AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031717.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031717.01.T01 MTR_6g033070 97.770 538 12 0 1 538 1 538 0.0 1079
MsG0680031717.01.T01 MTR_1g054630 35.266 414 252 5 134 534 148 558 8.45e-79 259
MsG0680031717.01.T01 MTR_2g100230 34.644 407 251 7 116 516 73 470 1.57e-76 250
MsG0680031717.01.T01 MTR_3g083690 34.783 414 252 6 132 530 137 547 7.15e-76 251
MsG0680031717.01.T01 MTR_4g114120 35.377 424 252 6 134 538 134 554 9.29e-73 244
MsG0680031717.01.T01 MTR_6g005550 33.941 439 271 9 106 538 130 555 6.85e-72 243
MsG0680031717.01.T01 MTR_3g467550 34.949 392 240 6 115 494 89 477 2.93e-71 238
MsG0680031717.01.T01 MTR_4g134170 32.000 425 256 8 121 520 267 683 3.93e-69 236
MsG0680031717.01.T01 MTR_6g005320 31.294 425 257 8 121 520 229 643 1.19e-68 233
MsG0680031717.01.T01 MTR_3g467550 35.475 358 219 5 146 494 92 446 1.13e-67 228
MsG0680031717.01.T01 MTR_4g105100 31.818 418 271 8 118 531 179 586 6.13e-67 228
MsG0680031717.01.T01 MTR_2g032630 33.097 423 268 7 103 516 453 869 9.60e-66 231
MsG0680031717.01.T01 MTR_1g095810 30.876 434 277 9 120 537 124 550 6.94e-63 216
MsG0680031717.01.T01 MTR_3g467550 33.846 390 238 7 92 464 61 447 2.50e-62 211
MsG0680031717.01.T01 MTR_1g026830 30.394 431 277 9 119 533 125 548 2.74e-61 212
MsG0680031717.01.T01 MTR_7g109720 30.549 419 274 8 109 518 199 609 2.11e-58 207
MsG0680031717.01.T01 MTR_5g027840 33.249 394 253 8 137 525 101 489 2.46e-56 197
MsG0680031717.01.T01 MTR_2g007000 30.673 401 269 6 121 516 484 880 1.44e-55 202
MsG0680031717.01.T01 MTR_7g100820 29.902 408 267 8 121 520 110 506 8.37e-53 189
MsG0680031717.01.T01 MTR_4g105100 32.362 343 223 7 123 461 184 521 2.59e-52 186
MsG0680031717.01.T01 MTR_7g056830 32.303 356 227 5 140 491 10 355 3.90e-51 181
MsG0680031717.01.T01 MTR_6g082080 31.421 366 237 5 140 501 10 365 1.47e-50 179
MsG0680031717.01.T01 MTR_3g049990 30.579 363 242 6 140 498 152 508 1.74e-50 185
MsG0680031717.01.T01 MTR_2g041140 30.790 367 237 8 140 498 141 498 6.76e-49 181
MsG0680031717.01.T01 MTR_4g125970 32.143 364 235 6 140 501 41 394 8.10e-49 174
MsG0680031717.01.T01 MTR_4g125970 32.143 364 235 6 140 501 58 411 1.23e-48 174
MsG0680031717.01.T01 MTR_2g039960 32.055 365 234 7 140 501 41 394 1.44e-48 174
MsG0680031717.01.T01 MTR_2g099620 31.978 369 229 7 140 501 25 378 3.74e-48 172
MsG0680031717.01.T01 MTR_2g099620 31.978 369 229 7 140 501 41 394 4.82e-48 172
MsG0680031717.01.T01 MTR_8g018400 31.593 364 237 6 140 501 41 394 5.86e-48 172
MsG0680031717.01.T01 MTR_7g076800 31.198 359 229 6 141 495 142 486 2.11e-47 172
MsG0680031717.01.T01 MTR_7g076830 31.198 359 229 6 141 495 144 488 2.32e-47 172
MsG0680031717.01.T01 MTR_7g076820 31.198 359 229 6 141 495 151 495 6.40e-47 171
MsG0680031717.01.T01 MTR_2g438110 28.610 374 235 7 140 501 34 387 9.77e-47 168
MsG0680031717.01.T01 MTR_5g090120 30.495 364 241 9 139 497 80 436 1.76e-46 171
MsG0680031717.01.T01 MTR_2g066050 30.233 387 250 7 140 521 53 424 4.39e-46 169
MsG0680031717.01.T01 MTR_4g079320 28.117 377 263 4 141 515 20 390 3.06e-45 170
MsG0680031717.01.T01 MTR_3g449250 30.811 370 242 7 132 496 82 442 7.47e-45 166
MsG0680031717.01.T01 MTR_3g449250 30.811 370 242 7 132 496 82 442 8.63e-45 167
MsG0680031717.01.T01 MTR_2g055850 31.267 371 230 12 132 490 85 442 6.34e-40 152
MsG0680031717.01.T01 MTR_4g059460 27.778 450 249 11 140 517 44 489 1.43e-39 152
MsG0680031717.01.T01 MTR_1g012420 30.645 372 231 10 131 491 38 393 1.79e-39 149
MsG0680031717.01.T01 MTR_3g107420 30.025 393 241 11 110 491 24 393 4.39e-39 148
MsG0680031717.01.T01 MTR_2g043360 27.529 425 231 10 140 490 41 462 1.32e-38 149
MsG0680031717.01.T01 MTR_4g010565 31.016 374 227 10 131 491 115 470 1.45e-38 148
MsG0680031717.01.T01 MTR_6g039840 27.344 384 261 9 138 506 108 488 2.58e-37 146
MsG0680031717.01.T01 MTR_3g071620 28.686 373 228 10 146 490 22 384 3.36e-37 145
MsG0680031717.01.T01 MTR_4g112340 31.988 347 210 15 158 490 115 449 5.44e-37 144
MsG0680031717.01.T01 MTR_8g027775 29.834 362 241 10 141 490 103 463 2.29e-36 142
MsG0680031717.01.T01 MTR_7g057250 25.986 431 247 9 140 501 163 590 2.00e-35 141
MsG0680031717.01.T01 MTR_6g056080 27.988 343 232 6 164 497 127 463 1.33e-34 139
MsG0680031717.01.T01 MTR_8g027770 30.458 371 227 13 141 490 202 562 1.55e-34 138
MsG0680031717.01.T01 MTR_8g027780 29.867 375 247 11 131 490 94 467 5.13e-34 136
MsG0680031717.01.T01 MTR_1g102410 25.199 377 251 6 146 497 50 420 1.91e-33 135
MsG0680031717.01.T01 MTR_0915s0010 30.612 294 186 6 154 443 3 282 2.67e-33 128
MsG0680031717.01.T01 MTR_3g118120 24.868 378 255 9 140 490 132 507 9.52e-31 126
MsG0680031717.01.T01 MTR_5g092560 26.567 335 233 9 163 490 99 427 2.15e-30 126
MsG0680031717.01.T01 MTR_1g083130 25.917 409 250 12 140 501 26 428 9.43e-29 120
MsG0680031717.01.T01 MTR_8g097000 26.764 411 272 13 117 504 259 663 6.92e-28 119
MsG0680031717.01.T01 MTR_4g068990 26.269 335 233 9 163 490 99 426 9.87e-28 118
MsG0680031717.01.T01 MTR_1g061970 27.225 382 235 12 140 490 80 449 1.34e-27 116
MsG0680031717.01.T01 MTR_8g027780 29.942 344 225 11 131 459 94 436 2.63e-26 112
MsG0680031717.01.T01 MTR_4g459350 32.407 216 123 5 140 333 41 255 1.58e-25 106
MsG0680031717.01.T01 MTR_6g039840 26.384 307 210 8 213 506 9 312 4.50e-25 108
MsG0680031717.01.T01 MTR_4g049910 26.702 382 235 10 140 497 55 415 4.64e-24 105
MsG0680031717.01.T01 MTR_4g077370 34.975 203 121 5 139 337 249 444 3.27e-23 102
MsG0680031717.01.T01 MTR_4g077370 34.419 215 105 6 139 344 1 188 1.85e-21 97.4
MsG0680031717.01.T01 MTR_4g112320 28.727 275 167 12 228 490 10 267 6.73e-23 101
