AlfalfaGEDB | MsG0380015877.01

M. sativa cultivar ZhongmuNo.1 / MsG0380015877.01

KEGG: K18875
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015877.01.T01	XP_003601315.3	92.308	65	5	0	1	65	423	487	2.88E-33	130

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015877.01.T01	Q9XF23	52.542	59	27	1	5	63	393	450	2.00E-13	66.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015877.01.T01	A0A396ITH9	92.308	65	5	0	1	65	423	487	1.38e-33	130

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048177.01	MsG0380015877.01	0.834447	2.981834e-56	3.539718e-53
MsG0080048178.01	MsG0380015877.01	0.832319	1.011583e-55	1.126531e-52
MsG0080048915.01	MsG0380015877.01	0.844419	7.647323e-59	1.233855e-55
MsG0180000154.01	MsG0380015877.01	0.821033	5.006875e-53	4.028141e-50
MsG0180000585.01	MsG0380015877.01	0.815939	7.149283e-52	4.997022e-49
MsG0180000822.01	MsG0380015877.01	0.826587	2.499767e-54	2.354158e-51
MsG0180001904.01	MsG0380015877.01	0.823808	1.134666e-53	9.873350e-51
MsG0180002894.01	MsG0380015877.01	0.821104	4.821117e-53	3.886149e-50
MsG0180002952.01	MsG0380015877.01	0.812957	3.264674e-51	2.104164e-48
MsG0180004060.01	MsG0380015877.01	0.812101	5.022358e-51	3.164306e-48
MsG0180004114.01	MsG0380015877.01	0.820746	5.826656e-53	4.649831e-50
MsG0180005172.01	MsG0380015877.01	0.814910	1.211085e-51	8.229994e-49
MsG0180005278.01	MsG0380015877.01	0.800260	1.566658e-48	7.261869e-46
MsG0180005279.01	MsG0380015877.01	0.823115	1.647597e-53	1.405840e-50
MsG0180005692.01	MsG0380015877.01	0.801062	1.075132e-48	5.086170e-46
MsG0180006257.01	MsG0380015877.01	0.808945	2.413108e-50	1.398211e-47
MsG0280010614.01	MsG0380015877.01	0.840381	8.997351e-58	1.279400e-54
MsG0280010648.01	MsG0380015877.01	0.804886	1.741997e-49	9.087153e-47
MsG0280010698.01	MsG0380015877.01	0.812032	5.198660e-51	3.269113e-48
MsG0280011024.01	MsG0380015877.01	0.843834	1.098124e-58	1.739643e-55
MsG0280011052.01	MsG0380015877.01	0.809757	1.615630e-50	9.567171e-48
MsG0280011163.01	MsG0380015877.01	0.802761	4.812628e-49	2.376998e-46
MsG0280011293.01	MsG0380015877.01	0.801735	7.826700e-49	3.765737e-46
MsG0280011334.01	MsG0380015877.01	0.808995	2.354369e-50	1.366024e-47
MsG0380011663.01	MsG0380015877.01	0.827275	1.711393e-54	1.644428e-51
MsG0380011754.01	MsG0380015877.01	0.811041	8.536796e-51	5.229468e-48
MsG0380012021.01	MsG0380015877.01	0.824348	8.472715e-54	7.485175e-51
MsG0380012033.01	MsG0380015877.01	0.845904	3.036181e-59	5.136929e-56
MsG0380013176.01	MsG0380015877.01	0.840721	7.333290e-58	1.053796e-54
MsG0380013436.01	MsG0380015877.01	0.833436	5.339726e-56	6.149145e-53
MsG0380014122.01	MsG0380015877.01	0.812477	4.156146e-51	2.645425e-48
