | MTR_5g028130 | 63.483 | 178 | 65 | 0 | 2 | 179 | 157 | 334 | 5.42e-72 | 221 |
| MsG0680031067.01.T01 | MTR_5g028190 | 63.218 | 174 | 64 | 0 | 3 | 176 | 354 | 527 | 1.54e-69 | 220 |
| MsG0680031067.01.T01 | MTR_5g028450 | 61.957 | 184 | 66 | 2 | 2 | 181 | 112 | 295 | 6.20e-65 | 202 |
| MsG0680031067.01.T01 | MTR_5g028110 | 53.107 | 177 | 65 | 1 | 3 | 179 | 13 | 171 | 6.12e-56 | 174 |
| MsG0680031067.01.T01 | MTR_5g028690 | 48.352 | 182 | 89 | 2 | 3 | 179 | 52 | 233 | 3.15e-50 | 162 |
| MsG0680031067.01.T01 | MTR_2g438740 | 48.235 | 170 | 84 | 3 | 4 | 169 | 389 | 558 | 3.29e-45 | 157 |
| MsG0680031067.01.T01 | MTR_5g028930 | 46.448 | 183 | 92 | 3 | 1 | 178 | 432 | 613 | 2.16e-44 | 155 |
| MsG0680031067.01.T01 | MTR_3g055310 | 42.945 | 163 | 92 | 1 | 1 | 162 | 165 | 327 | 1.22e-38 | 135 |
| MsG0680031067.01.T01 | MTR_3g055310 | 42.945 | 163 | 92 | 1 | 1 | 162 | 228 | 390 | 3.36e-38 | 135 |
| MsG0680031067.01.T01 | MTR_3g055310 | 42.945 | 163 | 92 | 1 | 1 | 162 | 280 | 442 | 1.11e-37 | 135 |
| MsG0680031067.01.T01 | MTR_3g055310 | 42.945 | 163 | 92 | 1 | 1 | 162 | 333 | 495 | 1.20e-37 | 135 |
| MsG0680031067.01.T01 | MTR_3g055310 | 42.945 | 163 | 92 | 1 | 1 | 162 | 306 | 468 | 1.35e-37 | 135 |
| MsG0680031067.01.T01 | MTR_3g055310 | 42.945 | 163 | 92 | 1 | 1 | 162 | 308 | 470 | 1.42e-37 | 135 |
| MsG0680031067.01.T01 | MTR_5g091220 | 42.938 | 177 | 100 | 1 | 1 | 176 | 421 | 597 | 7.21e-36 | 132 |
| MsG0680031067.01.T01 | MTR_5g028900 | 45.517 | 145 | 67 | 3 | 1 | 145 | 11 | 143 | 4.83e-34 | 118 |
| MsG0680031067.01.T01 | MTR_5g028840 | 39.247 | 186 | 98 | 5 | 4 | 179 | 14 | 194 | 1.21e-33 | 119 |
| MsG0680031067.01.T01 | MTR_2g438400 | 37.430 | 179 | 110 | 2 | 4 | 181 | 368 | 545 | 2.82e-32 | 121 |
| MsG0680031067.01.T01 | MTR_2g438440 | 39.106 | 179 | 107 | 2 | 4 | 181 | 509 | 686 | 4.64e-32 | 121 |
| MsG0680031067.01.T01 | MTR_2g438720 | 39.560 | 182 | 102 | 5 | 6 | 182 | 517 | 695 | 1.53e-31 | 120 |
| MsG0680031067.01.T01 | MTR_2g438700 | 39.548 | 177 | 101 | 4 | 6 | 179 | 515 | 688 | 6.67e-31 | 118 |
| MsG0680031067.01.T01 | MTR_3g455790 | 37.805 | 164 | 101 | 1 | 1 | 163 | 416 | 579 | 2.72e-30 | 116 |
| MsG0680031067.01.T01 | MTR_2g438640 | 38.182 | 165 | 92 | 2 | 6 | 170 | 490 | 644 | 7.69e-29 | 112 |
| MsG0680031067.01.T01 | MTR_2g438640 | 38.182 | 165 | 92 | 2 | 6 | 170 | 413 | 567 | 7.74e-29 | 112 |
| MsG0680031067.01.T01 | MTR_2g438640 | 38.182 | 165 | 92 | 2 | 6 | 170 | 516 | 670 | 8.65e-29 | 112 |
| MsG0680031067.01.T01 | MTR_2g438760 | 39.552 | 134 | 81 | 0 | 4 | 137 | 383 | 516 | 1.15e-27 | 108 |
