Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035570.01.T01 | XP_013450947.1 | 63.889 | 108 | 26 | 2 | 6 | 113 | 74 | 168 | 7.52E-34 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035570.01.T01 | Q9LXG9 | 53.125 | 96 | 35 | 1 | 6 | 101 | 91 | 176 | 4.81E-23 | 93.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035570.01.T01 | A0A072U5P5 | 63.889 | 108 | 26 | 2 | 6 | 113 | 74 | 168 | 3.59e-34 | 128 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680030732.01 | MsG0680035570.01 | 0.896109 | 5.053432e-76 | 5.389467e-72 |
| MsG0680035570.01 | MsG0780036060.01 | 0.818370 | 2.030966e-52 | 1.517528e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035570.01.T01 | MTR_6g011530 | 63.889 | 108 | 26 | 2 | 6 | 113 | 74 | 168 | 9.11e-38 | 128 |
| MsG0680035570.01.T01 | MTR_7g009710 | 52.475 | 101 | 26 | 2 | 7 | 103 | 79 | 161 | 1.86e-24 | 94.4 |
| MsG0680035570.01.T01 | MTR_2g084225 | 41.739 | 115 | 34 | 5 | 6 | 106 | 35 | 130 | 9.72e-19 | 78.6 |
| MsG0680035570.01.T01 | MTR_4g080083 | 36.364 | 110 | 37 | 3 | 6 | 101 | 35 | 125 | 2.35e-16 | 71.6 |
| MsG0680035570.01.T01 | MTR_7g086380 | 45.000 | 100 | 50 | 1 | 3 | 97 | 58 | 157 | 3.25e-16 | 72.4 |
| MsG0680035570.01.T01 | MTR_4g080083 | 36.364 | 110 | 37 | 3 | 6 | 101 | 35 | 125 | 3.54e-16 | 71.6 |
| MsG0680035570.01.T01 | MTR_6g027730 | 86.486 | 37 | 5 | 0 | 61 | 97 | 42 | 78 | 4.27e-16 | 70.9 |
| MsG0680035570.01.T01 | MTR_2g058330 | 51.389 | 72 | 26 | 3 | 42 | 109 | 90 | 156 | 1.35e-14 | 67.8 |
| MsG0680035570.01.T01 | MTR_2g055320 | 51.389 | 72 | 26 | 3 | 42 | 109 | 81 | 147 | 1.40e-14 | 67.8 |
| MsG0680035570.01.T01 | MTR_2g016230 | 55.814 | 43 | 18 | 1 | 59 | 101 | 82 | 123 | 6.87e-13 | 62.8 |
| MsG0680035570.01.T01 | MTR_5g064550 | 58.974 | 39 | 16 | 0 | 59 | 97 | 89 | 127 | 2.75e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035570.01.T01 | AT5G15120 | 53.125 | 96 | 35 | 1 | 6 | 101 | 91 | 176 | 4.90e-24 | 93.6 |
| MsG0680035570.01.T01 | AT5G39890 | 49.038 | 104 | 30 | 2 | 6 | 104 | 80 | 165 | 7.71e-22 | 86.7 |
| MsG0680035570.01.T01 | AT5G39890 | 49.505 | 101 | 28 | 2 | 6 | 101 | 80 | 162 | 9.26e-22 | 87.0 |
| MsG0680035570.01.T01 | AT2G42670 | 58.929 | 56 | 21 | 1 | 59 | 112 | 84 | 139 | 3.20e-18 | 77.0 |
| MsG0680035570.01.T01 | AT2G42670 | 74.359 | 39 | 10 | 0 | 59 | 97 | 16 | 54 | 1.39e-17 | 73.9 |
| MsG0680035570.01.T01 | AT2G42670 | 74.359 | 39 | 10 | 0 | 59 | 97 | 84 | 122 | 1.67e-17 | 75.1 |
| MsG0680035570.01.T01 | AT3G58670 | 71.795 | 39 | 11 | 0 | 59 | 97 | 41 | 79 | 1.22e-16 | 72.4 |
