AlfalfaGEDB | MsG0780039362.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039362.01

KEGG: -
GO: -
PFAM: PF07107

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039362.01.T01	XP_003623505.1	96.622	148	5	0	1	148	4	151	9.93E-104	303

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039362.01.T01	Q94AK6	39.091	110	58	3	48	148	1	110	3.60E-16	72.4

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039362.01.T01	G7KVY7	96.622	148	5	0	1	148	4	151	4.74e-104	303

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005039.01	MsG0780039362.01	0.800349	1.502670e-48	6.981213e-46
MsG0480020783.01	MsG0780039362.01	0.811894	5.572119e-51	3.490882e-48
MsG0480023565.01	MsG0780039362.01	0.800972	1.121430e-48	5.293225e-46
MsG0380015693.01	MsG0780039362.01	0.810056	1.393710e-50	8.317354e-48
MsG0380016192.01	MsG0780039362.01	0.826149	3.179269e-54	2.957118e-51
MsG0780039362.01	MsG0780039364.01	0.868016	8.928323e-66	3.181011e-62
MsG0780039362.01	MsG0780039365.01	0.815302	9.911782e-52	6.808024e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039362.01.T01	MTR_7g071760	96.622	148	5	0	1	148	4	151	1.20e-107	303
MsG0780039362.01.T01	MTR_7g071790	95.946	148	6	0	1	148	4	151	4.90e-107	301
MsG0780039362.01.T01	MTR_7g071840	95.946	148	6	0	1	148	4	151	6.38e-107	301
MsG0780039362.01.T01	MTR_7g071970	95.270	148	7	0	1	148	4	151	6.59e-107	301
MsG0780039362.01.T01	MTR_7g071820	93.919	148	7	1	1	148	4	149	4.73e-102	289
MsG0780039362.01.T01	MTR_3g031270	49.664	149	64	5	2	148	23	162	1.34e-40	134
MsG0780039362.01.T01	MTR_3g072030	38.816	152	86	3	3	148	50	200	5.96e-29	105
MsG0780039362.01.T01	MTR_5g074500	37.908	153	83	7	3	148	6	153	1.43e-22	87.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039362.01.T01	AT5G01740	53.947	152	60	5	3	148	15	162	6.16e-50	157
MsG0780039362.01.T01	AT3G10985	39.091	110	58	3	48	148	1	110	3.67e-17	72.4

