Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035997.01.T02 | XP_003621056.2 | 90.625 | 64 | 6 | 0 | 1 | 64 | 6 | 69 | 4.32E-33 | 122 |
| MsG0780035997.01.T01 | XP_003621056.2 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 6.08E-37 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035997.01.T01 | Q9SXZ2 | 49.275 | 69 | 34 | 1 | 1 | 69 | 1 | 68 | 1.19E-16 | 72.8 |
| MsG0780035997.01.T02 | Q9SXZ2 | 50.794 | 63 | 30 | 1 | 2 | 64 | 7 | 68 | 4.54E-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035997.01.T01 | F8T7T2 | 91.304 | 69 | 6 | 0 | 1 | 69 | 1 | 69 | 2.91e-37 | 132 |
| MsG0780035997.01.T02 | F8T7T2 | 90.625 | 64 | 6 | 0 | 1 | 64 | 6 | 69 | 2.06e-33 | 122 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580024908.01 | MsG0780035997.01 | 0.874668 | 5.639657e-68 | 2.560195e-64 |
| MsG0380016059.01 | MsG0780035997.01 | 0.818356 | 2.045401e-52 | 1.527715e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035997.01.T02 | MTR_7g006630 | 90.625 | 64 | 6 | 0 | 1 | 64 | 1 | 64 | 5.30e-37 | 121 |
| MsG0780035997.01.T02 | MTR_7g006690 | 82.540 | 63 | 11 | 0 | 2 | 64 | 7 | 69 | 2.13e-31 | 107 |
| MsG0780035997.01.T02 | MTR_6g033040 | 60.317 | 63 | 24 | 1 | 2 | 64 | 7 | 68 | 1.06e-18 | 75.1 |
| MsG0780035997.01.T02 | MTR_7g084970 | 60.317 | 63 | 24 | 1 | 2 | 64 | 7 | 68 | 1.88e-18 | 74.3 |
| MsG0780035997.01.T02 | MTR_7g085040 | 61.404 | 57 | 21 | 1 | 8 | 64 | 10 | 65 | 2.03e-17 | 71.6 |
| MsG0780035997.01.T02 | MTR_7g085020 | 53.968 | 63 | 28 | 1 | 2 | 64 | 8 | 69 | 1.01e-14 | 64.7 |
| MsG0780035997.01.T02 | MTR_1g060190 | 46.032 | 63 | 33 | 1 | 2 | 64 | 7 | 68 | 8.58e-13 | 59.7 |
| MsG0780035997.01.T02 | MTR_2g086270 | 41.270 | 63 | 36 | 1 | 2 | 64 | 6 | 67 | 4.59e-12 | 57.8 |
| MsG0780035997.01.T02 | MTR_7g104460 | 46.032 | 63 | 33 | 1 | 2 | 64 | 7 | 68 | 5.58e-12 | 57.8 |
| MsG0780035997.01.T02 | MTR_0020s0120 | 46.875 | 64 | 33 | 1 | 1 | 64 | 5 | 67 | 6.80e-12 | 57.4 |
| MsG0780035997.01.T02 | MTR_0020s0120 | 46.875 | 64 | 33 | 1 | 1 | 64 | 5 | 67 | 7.83e-12 | 57.4 |
| MsG0780035997.01.T01 | MTR_7g006630 | 90.625 | 64 | 6 | 0 | 6 | 69 | 1 | 64 | 3.91e-37 | 122 |
| MsG0780035997.01.T01 | MTR_7g006690 | 82.609 | 69 | 12 | 0 | 1 | 69 | 1 | 69 | 4.42e-35 | 117 |
| MsG0780035997.01.T01 | MTR_6g033040 | 57.971 | 69 | 28 | 1 | 1 | 69 | 1 | 68 | 1.79e-19 | 77.4 |
| MsG0780035997.01.T01 | MTR_7g084970 | 57.971 | 69 | 28 | 1 | 1 | 69 | 1 | 68 | 3.76e-19 | 76.6 |
| MsG0780035997.01.T01 | MTR_7g085040 | 61.404 | 57 | 21 | 1 | 13 | 69 | 10 | 65 | 2.08e-17 | 72.0 |
| MsG0780035997.01.T01 | MTR_7g085020 | 53.968 | 63 | 28 | 1 | 7 | 69 | 8 | 69 | 7.73e-15 | 65.5 |
| MsG0780035997.01.T01 | MTR_1g060190 | 46.032 | 63 | 33 | 1 | 7 | 69 | 7 | 68 | 9.65e-13 | 60.1 |
| MsG0780035997.01.T01 | MTR_7g104460 | 46.154 | 65 | 34 | 1 | 5 | 69 | 5 | 68 | 1.87e-12 | 59.3 |
