Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034123.01.T01 | VVW90989.1 | 92.157 | 102 | 8 | 0 | 10 | 111 | 98 | 199 | 1.77E-57 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034123.01.T01 | A4QJI4 | 93.137 | 102 | 7 | 0 | 10 | 111 | 296 | 397 | 1.60E-57 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034123.01.T01 | A0A5K1HNR2 | 92.157 | 102 | 8 | 0 | 10 | 111 | 98 | 199 | 8.46e-58 | 187 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024908.01 | MsG0680034123.01 | 0.863791 | 1.934818e-64 | 5.939254e-61 |
| MsG0380016059.01 | MsG0680034123.01 | 0.839209 | 1.817184e-57 | 2.491905e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034123.01.T01 | MTR_0002s0550 | 91.176 | 102 | 9 | 0 | 10 | 111 | 296 | 397 | 1.98e-56 | 182 |
| MsG0680034123.01.T01 | MTR_2g008350 | 84.158 | 101 | 16 | 0 | 11 | 111 | 78 | 178 | 4.21e-53 | 168 |
| MsG0680034123.01.T01 | MTR_0212s0040 | 73.529 | 102 | 27 | 0 | 10 | 111 | 306 | 407 | 3.80e-43 | 150 |
| MsG0680034123.01.T01 | MTR_3g108400 | 80.952 | 63 | 12 | 0 | 10 | 72 | 29 | 91 | 4.65e-31 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034123.01.T01 | ATCG00120 | 92.157 | 102 | 8 | 0 | 10 | 111 | 296 | 397 | 5.35e-58 | 186 |
| MsG0680034123.01.T01 | ATMG01190 | 72.549 | 102 | 28 | 0 | 10 | 111 | 306 | 407 | 5.18e-43 | 147 |
| MsG0680034123.01.T01 | AT2G07698 | 72.549 | 102 | 28 | 0 | 10 | 111 | 576 | 677 | 6.41e-42 | 146 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGAGTATTTCTCGTCTTT+TGG | 0.183483 | 6:-81123974 | MsG0680034123.01.T01:CDS |
| GGTAGCTGGTAAATTAAAAT+TGG | 0.278592 | 6:-81123716 | MsG0680034123.01.T01:CDS |
| TTTACCAGCTACCTGTTTCA+TGG | 0.322039 | 6:+81123726 | None:intergenic |
| AAGACGAGAAATACTCTTGT+TGG | 0.362910 | 6:+81123978 | None:intergenic |
| CCTGATTGGGTCTCAACTAT+TGG | 0.368964 | 6:+81123898 | None:intergenic |
| GAGAAATACTCTTGTTGGTT+TGG | 0.376780 | 6:+81123983 | None:intergenic |
| TGGTAAATTAAAATTGGAAT+TGG | 0.382867 | 6:-81123710 | MsG0680034123.01.T01:CDS |
| CCAATAGTTGAGACCCAATC+AGG | 0.395916 | 6:-81123898 | MsG0680034123.01.T01:CDS |
| TCAAATTAAAGCCATGAAAC+AGG | 0.408465 | 6:-81123737 | MsG0680034123.01.T01:CDS |
| AAGCTGAAACATCTCCTGAT+TGG | 0.409535 | 6:+81123884 | None:intergenic |
| AATACTCTTGTTGGTTTGGT+AGG | 0.428583 | 6:+81123987 | None:intergenic |
| AAATATTTGGCCATCTGTAA+TGG | 0.434228 | 6:+81123840 | None:intergenic |
| GTAATGGAAATTACATTAGT+AGG | 0.444650 | 6:+81123856 | None:intergenic |
| AGCTGAAACATCTCCTGATT+GGG | 0.452206 | 6:+81123885 | None:intergenic |
| TTTGCCGATCTATTCAATGC+TGG | 0.492613 | 6:-81123814 | MsG0680034123.01.T01:CDS |
| TTGAGCCGTCGATCGAACTC+TGG | 0.500203 | 6:+81123756 | None:intergenic |
| GAAATACCCACATTAATAGC+TGG | 0.538501 | 6:+81123784 | None:intergenic |
| CGTCGATCGAACTCTGGAAA+CGG | 0.554205 | 6:+81123762 | None:intergenic |
| AATGTAATTTCCATTACAGA+TGG | 0.557336 | 6:-81123850 | MsG0680034123.01.T01:CDS |
| AAAGCCATGAAACAGGTAGC+TGG | 0.558354 | 6:-81123730 | MsG0680034123.01.T01:CDS |
| CGTTTCCAGAGTTCGATCGA+CGG | 0.561362 | 6:-81123761 | MsG0680034123.01.T01:CDS |
| ATAAATTCTCAGTTAAGTGA+AGG | 0.595218 | 6:-81123937 | MsG0680034123.01.T01:CDS |
| ATCAGACCAGCTATTAATGT+GGG | 0.604091 | 6:-81123790 | MsG0680034123.01.T01:CDS |