Find 35 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATGATCTTCCCACTTTAA+TGG	0.211798	7:-62765216	None:intergenic
AATTCTCTACAAAGCACTTT+TGG	0.217894	7:+62764906	MsG0780039362.01.T01:CDS
CCAATTGAAGTGACACTTCT+TGG	0.219369	7:-62765066	None:intergenic
GGTGGGAAGGACAAGCTTAT+TGG	0.263235	7:+62765109	MsG0780039362.01.T01:CDS
AAGATCACTTACTAGCAATT+TGG	0.285290	7:-62764953	None:intergenic
AAGCTTATTGGGTTCATGTT+TGG	0.330474	7:+62765121	MsG0780039362.01.T01:CDS
GTGGGAAGGACAAGCTTATT+GGG	0.379366	7:+62765110	MsG0780039362.01.T01:CDS
CACATGATGAAGGTGTTAAC+AGG	0.392054	7:+62765009	MsG0780039362.01.T01:CDS
CCAAGAAGTGTCACTTCAAT+TGG	0.410079	7:+62765066	MsG0780039362.01.T01:CDS
GGAGAAACAAATCACACAAA+AGG	0.412936	7:+62765030	MsG0780039362.01.T01:CDS
CTTTATCGTAGGTCACTTCC+TGG	0.413885	7:+62765279	MsG0780039362.01.T01:CDS
GTCACTTCCTGGACTTGTCT+TGG	0.440729	7:+62765290	MsG0780039362.01.T01:CDS
GGGTCAAGGAACAATGGAAA+TGG	0.460181	7:+62764927	MsG0780039362.01.T01:CDS
GATCTTGAGTATTGGTTCCA+TGG	0.486528	7:+62764970	MsG0780039362.01.T01:CDS
GAGAAACAAATCACACAAAA+GGG	0.502625	7:+62765031	MsG0780039362.01.T01:CDS
TTTGTAGAGAATTTCAACTA+TGG	0.506167	7:-62764896	None:intergenic
TTAGAGAGTATTTCAATACA+TGG	0.507160	7:+62765172	MsG0780039362.01.T01:CDS
TAAATAGCCAAGACAAGTCC+AGG	0.520105	7:-62765297	None:intergenic
GAGATTTGAGGCCATTAAAG+TGG	0.528323	7:+62765205	MsG0780039362.01.T01:CDS
TAGTAAGTGATCTTGAGTAT+TGG	0.552212	7:+62764962	MsG0780039362.01.T01:CDS
GGCTTGTAGTGAGAGATTTG+AGG	0.557987	7:+62765193	MsG0780039362.01.T01:CDS
ATTGTGTAATAGCTGAGGGG+TGG	0.579146	7:+62765091	MsG0780039362.01.T01:CDS
AGATTTGAGGCCATTAAAGT+GGG	0.590336	7:+62765206	MsG0780039362.01.T01:CDS
TTGTGTAATAGCTGAGGGGT+GGG	0.596289	7:+62765092	MsG0780039362.01.T01:CDS
ACAATGTCATCACATGATGA+AGG	0.602094	7:+62764999	MsG0780039362.01.T01:CDS
GTAATAGCTGAGGGGTGGGA+AGG	0.612724	7:+62765096	MsG0780039362.01.T01:CDS
GATGACATTGTGGTGGACCA+TGG	0.629400	7:-62764987	None:intergenic
TTCATCATGTGATGACATTG+TGG	0.642595	7:-62764997	None:intergenic
AGCAAGATAACATGACATTG+TGG	0.661544	7:+62765238	MsG0780039362.01.T01:CDS
ATCATGTGATGACATTGTGG+TGG	0.671314	7:-62764994	None:intergenic
GGTGATTGTGTAATAGCTGA+GGG	0.672963	7:+62765087	MsG0780039362.01.T01:CDS
TGGTGATTGTGTAATAGCTG+AGG	0.675657	7:+62765086	MsG0780039362.01.T01:CDS
AACCTCGTGATCTTTATCGT+AGG	0.678620	7:+62765268	MsG0780039362.01.T01:CDS
GACCTACGATAAAGATCACG+AGG	0.717672	7:-62765270	None:intergenic
GTGATTGTGTAATAGCTGAG+GGG	0.773023	7:+62765088	MsG0780039362.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGTTTGGACATTGAAAAAT+GGG	+	Chr7:62765136-62765155	MsG0780039362.01.T01:CDS	25.0%
!	TTTGTAGAGAATTTCAACTA+TGG	-	Chr7:62764899-62764918	None:intergenic	25.0%
!!!	TTAGAGAGTATTTCAATACA+TGG	+	Chr7:62765172-62765191	MsG0780039362.01.T01:CDS	25.0%
	AAGATCACTTACTAGCAATT+TGG	-	Chr7:62764956-62764975	None:intergenic	30.0%
	CATGTTTGGACATTGAAAAA+TGG	+	Chr7:62765135-62765154	MsG0780039362.01.T01:CDS	30.0%
	GAGAAACAAATCACACAAAA+GGG	+	Chr7:62765031-62765050	MsG0780039362.01.T01:CDS	30.0%
	TAGTAAGTGATCTTGAGTAT+TGG	+	Chr7:62764962-62764981	MsG0780039362.01.T01:CDS	30.0%
	TTATGATCTTCCCACTTTAA+TGG	-	Chr7:62765219-62765238	None:intergenic	30.0%
!!	AATTCTCTACAAAGCACTTT+TGG	+	Chr7:62764906-62764925	MsG0780039362.01.T01:CDS	30.0%
!!	ATTCTCTACAAAGCACTTTT+GGG	+	Chr7:62764907-62764926	MsG0780039362.01.T01:CDS	30.0%
	AAGCTTATTGGGTTCATGTT+TGG	+	Chr7:62765121-62765140	MsG0780039362.01.T01:CDS	35.0%
	ACAATGTCATCACATGATGA+AGG	+	Chr7:62764999-62765018	MsG0780039362.01.T01:CDS	35.0%
	AGCAAGATAACATGACATTG+TGG	+	Chr7:62765238-62765257	MsG0780039362.01.T01:CDS	35.0%
	GGAGAAACAAATCACACAAA+AGG	+	Chr7:62765030-62765049	MsG0780039362.01.T01:CDS	35.0%
	TTCATCATGTGATGACATTG+TGG	-	Chr7:62765000-62765019	None:intergenic	35.0%
!	AGATTTGAGGCCATTAAAGT+GGG	+	Chr7:62765206-62765225	MsG0780039362.01.T01:CDS	35.0%
	AACCTCGTGATCTTTATCGT+AGG	+	Chr7:62765268-62765287	MsG0780039362.01.T01:CDS	40.0%
	ATCATGTGATGACATTGTGG+TGG	-	Chr7:62764997-62765016	None:intergenic	40.0%
	CCAATTGAAGTGACACTTCT+TGG	-	Chr7:62765069-62765088	None:intergenic	40.0%
	GGTGATTGTGTAATAGCTGA+GGG	+	Chr7:62765087-62765106	MsG0780039362.01.T01:CDS	40.0%
	GTGATTGTGTAATAGCTGAG+GGG	+	Chr7:62765088-62765107	MsG0780039362.01.T01:CDS	40.0%
	TAAATAGCCAAGACAAGTCC+AGG	-	Chr7:62765300-62765319	None:intergenic	40.0%
	TGGTGATTGTGTAATAGCTG+AGG	+	Chr7:62765086-62765105	MsG0780039362.01.T01:CDS	40.0%
!	GAGATTTGAGGCCATTAAAG+TGG	+	Chr7:62765205-62765224	MsG0780039362.01.T01:CDS	40.0%
!!	ACTTTTGGGTCAAGGAACAA+TGG	+	Chr7:62764921-62764940	MsG0780039362.01.T01:CDS	40.0%
!!	CACATGATGAAGGTGTTAAC+AGG	+	Chr7:62765009-62765028	MsG0780039362.01.T01:CDS	40.0%
!!	CCAAGAAGTGTCACTTCAAT+TGG	+	Chr7:62765066-62765085	MsG0780039362.01.T01:CDS	40.0%
!!	GATCTTGAGTATTGGTTCCA+TGG	+	Chr7:62764970-62764989	MsG0780039362.01.T01:CDS	40.0%
!!	TACAAAGCACTTTTGGGTCA+AGG	+	Chr7:62764913-62764932	MsG0780039362.01.T01:CDS	40.0%
	ATTGTGTAATAGCTGAGGGG+TGG	+	Chr7:62765091-62765110	MsG0780039362.01.T01:CDS	45.0%
	CTTTATCGTAGGTCACTTCC+TGG	+	Chr7:62765279-62765298	MsG0780039362.01.T01:CDS	45.0%
	GACCTACGATAAAGATCACG+AGG	-	Chr7:62765273-62765292	None:intergenic	45.0%
	GGCTTGTAGTGAGAGATTTG+AGG	+	Chr7:62765193-62765212	MsG0780039362.01.T01:CDS	45.0%
	GGGTCAAGGAACAATGGAAA+TGG	+	Chr7:62764927-62764946	MsG0780039362.01.T01:CDS	45.0%
	GTGGGAAGGACAAGCTTATT+GGG	+	Chr7:62765110-62765129	MsG0780039362.01.T01:CDS	45.0%
	TTGTGTAATAGCTGAGGGGT+GGG	+	Chr7:62765092-62765111	MsG0780039362.01.T01:CDS	45.0%
	GGTGGGAAGGACAAGCTTAT+TGG	+	Chr7:62765109-62765128	MsG0780039362.01.T01:CDS	50.0%
	GTCACTTCCTGGACTTGTCT+TGG	+	Chr7:62765290-62765309	MsG0780039362.01.T01:CDS	50.0%
!!	GATGACATTGTGGTGGACCA+TGG	-	Chr7:62764990-62765009	None:intergenic	50.0%
	GTAATAGCTGAGGGGTGGGA+AGG	+	Chr7:62765096-62765115	MsG0780039362.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	62764874	62765320	62764874	ID=MsG0780039362.01;Name=MsG0780039362.01
Chr7	mRNA	62764874	62765320	62764874	ID=MsG0780039362.01.T01;Parent=MsG0780039362.01;Name=MsG0780039362.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr7	exon	62764874	62765320	62764874	ID=MsG0780039362.01.T01:exon:16946;Parent=MsG0780039362.01.T01
Chr7	CDS	62764874	62765320	62764874	ID=MsG0780039362.01.T01:cds;Parent=MsG0780039362.01.T01

