Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034997.01.T01 | XP_024641447.2 | 60.227 | 176 | 56 | 2 | 1 | 169 | 936 | 1104 | 1.69E-56 | 202 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034997.01.T01 | G7KL58 | 60.227 | 176 | 56 | 2 | 1 | 169 | 979 | 1147 | 9.10e-57 | 202 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680032801.01 | MsG0680034997.01 | 0.806126 | 9.571988e-50 | 5.154298e-47 |
| MsG0680034997.01 | MsG0680035005.01 | 0.803333 | 3.665708e-49 | 1.837085e-46 |
| MsG0680034997.01 | MsG0780036128.01 | 0.825417 | 4.740588e-54 | 4.318306e-51 |
| MsG0680034997.01 | MsG0880042161.01 | 0.806143 | 9.490884e-50 | 5.112829e-47 |
| MsG0680034997.01 | MsG0880046771.01 | 0.802884 | 4.539987e-49 | 2.249280e-46 |
| MsG0480021587.01 | MsG0680034997.01 | 0.800438 | 1.440939e-48 | 6.709570e-46 |
| MsG0380017220.01 | MsG0680034997.01 | 0.808250 | 3.397258e-50 | 1.932980e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034997.01.T01 | MTR_6g078890 | 60.227 | 176 | 56 | 2 | 1 | 169 | 979 | 1147 | 2.31e-60 | 202 |
| MsG0680034997.01.T01 | MTR_6g079090 | 58.011 | 181 | 58 | 4 | 1 | 174 | 1113 | 1282 | 1.55e-56 | 191 |
| MsG0680034997.01.T01 | MTR_3g006280 | 55.978 | 184 | 65 | 4 | 2 | 178 | 1360 | 1534 | 1.96e-55 | 188 |
| MsG0680034997.01.T01 | MTR_6g079120 | 58.960 | 173 | 67 | 3 | 2 | 173 | 967 | 1136 | 1.28e-54 | 186 |
| MsG0680034997.01.T01 | MTR_6g079140 | 56.886 | 167 | 68 | 3 | 1 | 166 | 1115 | 1278 | 1.50e-54 | 186 |
| MsG0680034997.01.T01 | MTR_0012s0290 | 55.556 | 171 | 72 | 3 | 1 | 170 | 853 | 1020 | 2.68e-51 | 176 |
| MsG0680034997.01.T01 | MTR_0012s0290 | 55.556 | 171 | 72 | 3 | 1 | 170 | 971 | 1138 | 4.37e-51 | 176 |
| MsG0680034997.01.T01 | MTR_6g078930 | 55.000 | 180 | 68 | 4 | 2 | 176 | 1286 | 1457 | 1.37e-48 | 169 |
| MsG0680034997.01.T01 | MTR_6g077640 | 56.129 | 155 | 64 | 3 | 1 | 154 | 1095 | 1246 | 1.61e-48 | 168 |
| MsG0680034997.01.T01 | MTR_6g078480 | 53.333 | 180 | 66 | 4 | 2 | 174 | 855 | 1023 | 6.02e-48 | 167 |
| MsG0680034997.01.T01 | MTR_6g078480 | 53.333 | 180 | 66 | 4 | 2 | 174 | 1033 | 1201 | 7.18e-48 | 166 |
| MsG0680034997.01.T01 | MTR_6g079000 | 50.267 | 187 | 75 | 5 | 1 | 178 | 1535 | 1712 | 7.53e-48 | 166 |
| MsG0680034997.01.T01 | MTR_1g019550 | 45.251 | 179 | 76 | 5 | 3 | 172 | 1411 | 1576 | 4.11e-38 | 139 |
| MsG0680034997.01.T01 | MTR_1g019550 | 45.251 | 179 | 76 | 5 | 3 | 172 | 1444 | 1609 | 4.12e-38 | 139 |
