Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039356.01.T01 | RHN69186.1 | 93.164 | 512 | 33 | 1 | 96 | 605 | 39 | 550 | 0 | 984 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039356.01.T01 | A0A396ITZ6 | 93.164 | 512 | 33 | 1 | 96 | 605 | 39 | 550 | 0.0 | 984 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380015136.01 | MsG0780039356.01 | 0.864210 | 1.432219e-64 | 4.460672e-61 |
| MsG0380016154.01 | MsG0780039356.01 | 0.916210 | 2.343405e-85 | 6.375250e-81 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039356.01.T01 | MTR_3g084520 | 93.920 | 477 | 29 | 0 | 129 | 605 | 1 | 477 | 0.0 | 932 |
| MsG0780039356.01.T01 | MTR_3g084530 | 80.720 | 472 | 91 | 0 | 129 | 600 | 1 | 472 | 0.0 | 792 |
| MsG0780039356.01.T01 | MTR_3g084540 | 79.332 | 479 | 98 | 1 | 129 | 606 | 1 | 479 | 0.0 | 790 |
| MsG0780039356.01.T01 | MTR_8g083290 | 58.824 | 476 | 175 | 8 | 133 | 601 | 3 | 464 | 0.0 | 541 |
| MsG0780039356.01.T01 | MTR_8g083280 | 58.144 | 485 | 166 | 11 | 129 | 600 | 1 | 461 | 0.0 | 534 |
| MsG0780039356.01.T01 | MTR_6g038300 | 41.365 | 469 | 251 | 9 | 134 | 601 | 11 | 456 | 2.43e-111 | 341 |
| MsG0780039356.01.T01 | MTR_5g035580 | 40.213 | 470 | 249 | 12 | 132 | 598 | 11 | 451 | 1.80e-104 | 324 |
| MsG0780039356.01.T01 | MTR_6g038240 | 40.803 | 473 | 251 | 10 | 131 | 600 | 8 | 454 | 1.06e-103 | 322 |
| MsG0780039356.01.T01 | MTR_6g038200 | 39.915 | 471 | 254 | 11 | 134 | 601 | 11 | 455 | 4.91e-100 | 312 |
| MsG0780039356.01.T01 | MTR_6g038230 | 39.381 | 485 | 250 | 16 | 131 | 605 | 6 | 456 | 4.53e-98 | 307 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039356.01.T01 | AT4G15550 | 49.380 | 484 | 203 | 13 | 134 | 599 | 13 | 472 | 1.70e-152 | 447 |
| MsG0780039356.01.T01 | AT4G14090 | 46.596 | 470 | 219 | 10 | 134 | 599 | 13 | 454 | 1.65e-138 | 411 |
| MsG0780039356.01.T01 | AT1G05560 | 48.340 | 482 | 213 | 13 | 134 | 606 | 5 | 459 | 1.67e-138 | 412 |
| MsG0780039356.01.T01 | AT1G05560 | 47.782 | 496 | 221 | 14 | 122 | 606 | 41 | 509 | 2.33e-138 | 413 |
| MsG0780039356.01.T01 | AT1G05530 | 46.555 | 479 | 224 | 11 | 129 | 600 | 1 | 454 | 4.15e-136 | 405 |
| MsG0780039356.01.T01 | AT2G43820 | 40.000 | 475 | 246 | 15 | 134 | 602 | 7 | 448 | 4.26e-100 | 312 |
| MsG0780039356.01.T01 | AT1G24100 | 37.577 | 487 | 265 | 14 | 125 | 601 | 2 | 459 | 3.26e-94 | 297 |
| MsG0780039356.01.T01 | AT1G05675 | 37.815 | 476 | 264 | 11 | 129 | 598 | 1 | 450 | 2.83e-93 | 295 |
| MsG0780039356.01.T01 | AT2G43840 | 37.288 | 472 | 259 | 12 | 134 | 600 | 7 | 446 | 3.94e-93 | 294 |
