Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025799.01.T01 | XP_003629262.1 | 93.6 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 2.87E-77 | 244 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025799.01.T01 | Q8L7E9 | 80.8 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.27E-69 | 215 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025799.01.T01 | G7LB65 | 93.600 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 1.37e-77 | 244 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047912.01 | MsG0580025799.01 | 0.806909 | 6.543581e-50 | 3.594848e-47 |
| MsG0080048764.01 | MsG0580025799.01 | 0.811530 | 6.686592e-51 | 4.149193e-48 |
| MsG0180000157.01 | MsG0580025799.01 | 0.809331 | 1.994462e-50 | 1.167695e-47 |
| MsG0180000550.01 | MsG0580025799.01 | 0.810471 | 1.133739e-50 | 6.840141e-48 |
| MsG0180000669.01 | MsG0580025799.01 | 0.825001 | 5.945283e-54 | 5.352533e-51 |
| MsG0180000739.01 | MsG0580025799.01 | 0.807368 | 5.230641e-50 | 2.908021e-47 |
| MsG0180000822.01 | MsG0580025799.01 | 0.811154 | 8.069679e-51 | 4.957805e-48 |
| MsG0180001043.01 | MsG0580025799.01 | 0.810560 | 1.084622e-50 | 6.559273e-48 |
| MsG0180001921.01 | MsG0580025799.01 | 0.801043 | 1.084411e-48 | 5.127779e-46 |
| MsG0180002075.01 | MsG0580025799.01 | 0.813150 | 2.961156e-51 | 1.918547e-48 |
| MsG0180002750.01 | MsG0580025799.01 | -0.800444 | 1.436836e-48 | 6.691411e-46 |
| MsG0180002894.01 | MsG0580025799.01 | 0.825634 | 4.212956e-54 | 3.861544e-51 |
| MsG0180004388.01 | MsG0580025799.01 | 0.825825 | 3.795134e-54 | 3.497629e-51 |
| MsG0180004824.01 | MsG0580025799.01 | 0.806412 | 8.330574e-50 | 4.518544e-47 |
| MsG0180005233.01 | MsG0580025799.01 | 0.848810 | 4.833692e-60 | 8.978621e-57 |
| MsG0180005306.01 | MsG0580025799.01 | 0.809480 | 1.853214e-50 | 1.089289e-47 |
| MsG0180005944.01 | MsG0580025799.01 | 0.805879 | 1.078854e-49 | 5.772692e-47 |
| MsG0280011070.01 | MsG0580025799.01 | 0.811859 | 5.671018e-51 | 3.549598e-48 |
| MsG0280011175.01 | MsG0580025799.01 | 0.810870 | 9.296730e-51 | 5.669221e-48 |
| MsG0380012186.01 | MsG0580025799.01 | 0.827931 | 1.191173e-54 | 1.166589e-51 |
| MsG0480018606.01 | MsG0580025799.01 | 0.820792 | 5.687433e-53 | 4.544649e-50 |
| MsG0480018970.01 | MsG0580025799.01 | 0.814707 | 1.343557e-51 | 9.080693e-49 |
| MsG0480019795.01 | MsG0580025799.01 | 0.808826 | 2.559323e-50 | 1.478230e-47 |
| MsG0480020720.01 | MsG0580025799.01 | 0.848264 | 6.846476e-60 | 1.249332e-56 |
| MsG0480021418.01 | MsG0580025799.01 | 0.804653 | 1.948190e-49 | 1.010078e-46 |
| MsG0480022768.01 | MsG0580025799.01 | 0.802462 | 5.546099e-49 | 2.718441e-46 |
| MsG0480022986.01 | MsG0580025799.01 | 0.808000 | 3.839082e-50 | 2.169957e-47 |
| MsG0480023377.01 | MsG0580025799.01 | 0.810468 | 1.135516e-50 | 6.850275e-48 |
| MsG0480023707.01 | MsG0580025799.01 | 0.801342 | 9.418793e-49 | 4.487292e-46 |
| MsG0480023776.01 | MsG0580025799.01 | 0.800919 | 1.149799e-48 | 5.419764e-46 |
