Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034091.01.T01 | AES87058.2 | 51.818 | 110 | 41 | 4 | 3 | 111 | 1733 | 1831 | 4.36E-20 | 95.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034091.01.T01 | G7JSB9 | 51.818 | 110 | 41 | 4 | 3 | 111 | 1733 | 1831 | 2.08e-20 | 95.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680034091.01 | MsG0680034092.01 | 0.819600 | 1.066808e-52 | 8.246383e-50 |
| MsG0380015877.01 | MsG0680034091.01 | 0.810179 | 1.310892e-50 | 7.848014e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034091.01.T01 | MTR_4g020640 | 51.818 | 110 | 41 | 4 | 3 | 111 | 1733 | 1831 | 5.28e-24 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACTCAAATCACAATAAA+TGG | 0.256489 | 6:+80349734 | None:intergenic |
| CTTCTATGGTTGCAGGAACT+TGG | 0.335593 | 6:-80350116 | None:intergenic |
| TGTGAACGTTGAACTCTATT+TGG | 0.351765 | 6:-80349706 | MsG0680034091.01.T01:CDS |
| GTTGACATGTTTGCTTTAGT+TGG | 0.402973 | 6:-80349845 | MsG0680034091.01.T01:intron |
| CATAGCTAAAACAACTAGTA+AGG | 0.490428 | 6:+80349680 | None:intergenic |
| TTCTATGGTTGCAGGAACTT+GGG | 0.493296 | 6:-80350115 | None:intergenic |
| ACATGTATCTCAGTGTGTAT+TGG | 0.506796 | 6:-80349629 | MsG0680034091.01.T01:CDS |
| GCAAACATGTCAACCTCTTG+TGG | 0.544267 | 6:+80349854 | None:intergenic |
| CATACAGAACATGGTAGACA+AGG | 0.572772 | 6:-80350032 | MsG0680034091.01.T01:CDS |
| ATTGAAATCGAAGCCACAAG+AGG | 0.600831 | 6:-80349867 | MsG0680034091.01.T01:CDS |
| TTAGAGTGGTGATCGATAGA+CGG | 0.615410 | 6:-80350088 | MsG0680034091.01.T01:CDS |
| TCTCAGTGTGTATTGGACAT+TGG | 0.630806 | 6:-80349622 | MsG0680034091.01.T01:CDS |
| GGAACTTGGGTCTCTTAGAG+TGG | 0.645033 | 6:-80350102 | MsG0680034091.01.T01:CDS |
| TGTATTGGACATTGGAAGTG+TGG | 0.673513 | 6:-80349614 | MsG0680034091.01.T01:CDS |
| TCACGAGTACATACAGAACA+TGG | 0.676915 | 6:-80350041 | MsG0680034091.01.T01:CDS |
| ATACAGAACATGGTAGACAA+GGG | 0.707864 | 6:-80350031 | MsG0680034091.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATGAAAAAAGATGGACAT+AGG | - | Chr6:80349648-80349667 | MsG0680034091.01.T01:CDS | 25.0% |
| AGACGGAAAATGAAAAAAGA+TGG | - | Chr6:80349641-80349660 | MsG0680034091.01.T01:CDS | 30.0% | |
| CATAGCTAAAACAACTAGTA+AGG | + | Chr6:80350035-80350054 | None:intergenic | 30.0% | |
| TGCACTCAAATCACAATAAA+TGG | + | Chr6:80349981-80350000 | None:intergenic | 30.0% | |
| ACATGTATCTCAGTGTGTAT+TGG | - | Chr6:80350083-80350102 | MsG0680034091.01.T01:CDS | 35.0% | |
| ATACAGAACATGGTAGACAA+GGG | - | Chr6:80349681-80349700 | MsG0680034091.01.T01:CDS | 35.0% | |
| ATATCTCGTATCCAGAAACA+TGG | - | Chr6:80349776-80349795 | MsG0680034091.01.T01:intron | 35.0% | |
| GGATACGAGATATGATATGA+AGG | + | Chr6:80349769-80349788 | None:intergenic | 35.0% | |
| GTTGACATGTTTGCTTTAGT+TGG | - | Chr6:80349867-80349886 | MsG0680034091.01.T01:CDS | 35.0% | |
| TGTGAACGTTGAACTCTATT+TGG | - | Chr6:80350006-80350025 | MsG0680034091.01.T01:intron | 35.0% | |
