Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035003.01.T01 | GAU37828.1 | 63.087 | 149 | 47 | 2 | 1 | 148 | 1 | 142 | 1.04E-46 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035003.01.T01 | A0A2Z6MZ06 | 63.087 | 149 | 47 | 2 | 1 | 148 | 1 | 142 | 4.94e-47 | 159 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005424.01 | MsG0680035003.01 | 0.805061 | 1.601188e-49 | 8.389519e-47 |
| MsG0380011614.01 | MsG0680035003.01 | 0.803498 | 3.387675e-49 | 1.705046e-46 |
| MsG0480023377.01 | MsG0680035003.01 | 0.802818 | 4.683140e-49 | 2.316520e-46 |
| MsG0580025799.01 | MsG0680035003.01 | 0.805623 | 1.220524e-49 | 6.488099e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035003.01.T01 | MTR_2g070580 | 84.416 | 77 | 8 | 1 | 68 | 144 | 15 | 87 | 4.04e-39 | 129 |
| MsG0680035003.01.T01 | MTR_8g019070 | 60.000 | 95 | 36 | 1 | 23 | 115 | 91 | 185 | 5.80e-28 | 103 |
| MsG0680035003.01.T01 | MTR_1g006970 | 65.789 | 76 | 8 | 1 | 92 | 167 | 1 | 58 | 2.07e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCAACTGATTTGGATATA+TGG | 0.220847 | 6:-97606711 | MsG0680035003.01.T01:CDS |
| GTATGAAATGCCAACTGATT+TGG | 0.307692 | 6:-97606719 | None:intergenic |
| TGCTCATTTGATTCACTGTT+GGG | 0.320236 | 6:+97606394 | None:intergenic |
| TGCTGCCGTTATGAAACATT+TGG | 0.344500 | 6:-97606449 | MsG0680035003.01.T01:CDS |
| ATGAAACATTTGGGATTTGT+TGG | 0.381336 | 6:-97606439 | MsG0680035003.01.T01:CDS |
| CACTGTTGGGGTTTGAAACA+TGG | 0.393708 | 6:+97606407 | None:intergenic |
| GCTGCCGTTATGAAACATTT+GGG | 0.418841 | 6:-97606448 | MsG0680035003.01.T01:CDS |
| GCCAACTGATTTGGATATAT+GGG | 0.422049 | 6:-97606710 | MsG0680035003.01.T01:CDS |
| TTGATTTGAAGATGGAATCA+AGG | 0.453518 | 6:+97606630 | None:intergenic |
| AAAGGGAGGATATTTGGTCT+TGG | 0.464262 | 6:-97606667 | MsG0680035003.01.T01:CDS |
| GTGCTCATTTGATTCACTGT+TGG | 0.469093 | 6:+97606393 | None:intergenic |
| TTTGTTTGTTGATTTGAAGA+TGG | 0.485071 | 6:+97606622 | None:intergenic |
| AACAAGAGAAGTTGCAGATG+AGG | 0.489038 | 6:-97606522 | MsG0680035003.01.T01:CDS |
| GATATATGGGTAGATTCTGT+TGG | 0.506068 | 6:-97606697 | MsG0680035003.01.T01:CDS |
| CAACATCTTCAGCAAAATCA+TGG | 0.507886 | 6:+97606320 | None:intergenic |
| CAACATCTTCAGCAAAATCA+TGG | 0.508374 | 6:+97606266 | None:intergenic |
| TGCTGAAGATGTTGATGAAG+AGG | 0.530294 | 6:-97606311 | MsG0680035003.01.T01:CDS |
| AAATGAGCACATTGATGAAG+AGG | 0.534323 | 6:-97606380 | MsG0680035003.01.T01:CDS |
| AAATCCCAAATGTTTCATAA+CGG | 0.566816 | 6:+97606444 | None:intergenic |
| ACCCATATATCCAAATCAGT+TGG | 0.567496 | 6:+97606709 | None:intergenic |
| ACTGTTGGGGTTTGAAACAT+GGG | 0.581536 | 6:+97606408 | None:intergenic |
| GCTCATTTGATTCACTGTTG+GGG | 0.595706 | 6:+97606395 | None:intergenic |
| TACACATGCTAATTCAGAAG+AGG | 0.601418 | 6:-97606599 | MsG0680035003.01.T01:CDS |
| AGATGAGGCAACAAATGCAA+CGG | 0.644760 | 6:-97606507 | MsG0680035003.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCCCAAATGTTTCATAA+CGG | + | Chr6:97606502-97606521 | None:intergenic | 25.0% |
