Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038322.01.T01 | XP_003622717.1 | 98.052 | 154 | 3 | 0 | 1 | 154 | 1 | 154 | 8.54E-107 | 311 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038322.01.T01 | G7KWY5 | 98.052 | 154 | 3 | 0 | 1 | 154 | 1 | 154 | 4.08e-107 | 311 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005881.01 | MsG0780038322.01 | 0.824119 | 9.590631e-54 | 8.417932e-51 |
| MsG0380017665.01 | MsG0780038322.01 | 0.816267 | 6.040338e-52 | 4.260242e-49 |
| MsG0780038322.01 | MsG0780041015.01 | 0.837068 | 6.464645e-57 | 8.302555e-54 |
| MsG0780038322.01 | MsG0880045707.01 | 0.832192 | 1.087302e-55 | 1.206431e-52 |
| MsG0780038322.01 | MsG0880047371.01 | 0.827263 | 1.723117e-54 | 1.655136e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038322.01.T01 | MTR_7g051230 | 98.052 | 154 | 3 | 0 | 1 | 154 | 1 | 154 | 1.03e-110 | 311 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038322.01.T01 | AT3G07480 | 63.057 | 157 | 46 | 2 | 5 | 152 | 4 | 157 | 1.08e-66 | 200 |
Find 51 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGCCATGGGATACTTCTT+AGG | 0.198553 | 7:-44195037 | None:intergenic |
| GGCCGATGACGTTGTGCTTT+TGG | 0.259706 | 7:+44195331 | None:intergenic |
| GGTGGTTTGGCGGGTGGTGG+TGG | 0.271046 | 7:+44195449 | None:intergenic |
| TGGATGTGGTACGGCAGATT+GGG | 0.317582 | 7:+44195406 | None:intergenic |
| GGTTTGGCGGGTGGTGGTGG+TGG | 0.318322 | 7:+44195452 | None:intergenic |
| CTTAATATGCATTCTAGATT+GGG | 0.321979 | 7:-44195117 | MsG0780038322.01.T01:CDS |
| CACAACGTCATCGGCCTATC+AGG | 0.331077 | 7:-44195324 | MsG0780038322.01.T01:CDS |
| GAGGGTGGTTTGGCGGGTGG+TGG | 0.335603 | 7:+44195446 | None:intergenic |
| GTGTCAGGTTCTTCTTAATC+AGG | 0.353301 | 7:-44195094 | MsG0780038322.01.T01:CDS |
| GCTTAATATGCATTCTAGAT+TGG | 0.354017 | 7:-44195118 | MsG0780038322.01.T01:CDS |
| GGAGAGGGTGGTTTGGCGGG+TGG | 0.356527 | 7:+44195443 | None:intergenic |
| TTGGGAGAGGTAGGAGAGGG+TGG | 0.371663 | 7:+44195431 | None:intergenic |
| AGAGGTAGGAGAGGGTGGTT+TGG | 0.390987 | 7:+44195436 | None:intergenic |
| TCAGCGCTTTGAGGAGAGTT+TGG | 0.395050 | 7:+44195301 | None:intergenic |
| GTAGGAGAGGGTGGTTTGGC+GGG | 0.395702 | 7:+44195440 | None:intergenic |
| TGAGGAGAGTTTGGCCTGAT+AGG | 0.398932 | 7:+44195310 | None:intergenic |
| TCTTCGAGACGATGCGAATC+GGG | 0.401455 | 7:+44195258 | None:intergenic |
| GTCTTCGAGACGATGCGAAT+CGG | 0.404985 | 7:+44195257 | None:intergenic |
| GGTACGGCAGATTGGGGATT+GGG | 0.411531 | 7:+44195413 | None:intergenic |
| GCATTCTAGATTGGGGTGTC+AGG | 0.413394 | 7:-44195109 | MsG0780038322.01.T01:CDS |
| CTTCTTAATCAGGATCTTCA+AGG | 0.429571 | 7:-44195084 | MsG0780038322.01.T01:CDS |
