Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017665.01.T01 | AES73877.1 | 88.304 | 342 | 13 | 4 | 1 | 339 | 1 | 318 | 0 | 538 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000011.01 | MsG0380017665.01 | 0.839631 | 1.411843e-57 | 1.961264e-54 |
| MsG0180005881.01 | MsG0380017665.01 | 0.821648 | 3.610224e-53 | 2.954482e-50 |
| MsG0380017665.01 | MsG0580024597.01 | -0.813714 | 2.225346e-51 | 1.464234e-48 |
| MsG0380017665.01 | MsG0780038322.01 | 0.816267 | 6.040338e-52 | 4.260242e-49 |
| MsG0380017665.01 | MsG0780041015.01 | 0.825104 | 5.621819e-54 | 5.075830e-51 |
| MsG0380017665.01 | MsG0880042948.01 | -0.820854 | 5.505058e-53 | 4.406662e-50 |
| MsG0380017665.01 | MsG0880043829.01 | -0.814603 | 1.416753e-51 | 9.548688e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017665.01.T01 | MTR_3g109760 | 88.304 | 342 | 13 | 4 | 1 | 339 | 1 | 318 | 0.0 | 538 |
| MsG0380017665.01.T01 | MTR_1g006650 | 51.499 | 367 | 115 | 12 | 6 | 339 | 8 | 344 | 6.14e-105 | 311 |
| MsG0380017665.01.T01 | MTR_8g013950 | 44.959 | 367 | 149 | 14 | 1 | 339 | 1 | 342 | 3.96e-79 | 245 |
| MsG0380017665.01.T01 | MTR_8g076340 | 46.000 | 200 | 85 | 7 | 109 | 298 | 102 | 288 | 1.90e-40 | 144 |
| MsG0380017665.01.T01 | MTR_4g071590 | 34.936 | 312 | 144 | 11 | 1 | 297 | 13 | 280 | 6.71e-40 | 142 |
| MsG0380017665.01.T01 | MTR_4g471590 | 34.936 | 312 | 144 | 11 | 1 | 297 | 13 | 280 | 6.71e-40 | 142 |
| MsG0380017665.01.T01 | MTR_3g117380 | 37.631 | 287 | 142 | 10 | 19 | 298 | 43 | 299 | 5.09e-37 | 135 |
| MsG0380017665.01.T01 | MTR_6g032830 | 37.778 | 225 | 89 | 6 | 120 | 294 | 35 | 258 | 2.03e-35 | 130 |
| MsG0380017665.01.T01 | MTR_1g094930 | 37.872 | 235 | 90 | 7 | 114 | 292 | 72 | 306 | 3.60e-35 | 131 |
| MsG0380017665.01.T01 | MTR_7g085410 | 41.038 | 212 | 82 | 7 | 120 | 292 | 45 | 252 | 4.56e-34 | 126 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017665.01.T01 | AT5G25830 | 46.092 | 371 | 124 | 12 | 2 | 339 | 4 | 331 | 6.02e-84 | 257 |
| MsG0380017665.01.T01 | AT4G32890 | 46.246 | 333 | 116 | 10 | 30 | 339 | 16 | 308 | 7.52e-83 | 254 |
| MsG0380017665.01.T01 | AT3G60530 | 48.454 | 194 | 69 | 4 | 115 | 308 | 72 | 234 | 6.01e-50 | 167 |
| MsG0380017665.01.T01 | AT2G45050 | 48.309 | 207 | 71 | 6 | 115 | 308 | 74 | 257 | 5.28e-48 | 162 |
| MsG0380017665.01.T01 | AT4G36240 | 37.778 | 270 | 113 | 8 | 32 | 295 | 11 | 231 | 1.45e-37 | 135 |
