Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033770.01.T03 | MCI12079.1 | 61.538 | 65 | 25 | 0 | 1 | 65 | 91 | 155 | 2.07E-17 | 81.3 |
| MsG0680033770.01.T02 | PNX81800.1 | 76.923 | 52 | 12 | 0 | 1 | 52 | 29 | 80 | 4.98E-18 | 84.3 |
| MsG0680033770.01.T01 | MCI12079.1 | 77.5 | 80 | 14 | 1 | 1 | 76 | 1 | 80 | 6.14E-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033770.01.T01 | A0A392PKP9 | 77.500 | 80 | 14 | 1 | 1 | 76 | 1 | 80 | 2.93e-34 | 125 |
| MsG0680033770.01.T02 | A0A2K3LTD3 | 76.923 | 52 | 12 | 0 | 1 | 52 | 29 | 80 | 2.38e-18 | 84.3 |
| MsG0680033770.01.T03 | A0A392PKP9 | 61.538 | 65 | 25 | 0 | 1 | 65 | 91 | 155 | 9.86e-18 | 81.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048874.01 | MsG0680033770.01 | 0.803402 | 3.545745e-49 | 1.780222e-46 |
| MsG0680033770.01 | MsG0780038386.01 | 0.825449 | 4.660389e-54 | 4.248820e-51 |
| MsG0680030447.01 | MsG0680033770.01 | 0.802908 | 4.488276e-49 | 2.225075e-46 |
| MsG0280009514.01 | MsG0680033770.01 | 0.807800 | 4.233871e-50 | 2.380564e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033770.01.T03 | MTR_7g055630 | 52.381 | 63 | 30 | 0 | 1 | 63 | 122 | 184 | 2.16e-15 | 66.6 |
| MsG0680033770.01.T03 | MTR_8g019260 | 56.604 | 53 | 23 | 0 | 1 | 53 | 116 | 168 | 5.35e-14 | 62.8 |
| MsG0680033770.01.T03 | MTR_8g019110 | 56.604 | 53 | 23 | 0 | 1 | 53 | 116 | 168 | 5.35e-14 | 62.8 |
| MsG0680033770.01.T03 | MTR_8g019280 | 49.231 | 65 | 31 | 1 | 1 | 63 | 86 | 150 | 5.60e-13 | 59.7 |
| MsG0680033770.01.T03 | MTR_8g019290 | 49.231 | 65 | 31 | 1 | 1 | 63 | 116 | 180 | 9.89e-13 | 59.7 |
| MsG0680033770.01.T03 | MTR_0470s0010 | 47.619 | 63 | 32 | 1 | 1 | 63 | 124 | 185 | 1.45e-11 | 56.6 |
| MsG0680033770.01.T03 | MTR_8g019020 | 47.619 | 63 | 32 | 1 | 1 | 63 | 124 | 185 | 1.45e-11 | 56.6 |
| MsG0680033770.01.T02 | MTR_7g055630 | 88.889 | 45 | 5 | 0 | 8 | 52 | 67 | 111 | 8.46e-21 | 82.4 |
| MsG0680033770.01.T02 | MTR_8g019280 | 84.444 | 45 | 7 | 0 | 8 | 52 | 31 | 75 | 2.31e-19 | 77.8 |
| MsG0680033770.01.T02 | MTR_8g019290 | 84.444 | 45 | 7 | 0 | 8 | 52 | 61 | 105 | 4.78e-19 | 77.8 |
| MsG0680033770.01.T02 | MTR_8g019260 | 84.444 | 45 | 7 | 0 | 8 | 52 | 61 | 105 | 5.44e-19 | 77.4 |
| MsG0680033770.01.T02 | MTR_8g019110 | 84.444 | 45 | 7 | 0 | 8 | 52 | 61 | 105 | 5.44e-19 | 77.4 |
| MsG0680033770.01.T02 | MTR_0470s0010 | 80.000 | 45 | 9 | 0 | 8 | 52 | 69 | 113 | 2.56e-17 | 73.2 |
| MsG0680033770.01.T02 | MTR_8g019020 | 80.000 | 45 | 9 | 0 | 8 | 52 | 69 | 113 | 2.56e-17 | 73.2 |
| MsG0680033770.01.T02 | MTR_0470s0020 | 69.565 | 46 | 14 | 0 | 5 | 50 | 111 | 156 | 9.74e-14 | 65.1 |