| MsG0780039356.01.T01 | AT2G43840 | 37.632 | 473 | 256 | 13 | 134 | 600 | 7 | 446 | 1.89e-92 | 292 |
Find 151 sgRNAs with CRISPR-Local
Find 231 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGGTGTTGTCAGGTGG+AGG | 0.490927 | 7:+62660859 | MsG0780039356.01.T01:CDS |
| AAAGTGTTTGGAAGTGGTGA+TGG | 0.499781 | 7:+62661087 | MsG0780039356.01.T01:CDS |
| AAATAAGACGTTTGGCGAAT+TGG | 0.302349 | 7:-62659881 | None:intergenic |
| AACTTGAGAATAATATGAAT+GGG | 0.497468 | 7:+62660828 | MsG0780039356.01.T01:CDS |
| AAGAAAGCAGATGGAATCAA+CGG | 0.616218 | 7:-62660530 | None:intergenic |
| AAGAAGGAGGGTCTTCTGGT+AGG | 0.612081 | 7:+62661182 | MsG0780039356.01.T01:CDS |
| AAGATCAAAATGATACCCAT+CGG | 0.634310 | 7:+62660506 | MsG0780039356.01.T01:CDS |
| AAGGAGAGGCCGGGAATGGT+TGG | 0.502271 | 7:-62659966 | None:intergenic |
| AAGGTGGCGAAGGAGAGGCC+GGG | 0.606277 | 7:-62659975 | None:intergenic |
| AAGTGTTTATATAATGGTGG+TGG | 0.619257 | 7:-62659702 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATCAACCAATGTAGGA+CGG | + | Chr7:62658722-62658741 | MsG0780039356.01.T01:intron | 30.0% | |
| AAGATCAAAATGATACCCAT+CGG | + | Chr7:62660506-62660525 | MsG0780039356.01.T01:CDS | 30.0% | |
| AAGTGTTTATATAATGGTGG+TGG | - | Chr7:62659705-62659724 | None:intergenic | 30.0% | |
| ACTAAATAAAGAACGACAAC+AGG | - | Chr7:62658526-62658545 | None:intergenic | 30.0% | |
| ATAAGAGTTTCGATACAATC+GGG | - | Chr7:62658663-62658682 | None:intergenic | 30.0% | |
| CAAAGGATGATTACATTGAA+TGG | + | Chr7:62660609-62660628 | MsG0780039356.01.T01:CDS | 30.0% | |
| CAAAGTTAATTGAAGATGTG+TGG | + | Chr7:62661005-62661024 | MsG0780039356.01.T01:CDS | 30.0% | |
| CTACATCACAAACAAATCAA+AGG | + | Chr7:62660271-62660290 | MsG0780039356.01.T01:CDS | 30.0% | |
| GATACTTATGATGCCATTAT+TGG | - | Chr7:62659654-62659673 | None:intergenic | 30.0% | |
| GGAAATAATACAGCCAAATT+CGG | - | Chr7:62658505-62658524 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 62658252 | 62661252 | 62658252 | ID=MsG0780039356.01;Name=MsG0780039356.01 |
| Chr7 | mRNA | 62658252 | 62661252 | 62658252 | ID=MsG0780039356.01.T01;Parent=MsG0780039356.01;Name=MsG0780039356.01.T01;_AED=0.15;_eAED=0.18;_QI=0|0|0|1|0|0|2|0|606 |
| Chr7 | exon | 62658252 | 62658499 | 62658252 | ID=MsG0780039356.01.T01:exon:16145;Parent=MsG0780039356.01.T01 |
| Chr7 | exon | 62659680 | 62661252 | 62659680 | ID=MsG0780039356.01.T01:exon:16144;Parent=MsG0780039356.01.T01 |
| Chr7 | CDS | 62658252 | 62658499 | 62658252 | ID=MsG0780039356.01.T01:cds;Parent=MsG0780039356.01.T01 |
| Chr7 | CDS | 62659680 | 62661252 | 62659680 | ID=MsG0780039356.01.T01:cds;Parent=MsG0780039356.01.T01 |