| GTGGATGTGGTACGGCAGAT+TGG | 0.430512 | 7:+44195405 | None:intergenic |
| GTATCCCATGGCTTAGGTTC+AGG | 0.433041 | 7:+44195045 | None:intergenic |
| GATTGGGGATTGGGAGAGGT+AGG | 0.446647 | 7:+44195422 | None:intergenic |
| AAGCCATGGGATACTTCTTA+GGG | 0.452237 | 7:-44195036 | None:intergenic |
| GGTAGGAGAGGGTGGTTTGG+CGG | 0.459550 | 7:+44195439 | None:intergenic |
| TAATCAGGATCTTCAAGGTA+TGG | 0.466497 | 7:-44195079 | MsG0780038322.01.T01:CDS |
| TGAAGCATAATTCGAGGGCT+AGG | 0.473466 | 7:-44195143 | MsG0780038322.01.T01:CDS |
| CGGTGTTGATCAGCGCTTTG+AGG | 0.497266 | 7:+44195292 | None:intergenic |
| GGCAGATTGGGGATTGGGAG+AGG | 0.499030 | 7:+44195418 | None:intergenic |
| TAAGAAGTATCCCATGGCTT+AGG | 0.505161 | 7:+44195039 | None:intergenic |
| AAATCAACATCGCTCAAGAA+TGG | 0.513106 | 7:-44195209 | MsG0780038322.01.T01:CDS |
| GAAGCATAATTCGAGGGCTA+GGG | 0.513445 | 7:-44195142 | MsG0780038322.01.T01:CDS |
| GGGATTGGGAGAGGTAGGAG+AGG | 0.516094 | 7:+44195427 | None:intergenic |
| TCTTCATCATAACTCCTAGC+TGG | 0.516992 | 7:+44195174 | None:intergenic |
| TGGTACGGCAGATTGGGGAT+TGG | 0.529195 | 7:+44195412 | None:intergenic |
| CTCTTCCTGAACCTAAGCCA+TGG | 0.540626 | 7:-44195050 | MsG0780038322.01.T01:CDS |
| GGCCAAAAGCACAACGTCAT+CGG | 0.541369 | 7:-44195333 | MsG0780038322.01.T01:CDS |
| CGAATCGGGATCGATTAATC+CGG | 0.557877 | 7:+44195272 | None:intergenic |
| CTCAAAGCGCTGATCAACAC+CGG | 0.561818 | 7:-44195291 | MsG0780038322.01.T01:CDS |
| GGATGTGGTACGGCAGATTG+GGG | 0.574360 | 7:+44195407 | None:intergenic |
| TTGGCCTTGGAAGTCGATTG+CGG | 0.587786 | 7:+44195350 | None:intergenic |
| TTAATATGCATTCTAGATTG+GGG | 0.593734 | 7:-44195116 | MsG0780038322.01.T01:CDS |
| GGAAGTCGATTGCGGAGAGA+CGG | 0.599017 | 7:+44195358 | None:intergenic |
| TCTTCCTGAACCTAAGCCAT+GGG | 0.608678 | 7:-44195049 | MsG0780038322.01.T01:CDS |
| GGATTGGGAGAGGTAGGAGA+GGG | 0.612543 | 7:+44195428 | None:intergenic |
| CTCTCCGCAATCGACTTCCA+AGG | 0.623219 | 7:-44195354 | MsG0780038322.01.T01:CDS |
| GGAGAGACGGACGATGCGGT+CGG | 0.642581 | 7:+44195371 | None:intergenic |
| AAACCCTAAGAAGTATCCCA+TGG | 0.667650 | 7:+44195033 | None:intergenic |
| GGCTTGATAAGCTTCCAGCT+AGG | 0.669583 | 7:-44195188 | MsG0780038322.01.T01:CDS |
| TTGCGGAGAGACGGACGATG+CGG | 0.700311 | 7:+44195367 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTTAATATGCATTCTAGATT+GGG | - | Chr7:44195401-44195420 | MsG0780038322.01.T01:CDS | 25.0% |
| ! | TTAATATGCATTCTAGATTG+GGG | - | Chr7:44195402-44195421 | MsG0780038322.01.T01:CDS | 25.0% |
| GCTTAATATGCATTCTAGAT+TGG | - | Chr7:44195400-44195419 | MsG0780038322.01.T01:CDS | 30.0% | |
| !!! | ATGTTTTGAAGCATAATTCG+AGG | - | Chr7:44195369-44195388 | MsG0780038322.01.T01:CDS | 30.0% |