| MsG0680035570.01.T01 | AT3G58670 | 71.795 | 39 | 11 | 0 | 59 | 97 | 84 | 122 | 1.40e-16 | 72.8 |
| MsG0680035570.01.T01 | AT3G58670 | 71.795 | 39 | 11 | 0 | 59 | 97 | 84 | 122 | 1.40e-16 | 72.8 |
| MsG0680035570.01.T01 | AT3G58670 | 71.795 | 39 | 11 | 0 | 59 | 97 | 84 | 122 | 1.40e-16 | 72.8 |
| MsG0680035570.01.T01 | AT1G18490 | 39.583 | 96 | 48 | 3 | 6 | 97 | 66 | 155 | 3.07e-14 | 67.0 |
Find 25 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCCTATATGCCATATTTC+AGG | 0.177781 | 6:-108602345 | MsG0680035570.01.T01:CDS |
| TTGAAAATGGTAGGAGATTC+AGG | 0.242628 | 6:+108601751 | None:intergenic |
| ACTTTCTGAGGGTTTGAAAA+TGG | 0.319904 | 6:+108601738 | None:intergenic |
| GGATCCAAGTCAACATCTTC+TGG | 0.329534 | 6:+108602363 | None:intergenic |
| TGGATCAATATCGGATGGTT+TGG | 0.359783 | 6:+108602100 | None:intergenic |
| TGCACATCAAATCATATGAT+TGG | 0.370445 | 6:-108601789 | MsG0680035570.01.T01:CDS |
| GATGCATTTGCCCTGAAATA+TGG | 0.421163 | 6:+108602334 | None:intergenic |
| TTATGAGTGTGAGAAATTCT+CGG | 0.469187 | 6:-108602278 | MsG0680035570.01.T01:intron |
| ATCCTATATGCCATATTTCA+GGG | 0.473301 | 6:-108602344 | MsG0680035570.01.T01:CDS |
| GGTAACAGTTGGATCAATAT+CGG | 0.475062 | 6:+108602091 | None:intergenic |
| TGCCCTGAAATATGGCATAT+AGG | 0.477270 | 6:+108602342 | None:intergenic |
| GCACATCAAATCATATGATT+GGG | 0.481019 | 6:-108601788 | MsG0680035570.01.T01:CDS |
| ATAGTACTACTCACTTTCTG+AGG | 0.535516 | 6:+108601726 | None:intergenic |
| TCTGAGGGTTTGAAAATGGT+AGG | 0.554115 | 6:+108601742 | None:intergenic |
| AAAACCAGAAGATGTTGACT+TGG | 0.573524 | 6:-108602367 | MsG0680035570.01.T01:CDS |
| TAGTACTACTCACTTTCTGA+GGG | 0.577095 | 6:+108601727 | None:intergenic |
| GGGCAAATGCATCTCACAGA+AGG | 0.579084 | 6:-108602324 | MsG0680035570.01.T01:CDS |
| ACAGTTGGATCAATATCGGA+TGG | 0.600830 | 6:+108602095 | None:intergenic |
| CATCAAATCATATGATTGGG+TGG | 0.621774 | 6:-108601785 | MsG0680035570.01.T01:CDS |
| TGTAGAGGAATAACACCAGA+AGG | 0.622496 | 6:+108601859 | None:intergenic |
| ATTCCAGGGTGATTATGTAG+AGG | 0.643712 | 6:+108601844 | None:intergenic |
| AGTCATACAACGGTAACAGT+TGG | 0.650771 | 6:+108602080 | None:intergenic |
| ATTCCTCTACATAATCACCC+TGG | 0.691462 | 6:-108601847 | MsG0680035570.01.T01:CDS |
| AGAGGAATAACACCAGAAGG+TGG | 0.695453 | 6:+108601862 | None:intergenic |
| AAAATGGTAGGAGATTCAGG+AGG | 0.774453 | 6:+108601754 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATGATGTTTAATAAGAAA+TGG | - | Chr6:108601896-108601915 | MsG0680035570.01.T01:intron | 15.0% |