| GATTCCAGCATTGAATAGAT+CGG | 0.616835 | 6:+81123810 | None:intergenic |
| AATCAGACCAGCTATTAATG+TGG | 0.625394 | 6:-81123791 | MsG0680034123.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGGTAAATTAAAATTGGAAT+TGG | - | Chr6:81123959-81123978 | MsG0680034123.01.T01:CDS | 20.0% |
| !!! | TAACTGAGAATTTATTTTAC+CGG | + | Chr6:81123727-81123746 | None:intergenic | 20.0% |
| ! | AATGTAATTTCCATTACAGA+TGG | - | Chr6:81123819-81123838 | MsG0680034123.01.T01:CDS | 25.0% |
| ! | ATAAATTCTCAGTTAAGTGA+AGG | - | Chr6:81123732-81123751 | MsG0680034123.01.T01:CDS | 25.0% |
| ! | GATCGGCAAATAAAAATATT+TGG | + | Chr6:81123845-81123864 | None:intergenic | 25.0% |
| !! | GTAATGGAAATTACATTAGT+AGG | + | Chr6:81123816-81123835 | None:intergenic | 25.0% |
| AAATATTTGGCCATCTGTAA+TGG | + | Chr6:81123832-81123851 | None:intergenic | 30.0% | |
| GGTAGCTGGTAAATTAAAAT+TGG | - | Chr6:81123953-81123972 | MsG0680034123.01.T01:CDS | 30.0% | |
| TCAAATTAAAGCCATGAAAC+AGG | - | Chr6:81123932-81123951 | MsG0680034123.01.T01:CDS | 30.0% | |
| AAGACGAGAAATACTCTTGT+TGG | + | Chr6:81123694-81123713 | None:intergenic | 35.0% | |
| AATCAGACCAGCTATTAATG+TGG | - | Chr6:81123878-81123897 | MsG0680034123.01.T01:CDS | 35.0% | |
| ATCAGACCAGCTATTAATGT+GGG | - | Chr6:81123879-81123898 | MsG0680034123.01.T01:CDS | 35.0% | |
| GAAATACCCACATTAATAGC+TGG | + | Chr6:81123888-81123907 | None:intergenic | 35.0% | |
| ! | GAGAAATACTCTTGTTGGTT+TGG | + | Chr6:81123689-81123708 | None:intergenic | 35.0% |
| ! | GATTCCAGCATTGAATAGAT+CGG | + | Chr6:81123862-81123881 | None:intergenic | 35.0% |
| !! | AATACTCTTGTTGGTTTGGT+AGG | + | Chr6:81123685-81123704 | None:intergenic | 35.0% |
| !!! | CAAGAGTATTTCTCGTCTTT+TGG | - | Chr6:81123695-81123714 | MsG0680034123.01.T01:CDS | 35.0% |
| AAGCTGAAACATCTCCTGAT+TGG | + | Chr6:81123788-81123807 | None:intergenic | 40.0% | |
| AGCTGAAACATCTCCTGATT+GGG | + | Chr6:81123787-81123806 | None:intergenic | 40.0% | |
| TTTACCAGCTACCTGTTTCA+TGG | + | Chr6:81123946-81123965 | None:intergenic | 40.0% | |
| TTTGCCGATCTATTCAATGC+TGG | - | Chr6:81123855-81123874 | MsG0680034123.01.T01:CDS | 40.0% | |
| AAAGCCATGAAACAGGTAGC+TGG | - | Chr6:81123939-81123958 | MsG0680034123.01.T01:CDS | 45.0% | |
| CCAATAGTTGAGACCCAATC+AGG | - | Chr6:81123771-81123790 | MsG0680034123.01.T01:CDS | 45.0% | |
| CCTGATTGGGTCTCAACTAT+TGG | + | Chr6:81123774-81123793 | None:intergenic | 45.0% | |
| !! | TCTCGTCTTTTGGAAAGAGC+CGG | - | Chr6:81123705-81123724 | MsG0680034123.01.T01:CDS | 45.0% |
| CGTTTCCAGAGTTCGATCGA+CGG | - | Chr6:81123908-81123927 | MsG0680034123.01.T01:CDS | 50.0% | |
| ! | CGTCGATCGAACTCTGGAAA+CGG | + | Chr6:81123910-81123929 | None:intergenic | 50.0% |
| TTGAGCCGTCGATCGAACTC+TGG | + | Chr6:81123916-81123935 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 81123678 | 81124013 | 81123678 | ID=MsG0680034123.01;Name=MsG0680034123.01 |
| Chr6 | mRNA | 81123678 | 81124013 | 81123678 | ID=MsG0680034123.01.T01;Parent=MsG0680034123.01;Name=MsG0680034123.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|111 |
| Chr6 | exon | 81123678 | 81124013 | 81123678 | ID=MsG0680034123.01.T01:exon:11842;Parent=MsG0680034123.01.T01 |
| Chr6 | CDS | 81123678 | 81124013 | 81123678 | ID=MsG0680034123.01.T01:cds;Parent=MsG0680034123.01.T01 |
| Gene Sequence |
| Protein sequence |