| MsG0580024114.01 | MsG0580025799.01 | 0.821093 | 4.848825e-53 | 3.907358e-50 |
| MsG0580024472.01 | MsG0580025799.01 | 0.805861 | 1.088100e-49 | 5.819777e-47 |
| MsG0580025008.01 | MsG0580025799.01 | 0.800598 | 1.336874e-48 | 6.250194e-46 |
| MsG0580025726.01 | MsG0580025799.01 | 0.803329 | 3.672585e-49 | 1.840330e-46 |
| MsG0580025799.01 | MsG0580028072.01 | 0.804805 | 1.810719e-49 | 9.425579e-47 |
| MsG0580025799.01 | MsG0580029594.01 | 0.827388 | 1.607897e-54 | 1.550233e-51 |
| MsG0580025799.01 | MsG0580029762.01 | 0.807208 | 5.655269e-50 | 3.131145e-47 |
| MsG0580025799.01 | MsG0580030204.01 | 0.802131 | 6.489127e-49 | 3.153916e-46 |
| MsG0580025799.01 | MsG0580030235.01 | 0.814787 | 1.289598e-51 | 8.734807e-49 |
| MsG0580025799.01 | MsG0680030685.01 | 0.813354 | 2.670371e-51 | 1.739944e-48 |
| MsG0580025799.01 | MsG0680031020.01 | 0.807205 | 5.662330e-50 | 3.134839e-47 |
| MsG0580025799.01 | MsG0680031262.01 | 0.804134 | 2.499746e-49 | 1.278834e-46 |
| MsG0580025799.01 | MsG0680034655.01 | 0.806743 | 7.094134e-50 | 3.880638e-47 |
| MsG0580025799.01 | MsG0680035003.01 | 0.805623 | 1.220524e-49 | 6.488099e-47 |
| MsG0580025799.01 | MsG0680035844.01 | 0.800310 | 1.530563e-48 | 7.103942e-46 |
| MsG0580025799.01 | MsG0780038495.01 | 0.842437 | 2.588185e-58 | 3.924581e-55 |
| MsG0580025799.01 | MsG0780040726.01 | 0.821942 | 3.088039e-53 | 2.548276e-50 |
| MsG0580025799.01 | MsG0780040861.01 | 0.802706 | 4.940015e-49 | 2.436476e-46 |
| MsG0580025799.01 | MsG0780041192.01 | 0.824404 | 8.219842e-54 | 7.273212e-51 |
| MsG0580025799.01 | MsG0780041441.01 | 0.825186 | 5.377042e-54 | 4.866415e-51 |
| MsG0580025799.01 | MsG0780041456.01 | 0.835599 | 1.527495e-56 | 1.876915e-53 |
| MsG0580025799.01 | MsG0780041507.01 | 0.835343 | 1.773297e-56 | 2.162541e-53 |
| MsG0580025799.01 | MsG0880042053.01 | 0.818929 | 1.516239e-52 | 1.150650e-49 |
| MsG0580025799.01 | MsG0880042611.01 | 0.810624 | 1.050696e-50 | 6.365000e-48 |
| MsG0580025799.01 | MsG0880043252.01 | 0.801066 | 1.073049e-48 | 5.076821e-46 |
| MsG0580025799.01 | MsG0880044486.01 | 0.824249 | 8.938915e-54 | 7.874698e-51 |
| MsG0580025799.01 | MsG0880046479.01 | 0.804307 | 2.300614e-49 | 1.182201e-46 |
| MsG0580025799.01 | MsG0880046731.01 | 0.808822 | 2.564133e-50 | 1.480875e-47 |
| MsG0380015779.01 | MsG0580025799.01 | 0.836097 | 1.142559e-56 | 1.425232e-53 |
| MsG0380016859.01 | MsG0580025799.01 | 0.822838 | 1.912418e-53 | 1.619043e-50 |
| MsG0380017604.01 | MsG0580025799.01 | 0.820810 | 5.634581e-53 | 4.504709e-50 |
| MsG0380017631.01 | MsG0580025799.01 | 0.821135 | 4.741496e-53 | 3.825294e-50 |
| MsG0380017680.01 | MsG0580025799.01 | 0.844482 | 7.357118e-59 | 1.189412e-55 |
| MsG0380017941.01 | MsG0580025799.01 | 0.806442 | 8.211714e-50 | 4.457520e-47 |