MsG0380014135.01	MsG0380015877.01	0.809733	1.635644e-50	9.679182e-48
MsG0380014163.01	MsG0380015877.01	0.803226	3.857587e-49	1.927938e-46
MsG0380014573.01	MsG0380015877.01	0.847187	1.354881e-59	2.388979e-56
MsG0380015852.01	MsG0380015877.01	0.838093	3.529216e-57	4.677271e-54
MsG0380015877.01	MsG0380015878.01	0.895222	1.176059e-75	1.209052e-71
MsG0380015877.01	MsG0380015931.01	0.826020	3.411657e-54	3.161697e-51
MsG0380015877.01	MsG0380015986.01	0.824021	1.011481e-53	8.853049e-51
MsG0380015877.01	MsG0380016236.01	0.809331	1.995301e-50	1.168166e-47
MsG0380015877.01	MsG0380016909.01	0.814129	1.802765e-51	1.199532e-48
MsG0380015877.01	MsG0380017140.01	0.803635	3.172583e-49	1.602390e-46
MsG0380015877.01	MsG0380017940.01	0.844964	5.453674e-59	8.952016e-56
MsG0380015877.01	MsG0480018321.01	0.805701	1.175712e-49	6.262693e-47
MsG0380015877.01	MsG0480018640.01	0.808819	2.567552e-50	1.482739e-47
MsG0380015877.01	MsG0480019034.01	0.801357	9.354840e-49	4.458467e-46
MsG0380015877.01	MsG0480019329.01	0.806775	6.982598e-50	3.822739e-47
MsG0380015877.01	MsG0480019435.01	0.809269	2.056403e-50	1.201977e-47
MsG0380015877.01	MsG0480020445.01	0.800620	1.322950e-48	6.188610e-46
MsG0380015877.01	MsG0480020886.01	0.805095	1.574991e-49	8.259348e-47
MsG0380015877.01	MsG0480021021.01	0.823371	1.435942e-53	1.233933e-50
MsG0380015877.01	MsG0480021438.01	0.832245	1.055303e-55	1.172684e-52
MsG0380015877.01	MsG0480021441.01	0.823095	1.665281e-53	1.420126e-50
MsG0380015877.01	MsG0480021900.01	0.814852	1.247443e-51	8.464046e-49
MsG0380015877.01	MsG0480021927.01	0.809581	1.762974e-50	1.039096e-47
MsG0380015877.01	MsG0480022406.01	0.812401	4.318871e-51	2.743484e-48
MsG0380015877.01	MsG0480022451.01	0.818832	1.595457e-52	1.207338e-49
MsG0380015877.01	MsG0480022575.01	0.808393	3.166627e-50	1.808526e-47
MsG0380015877.01	MsG0480022597.01	0.807498	4.908459e-50	2.738288e-47
MsG0380015877.01	MsG0480022598.01	0.805755	1.145322e-49	6.109308e-47
MsG0380015877.01	MsG0480022862.01	0.813066	3.089405e-51	1.997116e-48
MsG0380015877.01	MsG0480023223.01	0.800363	1.492640e-48	6.937119e-46
MsG0380015877.01	MsG0480023224.01	0.825471	4.605066e-54	4.201099e-51
MsG0380015877.01	MsG0480023245.01	0.803300	3.722662e-49	1.864040e-46
MsG0380015877.01	MsG0480023248.01	0.804013	2.648107e-49	1.350577e-46
MsG0380015877.01	MsG0480023427.01	0.803541	3.317973e-49	1.671873e-46
MsG0380015877.01	MsG0480023898.01	0.835369	1.746238e-56	2.131132e-53
MsG0380015877.01	MsG0480023900.01	0.811255	7.671378e-51	4.725727e-48
MsG0380015877.01	MsG0480023903.01	0.852860	3.498649e-61	7.413238e-58
MsG0380015877.01	MsG0580024157.01	0.801925	7.153660e-49	3.458686e-46
