AlfalfaGEDB | MsG0780039364.01

M. sativa cultivar ZhongmuNo.1 / MsG0780039364.01

KEGG: -
GO: -
PFAM: PF07107

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039364.01.T01	XP_003623505.1	98.649	148	2	0	1	148	4	151	2.65E-106	309

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039364.01.T01	Q94AK6	38.182	110	59	3	48	148	1	110	2.08E-15	70.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039364.01.T01	G7KVY7	98.649	148	2	0	1	148	4	151	1.26e-106	309

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049068.01	MsG0780039364.01	0.807470	4.975628e-50	2.773782e-47
MsG0180000292.01	MsG0780039364.01	0.807829	4.174076e-50	2.348653e-47
MsG0180001085.01	MsG0780039364.01	0.804744	1.864445e-49	9.690308e-47
MsG0180004542.01	MsG0780039364.01	0.820288	7.425141e-53	5.850646e-50
MsG0180005039.01	MsG0780039364.01	0.813200	2.886608e-51	1.872817e-48
MsG0180005807.01	MsG0780039364.01	0.806390	8.419272e-50	4.564087e-47
MsG0280010975.01	MsG0780039364.01	0.825879	3.683855e-54	3.400429e-51
MsG0380011495.01	MsG0780039364.01	0.805116	1.559446e-49	8.182301e-47
MsG0680031715.01	MsG0780039364.01	0.818087	2.354098e-52	1.745051e-49
MsG0680032559.01	MsG0780039364.01	0.841733	3.972258e-58	5.890072e-55
MsG0680033377.01	MsG0780039364.01	0.808981	2.371071e-50	1.375197e-47
MsG0680034767.01	MsG0780039364.01	0.805762	1.141479e-49	6.089885e-47
MsG0480019711.01	MsG0780039364.01	0.801350	9.385505e-49	4.472317e-46
MsG0480020690.01	MsG0780039364.01	0.814140	1.793136e-51	1.193498e-48
MsG0480020783.01	MsG0780039364.01	0.835293	1.825301e-56	2.222745e-53
MsG0480021060.01	MsG0780039364.01	0.802569	5.272832e-49	2.591423e-46
MsG0480022260.01	MsG0780039364.01	0.820865	5.472237e-53	4.381865e-50
MsG0480022514.01	MsG0780039364.01	0.838263	3.190884e-57	4.250658e-54
MsG0480022624.01	MsG0780039364.01	0.821134	4.745558e-53	3.828394e-50
MsG0480023381.01	MsG0780039364.01	0.806336	8.645328e-50	4.680146e-47
MsG0480023565.01	MsG0780039364.01	0.802372	5.789351e-49	2.831272e-46
MsG0480023578.01	MsG0780039364.01	0.805157	1.528961e-49	8.030799e-47
MsG0580025411.01	MsG0780039364.01	0.825300	5.054076e-54	4.588713e-51
MsG0580025668.01	MsG0780039364.01	0.800242	1.580215e-48	7.321231e-46
MsG0380015603.01	MsG0780039364.01	0.816332	5.839931e-52	4.126365e-49
MsG0380015693.01	MsG0780039364.01	0.839125	1.910433e-57	2.612836e-54
MsG0380015896.01	MsG0780039364.01	0.819165	1.340386e-52	1.023627e-49
MsG0380016192.01	MsG0780039364.01	0.847807	9.155846e-60	1.646583e-56
MsG0380017336.01	MsG0780039364.01	0.814815	1.271149e-51	8.616443e-49
MsG0380018069.01	MsG0780039364.01	0.825270	5.136266e-54	4.659434e-51
MsG0580027394.01	MsG0780039364.01	0.801740	7.808452e-49	3.757410e-46
MsG0680030313.01	MsG0780039364.01	0.805384	1.369881e-49	7.237231e-47
MsG0680030515.01	MsG0780039364.01	0.808232	3.425882e-50	1.948309e-47
MsG0280007075.01	MsG0780039364.01	0.819200	1.315604e-52	1.005702e-49
MsG0280007750.01	MsG0780039364.01	0.813036	3.135601e-51	2.025455e-48
MsG0780036517.01	MsG0780039364.01	0.809023	2.322434e-50	1.348476e-47
MsG0780038784.01	MsG0780039364.01	0.801025	1.094009e-48	5.170747e-46
MsG0780039155.01	MsG0780039364.01	0.816465	5.453544e-52	3.866962e-49
MsG0780039186.01	MsG0780039364.01	0.813167	2.935073e-51	1.902539e-48
MsG0780039362.01	MsG0780039364.01	0.868016	8.928323e-66	3.181011e-62
MsG0780039364.01	MsG0780039365.01	0.866790	2.203355e-65	7.519585e-62
MsG0780039364.01	MsG0780039975.01	0.833667	4.674660e-56	5.420332e-53
MsG0780039364.01	MsG0880042894.01	0.810976	8.818273e-51	5.393051e-48
MsG0780039364.01	MsG0880043814.01	0.804909	1.722510e-49	8.990863e-47
MsG0780039364.01	MsG0880043965.01	0.802269	6.078923e-49	2.964948e-46
MsG0780039364.01	MsG0880045668.01	0.807931	3.972419e-50	2.241069e-47
MsG0780039364.01	MsG0880047519.01	0.809915	1.494365e-50	8.885306e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039364.01.T01	MTR_7g071760	98.649	148	2	0	1	148	4	151	3.21e-110	309
MsG0780039364.01.T01	MTR_7g071790	97.973	148	3	0	1	148	4	151	9.10e-110	308
MsG0780039364.01.T01	MTR_7g071840	97.973	148	3	0	1	148	4	151	1.34e-109	308
MsG0780039364.01.T01	MTR_7g071970	97.297	148	4	0	1	148	4	151	1.59e-109	308
MsG0780039364.01.T01	MTR_7g071820	95.946	148	4	1	1	148	4	149	1.13e-104	295
MsG0780039364.01.T01	MTR_3g031270	50.336	149	63	5	2	148	23	162	6.13e-42	137
MsG0780039364.01.T01	MTR_3g072030	40.132	152	84	3	3	148	50	200	2.88e-30	108
MsG0780039364.01.T01	MTR_5g074500	37.908	153	83	7	3	148	6	153	2.13e-22	87.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780039364.01.T01	AT5G01740	53.947	152	60	5	3	148	15	162	6.78e-51	160
MsG0780039364.01.T01	AT3G10985	38.182	110	59	3	48	148	1	110	2.12e-16	70.5

