AlfalfaGEDB | MsG0180001046.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001046.01

KEGG: -
GO: GO:0005515
PFAM: PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001046.01.T01	XP_013466438.1	86.895	496	65	1	496	56	551	0	893
MsG0180001046.01.T01	XP_013466438.1	30.124	322	225	1	322	231	552	5.29E-47	182
MsG0180001046.01.T01	XP_013466438.1	28.086	324	233	173	496	53	376	2.43E-39	161

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001046.01.T01	Q9LQ16	38.418	531	292	1	1	496	74	604	4.67E-129	391

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001046.01.T01	A0A072VEP1	86.895	496	65	0	1	496	56	551	0.0	893

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0180001046.01	PPI
MsG0080048925.01	MsG0180001046.01	PPI
MsG0180001046.01	MsG0580029982.01	PPI
MsG0180001046.01	MsG0680034466.01	PPI
MsG0180001046.01	MsG0680035211.01	PPI
MsG0180001046.01	MsG0680033483.01	PPI
MsG0180001046.01	MsG0680035220.01	PPI
MsG0180001046.01	MsG0680033171.01	PPI
MsG0180001046.01	MsG0680030695.01	PPI
MsG0180001046.01	MsG0680034378.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001046.01.T01	MTR_1g029490	86.895	496	65	0	1	496	56	551	0.0	893
MsG0180001046.01.T01	MTR_1g029490	30.124	322	225	0	1	322	231	552	6.40e-51	182
MsG0180001046.01.T01	MTR_1g029490	28.086	324	233	0	173	496	53	376	2.94e-43	161
MsG0180001046.01.T01	MTR_6g079310	75.000	496	124	0	1	496	5	500	0.0	771
MsG0180001046.01.T01	MTR_6g079310	29.841	315	221	0	8	322	187	501	3.72e-48	174
MsG0180001046.01.T01	MTR_6g079310	27.298	359	226	1	172	495	1	359	1.12e-45	167
MsG0180001046.01.T01	MTR_6g078160	74.395	496	127	0	1	496	47	542	0.0	759
MsG0180001046.01.T01	MTR_6g078160	30.794	315	218	0	8	322	229	543	1.29e-47	173
MsG0180001046.01.T01	MTR_6g079920	71.774	496	140	0	1	496	76	571	0.0	746
MsG0180001046.01.T01	MTR_6g079920	26.494	385	283	0	112	496	47	431	5.00e-46	169

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001046.01.T01	AT1G62910	38.418	531	292	1	1	496	74	604	4.76e-130	391
MsG0180001046.01.T01	AT1G62910	30.874	366	218	1	166	496	64	429	6.04e-57	200
MsG0180001046.01.T01	AT1G62910	25.214	468	335	3	5	472	23	475	1.95e-55	196
MsG0180001046.01.T01	AT1G62910	26.764	411	300	1	1	411	214	623	1.95e-55	196
MsG0180001046.01.T01	AT1G62910	27.507	349	239	2	148	496	25	359	1.32e-39	152
MsG0180001046.01.T01	AT1G62670	37.288	531	298	2	1	496	72	602	2.79e-128	387
MsG0180001046.01.T01	AT1G62670	26.536	407	299	0	66	472	67	473	3.56e-56	198
MsG0180001046.01.T01	AT1G62670	29.676	401	212	4	166	496	62	462	4.44e-55	195
MsG0180001046.01.T01	AT1G62670	28.620	297	212	0	200	496	61	357	1.56e-34	137
MsG0180001046.01.T01	AT1G62670	31.321	265	176	2	233	494	59	320	7.55e-32	129

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Find 88 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATGAAAATGATATGG+CGG	0.592436	1:+15192181	MsG0180001046.01.T01:CDS
AAATGGAAGATGGGTGGGAT+AGG	0.506722	1:-15190766	None:intergenic
AACAGCAGTGGTGTAATGGT+TGG	0.658727	1:-15190818	None:intergenic
AACCTAAAATCATTCCAAAA+TGG	0.351432	1:-15190783	None:intergenic
AACTCGTCTCAGCAATTCCA+AGG	0.594286	1:-15191151	None:intergenic
AAGGCTTAAGGATGCACAAG+AGG	0.613910	1:+15191977	MsG0180001046.01.T01:CDS
AATCATTCCAAAATGGAAGA+TGG	0.441559	1:-15190776	None:intergenic
AATCTTAGCAAATACGGAAA+AGG	0.484126	1:-15190941	None:intergenic
AATTACATACAATTCTATAT+TGG	0.320386	1:+15191830	MsG0180001046.01.T01:CDS
AATTGCTGAGACGAGTTGAT+GGG	0.613083	1:+15191157	MsG0180001046.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCATTCCAAAATGGAAGA+TGG	-	Chr1:15190779-15190798	None:intergenic	30.0%
AATCTTAGCAAATACGGAAA+AGG	-	Chr1:15190944-15190963	None:intergenic	30.0%
ATAAATCTGGTTGAATACCT+TGG	-	Chr1:15191917-15191936	None:intergenic	30.0%
ATCATTCCAAAATGGAAGAT+GGG	-	Chr1:15190778-15190797	None:intergenic	30.0%
ATGTATAACACGATCATTGA+TGG	+	Chr1:15191204-15191223	MsG0180001046.01.T01:CDS	30.0%
ATTAAAGGTCTCTGTCTTAA+AGG	+	Chr1:15191009-15191028	MsG0180001046.01.T01:CDS	30.0%
CGGTTTGATATTGTTAGTTA+TGG	+	Chr1:15191084-15191103	MsG0180001046.01.T01:CDS	30.0%
CTGTCAAAAATGAAAGACAA+TGG	+	Chr1:15192107-15192126	MsG0180001046.01.T01:CDS	30.0%
GAACTATAAGTTATCGTATC+AGG	-	Chr1:15191720-15191739	None:intergenic	30.0%
GTAGTTAAAGTTATAGCATC+TGG	-	Chr1:15190985-15191004	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	15190751	15192241	15190751	ID=MsG0180001046.01;Name=MsG0180001046.01
Chr1	mRNA	15190751	15192241	15190751	ID=MsG0180001046.01.T01;Parent=MsG0180001046.01;Name=MsG0180001046.01.T01;_AED=0.29;_eAED=0.29;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|496
Chr1	exon	15190751	15192241	15190751	ID=MsG0180001046.01.T01:exon:30788;Parent=MsG0180001046.01.T01
Chr1	CDS	15190751	15192241	15190751	ID=MsG0180001046.01.T01:cds;Parent=MsG0180001046.01.T01