| !!! | TGTTTTGAAGCATAATTCGA+GGG | - | Chr7:44195370-44195389 | MsG0780038322.01.T01:CDS | 30.0% |
| AAATCAACATCGCTCAAGAA+TGG | - | Chr7:44195309-44195328 | MsG0780038322.01.T01:CDS | 35.0% | |
| CTTCTTAATCAGGATCTTCA+AGG | - | Chr7:44195434-44195453 | MsG0780038322.01.T01:CDS | 35.0% | |
| TAATCAGGATCTTCAAGGTA+TGG | - | Chr7:44195439-44195458 | MsG0780038322.01.T01:CDS | 35.0% | |
| GTGTCAGGTTCTTCTTAATC+AGG | - | Chr7:44195424-44195443 | MsG0780038322.01.T01:CDS | 40.0% | |
| TCTTCATCATAACTCCTAGC+TGG | + | Chr7:44195347-44195366 | None:intergenic | 40.0% | |
| !! | TAAGAAGTATCCCATGGCTT+AGG | + | Chr7:44195482-44195501 | None:intergenic | 40.0% |
| CGAATCGGGATCGATTAATC+CGG | + | Chr7:44195249-44195268 | None:intergenic | 45.0% | |
| GAAGCATAATTCGAGGGCTA+GGG | - | Chr7:44195376-44195395 | MsG0780038322.01.T01:CDS | 45.0% | |
| TCTTCCTGAACCTAAGCCAT+GGG | - | Chr7:44195469-44195488 | MsG0780038322.01.T01:CDS | 45.0% | |
| TGAAGCATAATTCGAGGGCT+AGG | - | Chr7:44195375-44195394 | MsG0780038322.01.T01:CDS | 45.0% | |
| !!! | CTTTTTGGGTGGATGTGGTA+CGG | + | Chr7:44195124-44195143 | None:intergenic | 45.0% |
| CTCTTCCTGAACCTAAGCCA+TGG | - | Chr7:44195468-44195487 | MsG0780038322.01.T01:CDS | 50.0% | |
| GGCTTGATAAGCTTCCAGCT+AGG | - | Chr7:44195330-44195349 | MsG0780038322.01.T01:CDS | 50.0% | |
| GTATCCCATGGCTTAGGTTC+AGG | + | Chr7:44195476-44195495 | None:intergenic | 50.0% | |
| GTCTTCGAGACGATGCGAAT+CGG | + | Chr7:44195264-44195283 | None:intergenic | 50.0% | |
| TCTTCGAGACGATGCGAATC+GGG | + | Chr7:44195263-44195282 | None:intergenic | 50.0% | |
| TGAGGAGAGTTTGGCCTGAT+AGG | + | Chr7:44195211-44195230 | None:intergenic | 50.0% | |
| TGGATGTGGTACGGCAGATT+GGG | + | Chr7:44195115-44195134 | None:intergenic | 50.0% | |
| ! | GATGCGGTCGGAGACTTTTT+GGG | + | Chr7:44195138-44195157 | None:intergenic | 50.0% |
| ! | GCATTCTAGATTGGGGTGTC+AGG | - | Chr7:44195409-44195428 | MsG0780038322.01.T01:CDS | 50.0% |
| ! | GGCCAAAAGCACAACGTCAT+CGG | - | Chr7:44195185-44195204 | MsG0780038322.01.T01:CDS | 50.0% |
| ! | TGACGTTGTGCTTTTGGCCT+TGG | + | Chr7:44195184-44195203 | None:intergenic | 50.0% |
| !! | CTCAAAGCGCTGATCAACAC+CGG | - | Chr7:44195227-44195246 | MsG0780038322.01.T01:CDS | 50.0% |
| !! | TCAGCGCTTTGAGGAGAGTT+TGG | + | Chr7:44195220-44195239 | None:intergenic | 50.0% |
| !! | TTGGCCTTGGAAGTCGATTG+CGG | + | Chr7:44195171-44195190 | None:intergenic | 50.0% |
| !!! | GGAGACTTTTTGGGTGGATG+TGG | + | Chr7:44195129-44195148 | None:intergenic | 50.0% |
| !!! | TGGTGGTGGTGGTGAGTTTT+CGG | + | Chr7:44195058-44195077 | None:intergenic | 50.0% |
| AGAGGTAGGAGAGGGTGGTT+TGG | + | Chr7:44195085-44195104 | None:intergenic | 55.0% | |