Find 32 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATGATCTTCCCACTTTAA+TGG	0.211798	7:-62801242	None:intergenic
CCAATTGAAGTGACACTTCT+TGG	0.219369	7:-62801092	None:intergenic
GTCACTTCCTGGACTTGTTT+TGG	0.261773	7:+62801316	MsG0780039364.01.T01:CDS
GGTGGGAAGGACAAGCTTAT+TGG	0.263235	7:+62801135	MsG0780039364.01.T01:CDS
AAGATCACTTGCTAGCAATT+TGG	0.274100	7:-62800979	None:intergenic
AAGCTTATTGGGTTCATGTT+TGG	0.330474	7:+62801147	MsG0780039364.01.T01:CDS
GTGGGAAGGACAAGCTTATT+GGG	0.353672	7:+62801136	MsG0780039364.01.T01:CDS
CACATGATGAAGGTGTTAAC+AGG	0.390291	7:+62801035	MsG0780039364.01.T01:CDS
CCAAGAAGTGTCACTTCAAT+TGG	0.410079	7:+62801092	MsG0780039364.01.T01:CDS
CTTTATCGTAGGTCACTTCC+TGG	0.413885	7:+62801305	MsG0780039364.01.T01:CDS
AGGTCAAGGAACAATGGAAA+TGG	0.462620	7:+62800953	MsG0780039364.01.T01:CDS
GATCTTGAGTATTGGTTCCA+TGG	0.486528	7:+62800996	MsG0780039364.01.T01:CDS
TTTGTAGAGAATTTCAACTA+TGG	0.506167	7:-62800922	None:intergenic
TTAGAGAGTATTTCAATACA+TGG	0.507160	7:+62801198	MsG0780039364.01.T01:CDS
TAGCAAGTGATCTTGAGTAT+TGG	0.519406	7:+62800988	MsG0780039364.01.T01:CDS
GAGATTTGAGGCCATTAAAG+TGG	0.528323	7:+62801231	MsG0780039364.01.T01:CDS
TAAATAGCCAAAACAAGTCC+AGG	0.532508	7:-62801323	None:intergenic
TTCATCATGTGTTGACATTG+TGG	0.537051	7:-62801023	None:intergenic
AGATTTGAGGCCATTAAAGT+GGG	0.590336	7:+62801232	MsG0780039364.01.T01:CDS
ATCATGTGTTGACATTGTGG+TGG	0.597098	7:-62801020	None:intergenic
TTGTGTGATAACTGAAGGGT+GGG	0.598453	7:+62801118	MsG0780039364.01.T01:CDS
GTATTTCAATACATGGCTTG+TGG	0.599070	7:+62801205	MsG0780039364.01.T01:CDS
ACAATGTCAACACATGATGA+AGG	0.602094	7:+62801025	MsG0780039364.01.T01:CDS
GGCTTGTGGTGAGAGATTTG+AGG	0.614135	7:+62801219	MsG0780039364.01.T01:CDS
GTTGACATTGTGGTGGACCA+TGG	0.629400	7:-62801013	None:intergenic
ATTGTGTGATAACTGAAGGG+TGG	0.634073	7:+62801117	MsG0780039364.01.T01:CDS
GGTGATTGTGTGATAACTGA+AGG	0.637600	7:+62801113	MsG0780039364.01.T01:CDS
AGCAAGATAACATGACATTG+TGG	0.661544	7:+62801264	MsG0780039364.01.T01:CDS
AACCTCGTGATCTTTATCGT+AGG	0.678620	7:+62801294	MsG0780039364.01.T01:CDS
GTGATAACTGAAGGGTGGGA+AGG	0.682403	7:+62801122	MsG0780039364.01.T01:CDS
GTGATTGTGTGATAACTGAA+GGG	0.691615	7:+62801114	MsG0780039364.01.T01:CDS
GACCTACGATAAAGATCACG+AGG	0.717672	7:-62801296	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGTTTGGACAATGAAAAAT+GGG	+	Chr7:62801162-62801181	MsG0780039364.01.T01:CDS	25.0%
!	GAGAAACAAATCACAAAAAA+GGG	+	Chr7:62801057-62801076	MsG0780039364.01.T01:CDS	25.0%
!	TTTGTAGAGAATTTCAACTA+TGG	-	Chr7:62800925-62800944	None:intergenic	25.0%
!!!	TTAGAGAGTATTTCAATACA+TGG	+	Chr7:62801198-62801217	MsG0780039364.01.T01:CDS	25.0%
	CATGTTTGGACAATGAAAAA+TGG	+	Chr7:62801161-62801180	MsG0780039364.01.T01:CDS	30.0%
	GGAGAAACAAATCACAAAAA+AGG	+	Chr7:62801056-62801075	MsG0780039364.01.T01:CDS	30.0%
	TTATGATCTTCCCACTTTAA+TGG	-	Chr7:62801245-62801264	None:intergenic	30.0%
!!	ATTCTCTACAAAGCACTTTT+AGG	+	Chr7:62800933-62800952	MsG0780039364.01.T01:CDS	30.0%
