Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035714.01.T01 | KEH27158.1 | 46.939 | 196 | 59 | 3 | 1 | 153 | 1 | 194 | 8.98E-44 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035714.01.T01 | G8A0M6 | 46.939 | 196 | 59 | 3 | 1 | 153 | 1 | 194 | 4.29e-44 | 155 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480018653.01 | MsG0680035714.01 | 0.814897 | 1.219343e-51 | 8.283132e-49 |
| MsG0480023251.01 | MsG0680035714.01 | 0.807577 | 4.723509e-50 | 2.640279e-47 |
| MsG0680035714.01 | MsG0880043941.01 | 0.802028 | 6.814524e-49 | 3.303346e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035714.01.T01 | MTR_6g488260 | 46.939 | 196 | 59 | 3 | 1 | 153 | 1 | 194 | 1.09e-47 | 155 |
| MsG0680035714.01.T01 | MTR_6g488190 | 33.594 | 128 | 74 | 4 | 35 | 153 | 53 | 178 | 2.72e-12 | 63.5 |
| MsG0680035714.01.T01 | MTR_6g488200 | 45.652 | 92 | 41 | 5 | 68 | 153 | 125 | 213 | 5.82e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGTCGCGGATTCTTTA+AGG | 0.149783 | 6:-111157027 | MsG0680035714.01.T01:CDS |
| AAATAATATGTTATTTGATA+TGG | 0.194576 | 6:+111156827 | None:intergenic |
| AGTGGAAATAAAATAGATAT+TGG | 0.266231 | 6:-111156873 | MsG0680035714.01.T01:CDS |
| GATATTAAGGACAAGAGTTT+AGG | 0.308639 | 6:-111157282 | MsG0680035714.01.T01:CDS |
| AATAGATATTGGACTTTCAA+TGG | 0.360085 | 6:-111156862 | MsG0680035714.01.T01:CDS |
| TTCTTTCATGGAGGAGGAAG+GGG | 0.389696 | 6:-111156993 | MsG0680035714.01.T01:CDS |
| AAAGAATCCGCGACTTTCAT+AGG | 0.395410 | 6:+111157031 | None:intergenic |
| TGTGCCACAGTAACTTTGTA+AGG | 0.430676 | 6:+111156927 | None:intergenic |
| CAGAATCATGCTCATTGAAC+AGG | 0.443890 | 6:+111156789 | None:intergenic |
| AGCTAAAGCTGAAAATGATC+TGG | 0.457923 | 6:-111157102 | MsG0680035714.01.T01:CDS |
| AGTAATAGGAATATAAAACT+TGG | 0.512225 | 6:-111157336 | MsG0680035714.01.T01:CDS |
| ATATTATTTGTTTCAGCGGA+GGG | 0.515871 | 6:-111156813 | MsG0680035714.01.T01:CDS |
| TAACATATTATTTGTTTCAG+CGG | 0.536777 | 6:-111156817 | MsG0680035714.01.T01:CDS |
| TTTCTTTCATGGAGGAGGAA+GGG | 0.543712 | 6:-111156994 | MsG0680035714.01.T01:CDS |
| CATATTATTTGTTTCAGCGG+AGG | 0.560909 | 6:-111156814 | MsG0680035714.01.T01:CDS |
| GGACAAGAGTTTAGGAAAGA+CGG | 0.564396 | 6:-111157274 | MsG0680035714.01.T01:CDS |
| TAGGTCACGTTATCAGAAAG+CGG | 0.661044 | 6:+111157050 | None:intergenic |
| GAATCCTTACAAAGTTACTG+TGG | 0.689365 | 6:-111156931 | MsG0680035714.01.T01:CDS |
| GAACAGAATAGCATGCAGAG+AGG | 0.743502 | 6:-111156957 | MsG0680035714.01.T01:CDS |
| AACGTGACCTATGAAAGTCG+CGG | 0.766478 | 6:-111157038 | MsG0680035714.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATATGTTATTTGATA+TGG | + | Chr6:111157330-111157349 | None:intergenic | 10.0% |
| !! | AGTAATAGGAATATAAAACT+TGG | - | Chr6:111156818-111156837 | MsG0680035714.01.T01:CDS | 20.0% |
| !! | AGTGGAAATAAAATAGATAT+TGG | - | Chr6:111157281-111157300 | MsG0680035714.01.T01:CDS | 20.0% |
| !! | ATTCCAAAAAAGAGATATTA+AGG | - | Chr6:111156859-111156878 | MsG0680035714.01.T01:CDS | 20.0% |
| !! | TAACATATTATTTGTTTCAG+CGG | - | Chr6:111157337-111157356 | MsG0680035714.01.T01:CDS | 20.0% |
| !!! | GAAACAAATTTTTTCTTTCA+TGG | - | Chr6:111157149-111157168 | MsG0680035714.01.T01:intron | 20.0% |
| ! | AATAGATATTGGACTTTCAA+TGG | - | Chr6:111157292-111157311 | MsG0680035714.01.T01:CDS | 25.0% |
| ! | TATATCTGCTATCATTGAAA+TGG | + | Chr6:111157008-111157027 | None:intergenic | 25.0% |
| ! | TCTATCAAATACTCTAGTTA+AGG | + | Chr6:111156959-111156978 | None:intergenic | 25.0% |