| MsG0680031067.01.T01 | MTR_3g452020 | 33.951 | 162 | 106 | 1 | 2 | 162 | 242 | 403 | 1.74e-27 | 107 |
| MsG0680031067.01.T01 | MTR_2g020510 | 36.047 | 172 | 107 | 2 | 2 | 172 | 500 | 669 | 4.10e-27 | 107 |
| MsG0680031067.01.T01 | MTR_3g452020 | 33.951 | 162 | 106 | 1 | 2 | 162 | 418 | 579 | 5.98e-27 | 106 |
| MsG0680031067.01.T01 | MTR_2g020470 | 32.558 | 172 | 113 | 2 | 2 | 172 | 482 | 651 | 3.10e-25 | 102 |
| MsG0680031067.01.T01 | MTR_3g050500 | 35.971 | 139 | 88 | 1 | 6 | 144 | 547 | 684 | 9.80e-23 | 94.7 |
| MsG0680031067.01.T01 | MTR_2g020450 | 35.593 | 177 | 100 | 3 | 2 | 172 | 503 | 671 | 3.20e-22 | 93.2 |
| MsG0680031067.01.T01 | MTR_3g058570 | 39.098 | 133 | 81 | 0 | 7 | 139 | 204 | 336 | 1.29e-21 | 90.9 |
| MsG0680031067.01.T01 | MTR_5g085360 | 32.278 | 158 | 104 | 2 | 7 | 163 | 557 | 712 | 3.29e-20 | 87.4 |
| MsG0680031067.01.T01 | MTR_5g085350 | 37.107 | 159 | 96 | 3 | 6 | 162 | 556 | 712 | 5.44e-19 | 84.0 |
| MsG0680031067.01.T01 | MTR_5g085350 | 37.107 | 159 | 96 | 3 | 6 | 162 | 543 | 699 | 6.32e-19 | 84.0 |
| MsG0680031067.01.T01 | MTR_2g017930 | 36.095 | 169 | 103 | 3 | 6 | 172 | 469 | 634 | 4.59e-18 | 81.3 |
| MsG0680031067.01.T01 | MTR_5g085340 | 34.395 | 157 | 101 | 2 | 7 | 162 | 565 | 720 | 8.75e-18 | 80.5 |
| MsG0680031067.01.T01 | MTR_3g051060 | 28.415 | 183 | 127 | 3 | 2 | 181 | 548 | 729 | 3.61e-16 | 75.9 |
| MsG0680031067.01.T01 | MTR_2g438580 | 40.964 | 83 | 48 | 1 | 6 | 88 | 2 | 83 | 3.97e-15 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031067.01.T01 | AT3G18670 | 44.578 | 166 | 86 | 4 | 13 | 173 | 416 | 580 | 1.20e-38 | 139 |
| MsG0680031067.01.T01 | AT3G18670 | 44.578 | 166 | 86 | 4 | 13 | 173 | 471 | 635 | 1.77e-38 | 139 |
| MsG0680031067.01.T01 | AT3G54065 | 43.919 | 148 | 77 | 1 | 2 | 143 | 32 | 179 | 1.03e-37 | 130 |
| MsG0680031067.01.T01 | AT5G04700 | 43.860 | 171 | 92 | 3 | 3 | 171 | 476 | 644 | 2.05e-36 | 134 |
| MsG0680031067.01.T01 | AT5G04690 | 40.828 | 169 | 96 | 3 | 4 | 171 | 455 | 620 | 5.17e-35 | 129 |
| MsG0680031067.01.T01 | AT5G04690 | 40.828 | 169 | 96 | 3 | 4 | 171 | 469 | 634 | 7.10e-35 | 129 |
| MsG0680031067.01.T01 | AT5G35810 | 43.038 | 158 | 84 | 2 | 1 | 152 | 146 | 303 | 2.17e-34 | 124 |
| MsG0680031067.01.T01 | AT5G04690 | 38.333 | 180 | 109 | 2 | 3 | 181 | 434 | 612 | 1.51e-33 | 125 |
| MsG0680031067.01.T01 | AT5G04680 | 38.547 | 179 | 98 | 3 | 3 | 171 | 340 | 516 | 1.82e-32 | 122 |
| MsG0680031067.01.T01 | AT5G04680 | 38.547 | 179 | 98 | 3 | 3 | 171 | 449 | 625 | 3.31e-32 | 122 |