| MsG0680033770.01.T02 | MTR_8g019050 | 69.565 | 46 | 14 | 0 | 5 | 50 | 111 | 156 | 9.74e-14 | 65.1 |
| MsG0680033770.01.T02 | MTR_0514s0010 | 67.391 | 46 | 15 | 0 | 5 | 50 | 113 | 158 | 3.31e-13 | 63.5 |
| MsG0680033770.01.T02 | MTR_8g018980 | 67.391 | 46 | 15 | 0 | 5 | 50 | 111 | 156 | 7.47e-13 | 62.4 |
| MsG0680033770.01.T01 | MTR_7g055630 | 71.250 | 80 | 19 | 1 | 1 | 76 | 32 | 111 | 4.47e-33 | 114 |
| MsG0680033770.01.T01 | MTR_8g019290 | 75.000 | 76 | 18 | 1 | 1 | 76 | 31 | 105 | 1.68e-32 | 113 |
| MsG0680033770.01.T01 | MTR_8g019260 | 75.000 | 76 | 18 | 1 | 1 | 76 | 31 | 105 | 4.05e-32 | 112 |
| MsG0680033770.01.T01 | MTR_8g019110 | 75.000 | 76 | 18 | 1 | 1 | 76 | 31 | 105 | 4.05e-32 | 112 |
| MsG0680033770.01.T01 | MTR_8g019280 | 73.684 | 76 | 19 | 1 | 1 | 76 | 1 | 75 | 7.43e-31 | 108 |
| MsG0680033770.01.T01 | MTR_0470s0010 | 67.901 | 81 | 21 | 1 | 1 | 76 | 33 | 113 | 5.08e-29 | 104 |
| MsG0680033770.01.T01 | MTR_8g019020 | 67.901 | 81 | 21 | 1 | 1 | 76 | 33 | 113 | 5.08e-29 | 104 |
| MsG0680033770.01.T01 | MTR_0470s0020 | 45.833 | 96 | 30 | 2 | 1 | 74 | 61 | 156 | 6.08e-16 | 71.6 |
| MsG0680033770.01.T01 | MTR_8g019050 | 45.833 | 96 | 30 | 2 | 1 | 74 | 61 | 156 | 6.08e-16 | 71.6 |
| MsG0680033770.01.T01 | MTR_0514s0010 | 44.792 | 96 | 31 | 2 | 1 | 74 | 63 | 158 | 2.75e-15 | 70.1 |
| MsG0680033770.01.T01 | MTR_8g018980 | 44.792 | 96 | 31 | 2 | 1 | 74 | 61 | 156 | 3.48e-15 | 69.7 |
| MsG0680033770.01.T01 | MTR_8g019450 | 41.414 | 99 | 32 | 2 | 1 | 75 | 61 | 157 | 8.74e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCGTGTGGGTTTACAATA+TGG | 0.259755 | 6:-73115948 | MsG0680033770.01.T01:CDS |
| ATGTGTAGGAGAAGCGATTT+TGG | 0.299560 | 6:-73116075 | MsG0680033770.01.T01:CDS |
| AGAGAACTCCAAATGGAATT+TGG | 0.310603 | 6:+73115850 | None:intergenic |
| TCATCATTTGTAGCATCTAT+TGG | 0.323032 | 6:+73115732 | None:intergenic |
| GTTATTCTTGTGCTTGTTAG+TGG | 0.346393 | 6:-73115981 | MsG0680033770.01.T01:CDS |
| ATCTCTTACTTACATGTTTG+CGG | 0.373998 | 6:-73115887 | MsG0680033770.01.T01:CDS |
| TCCATTTGGAGTTCTCTTCC+TGG | 0.394499 | 6:-73115844 | MsG0680033770.01.T01:CDS |
| TGTAAACCCACACGGTTGAT+AGG | 0.404496 | 6:+73115954 | None:intergenic |
| CCATTTGGAGTTCTCTTCCT+GGG | 0.433001 | 6:-73115843 | MsG0680033770.01.T01:CDS |
| CCTGGTTCGCTTATTTGTGA+GGG | 0.445666 | 6:-73115758 | MsG0680033770.01.T03:CDS |
| TGGTCACACCAAATTCCATT+TGG | 0.470752 | 6:-73115858 | MsG0680033770.01.T01:CDS |
| AACACCACCAGGTGGATTAG+TGG | 0.490475 | 6:+73116154 | None:intergenic |
| TCCTGGTTCGCTTATTTGTG+AGG | 0.506075 | 6:-73115759 | MsG0680033770.01.T03:CDS |
| CCCAGGAAGAGAACTCCAAA+TGG | 0.525070 | 6:+73115843 | None:intergenic |