| ! | GTTAGATGATGATGAGCATT+TGG | - | Chr6:80349913-80349932 | MsG0680034091.01.T01:intron | 35.0% |
| !!! | CTAGTTGTTTTAGCTATGCA+TGG | - | Chr6:80350037-80350056 | MsG0680034091.01.T01:CDS | 35.0% |
| ATAAGGTCACACCATGTTTC+TGG | + | Chr6:80349790-80349809 | None:intergenic | 40.0% | |
| ATTGAAATCGAAGCCACAAG+AGG | - | Chr6:80349845-80349864 | MsG0680034091.01.T01:intron | 40.0% | |
| CATACAGAACATGGTAGACA+AGG | - | Chr6:80349680-80349699 | MsG0680034091.01.T01:CDS | 40.0% | |
| CATCATCATCTAACTACTCC+TGG | + | Chr6:80349907-80349926 | None:intergenic | 40.0% | |
| GTGACCTTATGATAACCACA+GGG | - | Chr6:80349800-80349819 | MsG0680034091.01.T01:intron | 40.0% | |
| TCACGAGTACATACAGAACA+TGG | - | Chr6:80349671-80349690 | MsG0680034091.01.T01:CDS | 40.0% | |
| TCTCAGTGTGTATTGGACAT+TGG | - | Chr6:80350090-80350109 | MsG0680034091.01.T01:CDS | 40.0% | |
| TGACCTTATGATAACCACAG+GGG | - | Chr6:80349801-80349820 | MsG0680034091.01.T01:intron | 40.0% | |
| TGTATTGGACATTGGAAGTG+TGG | - | Chr6:80350098-80350117 | MsG0680034091.01.T01:CDS | 40.0% | |
| TGTGACCTTATGATAACCAC+AGG | - | Chr6:80349799-80349818 | MsG0680034091.01.T01:intron | 40.0% | |
| TTAGAGTGGTGATCGATAGA+CGG | - | Chr6:80349624-80349643 | MsG0680034091.01.T01:CDS | 40.0% | |
| ! | GATATGAAGGCTCTAGTGTT+TGG | + | Chr6:80349756-80349775 | None:intergenic | 40.0% |
| !!! | GTTTTAGCTATGCATGGTTG+TGG | - | Chr6:80350043-80350062 | MsG0680034091.01.T01:CDS | 40.0% |
| GCAAACATGTCAACCTCTTG+TGG | + | Chr6:80349861-80349880 | None:intergenic | 45.0% | |
| TGACCCCTGTGGTTATCATA+AGG | + | Chr6:80349807-80349826 | None:intergenic | 45.0% | |
| TTGGTTGCGTGATGTATACC+AGG | - | Chr6:80349886-80349905 | MsG0680034091.01.T01:CDS | 45.0% | |
| ! | GCATTTTTACTGCACGTTGC+AGG | - | Chr6:80349950-80349969 | MsG0680034091.01.T01:intron | 45.0% |
| GGAACTTGGGTCTCTTAGAG+TGG | - | Chr6:80349610-80349629 | MsG0680034091.01.T01:CDS | 50.0% | |
| !! | TGTACGAGTTTTGACCCCTG+TGG | + | Chr6:80349818-80349837 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 80349605 | 80350129 | 80349605 | ID=MsG0680034091.01;Name=MsG0680034091.01 |
| Chr6 | mRNA | 80349605 | 80350129 | 80349605 | ID=MsG0680034091.01.T01;Parent=MsG0680034091.01;Name=MsG0680034091.01.T01;_AED=0.48;_eAED=0.84;_QI=0|0|0|1|0.5|0.66|3|0|112 |
| Chr6 | exon | 80350017 | 80350129 | 80350017 | ID=MsG0680034091.01.T01:exon:8354;Parent=MsG0680034091.01.T01 |
| Chr6 | exon | 80349846 | 80349913 | 80349846 | ID=MsG0680034091.01.T01:exon:8355;Parent=MsG0680034091.01.T01 |
| Chr6 | exon | 80349605 | 80349762 | 80349605 | ID=MsG0680034091.01.T01:exon:8356;Parent=MsG0680034091.01.T01 |
| Chr6 | CDS | 80350017 | 80350129 | 80350017 | ID=MsG0680034091.01.T01:cds;Parent=MsG0680034091.01.T01 |
| Chr6 | CDS | 80349846 | 80349913 | 80349846 | ID=MsG0680034091.01.T01:cds;Parent=MsG0680034091.01.T01 |
| Chr6 | CDS | 80349605 | 80349762 | 80349605 | ID=MsG0680034091.01.T01:cds;Parent=MsG0680034091.01.T01 |
| Gene Sequence |
| Protein sequence |