| Gene Sequence |
>MsG0780039356.01.T01 GAGGCTGATGCTGCTTTCTCTTCTCACCTCAACCGCACATGCATTGACATCTGTATTCGTGATTCTGTTGGCATATTTGTGTTGGCTAAGGCTGTGAGTTATCCGTGCTGCGTTTCTATTGACGTGGGTGAAGCTTTAGGTTTGCACTCGACGTTACAGTGGTTTGATGATATGCATTTTGATAGTGTCGACTTCGAAACGGATTCTAAACTGACGGCTGATGCTTTTCTGTCTAATAGGAATGACACAGATTCGTCACCGCAAACGTCACCACCACCATTATATAAACACTTCTCCTATCCATCCATACATGCATCTCTTGTCACACATTTCCTTCGCACTCCATTCCCTAGTACCATTGCATTTTCACAAGCCACCACTACCATGGCGCAAAACCACCACTTCCTCATTATTACATACCCAATACAAGGTCACATAAACCCTGCTCTCCAATTCGCCAAACGTCTTATTTCTTTGGGAGCAAAAGTCACTTTTGCTACCACTATCCACTTATACAGCCGCCTTATCAACAAACCAACCATTCCCGGCCTCTCCTTCGCCACCTTCTCTGACGGCTACGACAACGGTCAAAAATCCTTTGGCGACGAAGACATCGTCTCCTACATGTCCGAGTTTACGCGCCACGGCTCAGAATTTCTCACAAATATCATTCTCTCCTCCAAACAAGAAAATCATCCATTCACTTGCTTAATCTACACTCTCATACTTTCATGGGCTCCCAAGGTGGCGCATGAGCTTCACCTCCCATCAGCAATGCTGTGGATTCAAGCAGCAACGGTGTTTGATATATTCTATTATTACTTTCATGAACACGGTGACTACATCACAAACAAATCAAAGGATGAAACATGTTCAATATCATTGCCAGGATTGTCATTCTCTCTTAAAAGCTGGGACCTTCCCTCTTTTTTATTGGCTTCAAATACATACACTTTTGCTCTTCCATCTCTCAAAGAGCAAATTCAGTTGTTGAATGAAGAAAACAACCCAAGAGTACTTGTCAACACCGTTGAGGAGTTTGAACTCGATGCACTGAGAGAAGTTGATGTTGGTAAGATCAAAATGATACCCATCGGACCGTTGATTCCATCTGCTTTCTTGGATGGCAAGGATCCTACGGATAATTCGTTTGGCGGTGACATTGTCCGTGTTGACTCAAAGGATGATTACATTGAATGGTTGGATTCGAAGGATGAATCGTCTGTGGTTTATGTTTCATTTGGTACTCTTGCTGTTTTGTCTAAGAGACAAATGGAGGAAATTGCACGTGCCTTGTTGGATTCTGGATTTTCTTTCTTGTGGGTAATTAGAGATAAAAAAAATCAACAACAAAAAGAAGTTGATGATGATGATGATGAGCTGAGTTGTAGAGAGGAACTTGAGAATAATATGAATGGGAAAATAGTAAAATGGTGTTGTCAGGTGGAGGTTTTATCGCACCGTTCTTTGGGTTGTTTTGTGACACATTGTGGATGGAATTCTACTTTGGAAAGTCTAGGTTCAGGGGTTCCTATGGTTGCATTTCCTCAGTGGACAGATCAAACTACTAATGCAAAGTTAATTGAAGATGTGTGGAAGACTGGGGTGAGGATGGAACGCGATGAGGAAGGGGTGGTGAAAGCTGAGGAAATTAGAAAGTGTTTGGAAGTGGTGATGGGGAAGGGAGAAAAAGGAGAGGAACTAAGGAGCAATGCTATGAAATGGAAAGATTTGGCTAGGGCAGCTGTGAAAGAAGGAGGGTCTTCTGGTAGGAATTTGAGGAGTTTTCTAAATGATATTGCAAGTATAACTCAAAATTAG |
| Protein sequence |
>MsG0780039356.01.T01 EADAAFSSHLNRTCIDICIRDSVGIFVLAKAVSYPCCVSIDVGEALGLHSTLQWFDDMHFDSVDFETDSKLTADAFLSNRNDTDSSPQTSPPPLYKHFSYPSIHASLVTHFLRTPFPSTIAFSQATTTMAQNHHFLIITYPIQGHINPALQFAKRLISLGAKVTFATTIHLYSRLINKPTIPGLSFATFSDGYDNGQKSFGDEDIVSYMSEFTRHGSEFLTNIILSSKQENHPFTCLIYTLILSWAPKVAHELHLPSAMLWIQAATVFDIFYYYFHEHGDYITNKSKDETCSISLPGLSFSLKSWDLPSFLLASNTYTFALPSLKEQIQLLNEENNPRVLVNTVEEFELDALREVDVGKIKMIPIGPLIPSAFLDGKDPTDNSFGGDIVRVDSKDDYIEWLDSKDESSVVYVSFGTLAVLSKRQMEEIARALLDSGFSFLWVIRDKKNQQQKEVDDDDDELSCREELENNMNGKIVKWCCQVEVLSHRSLGCFVTHCGWNSTLESLGSGVPMVAFPQWTDQTTNAKLIEDVWKTGVRMERDEEGVVKAEEIRKCLEVVMGKGEKGEELRSNAMKWKDLARAAVKEGGSSGRNLRSFLNDIASITQN* |