| TTACATGTTTGCGGCAAACA+TGG | 0.526105 | 6:-73115878 | MsG0680033770.01.T01:CDS |
| CCCTCACAAATAAGCGAACC+AGG | 0.528019 | 6:+73115758 | None:intergenic |
| TTTCTTGCGAGTCATACAAT+CGG | 0.543737 | 6:+73116106 | None:intergenic |
| CTGGTTCGCTTATTTGTGAG+GGG | 0.563398 | 6:-73115757 | MsG0680033770.01.T03:CDS |
| AAACATGTAAGTAAGAGATA+AGG | 0.575957 | 6:+73115891 | None:intergenic |
| TCACTTGCCACTAATCCACC+TGG | 0.578702 | 6:-73116161 | MsG0680033770.01.T01:CDS |
| CTAGCTTGAAGAACACCACC+AGG | 0.600294 | 6:+73116143 | None:intergenic |
| GGAATTCCTATCAACCGTGT+GGG | 0.611420 | 6:-73115960 | MsG0680033770.01.T01:CDS |
| TGGAATTCCTATCAACCGTG+TGG | 0.638571 | 6:-73115961 | MsG0680033770.01.T01:CDS |
| AGCCATATTGTAAACCCACA+CGG | 0.705841 | 6:+73115946 | None:intergenic |
| GCTTGAAGAACACCACCAGG+TGG | 0.706334 | 6:+73116146 | None:intergenic |
| CTTGCCACTAATCCACCTGG+TGG | 0.717955 | 6:-73116158 | MsG0680033770.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAAAATTAATATGTGT+AGG | - | Chr6:73115816-73115835 | MsG0680033770.01.T01:CDS | 15.0% |
| !! | ATATCAAAAATCTTAAATAG+TGG | + | Chr6:73115872-73115891 | None:intergenic | 15.0% |
| ! | AAACATGTAAGTAAGAGATA+AGG | + | Chr6:73116017-73116036 | None:intergenic | 25.0% |
| !!! | TGTTTTTGGTATTGCTATTA+TGG | - | Chr6:73116087-73116106 | MsG0680033770.01.T01:CDS | 25.0% |
| ATCTCTTACTTACATGTTTG+CGG | - | Chr6:73116018-73116037 | MsG0680033770.01.T01:CDS | 30.0% | |
| ! | ACAATATGGCTTTTATCAAC+AGG | - | Chr6:73115971-73115990 | MsG0680033770.01.T01:CDS | 30.0% |
| !!! | AATTGTTTTTACTTCTCTCC+TGG | - | Chr6:73116129-73116148 | MsG0680033770.01.T01:CDS | 30.0% |
| AGAGAACTCCAAATGGAATT+TGG | + | Chr6:73116058-73116077 | None:intergenic | 35.0% | |
| TTTCTTGCGAGTCATACAAT+CGG | + | Chr6:73115802-73115821 | None:intergenic | 35.0% | |
| ! | GTTATTCTTGTGCTTGTTAG+TGG | - | Chr6:73115924-73115943 | MsG0680033770.01.T01:CDS | 35.0% |
| AACCGTGTGGGTTTACAATA+TGG | - | Chr6:73115957-73115976 | MsG0680033770.01.T01:CDS | 40.0% | |
| AGCCATATTGTAAACCCACA+CGG | + | Chr6:73115962-73115981 | None:intergenic | 40.0% | |
| GCAATACCAAAAACATTCCC+AGG | + | Chr6:73116082-73116101 | None:intergenic | 40.0% | |
| TGGTCACACCAAATTCCATT+TGG | - | Chr6:73116047-73116066 | MsG0680033770.01.T01:CDS | 40.0% | |
| TTACATGTTTGCGGCAAACA+TGG | - | Chr6:73116027-73116046 | MsG0680033770.01.T01:CDS | 40.0% | |
| ! | ATGTGTAGGAGAAGCGATTT+TGG | - | Chr6:73115830-73115849 | MsG0680033770.01.T01:CDS | 40.0% |
| ! | TCTCTTCCTGGGAATGTTTT+TGG | - | Chr6:73116073-73116092 | MsG0680033770.01.T01:CDS | 40.0% |
| GGAATTCCTATCAACCGTGT+GGG | - | Chr6:73115945-73115964 | MsG0680033770.01.T01:CDS | 45.0% | |
