Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033723.01.T01 | XP_013443362.2 | 81.855 | 496 | 90 | 0 | 1 | 496 | 55 | 550 | 0 | 843 |
| MsG0680033723.01.T01 | XP_013443362.2 | 28.086 | 324 | 196 | 2 | 2 | 290 | 233 | 554 | 4.98E-35 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033723.01.T01 | A0A396HGV9 | 81.452 | 496 | 92 | 0 | 1 | 496 | 55 | 550 | 0.0 | 842 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033723.01.T01 | MTR_0019s0210 | 81.452 | 496 | 92 | 0 | 1 | 496 | 55 | 550 | 0.0 | 841 |
| MsG0680033723.01.T01 | MTR_0019s0210 | 28.086 | 324 | 196 | 2 | 2 | 290 | 233 | 554 | 8.01e-39 | 149 |
| MsG0680033723.01.T01 | MTR_3g034990 | 80.606 | 495 | 96 | 0 | 2 | 496 | 61 | 555 | 0.0 | 822 |
| MsG0680033723.01.T01 | MTR_7g012890 | 77.621 | 496 | 111 | 0 | 1 | 496 | 51 | 546 | 0.0 | 800 |
| MsG0680033723.01.T01 | MTR_0014s0250 | 76.210 | 496 | 117 | 1 | 1 | 496 | 51 | 545 | 0.0 | 780 |
| MsG0680033723.01.T01 | MTR_0014s0250 | 29.688 | 192 | 134 | 1 | 305 | 495 | 38 | 229 | 1.16e-17 | 86.3 |
| MsG0680033723.01.T01 | MTR_0014s0180 | 75.456 | 493 | 119 | 1 | 4 | 496 | 58 | 548 | 0.0 | 776 |
| MsG0680033723.01.T01 | MTR_0014s0180 | 29.558 | 362 | 220 | 1 | 164 | 490 | 41 | 402 | 3.49e-48 | 174 |
| MsG0680033723.01.T01 | MTR_0308s0020 | 73.790 | 496 | 130 | 0 | 1 | 496 | 46 | 541 | 0.0 | 771 |
| MsG0680033723.01.T01 | MTR_0308s0020 | 31.088 | 193 | 131 | 1 | 305 | 495 | 33 | 225 | 2.56e-20 | 94.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033723.01.T01 | AT1G62910 | 38.783 | 526 | 285 | 2 | 2 | 492 | 78 | 601 | 5.59e-128 | 386 |
| MsG0680033723.01.T01 | AT1G62910 | 31.307 | 329 | 226 | 0 | 165 | 493 | 64 | 392 | 4.36e-56 | 197 |
| MsG0680033723.01.T01 | AT1G62910 | 34.007 | 297 | 196 | 0 | 199 | 495 | 63 | 359 | 7.03e-46 | 169 |
| MsG0680033723.01.T01 | AT1G62910 | 27.919 | 197 | 142 | 0 | 294 | 490 | 53 | 249 | 1.04e-19 | 92.8 |
| MsG0680033723.01.T01 | AT1G12620 | 36.260 | 524 | 297 | 2 | 2 | 490 | 67 | 588 | 3.03e-126 | 381 |
| MsG0680033723.01.T01 | AT1G12620 | 32.190 | 379 | 257 | 0 | 115 | 493 | 73 | 451 | 3.83e-61 | 211 |
| MsG0680033723.01.T01 | AT1G12620 | 29.003 | 331 | 229 | 2 | 167 | 494 | 55 | 382 | 3.26e-44 | 165 |
| MsG0680033723.01.T01 | AT1G12620 | 26.842 | 190 | 139 | 0 | 306 | 495 | 54 | 243 | 1.94e-19 | 92.0 |