| MsG0780035997.01.T01 | MTR_7g025450 | 83.871 | 31 | 5 | 0 | 1 | 31 | 1 | 31 | 2.29e-12 | 55.8 |
| MsG0780035997.01.T01 | MTR_0020s0120 | 45.455 | 66 | 35 | 1 | 4 | 69 | 3 | 67 | 4.78e-12 | 57.8 |
| MsG0780035997.01.T01 | MTR_0020s0120 | 45.455 | 66 | 35 | 1 | 4 | 69 | 3 | 67 | 5.24e-12 | 57.8 |
| MsG0780035997.01.T01 | MTR_2g086270 | 41.270 | 63 | 36 | 1 | 7 | 69 | 6 | 67 | 5.27e-12 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780035997.01.T02 | AT1G65480 | 50.794 | 63 | 30 | 1 | 2 | 64 | 7 | 68 | 4.63e-17 | 70.9 |
| MsG0780035997.01.T02 | AT1G65480 | 50.794 | 63 | 30 | 1 | 2 | 64 | 51 | 112 | 7.90e-17 | 71.2 |
| MsG0780035997.01.T02 | AT4G20370 | 52.381 | 63 | 29 | 1 | 2 | 64 | 7 | 68 | 2.03e-16 | 69.3 |
| MsG0780035997.01.T02 | AT5G62040 | 48.438 | 64 | 33 | 0 | 1 | 64 | 5 | 68 | 4.99e-15 | 65.9 |
| MsG0780035997.01.T02 | AT5G03840 | 43.750 | 64 | 35 | 1 | 1 | 64 | 9 | 71 | 3.52e-12 | 58.2 |
| MsG0780035997.01.T01 | AT1G65480 | 49.275 | 69 | 34 | 1 | 1 | 69 | 1 | 68 | 1.22e-17 | 72.8 |
| MsG0780035997.01.T01 | AT1G65480 | 49.275 | 69 | 34 | 1 | 1 | 69 | 45 | 112 | 2.60e-17 | 72.8 |
| MsG0780035997.01.T01 | AT4G20370 | 49.275 | 69 | 34 | 1 | 1 | 69 | 1 | 68 | 3.37e-17 | 71.6 |
| MsG0780035997.01.T01 | AT5G62040 | 46.970 | 66 | 35 | 0 | 4 | 69 | 3 | 68 | 1.98e-15 | 67.0 |
| MsG0780035997.01.T01 | AT5G03840 | 42.029 | 69 | 39 | 1 | 1 | 69 | 4 | 71 | 1.91e-12 | 59.3 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTCGGTGGATTGACTATT+TGG | 0.141339 | 7:+1099851 | None:intergenic |
| TGGTGCAATTGGAGATGTTT+TGG | 0.311297 | 7:-1099956 | MsG0780035997.01.T01:CDS |
| CTTGTGGTTTGTGGTGCAAT+TGG | 0.346962 | 7:-1099967 | MsG0780035997.01.T01:CDS |
| TTACAAATTCAGTGTCTTTG+AGG | 0.412870 | 7:-1099927 | MsG0780035997.01.T01:CDS |
| ATGAATCCTCTTGTGGTTTG+TGG | 0.422228 | 7:-1099976 | MsG0780035997.01.T01:CDS |
| AGAACTCTATACATTCTGAT+TGG | 0.422376 | 7:-1099805 | MsG0780035997.01.T01:CDS |
| TACAAATTCAGTGTCTTTGA+GGG | 0.441798 | 7:-1099926 | MsG0780035997.01.T01:CDS |
| TCTCGGTGGATTGACTATTT+GGG | 0.445444 | 7:+1099852 | None:intergenic |
| AAAGACACTGAATTTGTAAA+GGG | 0.449639 | 7:+1099931 | None:intergenic |
| CAAAGACACTGAATTTGTAA+AGG | 0.477373 | 7:+1099930 | None:intergenic |
| TTTCCACCAATTTGAACTCT+CGG | 0.485178 | 7:+1099835 | None:intergenic |
| AATCCACCGAGAGTTCAAAT+TGG | 0.489100 | 7:-1099841 | MsG0780035997.01.T01:CDS |
| CCACCGAGAGTTCAAATTGG+TGG | 0.512003 | 7:-1099838 | MsG0780035997.01.T01:CDS |
| TAAATCAATGAATCCTCTTG+TGG | 0.519287 | 7:-1099983 | MsG0780035997.01.T01:CDS |
| CGGTGGATTGACTATTTGGG+AGG | 0.527621 | 7:+1099855 | None:intergenic |
| GGTGGATTGACTATTTGGGA+GGG | 0.576305 | 7:+1099856 | None:intergenic |
| AACAAAGAAGTCAGCAACAG+TGG | 0.606254 | 7:-1099889 | MsG0780035997.01.T01:CDS |
| ATTGCACCACAAACCACAAG+AGG | 0.608864 | 7:+1099970 | None:intergenic |
| CCACCAATTTGAACTCTCGG+TGG | 0.629272 | 7:+1099838 | None:intergenic |