| MsG0680031067.01.T01 | AT5G04680 | 38.547 | 179 | 98 | 3 | 3 | 171 | 463 | 639 | 3.42e-32 | 122 |
| MsG0680031067.01.T01 | AT5G04680 | 38.547 | 179 | 98 | 3 | 3 | 171 | 492 | 668 | 3.90e-32 | 121 |
| MsG0680031067.01.T01 | AT5G04730 | 37.356 | 174 | 109 | 0 | 6 | 179 | 414 | 587 | 1.16e-30 | 117 |
Find 34 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTGAGATTTGCCATTCT+TGG | 0.218107 | 6:+13760915 | MsG0680031067.01.T01:CDS |
| GCGGCAGCTTTCACTGTTCC+AGG | 0.293026 | 6:+13760551 | MsG0680031067.01.T01:CDS |
| TTATCATTGAAGGTCGAAAA+TGG | 0.301551 | 6:+13760468 | MsG0680031067.01.T01:CDS |
| TACCGCTGGATGATAATAAC+AGG | 0.383301 | 6:+13760836 | MsG0680031067.01.T01:CDS |
| CATTGTGCCTATGATGTATA+AGG | 0.386464 | 6:+13760385 | None:intergenic |
| TTTGCTTGATCGTTTCCTCC+TGG | 0.419859 | 6:-13760569 | None:intergenic |
| ATGCTGAGGAAGACTTCCTT+TGG | 0.426019 | 6:+13760711 | MsG0680031067.01.T01:CDS |
| CATCATTACTATCATGTTTG+CGG | 0.429986 | 6:+13760532 | MsG0680031067.01.T01:CDS |
| GGCAAGTTCTTTCACAATTG+TGG | 0.437752 | 6:+13760502 | MsG0680031067.01.T01:CDS |
| GCAAGTTCTTTCACAATTGT+GGG | 0.442272 | 6:+13760503 | MsG0680031067.01.T01:CDS |
| CTTGTCTGTGCTATCCATGA+TGG | 0.445968 | 6:+13760775 | MsG0680031067.01.T01:CDS |
| TGGACTTGCTCTCATGCTGA+AGG | 0.447431 | 6:+13760811 | MsG0680031067.01.T01:CDS |
| AAGGGCAAATAGTAACTTCA+AGG | 0.474040 | 6:-13760741 | None:intergenic |
| AGACAAGAATATTGTGAAAA+GGG | 0.474981 | 6:-13760759 | None:intergenic |
| CAGACAAGAATATTGTGAAA+AGG | 0.476674 | 6:-13760760 | None:intergenic |
| ACTACATACTCCACCAAGAA+TGG | 0.487863 | 6:-13760928 | None:intergenic |
| TATCATTGAAGGTCGAAAAT+GGG | 0.498999 | 6:+13760469 | MsG0680031067.01.T01:CDS |
| GGACTTGCTCTCATGCTGAA+GGG | 0.502536 | 6:+13760812 | MsG0680031067.01.T01:CDS |
| GTGAGATTTGCCATTCTTGG+TGG | 0.509119 | 6:+13760918 | MsG0680031067.01.T01:CDS |
| CATGGGCAGCTTATCATTGA+AGG | 0.512726 | 6:+13760458 | MsG0680031067.01.T01:CDS |
| GTGAACAGTAACACTATGAT+TGG | 0.513432 | 6:-13760878 | None:intergenic |
| GACTTGCTCTCATGCTGAAG+GGG | 0.522570 | 6:+13760813 | MsG0680031067.01.T01:CDS |
| TCATGCTGAAGGGGTACCGC+TGG | 0.539166 | 6:+13760822 | MsG0680031067.01.T01:CDS |
| GGAGGAAACGATCAAGCAAA+GGG | 0.554355 | 6:+13760572 | MsG0680031067.01.T01:CDS |
| TCGAAAATGGGCAAAAGAGA+CGG | 0.554544 | 6:+13760481 | MsG0680031067.01.T01:CDS |
| AGGAGGAAACGATCAAGCAA+AGG | 0.598759 | 6:+13760571 | MsG0680031067.01.T01:CDS |
| ACTTCAAGGGCAATCTCCAA+AGG | 0.612141 | 6:-13760727 | None:intergenic |
| CTGGATGATAATAACAGGGA+TGG | 0.613254 | 6:+13760841 | MsG0680031067.01.T01:CDS |