MsG0680031717.01.T01 MTR_1g046570 26.343 391 234 12 155 506 6 381 7.35e-23 101
MsG0680031717.01.T01 MTR_4g131750 25.341 367 231 10 145 479 87 442 3.39e-22 100
MsG0680031717.01.T01 MTR_8g032230 35.669 157 94 3 138 293 8 158 4.25e-22 94.0
MsG0680031717.01.T01 MTR_5g069330 24.384 365 256 9 134 491 1 352 2.06e-20 94.0
MsG0680031717.01.T01 MTR_5g077690 23.608 449 267 13 145 523 34 476 2.41e-20 94.7
MsG0680031717.01.T01 MTR_5g069330 24.513 359 251 9 140 491 3 348 2.92e-20 93.6
MsG0680031717.01.T01 MTR_8g018370 25.816 337 213 9 169 501 2 305 2.04e-19 89.7
MsG0680031717.01.T01 MTR_2g043360 22.630 327 181 6 235 490 19 344 6.90e-19 90.1
MsG0680031717.01.T01 MTR_2g063080 27.160 243 150 6 131 346 98 340 2.30e-18 88.6
MsG0680031717.01.T01 MTR_3g107420 28.862 246 143 9 110 344 24 248 4.48e-17 82.8
MsG0680031717.01.T01 MTR_8g042690 23.034 356 219 12 163 490 41 369 4.37e-16 81.3
MsG0680031717.01.T01 MTR_5g092560 25.822 213 149 6 284 490 7 216 2.64e-14 75.9
MsG0680031717.01.T01 MTR_2g006440 25.753 365 214 14 151 489 398 731 5.54e-14 75.5
MsG0680031717.01.T01 MTR_4g068990 25.822 213 148 7 284 490 7 215 7.19e-14 74.7
MsG0680031717.01.T01 MTR_2g042440 24.658 365 224 14 158 498 39 376 3.38e-12 69.7
MsG0680031717.01.T01 MTR_2g042440 24.658 365 224 14 158 498 39 376 3.69e-12 69.3
MsG0680031717.01.T01 MTR_3g063040 23.167 341 218 11 158 481 13 326 1.25e-11 67.8
MsG0680031717.01.T01 MTR_8g062720 27.962 211 125 7 139 331 90 291 2.30e-11 66.6
MsG0680031717.01.T01 MTR_8g080160 23.770 366 226 12 149 493 266 599 3.13e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031717.01.T01 AT3G02065 65.927 496 146 4 27 518 14 490 0.0 679
MsG0680031717.01.T01 AT3G02065 65.927 496 146 4 27 518 14 490 0.0 679
MsG0680031717.01.T01 AT3G02065 71.105 353 101 1 167 518 1 353 0.0 531
MsG0680031717.01.T01 AT1G55150 33.170 407 257 7 117 516 76 474 4.25e-76 249
MsG0680031717.01.T01 AT1G55150 33.170 407 257 7 117 516 101 499 3.66e-75 247
MsG0680031717.01.T01 AT5G14610 33.487 433 270 9 111 537 132 552 5.25e-73 244
MsG0680031717.01.T01 AT5G14610 33.487 433 270 9 111 537 132 552 5.35e-73 244
MsG0680031717.01.T01 AT2G42520 34.293 417 245 7 134 530 154 561 5.55e-73 244
MsG0680031717.01.T01 AT2G42520 34.293 417 245 7 134 530 154 561 5.55e-73 244
MsG0680031717.01.T01 AT2G42520 34.293 417 245 7 134 530 154 561 5.55e-73 244
MsG0680031717.01.T01 AT3G58570 34.053 417 246 7 134 530 141 548 8.03e-73 244
MsG0680031717.01.T01 AT5G14610 33.487 433 270 9 111 537 140 560 1.06e-72 244
MsG0680031717.01.T01 AT5G63120 32.080 399 264 6 121 516 146 540 1.23e-72 242
MsG0680031717.01.T01 AT3G01540 33.176 425 265 9 116 534 134 545 5.01e-72 241
MsG0680031717.01.T01 AT3G01540 33.176 425 265 9 116 534 134 545 5.12e-72 241
MsG0680031717.01.T01 AT3G01540 33.176 425 265 9 116 534 134 545 5.12e-72 241
MsG0680031717.01.T01 AT3G01540 33.176 425 265 9 116 534 134 545 5.12e-72 241
MsG0680031717.01.T01 AT3G58510 33.171 410 248 6 132 524 144 544 1.70e-71 240
MsG0680031717.01.T01 AT3G58510 33.171 410 248 6 132 524 144 544 1.70e-71 240
MsG0680031717.01.T01 AT3G58510 33.171 410 248 6 132 524 144 544 1.70e-71 240
MsG0680031717.01.T01 AT2G33730 31.765 425 257 7 121 520 294 710 2.85e-66 228
MsG0680031717.01.T01 AT3G06480 32.673 404 256 7 120 516 415 809 2.26e-65 230
MsG0680031717.01.T01 AT5G14610 33.162 389 243 8 154 537 45 421 4.53e-64 218
MsG0680031717.01.T01 AT5G14610 33.162 389 243 8 154 537 45 421 4.53e-64 218
MsG0680031717.01.T01 AT5G14610 33.162 389 243 8 154 537 244 620 7.17e-63 219
MsG0680031717.01.T01 AT4G33370 32.333 433 276 10 118 537 75 503 4.89e-62 213
MsG0680031717.01.T01 AT5G51280 31.773 406 263 7 118 513 124 525 1.03e-61 213
MsG0680031717.01.T01 AT1G31970 34.278 388 246 7 136 517 113 497 1.53e-60 209
MsG0680031717.01.T01 AT5G14610 33.244 373 230 9 111 477 140 499 3.32e-57 199
MsG0680031717.01.T01 AT2G47330 30.446 404 265 7 123 518 211 606 4.02e-56 201
MsG0680031717.01.T01 AT1G28180 33.333 360 218 7 167 516 1 348 9.47e-56 191
MsG0680031717.01.T01 AT5G63120 31.672 341 224 7 121 457 146 481 3.45e-55 193
MsG0680031717.01.T01 AT1G20920 29.657 408 274 7 116 516 167 568 2.15e-54 197
MsG0680031717.01.T01 AT5G63120 31.532 333 221 6 121 450 146 474 3.03e-54 191
MsG0680031717.01.T01 AT5G63120 31.532 333 221 6 121 450 146 474 3.03e-54 191
MsG0680031717.01.T01 AT5G63120 31.532 333 221 6 121 450 146 474 3.03e-54 191
MsG0680031717.01.T01 AT3G09720 30.170 411 258 9 121 518 118 512 6.13e-54 191
MsG0680031717.01.T01 AT3G09720 30.170 411 258 9 121 518 118 512 6.13e-54 191
MsG0680031717.01.T01 AT1G20920 29.657 408 274 7 116 516 505 906 8.56e-54 197
MsG0680031717.01.T01 AT1G20920 29.657 408 274 7 116 516 505 906 8.56e-54 197
MsG0680031717.01.T01 AT1G20920 29.657 408 274 7 116 516 505 906 8.56e-54 197
MsG0680031717.01.T01 AT1G20920 29.657 408 274 7 116 516 505 906 8.56e-54 197
MsG0680031717.01.T01 AT1G20920 29.657 408 274 7 116 516 505 906 8.56e-54 197
MsG0680031717.01.T01 AT1G20920 29.657 408 274 7 116 516 505 906 8.56e-54 197
MsG0680031717.01.T01 AT4G16630 30.728 371 243 7 134 498 162 524 2.19e-52 191
MsG0680031717.01.T01 AT3G06480 32.378 349 220 7 120 461 415 754 4.90e-52 190
MsG0680031717.01.T01 AT4G00660 30.130 385 251 6 141 521 133 503 1.96e-50 181
MsG0680031717.01.T01 AT4G00660 30.130 385 251 6 141 521 133 503 1.96e-50 181
MsG0680031717.01.T01 AT1G72730 32.143 364 235 5 140 501 42 395 4.79e-50 177
MsG0680031717.01.T01 AT3G13920 32.603 365 232 7 140 501 30 383 1.11e-49 176
MsG0680031717.01.T01 AT3G13920 32.603 365 232 7 140 501 40 393 1.15e-49 176
