AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034421.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034421.01.T01 MTR_6g088270 85.405 185 27 0 114 298 844 1028 3.03e-100 317
MsG0680034421.01.T01 MTR_6g088270 50.820 305 106 2 38 298 483 787 3.25e-86 279
MsG0680034421.01.T01 MTR_6g087300 66.120 183 33 1 114 296 204 357 4.01e-67 214
MsG0680034421.01.T01 MTR_6g087300 50.000 78 39 0 161 238 50 127 2.39e-18 84.7
MsG0680034421.01.T01 MTR_2g039780 68.493 146 31 2 154 298 60 191 1.51e-58 187
MsG0680034421.01.T01 MTR_6g084360 66.667 147 20 1 152 298 974 1091 1.68e-49 176
MsG0680034421.01.T01 MTR_6g088240 56.805 169 66 4 137 298 1 169 4.22e-49 162
MsG0680034421.01.T01 MTR_0428s0020 54.545 187 28 4 114 299 38 168 1.03e-47 159
MsG0680034421.01.T01 MTR_2g039790 73.874 111 16 1 114 224 15 112 3.27e-46 153
MsG0680034421.01.T01 MTR_6g488290 53.297 182 28 4 114 294 38 163 3.41e-44 149
MsG0680034421.01.T01 MTR_6g027070 51.333 150 54 2 120 269 13 143 1.98e-39 135
MsG0680034421.01.T01 MTR_6g088250 76.136 88 21 0 114 201 1255 1342 1.35e-34 133
MsG0680034421.01.T01 MTR_6g003970 45.794 107 55 2 113 218 20 124 1.89e-17 82.0
MsG0680034421.01.T01 MTR_6g066260 44.330 97 50 2 148 242 3 97 2.05e-17 76.6
MsG0680034421.01.T01 MTR_6g088250 55.385 65 21 1 55 111 1121 1185 2.13e-13 71.2
MsG0680034421.01.T01 MTR_6g081090 56.923 65 24 1 114 178 5 65 2.73e-13 65.9
MsG0680034421.01.T01 MTR_6g087190 74.359 39 10 0 168 206 15 53 9.49e-13 62.8
MsG0680034421.01.T01 MTR_6g087200 42.683 82 37 1 180 261 1033 1104 3.69e-12 67.4
MsG0680034421.01.T01 MTR_0428s0040 82.353 34 6 0 66 99 84 117 4.86e-11 61.2
MsG0680034421.01.T01 MTR_6g087320 60.377 53 21 0 47 99 1373 1425 6.04e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034421.01.T01 AT4G29060 73.626 182 48 0 117 298 754 935 1.06e-83 271
MsG0680034421.01.T01 AT4G29060 67.598 179 58 0 120 298 519 697 1.29e-75 249
MsG0680034421.01.T01 AT4G29060 68.156 179 51 1 120 298 519 691 3.18e-76 246
MsG0680034421.01.T01 AT4G11120 44.538 119 57 4 93 207 46 159 7.70e-17 80.5

Find 77 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGAAATTTGTATAATTT+TGG 0.154806 6:+87405061 MsG0680034421.01.T01:three_prime_UTR
CTTTCAATCTAATTATCTAT+TGG 0.232376 6:+87404998 MsG0680034421.01.T01:three_prime_UTR
TATAGAGCAGCCTTTCATTA+AGG 0.293654 6:+87404877 MsG0680034421.01.T01:CDS
TCCTTCAGCTGCTAGTCTTC+CGG 0.310289 6:-87404561 None:intergenic
TAGTAACCATGGTGGAATTC+TGG 0.332567 6:-87405148 None:intergenic
TTAATTGAAAACAAAAGGTT+TGG 0.345084 6:+87405295 MsG0680034421.01.T01:three_prime_UTR
GGAAAATCTTTGTTGGAGAT+TGG 0.350910 6:+87401968 MsG0680034421.01.T01:CDS
AGAAACCGGTGCAGGGATAA+TGG 0.359757 6:+87404445 MsG0680034421.01.T01:CDS
GACCAAGGAAGTGTTATAAA+TGG 0.367748 6:+87402109 MsG0680034421.01.T01:CDS
CTAGCAGCTGAAGGAAGAAT+TGG 0.394602 6:+87404569 MsG0680034421.01.T01:CDS
ATCCATTAATTGAAAACAAA+AGG 0.396239 6:+87405290 MsG0680034421.01.T01:three_prime_UTR
GCAGATTCAAATTGCACAAA+TGG 0.403499 6:-87404945 None:intergenic
TATAATGGGAAAATCTTTGT+TGG 0.419087 6:+87401961 None:intergenic
CATGGTTACTACACAGAAAA+CGG 0.435582 6:+87405160 MsG0680034421.01.T01:three_prime_UTR