| !! | AACACATTAAAATGTGTATA+AGG | + | Chr6:108602216-108602235 | None:intergenic | 20.0% |
| !!! | GGATTTTCTAAAAATAAATG+TGG | + | Chr6:108602004-108602023 | None:intergenic | 20.0% |
| ! | TCATATATAACAGTTTAGAC+TGG | + | Chr6:108601882-108601901 | None:intergenic | 25.0% |
| !! | ATGTGCAAGTATGTAATTTT+TGG | + | Chr6:108601824-108601843 | None:intergenic | 25.0% |
| !! | TATTTTGTGAACTGTTTGAT+CGG | + | Chr6:108602107-108602126 | None:intergenic | 25.0% |
| !! | TTTTAATGTGTTGTACAGAT+GGG | - | Chr6:108602224-108602243 | MsG0680035570.01.T01:intron | 25.0% |
| !!! | ATTTTAATGTGTTGTACAGA+TGG | - | Chr6:108602223-108602242 | MsG0680035570.01.T01:intron | 25.0% |
| !!! | GTTTTTAGTAAGCTACTTTT+TGG | - | Chr6:108602305-108602324 | MsG0680035570.01.T01:CDS | 25.0% |
| ATCCTATATGCCATATTTCA+GGG | - | Chr6:108601778-108601797 | MsG0680035570.01.T01:CDS | 30.0% | |
| ATTCATAATATCTCGACTCT+AGG | - | Chr6:108602071-108602090 | MsG0680035570.01.T01:intron | 30.0% | |
| GCACATCAAATCATATGATT+GGG | - | Chr6:108602334-108602353 | MsG0680035570.01.T01:CDS | 30.0% | |
| TACTAAAAACTGTCATTCCA+GGG | + | Chr6:108602295-108602314 | None:intergenic | 30.0% | |
| TGCACATCAAATCATATGAT+TGG | - | Chr6:108602333-108602352 | MsG0680035570.01.T01:CDS | 30.0% | |
| TTACTAAAAACTGTCATTCC+AGG | + | Chr6:108602296-108602315 | None:intergenic | 30.0% | |
| TTATGAGTGTGAGAAATTCT+CGG | - | Chr6:108601844-108601863 | MsG0680035570.01.T01:CDS | 30.0% | |
| TTCATAATATCTCGACTCTA+GGG | - | Chr6:108602072-108602091 | MsG0680035570.01.T01:intron | 30.0% | |
| CATCAAATCATATGATTGGG+TGG | - | Chr6:108602337-108602356 | MsG0680035570.01.T01:CDS | 35.0% | |
| GAATTCATGCAGTCATACAA+CGG | + | Chr6:108602055-108602074 | None:intergenic | 35.0% | |
| GATCCTATATGCCATATTTC+AGG | - | Chr6:108601777-108601796 | MsG0680035570.01.T01:CDS | 35.0% | |
| GGTAACAGTTGGATCAATAT+CGG | + | Chr6:108602034-108602053 | None:intergenic | 35.0% | |
| TTGAAAATGGTAGGAGATTC+AGG | + | Chr6:108602374-108602393 | None:intergenic | 35.0% | |
| ! | AAAACCAGAAGATGTTGACT+TGG | - | Chr6:108601755-108601774 | MsG0680035570.01.T01:CDS | 35.0% |
| ! | ACTTTCTGAGGGTTTGAAAA+TGG | + | Chr6:108602387-108602406 | None:intergenic | 35.0% |
| !! | ATATTTTGTTTGCCACCTTC+TGG | - | Chr6:108602248-108602267 | MsG0680035570.01.T01:intron | 35.0% |
| AAAATGGTAGGAGATTCAGG+AGG | + | Chr6:108602371-108602390 | None:intergenic | 40.0% | |
| ACAGTTGGATCAATATCGGA+TGG | + | Chr6:108602030-108602049 | None:intergenic | 40.0% | |
| AGTCATACAACGGTAACAGT+TGG | + | Chr6:108602045-108602064 | None:intergenic | 40.0% | |