| MsG0680034997.01.T01 | MTR_1g019550 | 45.251 | 179 | 76 | 5 | 3 | 172 | 1267 | 1432 | 4.54e-38 | 138 |
| MsG0680034997.01.T01 | MTR_8g032830 | 53.103 | 145 | 54 | 2 | 1 | 138 | 890 | 1027 | 5.30e-36 | 132 |
| MsG0680034997.01.T01 | MTR_6g078980 | 45.342 | 161 | 68 | 3 | 4 | 164 | 145 | 285 | 2.53e-32 | 117 |
| MsG0680034997.01.T01 | MTR_0007s0150 | 42.857 | 119 | 52 | 4 | 2 | 114 | 401 | 509 | 2.35e-16 | 76.3 |
| MsG0680034997.01.T01 | MTR_6g008140 | 33.516 | 182 | 99 | 6 | 2 | 166 | 1027 | 1203 | 2.24e-15 | 73.6 |
| MsG0680034997.01.T01 | MTR_6g008140 | 33.516 | 182 | 99 | 6 | 2 | 166 | 851 | 1027 | 2.33e-15 | 73.6 |
| MsG0680034997.01.T01 | MTR_6g008140 | 33.516 | 182 | 99 | 6 | 2 | 166 | 697 | 873 | 2.54e-15 | 73.2 |
| MsG0680034997.01.T01 | MTR_6g008140 | 33.516 | 182 | 99 | 6 | 2 | 166 | 712 | 888 | 2.69e-15 | 73.2 |
| MsG0680034997.01.T01 | MTR_6g075970 | 33.702 | 181 | 100 | 7 | 2 | 169 | 878 | 1051 | 5.36e-15 | 72.4 |
| MsG0680034997.01.T01 | MTR_0038s0080 | 33.702 | 181 | 100 | 7 | 2 | 169 | 878 | 1051 | 5.36e-15 | 72.4 |
| MsG0680034997.01.T01 | MTR_8g075440 | 32.558 | 172 | 103 | 6 | 1 | 166 | 875 | 1039 | 1.31e-14 | 71.2 |
| MsG0680034997.01.T01 | MTR_6g477750 | 34.426 | 183 | 91 | 9 | 2 | 169 | 46 | 214 | 7.58e-13 | 65.1 |
| MsG0680034997.01.T01 | MTR_8g042470 | 30.055 | 183 | 103 | 5 | 2 | 167 | 916 | 1090 | 1.31e-12 | 65.5 |
| MsG0680034997.01.T01 | MTR_6g076090 | 31.818 | 176 | 101 | 9 | 2 | 165 | 871 | 1039 | 1.36e-12 | 65.5 |
| MsG0680034997.01.T01 | MTR_8g042470 | 30.220 | 182 | 102 | 5 | 3 | 167 | 917 | 1090 | 1.74e-12 | 65.1 |
| MsG0680034997.01.T01 | MTR_8g042470 | 30.220 | 182 | 102 | 5 | 3 | 167 | 917 | 1090 | 1.89e-12 | 65.1 |
| MsG0680034997.01.T01 | MTR_5g038180 | 33.146 | 178 | 104 | 7 | 2 | 166 | 881 | 1056 | 2.39e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGATTGTCGATTTCTTTC+TGG | 0.095201 | 6:-97482883 | MsG0680034997.01.T01:CDS |
| AACCATGCAGAGGTTGATTT+TGG | 0.193099 | 6:-97482631 | MsG0680034997.01.T01:CDS |
| CGAGAACCATACCATGTTAT+TGG | 0.235343 | 6:+97482820 | None:intergenic |
| CAAAATGAAATTACTAAATC+AGG | 0.375434 | 6:-97483259 | MsG0680034997.01.T01:intron |
| CGCATTGGTGGTTAAACCTC+TGG | 0.386918 | 6:+97482909 | None:intergenic |
| AAGCACTTTCAGAAAATTTG+TGG | 0.386995 | 6:-97482654 | MsG0680034997.01.T01:CDS |
| ACAATGTTAGCCTCATACTC+TGG | 0.407689 | 6:+97482535 | None:intergenic |
| TGTCCGTGGAAAACGGAAAA+CGG | 0.442015 | 6:+97482938 | None:intergenic |
| CTATACAAATAGTTTGAAGA+TGG | 0.447930 | 6:-97482755 | MsG0680034997.01.T01:CDS |