CACCATTTATAACACTTCCT+TGG 0.437574 6:-87402111 None:intergenic
GGGAGAATCTCAAAGAGGTT+AGG 0.459157 6:+87404842 MsG0680034421.01.T01:CDS
AGCATCGGTGTTCTAATTGA+AGG 0.459553 6:+87404611 MsG0680034421.01.T01:CDS
TAAACTTTCAGAAGCCGCTT+CGG 0.465962 6:-87402149 None:intergenic
CCTGAGGGAAGCTTGAATAA+AGG 0.470228 6:+87402652 MsG0680034421.01.T01:CDS
GATTGACAACACTGATTGTC+AGG 0.470813 6:-87402706 None:intergenic
CAGGCAAGACGGATTTGAAA+AGG 0.478246 6:-87402687 None:intergenic
GAGATTGATGCTAGTTTGAC+TGG 0.484458 6:+87402610 MsG0680034421.01.T01:CDS
TTATCCGCTGATAAGAAATC+CGG 0.489725 6:+87404542 MsG0680034421.01.T01:CDS
GAAATCAACGGTAATGGTCT+CGG 0.490019 6:+87404397 MsG0680034421.01.T01:CDS
GCATCAATCTCATTGTTCTC+AGG 0.492520 6:-87402598 None:intergenic
GCAAGTGTTTGCTTGTCCAC+AGG 0.492659 6:+87404700 MsG0680034421.01.T01:CDS
GATTTCAACAATATGAGAAC+AGG 0.495196 6:+87405124 MsG0680034421.01.T01:three_prime_UTR
CCTTTATTCAAGCTTCCCTC+AGG 0.497257 6:-87402652 None:intergenic
CTGCGAGAAGAAACCGGTGC+AGG 0.501344 6:+87404437 MsG0680034421.01.T01:CDS
GGTAATGGTCTCGGCCTCAT+TGG 0.509725 6:+87404406 MsG0680034421.01.T01:CDS
ATGGTTACTACACAGAAAAC+GGG 0.510099 6:+87405161 MsG0680034421.01.T01:three_prime_UTR
ACGGTAAAAGCTGTGACATA+TGG 0.511431 6:+87405241 MsG0680034421.01.T01:three_prime_UTR
CGGCAGAAGTGAGAAATTCA+AGG 0.519937 6:+87404655 MsG0680034421.01.T01:CDS
AAGCAACTGCGAGAAGAAAC+CGG 0.521340 6:+87404431 MsG0680034421.01.T01:CDS
CTCACTTCTGCCGACAAAGT+CGG 0.524777 6:-87404645 None:intergenic
TTGCAGGAAATCAACGGTAA+TGG 0.535839 6:+87404391 MsG0680034421.01.T01:intron
ATCATCCGCAACAGTTGTTG+AGG 0.540849 6:-87402080 None:intergenic
AGTAACCTTGTGTTCGCTCA+AGG 0.546576 6:+87405193 MsG0680034421.01.T01:three_prime_UTR
ATAACAGAGAAAATTGTTGA+GGG 0.548214 6:+87404821 MsG0680034421.01.T01:CDS
GAAGGAAGGAGCTAGTTCAT+TGG 0.552591 6:+87402051 MsG0680034421.01.T01:CDS
AACTGTGAAACCGACTTTGT+CGG 0.552624 6:+87404635 MsG0680034421.01.T01:CDS
TGTTCTCGGGTTTCAAAGCA+AGG 0.552660 6:-87404799 None:intergenic
GAAAGCTCTTTCTGAAACAG+AGG 0.553530 6:+87404475 MsG0680034421.01.T01:CDS
AGCGACGTTGATTACGAAGA+AGG 0.555840 6:+87402033 MsG0680034421.01.T01:CDS
CGAGATTTGCGTATCACAAG+AGG 0.557273 6:+87402007 MsG0680034421.01.T01:CDS
TCTTGAAACCATTGTGACGA+AGG 0.558013 6:+87404748 MsG0680034421.01.T01:CDS
TTTCAAGTAGATGACGATGT+TGG 0.558946 6:+87402568 MsG0680034421.01.T01:intron
TACAATCACGATTCAAGCAT+CGG 0.562819 6:+87404596 MsG0680034421.01.T01:CDS
CATTTCCTTGAGCGAACACA+AGG 0.570437 6:-87405198 None:intergenic
CGGTTCCTCAACAACTGTTG+CGG 0.573354 6:+87402075 MsG0680034421.01.T01:CDS
TATAAATGGTGCCACTGAGA+AGG 0.575300 6:+87402123 MsG0680034421.01.T01:CDS
CATAACAGAGAAAATTGTTG+AGG 0.576710 6:+87404820 MsG0680034421.01.T01:CDS
ACAGTCCATTATCCCTGCAC+CGG 0.579376 6:-87404450 None:intergenic
CAGTGTTACTGTGAAATACT+TGG 0.584020 6:+87404904 MsG0680034421.01.T01:CDS
CGCTTCGGTTTCCTTCTCAG+TGG 0.591068 6:-87402134 None:intergenic
AACAGAGGGTGATCTTGTAA+AGG 0.592332 6:+87404490 MsG0680034421.01.T01:CDS
ACACTGTCATCCTTAATGAA+AGG 0.601630 6:-87404887 None:intergenic
AAAGCTCTTTCTGAAACAGA+GGG 0.603411 6:+87404476 MsG0680034421.01.T01:CDS