| MsG0380017665.01.T01 | AT5G66320 | 40.254 | 236 | 85 | 5 | 115 | 308 | 108 | 329 | 1.62e-35 | 132 |
| MsG0380017665.01.T01 | AT5G66320 | 40.254 | 236 | 85 | 5 | 115 | 308 | 108 | 329 | 1.62e-35 | 132 |
| MsG0380017665.01.T01 | AT3G24050 | 77.941 | 68 | 15 | 0 | 227 | 294 | 193 | 260 | 7.54e-33 | 123 |
| MsG0380017665.01.T01 | AT1G08010 | 67.073 | 82 | 21 | 1 | 225 | 300 | 217 | 298 | 7.95e-32 | 121 |
| MsG0380017665.01.T01 | AT1G08010 | 67.073 | 82 | 21 | 1 | 225 | 300 | 217 | 298 | 7.95e-32 | 121 |
Find 102 sgRNAs with CRISPR-Local
Find 167 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAGACCTCGGTGATGG+AGG | 0.595010 | 3:-99297872 | MsG0380017665.01.T01:CDS |
| AAATGATTCCTCAGCAAATT+TGG | 0.139979 | 3:+99298153 | None:intergenic |
| AAGACACTATGCAATGCGTG+TGG | 0.676419 | 3:-99297779 | MsG0380017665.01.T01:CDS |
| AAGACTCCACAGTGGCGCAC+CGG | 0.525177 | 3:-99297815 | MsG0380017665.01.T01:CDS |
| AAGGGCCGTCGGCAGAATTC+TGG | 0.309787 | 3:+99299761 | None:intergenic |
| AAGTAATCATCACCGTTGGA+TGG | 0.601160 | 3:+99297569 | None:intergenic |
| AATCAAGTAATCATCACCGT+TGG | 0.595692 | 3:+99297565 | None:intergenic |
| AATGGCCCGGTGCGCCACTG+TGG | 0.562645 | 3:+99297809 | None:intergenic |
| ACAAATGTTGGGCTTGCTGC+AGG | 0.475199 | 3:+99297716 | None:intergenic |
| ACACCCTCAAGTGTCTGTCC+CGG | 0.593472 | 3:-99298035 | MsG0380017665.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATGATTCCTCAGCAAATT+TGG | + | Chr3:99299379-99299398 | None:intergenic | 30.0% | |
| ATATATATAATGGCACGTAC+CGG | + | Chr3:99297814-99297833 | None:intergenic | 30.0% | |
| ATCTGTTCCATTGAATTTGA+GGG | - | Chr3:99297950-99297969 | MsG0380017665.01.T01:CDS | 30.0% | |
| TATCTGTTCCATTGAATTTG+AGG | - | Chr3:99297949-99297968 | MsG0380017665.01.T01:CDS | 30.0% | |
| AATCAAGTAATCATCACCGT+TGG | + | Chr3:99299967-99299986 | None:intergenic | 35.0% | |
| AGCAGTGTTTACATTGTTTG+TGG | + | Chr3:99297587-99297606 | None:intergenic | 35.0% | |
| AGTGTTTACATTGTTTGTGG+TGG | + | Chr3:99297584-99297603 | None:intergenic | 35.0% | |
| ATTGACGTCGGTGAAAAAAT+TGG | + | Chr3:99297743-99297762 | None:intergenic | 35.0% | |
| ATTGGTTAAGCAGCTAAAAG+AGG | - | Chr3:99298243-99298262 | MsG0380017665.01.T01:intron | 35.0% | |
| CATCACAGCATTATGTTTGA+AGG | - | Chr3:99299936-99299955 | MsG0380017665.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 99297526 | 99300025 | 99297526 | ID=MsG0380017665.01;Name=MsG0380017665.01 |