MsG0680031717.01.T01 AT3G13920 32.873 362 229 7 140 498 44 394 1.31e-49 176
MsG0680031717.01.T01 AT1G51380 30.252 357 235 7 137 490 20 365 1.34e-49 176
MsG0680031717.01.T01 AT3G13920 32.873 362 229 7 140 498 40 390 1.47e-49 176
MsG0680031717.01.T01 AT3G19760 27.027 370 252 5 137 501 33 389 1.83e-48 173
MsG0680031717.01.T01 AT1G54270 33.333 363 227 8 140 498 40 391 1.00e-47 171
MsG0680031717.01.T01 AT3G61240 31.476 359 228 6 141 495 126 470 6.51e-47 171
MsG0680031717.01.T01 AT3G61240 31.476 359 228 6 141 495 126 470 6.51e-47 171
MsG0680031717.01.T01 AT5G60990 29.563 389 256 7 138 515 8 389 8.15e-47 170
MsG0680031717.01.T01 AT3G13920 32.055 365 229 8 140 501 40 388 1.02e-46 168
MsG0680031717.01.T01 AT1G77030 29.775 356 242 4 141 494 30 379 4.27e-46 173
MsG0680031717.01.T01 AT3G09620 28.788 396 244 7 123 513 379 741 7.41e-46 173
MsG0680031717.01.T01 AT3G09620 28.788 396 244 7 123 513 380 742 7.63e-46 173
MsG0680031717.01.T01 AT5G26742 31.165 369 243 7 139 501 102 465 7.64e-46 172
MsG0680031717.01.T01 AT5G26742 31.165 369 243 7 139 501 102 465 7.70e-46 172
MsG0680031717.01.T01 AT2G45810 30.919 359 230 6 141 495 156 500 1.05e-45 168
MsG0680031717.01.T01 AT1G54270 32.782 363 224 9 140 498 40 386 1.21e-44 163
MsG0680031717.01.T01 AT3G22330 28.375 363 250 8 139 497 104 460 6.19e-44 165
MsG0680031717.01.T01 AT3G22310 29.396 364 245 9 139 497 116 472 6.16e-43 162
MsG0680031717.01.T01 AT3G22310 29.396 364 245 9 139 497 116 472 7.33e-43 162
MsG0680031717.01.T01 AT1G16280 30.939 362 233 8 140 494 59 410 1.35e-42 159
MsG0680031717.01.T01 AT5G26742 30.894 369 232 9 139 501 21 372 1.58e-41 159
MsG0680031717.01.T01 AT2G40700 29.291 437 232 13 140 501 31 465 2.13e-41 157
MsG0680031717.01.T01 AT5G11170 29.157 415 269 11 114 521 28 424 3.84e-39 148
MsG0680031717.01.T01 AT5G11200 29.157 415 269 11 114 521 28 424 3.84e-39 148
MsG0680031717.01.T01 AT1G71370 28.291 357 238 9 146 490 23 373 1.14e-37 146
MsG0680031717.01.T01 AT4G09730 25.065 387 274 6 138 510 111 495 1.28e-36 144
MsG0680031717.01.T01 AT3G18600 28.608 388 258 10 131 513 83 456 5.79e-35 139
MsG0680031717.01.T01 AT5G05450 26.997 363 236 10 150 490 27 382 3.16e-34 137
MsG0680031717.01.T01 AT5G62190 27.019 359 243 6 150 497 107 457 3.52e-34 137
MsG0680031717.01.T01 AT5G65900 28.906 384 251 10 140 515 155 524 6.82e-34 136
MsG0680031717.01.T01 AT5G08620 28.647 377 234 14 151 504 92 456 1.83e-33 134
MsG0680031717.01.T01 AT5G11200 26.696 457 267 13 114 521 28 465 2.84e-33 132
MsG0680031717.01.T01 AT5G54910 26.598 391 267 12 136 519 68 445 4.87e-32 131
MsG0680031717.01.T01 AT3G16840 26.157 432 244 13 140 501 191 617 9.54e-32 130
MsG0680031717.01.T01 AT3G16840 26.157 432 244 13 140 501 192 618 1.05e-31 130
MsG0680031717.01.T01 AT5G63630 27.520 367 251 11 151 504 66 430 3.66e-31 127
MsG0680031717.01.T01 AT5G63630 27.520 367 251 11 151 504 260 624 7.76e-31 127
MsG0680031717.01.T01 AT1G12770 26.225 408 246 12 140 495 111 515 5.81e-30 124
MsG0680031717.01.T01 AT1G12770 26.225 408 246 12 140 495 111 515 5.81e-30 124
MsG0680031717.01.T01 AT5G11170 29.191 346 227 10 183 521 7 341 2.03e-29 119
MsG0680031717.01.T01 AT5G08610 28.686 373 211 14 151 490 394 744 2.45e-29 123
MsG0680031717.01.T01 AT1G51380 28.395 243 163 5 137 378 20 252 5.80e-28 113
MsG0680031717.01.T01 AT4G34910 26.615 387 233 11 140 479 47 429 8.46e-28 118
MsG0680031717.01.T01 AT3G06980 24.603 378 255 9 140 490 375 749 8.34e-27 115
MsG0680031717.01.T01 AT3G53110 25.935 401 255 13 121 493 68 454 1.09e-26 114
MsG0680031717.01.T01 AT1G51380 29.108 213 143 4 137 348 20 225 1.78e-25 107
MsG0680031717.01.T01 AT1G63250 26.810 373 247 11 151 504 340 705 2.32e-25 111
MsG0680031717.01.T01 AT1G71280 27.302 315 191 9 146 452 24 308 2.37e-25 107
MsG0680031717.01.T01 AT1G51380 29.108 213 143 4 137 348 20 225 2.48e-25 106
MsG0680031717.01.T01 AT1G71280 27.358 318 193 9 146 455 24 311 2.88e-25 109
MsG0680031717.01.T01 AT2G07750 26.542 373 248 11 151 504 387 752 7.60e-24 106
MsG0680031717.01.T01 AT5G19210 25.138 362 233 9 146 479 83 434 1.99e-23 103
MsG0680031717.01.T01 AT4G15850 23.326 433 254 11 149 513 41 463 3.54e-21 97.4
MsG0680031717.01.T01 AT1G28180 36.441 118 70 2 167 281 1 116 1.45e-18 82.0
MsG0680031717.01.T01 AT5G11200 28.205 234 148 7 114 342 28 246 5.31e-16 81.3
MsG0680031717.01.T01 AT5G11200 29.208 202 138 4 321 521 286 483 1.69e-14 76.6
MsG0680031717.01.T01 AT1G10930 26.184 359 220 15 151 489 443 776 4.37e-15 79.3
MsG0680031717.01.T01 AT1G27880 24.011 354 227 12 158 494 266 594 8.40e-14 75.1
MsG0680031717.01.T01 AT1G27880 24.079 353 226 12 158 493 266 593 1.27e-13 74.3
MsG0680031717.01.T01 AT3G05740 24.804 383 242 15 146 508 106 462 1.11e-12 70.9
MsG0680031717.01.T01 AT3G05740 25.065 383 241 15 146 508 191 547 2.13e-12 70.1
MsG0680031717.01.T01 AT4G35740 23.373 338 206 12 166 479 41 349 6.31e-11 65.5

Find 131 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGTGGCTTAAAATGTTTC+TGG 0.147612 6:+25243690 None:intergenic
CTTGCACAACGAGATATTAT+TGG 0.230162 6:+25244244 None:intergenic
GGTTGTGGCTACTGGAGTTT+TGG 0.244181 6:-25243513 MsG0680031717.01.T01:CDS
AGAAATAATGCAGTCATTTA+TGG 0.244375 6:-25243552 MsG0680031717.01.T01:CDS
CGACATGCCTAATTCGATTA+AGG 0.248513 6:-25243447 MsG0680031717.01.T01:CDS
TTCCCGGTGGTTGTGGCTAC+TGG 0.281226 6:-25243521 MsG0680031717.01.T01:CDS
GACCAGAGAGATGCTCTTCC+TGG 0.301895 6:-25244733 MsG0680031717.01.T01:CDS
TGATGATGATGTTTGTAGTT+TGG 0.317671 6:-25244656 MsG0680031717.01.T01:CDS
CTGCATTATTTCTCTCCTTT+CGG 0.320408 6:+25243562 None:intergenic