GCATCGGTGTTCTAATTGAA+GGG 0.603411 6:+87404612 MsG0680034421.01.T01:CDS
AAGTGATGATTTGTCTCCTG+AGG 0.605174 6:+87402636 MsG0680034421.01.T01:CDS
TCCGGAAGACTAGCAGCTGA+AGG 0.605549 6:+87404560 MsG0680034421.01.T01:CDS
TGCGAGAAGAAACCGGTGCA+GGG 0.608538 6:+87404438 MsG0680034421.01.T01:CDS
TCGCAGTTGCTTAACCAATG+AGG 0.608617 6:-87404420 None:intergenic
ACAGATACAAACTGAACCTG+TGG 0.609443 6:-87404716 None:intergenic
GAAGGAGCTAGTTCATTGGT+CGG 0.610923 6:+87402055 MsG0680034421.01.T01:CDS
ACACTGATTGTCAGGCAAGA+CGG 0.614543 6:-87402698 None:intergenic
ACGTTGATTACGAAGAAGGA+AGG 0.614789 6:+87402037 MsG0680034421.01.T01:CDS
AGTGATGATTTGTCTCCTGA+GGG 0.629295 6:+87402637 MsG0680034421.01.T01:CDS
GCGGATGATGAAAGCGACCA+AGG 0.634703 6:+87402094 MsG0680034421.01.T01:CDS
TCTTCCGGATTTCTTATCAG+CGG 0.637237 6:-87404546 None:intergenic
TTGAGGGGAGAATCTCAAAG+AGG 0.648053 6:+87404837 MsG0680034421.01.T01:CDS
CACTGAGAAGGAAACCGAAG+CGG 0.663126 6:+87402135 MsG0680034421.01.T01:CDS
TAACAGAGAAAATTGTTGAG+GGG 0.674904 6:+87404822 MsG0680034421.01.T01:CDS
GTTCTGTTGCAGGAAATCAA+CGG 0.691087 6:+87404385 MsG0680034421.01.T01:intron
TTTCTGTGTAGTAACCATGG+TGG 0.698240 6:-87405156 None:intergenic
TTACTGTGAAATACTTGGTG+AGG 0.700350 6:+87404909 MsG0680034421.01.T01:CDS
ACAGGTCCAGAATTCCACCA+TGG 0.745439 6:+87405142 MsG0680034421.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGAAATTTGTATAATTT+TGG + Chr6:87405061-87405080 MsG0680034421.01.T01:three_prime_UTR 10.0%
!! AACATATATATAAGTATAGA+GGG - Chr6:87404101-87404120 None:intergenic 15.0%
!! AAGAAAAATTGAGAAAAAAA+TGG + Chr6:87404330-87404349 MsG0680034421.01.T01:intron 15.0%
!! AATTATGGAAATGTTATAAT+GGG - Chr6:87404024-87404043 None:intergenic 15.0%
!! TAACATATATATAAGTATAG+AGG - Chr6:87404102-87404121 None:intergenic 15.0%
!! TAATTATGGAAATGTTATAA+TGG - Chr6:87404025-87404044 None:intergenic 15.0%
!! TATATTATCTGAATTATTCT+AGG + Chr6:87404122-87404141 MsG0680034421.01.T01:intron 15.0%
!! AATGGAAAGGAAATATTATT+TGG - Chr6:87402550-87402569 None:intergenic 20.0%
!! ATACATATATTCATACTGTT+TGG + Chr6:87403313-87403332 MsG0680034421.01.T01:intron 20.0%
!! ATCCATTAATTGAAAACAAA+AGG + Chr6:87405290-87405309 MsG0680034421.01.T01:three_prime_UTR 20.0%
!! CTTTCAATCTAATTATCTAT+TGG + Chr6:87404998-87405017 MsG0680034421.01.T01:three_prime_UTR 20.0%
!! TTAATTGAAAACAAAAGGTT+TGG + Chr6:87405295-87405314 MsG0680034421.01.T01:three_prime_UTR 20.0%
!!! AACATACATTTTCATGTATA+AGG - Chr6:87405269-87405288 None:intergenic 20.0%
!!! AACCTTTTGTTTTCAATTAA+TGG - Chr6:87405295-87405314 None:intergenic 20.0%
!!! ACATACATTTTCATGTATAA+GGG - Chr6:87405268-87405287 None:intergenic 20.0%
!!! ATGTAATGTACCTTTTAAAA+TGG - Chr6:87403727-87403746 None:intergenic 20.0%
!!! CTGATATTTTTTGTATACTT+TGG - Chr6:87405105-87405124 None:intergenic 20.0%
!!! GTAATATTCTTTTTTTGTCA+CGG + Chr6:87405222-87405241 MsG0680034421.01.T01:three_prime_UTR 20.0%
! ATAACAGAGAAAATTGTTGA+GGG + Chr6:87404821-87404840 MsG0680034421.01.T01:CDS 25.0%
! CAACAATCATAAACTGATAA+TGG + Chr6:87402322-87402341 MsG0680034421.01.T01:intron 25.0%
! CCATAATAAAACACAGTTAA+AGG + Chr6:87403138-87403157 MsG0680034421.01.T01:intron 25.0%