Gene Sequence

>MsG0780039362.01.T01

ATGGTTGAAATGCAAAACAAGTCCATAGTTGAAATTCTCTACAAAGCACTTTTGGGTCAAGGAACAATGGAAATGGTAGCCAAATTGCTAGTAAGTGATCTTGAGTATTGGTTCCATGGTCCACCACAATGTCATCACATGATGAAGGTGTTAACAGGAGAAACAAATCACACAAAAGGGTTCAAATTTGAGCCAAGAAGTGTCACTTCAATTGGTGATTGTGTAATAGCTGAGGGGTGGGAAGGACAAGCTTATTGGGTTCATGTTTGGACATTGAAAAATGGGTTAATTACACAATTTAGAGAGTATTTCAATACATGGCTTGTAGTGAGAGATTTGAGGCCATTAAAGTGGGAAGATCATAAGCAAGATAACATGACATTGTGGAGAAGTCAACCTCGTGATCTTTATCGTAGGTCACTTCCTGGACTTGTCTTGGCTATTTAG

Protein sequence

>MsG0780039362.01.T01

MVEMQNKSIVEILYKALLGQGTMEMVAKLLVSDLEYWFHGPPQCHHMMKVLTGETNHTKGFKFEPRSVTSIGDCVIAEGWEGQAYWVHVWTLKNGLITQFREYFNTWLVVRDLRPLKWEDHKQDNMTLWRSQPRDLYRRSLPGLVLAI*