CCGTGAAACAGCTAGCAATT+TGG 0.321173 6:-25243760 MsG0680031717.01.T01:CDS
ACGGCCTTGTTTGGTCTGTT+AGG 0.325864 6:+25243779 None:intergenic
CTTTCAACCGATTCACCTAA+TGG 0.337119 6:-25246157 MsG0680031717.01.T01:intron
AGCAATCCCCGCTGCTTTGA+TGG 0.339953 6:-25244326 MsG0680031717.01.T01:CDS
CGTGAAACAGCTAGCAATTT+GGG 0.341262 6:-25243759 MsG0680031717.01.T01:CDS
AAGGAATATGTACATCAAAT+AGG 0.341270 6:-25243428 MsG0680031717.01.T01:CDS
TCCAGCACTGGATGAGGATT+TGG 0.344408 6:+25244603 None:intergenic
GGGGAAGAACATGCTTGTTT+TGG 0.352453 6:-25244305 MsG0680031717.01.T01:CDS
ATGTTCTGCAGGAGTTTGTC+AGG 0.352782 6:+25244391 None:intergenic
AGCTGTTTCACGGCCTTGTT+TGG 0.357465 6:+25243770 None:intergenic
GGTAGAGGGGTTGATCTATT+AGG 0.360776 6:-25243491 MsG0680031717.01.T01:CDS
ACATATTCCTTAATCGAATT+AGG 0.361620 6:+25243440 None:intergenic
TCATGTGAAGGGTGTTGTTT+CGG 0.371484 6:-25244446 MsG0680031717.01.T01:CDS
AGAGAAGATGGCAAACTCTA+TGG 0.375037 6:-25243834 MsG0680031717.01.T01:CDS
TCATCGTTTGATGTATGTTT+CGG 0.378578 6:-25244221 MsG0680031717.01.T01:CDS
GTCCGATTCTTTAGAGAAGA+TGG 0.393381 6:-25243846 MsG0680031717.01.T01:CDS
CGATGTCAAGGTGAGTTCTA+AGG 0.394116 6:-25244755 MsG0680031717.01.T01:CDS
ATTTATGGTCGGCGAGTTCC+CGG 0.396686 6:-25243537 MsG0680031717.01.T01:CDS
CACGCGAAATTGCTCGGAAA+AGG 0.410354 6:-25244133 MsG0680031717.01.T01:CDS
GGGATTGCTTGCATTTGAAC+AGG 0.411047 6:+25244340 None:intergenic
AAATCAGGAACGCCGTCTTT+AGG 0.411740 6:-25244514 MsG0680031717.01.T01:CDS
CGGGTGCCGCCGCTGTACCT+CGG 0.418581 6:-25243310 MsG0680031717.01.T01:CDS
TTTAGGAGGTGATCAGGTTC+AGG 0.420816 6:-25244497 MsG0680031717.01.T01:CDS
GAAAGCGATTGAAACTTTGA+TGG 0.426209 6:+25246175 None:intergenic
CGGCGAGTTCCCGGTGGTTG+TGG 0.434782 6:-25243528 MsG0680031717.01.T01:CDS
TATACGTCGGCTCGAGACTC+GGG 0.438739 6:-25243661 MsG0680031717.01.T01:CDS
AATAGGAAGGGCATCTAGAA+TGG 0.439220 6:-25243411 MsG0680031717.01.T01:CDS
AACATTACCATTAGGTGAAT+CGG 0.441100 6:+25246150 None:intergenic
TGCCATCTTCTCTAAAGAAT+CGG 0.441703 6:+25243844 None:intergenic
GTTAAAGCTGTTTCAATTCA+CGG 0.442461 6:-25243599 MsG0680031717.01.T01:CDS
CCCCAGGAAGAGCATCTCTC+TGG 0.445942 6:+25244731 None:intergenic
AACGCAATTACAGTTTCAAC+AGG 0.446108 6:-25243623 MsG0680031717.01.T01:CDS
GCAATCCCCGCTGCTTTGAT+GGG 0.456850 6:-25244325 MsG0680031717.01.T01:CDS
GAGACTCGGGGCAGATCTCT+TGG 0.456902 6:-25243648 MsG0680031717.01.T01:CDS
CAGCTGCTTCTATGTTCTGC+AGG 0.459617 6:+25244380 None:intergenic
GCCGTCTTTAGGAGGTGATC+AGG 0.459928 6:-25244503 MsG0680031717.01.T01:CDS
AGAGATACTGACAACAAATC+AGG 0.465586 6:-25244529 MsG0680031717.01.T01:CDS
CACGGCTTTGTCACAACCTC+AGG 0.465662 6:-25243891 MsG0680031717.01.T01:CDS
ACCAGAGAGATGCTCTTCCT+GGG 0.473413 6:-25244732 MsG0680031717.01.T01:CDS
AAGTGGACTGCATGCTTCAA+AGG 0.474942 6:-25243943 MsG0680031717.01.T01:CDS
TTCTTCCCCATCAAAGCAGC+GGG 0.475720 6:+25244319 None:intergenic
TAGTTTGGAATGTAAACAAT+CGG 0.486405 6:-25244641 MsG0680031717.01.T01:CDS
CATGAAGCAAACGACATAAC+CGG 0.487625 6:+25244421 None:intergenic
ACAAGGAGTTGAATTGATTG+TGG 0.487874 6:-25244044 MsG0680031717.01.T01:CDS
CAAGGAGTTGAATTGATTGT+GGG 0.488532 6:-25244043 MsG0680031717.01.T01:CDS
GGGTGCCGCCGCTGTACCTC+GGG 0.488624 6:-25243309 MsG0680031717.01.T01:CDS
ACAATACACTGGGGCTCCCC+AGG 0.494415 6:+25244715 None:intergenic
CCAAATTGCTAGCTGTTTCA+CGG 0.499554 6:+25243760 None:intergenic
TACGAGAGAGCTCAGTATAC+AGG 0.499894 6:-25244164 MsG0680031717.01.T01:CDS
TTTAAAACTGCACTTGTGGT+TGG 0.501585 6:-25244103 MsG0680031717.01.T01:CDS
AAACTTTGATGGGTCATCGT+TGG 0.503229 6:+25246186 None:intergenic
AGTGGACTGCATGCTTCAAA+GGG 0.503807 6:-25243942 MsG0680031717.01.T01:CDS
TAAATGCACTCGTCGGCTGC+AGG 0.504410 6:+25244556 None:intergenic
ACTCCAGTAGCCACAACCAC+CGG 0.504693 6:+25243518 None:intergenic
GAGCCGACGTATATTACAAC+AGG 0.508711 6:+25243671 None:intergenic
GAAGAGCACGCGAAATTGCT+CGG 0.511225 6:-25244139 MsG0680031717.01.T01:CDS
TCAGGTATTGCAGATATTCA+CGG 0.515977 6:-25243909 MsG0680031717.01.T01:CDS
ATATACGTCGGCTCGAGACT+CGG 0.519620 6:-25243662 MsG0680031717.01.T01:CDS
AAAAGATCAACAAGCCGTCC+TGG 0.520031 6:+25244016 None:intergenic
TAAAGAATCGGACGTTGTTG+CGG 0.524657 6:+25243856 None:intergenic
GAATTGATTGTGGGAACACC+AGG 0.524874 6:-25244034 MsG0680031717.01.T01:CDS
ATAGGAAGGGCATCTAGAAT+GGG 0.525349 6:-25243410 MsG0680031717.01.T01:CDS
GTTAGTCGATGTATTAAAGT+CGG 0.529271 6:-25243330 MsG0680031717.01.T01:CDS
AAAACTGCACTTGTGGTTGG+TGG 0.533633 6:-25244100 MsG0680031717.01.T01:CDS
AGACTTTCCTGAACCTGTAT+CGG 0.533821 6:+25244282 None:intergenic
GAAGGGTGTTGTTTCGGCAC+CGG 0.534256 6:-25244440 MsG0680031717.01.T01:CDS
CACATATATATTCACCGTAT+CGG 0.534333 6:+25244686 None:intergenic
AATATGTACATCAAATAGGA+AGG 0.538019 6:-25243424 MsG0680031717.01.T01:CDS
TCCAAATCCTCATCCAGTGC+TGG 0.543059 6:-25244604 MsG0680031717.01.T01:CDS
TGTGGTTGGTGGTGATGCCA+TGG 0.551178 6:-25244089 MsG0680031717.01.T01:CDS
TTAGTCGATGTATTAAAGTC+GGG 0.558344 6:-25243329 MsG0680031717.01.T01:CDS
GAAACTCGACATTCATGTGA+AGG 0.558611 6:-25244458 MsG0680031717.01.T01:CDS
AAAGCGATTGAAACTTTGAT+GGG 0.561237 6:+25246176 None:intergenic
AAGGGCATCTAGAATGGGAG+AGG 0.566272 6:-25243405 MsG0680031717.01.T01:CDS
CTGCAGAACATAGAAGCAGC+TGG 0.566645 6:-25244379 MsG0680031717.01.T01:CDS
CTCCAGTAGCCACAACCACC+GGG 0.567240 6:+25243519 None:intergenic
GCTTGCATTTGAACAGGCGT+AGG 0.569429 6:+25244346 None:intergenic
GATACAGTGGTTATAACCGT+TGG 0.572374 6:-25243806 MsG0680031717.01.T01:CDS
TGTATTGTATGTGGCCGATA+CGG 0.574833 6:-25244700 MsG0680031717.01.T01:CDS
AAGACATCCAGCACTGGATG+AGG 0.577695 6:+25244597 None:intergenic
TGTTCTGCAGGAGTTTGTCA+GGG 0.581419 6:+25244392 None:intergenic