MsG0380015877.01	MsG0580024205.01	0.839367	1.652855e-57	2.277637e-54
MsG0380015877.01	MsG0580024305.01	0.800522	1.385678e-48	6.465779e-46
MsG0380015877.01	MsG0580024306.01	0.804276	2.334479e-49	1.198653e-46
MsG0380015877.01	MsG0580024433.01	0.801512	8.695588e-49	4.160424e-46
MsG0380015877.01	MsG0580024682.01	0.815179	1.055244e-51	7.223529e-49
MsG0380015877.01	MsG0580024781.01	0.804977	1.667403e-49	8.717757e-47
MsG0380015877.01	MsG0580024826.01	0.817913	2.577329e-52	1.901488e-49
MsG0380015877.01	MsG0580024853.01	0.822120	2.807014e-53	2.328174e-50
MsG0380015877.01	MsG0580024856.01	0.808360	3.218768e-50	1.836726e-47
MsG0380015877.01	MsG0580024918.01	0.805517	1.284739e-49	6.810594e-47
MsG0380015877.01	MsG0580025008.01	0.835144	1.990322e-56	2.412377e-53
MsG0380015877.01	MsG0580025022.01	0.817910	2.580298e-52	1.903565e-49
MsG0380015877.01	MsG0580025206.01	0.808735	2.676770e-50	1.542308e-47
MsG0380015877.01	MsG0580027129.01	0.819177	1.332067e-52	1.017624e-49
MsG0380015877.01	MsG0580027617.01	0.812447	4.221220e-51	2.684706e-48
MsG0380015877.01	MsG0580027661.01	0.806823	6.823164e-50	3.740168e-47
MsG0380015877.01	MsG0580027773.01	0.812292	4.563497e-51	2.890247e-48
MsG0380015877.01	MsG0580027827.01	0.808943	2.415713e-50	1.399655e-47
MsG0380015877.01	MsG0580027832.01	0.815733	7.946855e-52	5.523827e-49
MsG0380015877.01	MsG0580027838.01	0.819548	1.096374e-52	8.462860e-50
MsG0380015877.01	MsG0580028529.01	0.810441	1.151153e-50	6.939296e-48
MsG0380015877.01	MsG0580028969.01	0.801425	9.058465e-49	4.324545e-46
MsG0380015877.01	MsG0580029101.01	0.814668	1.370463e-51	9.252624e-49
MsG0380015877.01	MsG0680030629.01	0.873246	1.705927e-67	7.352061e-64
MsG0380015877.01	MsG0680030685.01	0.815696	8.099675e-52	5.624025e-49
MsG0380015877.01	MsG0680030694.01	0.881011	3.421182e-70	1.971329e-66
MsG0380015877.01	MsG0680030695.01	0.860076	2.657406e-63	7.175308e-60
MsG0380015877.01	MsG0680030696.01	0.821418	4.080394e-53	3.317853e-50
MsG0380015877.01	MsG0680030995.01	0.814375	1.591072e-51	1.065785e-48
MsG0380015877.01	MsG0680031459.01	0.801894	7.258141e-49	3.506637e-46
MsG0380015877.01	MsG0680031719.01	0.819389	1.191902e-52	9.158805e-50
MsG0380015877.01	MsG0680034036.01	0.803360	3.618607e-49	1.814679e-46
MsG0380015877.01	MsG0680034091.01	0.810179	1.310892e-50	7.848014e-48
MsG0380015877.01	MsG0680034093.01	0.811454	6.946591e-51	4.301802e-48
MsG0380015877.01	MsG0680034753.01	0.818727	1.685522e-52	1.271761e-49
MsG0380015877.01	MsG0680034843.01	0.800227	1.590846e-48	7.367849e-46
MsG0380015877.01	MsG0680035444.01	0.807172	5.755637e-50	3.183682e-47
MsG0380015877.01	MsG0680035455.01	0.819279	1.262785e-52	9.674025e-50
MsG0380015877.01	MsG0680035460.01	0.857301	1.791571e-62	4.401208e-59