| ATTCCAGGGTGATTATGTAG+AGG | + | Chr6:108602281-108602300 | None:intergenic | 40.0% | |
| ATTCCTCTACATAATCACCC+TGG | - | Chr6:108602275-108602294 | MsG0680035570.01.T01:intron | 40.0% | |
| CATTCACACAGTCAATGCAT+TGG | - | Chr6:108601944-108601963 | MsG0680035570.01.T01:intron | 40.0% | |
| GAAGAGATATCCGAAGTGAA+GGG | + | Chr6:108601925-108601944 | None:intergenic | 40.0% | |
| GATGCATTTGCCCTGAAATA+TGG | + | Chr6:108601791-108601810 | None:intergenic | 40.0% | |
| TCTGAGGGTTTGAAAATGGT+AGG | + | Chr6:108602383-108602402 | None:intergenic | 40.0% | |
| TGAAGAGATATCCGAAGTGA+AGG | + | Chr6:108601926-108601945 | None:intergenic | 40.0% | |
| TGCCCTGAAATATGGCATAT+AGG | + | Chr6:108601783-108601802 | None:intergenic | 40.0% | |
| TGGATCAATATCGGATGGTT+TGG | + | Chr6:108602025-108602044 | None:intergenic | 40.0% | |
| TGTAGAGGAATAACACCAGA+AGG | + | Chr6:108602266-108602285 | None:intergenic | 40.0% | |
| ! | GAAATGGATTCCCTTCACTT+CGG | - | Chr6:108601912-108601931 | MsG0680035570.01.T01:intron | 40.0% |
| AGAGGAATAACACCAGAAGG+TGG | + | Chr6:108602263-108602282 | None:intergenic | 45.0% | |
| GGATCCAAGTCAACATCTTC+TGG | + | Chr6:108601762-108601781 | None:intergenic | 45.0% | |
| ! | ACTGTTCGATCTGAAACCAG+CGG | + | Chr6:108601973-108601992 | None:intergenic | 45.0% |
| GGGCAAATGCATCTCACAGA+AGG | - | Chr6:108601798-108601817 | MsG0680035570.01.T01:CDS | 50.0% | |
| ! | GTCAATGCATTGGCGACCGC+TGG | - | Chr6:108601954-108601973 | MsG0680035570.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 108601732 | 108602412 | 108601732 | ID=MsG0680035570.01;Name=MsG0680035570.01 |
| Chr6 | mRNA | 108601732 | 108602412 | 108601732 | ID=MsG0680035570.01.T01;Parent=MsG0680035570.01;Name=MsG0680035570.01.T01;_AED=0.46;_eAED=0.53;_QI=0|0|0|1|0|0.66|3|0|114 |
| Chr6 | exon | 108602279 | 108602412 | 108602279 | ID=MsG0680035570.01.T01:exon:1172;Parent=MsG0680035570.01.T01 |
| Chr6 | exon | 108602088 | 108602127 | 108602088 | ID=MsG0680035570.01.T01:exon:1173;Parent=MsG0680035570.01.T01 |
| Chr6 | exon | 108601732 | 108601902 | 108601732 | ID=MsG0680035570.01.T01:exon:1174;Parent=MsG0680035570.01.T01 |
| Chr6 | CDS | 108602279 | 108602412 | 108602279 | ID=MsG0680035570.01.T01:cds;Parent=MsG0680035570.01.T01 |
| Chr6 | CDS | 108602088 | 108602127 | 108602088 | ID=MsG0680035570.01.T01:cds;Parent=MsG0680035570.01.T01 |
| Chr6 | CDS | 108601732 | 108601902 | 108601732 | ID=MsG0680035570.01.T01:cds;Parent=MsG0680035570.01.T01 |
| Gene Sequence |
| Protein sequence |