TCAGGAACGCCGTCTTTAGG+AGG 0.581955 6:-25244511 MsG0680031717.01.T01:CDS
TGAACCTAACAGACCAAACA+AGG 0.584747 6:-25243783 MsG0680031717.01.T01:CDS
GCATCTAGAATGGGAGAGGA+AGG 0.588001 6:-25243401 MsG0680031717.01.T01:CDS
ATCGGCCACATACAATACAC+TGG 0.590795 6:+25244704 None:intergenic
CAACTTTACCGCATTCAACA+AGG 0.593925 6:-25244061 MsG0680031717.01.T01:CDS
GCGGTAAAGTTGTCTAGCCA+TGG 0.598297 6:+25244072 None:intergenic
TGGTGGAAGACATCCAGCAC+TGG 0.602023 6:+25244591 None:intergenic
AGATGCAGGGAACGATGTCA+AGG 0.602458 6:-25244767 MsG0680031717.01.T01:CDS
TGGTCTGTTAGGTTCACCAA+CGG 0.604355 6:+25243790 None:intergenic
ATTCAACTCCTTGTTGAATG+CGG 0.607306 6:+25244053 None:intergenic
TCGGCCACATACAATACACT+GGG 0.609672 6:+25244705 None:intergenic
GAGCCCCAGTGTATTGTATG+TGG 0.610734 6:-25244709 MsG0680031717.01.T01:CDS
CTCTATGGCAAAAGATACAG+TGG 0.614077 6:-25243819 MsG0680031717.01.T01:CDS
GCTGTTTAAAACTGCACTTG+TGG 0.617927 6:-25244107 MsG0680031717.01.T01:CDS
AAACTCGACATTCATGTGAA+GGG 0.624831 6:-25244457 MsG0680031717.01.T01:CDS
GCGAGAAATCTATGACCGAA+AGG 0.633150 6:-25243577 MsG0680031717.01.T01:CDS
ACCTGATCACCTCCTAAAGA+CGG 0.633787 6:+25244502 None:intergenic
CAATCCCCGCTGCTTTGATG+GGG 0.635262 6:-25244324 MsG0680031717.01.T01:CDS
TCCTCCAGAGAAAACCCTTG+TGG 0.636938 6:+25243265 None:intergenic
GATAAGCTCCCGAGGTACAG+CGG 0.640895 6:+25243301 None:intergenic
ATATGTACATCAAATAGGAA+GGG 0.645395 6:-25243423 MsG0680031717.01.T01:CDS
GCGGAATACATTAAGACCTG+AGG 0.646184 6:+25243875 None:intergenic
ATACGTCGGCTCGAGACTCG+GGG 0.647161 6:-25243660 MsG0680031717.01.T01:CDS
TGTATACTGAGCTCTCTCGT+AGG 0.649466 6:+25244166 None:intergenic
CCGACGTATATTACAACAGG+TGG 0.652630 6:+25243674 None:intergenic
CCAGAGAGATGCTCTTCCTG+GGG 0.652795 6:-25244731 MsG0680031717.01.T01:CDS
ATAATGCAGTCATTTATGGT+CGG 0.657786 6:-25243548 MsG0680031717.01.T01:CDS
GTTCTTCCCCATCAAAGCAG+CGG 0.661215 6:+25244318 None:intergenic
TCTGCAGGAGTTTGTCAGGG+AGG 0.664045 6:+25244395 None:intergenic
GGTGTTAACACCATAGCTAG+AGG 0.666575 6:+25244187 None:intergenic
TGATTGTGGGAACACCAGGA+CGG 0.673152 6:-25244030 MsG0680031717.01.T01:CDS
CAATTCACAGTATACCACAA+GGG 0.675999 6:-25243279 MsG0680031717.01.T01:CDS
CCACCTGTTGTAATATACGT+CGG 0.679670 6:-25243674 MsG0680031717.01.T01:CDS
TCAATTCACAGTATACCACA+AGG 0.680822 6:-25243280 MsG0680031717.01.T01:CDS
TCTAACATAAATGCACTCGT+CGG 0.680838 6:+25244549 None:intergenic
TGTGAATTGATAAGCTCCCG+AGG 0.687454 6:+25243293 None:intergenic
TATGGTCGGCGAGTTCCCGG+TGG 0.702170 6:-25243534 MsG0680031717.01.T01:CDS
GTGGACTGCATGCTTCAAAG+GGG 0.709095 6:-25243941 MsG0680031717.01.T01:CDS
AAGCTCCCGAGGTACAGCGG+CGG 0.720985 6:+25243304 None:intergenic
TCTTCCCCATCAAAGCAGCG+GGG 0.769950 6:+25244320 None:intergenic
CGGCCACATACAATACACTG+GGG 0.805673 6:+25244706 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAAATAAACAAGATTAAT+TGG + Chr6:25244649-25244668 None:intergenic 15.0%
!!! CAATTTCATTTTTTTTTTCT+GGG - Chr6:25243348-25243367 MsG0680031717.01.T01:CDS 15.0%
!!! TCAATTTCATTTTTTTTTTC+TGG - Chr6:25243347-25243366 MsG0680031717.01.T01:CDS 15.0%
!! AGAAATTACAAAACAACTTA+TGG + Chr6:25243538-25243557 None:intergenic 20.0%
!! ATAAGTTGTTTATTCAAACA+TGG + Chr6:25243595-25243614 None:intergenic 20.0%
!! TATAATTCAGTGTTAACAAA+TGG - Chr6:25243425-25243444 MsG0680031717.01.T01:CDS 20.0%
!!! ATAATTGATTTTGACATGTT+TGG - Chr6:25243726-25243745 MsG0680031717.01.T01:CDS 20.0%
!!! TGTTTTCATATAAGATATCA+TGG + Chr6:25244336-25244355 None:intergenic 20.0%
!!! TTATTTTAGAGAACTTATGA+TGG - Chr6:25243489-25243508 MsG0680031717.01.T01:CDS 20.0%
! AAGGAATATGTACATCAAAT+AGG - Chr6:25246006-25246025 MsG0680031717.01.T01:intron 25.0%
! AATATGTACATCAAATAGGA+AGG - Chr6:25246010-25246029 MsG0680031717.01.T01:intron 25.0%
! AATTCCAAAAGTAGACAATT+TGG + Chr6:25243562-25243581 None:intergenic 25.0%
! ACATATTCCTTAATCGAATT+AGG + Chr6:25245997-25246016 None:intergenic 25.0%
! AGAAATAATGCAGTCATTTA+TGG - Chr6:25245882-25245901 MsG0680031717.01.T01:intron 25.0%
! ATATGTACATCAAATAGGAA+GGG - Chr6:25246011-25246030 MsG0680031717.01.T01:intron 25.0%
! ATTCCAAAAGTAGACAATTT+GGG + Chr6:25243561-25243580 None:intergenic 25.0%
! GGATGTAAAATTGATTATAG+AGG - Chr6:25243700-25243719 MsG0680031717.01.T01:CDS 25.0%
! TCAAATATGACAATCCAAAT+GGG + Chr6:25244210-25244229 None:intergenic 25.0%
! TGAAGAATAGATTTCAATAC+AGG + Chr6:25243323-25243342 None:intergenic 25.0%
!! AATTGATTCTAGTTGAAGTT+AGG - Chr6:25243781-25243800 MsG0680031717.01.T01:CDS 25.0%
!! TAGTTTGGAATGTAAACAAT+CGG - Chr6:25244793-25244812 MsG0680031717.01.T01:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+TGG - Chr6:25244425-25244444 MsG0680031717.01.T01:CDS 25.0%
!!! AAAACCCTTTTTGGAATTTT+AGG - Chr6:25244069-25244088 MsG0680031717.01.T01:CDS 25.0%
!!! AAACCCTTTTTGGAATTTTA+GGG - Chr6:25244070-25244089 MsG0680031717.01.T01:CDS 25.0%
!!! AATTCACTTTTAGATGGAAT+CGG - Chr6:25243856-25243875 MsG0680031717.01.T01:CDS 25.0%
!!! ATCTTGTTTATTTTAGATGC+AGG - Chr6:25244653-25244672 MsG0680031717.01.T01:CDS 25.0%
!!! ATTGTTTCTCTAGTGAATTT+TGG - Chr6:25244534-25244553 MsG0680031717.01.T01:CDS 25.0%
!!! ATTTTTGAAAACAACCCATT+TGG - Chr6:25244193-25244212 MsG0680031717.01.T01:CDS 25.0%
!!! CAATTGCTGTTTTCAAATTT+TGG - Chr6:25243392-25243411 MsG0680031717.01.T01:CDS 25.0%
!!! CATAAGTTTTGTGAAAGTAT+TGG + Chr6:25244038-25244057 None:intergenic 25.0%
!!! GTCTACTTTTGGAATTATAA+GGG - Chr6:25243566-25243585 MsG0680031717.01.T01:CDS 25.0%
!!! TCTTGTTTATTTTAGATGCA+GGG - Chr6:25244654-25244673 MsG0680031717.01.T01:CDS 25.0%
!!! TGTCTACTTTTGGAATTATA+AGG - Chr6:25243565-25243584 MsG0680031717.01.T01:CDS 25.0%