| GAATATTTGTCCGTGGAAAA+CGG | 0.463181 | 6:+97482931 | None:intergenic |
| CTTCACCAACCCAGAGTATG+AGG | 0.477099 | 6:-97482545 | MsG0680034997.01.T01:CDS |
| TTTAACCACCAATGCGAGGC+AGG | 0.481023 | 6:-97482902 | MsG0680034997.01.T01:CDS |
| ATTTCTCCAATAACATGGTA+TGG | 0.490542 | 6:-97482826 | MsG0680034997.01.T01:CDS |
| ACAATCCTGCCTCGCATTGG+TGG | 0.502010 | 6:+97482897 | None:intergenic |
| AAATGACACAGAGTAATTGC+AGG | 0.503564 | 6:+97482845 | None:intergenic |
| GGATTACAATGCCAGAAAAG+TGG | 0.531833 | 6:-97482610 | MsG0680034997.01.T01:CDS |
| TAACAGAAATTACATGAGGA+TGG | 0.532932 | 6:-97482973 | MsG0680034997.01.T01:intron |
| TCGACAATCCTGCCTCGCAT+TGG | 0.548425 | 6:+97482894 | None:intergenic |
| GAAAGATAAAAGCAGCATGA+AGG | 0.553921 | 6:-97482575 | MsG0680034997.01.T01:CDS |
| ACCTCTGGAATATTTGTCCG+TGG | 0.575648 | 6:+97482924 | None:intergenic |
| CAATGTTAGCCTCATACTCT+GGG | 0.584632 | 6:+97482536 | None:intergenic |
| GTTAGCCTCATACTCTGGGT+TGG | 0.597416 | 6:+97482540 | None:intergenic |
| ATCCAAAATCAACCTCTGCA+TGG | 0.604451 | 6:+97482629 | None:intergenic |
| GGAAAACTTCAATGATAACA+TGG | 0.610559 | 6:-97482680 | MsG0680034997.01.T01:CDS |
| TCCACGGACAAATATTCCAG+AGG | 0.615666 | 6:-97482925 | MsG0680034997.01.T01:CDS |
| TGAATAACAGAAATTACATG+AGG | 0.629193 | 6:-97482977 | MsG0680034997.01.T01:intron |
| GTGTCATTTCTCCAATAACA+TGG | 0.634416 | 6:-97482831 | MsG0680034997.01.T01:CDS |
| AAATTTGTGGAACCATGCAG+AGG | 0.658869 | 6:-97482641 | MsG0680034997.01.T01:CDS |
| GAGGTTTAACCACCAATGCG+AGG | 0.664022 | 6:-97482906 | MsG0680034997.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATACATAACAAATAA+GGG | + | Chr6:97482781-97482800 | None:intergenic | 10.0% |
| !! | TATAAAATACATAACAAATA+AGG | + | Chr6:97482782-97482801 | None:intergenic | 10.0% |
| !!! | TTATTTTGTATTTATTTGTT+GGG | - | Chr6:97482587-97482606 | MsG0680034997.01.T01:CDS | 10.0% |
| !!! | TTTATTTTGTATTTATTTGT+TGG | - | Chr6:97482586-97482605 | MsG0680034997.01.T01:CDS | 10.0% |
| !! | CAAAATGAAATTACTAAATC+AGG | - | Chr6:97482557-97482576 | MsG0680034997.01.T01:CDS | 20.0% |
| ! | AATACATGTAATCAATGCAT+TGG | - | Chr6:97482671-97482690 | MsG0680034997.01.T01:CDS | 25.0% |
| ! | CTATACAAATAGTTTGAAGA+TGG | - | Chr6:97483061-97483080 | MsG0680034997.01.T01:intron | 25.0% |
| ! | TGAATAACAGAAATTACATG+AGG | - | Chr6:97482839-97482858 | MsG0680034997.01.T01:CDS | 25.0% |
| !! | TGCTTTTATCTTTCAATACA+TGG | + | Chr6:97483235-97483254 | None:intergenic | 25.0% |