! TACACAATCAATTAATGATC+AGG - Chr6:87403861-87403880 None:intergenic 25.0%
! TACATAAAGATCAAATAGAC+AGG - Chr6:87402924-87402943 None:intergenic 25.0%
! TATACATACACACTTATATG+AGG + Chr6:87402187-87402206 MsG0680034421.01.T01:intron 25.0%
! TATATATAAGTATAGAGGGA+AGG - Chr6:87404097-87404116 None:intergenic 25.0%
! TCAAATAAGTCAATCCAAAA+GGG - Chr6:87403340-87403359 None:intergenic 25.0%
! TTATGGAAATAAGCTGAAAA+CGG - Chr6:87403440-87403459 None:intergenic 25.0%
! TTCAAATAAGTCAATCCAAA+AGG - Chr6:87403341-87403360 None:intergenic 25.0%
! TTGTTCATCAGAATTAGTAA+AGG - Chr6:87403170-87403189 None:intergenic 25.0%
!! AACAACACTAGATTGAATTT+TGG + Chr6:87402861-87402880 MsG0680034421.01.T01:intron 25.0%
!! AACTATCGTGTTAATTTTGA+CGG - Chr6:87403808-87403827 None:intergenic 25.0%
!! AATTTTCTCTGTTATGTTCT+CGG - Chr6:87404816-87404835 None:intergenic 25.0%
!! TTGCATAAAGTTGATTGATT+GGG - Chr6:87402995-87403014 None:intergenic 25.0%
!! TTTGCATAAAGTTGATTGAT+TGG - Chr6:87402996-87403015 None:intergenic 25.0%
!!! ATCATAAACTGATAATGGAA+AGG + Chr6:87402327-87402346 MsG0680034421.01.T01:intron 25.0%
!!! CCTTTAACTGTGTTTTATTA+TGG - Chr6:87403141-87403160 None:intergenic 25.0%
!!! GAAACTTTGAATTAAGTTGA+AGG - Chr6:87403584-87403603 None:intergenic 25.0%
!!! GGTTGTTTTGTTTAAAATGT+AGG + Chr6:87403658-87403677 MsG0680034421.01.T01:intron 25.0%
AATTATGCCATGCTTTATCA+GGG + Chr6:87404040-87404059 MsG0680034421.01.T01:intron 30.0%
AGACTGTTTGGAAGAATTTA+TGG + Chr6:87403387-87403406 MsG0680034421.01.T01:intron 30.0%
ATCAATTAATGATCAGGACA+AGG - Chr6:87403855-87403874 None:intergenic 30.0%
ATTACATCTTCAGTTCATCA+AGG + Chr6:87403740-87403759 MsG0680034421.01.T01:intron 30.0%
ATTTGACACAAACAAGATCA+AGG - Chr6:87403615-87403634 None:intergenic 30.0%
CAAATAAGTCAATCCAAAAG+GGG - Chr6:87403339-87403358 None:intergenic 30.0%
CATAACAGAGAAAATTGTTG+AGG + Chr6:87404820-87404839 MsG0680034421.01.T01:CDS 30.0%
CCATATGTAAAATACGAATG+CGG - Chr6:87402459-87402478 None:intergenic 30.0%
CTAAAAACTGTAACAGAAGT+CGG + Chr6:87403881-87403900 MsG0680034421.01.T01:intron 30.0%
CTTAATTCAAAGTTTCAAGC+AGG + Chr6:87403588-87403607 MsG0680034421.01.T01:intron 30.0%
GATAAAGCATGGCATAATTA+TGG - Chr6:87404039-87404058 None:intergenic 30.0%
GATTTCAACAATATGAGAAC+AGG + Chr6:87405124-87405143 MsG0680034421.01.T01:three_prime_UTR 30.0%
GTACAATATCATGCATTATG+TGG - Chr6:87402951-87402970 None:intergenic 30.0%
GTCATTTCTTCATGTCAATT+TGG + Chr6:87403637-87403656 MsG0680034421.01.T01:intron 30.0%
TAAAACACAGTTAAAGGTGA+AGG + Chr6:87403144-87403163 MsG0680034421.01.T01:intron 30.0%
TAACAGAGAAAATTGTTGAG+GGG + Chr6:87404822-87404841 MsG0680034421.01.T01:CDS 30.0%
TAATTATGCCATGCTTTATC+AGG + Chr6:87404039-87404058 MsG0680034421.01.T01:intron 30.0%
TACACATACACACTTATATG+AGG + Chr6:87403545-87403564 MsG0680034421.01.T01:intron 30.0%
! AATCTAGTGTTGTTACATAG+TGG - Chr6:87402855-87402874 None:intergenic 30.0%
! ATTTTCTCTGTTATGTTCTC+GGG - Chr6:87404815-87404834 None:intergenic 30.0%
!!! AAGCTTTTTTAGACAGCTTA+TGG - Chr6:87403457-87403476 None:intergenic 30.0%
AAGTCGGCTAAGAATAAAGT+TGG + Chr6:87403897-87403916 MsG0680034421.01.T01:intron 35.0%