	AAGCTTATTGGGTTCATGTT+TGG	+	Chr7:62801147-62801166	MsG0780039364.01.T01:CDS	35.0%
	ACAATGTCAACACATGATGA+AGG	+	Chr7:62801025-62801044	MsG0780039364.01.T01:CDS	35.0%
	AGCAAGATAACATGACATTG+TGG	+	Chr7:62801264-62801283	MsG0780039364.01.T01:CDS	35.0%
	GTATTTCAATACATGGCTTG+TGG	+	Chr7:62801205-62801224	MsG0780039364.01.T01:CDS	35.0%
	GTGATTGTGTGATAACTGAA+GGG	+	Chr7:62801114-62801133	MsG0780039364.01.T01:CDS	35.0%
	TAAATAGCCAAAACAAGTCC+AGG	-	Chr7:62801326-62801345	None:intergenic	35.0%
	TAGCAAGTGATCTTGAGTAT+TGG	+	Chr7:62800988-62801007	MsG0780039364.01.T01:CDS	35.0%
!	AAGATCACTTGCTAGCAATT+TGG	-	Chr7:62800982-62801001	None:intergenic	35.0%
!	AGATTTGAGGCCATTAAAGT+GGG	+	Chr7:62801232-62801251	MsG0780039364.01.T01:CDS	35.0%
!	TTCATCATGTGTTGACATTG+TGG	-	Chr7:62801026-62801045	None:intergenic	35.0%
!!	ACTTTTAGGTCAAGGAACAA+TGG	+	Chr7:62800947-62800966	MsG0780039364.01.T01:CDS	35.0%
!!	TACAAAGCACTTTTAGGTCA+AGG	+	Chr7:62800939-62800958	MsG0780039364.01.T01:CDS	35.0%
	AACCTCGTGATCTTTATCGT+AGG	+	Chr7:62801294-62801313	MsG0780039364.01.T01:CDS	40.0%
	AGGTCAAGGAACAATGGAAA+TGG	+	Chr7:62800953-62800972	MsG0780039364.01.T01:CDS	40.0%
	ATTGTGTGATAACTGAAGGG+TGG	+	Chr7:62801117-62801136	MsG0780039364.01.T01:CDS	40.0%
	CCAATTGAAGTGACACTTCT+TGG	-	Chr7:62801095-62801114	None:intergenic	40.0%
	GGTGATTGTGTGATAACTGA+AGG	+	Chr7:62801113-62801132	MsG0780039364.01.T01:CDS	40.0%
	TTGTGTGATAACTGAAGGGT+GGG	+	Chr7:62801118-62801137	MsG0780039364.01.T01:CDS	40.0%
!	ATCATGTGTTGACATTGTGG+TGG	-	Chr7:62801023-62801042	None:intergenic	40.0%
!	GAGATTTGAGGCCATTAAAG+TGG	+	Chr7:62801231-62801250	MsG0780039364.01.T01:CDS	40.0%
!!	CACATGATGAAGGTGTTAAC+AGG	+	Chr7:62801035-62801054	MsG0780039364.01.T01:CDS	40.0%
!!	CCAAGAAGTGTCACTTCAAT+TGG	+	Chr7:62801092-62801111	MsG0780039364.01.T01:CDS	40.0%
!!	GATCTTGAGTATTGGTTCCA+TGG	+	Chr7:62800996-62801015	MsG0780039364.01.T01:CDS	40.0%
	CTTTATCGTAGGTCACTTCC+TGG	+	Chr7:62801305-62801324	MsG0780039364.01.T01:CDS	45.0%
	GACCTACGATAAAGATCACG+AGG	-	Chr7:62801299-62801318	None:intergenic	45.0%
	GTGGGAAGGACAAGCTTATT+GGG	+	Chr7:62801136-62801155	MsG0780039364.01.T01:CDS	45.0%
!!	GTCACTTCCTGGACTTGTTT+TGG	+	Chr7:62801316-62801335	MsG0780039364.01.T01:CDS	45.0%
	GGCTTGTGGTGAGAGATTTG+AGG	+	Chr7:62801219-62801238	MsG0780039364.01.T01:CDS	50.0%
	GGTGGGAAGGACAAGCTTAT+TGG	+	Chr7:62801135-62801154	MsG0780039364.01.T01:CDS	50.0%
	GTGATAACTGAAGGGTGGGA+AGG	+	Chr7:62801122-62801141	MsG0780039364.01.T01:CDS	50.0%
!!	GTTGACATTGTGGTGGACCA+TGG	-	Chr7:62801016-62801035	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	62800900	62801346	62800900	ID=MsG0780039364.01;Name=MsG0780039364.01
Chr7	mRNA	62800900	62801346	62800900	ID=MsG0780039364.01.T01;Parent=MsG0780039364.01;Name=MsG0780039364.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr7	exon	62800900	62801346	62800900	ID=MsG0780039364.01.T01:exon:16939;Parent=MsG0780039364.01.T01
Chr7	CDS	62800900	62801346	62800900	ID=MsG0780039364.01.T01:cds;Parent=MsG0780039364.01.T01