| !!! | GCATCTTTTTCATTTGAAAA+TGG | - | Chr6:97483115-97483134 | MsG0680034997.01.T01:intron | 25.0% |
| AAGCACTTTCAGAAAATTTG+TGG | - | Chr6:97483162-97483181 | MsG0680034997.01.T01:intron | 30.0% | |
| ATTTCTCCAATAACATGGTA+TGG | - | Chr6:97482990-97483009 | MsG0680034997.01.T01:intron | 30.0% | |
| GGAAAACTTCAATGATAACA+TGG | - | Chr6:97483136-97483155 | MsG0680034997.01.T01:intron | 30.0% | |
| TAACAGAAATTACATGAGGA+TGG | - | Chr6:97482843-97482862 | MsG0680034997.01.T01:CDS | 30.0% | |
| AAATGACACAGAGTAATTGC+AGG | + | Chr6:97482974-97482993 | None:intergenic | 35.0% | |
| GAAAGATAAAAGCAGCATGA+AGG | - | Chr6:97483241-97483260 | MsG0680034997.01.T01:intron | 35.0% | |
| GAATATTTGTCCGTGGAAAA+CGG | + | Chr6:97482888-97482907 | None:intergenic | 35.0% | |
| !! | ATACATGGATTCCACTTTTC+TGG | + | Chr6:97483220-97483239 | None:intergenic | 35.0% |
| !! | GTGTCATTTCTCCAATAACA+TGG | - | Chr6:97482985-97483004 | MsG0680034997.01.T01:intron | 35.0% |
| AAATTTGTGGAACCATGCAG+AGG | - | Chr6:97483175-97483194 | MsG0680034997.01.T01:intron | 40.0% | |
| ATCCAAAATCAACCTCTGCA+TGG | + | Chr6:97483190-97483209 | None:intergenic | 40.0% | |
| CGAGAACCATACCATGTTAT+TGG | + | Chr6:97482999-97483018 | None:intergenic | 40.0% | |
| GGATTACAATGCCAGAAAAG+TGG | - | Chr6:97483206-97483225 | MsG0680034997.01.T01:intron | 40.0% | |
| ! | ACAATGTTAGCCTCATACTC+TGG | + | Chr6:97483284-97483303 | None:intergenic | 40.0% |
| ! | CAATGTTAGCCTCATACTCT+GGG | + | Chr6:97483283-97483302 | None:intergenic | 40.0% |
| !! | AACCATGCAGAGGTTGATTT+TGG | - | Chr6:97483185-97483204 | MsG0680034997.01.T01:intron | 40.0% |
| !! | CAGGATTGTCGATTTCTTTC+TGG | - | Chr6:97482933-97482952 | MsG0680034997.01.T01:CDS | 40.0% |
| TCCACGGACAAATATTCCAG+AGG | - | Chr6:97482891-97482910 | MsG0680034997.01.T01:CDS | 45.0% | |
| TGTCCGTGGAAAACGGAAAA+CGG | + | Chr6:97482881-97482900 | None:intergenic | 45.0% | |
| ! | GTTCCGTTTTCCGTTTTCCA+CGG | - | Chr6:97482875-97482894 | MsG0680034997.01.T01:CDS | 45.0% |
| !! | ACCTCTGGAATATTTGTCCG+TGG | + | Chr6:97482895-97482914 | None:intergenic | 45.0% |
| !! | AGTATGCTTAAAGAGTGCCC+CGG | - | Chr6:97482737-97482756 | MsG0680034997.01.T01:CDS | 45.0% |
| !! | ATAGTCCTTAAAGAGTGCCC+CGG | + | Chr6:97482631-97482650 | None:intergenic | 45.0% |
| AAATGTTAACGAGTGCCCCC+GGG | - | Chr6:97482610-97482629 | MsG0680034997.01.T01:CDS | 50.0% | |
| CTTCACCAACCCAGAGTATG+AGG | - | Chr6:97483271-97483290 | MsG0680034997.01.T01:CDS | 50.0% | |