ACACTGTCATCCTTAATGAA+AGG - Chr6:87404890-87404909 None:intergenic 35.0%
ACATCGTCATCTACTTGAAA+TGG - Chr6:87402568-87402587 None:intergenic 35.0%
ATAAGTATAGAGGGAAGGTT+CGG - Chr6:87404092-87404111 None:intergenic 35.0%
ATGATGTCATTCCATTGCTT+TGG - Chr6:87403019-87403038 None:intergenic 35.0%
ATGGTTACTACACAGAAAAC+GGG + Chr6:87405161-87405180 MsG0680034421.01.T01:three_prime_UTR 35.0%
ATTGTTCAATGTTCTGTTGC+AGG + Chr6:87404375-87404394 MsG0680034421.01.T01:intron 35.0%
CAAGCATACCTCAGAAAAAA+GGG + Chr6:87404515-87404534 MsG0680034421.01.T01:CDS 35.0%
CACCATTTATAACACTTCCT+TGG - Chr6:87402114-87402133 None:intergenic 35.0%
CATGGTTACTACACAGAAAA+CGG + Chr6:87405160-87405179 MsG0680034421.01.T01:three_prime_UTR 35.0%
CTGAAAGTTTAGCTGTTGAA+GGG + Chr6:87402161-87402180 MsG0680034421.01.T01:intron 35.0%
CTTTATGCAAACCAAAGCAA+TGG + Chr6:87403005-87403024 MsG0680034421.01.T01:intron 35.0%
GCAGATTCAAATTGCACAAA+TGG - Chr6:87404948-87404967 None:intergenic 35.0%
GGAAAATCTTTGTTGGAGAT+TGG + Chr6:87401968-87401987 MsG0680034421.01.T01:CDS 35.0%
GGGATCATATATGCAATGAA+AGG - Chr6:87402975-87402994 None:intergenic 35.0%
GTCATCTACTTGAAATGGAA+AGG - Chr6:87402563-87402582 None:intergenic 35.0%
GTCTGCTATCACATTTACTT+CGG - Chr6:87403285-87403304 None:intergenic 35.0%
GTTCATATAGCAGCTATAGT+GGG - Chr6:87402788-87402807 None:intergenic 35.0%
GTTGCATCTAAGTTACATCA+TGG + Chr6:87403762-87403781 MsG0680034421.01.T01:intron 35.0%
TAAGAACTTGTGAGACTGTT+TGG + Chr6:87403375-87403394 MsG0680034421.01.T01:intron 35.0%
TACAATCACGATTCAAGCAT+CGG + Chr6:87404596-87404615 MsG0680034421.01.T01:CDS 35.0%
TATAGAGCAGCCTTTCATTA+AGG + Chr6:87404877-87404896 MsG0680034421.01.T01:CDS 35.0%
TCTGAAAGTTTAGCTGTTGA+AGG + Chr6:87402160-87402179 MsG0680034421.01.T01:intron 35.0%
TGAAAGTTTAGCTGTTGAAG+GGG + Chr6:87402162-87402181 MsG0680034421.01.T01:intron 35.0%
TGTTCATATAGCAGCTATAG+TGG - Chr6:87402789-87402808 None:intergenic 35.0%
TTATCACAGAATCCTCAAGA+AGG + Chr6:87402392-87402411 MsG0680034421.01.T01:intron 35.0%
TTATCCGCTGATAAGAAATC+CGG + Chr6:87404542-87404561 MsG0680034421.01.T01:CDS 35.0%
TTCATATAGCAGCTATAGTG+GGG - Chr6:87402787-87402806 None:intergenic 35.0%
TTGAGGATTCTGTGATAACA+GGG - Chr6:87402390-87402409 None:intergenic 35.0%
! AAAATGGTCAGCAAGACTTT+TGG - Chr6:87403711-87403730 None:intergenic 35.0%
! CAGTGTTACTGTGAAATACT+TGG + Chr6:87404904-87404923 MsG0680034421.01.T01:CDS 35.0%
! CCGCATTCGTATTTTACATA+TGG + Chr6:87402456-87402475 MsG0680034421.01.T01:intron 35.0%
! GATGAAAGTGTAACAGAAAG+TGG + Chr6:87402271-87402290 MsG0680034421.01.T01:intron 35.0%
! GTCTTGCTGACCATTTTAAA+AGG + Chr6:87403714-87403733 MsG0680034421.01.T01:intron 35.0%
! TAAGCTGAAAACGGCTTTTA+TGG - Chr6:87403431-87403450 None:intergenic 35.0%
! TTACTGTGAAATACTTGGTG+AGG + Chr6:87404909-87404928 MsG0680034421.01.T01:CDS 35.0%
! TTTCAAGTAGATGACGATGT+TGG + Chr6:87402568-87402587 MsG0680034421.01.T01:intron 35.0%
! TTTTCTTTCCTTCGTCACAA+TGG - Chr6:87404759-87404778 None:intergenic 35.0%
!! AAAGCTCTTTCTGAAACAGA+GGG + Chr6:87404476-87404495 MsG0680034421.01.T01:CDS 35.0%