!!! TTGTTCAATTCACTTTTAGA+TGG - Chr6:25243850-25243869 MsG0680031717.01.T01:CDS 25.0%
AAAGCGATTGAAACTTTGAT+GGG + Chr6:25243261-25243280 None:intergenic 30.0%
AACATTACCATTAGGTGAAT+CGG + Chr6:25243287-25243306 None:intergenic 30.0%
ATAATGCAGTCATTTATGGT+CGG - Chr6:25245886-25245905 MsG0680031717.01.T01:intron 30.0%
CACATATATATTCACCGTAT+CGG + Chr6:25244751-25244770 None:intergenic 30.0%
CTACAAATACCTTATCAACA+TGG + Chr6:25244295-25244314 None:intergenic 30.0%
CTCAAATATGACAATCCAAA+TGG + Chr6:25244211-25244230 None:intergenic 30.0%
CTCATCGTTTACAAAAACTA+AGG + Chr6:25246064-25246083 None:intergenic 30.0%
CTTAGCAAACTCATGAAAAT+TGG - Chr6:25243964-25243983 MsG0680031717.01.T01:CDS 30.0%
GAATTAGGAACATTACCATT+AGG + Chr6:25243295-25243314 None:intergenic 30.0%
GTTAGTCGATGTATTAAAGT+CGG - Chr6:25246104-25246123 MsG0680031717.01.T01:intron 30.0%
TAGCCCTAAAATTCCAAAAA+GGG + Chr6:25244076-25244095 None:intergenic 30.0%
TCAACTAGAATCAATTCTTG+CGG + Chr6:25243777-25243796 None:intergenic 30.0%
TCATCGTTTGATGTATGTTT+CGG - Chr6:25245213-25245232 MsG0680031717.01.T01:intron 30.0%
TTAGTCGATGTATTAAAGTC+GGG - Chr6:25246105-25246124 MsG0680031717.01.T01:intron 30.0%
! AATTTTACATCCTCCAAAAG+TGG + Chr6:25243692-25243711 None:intergenic 30.0%
! AGTAATGAGAAAACCCTTTT+TGG - Chr6:25244060-25244079 MsG0680031717.01.T01:CDS 30.0%
! GTTAAAGCTGTTTCAATTCA+CGG - Chr6:25245835-25245854 MsG0680031717.01.T01:intron 30.0%
! TGATGATGATGTTTGTAGTT+TGG - Chr6:25244778-25244797 MsG0680031717.01.T01:intron 30.0%
! TGTATGTTTGATTCCACTTT+TGG - Chr6:25243676-25243695 MsG0680031717.01.T01:CDS 30.0%
AAACTCGACATTCATGTGAA+GGG - Chr6:25244977-25244996 MsG0680031717.01.T01:intron 35.0%
AACGCAATTACAGTTTCAAC+AGG - Chr6:25245811-25245830 MsG0680031717.01.T01:intron 35.0%
ACAAGGAGTTGAATTGATTG+TGG - Chr6:25245390-25245409 MsG0680031717.01.T01:intron 35.0%
AGAAAGATTGATCACTCTAC+TGG + Chr6:25244493-25244512 None:intergenic 35.0%
AGAGATACTGACAACAAATC+AGG - Chr6:25244905-25244924 MsG0680031717.01.T01:intron 35.0%
ATTCAACTCCTTGTTGAATG+CGG + Chr6:25245384-25245403 None:intergenic 35.0%
CAAGGAGTTGAATTGATTGT+GGG - Chr6:25245391-25245410 MsG0680031717.01.T01:intron 35.0%
CAATTCACAGTATACCACAA+GGG - Chr6:25246155-25246174 MsG0680031717.01.T01:intron 35.0%
CTAGCCCTAAAATTCCAAAA+AGG + Chr6:25244077-25244096 None:intergenic 35.0%
CTTGCACAACGAGATATTAT+TGG + Chr6:25245193-25245212 None:intergenic 35.0%
GAAAGCGATTGAAACTTTGA+TGG + Chr6:25243262-25243281 None:intergenic 35.0%
TAACAAAAAGCCTCTAGCTA+TGG - Chr6:25245237-25245256 MsG0680031717.01.T01:intron 35.0%
TCAATACAGGAAGACGAATT+AGG + Chr6:25243310-25243329 None:intergenic 35.0%
TCAATTCACAGTATACCACA+AGG - Chr6:25246154-25246173 MsG0680031717.01.T01:intron 35.0%
TCTAACATAAATGCACTCGT+CGG + Chr6:25244888-25244907 None:intergenic 35.0%
! CTGCATTATTTCTCTCCTTT+CGG + Chr6:25245875-25245894 None:intergenic 35.0%
! TCAGGTATTGCAGATATTCA+CGG - Chr6:25245525-25245544 MsG0680031717.01.T01:intron 35.0%
! TCTCCCAAATTGTCTACTTT+TGG - Chr6:25243555-25243574 MsG0680031717.01.T01:CDS 35.0%
! TGCCATCTTCTCTAAAGAAT+CGG + Chr6:25245593-25245612 None:intergenic 35.0%
! TTTAAAACTGCACTTGTGGT+TGG - Chr6:25245331-25245350 MsG0680031717.01.T01:intron 35.0%
!! ATGTTTGATTCCACTTTTGG+AGG - Chr6:25243679-25243698 MsG0680031717.01.T01:CDS 35.0%
AGACTTTCCTGAACCTGTAT+CGG + Chr6:25245155-25245174 None:intergenic 40.0%
AGAGAAGATGGCAAACTCTA+TGG - Chr6:25245600-25245619 MsG0680031717.01.T01:intron 40.0%
CAACTTTACCGCATTCAACA+AGG - Chr6:25245373-25245392 MsG0680031717.01.T01:intron 40.0%
CAGGTGGCTTAAAATGTTTC+TGG + Chr6:25245747-25245766 None:intergenic 40.0%
CATGAAGCAAACGACATAAC+CGG + Chr6:25245016-25245035 None:intergenic 40.0%
CCAAACATGGTTGTTACTGT+TGG - Chr6:25244438-25244457 MsG0680031717.01.T01:CDS 40.0%
CCAACAGTAACAACCATGTT+TGG + Chr6:25244441-25244460 None:intergenic 40.0%
CCACCTGTTGTAATATACGT+CGG - Chr6:25245760-25245779 MsG0680031717.01.T01:intron 40.0%
CGACATGCCTAATTCGATTA+AGG - Chr6:25245987-25246006 MsG0680031717.01.T01:intron 40.0%
CGTGAAACAGCTAGCAATTT+GGG - Chr6:25245675-25245694 MsG0680031717.01.T01:intron 40.0%
CTCTATGGCAAAAGATACAG+TGG - Chr6:25245615-25245634 MsG0680031717.01.T01:intron 40.0%
GAAACTCGACATTCATGTGA+AGG - Chr6:25244976-25244995 MsG0680031717.01.T01:intron 40.0%
GATACAGTGGTTATAACCGT+TGG - Chr6:25245628-25245647 MsG0680031717.01.T01:intron 40.0%
GTCGTTATTGCGGAAAATTG+TGG - Chr6:25244586-25244605 MsG0680031717.01.T01:CDS 40.0%
GTGTTGCATTGTCGTTATTG+CGG - Chr6:25244576-25244595 MsG0680031717.01.T01:CDS 40.0%
TAAAGAATCGGACGTTGTTG+CGG + Chr6:25245581-25245600 None:intergenic 40.0%
TAAGCACTTGTGAGGCTATT+GGG - Chr6:25244245-25244264 MsG0680031717.01.T01:CDS 40.0%
TAGCTTACGCCATGTTGATA+AGG - Chr6:25244283-25244302 MsG0680031717.01.T01:CDS 40.0%
TATTAGCGTAAGCACTTGTG+AGG - Chr6:25244237-25244256 MsG0680031717.01.T01:CDS 40.0%
TGAACCTAACAGACCAAACA+AGG - Chr6:25245651-25245670 MsG0680031717.01.T01:intron 40.0%
TGTATTGTATGTGGCCGATA+CGG - Chr6:25244734-25244753 MsG0680031717.01.T01:CDS 40.0%
! AAACTTTGATGGGTCATCGT+TGG + Chr6:25243251-25243270 None:intergenic 40.0%
! CCAAATTGCTAGCTGTTTCA+CGG + Chr6:25245677-25245696 None:intergenic 40.0%
! CTTTCAACCGATTCACCTAA+TGG - Chr6:25243277-25243296 MsG0680031717.01.T01:CDS 40.0%
! GCTGTTTAAAACTGCACTTG+TGG - Chr6:25245327-25245346 MsG0680031717.01.T01:intron 40.0%
! GTCCGATTCTTTAGAGAAGA+TGG - Chr6:25245588-25245607 MsG0680031717.01.T01:intron 40.0%
! TATACCACAAGGGTTTTCTC+TGG - Chr6:25246165-25246184 MsG0680031717.01.T01:CDS 40.0%
!! AATAGGAAGGGCATCTAGAA+TGG - Chr6:25246023-25246042 MsG0680031717.01.T01:intron 40.0%