| TCTTACATCGCGTTATGCTG+AGG | 0.613592 | 6:+13760697 | MsG0680031067.01.T01:CDS |
| AGGGCAAATAGTAACTTCAA+GGG | 0.622977 | 6:-13760740 | None:intergenic |
| CAGCATGAGAGCAAGTCCAA+AGG | 0.641102 | 6:-13760807 | None:intergenic |
| GCAGCTTTCACTGTTCCAGG+AGG | 0.688478 | 6:+13760554 | MsG0680031067.01.T01:CDS |
| TCCCTGTTATTATCATCCAG+CGG | 0.701926 | 6:-13760838 | None:intergenic |
| ACCGCTGGATGATAATAACA+GGG | 0.711385 | 6:+13760837 | MsG0680031067.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTATTTACCAAAAATCAT+GGG | + | Chr6:13760441-13760460 | MsG0680031067.01.T01:CDS | 20.0% |
| ! | AGACAAGAATATTGTGAAAA+GGG | - | Chr6:13760762-13760781 | None:intergenic | 25.0% |
| !! | GAAGTATTTACCAAAAATCA+TGG | + | Chr6:13760440-13760459 | MsG0680031067.01.T01:CDS | 25.0% |
| CAGACAAGAATATTGTGAAA+AGG | - | Chr6:13760763-13760782 | None:intergenic | 30.0% | |
| CATCATTACTATCATGTTTG+CGG | + | Chr6:13760532-13760551 | MsG0680031067.01.T01:CDS | 30.0% | |
| TATCATTGAAGGTCGAAAAT+GGG | + | Chr6:13760469-13760488 | MsG0680031067.01.T01:CDS | 30.0% | |
| TTATCATTGAAGGTCGAAAA+TGG | + | Chr6:13760468-13760487 | MsG0680031067.01.T01:CDS | 30.0% | |
| ! | GCATCTTTTCCTAAAAAGAA+AGG | - | Chr6:13760602-13760621 | None:intergenic | 30.0% |
| !! | CATCAGTTTTGACGTTTATT+GGG | + | Chr6:13760672-13760691 | MsG0680031067.01.T01:CDS | 30.0% |
| !! | TCATCAGTTTTGACGTTTAT+TGG | + | Chr6:13760671-13760690 | MsG0680031067.01.T01:CDS | 30.0% |
| !! | TTTTTGGTAAATACTTCACG+AGG | - | Chr6:13760437-13760456 | None:intergenic | 30.0% |
| AAAGGAAAAAGCAACCATCA+TGG | - | Chr6:13760792-13760811 | None:intergenic | 35.0% | |
| AAGGGCAAATAGTAACTTCA+AGG | - | Chr6:13760744-13760763 | None:intergenic | 35.0% | |
| AGGGCAAATAGTAACTTCAA+GGG | - | Chr6:13760743-13760762 | None:intergenic | 35.0% | |
| GCAAGTTCTTTCACAATTGT+GGG | + | Chr6:13760503-13760522 | MsG0680031067.01.T01:CDS | 35.0% | |
| GTGAACAGTAACACTATGAT+TGG | - | Chr6:13760881-13760900 | None:intergenic | 35.0% | |
| TTTGTGAGATTTGCCATTCT+TGG | + | Chr6:13760915-13760934 | MsG0680031067.01.T01:CDS | 35.0% | |
| ! | AAGGGAACACCTTTCTTTTT+AGG | + | Chr6:13760590-13760609 | MsG0680031067.01.T01:CDS | 35.0% |
| !!! | ATGATGGTTGCTTTTTCCTT+TGG | + | Chr6:13760791-13760810 | MsG0680031067.01.T01:CDS | 35.0% |
| AAGGAAGCCAAAAATGCAGA+CGG | + | Chr6:13760404-13760423 | MsG0680031067.01.T01:CDS | 40.0% | |
| ACCGCTGGATGATAATAACA+GGG | + | Chr6:13760837-13760856 | MsG0680031067.01.T01:CDS | 40.0% | |
| CTGGATGATAATAACAGGGA+TGG | + | Chr6:13760841-13760860 | MsG0680031067.01.T01:CDS | 40.0% | |
| GGCAAGTTCTTTCACAATTG+TGG | + | Chr6:13760502-13760521 | MsG0680031067.01.T01:CDS | 40.0% | |