| GAAATGTTAACGAGTGCCCC+CGG | - | Chr6:97482609-97482628 | MsG0680034997.01.T01:CDS | 50.0% | |
| GAGGTTTAACCACCAATGCG+AGG | - | Chr6:97482910-97482929 | MsG0680034997.01.T01:CDS | 50.0% | |
| TTTAACCACCAATGCGAGGC+AGG | - | Chr6:97482914-97482933 | MsG0680034997.01.T01:CDS | 50.0% | |
| ! | CGCATTGGTGGTTAAACCTC+TGG | + | Chr6:97482910-97482929 | None:intergenic | 50.0% |
| ! | GTTAGCCTCATACTCTGGGT+TGG | + | Chr6:97483279-97483298 | None:intergenic | 50.0% |
| !! | GTATGCTTAAAGAGTGCCCC+GGG | - | Chr6:97482738-97482757 | MsG0680034997.01.T01:CDS | 50.0% |
| !! | TAGTCCTTAAAGAGTGCCCC+GGG | + | Chr6:97482630-97482649 | None:intergenic | 50.0% |
| !! | TATGCTTAAAGAGTGCCCCG+GGG | - | Chr6:97482739-97482758 | MsG0680034997.01.T01:CDS | 50.0% |
| AATGTTAACGAGTGCCCCCG+GGG | - | Chr6:97482611-97482630 | MsG0680034997.01.T01:CDS | 55.0% | |
| ACAATCCTGCCTCGCATTGG+TGG | + | Chr6:97482922-97482941 | None:intergenic | 55.0% | |
| TCGACAATCCTGCCTCGCAT+TGG | + | Chr6:97482925-97482944 | None:intergenic | 55.0% | |
| TCTTGTTAACGAGTGCCCCC+GGG | + | Chr6:97482758-97482777 | None:intergenic | 55.0% | |
| ! | GTCTTGTTAACGAGTGCCCC+CGG | + | Chr6:97482759-97482778 | None:intergenic | 55.0% |
| !! | AGTCCTTAAAGAGTGCCCCG+GGG | + | Chr6:97482629-97482648 | None:intergenic | 55.0% |
| !! | ATGCTTAAAGAGTGCCCCGG+GGG | - | Chr6:97482740-97482759 | MsG0680034997.01.T01:CDS | 55.0% |
| CTTGTTAACGAGTGCCCCCG+GGG | + | Chr6:97482757-97482776 | None:intergenic | 60.0% | |
| !! | GTCCTTAAAGAGTGCCCCGG+GGG | + | Chr6:97482628-97482647 | None:intergenic | 60.0% |
| ! | TGCCCCCGGGGCACTCTTTA+AGG | - | Chr6:97482623-97482642 | MsG0680034997.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 97482516 | 97483322 | 97482516 | ID=MsG0680034997.01;Name=MsG0680034997.01 |
| Chr6 | mRNA | 97482516 | 97483322 | 97482516 | ID=MsG0680034997.01.T01;Parent=MsG0680034997.01;Name=MsG0680034997.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|0|0|2|0|178 |
| Chr6 | exon | 97482516 | 97482989 | 97482516 | ID=MsG0680034997.01.T01:exon:13899;Parent=MsG0680034997.01.T01 |
| Chr6 | exon | 97483260 | 97483322 | 97483260 | ID=MsG0680034997.01.T01:exon:13898;Parent=MsG0680034997.01.T01 |
| Chr6 | CDS | 97483260 | 97483322 | 97483260 | ID=MsG0680034997.01.T01:cds;Parent=MsG0680034997.01.T01 |
| Chr6 | CDS | 97482516 | 97482989 | 97482516 | ID=MsG0680034997.01.T01:cds;Parent=MsG0680034997.01.T01 |
| Gene Sequence |
| Protein sequence |