Gene Sequence

>MsG0780039364.01.T01

ATGGTTGAAATGCAAAATAAGTCCATAGTTGAAATTCTCTACAAAGCACTTTTAGGTCAAGGAACAATGGAAATGGTAGCCAAATTGCTAGCAAGTGATCTTGAGTATTGGTTCCATGGTCCACCACAATGTCAACACATGATGAAGGTGTTAACAGGAGAAACAAATCACAAAAAAGGGTTCAAATTTGAGCCAAGAAGTGTCACTTCAATTGGTGATTGTGTGATAACTGAAGGGTGGGAAGGACAAGCTTATTGGGTTCATGTTTGGACAATGAAAAATGGGTTAATTACACAATTTAGAGAGTATTTCAATACATGGCTTGTGGTGAGAGATTTGAGGCCATTAAAGTGGGAAGATCATAAGCAAGATAACATGACATTGTGGAGAAGTCAACCTCGTGATCTTTATCGTAGGTCACTTCCTGGACTTGTTTTGGCTATTTAG

Protein sequence

>MsG0780039364.01.T01

MVEMQNKSIVEILYKALLGQGTMEMVAKLLASDLEYWFHGPPQCQHMMKVLTGETNHKKGFKFEPRSVTSIGDCVITEGWEGQAYWVHVWTMKNGLITQFREYFNTWLVVRDLRPLKWEDHKQDNMTLWRSQPRDLYRRSLPGLVLAI*