!! GACCAAGGAAGTGTTATAAA+TGG + Chr6:87402109-87402128 MsG0680034421.01.T01:CDS 35.0%
!! TCAAATGTCCGTTTTTGTGT+TGG - Chr6:87404212-87404231 None:intergenic 35.0%
!! TGAAAGCACCAACACAAAAA+CGG + Chr6:87404201-87404220 MsG0680034421.01.T01:intron 35.0%
!!! AACTGCTATTTTCTGCGATA+TGG - Chr6:87402732-87402751 None:intergenic 35.0%
!!! ACTGCTATTTTCTGCGATAT+GGG - Chr6:87402731-87402750 None:intergenic 35.0%
!!! ATAAGAGACCCTTTTTTCTG+AGG - Chr6:87404526-87404545 None:intergenic 35.0%
AACAGAGGGTGATCTTGTAA+AGG + Chr6:87404490-87404509 MsG0680034421.01.T01:CDS 40.0%
ACAACTAACACCTACACATG+TGG - Chr6:87404300-87404319 None:intergenic 40.0%
ACAGATACAAACTGAACCTG+TGG - Chr6:87404719-87404738 None:intergenic 40.0%
ACGGTAAAAGCTGTGACATA+TGG + Chr6:87405241-87405260 MsG0680034421.01.T01:three_prime_UTR 40.0%
ACTTATATGAGGAGACTGCA+AGG + Chr6:87403556-87403575 MsG0680034421.01.T01:intron 40.0%
AGCATTACTGCTTCAGATGA+AGG + Chr6:87403193-87403212 MsG0680034421.01.T01:intron 40.0%
CTGAACATAACCACATGTGT+AGG + Chr6:87404287-87404306 MsG0680034421.01.T01:intron 40.0%
CTTATATGAGGAGACTGCAA+GGG + Chr6:87403557-87403576 MsG0680034421.01.T01:intron 40.0%
CTTGAGGATTCTGTGATAAC+AGG - Chr6:87402391-87402410 None:intergenic 40.0%
GCAAGCATACCTCAGAAAAA+AGG + Chr6:87404514-87404533 MsG0680034421.01.T01:CDS 40.0%
GCATCAATCTCATTGTTCTC+AGG - Chr6:87402601-87402620 None:intergenic 40.0%
GCTATAGTGCTATAGCATAG+TGG - Chr6:87402831-87402850 None:intergenic 40.0%
GCTGTAATGCTATTGCATAG+CGG - Chr6:87402765-87402784 None:intergenic 40.0%
GTCATACACATTCACAGCTT+AGG - Chr6:87404164-87404183 None:intergenic 40.0%
GTTCTGTTGCAGGAAATCAA+CGG + Chr6:87404385-87404404 MsG0680034421.01.T01:intron 40.0%
TAAACTTTCAGAAGCCGCTT+CGG - Chr6:87402152-87402171 None:intergenic 40.0%
TAAGTATAGAGGGAAGGTTC+GGG - Chr6:87404091-87404110 None:intergenic 40.0%
TCTTCAGTTGTAGGACTATC+AGG - Chr6:87403114-87403133 None:intergenic 40.0%
TCTTCCGGATTTCTTATCAG+CGG - Chr6:87404549-87404568 None:intergenic 40.0%
TCTTGAAACCATTGTGACGA+AGG + Chr6:87404748-87404767 MsG0680034421.01.T01:CDS 40.0%
TTGCAGGAAATCAACGGTAA+TGG + Chr6:87404391-87404410 MsG0680034421.01.T01:intron 40.0%
TTTCTGTGTAGTAACCATGG+TGG - Chr6:87405159-87405178 None:intergenic 40.0%
! AACTGTGAAACCGACTTTGT+CGG + Chr6:87404635-87404654 MsG0680034421.01.T01:CDS 40.0%
! AAGTGATGATTTGTCTCCTG+AGG + Chr6:87402636-87402655 MsG0680034421.01.T01:CDS 40.0%
! ACGTTGATTACGAAGAAGGA+AGG + Chr6:87402037-87402056 MsG0680034421.01.T01:CDS 40.0%
! AGTGATGATTTGTCTCCTGA+GGG + Chr6:87402637-87402656 MsG0680034421.01.T01:CDS 40.0%
! CGTTTTCTGTGTAGTAACCA+TGG - Chr6:87405162-87405181 None:intergenic 40.0%
! GAAATCAACGGTAATGGTCT+CGG + Chr6:87404397-87404416 MsG0680034421.01.T01:CDS 40.0%
! GAGATTGATGCTAGTTTGAC+TGG + Chr6:87402610-87402629 MsG0680034421.01.T01:CDS 40.0%
! GATTGACAACACTGATTGTC+AGG - Chr6:87402709-87402728 None:intergenic 40.0%
! GCATCGGTGTTCTAATTGAA+GGG + Chr6:87404612-87404631 MsG0680034421.01.T01:CDS 40.0%
! TAATGGGCAAAACTACAACG+AGG - Chr6:87404008-87404027 None:intergenic 40.0%
! TAGTAACCATGGTGGAATTC+TGG - Chr6:87405151-87405170 None:intergenic 40.0%
! TGAAATCGCAACCTTTTCTC+AGG - Chr6:87403219-87403238 None:intergenic 40.0%