| !! | CCACTAAAATCATTTTCATT+GGG | + | Chr6:111157266-111157285 | None:intergenic | 25.0% |
| !! | TCCACTAAAATCATTTTCAT+TGG | + | Chr6:111157267-111157286 | None:intergenic | 25.0% |
| !!! | ACAAATTTTTTCTTTCATGG+AGG | - | Chr6:111157152-111157171 | MsG0680035714.01.T01:intron | 25.0% |
| !!! | ATCTCTTTTTTGGAATTTCA+TGG | + | Chr6:111156855-111156874 | None:intergenic | 25.0% |
| !!! | GAAATAGTTTTGCTAGTAAT+AGG | - | Chr6:111156804-111156823 | MsG0680035714.01.T01:CDS | 25.0% |
| !!! | TGTCCTTAATATCTCTTTTT+TGG | + | Chr6:111156865-111156884 | None:intergenic | 25.0% |
| AAGTGAAATACATCATTGAC+GGG | - | Chr6:111156917-111156936 | MsG0680035714.01.T01:CDS | 30.0% | |
| ATATTATTTGTTTCAGCGGA+GGG | - | Chr6:111157341-111157360 | MsG0680035714.01.T01:CDS | 30.0% | |
| GAAGTGAAATACATCATTGA+CGG | - | Chr6:111156916-111156935 | MsG0680035714.01.T01:CDS | 30.0% | |
| GATATTAAGGACAAGAGTTT+AGG | - | Chr6:111156872-111156891 | MsG0680035714.01.T01:CDS | 30.0% | |
| ! | ACTAGAGTATTTGATAGAGA+TGG | - | Chr6:111156961-111156980 | MsG0680035714.01.T01:CDS | 30.0% |
| ! | CCCAATGAAAATGATTTTAG+TGG | - | Chr6:111157263-111157282 | MsG0680035714.01.T01:CDS | 30.0% |
| !!! | AATTTTTTCTTTCATGGAGG+AGG | - | Chr6:111157155-111157174 | MsG0680035714.01.T01:intron | 30.0% |
| AGCTAAAGCTGAAAATGATC+TGG | - | Chr6:111157052-111157071 | MsG0680035714.01.T01:CDS | 35.0% | |
| CATATTATTTGTTTCAGCGG+AGG | - | Chr6:111157340-111157359 | MsG0680035714.01.T01:CDS | 35.0% | |
| GAATCCTTACAAAGTTACTG+TGG | - | Chr6:111157223-111157242 | MsG0680035714.01.T01:intron | 35.0% | |
| AAAGAATCCGCGACTTTCAT+AGG | + | Chr6:111157126-111157145 | None:intergenic | 40.0% | |
| GGACAAGAGTTTAGGAAAGA+CGG | - | Chr6:111156880-111156899 | MsG0680035714.01.T01:CDS | 40.0% | |
| TAGGTCACGTTATCAGAAAG+CGG | + | Chr6:111157107-111157126 | None:intergenic | 40.0% | |
| TGAAAGTCGCGGATTCTTTA+AGG | - | Chr6:111157127-111157146 | MsG0680035714.01.T01:CDS | 40.0% | |
| TGTGCCACAGTAACTTTGTA+AGG | + | Chr6:111157230-111157249 | None:intergenic | 40.0% | |
| TTTCTTTCATGGAGGAGGAA+GGG | - | Chr6:111157160-111157179 | MsG0680035714.01.T01:intron | 40.0% | |
| ! | TTTTCTTTCATGGAGGAGGA+AGG | - | Chr6:111157159-111157178 | MsG0680035714.01.T01:intron | 40.0% |
| AACGTGACCTATGAAAGTCG+CGG | - | Chr6:111157116-111157135 | MsG0680035714.01.T01:CDS | 45.0% | |
| GAACAGAATAGCATGCAGAG+AGG | - | Chr6:111157197-111157216 | MsG0680035714.01.T01:intron | 45.0% | |
| GATAGAGATGGTAGCACTCA+AGG | - | Chr6:111156973-111156992 | MsG0680035714.01.T01:CDS | 45.0% | |
| TTCTTTCATGGAGGAGGAAG+GGG | - | Chr6:111157161-111157180 | MsG0680035714.01.T01:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 111156800 | 111157376 | 111156800 | ID=MsG0680035714.01;Name=MsG0680035714.01 |
| Chr6 | mRNA | 111156800 | 111157376 | 111156800 | ID=MsG0680035714.01.T01;Parent=MsG0680035714.01;Name=MsG0680035714.01.T01;_AED=0.50;_eAED=0.64;_QI=0|0|0|1|0|0|2|0|153 |
| Chr6 | exon | 111157263 | 111157376 | 111157263 | ID=MsG0680035714.01.T01:exon:15015;Parent=MsG0680035714.01.T01 |
| Chr6 | exon | 111156800 | 111157147 | 111156800 | ID=MsG0680035714.01.T01:exon:15014;Parent=MsG0680035714.01.T01 |
| Chr6 | CDS | 111157263 | 111157376 | 111157263 | ID=MsG0680035714.01.T01:cds;Parent=MsG0680035714.01.T01 |
| Chr6 | CDS | 111156800 | 111157147 | 111156800 | ID=MsG0680035714.01.T01:cds;Parent=MsG0680035714.01.T01 |
| Gene Sequence |
| Protein sequence |