MsG0380015877.01	MsG0680035573.01	0.800557	1.362808e-48	6.364819e-46
MsG0380015877.01	MsG0680035656.01	0.854006	1.640272e-61	3.611794e-58
MsG0380015877.01	MsG0680035666.01	0.803187	3.928864e-49	1.961666e-46
MsG0380015877.01	MsG0680035716.01	0.807714	4.416510e-50	2.477668e-47
MsG0380015877.01	MsG0680035844.01	0.831386	1.718969e-55	1.862558e-52
MsG0380015877.01	MsG0780035958.01	0.837771	4.270378e-57	5.605042e-54
MsG0380015877.01	MsG0780037630.01	0.801759	7.738689e-49	3.725628e-46
MsG0380015877.01	MsG0780037859.01	0.827593	1.435809e-54	1.392256e-51
MsG0380015877.01	MsG0780038113.01	0.875418	3.129914e-68	1.460138e-64
MsG0380015877.01	MsG0780038386.01	0.846793	1.737212e-59	3.024750e-56
MsG0380015877.01	MsG0780038401.01	0.838097	3.521323e-57	4.667266e-54
MsG0380015877.01	MsG0780038597.01	0.804444	2.154304e-49	1.110897e-46
MsG0380015877.01	MsG0780038825.01	0.812392	4.338252e-51	2.755084e-48
MsG0380015877.01	MsG0780038829.01	0.814889	1.224200e-51	8.314424e-49
MsG0380015877.01	MsG0780038836.01	0.806766	7.012082e-50	3.838104e-47
MsG0380015877.01	MsG0780039252.01	0.832763	7.852398e-56	8.862136e-53
MsG0380015877.01	MsG0780039655.01	0.808875	2.498429e-50	1.444928e-47
MsG0380015877.01	MsG0780039875.01	0.815924	7.205018e-52	5.033816e-49
MsG0380015877.01	MsG0780040186.01	0.802300	5.989643e-49	2.923865e-46
MsG0380015877.01	MsG0780040516.01	0.837830	4.124385e-57	5.422833e-54
MsG0380015877.01	MsG0780040679.01	0.862617	4.464072e-64	1.315411e-60
MsG0380015877.01	MsG0780040704.01	0.823347	1.454549e-53	1.249111e-50
MsG0380015877.01	MsG0780041485.01	0.880175	6.814112e-70	3.800992e-66
MsG0380015877.01	MsG0780041753.01	0.808637	2.808991e-50	1.614370e-47
MsG0380015877.01	MsG0780041777.01	0.889049	3.428168e-73	2.722429e-69
MsG0380015877.01	MsG0880042965.01	0.814018	1.907554e-51	1.265482e-48
MsG0380015877.01	MsG0880042967.01	0.813274	2.781069e-51	1.808024e-48
MsG0380015877.01	MsG0880045106.01	0.808397	3.159966e-50	1.804908e-47
MsG0380015877.01	MsG0880045314.01	0.804347	2.257041e-49	1.161010e-46
MsG0380015877.01	MsG0880045372.01	0.801632	8.218017e-49	3.943741e-46
MsG0380015877.01	MsG0880045590.01	0.834686	2.596883e-56	3.104319e-53
MsG0380015877.01	MsG0880045639.01	0.806162	9.405501e-50	5.069382e-47
MsG0380015877.01	MsG0880045782.01	0.806655	7.400974e-50	4.039178e-47
MsG0380015877.01	MsG0880045827.01	0.807006	6.238787e-50	3.436120e-47
MsG0380015877.01	MsG0880045888.01	0.810037	1.406605e-50	8.390542e-48
MsG0380015877.01	MsG0880046020.01	0.807593	4.687018e-50	2.620938e-47
MsG0380015877.01	MsG0880046023.01	0.814455	1.527099e-51	1.025157e-48
MsG0380015877.01	MsG0880046440.01	0.827398	1.598995e-54	1.542081e-51