| MsG0280008613.01 | MsG0580025799.01 | 0.801663 | 8.096308e-49 | 3.888425e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025799.01.T01 | MTR_8g075150 | 93.600 | 125 | 8 | 0 | 1 | 125 | 1 | 125 | 3.47e-81 | 244 |
| MsG0580025799.01.T01 | MTR_5g022670 | 82.114 | 123 | 22 | 0 | 3 | 125 | 2 | 124 | 4.00e-71 | 218 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025799.01.T01 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.41e-70 | 215 |
| MsG0580025799.01.T01 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.41e-70 | 215 |
| MsG0580025799.01.T01 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.41e-70 | 215 |
| MsG0580025799.01.T01 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.41e-70 | 215 |
| MsG0580025799.01.T01 | AT4G35920 | 80.800 | 125 | 24 | 0 | 1 | 125 | 1 | 125 | 7.41e-70 | 215 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.20e-58 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 6.27e-58 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.03e-57 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.03e-57 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-57 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-57 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-57 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-57 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.16e-57 | 184 |
| MsG0580025799.01.T01 | AT2G17780 | 70.400 | 125 | 37 | 0 | 1 | 125 | 1 | 125 | 1.21e-57 | 184 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAGAGGCTCTCGGGTTTC+AGG | 0.140533 | 5:+24342717 | None:intergenic |
| CTGCATCTAAGCCTATTAAT+TGG | 0.182088 | 5:+24342877 | None:intergenic |
| TTTGAGAAGGTCTTCTATTT+TGG | 0.212238 | 5:-24342679 | MsG0580025799.01.T01:CDS |
| GGAACGTTGTTTATCAATTT+AGG | 0.249469 | 5:-24342608 | MsG0580025799.01.T01:CDS |
| GCAAATGTAGCCCAATTAAT+AGG | 0.272486 | 5:-24342888 | MsG0580025799.01.T01:CDS |
| GCATCTTCATGGGAACAATT+TGG | 0.278027 | 5:-24342918 | MsG0580025799.01.T01:CDS |
| GCACAACATCTTAAGTTGAT+TGG | 0.319124 | 5:-24342783 | MsG0580025799.01.T01:CDS |
| TAGATGCAGTAAAGTTGATT+AGG | 0.362979 | 5:-24342863 | MsG0580025799.01.T01:CDS |
| CACAACATCTTAAGTTGATT+GGG | 0.378407 | 5:-24342782 | MsG0580025799.01.T01:CDS |
| CTAGTTGTTCTAGAGGCTCT+CGG | 0.379192 | 5:+24342709 | None:intergenic |
| GATATAGAATGGCATCTTCA+TGG | 0.416346 | 5:-24342929 | None:intergenic |
| TGCATCTAAGCCTATTAATT+GGG | 0.419039 | 5:+24342878 | None:intergenic |
| CTAGAGGCTCTCGGGTTTCA+GGG | 0.434211 | 5:+24342718 | None:intergenic |
| CTATCTTTATTTGCTCGCCA+TGG | 0.439513 | 5:-24342634 | MsG0580025799.01.T01:CDS |
| TATCTTTATTTGCTCGCCAT+GGG | 0.488601 | 5:-24342633 | MsG0580025799.01.T01:CDS |
| TAGTTGTTCTAGAGGCTCTC+GGG | 0.496041 | 5:+24342710 | None:intergenic |
| TTTATTTGCTCGCCATGGGA+TGG | 0.519477 | 5:-24342629 | MsG0580025799.01.T01:CDS |
| ATATAGAATGGCATCTTCAT+GGG | 0.542232 | 5:-24342928 | None:intergenic |
| GCATCTTCTAGTTGTTCTAG+AGG | 0.556880 | 5:+24342702 | None:intergenic |