!! AGCATCGGTGTTCTAATTGA+AGG + Chr6:87404611-87404630 MsG0680034421.01.T01:CDS 40.0%
!! GAAAGCTCTTTCTGAAACAG+AGG + Chr6:87404475-87404494 MsG0680034421.01.T01:CDS 40.0%
!! TATAAATGGTGCCACTGAGA+AGG + Chr6:87402123-87402142 MsG0680034421.01.T01:CDS 40.0%
AAGCAACTGCGAGAAGAAAC+CGG + Chr6:87404431-87404450 MsG0680034421.01.T01:CDS 45.0%
AGTAACCTTGTGTTCGCTCA+AGG + Chr6:87405193-87405212 MsG0680034421.01.T01:three_prime_UTR 45.0%
AGTTGTAGGACTATCAGGAG+AGG - Chr6:87403109-87403128 None:intergenic 45.0%
ATCATCCGCAACAGTTGTTG+AGG - Chr6:87402083-87402102 None:intergenic 45.0%
CAAGAACCTTCTCCTTCTTG+AGG - Chr6:87402407-87402426 None:intergenic 45.0%
CAGAATCCTCAAGAAGGAGA+AGG + Chr6:87402398-87402417 MsG0680034421.01.T01:intron 45.0%
CATTTCCTTGAGCGAACACA+AGG - Chr6:87405201-87405220 None:intergenic 45.0%
CCTGAGGGAAGCTTGAATAA+AGG + Chr6:87402652-87402671 MsG0680034421.01.T01:CDS 45.0%
CCTTTATTCAAGCTTCCCTC+AGG - Chr6:87402655-87402674 None:intergenic 45.0%
CGAGATTTGCGTATCACAAG+AGG + Chr6:87402007-87402026 MsG0680034421.01.T01:CDS 45.0%
CGGCAGAAGTGAGAAATTCA+AGG + Chr6:87404655-87404674 MsG0680034421.01.T01:CDS 45.0%
CTAGCAGCTGAAGGAAGAAT+TGG + Chr6:87404569-87404588 MsG0680034421.01.T01:CDS 45.0%
CTTAGCACCCTGATAAAGCA+TGG - Chr6:87404050-87404069 None:intergenic 45.0%
GAAGGAAGGAGCTAGTTCAT+TGG + Chr6:87402051-87402070 MsG0680034421.01.T01:CDS 45.0%
GAAGGAGCTAGTTCATTGGT+CGG + Chr6:87402055-87402074 MsG0680034421.01.T01:CDS 45.0%
GGGAGAATCTCAAAGAGGTT+AGG + Chr6:87404842-87404861 MsG0680034421.01.T01:CDS 45.0%
GGTTCAACTGTCTCTGTTAC+AGG - Chr6:87402364-87402383 None:intergenic 45.0%
GTAACAGAGACAGTTGAACC+TGG + Chr6:87402364-87402383 MsG0680034421.01.T01:intron 45.0%
GTTGTAGGACTATCAGGAGA+GGG - Chr6:87403108-87403127 None:intergenic 45.0%
TAACGCTGACATGTCGACAT+TGG + Chr6:87404240-87404259 MsG0680034421.01.T01:intron 45.0%
TATGGCGCTTCTTCAGTTGT+AGG - Chr6:87403123-87403142 None:intergenic 45.0%
TCAGATGAAGGCCTGAGAAA+AGG + Chr6:87403205-87403224 MsG0680034421.01.T01:intron 45.0%
TCGCAGTTGCTTAACCAATG+AGG - Chr6:87404423-87404442 None:intergenic 45.0%
TGTTCTCGGGTTTCAAAGCA+AGG - Chr6:87404802-87404821 None:intergenic 45.0%
TTGAGGGGAGAATCTCAAAG+AGG + Chr6:87404837-87404856 MsG0680034421.01.T01:CDS 45.0%
! AACTGACTCACTGGTTGAAG+TGG + Chr6:87402246-87402265 MsG0680034421.01.T01:intron 45.0%
! ACACTGATTGTCAGGCAAGA+CGG - Chr6:87402701-87402720 None:intergenic 45.0%
! AGCGACGTTGATTACGAAGA+AGG + Chr6:87402033-87402052 MsG0680034421.01.T01:CDS 45.0%
! CAATGTCGAAACTGACTCAC+TGG + Chr6:87402237-87402256 MsG0680034421.01.T01:intron 45.0%
! CAGGCAAGACGGATTTGAAA+AGG - Chr6:87402690-87402709 None:intergenic 45.0%
! TCATACTGTTTGGCCCCTTT+TGG + Chr6:87403323-87403342 MsG0680034421.01.T01:intron 45.0%
ACAGGTCCAGAATTCCACCA+TGG + Chr6:87405142-87405161 MsG0680034421.01.T01:three_prime_UTR 50.0%
ACAGTCCATTATCCCTGCAC+CGG - Chr6:87404453-87404472 None:intergenic 50.0%
AGAAACCGGTGCAGGGATAA+TGG + Chr6:87404445-87404464 MsG0680034421.01.T01:CDS 50.0%
CACTGAGAAGGAAACCGAAG+CGG + Chr6:87402135-87402154 MsG0680034421.01.T01:CDS 50.0%