| TACCGCTGGATGATAATAAC+AGG | + | Chr6:13760836-13760855 | MsG0680031067.01.T01:CDS | 40.0% | |
| TCCCTGTTATTATCATCCAG+CGG | - | Chr6:13760841-13760860 | None:intergenic | 40.0% | |
| TCGAAAATGGGCAAAAGAGA+CGG | + | Chr6:13760481-13760500 | MsG0680031067.01.T01:CDS | 40.0% | |
| ! | CTTAAGACCGTCTGCATTTT+TGG | - | Chr6:13760414-13760433 | None:intergenic | 40.0% |
| ! | GATAAGCTGCCCATGATTTT+TGG | - | Chr6:13760453-13760472 | None:intergenic | 40.0% |
| ACTTCAAGGGCAATCTCCAA+AGG | - | Chr6:13760730-13760749 | None:intergenic | 45.0% | |
| AGGAGGAAACGATCAAGCAA+AGG | + | Chr6:13760571-13760590 | MsG0680031067.01.T01:CDS | 45.0% | |
| ATGCTGAGGAAGACTTCCTT+TGG | + | Chr6:13760711-13760730 | MsG0680031067.01.T01:CDS | 45.0% | |
| CATGGGCAGCTTATCATTGA+AGG | + | Chr6:13760458-13760477 | MsG0680031067.01.T01:CDS | 45.0% | |
| CTTGTCTGTGCTATCCATGA+TGG | + | Chr6:13760775-13760794 | MsG0680031067.01.T01:CDS | 45.0% | |
| GGAGGAAACGATCAAGCAAA+GGG | + | Chr6:13760572-13760591 | MsG0680031067.01.T01:CDS | 45.0% | |
| GTGAGATTTGCCATTCTTGG+TGG | + | Chr6:13760918-13760937 | MsG0680031067.01.T01:CDS | 45.0% | |
| TCTTACATCGCGTTATGCTG+AGG | + | Chr6:13760697-13760716 | MsG0680031067.01.T01:CDS | 45.0% | |
| TTTGCTTGATCGTTTCCTCC+TGG | - | Chr6:13760572-13760591 | None:intergenic | 45.0% | |
| CAGCATGAGAGCAAGTCCAA+AGG | - | Chr6:13760810-13760829 | None:intergenic | 50.0% | |
| ! | GACTTGCTCTCATGCTGAAG+GGG | + | Chr6:13760813-13760832 | MsG0680031067.01.T01:CDS | 50.0% |
| ! | GGACTTGCTCTCATGCTGAA+GGG | + | Chr6:13760812-13760831 | MsG0680031067.01.T01:CDS | 50.0% |
| ! | TGGACTTGCTCTCATGCTGA+AGG | + | Chr6:13760811-13760830 | MsG0680031067.01.T01:CDS | 50.0% |
| GCAGCTTTCACTGTTCCAGG+AGG | + | Chr6:13760554-13760573 | MsG0680031067.01.T01:CDS | 55.0% | |
| GCGGCAGCTTTCACTGTTCC+AGG | + | Chr6:13760551-13760570 | MsG0680031067.01.T01:CDS | 60.0% | |
| !! | TCATGCTGAAGGGGTACCGC+TGG | + | Chr6:13760822-13760841 | MsG0680031067.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 13760398 | 13760949 | 13760398 | ID=MsG0680031067.01;Name=MsG0680031067.01 |
| Chr6 | mRNA | 13760398 | 13760949 | 13760398 | ID=MsG0680031067.01.T01;Parent=MsG0680031067.01;Name=MsG0680031067.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|183 |
| Chr6 | exon | 13760398 | 13760949 | 13760398 | ID=MsG0680031067.01.T01:exon:9585;Parent=MsG0680031067.01.T01 |
| Chr6 | CDS | 13760398 | 13760949 | 13760398 | ID=MsG0680031067.01.T01:cds;Parent=MsG0680031067.01.T01 |
| Gene Sequence |
| Protein sequence |