!! ATAGGAAGGGCATCTAGAAT+GGG - Chr6:25246024-25246043 MsG0680031717.01.T01:intron 40.0%
!! GACTTTTGTTGTCGATGAAG+TGG - Chr6:25245474-25245493 MsG0680031717.01.T01:intron 40.0%
!! TCATGTGAAGGGTGTTGTTT+CGG - Chr6:25244988-25245007 MsG0680031717.01.T01:intron 40.0%
!!! GTGAATTTTGGTGCCATGAT+TGG - Chr6:25244546-25244565 MsG0680031717.01.T01:CDS 40.0%
AAAACTGCACTTGTGGTTGG+TGG - Chr6:25245334-25245353 MsG0680031717.01.T01:intron 45.0%
AAAAGATCAACAAGCCGTCC+TGG + Chr6:25245421-25245440 None:intergenic 45.0%
AAATCAGGAACGCCGTCTTT+AGG - Chr6:25244920-25244939 MsG0680031717.01.T01:intron 45.0%
AAGCACTTGTGAGGCTATTG+GGG - Chr6:25244246-25244265 MsG0680031717.01.T01:CDS 45.0%
ACCTGATCACCTCCTAAAGA+CGG + Chr6:25244935-25244954 None:intergenic 45.0%
ATCGGCCACATACAATACAC+TGG + Chr6:25244733-25244752 None:intergenic 45.0%
ATGTTCTGCAGGAGTTTGTC+AGG + Chr6:25245046-25245065 None:intergenic 45.0%
CAACACATCGCAACCAATCA+TGG + Chr6:25244562-25244581 None:intergenic 45.0%
CCGACGTATATTACAACAGG+TGG + Chr6:25245763-25245782 None:intergenic 45.0%
CCGTGAAACAGCTAGCAATT+TGG - Chr6:25245674-25245693 MsG0680031717.01.T01:intron 45.0%
CGATGTCAAGGTGAGTTCTA+AGG - Chr6:25244679-25244698 MsG0680031717.01.T01:CDS 45.0%
GAATTGATTGTGGGAACACC+AGG - Chr6:25245400-25245419 MsG0680031717.01.T01:intron 45.0%
GAGCCGACGTATATTACAAC+AGG + Chr6:25245766-25245785 None:intergenic 45.0%
GCGAGAAATCTATGACCGAA+AGG - Chr6:25245857-25245876 MsG0680031717.01.T01:intron 45.0%
GCGGAATACATTAAGACCTG+AGG + Chr6:25245562-25245581 None:intergenic 45.0%
GGGATTGCTTGCATTTGAAC+AGG + Chr6:25245097-25245116 None:intergenic 45.0%
GGTGTTAACACCATAGCTAG+AGG + Chr6:25245250-25245269 None:intergenic 45.0%
GTAAGCACTTGTGAGGCTAT+TGG - Chr6:25244244-25244263 MsG0680031717.01.T01:CDS 45.0%
TACGAGAGAGCTCAGTATAC+AGG - Chr6:25245270-25245289 MsG0680031717.01.T01:intron 45.0%
TCGGCCACATACAATACACT+GGG + Chr6:25244732-25244751 None:intergenic 45.0%
TGGTCTGTTAGGTTCACCAA+CGG + Chr6:25245647-25245666 None:intergenic 45.0%
TGTATACTGAGCTCTCTCGT+AGG + Chr6:25245271-25245290 None:intergenic 45.0%
TGTGAATTGATAAGCTCCCG+AGG + Chr6:25246144-25246163 None:intergenic 45.0%
TGTTCTGCAGGAGTTTGTCA+GGG + Chr6:25245045-25245064 None:intergenic 45.0%
TTTAGGAGGTGATCAGGTTC+AGG - Chr6:25244937-25244956 MsG0680031717.01.T01:intron 45.0%
! GGGGAAGAACATGCTTGTTT+TGG - Chr6:25245129-25245148 MsG0680031717.01.T01:intron 45.0%
! GGTAGAGGGGTTGATCTATT+AGG - Chr6:25245943-25245962 MsG0680031717.01.T01:intron 45.0%
! GTTGTGGCTACTGGAGTTTT+GGG - Chr6:25245922-25245941 MsG0680031717.01.T01:intron 45.0%
!! AAGGGTTTTCTCTGGAGGAA+AGG - Chr6:25246173-25246192 MsG0680031717.01.T01:CDS 45.0%
!! AAGTGGACTGCATGCTTCAA+AGG - Chr6:25245491-25245510 MsG0680031717.01.T01:intron 45.0%
!! AGGGTTTTCTCTGGAGGAAA+GGG - Chr6:25246174-25246193 MsG0680031717.01.T01:CDS 45.0%
!! AGTGGACTGCATGCTTCAAA+GGG - Chr6:25245492-25245511 MsG0680031717.01.T01:intron 45.0%
!! TCAAAGGGGTTTTCGAGATC+AGG - Chr6:25245507-25245526 MsG0680031717.01.T01:intron 45.0%
!!! ATGCTTGTTTTGGCCGATAC+AGG - Chr6:25245139-25245158 MsG0680031717.01.T01:intron 45.0%
!!! CTACTGGAGTTTTGGGTAGA+GGG - Chr6:25245929-25245948 MsG0680031717.01.T01:intron 45.0%
!!! TACTGGAGTTTTGGGTAGAG+GGG - Chr6:25245930-25245949 MsG0680031717.01.T01:intron 45.0%
ACCAGAGAGATGCTCTTCCT+GGG - Chr6:25244702-25244721 MsG0680031717.01.T01:CDS 50.0%
AGATGCAGGGAACGATGTCA+AGG - Chr6:25244667-25244686 MsG0680031717.01.T01:CDS 50.0%
AGGCTATTGGGGAGAGCTTA+TGG - Chr6:25244257-25244276 MsG0680031717.01.T01:CDS 50.0%
ATTTATGGTCGGCGAGTTCC+CGG - Chr6:25245897-25245916 MsG0680031717.01.T01:intron 50.0%
CACGCGAAATTGCTCGGAAA+AGG - Chr6:25245301-25245320 MsG0680031717.01.T01:intron 50.0%
CAGCTGCTTCTATGTTCTGC+AGG + Chr6:25245057-25245076 None:intergenic 50.0%
CGGCCACATACAATACACTG+GGG + Chr6:25244731-25244750 None:intergenic 50.0%
CTGCAGAACATAGAAGCAGC+TGG - Chr6:25245055-25245074 MsG0680031717.01.T01:intron 50.0%
GAGCCCCAGTGTATTGTATG+TGG - Chr6:25244725-25244744 MsG0680031717.01.T01:CDS 50.0%
GCGGTAAAGTTGTCTAGCCA+TGG + Chr6:25245365-25245384 None:intergenic 50.0%
GCTTGCATTTGAACAGGCGT+AGG + Chr6:25245091-25245110 None:intergenic 50.0%
GTTCTTCCCCATCAAAGCAG+CGG + Chr6:25245119-25245138 None:intergenic 50.0%
TCCAAATCCTCATCCAGTGC+TGG - Chr6:25244830-25244849 MsG0680031717.01.T01:intron 50.0%
TCCTCCAGAGAAAACCCTTG+TGG + Chr6:25246172-25246191 None:intergenic 50.0%
TGATCACTCTACTGGCAGTC+TGG + Chr6:25244485-25244504 None:intergenic 50.0%
TGATTGTGGGAACACCAGGA+CGG - Chr6:25245404-25245423 MsG0680031717.01.T01:intron 50.0%
TTCTTCCCCATCAAAGCAGC+GGG + Chr6:25245118-25245137 None:intergenic 50.0%
! AAGACATCCAGCACTGGATG+AGG + Chr6:25244840-25244859 None:intergenic 50.0%
! ACCACAAGGGTTTTCTCTGG+AGG - Chr6:25246168-25246187 MsG0680031717.01.T01:CDS 50.0%
! GAAGAGCACGCGAAATTGCT+CGG - Chr6:25245295-25245314 MsG0680031717.01.T01:intron 50.0%
! GCATCTAGAATGGGAGAGGA+AGG - Chr6:25246033-25246052 MsG0680031717.01.T01:intron 50.0%
! GGTTGTGGCTACTGGAGTTT+TGG - Chr6:25245921-25245940 MsG0680031717.01.T01:intron 50.0%
! GTGGACTGCATGCTTCAAAG+GGG - Chr6:25245493-25245512 MsG0680031717.01.T01:intron 50.0%
! TCCAGCACTGGATGAGGATT+TGG + Chr6:25244834-25244853 None:intergenic 50.0%
! TCGGCTGCAGGTAGCTTTTT+AGG + Chr6:25244869-25244888 None:intergenic 50.0%
!! AAGGGCATCTAGAATGGGAG+AGG - Chr6:25246029-25246048 MsG0680031717.01.T01:intron 50.0%
!! ACGGCCTTGTTTGGTCTGTT+AGG + Chr6:25245658-25245677 None:intergenic 50.0%
!! AGCTGTTTCACGGCCTTGTT+TGG + Chr6:25245667-25245686 None:intergenic 50.0%
!! ATATACGTCGGCTCGAGACT+CGG - Chr6:25245772-25245791 MsG0680031717.01.T01:intron 50.0%