| CACAACGTCATCGGCCTATC+AGG | - | Chr7:44195194-44195213 | MsG0780038322.01.T01:CDS | 55.0% | |
| GATTGGGGATTGGGAGAGGT+AGG | + | Chr7:44195099-44195118 | None:intergenic | 55.0% | |
| GGAAGTCGATTGCGGAGAGA+CGG | + | Chr7:44195163-44195182 | None:intergenic | 55.0% | |
| GGATGTGGTACGGCAGATTG+GGG | + | Chr7:44195114-44195133 | None:intergenic | 55.0% | |
| GGATTGGGAGAGGTAGGAGA+GGG | + | Chr7:44195093-44195112 | None:intergenic | 55.0% | |
| GGTACGGCAGATTGGGGATT+GGG | + | Chr7:44195108-44195127 | None:intergenic | 55.0% | |
| GTGGATGTGGTACGGCAGAT+TGG | + | Chr7:44195116-44195135 | None:intergenic | 55.0% | |
| TGGTACGGCAGATTGGGGAT+TGG | + | Chr7:44195109-44195128 | None:intergenic | 55.0% | |
| ! | CGATGCGGTCGGAGACTTTT+TGG | + | Chr7:44195139-44195158 | None:intergenic | 55.0% |
| ! | CTCTCCGCAATCGACTTCCA+AGG | - | Chr7:44195164-44195183 | MsG0780038322.01.T01:CDS | 55.0% |
| ! | GGCCGATGACGTTGTGCTTT+TGG | + | Chr7:44195190-44195209 | None:intergenic | 55.0% |
| !! | CGGTGTTGATCAGCGCTTTG+AGG | + | Chr7:44195229-44195248 | None:intergenic | 55.0% |
| GGCAGATTGGGGATTGGGAG+AGG | + | Chr7:44195103-44195122 | None:intergenic | 60.0% | |
| GGGATTGGGAGAGGTAGGAG+AGG | + | Chr7:44195094-44195113 | None:intergenic | 60.0% | |
| TTGCGGAGAGACGGACGATG+CGG | + | Chr7:44195154-44195173 | None:intergenic | 60.0% | |
| TTGGGAGAGGTAGGAGAGGG+TGG | + | Chr7:44195090-44195109 | None:intergenic | 60.0% | |
| ! | GGTAGGAGAGGGTGGTTTGG+CGG | + | Chr7:44195082-44195101 | None:intergenic | 60.0% |
| ! | GTAGGAGAGGGTGGTTTGGC+GGG | + | Chr7:44195081-44195100 | None:intergenic | 60.0% |
| !! | GCGGTCGGAGACTTTTTGGG+TGG | + | Chr7:44195135-44195154 | None:intergenic | 60.0% |
| GGAGAGACGGACGATGCGGT+CGG | + | Chr7:44195150-44195169 | None:intergenic | 65.0% | |
| ! | GAGGGTGGTTTGGCGGGTGG+TGG | + | Chr7:44195075-44195094 | None:intergenic | 70.0% |
| ! | GGAGAGGGTGGTTTGGCGGG+TGG | + | Chr7:44195078-44195097 | None:intergenic | 70.0% |
| ! | GGTGGTTTGGCGGGTGGTGG+TGG | + | Chr7:44195072-44195091 | None:intergenic | 70.0% |
| !! | GGTTTGGCGGGTGGTGGTGG+TGG | + | Chr7:44195069-44195088 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 44195038 | 44195502 | 44195038 | ID=MsG0780038322.01;Name=MsG0780038322.01 |
| Chr7 | mRNA | 44195038 | 44195502 | 44195038 | ID=MsG0780038322.01.T01;Parent=MsG0780038322.01;Name=MsG0780038322.01.T01;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|154 |
| Chr7 | exon | 44195038 | 44195502 | 44195038 | ID=MsG0780038322.01.T01:exon:4807;Parent=MsG0780038322.01.T01 |
| Chr7 | CDS | 44195038 | 44195502 | 44195038 | ID=MsG0780038322.01.T01:cds;Parent=MsG0780038322.01.T01 |
| Gene Sequence |
| Protein sequence |