| !! | ATTCTGTTGGTAGAAAAAAA+GGG | - | Chr6:97606259-97606278 | MsG0680035003.01.T01:CDS | 25.0% |
| !!! | TTTGTTTGTTGATTTGAAGA+TGG | + | Chr6:97606324-97606343 | None:intergenic | 25.0% |
| AGAAAAAAAGGGAGGATATT+TGG | - | Chr6:97606270-97606289 | MsG0680035003.01.T01:CDS | 30.0% | |
| ! | ATGAAACATTTGGGATTTGT+TGG | - | Chr6:97606504-97606523 | MsG0680035003.01.T01:CDS | 30.0% |
| ! | GATTCTGTTGGTAGAAAAAA+AGG | - | Chr6:97606258-97606277 | MsG0680035003.01.T01:CDS | 30.0% |
| ! | TTGATTTGAAGATGGAATCA+AGG | + | Chr6:97606316-97606335 | None:intergenic | 30.0% |
| AAATGAGCACATTGATGAAG+AGG | - | Chr6:97606563-97606582 | MsG0680035003.01.T01:CDS | 35.0% | |
| ACCCATATATCCAAATCAGT+TGG | + | Chr6:97606237-97606256 | None:intergenic | 35.0% | |
| CAACATCTTCAGCAAAATCA+TGG | + | Chr6:97606593-97606612 | None:intergenic | 35.0% | |
| GATATATGGGTAGATTCTGT+TGG | - | Chr6:97606246-97606265 | MsG0680035003.01.T01:CDS | 35.0% | |
| TACACATGCTAATTCAGAAG+AGG | - | Chr6:97606344-97606363 | MsG0680035003.01.T01:CDS | 35.0% | |
| TGCTCATTTGATTCACTGTT+GGG | + | Chr6:97606552-97606571 | None:intergenic | 35.0% | |
| !! | GCCAACTGATTTGGATATAT+GGG | - | Chr6:97606233-97606252 | MsG0680035003.01.T01:CDS | 35.0% |
| !! | TGCCAACTGATTTGGATATA+TGG | - | Chr6:97606232-97606251 | MsG0680035003.01.T01:CDS | 35.0% |
| AAAGGGAGGATATTTGGTCT+TGG | - | Chr6:97606276-97606295 | MsG0680035003.01.T01:CDS | 40.0% | |
| AACAAGAGAAGTTGCAGATG+AGG | - | Chr6:97606421-97606440 | MsG0680035003.01.T01:CDS | 40.0% | |
| AGATGAGGCAACAAATGCAA+CGG | - | Chr6:97606436-97606455 | MsG0680035003.01.T01:CDS | 40.0% | |
| GCTCATTTGATTCACTGTTG+GGG | + | Chr6:97606551-97606570 | None:intergenic | 40.0% | |
| GCTGCCGTTATGAAACATTT+GGG | - | Chr6:97606495-97606514 | MsG0680035003.01.T01:CDS | 40.0% | |
| GTGCTCATTTGATTCACTGT+TGG | + | Chr6:97606553-97606572 | None:intergenic | 40.0% | |
| TGCTGCCGTTATGAAACATT+TGG | - | Chr6:97606494-97606513 | MsG0680035003.01.T01:CDS | 40.0% | |
| ! | ACTGTTGGGGTTTGAAACAT+GGG | + | Chr6:97606538-97606557 | None:intergenic | 40.0% |
| ! | CTGTTGGTAGAAAAAAAGGG+AGG | - | Chr6:97606262-97606281 | MsG0680035003.01.T01:CDS | 40.0% |
| ! | TGCTGAAGATGTTGATGAAG+AGG | - | Chr6:97606599-97606618 | MsG0680035003.01.T01:CDS | 40.0% |
| ! | CACTGTTGGGGTTTGAAACA+TGG | + | Chr6:97606539-97606558 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 97606231 | 97606734 | 97606231 | ID=MsG0680035003.01;Name=MsG0680035003.01 |
| Chr6 | mRNA | 97606231 | 97606734 | 97606231 | ID=MsG0680035003.01.T01;Parent=MsG0680035003.01;Name=MsG0680035003.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|167 |
| Chr6 | exon | 97606231 | 97606734 | 97606231 | ID=MsG0680035003.01.T01:exon:13856;Parent=MsG0680035003.01.T01 |
| Chr6 | CDS | 97606231 | 97606734 | 97606231 | ID=MsG0680035003.01.T01:cds;Parent=MsG0680035003.01.T01 |
| Gene Sequence |
| Protein sequence |