| TGGAATTCCTATCAACCGTG+TGG | - | Chr6:73115944-73115963 | MsG0680033770.01.T01:CDS | 45.0% | |
| TGTAAACCCACACGGTTGAT+AGG | + | Chr6:73115954-73115973 | None:intergenic | 45.0% | |
| ! | CCATTTGGAGTTCTCTTCCT+GGG | - | Chr6:73116062-73116081 | MsG0680033770.01.T01:CDS | 45.0% |
| ! | CCTGGTTCGCTTATTTGTGA+GGG | - | Chr6:73116147-73116166 | MsG0680033770.01.T01:CDS | 45.0% |
| ! | CTGGTTCGCTTATTTGTGAG+GGG | - | Chr6:73116148-73116167 | MsG0680033770.01.T01:CDS | 45.0% |
| ! | TCCATTTGGAGTTCTCTTCC+TGG | - | Chr6:73116061-73116080 | MsG0680033770.01.T01:CDS | 45.0% |
| ! | TCCTGGTTCGCTTATTTGTG+AGG | - | Chr6:73116146-73116165 | MsG0680033770.01.T01:CDS | 45.0% |
| CCCAGGAAGAGAACTCCAAA+TGG | + | Chr6:73116065-73116084 | None:intergenic | 50.0% | |
| CCCTCACAAATAAGCGAACC+AGG | + | Chr6:73116150-73116169 | None:intergenic | 50.0% | |
| CTAGCTTGAAGAACACCACC+AGG | + | Chr6:73115765-73115784 | None:intergenic | 50.0% | |
| TCACTTGCCACTAATCCACC+TGG | - | Chr6:73115744-73115763 | MsG0680033770.01.T03:CDS | 50.0% | |
| !! | AACACCACCAGGTGGATTAG+TGG | + | Chr6:73115754-73115773 | None:intergenic | 50.0% |
| CTTGCCACTAATCCACCTGG+TGG | - | Chr6:73115747-73115766 | MsG0680033770.01.T03:CDS | 55.0% | |
| GCTTGAAGAACACCACCAGG+TGG | + | Chr6:73115762-73115781 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 73115735 | 73116192 | 73115735 | ID=MsG0680033770.01;Name=MsG0680033770.01 |
| Chr6 | mRNA | 73115803 | 73116192 | 73115803 | ID=MsG0680033770.01.T01;Parent=MsG0680033770.01;Name=MsG0680033770.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|0|1|0|129 |
| Chr6 | exon | 73115803 | 73116192 | 73115803 | ID=MsG0680033770.01.T01:exon:3847;Parent=MsG0680033770.01.T01 |
| Chr6 | CDS | 73115803 | 73116192 | 73115803 | ID=MsG0680033770.01.T01:cds;Parent=MsG0680033770.01.T01 |
| Chr6 | mRNA | 73115803 | 73116120 | 73115803 | ID=MsG0680033770.01.T02;Parent=MsG0680033770.01;Name=MsG0680033770.01.T02;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|0|1|0|105 |
| Chr6 | exon | 73115803 | 73116120 | 73115803 | ID=MsG0680033770.01.T02:exon:3848;Parent=MsG0680033770.01.T02 |
| Chr6 | CDS | 73115803 | 73116120 | 73115803 | ID=MsG0680033770.01.T02:cds;Parent=MsG0680033770.01.T02 |
| Chr6 | mRNA | 73115735 | 73115932 | 73115735 | ID=MsG0680033770.01.T03;Parent=MsG0680033770.01;Name=MsG0680033770.01.T03;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|0|1|0|65 |
| Chr6 | exon | 73115735 | 73115932 | 73115735 | ID=MsG0680033770.01.T03:exon:3849;Parent=MsG0680033770.01.T03 |
| Chr6 | CDS | 73115735 | 73115932 | 73115735 | ID=MsG0680033770.01.T03:cds;Parent=MsG0680033770.01.T03 |
| Gene Sequence |
| Protein sequence |