| CGTTGTTTATCAATTTAGGA+AGG | 0.558364 | 5:-24342604 | MsG0580025799.01.T01:CDS |
| ATAAACAACGTTCCATCCCA+TGG | 0.601527 | 5:+24342617 | None:intergenic |
| AACTAGAAGATGCTTTGAGA+AGG | 0.613004 | 5:-24342692 | MsG0580025799.01.T01:CDS |
| CGAAAGCAGCAAATACAGCA+AGG | 0.638455 | 5:-24342833 | MsG0580025799.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGTTGTTTATCAATTTAGGA+AGG | - | Chr5:24342880-24342899 | MsG0580025799.01.T01:CDS | 30.0% | |
| GGAACGTTGTTTATCAATTT+AGG | - | Chr5:24342876-24342895 | MsG0580025799.01.T01:CDS | 30.0% | |
| TGCATCTAAGCCTATTAATT+GGG | + | Chr5:24342609-24342628 | None:intergenic | 30.0% | |
| ! | TAGATGCAGTAAAGTTGATT+AGG | - | Chr5:24342621-24342640 | MsG0580025799.01.T01:CDS | 30.0% |
| ! | TTTGAGAAGGTCTTCTATTT+TGG | - | Chr5:24342805-24342824 | MsG0580025799.01.T01:CDS | 30.0% |
| !! | CACAACATCTTAAGTTGATT+GGG | - | Chr5:24342702-24342721 | MsG0580025799.01.T01:CDS | 30.0% |
| !!! | TATTTTGGTCAATAGTTGTC+AGG | - | Chr5:24342820-24342839 | MsG0580025799.01.T01:CDS | 30.0% |
| CTGCATCTAAGCCTATTAAT+TGG | + | Chr5:24342610-24342629 | None:intergenic | 35.0% | |
| GCAAATGTAGCCCAATTAAT+AGG | - | Chr5:24342596-24342615 | MsG0580025799.01.T01:CDS | 35.0% | |
| ! | TATCTTTATTTGCTCGCCAT+GGG | - | Chr5:24342851-24342870 | MsG0580025799.01.T01:CDS | 35.0% |
| !! | AACTAGAAGATGCTTTGAGA+AGG | - | Chr5:24342792-24342811 | MsG0580025799.01.T01:CDS | 35.0% |
| !! | GCACAACATCTTAAGTTGAT+TGG | - | Chr5:24342701-24342720 | MsG0580025799.01.T01:CDS | 35.0% |
| ATAAACAACGTTCCATCCCA+TGG | + | Chr5:24342870-24342889 | None:intergenic | 40.0% | |
| GCATCTTCATGGGAACAATT+TGG | - | Chr5:24342566-24342585 | MsG0580025799.01.T01:CDS | 40.0% | |
| ! | CTATCTTTATTTGCTCGCCA+TGG | - | Chr5:24342850-24342869 | MsG0580025799.01.T01:CDS | 40.0% |
| ! | GCATCTTCTAGTTGTTCTAG+AGG | + | Chr5:24342785-24342804 | None:intergenic | 40.0% |
| CGAAAGCAGCAAATACAGCA+AGG | - | Chr5:24342651-24342670 | MsG0580025799.01.T01:CDS | 45.0% | |
| TTTATTTGCTCGCCATGGGA+TGG | - | Chr5:24342855-24342874 | MsG0580025799.01.T01:CDS | 45.0% | |
| !! | CTAGTTGTTCTAGAGGCTCT+CGG | + | Chr5:24342778-24342797 | None:intergenic | 45.0% |
| !! | TAGTTGTTCTAGAGGCTCTC+GGG | + | Chr5:24342777-24342796 | None:intergenic | 45.0% |
| CTAGAGGCTCTCGGGTTTCA+GGG | + | Chr5:24342769-24342788 | None:intergenic | 55.0% | |
| TCTAGAGGCTCTCGGGTTTC+AGG | + | Chr5:24342770-24342789 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 24342563 | 24342943 | 24342563 | ID=MsG0580025799.01;Name=MsG0580025799.01 |
| Chr5 | mRNA | 24342563 | 24342943 | 24342563 | ID=MsG0580025799.01.T01;Parent=MsG0580025799.01;Name=MsG0580025799.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|126 |
| Chr5 | exon | 24342563 | 24342943 | 24342563 | ID=MsG0580025799.01.T01:exon:3901;Parent=MsG0580025799.01.T01 |
| Chr5 | CDS | 24342563 | 24342943 | 24342563 | ID=MsG0580025799.01.T01:cds;Parent=MsG0580025799.01.T01 |
| Gene Sequence |
| Protein sequence |