Gene Sequence

>MsG0180001046.01.T01

ATGCTTCATCAAAATCCTATCCCACCCATCTTCCATTTTGGAATGATTTTAGGTTCCCTTGTCAAGGCCAACCATTACACCACTGCTGTTTCACTTCATCGAAAAATGGAATTAAACGGAGTTGCATCAAACTTAATCACTATGAGCATCTTGATCAATTGTTTCTGTCATTTGGGTCAAAACTCTTTTTCCTTTTCCGTATTTGCTAAGATTCTTAAGATGGGTTACCAGCCAGATGCTATAACTTTAACTACACTCATTAAAGGTCTCTGTCTTAAAGGTGAGGTCCGTAAAGCATTGCACTTTCACGACAAGGCGGTAGCGCATGGATTTCGGTTTGATATTGTTAGTTATGGAACCTTGGTTAATGGATTATGTAAAGTTGGAGAAACAAGAGCTGCCTTGGAATTGCTGAGACGAGTTGATGGGAAACTGGTCCAGCCTAATGTGGTAATGTATAACACGATCATTGATGGTATGTGCACAGATAAACTTGTTAATGATGCATTTGATTTATATTCTGAAATGATTGCTAAGAGAATTTCTCCTGATGTTGTCACTTATAGTAGTTTGATTAGTGGCTTTTGCATTGTGGGTCAATTGAAAGATGCAATTGATTTGTTACATAAAATGATATTGGAAAACATTAACCCAAATGTTTATACCATGAGTATATTGGTTGATGCTTTTTGTAAGGAAGGAAAATCGAAAGAAGCTAAAGCAGTGGTTGCCGTTTTGATGAAAAAAGGCGTAAAACCTAATGTTGTTACTTATAGTTCTTTACTAGATGGATATTGTCTTGTTAAACAAGTGAACAAGGCCGAGGTTATATTCAACACTATGGCCCAAAGTGGAGTGATTGCTGATGTTCAAAGTTATAGCATCATGATTAATGGATTTTGTAAGATTAATATGGTGGATGAAGCTATCAATCTCTTCAAAGAAATGCAACGCAGACAACTCATTCCTGATACGATAACTTATAGTTCTCTTATTGATGGCTTGGGCAAATCAGGGAGAATCTCATATGCTTGGCAACTTGTAGGTGAGATGCATGATAGAGGTCAACAACCTAATATAATTACATACAATTCTATATTGGATGCTTTATTGAAAAATCGCCATGTTGACGAAGCAATCGCATTGTTAAGAAAATTTAAAAACCAAGGTATTCAACCAGATTTATGTACATATACTATTCTTATGAAGGGACTATGTCTGAATGGAAGGCTTAAGGATGCACAAGAGGTTTTTCAAGTTCTTCTAATGAAAGGCTACAATCTAAATGTGTATACGTATACTGTTATGATCCAAGGATTCTGTTACAAGGGCTTATTTGATGAAGCGCTGGCTTTGCTGTCAAAAATGAAAGACAATGGCTGCATGCCAAATGCTAAAACTTATGAAATAATTATTCTTTCTTTGTTAGAAAAAGATGAAAATGATATGGCGGAAAAACTTCTTCGTGAGATGATTACGAGAGGCCTATAG

Protein sequence

>MsG0180001046.01.T01

MLHQNPIPPIFHFGMILGSLVKANHYTTAVSLHRKMELNGVASNLITMSILINCFCHLGQNSFSFSVFAKILKMGYQPDAITLTTLIKGLCLKGEVRKALHFHDKAVAHGFRFDIVSYGTLVNGLCKVGETRAALELLRRVDGKLVQPNVVMYNTIIDGMCTDKLVNDAFDLYSEMIAKRISPDVVTYSSLISGFCIVGQLKDAIDLLHKMILENINPNVYTMSILVDAFCKEGKSKEAKAVVAVLMKKGVKPNVVTYSSLLDGYCLVKQVNKAEVIFNTMAQSGVIADVQSYSIMINGFCKINMVDEAINLFKEMQRRQLIPDTITYSSLIDGLGKSGRISYAWQLVGEMHDRGQQPNIITYNSILDALLKNRHVDEAIALLRKFKNQGIQPDLCTYTILMKGLCLNGRLKDAQEVFQVLLMKGYNLNVYTYTVMIQGFCYKGLFDEALALLSKMKDNGCMPNAKTYEIIILSLLEKDENDMAEKLLREMITRGL*