| Chr3 | mRNA | 99297526 | 99300025 | 99297526 | ID=MsG0380017665.01.T01;Parent=MsG0380017665.01;Name=MsG0380017665.01.T01;_AED=0.33;_eAED=0.36;_QI=0|0|0|1|1|1|3|0|339 |
| Chr3 | exon | 99299738 | 99300025 | 99299738 | ID=MsG0380017665.01.T01:exon:35672;Parent=MsG0380017665.01.T01 |
| Chr3 | exon | 99299287 | 99299334 | 99299287 | ID=MsG0380017665.01.T01:exon:35671;Parent=MsG0380017665.01.T01 |
| Chr3 | exon | 99297526 | 99298209 | 99297526 | ID=MsG0380017665.01.T01:exon:35670;Parent=MsG0380017665.01.T01 |
| Chr3 | CDS | 99299738 | 99300025 | 99299738 | ID=MsG0380017665.01.T01:cds;Parent=MsG0380017665.01.T01 |
| Chr3 | CDS | 99299287 | 99299334 | 99299287 | ID=MsG0380017665.01.T01:cds;Parent=MsG0380017665.01.T01 |
| Chr3 | CDS | 99297526 | 99298209 | 99297526 | ID=MsG0380017665.01.T01:cds;Parent=MsG0380017665.01.T01 |
| Gene Sequence |
>MsG0380017665.01.T01 ATGGAAGCTCAAGAGTTTTTCCAAACCAGCTTCTGTCCACAATCCGACAACAATTCCACCACAAACAATGTAAACACTGCTGCTTCCGACCACTTCATCGTTGAAGATCTCTTTGATTTCTCTAATGAGGATGTTGCCATTGAAGACCCCACTTTCGAAGAATCTCCTCCTACCAACTCTAATGATTCTCCTCCTCTTGAAATCAATCCCACTTCCAATTTTTTCACCGACGTCAATTCGTGCCAGAATTCTGCCGACGGCCCTTTCTCCGGCGAACTCTCCGTTCCGGAGAATGTTAACCGGTGCCCCGGGGCATTGGTTAAGCAGCTAAAAGAGCAATATGATGATCTTGCGCAGCTAGAATGGATGTCCAAATTTGCTGAGGAATCATTTTCAAGTGAGGACCTTCACAAGCTACAGTTGATATCTGGATTAAAGGCACCAAATAACGTTGAATCAAAAACTCATGAAGAACGCAACCCAACAATACACCCTCAAGTGTCTGTCCCGGCAAAAGCCCGTAGTAAACGGTCACGTGTGCCTCCATGCAACTGGACTTCGCGCCTTCTAGTTCTCTCTCCAACCACGACCACCACCACCACATCTTCTCACTCTGACACCATGGCTCCACCAAAAAAGCCCTCTCCAAGAAAAAGAGACCTCGGTGATGGAGGAGAGGGTCGCAAGTGTCTTCATTGCGCCACTGATAAGACTCCACAGTGGCGCACCGGGCCATTAGGCCCAAAGACACTATGCAATGCGTGTGGAGTCAGATACAAGTCAGGTCGACTCGTACCTGAGTATAGGCCTGCAGCAAGCCCAACATTTGTGTTGACTAAGCACTCCAACTCACATCGAAAGGTGCAAGAGCTCCGACGCCAGAAGGAGATGATGAGGGCCCAACAACACCAATTGTTGCAACTACAACATCATCACAGCATTATGTTTGAAGGACCATCCAACGGTGATGATTACTTGATTCATCAACACGTGGGACCCGATTTCACGCACCTCATGTAG |
| Protein sequence |
>MsG0380017665.01.T01 MEAQEFFQTSFCPQSDNNSTTNNVNTAASDHFIVEDLFDFSNEDVAIEDPTFEESPPTNSNDSPPLEINPTSNFFTDVNSCQNSADGPFSGELSVPENVNRCPGALVKQLKEQYDDLAQLEWMSKFAEESFSSEDLHKLQLISGLKAPNNVESKTHEERNPTIHPQVSVPAKARSKRSRVPPCNWTSRLLVLSPTTTTTTTSSHSDTMAPPKKPSPRKRDLGDGGEGRKCLHCATDKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVLTKHSNSHRKVQELRRQKEMMRAQQHQLLQLQHHHSIMFEGPSNGDDYLIHQHVGPDFTHLM* |