MsG0380015877.01	MsG0880046586.01	0.801922	7.162190e-49	3.462561e-46
MsG0380015877.01	MsG0880046642.01	0.827540	1.478878e-54	1.431883e-51
MsG0380015877.01	MsG0880046731.01	0.801266	9.762488e-49	4.642085e-46
MsG0380015877.01	MsG0880047337.01	0.802277	6.056360e-49	2.954568e-46
MsG0280006510.01	MsG0380015877.01	0.803973	2.699448e-49	1.375352e-46
MsG0280006586.01	MsG0380015877.01	0.825601	4.288615e-54	3.927316e-51
MsG0280006781.01	MsG0380015877.01	0.804410	2.189201e-49	1.127922e-46
MsG0280007481.01	MsG0380015877.01	0.884360	2.049092e-71	1.347797e-67
MsG0280007529.01	MsG0380015877.01	0.802821	4.677678e-49	2.313937e-46
MsG0280007826.01	MsG0380015877.01	0.803533	3.330985e-49	1.678076e-46
MsG0280008073.01	MsG0380015877.01	0.895331	1.060408e-75	1.095435e-71
MsG0280008220.01	MsG0380015877.01	0.825011	5.915592e-54	5.327219e-51
MsG0280008270.01	MsG0380015877.01	0.812453	4.208124e-51	2.676848e-48
MsG0280008295.01	MsG0380015877.01	0.821123	4.772048e-53	3.848636e-50
MsG0280008384.01	MsG0380015877.01	0.801713	7.906284e-49	3.802034e-46
MsG0280008615.01	MsG0380015877.01	0.801306	9.583435e-49	4.561501e-46
MsG0280009024.01	MsG0380015877.01	0.820382	7.063741e-53	5.580908e-50
MsG0280010170.01	MsG0380015877.01	0.825527	4.464899e-54	4.079907e-51
MsG0280010459.01	MsG0380015877.01	0.805358	1.387266e-49	7.324438e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015877.01.T01	MTR_3g079340	92.308	65	5	0	1	65	413	477	3.71e-37	130
MsG0380015877.01.T01	MTR_3g078633	57.971	69	28	1	1	69	345	412	2.46e-18	77.8
MsG0380015877.01.T01	MTR_3g078633	57.971	69	28	1	1	69	417	484	2.49e-18	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015877.01.T01	AT3G48090	50.847	59	28	1	5	63	393	450	4.12e-14	65.9
MsG0380015877.01.T01	AT3G48090	50.847	59	28	1	5	63	285	342	4.41e-14	65.9
MsG0380015877.01.T01	AT3G48080	42.424	66	37	1	4	69	397	461	9.09e-13	62.0

Find 15 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACTTGTGCTTGCAGGAGTA+TGG	0.270068	3:-76200152	MsG0380015877.01.T01:CDS
CGTCCCAAGCATGAGGGATT+TGG	0.356445	3:-76200274	None:intergenic
ACAAGTCTCTTCACGTTGCT+TGG	0.364881	3:+76200168	None:intergenic
ACGTTGCTTGGGAAATCCTT+TGG	0.372544	3:+76200180	None:intergenic
GTGAAGAGACTTGTGCTTGC+AGG	0.386249	3:-76200159	MsG0380015877.01.T01:CDS
CAAGTCTCTTCACGTTGCTT+GGG	0.408050	3:+76200169	None:intergenic
TGATGCCTTCAAGGTGCAAA+AGG	0.480497	3:-76200205	MsG0380015877.01.T01:CDS
TGTGAGATAAACAATGGAAA+AGG	0.505004	3:-76200234	MsG0380015877.01.T01:CDS
AGGTTATTATGATGCCTTCA+AGG	0.524131	3:-76200214	MsG0380015877.01.T01:CDS
CTAGAGGCTCAACAAGTTGA+CGG	0.528764	3:+76200044	None:intergenic
AACTGCCAGATGAGTTTGAA+GGG	0.559752	3:-76200098	MsG0380015877.01.T01:CDS