| MsG0680033723.01.T01 | AT1G62930 | 36.882 | 526 | 295 | 2 | 2 | 492 | 75 | 598 | 4.26e-125 | 379 |
| MsG0680033723.01.T01 | AT1G62930 | 28.741 | 421 | 300 | 0 | 63 | 483 | 64 | 484 | 2.55e-58 | 204 |
Find 88 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGTTGAGAAAGATTGAT+GGG | 0.583920 | 6:-72284456 | MsG0680033723.01.T01:CDS |
| AAGGAGAATCTCCTATGCTT+GGG | 0.420516 | 6:-72283849 | MsG0680033723.01.T01:CDS |
| AATAGTGTTTAATACATGTT+TGG | 0.380257 | 6:+72284042 | None:intergenic |
| AATCTTGGCCAACATAGAAA+AGG | 0.511130 | 6:+72284672 | None:intergenic |
| AATCTTGTTAAATTCAATGA+TGG | 0.442748 | 6:+72284843 | None:intergenic |
| ACAACTCAAGAATGCACGAG+AGG | 0.699111 | 6:-72283636 | MsG0680033723.01.T01:CDS |
| ACATACAATATACTTATTGA+TGG | 0.445423 | 6:-72283674 | MsG0680033723.01.T01:CDS |
| ACATACAATTCTCTCATTGA+TGG | 0.380904 | 6:-72283884 | MsG0680033723.01.T01:CDS |
| ACATATAATATTATGATCAA+TGG | 0.467026 | 6:-72283569 | MsG0680033723.01.T01:CDS |
| ACCTACAGTTCATTAATGGA+TGG | 0.573928 | 6:-72284094 | MsG0680033723.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATACAATTCTCTCATTGA+TGG | - | Chr6:72284366-72284385 | MsG0680033723.01.T01:CDS | 30.0% | |
| ATAAAAAAGGGAAATAGCAG+TGG | + | Chr6:72283470-72283489 | None:intergenic | 30.0% | |
| ATAACCCATCTTAAGAATCT+TGG | + | Chr6:72283596-72283615 | None:intergenic | 30.0% | |
| ATATTGTATGTGCACATATC+TGG | + | Chr6:72284567-72284586 | None:intergenic | 30.0% | |
| ATGTATAGCACAATCATTGA+TGG | - | Chr6:72283841-72283860 | MsG0680033723.01.T01:CDS | 30.0% | |
| CAAAATTCATTTCACCAAGA+TGG | + | Chr6:72283558-72283577 | None:intergenic | 30.0% | |
| CAAATGTTGAGAAAGATTGA+TGG | - | Chr6:72283793-72283812 | MsG0680033723.01.T01:CDS | 30.0% | |
| CCTAGTTAACGAAGTTATTA+AGG | - | Chr6:72284185-72284204 | MsG0680033723.01.T01:CDS | 30.0% | |
| CCTTAATAACTTCGTTAACT+AGG | + | Chr6:72284188-72284207 | None:intergenic | 30.0% | |
| GAATTGTATGTTACCGTATT+AGG | + | Chr6:72284357-72284376 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 72283391 | 72284881 | 72283391 | ID=MsG0680033723.01;Name=MsG0680033723.01 |
| Chr6 | mRNA | 72283391 | 72284881 | 72283391 | ID=MsG0680033723.01.T01;Parent=MsG0680033723.01;Name=MsG0680033723.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|496 |