!! GCTGCAGGTAGCTTTTTAGG+TGG + Chr6:25244866-25244885 None:intergenic 50.0%
!! GTTTTGGCCGATACAGGTTC+AGG - Chr6:25245145-25245164 MsG0680031717.01.T01:intron 50.0%
!!! GCAGGTAGCTTTTTAGGTGG+TGG + Chr6:25244863-25244882 None:intergenic 50.0%
!!! GCTACTGGAGTTTTGGGTAG+AGG - Chr6:25245928-25245947 MsG0680031717.01.T01:intron 50.0%
ACTCCAGTAGCCACAACCAC+CGG + Chr6:25245919-25245938 None:intergenic 55.0%
CCAGAGAGATGCTCTTCCTG+GGG - Chr6:25244703-25244722 MsG0680031717.01.T01:CDS 55.0%
GACCAGAGAGATGCTCTTCC+TGG - Chr6:25244701-25244720 MsG0680031717.01.T01:CDS 55.0%
GATAAGCTCCCGAGGTACAG+CGG + Chr6:25246136-25246155 None:intergenic 55.0%
TCAGGAACGCCGTCTTTAGG+AGG - Chr6:25244923-25244942 MsG0680031717.01.T01:intron 55.0%
TCTGCAGGAGTTTGTCAGGG+AGG + Chr6:25245042-25245061 None:intergenic 55.0%
TCTTCCCCATCAAAGCAGCG+GGG + Chr6:25245117-25245136 None:intergenic 55.0%
TGGTGGAAGACATCCAGCAC+TGG + Chr6:25244846-25244865 None:intergenic 55.0%
! AGCAATCCCCGCTGCTTTGA+TGG - Chr6:25245108-25245127 MsG0680031717.01.T01:intron 55.0%
! CAATCCCCGCTGCTTTGATG+GGG - Chr6:25245110-25245129 MsG0680031717.01.T01:intron 55.0%
! CACGGCTTTGTCACAACCTC+AGG - Chr6:25245543-25245562 MsG0680031717.01.T01:intron 55.0%
! GCAATCCCCGCTGCTTTGAT+GGG - Chr6:25245109-25245128 MsG0680031717.01.T01:intron 55.0%
! GCCGTCTTTAGGAGGTGATC+AGG - Chr6:25244931-25244950 MsG0680031717.01.T01:intron 55.0%
! TAAATGCACTCGTCGGCTGC+AGG + Chr6:25244881-25244900 None:intergenic 55.0%
!! GAAGGGTGTTGTTTCGGCAC+CGG - Chr6:25244994-25245013 MsG0680031717.01.T01:intron 55.0%
!! TATACGTCGGCTCGAGACTC+GGG - Chr6:25245773-25245792 MsG0680031717.01.T01:intron 55.0%
!! TGTGGTTGGTGGTGATGCCA+TGG - Chr6:25245345-25245364 MsG0680031717.01.T01:intron 55.0%
ACAATACACTGGGGCTCCCC+AGG + Chr6:25244722-25244741 None:intergenic 60.0%
CTCCAGTAGCCACAACCACC+GGG + Chr6:25245918-25245937 None:intergenic 60.0%
GAGACTCGGGGCAGATCTCT+TGG - Chr6:25245786-25245805 MsG0680031717.01.T01:intron 60.0%
TTCCCGGTGGTTGTGGCTAC+TGG - Chr6:25245913-25245932 MsG0680031717.01.T01:intron 60.0%
!! ATACGTCGGCTCGAGACTCG+GGG - Chr6:25245774-25245793 MsG0680031717.01.T01:intron 60.0%
!! CCCCAGGAAGAGCATCTCTC+TGG + Chr6:25244706-25244725 None:intergenic 60.0%
AAGCTCCCGAGGTACAGCGG+CGG + Chr6:25246133-25246152 None:intergenic 65.0%
TATGGTCGGCGAGTTCCCGG+TGG - Chr6:25245900-25245919 MsG0680031717.01.T01:intron 65.0%
CGGCGAGTTCCCGGTGGTTG+TGG - Chr6:25245906-25245925 MsG0680031717.01.T01:intron 70.0%
! CGGGTGCCGCCGCTGTACCT+CGG - Chr6:25246124-25246143 MsG0680031717.01.T01:intron 75.0%
! GGGTGCCGCCGCTGTACCTC+GGG - Chr6:25246125-25246144 MsG0680031717.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 25243235 25246221 25243235 ID=MsG0680031717.01;Name=MsG0680031717.01
Chr6 mRNA 25243235 25246221 25243235 ID=MsG0680031717.01.T01;Parent=MsG0680031717.01;Name=MsG0680031717.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|538
Chr6 exon 25246158 25246221 25246158 ID=MsG0680031717.01.T01:exon:15534;Parent=MsG0680031717.01.T01
Chr6 exon 25243235 25244787 25243235 ID=MsG0680031717.01.T01:exon:15533;Parent=MsG0680031717.01.T01
Chr6 CDS 25246158 25246221 25246158 ID=MsG0680031717.01.T01:cds;Parent=MsG0680031717.01.T01
Chr6 CDS 25243235 25244787 25243235 ID=MsG0680031717.01.T01:cds;Parent=MsG0680031717.01.T01
Gene Sequence

>MsG0680031717.01.T01

ATGGTTGATGATTCCAACGATGACCCATCAAAGTTTCAATCGCTTTCAACCGATTCACCTAATGATGCAGGGAACGATGTCAAGGTGAGTTCTAAGGACCAGAGAGATGCTCTTCCTGGGGAGCCCCAGTGTATTGTATGTGGCCGATACGGTGAATATATATGTGATGAAACTGATGATGATGTTTGTAGTTTGGAATGTAAACAATCGGTACTATCTCGAATTTCCAAATCCTCATCCAGTGCTGGATGTCTTCCACCACCTAAAAAGCTACCTGCAGCCGACGAGTGCATTTATGTTAGAGATACTGACAACAAATCAGGAACGCCGTCTTTAGGAGGTGATCAGGTTCAGGCTGAATTGCTTAGAAAGAAACTCGACATTCATGTGAAGGGTGTTGTTTCGGCACCGGTTATGTCGTTTGCTTCATGTAACCTCCCTGACAAACTCCTGCAGAACATAGAAGCAGCTGGTTATGAGATGCCTACGCCTGTTCAAATGCAAGCAATCCCCGCTGCTTTGATGGGGAAGAACATGCTTGTTTTGGCCGATACAGGTTCAGGAAAGTCTGCTTCGTTTCTTATTCCAATAATATCTCGTTGTGCAAGTCATCGTTTGATGTATGTTTCGGATAACAAAAAGCCTCTAGCTATGGTGTTAACACCTACGAGAGAGCTCAGTATACAGGTTGAAGAGCACGCGAAATTGCTCGGAAAAGGTTTGCTGTTTAAAACTGCACTTGTGGTTGGTGGTGATGCCATGGCTAGACAACTTTACCGCATTCAACAAGGAGTTGAATTGATTGTGGGAACACCAGGACGGCTTGTTGATCTTTTAACAAAAAATGAGATTGATTTAGATGACGTGACGACTTTTGTTGTCGATGAAGTGGACTGCATGCTTCAAAGGGGTTTTCGAGATCAGGTATTGCAGATATTCACGGCTTTGTCACAACCTCAGGTCTTAATGTATTCCGCAACAACGTCCGATTCTTTAGAGAAGATGGCAAACTCTATGGCAAAAGATACAGTGGTTATAACCGTTGGTGAACCTAACAGACCAAACAAGGCCGTGAAACAGCTAGCAATTTGGGTCGAGTCGAAACAAAAGAAGCAGAAACTGTTTGATATTTTAAAAAGCCAGAAACATTTTAAGCCACCTGTTGTAATATACGTCGGCTCGAGACTCGGGGCAGATCTCTTGGCAAACGCAATTACAGTTTCAACAGGAGTTAAAGCTGTTTCAATTCACGGCGAGAAATCTATGACCGAAAGGAGAGAAATAATGCAGTCATTTATGGTCGGCGAGTTCCCGGTGGTTGTGGCTACTGGAGTTTTGGGTAGAGGGGTTGATCTATTAGGCGTAAGACAAGTTATTGTATTCGACATGCCTAATTCGATTAAGGAATATGTACATCAAATAGGAAGGGCATCTAGAATGGGAGAGGAAGGTCAAGCCTTAGTTTTTGTAAACGATGAGAATAAGAATGTTTTTGCAGAGTTAGTCGATGTATTAAAGTCGGGTGCCGCCGCTGTACCTCGGGAGCTTATCAATTCACAGTATACCACAAGGGTTTTCTCTGGAGGAAAGGGTTCAAAGAAAAGAAAATATAGATGA
Protein sequence

>MsG0680031717.01.T01

MVDDSNDDPSKFQSLSTDSPNDAGNDVKVSSKDQRDALPGEPQCIVCGRYGEYICDETDDDVCSLECKQSVLSRISKSSSSAGCLPPPKKLPAADECIYVRDTDNKSGTPSLGGDQVQAELLRKKLDIHVKGVVSAPVMSFASCNLPDKLLQNIEAAGYEMPTPVQMQAIPAALMGKNMLVLADTGSGKSASFLIPIISRCASHRLMYVSDNKKPLAMVLTPTRELSIQVEEHAKLLGKGLLFKTALVVGGDAMARQLYRIQQGVELIVGTPGRLVDLLTKNEIDLDDVTTFVVDEVDCMLQRGFRDQVLQIFTALSQPQVLMYSATTSDSLEKMANSMAKDTVVITVGEPNRPNKAVKQLAIWVESKQKKQKLFDILKSQKHFKPPVVIYVGSRLGADLLANAITVSTGVKAVSIHGEKSMTERREIMQSFMVGEFPVVVATGVLGRGVDLLGVRQVIVFDMPNSIKEYVHQIGRASRMGEEGQALVFVNDENKNVFAELVDVLKSGAAAVPRELINSQYTTRVFSGGKGSKKRKYR*