CGGTTCCTCAACAACTGTTG+CGG + Chr6:87402075-87402094 MsG0680034421.01.T01:CDS 50.0%
CTCACTTCTGCCGACAAAGT+CGG - Chr6:87404648-87404667 None:intergenic 50.0%
GATTCTGTGATAACAGGGCC+AGG - Chr6:87402385-87402404 None:intergenic 50.0%
GCAAGTGTTTGCTTGTCCAC+AGG + Chr6:87404700-87404719 MsG0680034421.01.T01:CDS 50.0%
!! TCCTTCAGCTGCTAGTCTTC+CGG - Chr6:87404564-87404583 None:intergenic 50.0%
CGCTTCGGTTTCCTTCTCAG+TGG - Chr6:87402137-87402156 None:intergenic 55.0%
GCGGATGATGAAAGCGACCA+AGG + Chr6:87402094-87402113 MsG0680034421.01.T01:CDS 55.0%
TCCGGAAGACTAGCAGCTGA+AGG + Chr6:87404560-87404579 MsG0680034421.01.T01:CDS 55.0%
TGCGAGAAGAAACCGGTGCA+GGG + Chr6:87404438-87404457 MsG0680034421.01.T01:CDS 55.0%
! GGTAATGGTCTCGGCCTCAT+TGG + Chr6:87404406-87404425 MsG0680034421.01.T01:CDS 55.0%
CTGCGAGAAGAAACCGGTGC+AGG + Chr6:87404437-87404456 MsG0680034421.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 87401965 87405322 87401965 ID=MsG0680034421.01;Name=MsG0680034421.01
Chr6 mRNA 87401965 87405322 87401965 ID=MsG0680034421.01.T01;Parent=MsG0680034421.01;Name=MsG0680034421.01.T01;_AED=0.44;_eAED=0.46;_QI=0|0|0.33|1|1|1|3|361|301
Chr6 exon 87401965 87402166 87401965 ID=MsG0680034421.01.T01:exon:18293;Parent=MsG0680034421.01.T01
Chr6 exon 87402578 87402716 87402578 ID=MsG0680034421.01.T01:exon:18294;Parent=MsG0680034421.01.T01
Chr6 exon 87404397 87405322 87404397 ID=MsG0680034421.01.T01:exon:18295;Parent=MsG0680034421.01.T01
Chr6 CDS 87401965 87402166 87401965 ID=MsG0680034421.01.T01:cds;Parent=MsG0680034421.01.T01
Chr6 CDS 87402578 87402716 87402578 ID=MsG0680034421.01.T01:cds;Parent=MsG0680034421.01.T01
Chr6 CDS 87404397 87404961 87404397 ID=MsG0680034421.01.T01:cds;Parent=MsG0680034421.01.T01
Chr6 three_prime_UTR 87404962 87405322 87404962 ID=MsG0680034421.01.T01:three_prime_utr;Parent=MsG0680034421.01.T01
Gene Sequence

>MsG0680034421.01.T01

ATGGGAAAATCTTTGTTGGAGATTGGTCAAGAAGTTAGCGTACGAGATTTGCGTATCACAAGAGGACAAGCGACGTTGATTACGAAGAAGGAAGGAGCTAGTTCATTGGTCGGTTCCTCAACAACTGTTGCGGATGATGAAAGCGACCAAGGAAGTGTTATAAATGGTGCCACTGAGAAGGAAACCGAAGCGGCTTCTGAAAATGACGATGTTGGAGCTGTTCCTGAGAACAATGAGATTGATGCTAGTTTGACTGGACAAAGTGATGATTTGTCTCCTGAGGGAAGCTTGAATAAAGGTTATGCTAGTTTCCTTTTCAAATCCGTCTTGCCTGACAATCAGAAATCAACGGTAATGGTCTCGGCCTCATTGGTTAAGCAACTGCGAGAAGAAACCGGTGCAGGGATAATGGACTGTAAGAAAGCTCTTTCTGAAACAGAGGGTGATCTTGTAAAGGTGCAAGCATACCTCAGAAAAAAGGGTCTCTTATCCGCTGATAAGAAATCCGGAAGACTAGCAGCTGAAGGAAGAATTGGTACATACAATCACGATTCAAGCATCGGTGTTCTAATTGAAGGGAACTGTGAAACCGACTTTGTCGGCAGAAGTGAGAAATTCAAGGAGTGTGTTGATGATCTTGCAATGCAAGTGTTTGCTTGTCCACAGGTTCAGTTTGTATCTGTTGAAGTTATTCTTGAAACCATTGTGACGAAGGAAAGAAAACTTGCGTTGCAGAGAGAAGACCTTGCTTTGAAACCCGAGAACATAACAGAGAAAATTGTTGAGGGGAGAATCTCAAAGAGGTTAGGAGATCTTGCTCTTATAGAGCAGCCTTTCATTAAGGATGACAGTGTTACTGTGAAATACTTGGTGAGGCAAAGTATAGCTGCCATTTGTGCAATTTGA
Protein sequence

>MsG0680034421.01.T01

MGKSLLEIGQEVSVRDLRITRGQATLITKKEGASSLVGSSTTVADDESDQGSVINGATEKETEAASENDDVGAVPENNEIDASLTGQSDDLSPEGSLNKGYASFLFKSVLPDNQKSTVMVSASLVKQLREETGAGIMDCKKALSETEGDLVKVQAYLRKKGLLSADKKSGRLAAEGRIGTYNHDSSIGVLIEGNCETDFVGRSEKFKECVDDLAMQVFACPQVQFVSVEVILETIVTKERKLALQREDLALKPENITEKIVEGRISKRLGDLALIEQPFIKDDSVTVKYLVRQSIAAICAI*