| Chr6 | exon | 72283391 | 72284881 | 72283391 | ID=MsG0680033723.01.T01:exon:4230;Parent=MsG0680033723.01.T01 |
| Chr6 | CDS | 72283391 | 72284881 | 72283391 | ID=MsG0680033723.01.T01:cds;Parent=MsG0680033723.01.T01 |
| Gene Sequence |
>MsG0680033723.01.T01 ATGCGTCATACTCCATCCATCATTGAATTTAACAAGATTTTAGGTTCCCTTGTTAAGTCCAACAATAACCACTACCCCACTGCTATTTCCCTTTTTTATCAATTGGAACTCAATCGAGTTACGCCTGATATTGTTACTTTCAACACTGTCATTAATTGTTATTGCCATCTTGGTGAAATGAATTTTGCCTTTTCTATGTTGGCCAAGATTCTTAAGATGGGTTATCACCCCGATACCATTACCTTGACTACACTTATCAAAGGTTTGTGTCTTGATGGTAAGGTCAAGGAAGCTCTTCACTTTCATGACCATGTGCTTGCACTTGGATTTCACTTCGACCAAGTTACCTATGGGACCTTGATCAATGGATTGTGTAAAATAGGGGAAACAAGAGCTGCTTTGCAAATGTTGAGAAAGATTGATGGGAAATTGGTCATGATCAATGTGGTAATGTATAGCACAATCATTGATGGTTTGTGTAAAGATAAACATGTATCTGATGCCTATGAATTATATTCTGAAATGACTGCAAAGAAAATTTATCCTGATGTTGTCACTTTCAATGCTCTAATTTATGGATTTTGCATTATTGGTCAATTGAAAGACGCCTTTGGTTTGTTCCATGAGATGGTATTAGAAAACAACAACCCGGATATTTATACTTTTAGCATATTGGTTGATGCTCTATGTAAGGAAGGAAAGATGAAAGAAGCTAAAAGTGTGATAGCTGTGATGATGAAAAAAGGTGTGATACCAGATGTTGTTACCTACAGTTCATTAATGGATGGATATTGCCTAGTTAACGAAGTTATTAAGGCCAAACATGTATTAAACACTATTTCTCAAATGGGAGTGGCTCATGATGCTCATAGTTATAGTATCATGATTAATGGATTTTGTAAGATTAAAATGGTTGATAAAGCCTTGAGTCTCTTCAATGAAATGCGTTGCAGAGGTATTACTCCTAATACGGTAACATACAATTCTCTCATTGATGGTTTGTGCAAATCAAGGAGAATCTCCTATGCTTGGGGACTTGTTGATGAGATGCATGATAATGGTCAACCAGCCGATATATTCACTTACAATTCTTTAATAGATGCTTTATGCAAAAACCAACATGTTGACAAGGCTATTGCATTAGTTAAGAAAATTAAAGAACATGGCATTCAACCAGATATGTGCACATACAATATACTTATTGATGGACTATGCAAAGGAGGACAACTCAAGAATGCACGAGAGGTTTTTCAGGATCTTTTGCTTAAGGGTTATAGTCTAGATATTCGAACATATAATATTATGATCAATGGACTTTGTAAAGAGGGTTTGATTGATGAAGTGCAAGCCTTGTTGTCAAAAATGGAGGACAATGGTATCATTCCTAATGCCGTAACTTATGAAACTCTTATCCTTGCTCTCTTTCATAAAGATGAGAATGAAAAGGCGGAGAAACTTCTTCGTGAAATGATTTCTAGAGCTCTACTTTAA |
| Protein sequence |
>MsG0680033723.01.T01 MRHTPSIIEFNKILGSLVKSNNNHYPTAISLFYQLELNRVTPDIVTFNTVINCYCHLGEMNFAFSMLAKILKMGYHPDTITLTTLIKGLCLDGKVKEALHFHDHVLALGFHFDQVTYGTLINGLCKIGETRAALQMLRKIDGKLVMINVVMYSTIIDGLCKDKHVSDAYELYSEMTAKKIYPDVVTFNALIYGFCIIGQLKDAFGLFHEMVLENNNPDIYTFSILVDALCKEGKMKEAKSVIAVMMKKGVIPDVVTYSSLMDGYCLVNEVIKAKHVLNTISQMGVAHDAHSYSIMINGFCKIKMVDKALSLFNEMRCRGITPNTVTYNSLIDGLCKSRRISYAWGLVDEMHDNGQPADIFTYNSLIDALCKNQHVDKAIALVKKIKEHGIQPDMCTYNILIDGLCKGGQLKNAREVFQDLLLKGYSLDIRTYNIMINGLCKEGLIDEVQALLSKMEDNGIIPNAVTYETLILALFHKDENEKAEKLLREMISRALL* |