ACTTGTGCTTGCAGGAGTAT+GGG	0.592966	3:-76200151	MsG0380015877.01.T01:CDS
CAAGGTGCAAAAGGATCCAA+AGG	0.597796	3:-76200196	MsG0380015877.01.T01:CDS
GAACTGCCAGATGAGTTTGA+AGG	0.604489	3:-76200099	MsG0380015877.01.T01:CDS
TCAACATGTGAGATAAACAA+TGG	0.616431	3:-76200240	MsG0380015877.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TCAACATGTGAGATAAACAA+TGG	-	Chr3:76200068-76200087	MsG0380015877.01.T01:CDS	30.0%
	TGTGAGATAAACAATGGAAA+AGG	-	Chr3:76200074-76200093	MsG0380015877.01.T01:CDS	30.0%
!!	GAAAAAGAATGGATTGAACT+CGG	-	Chr3:76200232-76200251	MsG0380015877.01.T01:CDS	30.0%
	AGGTTATTATGATGCCTTCA+AGG	-	Chr3:76200094-76200113	MsG0380015877.01.T01:CDS	35.0%
	GAGTTTGAAGGGAAAAAGAA+TGG	-	Chr3:76200221-76200240	MsG0380015877.01.T01:CDS	35.0%
!	TTTTTCCCTTCAAACTCATC+TGG	+	Chr3:76200218-76200237	None:intergenic	35.0%
	AACTGCCAGATGAGTTTGAA+GGG	-	Chr3:76200210-76200229	MsG0380015877.01.T01:CDS	40.0%
	ACAAGTCTCTTCACGTTGCT+TGG	+	Chr3:76200143-76200162	None:intergenic	45.0%
	ACGTTGCTTGGGAAATCCTT+TGG	+	Chr3:76200131-76200150	None:intergenic	45.0%
	CAAGGTGCAAAAGGATCCAA+AGG	-	Chr3:76200112-76200131	MsG0380015877.01.T01:CDS	45.0%
	CAAGTCTCTTCACGTTGCTT+GGG	+	Chr3:76200142-76200161	None:intergenic	45.0%
	GAACTGCCAGATGAGTTTGA+AGG	-	Chr3:76200209-76200228	MsG0380015877.01.T01:CDS	45.0%
	TGATGCCTTCAAGGTGCAAA+AGG	-	Chr3:76200103-76200122	MsG0380015877.01.T01:CDS	45.0%
!	ACTTGTGCTTGCAGGAGTAT+GGG	-	Chr3:76200157-76200176	MsG0380015877.01.T01:CDS	45.0%
!	CTAGAGGCTCAACAAGTTGA+CGG	+	Chr3:76200267-76200286	None:intergenic	45.0%
!	TGGATCCTTTTGCACCTTGA+AGG	+	Chr3:76200111-76200130	None:intergenic	45.0%
	GACTTGTGCTTGCAGGAGTA+TGG	-	Chr3:76200156-76200175	MsG0380015877.01.T01:CDS	50.0%
	GTGAAGAGACTTGTGCTTGC+AGG	-	Chr3:76200149-76200168	MsG0380015877.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	76200044	76200286	76200044	ID=MsG0380015877.01;Name=MsG0380015877.01
Chr3	mRNA	76200044	76200286	76200044	ID=MsG0380015877.01.T01;Parent=MsG0380015877.01;Name=MsG0380015877.01.T01;_AED=0.49;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|80
Chr3	exon	76200044	76200286	76200044	ID=MsG0380015877.01.T01:exon:1168;Parent=MsG0380015877.01.T01
Chr3	CDS	76200044	76200286	76200044	ID=MsG0380015877.01.T01:cds;Parent=MsG0380015877.01.T01

Gene Sequence	>MsG0380015877.01.T01 ATGAGGGATTTGGAAGAGTACAAATCAACATGTGAGATAAACAATGGAAAAGGTTATTATGATGCCTTCAAGGTGCAAAAGGATCCAAAGGATTTCCCAAGCAACGTGAAGAGACTTGTGCTTGCAGGAGTATGGGATGAGATCATTGAAATGCTGAGAGAGTATGAACTGCCAGATGAGTTTGAAGGGAAAAAGAATGGATTGAACTCGGAACGAGATTCCGTCAACTTGTTGAGCCTCTAG
Protein sequence	>MsG0380015877.01.T01 MRDLEEYKSTCEINNGKGYYDAFKVQKDPKDFPSNVKRLVLAGVWDEIIEMLREYELPDEFEGKKNGLNSERDSVNLLSL*