| TCAGAATGTATAGAGTTCTG+AGG | 0.684775 | 7:+1099809 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGACACTGAATTTGTAAA+GGG | + | Chr7:1099852-1099871 | None:intergenic | 25.0% |
| AGAACTCTATACATTCTGAT+TGG | - | Chr7:1099975-1099994 | MsG0780035997.01.T01:CDS | 30.0% | |
| CAAAGACACTGAATTTGTAA+AGG | + | Chr7:1099853-1099872 | None:intergenic | 30.0% | |
| TAAATCAATGAATCCTCTTG+TGG | - | Chr7:1099797-1099816 | MsG0780035997.01.T01:CDS | 30.0% | |
| TACAAATTCAGTGTCTTTGA+GGG | - | Chr7:1099854-1099873 | MsG0780035997.01.T01:CDS | 30.0% | |
| TTACAAATTCAGTGTCTTTG+AGG | - | Chr7:1099853-1099872 | MsG0780035997.01.T01:CDS | 30.0% | |
| TCAGAATGTATAGAGTTCTG+AGG | + | Chr7:1099974-1099993 | None:intergenic | 35.0% | |
| TTTCCACCAATTTGAACTCT+CGG | + | Chr7:1099948-1099967 | None:intergenic | 35.0% | |
| AACAAAGAAGTCAGCAACAG+TGG | - | Chr7:1099891-1099910 | MsG0780035997.01.T01:CDS | 40.0% | |
| AATCCACCGAGAGTTCAAAT+TGG | - | Chr7:1099939-1099958 | MsG0780035997.01.T01:CDS | 40.0% | |
| ATGAATCCTCTTGTGGTTTG+TGG | - | Chr7:1099804-1099823 | MsG0780035997.01.T01:CDS | 40.0% | |
| ! | TGGTGCAATTGGAGATGTTT+TGG | - | Chr7:1099824-1099843 | MsG0780035997.01.T01:CDS | 40.0% |
| !! | TCTCGGTGGATTGACTATTT+GGG | + | Chr7:1099931-1099950 | None:intergenic | 40.0% |
| ATTGCACCACAAACCACAAG+AGG | + | Chr7:1099813-1099832 | None:intergenic | 45.0% | |
| ! | CTTGTGGTTTGTGGTGCAAT+TGG | - | Chr7:1099813-1099832 | MsG0780035997.01.T01:CDS | 45.0% |
| !! | CTCTCGGTGGATTGACTATT+TGG | + | Chr7:1099932-1099951 | None:intergenic | 45.0% |
| !! | GGTGGATTGACTATTTGGGA+GGG | + | Chr7:1099927-1099946 | None:intergenic | 45.0% |
| CCACCAATTTGAACTCTCGG+TGG | + | Chr7:1099945-1099964 | None:intergenic | 50.0% | |
| CCACCGAGAGTTCAAATTGG+TGG | - | Chr7:1099942-1099961 | MsG0780035997.01.T01:CDS | 50.0% | |
| !! | CGGTGGATTGACTATTTGGG+AGG | + | Chr7:1099928-1099947 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 1099789 | 1100013 | 1099789 | ID=MsG0780035997.01;Name=MsG0780035997.01 |
| Chr7 | mRNA | 1099789 | 1100013 | 1099789 | ID=MsG0780035997.01.T01;Parent=MsG0780035997.01;Name=MsG0780035997.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|0|1|0|74 |
| Chr7 | exon | 1099789 | 1100013 | 1099789 | ID=MsG0780035997.01.T01:exon:607;Parent=MsG0780035997.01.T01 |
| Chr7 | CDS | 1099789 | 1100013 | 1099789 | ID=MsG0780035997.01.T01:cds;Parent=MsG0780035997.01.T01 |
| Chr7 | mRNA | 1099789 | 1099998 | 1099789 | ID=MsG0780035997.01.T02;Parent=MsG0780035997.01;Name=MsG0780035997.01.T02;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|0|1|0|69 |
| Chr7 | exon | 1099789 | 1099998 | 1099789 | ID=MsG0780035997.01.T02:exon:608;Parent=MsG0780035997.01.T02 |
| Chr7 | CDS | 1099789 | 1099998 | 1099789 | ID=MsG0780035997.01.T02:cds;Parent=MsG0780035997.01.T02 |
| Gene Sequence |
| Protein sequence |
